MCCC1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCCC1
Entrez Gene ID	513504
Full Name	methylcrotonoyl-CoA carboxylase 1
General protein information	Preferred Names methylcrotonoyl-CoA carboxylase 1 Names methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial methylcrotonoyl-CoA carboxylase 1 (alpha) methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010801298.1	XP_010799600.1	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X3
NM_001205758.1	NP_001192687.1	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
XM_010801297.2	XP_010799599.1	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1
XM_015472184.1	XP_015327670.1	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X2
XM_015472184.2	XP_015327670.1	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X2
XM_010801297.3	XP_010799599.1	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mccc1	NP_001009653.1
Gallus gallus (chicken)	MCCC1	NP_001006551.1
Drosophila melanogaster (fruit fly)	CG2118	NP_001247391.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010228	XP_319416.4
Neurospora crassa	NCU00591	XP_965731.1
Oryza sativa (rice)	Os12g0605800	NP_001067226.1
Mus musculus (house mouse)	Mccc1	NP_076133.3
Danio rerio (zebrafish)	mccc1	NP_001038380.1
Magnaporthe oryzae (rice blast fungus)	MGG_10320	XP_003720502.1
Xenopus tropicalis (tropical clawed frog)	mccc1	NP_001006691.1
Homo sapiens (human)	MCCC1	NP_064551.3
	LOC708258	XP_001096717.1
Pan troglodytes (chimpanzee)	MCCC1	XP_516898.3
Canis lupus familiaris (dog)	MCCC1	XP_535817.2
Bos taurus (cattle)	MCCC1	NP_001192687.1
Caenorhabditis elegans (roundworm)	F32B6.2	NP_501777.2
Arabidopsis thaliana (thale cress)	MCCA	NP_849583.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MCCC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCCC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa48644	XM_010801297.3 Latest version!	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa48645	XM_010801298.1 Latest version!	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa39674	NM_001205758.1 Latest version!	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa87759	XM_015472184.2 Latest version!	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OBa87759	XM_015472184.1	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa48644	XM_010801297.2	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCCC1 ( XM_010801297.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799599.1 Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa48644
Clone ID Related Accession (Same CDS sequence)	XM_010801297.2 , XM_010801297.3
Accession Version	XM_010801297.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAAC CACAGGCAGA TGAAGCCTAT CTCATCGGCC CCGCTCCCTC TCAGCAGAGC 
TACCTTTCTA TGGAAAAAAT CATTCAAGTG GCCAAGATCT CTGCCGCACA GGCTATCCAT 
CCAGGATATG GCTTTCTCTC AGAAAACATG GAATTTGCCG AACTGTGTAA GCAAGAAGGA 
ATTATTTTTG TAGGTCCTCC TTCATCAGCA ATTAGAGACA TGGGTATAAA GAGCACATCC 
AAATCCATCA TGGCTGCTGC TGGAGTGCCT GTGGTGGAGG GTTATCACGG GGAGGACCAG 
TCAGACCAAT GCCTGAAAGA GCACGCTAGG ACAATCGGTT ACCCCGTCAT GATTAAAGCC 
GTCCGTGGCG GAGGAGGAAA AGGTATGAGG ATCGTTAGAT CTGAAAAAGA ATTTCAAGAG 
CAGTTAGAAT CAGCACGGAG AGAAGCTAAG AAGTCTTTCA ATGATGATGC CATGCTGATT 
GAGAAGTTTG TGGACACACC AAGGCATGTA GAAGTGCAGG TGTTTGGTGA CCACCATGGC 
AATGCCGTGT ACTTGTTTGA AAGAGACTGT AGTGTACAGA GAAGACATCA GAAGATCATT 
GAAGAGGCCC CAGGGCCTGG TATTAAACCT GAAGTAAGAA AAAAGCTTGG AGAAGCTGCA 
GTCAGAGCTG CTAAAGCTGT AAATTATGTT GGTGCAGGAA CCGTGGAGTT TATCATGGAC 
TCAAAGCACA ATTTCTACTT TATGGAGATG AATACAAGGC TGCAAGTGGA ACATCCTGTC 
ACCGAGATGA TCACAGGAAC TGACTTGGTG GAATGGCAAC TTAGGATTGC AGCAGGAGAG 
AAGATTCCTT TGAGCCAGGA AGAAATAACT CTGCGGGGCC ATGCCTTTGA AGCTAGAATA 
TATGCAGAAG ATCCTAACAA TAACTTCATG CCGGGGGCCG GACCACTAGT GCATCTCTCC 
ACCCCTCGAG CAGACCTTAC CACTAGGATT GAAACTGGAG TACGGCAAGG AGATGAAGTT 
TCAATGCATT ATGACCCCAT GATTGCAAAG CTGGTTGTGT GGGCTGCTGA TCGCCAGGCA 
GCCTTGACGA AACTTAAGTA CAGCCTTCGA CAGTACAATA TTGTTGGACT GCACACCAAC 
ATTGACTTCC TCCTTAGCCT GTCTGGCCAC CCGGAATTTG AAGCTGGGAA CGTGCACACT 
GATTTCATCG CTCAACACCG CAAAGAACTG TTGCCCGACC GGAAGACCAC AGCCAAGGAG 
TTTTTATGTC ACGCAGCTCT GGCCCTCATC CTCAAGGAGA AAGCTGTGTC TGATGTCTTC 
AAAATTCAGA CACAAGATCA ATACTCTCCT TTTGCATCCA GCAGTGGAAG AAGACTGAAT 
ATCTGTTACT CTAGAAACAT GACTCTTAGG GATGGTAAAA ACAATGTAGC CATAGCTGTA 
ACATATAACC GTGATGGATC ATATAGCATG CAGATTGAAG ATAAAACTTT CCATGTCCTT 
GGTGATCTTT GCAGTGAGGG AGACTGCACT TATCTGAAAT CTTCTGTTAA TGGAGTTGCT 
AGTAAAATTA AGCTAATTAT CCTGAAAAAC ACTATTTACC TATTTTCCAT GGATGGAAGT 
ATTCAGATTG GCATTCCGGT TCCCCGATAC TTGTCTTCAG CAAGCTCAGG AGAAACCCAA 
GGGGGTGCTG TAGCTCCTAT GACTGGAACT GTTGAAAAGG TGTTTGTGAA AGCTGGAGAC 
AGAGTAAAAG CTGGAGACTC TCTGATGGTT ATGATTGCCA TGAAAATGGA ACATACCATA 
AAGGCGCCAA AGGATGGCAC AATCAAGAAG GTATTCTACA AAGAAGGTTC TCAGGCCAAC 
AGACACGCTC CTTTAGTCGA GTTTGAGGAG GAAGACTCTG ACGAAAGGGA AGCAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799599.1
CDS	242..2161
Translation	MQQPQADEAYLIGPAPSQQSYLSMEKIIQVAKISAAQAIHPGYGFLSENMEFAELCKQEGIIFVGPPSSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARTIGYPVMIKAVRGGGGKGMRIVRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPGPGIKPEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLRGHAFEARIYAEDPNNNFMPGAGPLVHLSTPRADLTTRIETGVRQGDEVSMHYDPMIAKLVVWAADRQAALTKLKYSLRQYNIVGLHTNIDFLLSLSGHPEFEAGNVHTDFIAQHRKELLPDRKTTAKEFLCHAALALILKEKAVSDVFKIQTQDQYSPFASSSGRRLNICYSRNMTLRDGKNNVAIAVTYNRDGSYSMQIEDKTFHVLGDLCSEGDCTYLKSSVNGVASKIKLIILKNTIYLFSMDGSIQIGIPVPRYLSSASSGETQGGAVAPMTGTVEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGTIKKVFYKEGSQANRHAPLVEFEEEDSDEREAD

Target ORF information:

RefSeq Version	XM_010801297.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801297.2

ORF Insert Sequence:

ATGCAACAAC CACAGGCAGA TGAAGCCTAT CTCATCGGCC CCGCTCCCTC TCAGCAGAGC 
TACCTTTCTA TGGAAAAAAT CATTCAAGTG GCCAAGATCT CTGCCGCACA GGCTATCCAT 
CCAGGATATG GCTTTCTCTC AGAAAACATG GAATTTGCCG AACTGTGTAA GCAAGAAGGA 
ATTATTTTTG TAGGTCCTCC TTCATCAGCA ATTAGAGACA TGGGTATAAA GAGCACATCC 
AAATCCATCA TGGCTGCTGC TGGAGTGCCT GTGGTGGAGG GTTATCACGG GGAGGACCAG 
TCAGACCAAT GCCTGAAAGA GCACGCTAGG ACAATCGGTT ACCCCGTCAT GATTAAAGCC 
GTCCGTGGCG GAGGAGGAAA AGGTATGAGG ATCGTTAGAT CTGAAAAAGA ATTTCAAGAG 
CAGTTAGAAT CAGCACGGAG AGAAGCTAAG AAGTCTTTCA ATGATGATGC CATGCTGATT 
GAGAAGTTTG TGGACACACC AAGGCATGTA GAAGTGCAGG TGTTTGGTGA CCACCATGGC 
AATGCCGTGT ACTTGTTTGA AAGAGACTGT AGTGTACAGA GAAGACATCA GAAGATCATT 
GAAGAGGCCC CAGGGCCTGG TATTAAACCT GAAGTAAGAA AAAAGCTTGG AGAAGCTGCA 
GTCAGAGCTG CTAAAGCTGT AAATTATGTT GGTGCAGGAA CCGTGGAGTT TATCATGGAC 
TCAAAGCACA ATTTCTACTT TATGGAGATG AATACAAGGC TGCAAGTGGA ACATCCTGTC 
ACCGAGATGA TCACAGGAAC TGACTTGGTG GAATGGCAAC TTAGGATTGC AGCAGGAGAG 
AAGATTCCTT TGAGCCAGGA AGAAATAACT CTGCGGGGCC ATGCCTTTGA AGCTAGAATA 
TATGCAGAAG ATCCTAACAA TAACTTCATG CCGGGGGCCG GACCACTAGT GCATCTCTCC 
ACCCCTCGAG CAGACCTTAC CACTAGGATT GAAACTGGAG TACGGCAAGG AGATGAAGTT 
TCAATGCATT ATGACCCCAT GATTGCAAAG CTGGTTGTGT GGGCTGCTGA TCGCCAGGCA 
GCCTTGACGA AACTTAAGTA CAGCCTTCGA CAGTACAATA TTGTTGGACT GCACACCAAC 
ATTGACTTCC TCCTTAGCCT GTCTGGCCAC CCGGAATTTG AAGCTGGGAA CGTGCACACT 
GATTTCATCG CTCAACACCG CAAAGAACTG TTGCCCGACC GGAAGACCAC AGCCAAGGAG 
TTTTTATGTC ACGCAGCTCT GGCCCTCATC CTCAAGGAGA AAGCTGTGTC TGATGTCTTC 
AAAATTCAGA CACAAGATCA ATACTCTCCT TTTGCATCCA GCAGTGGAAG AAGACTGAAT 
ATCTGTTACT CTAGAAACAT GACTCTTAGG GATGGTAAAA ACAATGTAGC CATAGCTGTA 
ACATATAACC GTGATGGATC ATATAGCATG CAGATTGAAG ATAAAACTTT CCATGTCCTT 
GGTGATCTTT GCAGTGAGGG AGACTGCACT TATCTGAAAT CTTCTGTTAA TGGAGTTGCT 
AGTAAAATTA AGCTAATTAT CCTGAAAAAC ACTATTTACC TATTTTCCAT GGATGGAAGT 
ATTCAGATTG GCATTCCGGT TCCCCGATAC TTGTCTTCAG CAAGCTCAGG AGAAACCCAA 
GGGGGTGCTG TAGCTCCTAT GACTGGAACT GTTGAAAAGG TGTTTGTGAA AGCTGGAGAC 
AGAGTAAAAG CTGGAGACTC TCTGATGGTT ATGATTGCCA TGAAAATGGA ACATACCATA 
AAGGCGCCAA AGGATGGCAC AATCAAGAAG GTATTCTACA AAGAAGGTTC TCAGGCCAAC 
AGACACGCTC CTTTAGTCGA GTTTGAGGAG GAAGACTCTG ACGAAAGGGA AGCAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC1 ( XM_010801297.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799599.1 Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa48644
Clone ID Related Accession (Same CDS sequence)	XM_010801297.2 , XM_010801297.3
Accession Version	XM_010801297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAAC CACAGGCAGA TGAAGCCTAT CTCATCGGCC CCGCTCCCTC TCAGCAGAGC 
TACCTTTCTA TGGAAAAAAT CATTCAAGTG GCCAAGATCT CTGCCGCACA GGCTATCCAT 
CCAGGATATG GCTTTCTCTC AGAAAACATG GAATTTGCCG AACTGTGTAA GCAAGAAGGA 
ATTATTTTTG TAGGTCCTCC TTCATCAGCA ATTAGAGACA TGGGTATAAA GAGCACATCC 
AAATCCATCA TGGCTGCTGC TGGAGTGCCT GTGGTGGAGG GTTATCACGG GGAGGACCAG 
TCAGACCAAT GCCTGAAAGA GCACGCTAGG ACAATCGGTT ACCCCGTCAT GATTAAAGCC 
GTCCGTGGCG GAGGAGGAAA AGGTATGAGG ATCGTTAGAT CTGAAAAAGA ATTTCAAGAG 
CAGTTAGAAT CAGCACGGAG AGAAGCTAAG AAGTCTTTCA ATGATGATGC CATGCTGATT 
GAGAAGTTTG TGGACACACC AAGGCATGTA GAAGTGCAGG TGTTTGGTGA CCACCATGGC 
AATGCCGTGT ACTTGTTTGA AAGAGACTGT AGTGTACAGA GAAGACATCA GAAGATCATT 
GAAGAGGCCC CAGGGCCTGG TATTAAACCT GAAGTAAGAA AAAAGCTTGG AGAAGCTGCA 
GTCAGAGCTG CTAAAGCTGT AAATTATGTT GGTGCAGGAA CCGTGGAGTT TATCATGGAC 
TCAAAGCACA ATTTCTACTT TATGGAGATG AATACAAGGC TGCAAGTGGA ACATCCTGTC 
ACCGAGATGA TCACAGGAAC TGACTTGGTG GAATGGCAAC TTAGGATTGC AGCAGGAGAG 
AAGATTCCTT TGAGCCAGGA AGAAATAACT CTGCGGGGCC ATGCCTTTGA AGCTAGAATA 
TATGCAGAAG ATCCTAACAA TAACTTCATG CCGGGGGCCG GACCACTAGT GCATCTCTCC 
ACCCCTCGAG CAGACCTTAC CACTAGGATT GAAACTGGAG TACGGCAAGG AGATGAAGTT 
TCAATGCATT ATGACCCCAT GATTGCAAAG CTGGTTGTGT GGGCTGCTGA TCGCCAGGCA 
GCCTTGACGA AACTTAAGTA CAGCCTTCGA CAGTACAATA TTGTTGGACT GCACACCAAC 
ATTGACTTCC TCCTTAGCCT GTCTGGCCAC CCGGAATTTG AAGCTGGGAA CGTGCACACT 
GATTTCATCG CTCAACACCG CAAAGAACTG TTGCCCGACC GGAAGACCAC AGCCAAGGAG 
TTTTTATGTC ACGCAGCTCT GGCCCTCATC CTCAAGGAGA AAGCTGTGTC TGATGTCTTC 
AAAATTCAGA CACAAGATCA ATACTCTCCT TTTGCATCCA GCAGTGGAAG AAGACTGAAT 
ATCTGTTACT CTAGAAACAT GACTCTTAGG GATGGTAAAA ACAATGTAGC CATAGCTGTA 
ACATATAACC GTGATGGATC ATATAGCATG CAGATTGAAG ATAAAACTTT CCATGTCCTT 
GGTGATCTTT GCAGTGAGGG AGACTGCACT TATCTGAAAT CTTCTGTTAA TGGAGTTGCT 
AGTAAAATTA AGCTAATTAT CCTGAAAAAC ACTATTTACC TATTTTCCAT GGATGGAAGT 
ATTCAGATTG GCATTCCGGT TCCCCGATAC TTGTCTTCAG CAAGCTCAGG AGAAACCCAA 
GGGGGTGCTG TAGCTCCTAT GACTGGAACT GTTGAAAAGG TGTTTGTGAA AGCTGGAGAC 
AGAGTAAAAG CTGGAGACTC TCTGATGGTT ATGATTGCCA TGAAAATGGA ACATACCATA 
AAGGCGCCAA AGGATGGCAC AATCAAGAAG GTATTCTACA AAGAAGGTTC TCAGGCCAAC 
AGACACGCTC CTTTAGTCGA GTTTGAGGAG GAAGACTCTG ACGAAAGGGA AGCAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799599.1
CDS	245..2164
Translation	MQQPQADEAYLIGPAPSQQSYLSMEKIIQVAKISAAQAIHPGYGFLSENMEFAELCKQEGIIFVGPPSSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARTIGYPVMIKAVRGGGGKGMRIVRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPGPGIKPEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLRGHAFEARIYAEDPNNNFMPGAGPLVHLSTPRADLTTRIETGVRQGDEVSMHYDPMIAKLVVWAADRQAALTKLKYSLRQYNIVGLHTNIDFLLSLSGHPEFEAGNVHTDFIAQHRKELLPDRKTTAKEFLCHAALALILKEKAVSDVFKIQTQDQYSPFASSSGRRLNICYSRNMTLRDGKNNVAIAVTYNRDGSYSMQIEDKTFHVLGDLCSEGDCTYLKSSVNGVASKIKLIILKNTIYLFSMDGSIQIGIPVPRYLSSASSGETQGGAVAPMTGTVEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGTIKKVFYKEGSQANRHAPLVEFEEEDSDEREAD

Target ORF information:

RefSeq Version	XM_010801297.3
Organism	Bos taurus(cattle)
Definition	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801297.3

ORF Insert Sequence:

ATGCAACAAC CACAGGCAGA TGAAGCCTAT CTCATCGGCC CCGCTCCCTC TCAGCAGAGC 
TACCTTTCTA TGGAAAAAAT CATTCAAGTG GCCAAGATCT CTGCCGCACA GGCTATCCAT 
CCAGGATATG GCTTTCTCTC AGAAAACATG GAATTTGCCG AACTGTGTAA GCAAGAAGGA 
ATTATTTTTG TAGGTCCTCC TTCATCAGCA ATTAGAGACA TGGGTATAAA GAGCACATCC 
AAATCCATCA TGGCTGCTGC TGGAGTGCCT GTGGTGGAGG GTTATCACGG GGAGGACCAG 
TCAGACCAAT GCCTGAAAGA GCACGCTAGG ACAATCGGTT ACCCCGTCAT GATTAAAGCC 
GTCCGTGGCG GAGGAGGAAA AGGTATGAGG ATCGTTAGAT CTGAAAAAGA ATTTCAAGAG 
CAGTTAGAAT CAGCACGGAG AGAAGCTAAG AAGTCTTTCA ATGATGATGC CATGCTGATT 
GAGAAGTTTG TGGACACACC AAGGCATGTA GAAGTGCAGG TGTTTGGTGA CCACCATGGC 
AATGCCGTGT ACTTGTTTGA AAGAGACTGT AGTGTACAGA GAAGACATCA GAAGATCATT 
GAAGAGGCCC CAGGGCCTGG TATTAAACCT GAAGTAAGAA AAAAGCTTGG AGAAGCTGCA 
GTCAGAGCTG CTAAAGCTGT AAATTATGTT GGTGCAGGAA CCGTGGAGTT TATCATGGAC 
TCAAAGCACA ATTTCTACTT TATGGAGATG AATACAAGGC TGCAAGTGGA ACATCCTGTC 
ACCGAGATGA TCACAGGAAC TGACTTGGTG GAATGGCAAC TTAGGATTGC AGCAGGAGAG 
AAGATTCCTT TGAGCCAGGA AGAAATAACT CTGCGGGGCC ATGCCTTTGA AGCTAGAATA 
TATGCAGAAG ATCCTAACAA TAACTTCATG CCGGGGGCCG GACCACTAGT GCATCTCTCC 
ACCCCTCGAG CAGACCTTAC CACTAGGATT GAAACTGGAG TACGGCAAGG AGATGAAGTT 
TCAATGCATT ATGACCCCAT GATTGCAAAG CTGGTTGTGT GGGCTGCTGA TCGCCAGGCA 
GCCTTGACGA AACTTAAGTA CAGCCTTCGA CAGTACAATA TTGTTGGACT GCACACCAAC 
ATTGACTTCC TCCTTAGCCT GTCTGGCCAC CCGGAATTTG AAGCTGGGAA CGTGCACACT 
GATTTCATCG CTCAACACCG CAAAGAACTG TTGCCCGACC GGAAGACCAC AGCCAAGGAG 
TTTTTATGTC ACGCAGCTCT GGCCCTCATC CTCAAGGAGA AAGCTGTGTC TGATGTCTTC 
AAAATTCAGA CACAAGATCA ATACTCTCCT TTTGCATCCA GCAGTGGAAG AAGACTGAAT 
ATCTGTTACT CTAGAAACAT GACTCTTAGG GATGGTAAAA ACAATGTAGC CATAGCTGTA 
ACATATAACC GTGATGGATC ATATAGCATG CAGATTGAAG ATAAAACTTT CCATGTCCTT 
GGTGATCTTT GCAGTGAGGG AGACTGCACT TATCTGAAAT CTTCTGTTAA TGGAGTTGCT 
AGTAAAATTA AGCTAATTAT CCTGAAAAAC ACTATTTACC TATTTTCCAT GGATGGAAGT 
ATTCAGATTG GCATTCCGGT TCCCCGATAC TTGTCTTCAG CAAGCTCAGG AGAAACCCAA 
GGGGGTGCTG TAGCTCCTAT GACTGGAACT GTTGAAAAGG TGTTTGTGAA AGCTGGAGAC 
AGAGTAAAAG CTGGAGACTC TCTGATGGTT ATGATTGCCA TGAAAATGGA ACATACCATA 
AAGGCGCCAA AGGATGGCAC AATCAAGAAG GTATTCTACA AAGAAGGTTC TCAGGCCAAC 
AGACACGCTC CTTTAGTCGA GTTTGAGGAG GAAGACTCTG ACGAAAGGGA AGCAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC1 ( XM_010801298.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799600.1 Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa48645
Clone ID Related Accession (Same CDS sequence)	XM_010801298.1
Accession Version	XM_010801298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTGG AAGCAAAGAA CAAGGAAGTA TGCAACAACC ACAGGCAGAT GAAGCCTATC 
TCATCGGCCC CGCTCCCTCT CAGCAGAGCT ACCTTTCTAT GGAAAAAATC ATTCAAGTGG 
CCAAGATCTC TGCCGCACAG CACATCCAAA TCCATCATGG CTGCTGCTGG AGTGCCTGTG 
GTGGAGGGTT ATCACGGGGA GGACCAGTCA GACCAATGCC TGAAAGAGCA CGCTAGGACA 
ATCGGTTACC CCGTCATGAT TAAAGCCGTC CGTGGCGGAG GAGGAAAAGG TATGAGGATC 
GTTAGATCTG AAAAAGAATT TCAAGAGCAG TTAGAATCAG CACGGAGAGA AGCTAAGAAG 
TCTTTCAATG ATGATGCCAT GCTGATTGAG AAGTTTGTGG ACACACCAAG GCATGTAGAA 
GTGCAGGTGT TTGGTGACCA CCATGGCAAT GCCGTGTACT TGTTTGAAAG AGACTGTAGT 
GTACAGAGAA GACATCAGAA GATCATTGAA GAGGCCCCAG GGCCTGGTAT TAAACCTGAA 
GTAAGAAAAA AGCTTGGAGA AGCTGCAGTC AGAGCTGCTA AAGCTGTAAA TTATGTTGGT 
GCAGGAACCG TGGAGTTTAT CATGGACTCA AAGCACAATT TCTACTTTAT GGAGATGAAT 
ACAAGGCTGC AAGTGGAACA TCCTGTCACC GAGATGATCA CAGGAACTGA CTTGGTGGAA 
TGGCAACTTA GGATTGCAGC AGGAGAGAAG ATTCCTTTGA GCCAGGAAGA AATAACTCTG 
CGGGGCCATG CCTTTGAAGC TAGAATATAT GCAGAAGATC CTAACAATAA CTTCATGCCG 
GGGGCCGGAC CACTAGTGCA TCTCTCCACC CCTCGAGCAG ACCTTACCAC TAGGATTGAA 
ACTGGAGTAC GGCAAGGAGA TGAAGTTTCA ATGCATTATG ACCCCATGAT TGCAAAGCTG 
GTTGTGTGGG CTGCTGATCG CCAGGCAGCC TTGACGAAAC TTAAGTACAG CCTTCGACAG 
TACAATATTG TTGGACTGCA CACCAACATT GACTTCCTCC TTAGCCTGTC TGGCCACCCG 
GAATTTGAAG CTGGGAACGT GCACACTGAT TTCATCGCTC AACACCGCAA AGAACTGTTG 
CCCGACCGGA AGACCACAGC CAAGGAGTTT TTATGTCACG CAGCTCTGGC CCTCATCCTC 
AAGGAGAAAG CTGTGTCTGA TGTCTTCAAA ATTCAGACAC AAGATCAATA CTCTCCTTTT 
GCATCCAGCA GTGGAAGAAG ACTGAATATC TGTTACTCTA GAAACATGAC TCTTAGGGAT 
GGTAAAAACA ATGTAGCCAT AGCTGTAACA TATAACCGTG ATGGATCATA TAGCATGCAG 
ATTGAAGATA AAACTTTCCA TGTCCTTGGT GATCTTTGCA GTGAGGGAGA CTGCACTTAT 
CTGAAATCTT CTGTTAATGG AGTTGCTAGT AAAATTAAGC TAATTATCCT GAAAAACACT 
ATTTACCTAT TTTCCATGGA TGGAAGTATT CAGATTGGCA TTCCGGTTCC CCGATACTTG 
TCTTCAGCAA GCTCAGGAGA AACCCAAGGG GGTGCTGTAG CTCCTATGAC TGGAACTGTT 
GAAAAGGTGT TTGTGAAAGC TGGAGACAGA GTAAAAGCTG GAGACTCTCT GATGGTTATG 
ATTGCCATGA AAATGGAACA TACCATAAAG GCGCCAAAGG ATGGCACAAT CAAGAAGGTA 
TTCTACAAAG AAGGTTCTCA GGCCAACAGA CACGCTCCTT TAGTCGAGTT TGAGGAGGAA 
GACTCTGACG AAAGGGAAGC AGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799600.1
CDS	1..1827
Translation	MGLEAKNKEVCNNHRQMKPISSAPLPLSRATFLWKKSFKWPRSLPHSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARTIGYPVMIKAVRGGGGKGMRIVRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPGPGIKPEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLRGHAFEARIYAEDPNNNFMPGAGPLVHLSTPRADLTTRIETGVRQGDEVSMHYDPMIAKLVVWAADRQAALTKLKYSLRQYNIVGLHTNIDFLLSLSGHPEFEAGNVHTDFIAQHRKELLPDRKTTAKEFLCHAALALILKEKAVSDVFKIQTQDQYSPFASSSGRRLNICYSRNMTLRDGKNNVAIAVTYNRDGSYSMQIEDKTFHVLGDLCSEGDCTYLKSSVNGVASKIKLIILKNTIYLFSMDGSIQIGIPVPRYLSSASSGETQGGAVAPMTGTVEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGTIKKVFYKEGSQANRHAPLVEFEEEDSDEREAD

Target ORF information:

RefSeq Version	XM_010801298.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801298.1

ORF Insert Sequence:

ATGGGCTTGG AAGCAAAGAA CAAGGAAGTA TGCAACAACC ACAGGCAGAT GAAGCCTATC 
TCATCGGCCC CGCTCCCTCT CAGCAGAGCT ACCTTTCTAT GGAAAAAATC ATTCAAGTGG 
CCAAGATCTC TGCCGCACAG CACATCCAAA TCCATCATGG CTGCTGCTGG AGTGCCTGTG 
GTGGAGGGTT ATCACGGGGA GGACCAGTCA GACCAATGCC TGAAAGAGCA CGCTAGGACA 
ATCGGTTACC CCGTCATGAT TAAAGCCGTC CGTGGCGGAG GAGGAAAAGG TATGAGGATC 
GTTAGATCTG AAAAAGAATT TCAAGAGCAG TTAGAATCAG CACGGAGAGA AGCTAAGAAG 
TCTTTCAATG ATGATGCCAT GCTGATTGAG AAGTTTGTGG ACACACCAAG GCATGTAGAA 
GTGCAGGTGT TTGGTGACCA CCATGGCAAT GCCGTGTACT TGTTTGAAAG AGACTGTAGT 
GTACAGAGAA GACATCAGAA GATCATTGAA GAGGCCCCAG GGCCTGGTAT TAAACCTGAA 
GTAAGAAAAA AGCTTGGAGA AGCTGCAGTC AGAGCTGCTA AAGCTGTAAA TTATGTTGGT 
GCAGGAACCG TGGAGTTTAT CATGGACTCA AAGCACAATT TCTACTTTAT GGAGATGAAT 
ACAAGGCTGC AAGTGGAACA TCCTGTCACC GAGATGATCA CAGGAACTGA CTTGGTGGAA 
TGGCAACTTA GGATTGCAGC AGGAGAGAAG ATTCCTTTGA GCCAGGAAGA AATAACTCTG 
CGGGGCCATG CCTTTGAAGC TAGAATATAT GCAGAAGATC CTAACAATAA CTTCATGCCG 
GGGGCCGGAC CACTAGTGCA TCTCTCCACC CCTCGAGCAG ACCTTACCAC TAGGATTGAA 
ACTGGAGTAC GGCAAGGAGA TGAAGTTTCA ATGCATTATG ACCCCATGAT TGCAAAGCTG 
GTTGTGTGGG CTGCTGATCG CCAGGCAGCC TTGACGAAAC TTAAGTACAG CCTTCGACAG 
TACAATATTG TTGGACTGCA CACCAACATT GACTTCCTCC TTAGCCTGTC TGGCCACCCG 
GAATTTGAAG CTGGGAACGT GCACACTGAT TTCATCGCTC AACACCGCAA AGAACTGTTG 
CCCGACCGGA AGACCACAGC CAAGGAGTTT TTATGTCACG CAGCTCTGGC CCTCATCCTC 
AAGGAGAAAG CTGTGTCTGA TGTCTTCAAA ATTCAGACAC AAGATCAATA CTCTCCTTTT 
GCATCCAGCA GTGGAAGAAG ACTGAATATC TGTTACTCTA GAAACATGAC TCTTAGGGAT 
GGTAAAAACA ATGTAGCCAT AGCTGTAACA TATAACCGTG ATGGATCATA TAGCATGCAG 
ATTGAAGATA AAACTTTCCA TGTCCTTGGT GATCTTTGCA GTGAGGGAGA CTGCACTTAT 
CTGAAATCTT CTGTTAATGG AGTTGCTAGT AAAATTAAGC TAATTATCCT GAAAAACACT 
ATTTACCTAT TTTCCATGGA TGGAAGTATT CAGATTGGCA TTCCGGTTCC CCGATACTTG 
TCTTCAGCAA GCTCAGGAGA AACCCAAGGG GGTGCTGTAG CTCCTATGAC TGGAACTGTT 
GAAAAGGTGT TTGTGAAAGC TGGAGACAGA GTAAAAGCTG GAGACTCTCT GATGGTTATG 
ATTGCCATGA AAATGGAACA TACCATAAAG GCGCCAAAGG ATGGCACAAT CAAGAAGGTA 
TTCTACAAAG AAGGTTCTCA GGCCAACAGA CACGCTCCTT TAGTCGAGTT TGAGGAGGAA 
GACTCTGACG AAAGGGAAGC AGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC1 ( NM_001205758.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192687.1 Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), mRNA.

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CloneID	OBa39674
Clone ID Related Accession (Same CDS sequence)	NM_001205758.1
Accession Version	NM_001205758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-24
Organism	Bos taurus(cattle)
Product	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000001.1. On Apr 20, 2011 this sequence version replaced XM_591194.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571255.14605.1, SRR5571256.5122.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCGGCGG CCTCTTTGGT GTCGGTGCTG CTCGTGGCGG CGGAGAGGCA CCGGTGGCAG 
CGAGTCCCGC GCTTGCTGCT GCCGCCGAGG GGATGGGCTT GGAAGCAAAG AACAAGGAAG 
TATGCAACAA CCACAGGACA AAACATTTCC AAGGTCCTCA TTGCAAACAG AGGAGAAATT 
GCCTGCAGGG TGATACGGAC AGCCAAAAAA ATGGGTGTAC AGTCTGTGGC TGTTTATAGT 
GAGGCCGACA GGAATTCCAT GCATGTTGAC ATGGCAGATG AAGCCTATCT CATCGGCCCC 
GCTCCCTCTC AGCAGAGCTA CCTTTCTATG GAAAAAATCA TTCAAGTGGC CAAGATCTCT 
GCCGCACAGG CTATCCATCC AGGATATGGC TTTCTCTCAG AAAACATGGA ATTTGCCGAA 
CTGTGTAAGC AAGAAGGAAT TATTTTTGTA GGTCCTCCTT CATCAGCAAT TAGAGACATG 
GGTATAAAGA GCACATCCAA ATCCATCATG GCTGCTGCTG GAGTGCCTGT GGTGGAGGGT 
TATCACGGGG AGGACCAGTC AGACCAATGC CTGAAAGAGC ACGCTAGGAC AATCGGTTAC 
CCCGTCATGA TTAAAGCCGT CCGTGGCGGA GGAGGAAAAG GTATGAGGAT CGTTAGATCT 
GAAAAAGAAT TTCAAGAGCA GTTAGAATCA GCACGGAGAG AAGCTAAGAA GTCTTTCAAT 
GATGATGCCA TGCTGATTGA GAAGTTTGTG GACACACCAA GGCATGTAGA AGTGCAGGTG 
TTTGGTGACC ACCATGGCAA TGCCGTGTAC TTGTTTGAAA GAGACTGTAG TGTACAGAGA 
AGACATCAGA AGATCATTGA AGAGGCCCCA GGGCCTGGTA TTAAACCTGA AGTAAGAAAA 
AAGCTTGGAG AAGCTGCAGT CAGAGCTGCT AAAGCTGTAA ATTATGTTGG TGCAGGAACC 
GTGGAGTTTA TCATGGACTC AAAGCACAAT TTCTACTTTA TGGAGATGAA TACAAGGCTG 
CAAGTGGAAC ATCCTGTCAC CGAGATGATC ACAGGAACTG ACTTGGTGGA ATGGCAACTT 
AGGATTGCAG CAGGAGAGAA GATTCCTTTG AGCCAGGAAG AAATAACTCT GCGGGGCCAT 
GCCTTTGAAG CTAGAATATA TGCAGAAGAT CCTAACAATA ACTTCATGCC GGGGGCCGGA 
CCACTAGTGC ATCTCTCCAC CCCTCGAGCA GACCTTACCA CTAGGATTGA AACTGGAGTA 
CGGCAAGGAG ATGAAGTTTC AATGCATTAT GACCCCATGA TTGCAAAGCT GGTTGTGTGG 
GCTGCTGATC GCCAGGCAGC CTTGACGAAA CTTAAGTACA GCCTTCGACA GTACAATATT 
GTTGGACTGC ACACCAACAT TGACTTCCTC CTTAGCCTGT CTGGCCACCC GGAATTTGAA 
GCTGGGAACG TGCACACTGA TTTCATCGCT CAACACCGCA AAGAACTGTT GCCCGACCGG 
AAGACCACAG CCAAGGAGTT TTTATGTCAC GCAGCTCTGG CCCTCATCCT CAAGGAGAAA 
GCTGTGTCTG ATGTCTTCAA AATTCAGACA CAAGATCAAT ACTCTCCTTT TGCATCCAGC 
AGTGGAAGAA GACTGAATAT CTGTTACTCT AGAAACATGA CTCTTAGGGA TGGTAAAAAC 
AATGTAGCCA TAGCTGTAAC ATATAACCGT GATGGATCAT ATAGCATGCA GATTGAAGAT 
AAAACTTTCC ATGTCCTTGG TGATCTTTGC AGTGAGGGAG ACTGCACTTA TCTGAAATCT 
TCTGTTAATG GAGTTGCTAG TAAAATTAAG CTAATTATCC TGAAAAACAC TATTTACCTA 
TTTTCCATGG ATGGAAGTAT TCAGATTGGC ATTCCGGTTC CCCGATACTT GTCTTCAGCA 
AGCTCAGGAG AAACCCAAGG GGGTGCTGTA GCTCCTATGA CTGGAACTGT TGAAAAGGTG 
TTTGTGAAAG CTGGAGACAG AGTAAAAGCT GGAGACTCTC TGATGGTTAT GATTGCCATG 
AAAATGGAAC ATACCATAAA GGCGCCAAAG GATGGCACAA TCAAGAAGGT ATTCTACAAA 
GAAGGTTCTC AGGCCAACAG ACACGCTCCT TTAGTCGAGT TTGAGGAGGA AGACTCTGAC 
GAAAGGGAAG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192687.1
CDS	96..2273
Translation	MSAASLVSVLLVAAERHRWQRVPRLLLPPRGWAWKQRTRKYATTTGQNISKVLIANRGEIACRVIRTAKKMGVQSVAVYSEADRNSMHVDMADEAYLIGPAPSQQSYLSMEKIIQVAKISAAQAIHPGYGFLSENMEFAELCKQEGIIFVGPPSSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARTIGYPVMIKAVRGGGGKGMRIVRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPGPGIKPEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLRGHAFEARIYAEDPNNNFMPGAGPLVHLSTPRADLTTRIETGVRQGDEVSMHYDPMIAKLVVWAADRQAALTKLKYSLRQYNIVGLHTNIDFLLSLSGHPEFEAGNVHTDFIAQHRKELLPDRKTTAKEFLCHAALALILKEKAVSDVFKIQTQDQYSPFASSSGRRLNICYSRNMTLRDGKNNVAIAVTYNRDGSYSMQIEDKTFHVLGDLCSEGDCTYLKSSVNGVASKIKLIILKNTIYLFSMDGSIQIGIPVPRYLSSASSGETQGGAVAPMTGTVEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGTIKKVFYKEGSQANRHAPLVEFEEEDSDEREAD

Target ORF information:

RefSeq Version	NM_001205758.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205758.1

ORF Insert Sequence:

ATGTCGGCGG CCTCTTTGGT GTCGGTGCTG CTCGTGGCGG CGGAGAGGCA CCGGTGGCAG 
CGAGTCCCGC GCTTGCTGCT GCCGCCGAGG GGATGGGCTT GGAAGCAAAG AACAAGGAAG 
TATGCAACAA CCACAGGACA AAACATTTCC AAGGTCCTCA TTGCAAACAG AGGAGAAATT 
GCCTGCAGGG TGATACGGAC AGCCAAAAAA ATGGGTGTAC AGTCTGTGGC TGTTTATAGT 
GAGGCCGACA GGAATTCCAT GCATGTTGAC ATGGCAGATG AAGCCTATCT CATCGGCCCC 
GCTCCCTCTC AGCAGAGCTA CCTTTCTATG GAAAAAATCA TTCAAGTGGC CAAGATCTCT 
GCCGCACAGG CTATCCATCC AGGATATGGC TTTCTCTCAG AAAACATGGA ATTTGCCGAA 
CTGTGTAAGC AAGAAGGAAT TATTTTTGTA GGTCCTCCTT CATCAGCAAT TAGAGACATG 
GGTATAAAGA GCACATCCAA ATCCATCATG GCTGCTGCTG GAGTGCCTGT GGTGGAGGGT 
TATCACGGGG AGGACCAGTC AGACCAATGC CTGAAAGAGC ACGCTAGGAC AATCGGTTAC 
CCCGTCATGA TTAAAGCCGT CCGTGGCGGA GGAGGAAAAG GTATGAGGAT CGTTAGATCT 
GAAAAAGAAT TTCAAGAGCA GTTAGAATCA GCACGGAGAG AAGCTAAGAA GTCTTTCAAT 
GATGATGCCA TGCTGATTGA GAAGTTTGTG GACACACCAA GGCATGTAGA AGTGCAGGTG 
TTTGGTGACC ACCATGGCAA TGCCGTGTAC TTGTTTGAAA GAGACTGTAG TGTACAGAGA 
AGACATCAGA AGATCATTGA AGAGGCCCCA GGGCCTGGTA TTAAACCTGA AGTAAGAAAA 
AAGCTTGGAG AAGCTGCAGT CAGAGCTGCT AAAGCTGTAA ATTATGTTGG TGCAGGAACC 
GTGGAGTTTA TCATGGACTC AAAGCACAAT TTCTACTTTA TGGAGATGAA TACAAGGCTG 
CAAGTGGAAC ATCCTGTCAC CGAGATGATC ACAGGAACTG ACTTGGTGGA ATGGCAACTT 
AGGATTGCAG CAGGAGAGAA GATTCCTTTG AGCCAGGAAG AAATAACTCT GCGGGGCCAT 
GCCTTTGAAG CTAGAATATA TGCAGAAGAT CCTAACAATA ACTTCATGCC GGGGGCCGGA 
CCACTAGTGC ATCTCTCCAC CCCTCGAGCA GACCTTACCA CTAGGATTGA AACTGGAGTA 
CGGCAAGGAG ATGAAGTTTC AATGCATTAT GACCCCATGA TTGCAAAGCT GGTTGTGTGG 
GCTGCTGATC GCCAGGCAGC CTTGACGAAA CTTAAGTACA GCCTTCGACA GTACAATATT 
GTTGGACTGC ACACCAACAT TGACTTCCTC CTTAGCCTGT CTGGCCACCC GGAATTTGAA 
GCTGGGAACG TGCACACTGA TTTCATCGCT CAACACCGCA AAGAACTGTT GCCCGACCGG 
AAGACCACAG CCAAGGAGTT TTTATGTCAC GCAGCTCTGG CCCTCATCCT CAAGGAGAAA 
GCTGTGTCTG ATGTCTTCAA AATTCAGACA CAAGATCAAT ACTCTCCTTT TGCATCCAGC 
AGTGGAAGAA GACTGAATAT CTGTTACTCT AGAAACATGA CTCTTAGGGA TGGTAAAAAC 
AATGTAGCCA TAGCTGTAAC ATATAACCGT GATGGATCAT ATAGCATGCA GATTGAAGAT 
AAAACTTTCC ATGTCCTTGG TGATCTTTGC AGTGAGGGAG ACTGCACTTA TCTGAAATCT 
TCTGTTAATG GAGTTGCTAG TAAAATTAAG CTAATTATCC TGAAAAACAC TATTTACCTA 
TTTTCCATGG ATGGAAGTAT TCAGATTGGC ATTCCGGTTC CCCGATACTT GTCTTCAGCA 
AGCTCAGGAG AAACCCAAGG GGGTGCTGTA GCTCCTATGA CTGGAACTGT TGAAAAGGTG 
TTTGTGAAAG CTGGAGACAG AGTAAAAGCT GGAGACTCTC TGATGGTTAT GATTGCCATG 
AAAATGGAAC ATACCATAAA GGCGCCAAAG GATGGCACAA TCAAGAAGGT ATTCTACAAA 
GAAGGTTCTC AGGCCAACAG ACACGCTCCT TTAGTCGAGT TTGAGGAGGA AGACTCTGAC 
GAAAGGGAAG CAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC1 ( XM_015472184.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327670.1 Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X2, mRNA.

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CloneID	OBa87759
Clone ID Related Accession (Same CDS sequence)	XM_015472184.1 , XM_015472184.2
Accession Version	XM_015472184.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAA TCATTCAAGT GGCCAAGATC TCTGCCGCAC AGGCTATCCA TCCAGGATAT 
GGCTTTCTCT CAGAAAACAT GGAATTTGCC GAACTGTGTA AGCAAGAAGG AATTATTTTT 
GTAGGTCCTC CTTCATCAGC AATTAGAGAC ATGGGTATAA AGAGCACATC CAAATCCATC 
ATGGCTGCTG CTGGAGTGCC TGTGGTGGAG GGTTATCACG GGGAGGACCA GTCAGACCAA 
TGCCTGAAAG AGCACGCTAG GACAATCGGT TACCCCGTCA TGATTAAAGC CGTCCGTGGC 
GGAGGAGGAA AAGGTATGAG GATCGTTAGA TCTGAAAAAG AATTTCAAGA GCAGTTAGAA 
TCAGCACGGA GAGAAGCTAA GAAGTCTTTC AATGATGATG CCATGCTGAT TGAGAAGTTT 
GTGGACACAC CAAGGCATGT AGAAGTGCAG GTGTTTGGTG ACCACCATGG CAATGCCGTG 
TACTTGTTTG AAAGAGACTG TAGTGTACAG AGAAGACATC AGAAGATCAT TGAAGAGGCC 
CCAGGGCCTG GTATTAAACC TGAAGTAAGA AAAAAGCTTG GAGAAGCTGC AGTCAGAGCT 
GCTAAAGCTG TAAATTATGT TGGTGCAGGA ACCGTGGAGT TTATCATGGA CTCAAAGCAC 
AATTTCTACT TTATGGAGAT GAATACAAGG CTGCAAGTGG AACATCCTGT CACCGAGATG 
ATCACAGGAA CTGACTTGGT GGAATGGCAA CTTAGGATTG CAGCAGGAGA GAAGATTCCT 
TTGAGCCAGG AAGAAATAAC TCTGCGGGGC CATGCCTTTG AAGCTAGAAT ATATGCAGAA 
GATCCTAACA ATAACTTCAT GCCGGGGGCC GGACCACTAG TGCATCTCTC CACCCCTCGA 
GCAGACCTTA CCACTAGGAT TGAAACTGGA GTACGGCAAG GAGATGAAGT TTCAATGCAT 
TATGACCCCA TGATTGCAAA GCTGGTTGTG TGGGCTGCTG ATCGCCAGGC AGCCTTGACG 
AAACTTAAGT ACAGCCTTCG ACAGTACAAT ATTGTTGGAC TGCACACCAA CATTGACTTC 
CTCCTTAGCC TGTCTGGCCA CCCGGAATTT GAAGCTGGGA ACGTGCACAC TGATTTCATC 
GCTCAACACC GCAAAGAACT GTTGCCCGAC CGGAAGACCA CAGCCAAGGA GTTTTTATGT 
CACGCAGCTC TGGCCCTCAT CCTCAAGGAG AAAGCTGTGT CTGATGTCTT CAAAATTCAG 
ACACAAGATC AATACTCTCC TTTTGCATCC AGCAGTGGAA GAAGACTGAA TATCTGTTAC 
TCTAGAAACA TGACTCTTAG GGATGGTAAA AACAATGTAG CCATAGCTGT AACATATAAC 
CGTGATGGAT CATATAGCAT GCAGATTGAA GATAAAACTT TCCATGTCCT TGGTGATCTT 
TGCAGTGAGG GAGACTGCAC TTATCTGAAA TCTTCTGTTA ATGGAGTTGC TAGTAAAATT 
AAGCTAATTA TCCTGAAAAA CACTATTTAC CTATTTTCCA TGGATGGAAG TATTCAGATT 
GGCATTCCGG TTCCCCGATA CTTGTCTTCA GCAAGCTCAG GAGAAACCCA AGGGGGTGCT 
GTAGCTCCTA TGACTGGAAC TGTTGAAAAG GTGTTTGTGA AAGCTGGAGA CAGAGTAAAA 
GCTGGAGACT CTCTGATGGT TATGATTGCC ATGAAAATGG AACATACCAT AAAGGCGCCA 
AAGGATGGCA CAATCAAGAA GGTATTCTAC AAAGAAGGTT CTCAGGCCAA CAGACACGCT 
CCTTTAGTCG AGTTTGAGGA GGAAGACTCT GACGAAAGGG AAGCAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327670.1
CDS	241..2091
Translation	MEKIIQVAKISAAQAIHPGYGFLSENMEFAELCKQEGIIFVGPPSSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARTIGYPVMIKAVRGGGGKGMRIVRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPGPGIKPEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLRGHAFEARIYAEDPNNNFMPGAGPLVHLSTPRADLTTRIETGVRQGDEVSMHYDPMIAKLVVWAADRQAALTKLKYSLRQYNIVGLHTNIDFLLSLSGHPEFEAGNVHTDFIAQHRKELLPDRKTTAKEFLCHAALALILKEKAVSDVFKIQTQDQYSPFASSSGRRLNICYSRNMTLRDGKNNVAIAVTYNRDGSYSMQIEDKTFHVLGDLCSEGDCTYLKSSVNGVASKIKLIILKNTIYLFSMDGSIQIGIPVPRYLSSASSGETQGGAVAPMTGTVEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGTIKKVFYKEGSQANRHAPLVEFEEEDSDEREAD

Target ORF information:

RefSeq Version	XM_015472184.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472184.1

ORF Insert Sequence:

ATGGAAAAAA TCATTCAAGT GGCCAAGATC TCTGCCGCAC AGGCTATCCA TCCAGGATAT 
GGCTTTCTCT CAGAAAACAT GGAATTTGCC GAACTGTGTA AGCAAGAAGG AATTATTTTT 
GTAGGTCCTC CTTCATCAGC AATTAGAGAC ATGGGTATAA AGAGCACATC CAAATCCATC 
ATGGCTGCTG CTGGAGTGCC TGTGGTGGAG GGTTATCACG GGGAGGACCA GTCAGACCAA 
TGCCTGAAAG AGCACGCTAG GACAATCGGT TACCCCGTCA TGATTAAAGC CGTCCGTGGC 
GGAGGAGGAA AAGGTATGAG GATCGTTAGA TCTGAAAAAG AATTTCAAGA GCAGTTAGAA 
TCAGCACGGA GAGAAGCTAA GAAGTCTTTC AATGATGATG CCATGCTGAT TGAGAAGTTT 
GTGGACACAC CAAGGCATGT AGAAGTGCAG GTGTTTGGTG ACCACCATGG CAATGCCGTG 
TACTTGTTTG AAAGAGACTG TAGTGTACAG AGAAGACATC AGAAGATCAT TGAAGAGGCC 
CCAGGGCCTG GTATTAAACC TGAAGTAAGA AAAAAGCTTG GAGAAGCTGC AGTCAGAGCT 
GCTAAAGCTG TAAATTATGT TGGTGCAGGA ACCGTGGAGT TTATCATGGA CTCAAAGCAC 
AATTTCTACT TTATGGAGAT GAATACAAGG CTGCAAGTGG AACATCCTGT CACCGAGATG 
ATCACAGGAA CTGACTTGGT GGAATGGCAA CTTAGGATTG CAGCAGGAGA GAAGATTCCT 
TTGAGCCAGG AAGAAATAAC TCTGCGGGGC CATGCCTTTG AAGCTAGAAT ATATGCAGAA 
GATCCTAACA ATAACTTCAT GCCGGGGGCC GGACCACTAG TGCATCTCTC CACCCCTCGA 
GCAGACCTTA CCACTAGGAT TGAAACTGGA GTACGGCAAG GAGATGAAGT TTCAATGCAT 
TATGACCCCA TGATTGCAAA GCTGGTTGTG TGGGCTGCTG ATCGCCAGGC AGCCTTGACG 
AAACTTAAGT ACAGCCTTCG ACAGTACAAT ATTGTTGGAC TGCACACCAA CATTGACTTC 
CTCCTTAGCC TGTCTGGCCA CCCGGAATTT GAAGCTGGGA ACGTGCACAC TGATTTCATC 
GCTCAACACC GCAAAGAACT GTTGCCCGAC CGGAAGACCA CAGCCAAGGA GTTTTTATGT 
CACGCAGCTC TGGCCCTCAT CCTCAAGGAG AAAGCTGTGT CTGATGTCTT CAAAATTCAG 
ACACAAGATC AATACTCTCC TTTTGCATCC AGCAGTGGAA GAAGACTGAA TATCTGTTAC 
TCTAGAAACA TGACTCTTAG GGATGGTAAA AACAATGTAG CCATAGCTGT AACATATAAC 
CGTGATGGAT CATATAGCAT GCAGATTGAA GATAAAACTT TCCATGTCCT TGGTGATCTT 
TGCAGTGAGG GAGACTGCAC TTATCTGAAA TCTTCTGTTA ATGGAGTTGC TAGTAAAATT 
AAGCTAATTA TCCTGAAAAA CACTATTTAC CTATTTTCCA TGGATGGAAG TATTCAGATT 
GGCATTCCGG TTCCCCGATA CTTGTCTTCA GCAAGCTCAG GAGAAACCCA AGGGGGTGCT 
GTAGCTCCTA TGACTGGAAC TGTTGAAAAG GTGTTTGTGA AAGCTGGAGA CAGAGTAAAA 
GCTGGAGACT CTCTGATGGT TATGATTGCC ATGAAAATGG AACATACCAT AAAGGCGCCA 
AAGGATGGCA CAATCAAGAA GGTATTCTAC AAAGAAGGTT CTCAGGCCAA CAGACACGCT 
CCTTTAGTCG AGTTTGAGGA GGAAGACTCT GACGAAAGGG AAGCAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC1 ( XM_015472184.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327670.1 Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X2, mRNA.

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CloneID	OBa87759
Clone ID Related Accession (Same CDS sequence)	XM_015472184.1 , XM_015472184.2
Accession Version	XM_015472184.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAA TCATTCAAGT GGCCAAGATC TCTGCCGCAC AGGCTATCCA TCCAGGATAT 
GGCTTTCTCT CAGAAAACAT GGAATTTGCC GAACTGTGTA AGCAAGAAGG AATTATTTTT 
GTAGGTCCTC CTTCATCAGC AATTAGAGAC ATGGGTATAA AGAGCACATC CAAATCCATC 
ATGGCTGCTG CTGGAGTGCC TGTGGTGGAG GGTTATCACG GGGAGGACCA GTCAGACCAA 
TGCCTGAAAG AGCACGCTAG GACAATCGGT TACCCCGTCA TGATTAAAGC CGTCCGTGGC 
GGAGGAGGAA AAGGTATGAG GATCGTTAGA TCTGAAAAAG AATTTCAAGA GCAGTTAGAA 
TCAGCACGGA GAGAAGCTAA GAAGTCTTTC AATGATGATG CCATGCTGAT TGAGAAGTTT 
GTGGACACAC CAAGGCATGT AGAAGTGCAG GTGTTTGGTG ACCACCATGG CAATGCCGTG 
TACTTGTTTG AAAGAGACTG TAGTGTACAG AGAAGACATC AGAAGATCAT TGAAGAGGCC 
CCAGGGCCTG GTATTAAACC TGAAGTAAGA AAAAAGCTTG GAGAAGCTGC AGTCAGAGCT 
GCTAAAGCTG TAAATTATGT TGGTGCAGGA ACCGTGGAGT TTATCATGGA CTCAAAGCAC 
AATTTCTACT TTATGGAGAT GAATACAAGG CTGCAAGTGG AACATCCTGT CACCGAGATG 
ATCACAGGAA CTGACTTGGT GGAATGGCAA CTTAGGATTG CAGCAGGAGA GAAGATTCCT 
TTGAGCCAGG AAGAAATAAC TCTGCGGGGC CATGCCTTTG AAGCTAGAAT ATATGCAGAA 
GATCCTAACA ATAACTTCAT GCCGGGGGCC GGACCACTAG TGCATCTCTC CACCCCTCGA 
GCAGACCTTA CCACTAGGAT TGAAACTGGA GTACGGCAAG GAGATGAAGT TTCAATGCAT 
TATGACCCCA TGATTGCAAA GCTGGTTGTG TGGGCTGCTG ATCGCCAGGC AGCCTTGACG 
AAACTTAAGT ACAGCCTTCG ACAGTACAAT ATTGTTGGAC TGCACACCAA CATTGACTTC 
CTCCTTAGCC TGTCTGGCCA CCCGGAATTT GAAGCTGGGA ACGTGCACAC TGATTTCATC 
GCTCAACACC GCAAAGAACT GTTGCCCGAC CGGAAGACCA CAGCCAAGGA GTTTTTATGT 
CACGCAGCTC TGGCCCTCAT CCTCAAGGAG AAAGCTGTGT CTGATGTCTT CAAAATTCAG 
ACACAAGATC AATACTCTCC TTTTGCATCC AGCAGTGGAA GAAGACTGAA TATCTGTTAC 
TCTAGAAACA TGACTCTTAG GGATGGTAAA AACAATGTAG CCATAGCTGT AACATATAAC 
CGTGATGGAT CATATAGCAT GCAGATTGAA GATAAAACTT TCCATGTCCT TGGTGATCTT 
TGCAGTGAGG GAGACTGCAC TTATCTGAAA TCTTCTGTTA ATGGAGTTGC TAGTAAAATT 
AAGCTAATTA TCCTGAAAAA CACTATTTAC CTATTTTCCA TGGATGGAAG TATTCAGATT 
GGCATTCCGG TTCCCCGATA CTTGTCTTCA GCAAGCTCAG GAGAAACCCA AGGGGGTGCT 
GTAGCTCCTA TGACTGGAAC TGTTGAAAAG GTGTTTGTGA AAGCTGGAGA CAGAGTAAAA 
GCTGGAGACT CTCTGATGGT TATGATTGCC ATGAAAATGG AACATACCAT AAAGGCGCCA 
AAGGATGGCA CAATCAAGAA GGTATTCTAC AAAGAAGGTT CTCAGGCCAA CAGACACGCT 
CCTTTAGTCG AGTTTGAGGA GGAAGACTCT GACGAAAGGG AAGCAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327670.1
CDS	253..2103
Translation	MEKIIQVAKISAAQAIHPGYGFLSENMEFAELCKQEGIIFVGPPSSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARTIGYPVMIKAVRGGGGKGMRIVRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPGPGIKPEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLRGHAFEARIYAEDPNNNFMPGAGPLVHLSTPRADLTTRIETGVRQGDEVSMHYDPMIAKLVVWAADRQAALTKLKYSLRQYNIVGLHTNIDFLLSLSGHPEFEAGNVHTDFIAQHRKELLPDRKTTAKEFLCHAALALILKEKAVSDVFKIQTQDQYSPFASSSGRRLNICYSRNMTLRDGKNNVAIAVTYNRDGSYSMQIEDKTFHVLGDLCSEGDCTYLKSSVNGVASKIKLIILKNTIYLFSMDGSIQIGIPVPRYLSSASSGETQGGAVAPMTGTVEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGTIKKVFYKEGSQANRHAPLVEFEEEDSDEREAD

Target ORF information:

RefSeq Version	XM_015472184.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methylcrotonoyl-CoA carboxylase 1 (MCCC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472184.2

ORF Insert Sequence:

ATGGAAAAAA TCATTCAAGT GGCCAAGATC TCTGCCGCAC AGGCTATCCA TCCAGGATAT 
GGCTTTCTCT CAGAAAACAT GGAATTTGCC GAACTGTGTA AGCAAGAAGG AATTATTTTT 
GTAGGTCCTC CTTCATCAGC AATTAGAGAC ATGGGTATAA AGAGCACATC CAAATCCATC 
ATGGCTGCTG CTGGAGTGCC TGTGGTGGAG GGTTATCACG GGGAGGACCA GTCAGACCAA 
TGCCTGAAAG AGCACGCTAG GACAATCGGT TACCCCGTCA TGATTAAAGC CGTCCGTGGC 
GGAGGAGGAA AAGGTATGAG GATCGTTAGA TCTGAAAAAG AATTTCAAGA GCAGTTAGAA 
TCAGCACGGA GAGAAGCTAA GAAGTCTTTC AATGATGATG CCATGCTGAT TGAGAAGTTT 
GTGGACACAC CAAGGCATGT AGAAGTGCAG GTGTTTGGTG ACCACCATGG CAATGCCGTG 
TACTTGTTTG AAAGAGACTG TAGTGTACAG AGAAGACATC AGAAGATCAT TGAAGAGGCC 
CCAGGGCCTG GTATTAAACC TGAAGTAAGA AAAAAGCTTG GAGAAGCTGC AGTCAGAGCT 
GCTAAAGCTG TAAATTATGT TGGTGCAGGA ACCGTGGAGT TTATCATGGA CTCAAAGCAC 
AATTTCTACT TTATGGAGAT GAATACAAGG CTGCAAGTGG AACATCCTGT CACCGAGATG 
ATCACAGGAA CTGACTTGGT GGAATGGCAA CTTAGGATTG CAGCAGGAGA GAAGATTCCT 
TTGAGCCAGG AAGAAATAAC TCTGCGGGGC CATGCCTTTG AAGCTAGAAT ATATGCAGAA 
GATCCTAACA ATAACTTCAT GCCGGGGGCC GGACCACTAG TGCATCTCTC CACCCCTCGA 
GCAGACCTTA CCACTAGGAT TGAAACTGGA GTACGGCAAG GAGATGAAGT TTCAATGCAT 
TATGACCCCA TGATTGCAAA GCTGGTTGTG TGGGCTGCTG ATCGCCAGGC AGCCTTGACG 
AAACTTAAGT ACAGCCTTCG ACAGTACAAT ATTGTTGGAC TGCACACCAA CATTGACTTC 
CTCCTTAGCC TGTCTGGCCA CCCGGAATTT GAAGCTGGGA ACGTGCACAC TGATTTCATC 
GCTCAACACC GCAAAGAACT GTTGCCCGAC CGGAAGACCA CAGCCAAGGA GTTTTTATGT 
CACGCAGCTC TGGCCCTCAT CCTCAAGGAG AAAGCTGTGT CTGATGTCTT CAAAATTCAG 
ACACAAGATC AATACTCTCC TTTTGCATCC AGCAGTGGAA GAAGACTGAA TATCTGTTAC 
TCTAGAAACA TGACTCTTAG GGATGGTAAA AACAATGTAG CCATAGCTGT AACATATAAC 
CGTGATGGAT CATATAGCAT GCAGATTGAA GATAAAACTT TCCATGTCCT TGGTGATCTT 
TGCAGTGAGG GAGACTGCAC TTATCTGAAA TCTTCTGTTA ATGGAGTTGC TAGTAAAATT 
AAGCTAATTA TCCTGAAAAA CACTATTTAC CTATTTTCCA TGGATGGAAG TATTCAGATT 
GGCATTCCGG TTCCCCGATA CTTGTCTTCA GCAAGCTCAG GAGAAACCCA AGGGGGTGCT 
GTAGCTCCTA TGACTGGAAC TGTTGAAAAG GTGTTTGTGA AAGCTGGAGA CAGAGTAAAA 
GCTGGAGACT CTCTGATGGT TATGATTGCC ATGAAAATGG AACATACCAT AAAGGCGCCA 
AAGGATGGCA CAATCAAGAA GGTATTCTAC AAAGAAGGTT CTCAGGCCAA CAGACACGCT 
CCTTTAGTCG AGTTTGAGGA GGAAGACTCT GACGAAAGGG AAGCAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MCCC1 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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