Spry4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spry4
Entrez Gene ID	291610
Full Name	sprouty RTK signaling antagonist 4
General protein information	Preferred Names sprouty RTK signaling antagonist 4 Names protein sprouty homolog 4 sprouty homolog 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106150.1	NP_001099620.1	protein sprouty homolog 4
XM_017600894.1	XP_017456383.1	protein sprouty homolog 4 isoform X1

Pathways from BioSystems

Homologies

	SPRY4	XP_001095585.2
Rattus norvegicus (Norway rat)	Spry4	NP_001099620.1
Gallus gallus (chicken)	SPRY4	NP_001073203.1
Danio rerio (zebrafish)	spry4	NP_571901.1
Bos taurus (cattle)	SPRY4	NP_001074981.1
Homo sapiens (human)	SPRY4	NP_112226.2
Canis lupus familiaris (dog)	SPRY4	XP_544320.2
Mus musculus (house mouse)	Spry4	NP_036028.2
Pan troglodytes (chimpanzee)	SPRY4	XP_527058.2
Gallus gallus (chicken)	LOC100858637	XP_004945094.1
Xenopus tropicalis (tropical clawed frog)	spry4	XP_002939581.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of Sproutys and SPREDs is decreased during lung branching morphogenesis in nitrofen-induced pulmonary hypoplasia.
Friedmacher F, Gosemann JH, Fujiwara N, Takahashi H, Hofmann A, Puri P
Pediatric surgery international29(11)1193-8(2013 Nov)

Sprouty4 is an endogenous negative modulator of TrkA signaling and neuronal differentiation induced by NGF.
Alsina FC, Irala D, Fontanet PA, Hita FJ, Ledda F, Paratcha G
PloS one7(2)e32087(2012)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)

Efficient suppression of FGF-2-induced ERK activation by the cooperative interaction among mammalian Sprouty isoforms.
Ozaki K, Miyazaki S, Tanimura S, Kohno M
Journal of cell science118(Pt 24)5861-71(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Decreased expression of Sproutys and SPREDs during the terminal pseudoglandular stage may disrupt lung branching morphogenesis by interfering with epithelial-mesenchymal interactions contributing to PH.
Title: Expression of Sproutys and SPREDs is decreased during lung branching morphogenesis in nitrofen-induced pulmonary hypoplasia.

Spry4 regulates neurite outgrowth reducing not only the MAPK pathway but also restricting TrkA signaling and Rac1 activation in response to NGF
Title: Sprouty4 is an endogenous negative modulator of TrkA signaling and neuronal differentiation induced by NGF.

The following Spry4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spry4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00270	NM_001106150.1 Latest version!	Rattus norvegicus sprouty RTK signaling antagonist 4 (Spry4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa00270	XM_017600894.1 Latest version!	Rattus norvegicus sprouty RTK signaling antagonist 4 (Spry4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spry4 ( NM_001106150.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099620.1 Rattus norvegicus sprouty RTK signaling antagonist 4 (Spry4), mRNA.

Price & Availability↑

CloneID	ORa00270
Clone ID Related Accession (Same CDS sequence)	NM_001106150.1 , XM_017600894.1
Accession Version	NM_001106150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	protein sprouty homolog 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473974.1. On or before Oct 4, 2007 this sequence version replaced XM_225969.3, XM_001066725.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCC CGATTCCACA GAGCGGCGTC CCTTTGAATC CCAGCTCAGT CATGGTGCAG 
CCCCTCCTTG ACAGCAGAGT GCCCCACAGC CGGCTCCAGC ACCCACTCAC CATCTTGCCC 
ATTGACCAGA TGAAGACCAG CCACGTGGAG AATGACTACA TAGACAACCC TAGCCTGGCC 
CCTGCCACGG GTCCCAAGCG GCCCCGGGGT GGGGCCCCAG AGCTGGCTCC TACACCTGCC 
CGCTGTGACC AGGATGTTAC TCACCACTGG ATCTCCTTCA GCGGTCGGCC CAGCTCTGTA 
AGCAGCAGCA GCAGCACTTC CTCTGACCAG CGGCTCCTGG ATCACATGGC TCCACCACCG 
GTGACTGAGC AGGCCTCCCC CAGGGCTGTT CGCCTCCAGC CCAAGGTGGT CCACTGTAAG 
CCACTGGACC TCAAGGGCCC AGCAGCTCCA CCAGAGCTAG ACAAGCACTT CTTGCTGTGC 
GAGGCCTGTG GGAAGTGTAA GTGCAAGGAG TGTGCGTCCC CTCGGACGTT ACCCTCCTGC 
TGGGTGTGCA ACCAGGAGTG CCTGTGCTCC GCCCAGACCC TGGTCAACTA CAGCACGTGC 
ATGTGCCTGG TGCAAGGTAT CTTCTACCAC TGTACTAACG AGGATGATGA GGGCTCTTGT 
GCTGACCATC CCTGCTCCTG CTCCGGCTCC AACTGCTGCG CCCGCTGGTC CTTCATGGGC 
GCCCTCTCTC TGGTGCTGCC CTGTCTGCTG TGCTACCTGC CAGCCACGGG CTGCGTCAAG 
CTGGCCCAGC GCGGCTATGA CCGCCTGAGA CGTCCCGGTT GCCGCTGCAA GCACACGAAC 
AGCGTCATCT GCAAGGCAGC CAGTGGGGAC ACCAAGGCCA GCAGGTCCGA CAAGCCTTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099620.1
CDS	299..1201
Translation	MEPPIPQSGVPLNPSSVMVQPLLDSRVPHSRLQHPLTILPIDQMKTSHVENDYIDNPSLAPATGPKRPRGGAPELAPTPARCDQDVTHHWISFSGRPSSVSSSSSTSSDQRLLDHMAPPPVTEQASPRAVRLQPKVVHCKPLDLKGPAAPPELDKHFLLCEACGKCKCKECASPRTLPSCWVCNQECLCSAQTLVNYSTCMCLVQGIFYHCTNEDDEGSCADHPCSCSGSNCCARWSFMGALSLVLPCLLCYLPATGCVKLAQRGYDRLRRPGCRCKHTNSVICKAASGDTKASRSDKPF

Target ORF information:

RefSeq Version	NM_001106150.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sprouty RTK signaling antagonist 4 (Spry4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106150.1

ORF Insert Sequence:

ATGGAGCCCC CGATTCCACA GAGCGGCGTC CCTTTGAATC CCAGCTCAGT CATGGTGCAG 
CCCCTCCTTG ACAGCAGAGT GCCCCACAGC CGGCTCCAGC ACCCACTCAC CATCTTGCCC 
ATTGACCAGA TGAAGACCAG CCACGTGGAG AATGACTACA TAGACAACCC TAGCCTGGCC 
CCTGCCACGG GTCCCAAGCG GCCCCGGGGT GGGGCCCCAG AGCTGGCTCC TACACCTGCC 
CGCTGTGACC AGGATGTTAC TCACCACTGG ATCTCCTTCA GCGGTCGGCC CAGCTCTGTA 
AGCAGCAGCA GCAGCACTTC CTCTGACCAG CGGCTCCTGG ATCACATGGC TCCACCACCG 
GTGACTGAGC AGGCCTCCCC CAGGGCTGTT CGCCTCCAGC CCAAGGTGGT CCACTGTAAG 
CCACTGGACC TCAAGGGCCC AGCAGCTCCA CCAGAGCTAG ACAAGCACTT CTTGCTGTGC 
GAGGCCTGTG GGAAGTGTAA GTGCAAGGAG TGTGCGTCCC CTCGGACGTT ACCCTCCTGC 
TGGGTGTGCA ACCAGGAGTG CCTGTGCTCC GCCCAGACCC TGGTCAACTA CAGCACGTGC 
ATGTGCCTGG TGCAAGGTAT CTTCTACCAC TGTACTAACG AGGATGATGA GGGCTCTTGT 
GCTGACCATC CCTGCTCCTG CTCCGGCTCC AACTGCTGCG CCCGCTGGTC CTTCATGGGC 
GCCCTCTCTC TGGTGCTGCC CTGTCTGCTG TGCTACCTGC CAGCCACGGG CTGCGTCAAG 
CTGGCCCAGC GCGGCTATGA CCGCCTGAGA CGTCCCGGTT GCCGCTGCAA GCACACGAAC 
AGCGTCATCT GCAAGGCAGC CAGTGGGGAC ACCAAGGCCA GCAGGTCCGA CAAGCCTTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spry4 ( XM_017600894.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456383.1 Rattus norvegicus sprouty RTK signaling antagonist 4 (Spry4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00270
Clone ID Related Accession (Same CDS sequence)	NM_001106150.1 , XM_017600894.1
Accession Version	XM_017600894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein sprouty homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCC CGATTCCACA GAGCGGCGTC CCTTTGAATC CCAGCTCAGT CATGGTGCAG 
CCCCTCCTTG ACAGCAGAGT GCCCCACAGC CGGCTCCAGC ACCCACTCAC CATCTTGCCC 
ATTGACCAGA TGAAGACCAG CCACGTGGAG AATGACTACA TAGACAACCC TAGCCTGGCC 
CCTGCCACGG GTCCCAAGCG GCCCCGGGGT GGGGCCCCAG AGCTGGCTCC TACACCTGCC 
CGCTGTGACC AGGATGTTAC TCACCACTGG ATCTCCTTCA GCGGTCGGCC CAGCTCTGTA 
AGCAGCAGCA GCAGCACTTC CTCTGACCAG CGGCTCCTGG ATCACATGGC TCCACCACCG 
GTGACTGAGC AGGCCTCCCC CAGGGCTGTT CGCCTCCAGC CCAAGGTGGT CCACTGTAAG 
CCACTGGACC TCAAGGGCCC AGCAGCTCCA CCAGAGCTAG ACAAGCACTT CTTGCTGTGC 
GAGGCCTGTG GGAAGTGTAA GTGCAAGGAG TGTGCGTCCC CTCGGACGTT ACCCTCCTGC 
TGGGTGTGCA ACCAGGAGTG CCTGTGCTCC GCCCAGACCC TGGTCAACTA CAGCACGTGC 
ATGTGCCTGG TGCAAGGTAT CTTCTACCAC TGTACTAACG AGGATGATGA GGGCTCTTGT 
GCTGACCATC CCTGCTCCTG CTCCGGCTCC AACTGCTGCG CCCGCTGGTC CTTCATGGGC 
GCCCTCTCTC TGGTGCTGCC CTGTCTGCTG TGCTACCTGC CAGCCACGGG CTGCGTCAAG 
CTGGCCCAGC GCGGCTATGA CCGCCTGAGA CGTCCCGGTT GCCGCTGCAA GCACACGAAC 
AGCGTCATCT GCAAGGCAGC CAGTGGGGAC ACCAAGGCCA GCAGGTCCGA CAAGCCTTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456383.1
CDS	145..1047
Translation	MEPPIPQSGVPLNPSSVMVQPLLDSRVPHSRLQHPLTILPIDQMKTSHVENDYIDNPSLAPATGPKRPRGGAPELAPTPARCDQDVTHHWISFSGRPSSVSSSSSTSSDQRLLDHMAPPPVTEQASPRAVRLQPKVVHCKPLDLKGPAAPPELDKHFLLCEACGKCKCKECASPRTLPSCWVCNQECLCSAQTLVNYSTCMCLVQGIFYHCTNEDDEGSCADHPCSCSGSNCCARWSFMGALSLVLPCLLCYLPATGCVKLAQRGYDRLRRPGCRCKHTNSVICKAASGDTKASRSDKPF

Target ORF information:

RefSeq Version	XM_017600894.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sprouty RTK signaling antagonist 4 (Spry4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600894.1

ORF Insert Sequence:

ATGGAGCCCC CGATTCCACA GAGCGGCGTC CCTTTGAATC CCAGCTCAGT CATGGTGCAG 
CCCCTCCTTG ACAGCAGAGT GCCCCACAGC CGGCTCCAGC ACCCACTCAC CATCTTGCCC 
ATTGACCAGA TGAAGACCAG CCACGTGGAG AATGACTACA TAGACAACCC TAGCCTGGCC 
CCTGCCACGG GTCCCAAGCG GCCCCGGGGT GGGGCCCCAG AGCTGGCTCC TACACCTGCC 
CGCTGTGACC AGGATGTTAC TCACCACTGG ATCTCCTTCA GCGGTCGGCC CAGCTCTGTA 
AGCAGCAGCA GCAGCACTTC CTCTGACCAG CGGCTCCTGG ATCACATGGC TCCACCACCG 
GTGACTGAGC AGGCCTCCCC CAGGGCTGTT CGCCTCCAGC CCAAGGTGGT CCACTGTAAG 
CCACTGGACC TCAAGGGCCC AGCAGCTCCA CCAGAGCTAG ACAAGCACTT CTTGCTGTGC 
GAGGCCTGTG GGAAGTGTAA GTGCAAGGAG TGTGCGTCCC CTCGGACGTT ACCCTCCTGC 
TGGGTGTGCA ACCAGGAGTG CCTGTGCTCC GCCCAGACCC TGGTCAACTA CAGCACGTGC 
ATGTGCCTGG TGCAAGGTAT CTTCTACCAC TGTACTAACG AGGATGATGA GGGCTCTTGT 
GCTGACCATC CCTGCTCCTG CTCCGGCTCC AACTGCTGCG CCCGCTGGTC CTTCATGGGC 
GCCCTCTCTC TGGTGCTGCC CTGTCTGCTG TGCTACCTGC CAGCCACGGG CTGCGTCAAG 
CTGGCCCAGC GCGGCTATGA CCGCCTGAGA CGTCCCGGTT GCCGCTGCAA GCACACGAAC 
AGCGTCATCT GCAAGGCAGC CAGTGGGGAC ACCAAGGCCA GCAGGTCCGA CAAGCCTTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of Sproutys and SPREDs is decreased during lung branching morphogenesis in nitrofen-induced pulmonary hypoplasia.
Pediatric surgery international29(11)1193-8(2013 Nov)
Friedmacher F,Gosemann JH,Fujiwara N,Takahashi H,Hofmann A,Puri P

Sprouty4 is an endogenous negative modulator of TrkA signaling and neuronal differentiation induced by NGF.
PloS one7(2)e32087(2012)
Alsina FC,Irala D,Fontanet PA,Hita FJ,Ledda F,Paratcha G

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)
Tarantino C,Paolella G,Cozzuto L,Minopoli G,Pastore L,Parisi S,Russo T

Efficient suppression of FGF-2-induced ERK activation by the cooperative interaction among mammalian Sprouty isoforms.
Journal of cell science118(Pt 24)5861-71(2005 Dec)
Ozaki K,Miyazaki S,Tanimura S,Kohno M