Mstn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mstn
Entrez Gene ID	29152
Full Name	myostatin
Synonyms	Gdf8
General protein information	Preferred Names myostatin Names growth/differentiation factor 8 GDF-8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q22
Summary	This gene encodes a member of the TGF-beta (transforming growth factor-beta) family of proteins. The encoded preproprotein is proteolytically processed to generate an N-terminal propeptide and a C-terminal subunit. The C-terminal subunits form the mature homodimer, which negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jun 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019151.1	NP_062024.1	growth/differentiation factor 8 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MSTN	NP_001001461.1
Xenopus tropicalis (tropical clawed frog)	LOC100496408	XP_002931568.1
Homo sapiens (human)	MSTN	NP_005250.1
	MSTN	NP_001073588.1
Pan troglodytes (chimpanzee)	MSTN	NP_001073388.1
Canis lupus familiaris (dog)	MSTN	NP_001002959.1
Bos taurus (cattle)	MSTN	NP_001001525.1
Mus musculus (house mouse)	Mstn	NP_034964.1
Danio rerio (zebrafish)	mstnb	NP_571094.1
Rattus norvegicus (Norway rat)	Mstn	NP_062024.1
Drosophila melanogaster (fruit fly)	myo	NP_726604.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the human myostatin precursor and determinants of growth factor latency.
Cotton TR, Fischer G, Wang X, McCoy JC, Czepnik M, Thompson TB, Hyv?nen M
The EMBO journal(2018 Jan)

Myostatin, a profibrotic factor and the main inhibitor of striated muscle mass, is present in the penile and vascular smooth muscle.
Kovanecz I, Masouminia M, Gelfand R, Vernet D, Rajfer J, Gonzalez-Cadavid NF
International journal of impotence research29(5)194-201(2017 Sep)

Myostatin-like proteins regulate synaptic function and neuronal morphology.
Augustin H, McGourty K, Steinert JR, Cochem? HM, Adcott J, Cabecinha M, Vincent A, Halff EF, Kittler JT, Boucrot E, Partridge L
Development (Cambridge, England)144(13)2445-2455(2017 07)

Structural basis for potency differences between GDF8 and GDF11.
Walker RG, Czepnik M, Goebel EJ, McCoy JC, Vujic A, Cho M, Oh J, Aykul S, Walton KL, Schang G, Bernard DJ, Hinck AP, Harrison CA, Martinez-Hackert E, Wagers AJ, Lee RT, Thompson TB
BMC biology15(1)19(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both myostatin and GDF11 affected synapse formation in isolated rat cortical neuron cultures.
Title: Myostatin-like proteins regulate synaptic function and neuronal morphology.

Mstn knockout rat model provides a novel means to study the role of Mstn in metabolism and Mstn-related muscle hypertrophy
Title: Establishment and phenotypic analysis of an Mstn knockout rat.

Results show that Myostatin and follistatin protein expression is reduced in the long-term hypertrophied myocardium from spontaneously hypertensive rats with heart failure.
Title: Myocardial myostatin in spontaneously hypertensive rats with heart failure.

Myostatin can be used as an early marker of doxorubicin induced cardiotoxicity.
Title: Myostatin as a Marker for Doxorubicin Induced Cardiac Damage.

activation of AMPK plays a key role in increasing the level of FoxO1, FoxO3a, and myostatin in gastrocnemius after exercise-induced muscle damage
Title: Activation of AMP-activated protein kinase induce expression of FoxO1, FoxO3a, and myostatin after exercise-induced muscle damage.

The following Mstn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mstn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03864	NM_019151.1 Latest version!	Rattus norvegicus myostatin (Mstn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mstn ( NM_019151.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062024.1 Rattus norvegicus myostatin (Mstn), mRNA.

Price & Availability↑

CloneID	ORa03864
Clone ID Related Accession (Same CDS sequence)	NM_019151.1
Accession Version	NM_019151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Rattus norvegicus(Norway rat)
Product	growth/differentiation factor 8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF019624.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF019624.1, FQ224469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATTCAAA AACCGCAAAT GTATGTTTAT ATTTACCTGT TTGTGCTGAT TGCTGCTGGC 
CCAGTGGATC TAAATGAGGA CAGTGAGAGA GAGGCGAATG TGGAAAAAGA GGGGCTGTGT 
AATGCGTGTG CGTGGAGACA AAACACAAGG TACTCCAGAA TAGAAGCCAT AAAAATTCAA 
ATCCTCAGTA AACTCCGCCT GGAAACAGCG CCTAACATCA GCAAAGATGC TATAAGACAA 
CTTCTGCCCA GAGCGCCTCC ACTCCGGGAA CTGATCGATC AGTACGACGT CCAGAGGGAT 
GACAGCAGTG ACGGCTCTTT GGAAGATGAC GATTATCACG CTACCACGGA AACAATCATT 
ACCATGCCTA CCGAGTCTGA CTTTCTAATG CAAGCGGATG GAAAGCCCAA ATGTTGCTTT 
TTTAAATTTA GCTCTAAAAT ACAGTACAAC AAAGTGGTAA AGGCCCAGCT GTGGATATAT 
CTGAGAGCCG TCAAGACTCC TACAACAGTG TTTGTGCAAA TCCTGAGACT CATCAAACCC 
ATGAAAGACG GTACAAGGTA TACCGGAATC CGATCTCTGA AACTTGACAT GAGCCCAGGC 
ACTGGTATTT GGCAGAGTAT TGATGTGAAG ACAGTGTTGC AAAATTGGCT CAAACAGCCT 
GAATCCAACT TAGGCATTGA AATCAAAGCT TTGGATGAGA ATGGGCATGA TCTTGCTGTA 
ACCTTCCCAG GACCAGGAGA AGATGGGCTG AATCCCTTTT TAGAAGTCAA AGTAACAGAC 
ACACCCAAGA GGTCCCGGAG AGACTTTGGG CTTGACTGTG ATGAACACTC CACGGAATCG 
CGGTGCTGTC GCTACCCCCT CACGGTCGAT TTCGAAGCCT TTGGATGGGA CTGGATTATT 
GCACCCAAAA GATATAAGGC TAATTACTGC TCTGGAGAGT GTGAATTTGT GTTCTTACAA 
AAATATCCGC ATACTCATCT TGTGCACCAA GCAAACCCCA GAGGCTCGGC AGGCCCTTGC 
TGCACGCCAA CAAAAATGTC TCCCATTAAT ATGCTATATT TTAATGGCAA AGAACAAATA 
ATATATGGGA AAATTCCAGC CATGGTAGTA GACCGGTGTG GGTGCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062024.1
CDS	1..1131
Translation	MIQKPQMYVYIYLFVLIAAGPVDLNEDSEREANVEKEGLCNACAWRQNTRYSRIEAIKIQILSKLRLETAPNISKDAIRQLLPRAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQADGKPKCCFFKFSSKIQYNKVVKAQLWIYLRAVKTPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMSPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS

Target ORF information:

RefSeq Version	NM_019151.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myostatin (Mstn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019151.1

ORF Insert Sequence:

ATGATTCAAA AACCGCAAAT GTATGTTTAT ATTTACCTGT TTGTGCTGAT TGCTGCTGGC 
CCAGTGGATC TAAATGAGGA CAGTGAGAGA GAGGCGAATG TGGAAAAAGA GGGGCTGTGT 
AATGCGTGTG CGTGGAGACA AAACACAAGG TACTCCAGAA TAGAAGCCAT AAAAATTCAA 
ATCCTCAGTA AACTCCGCCT GGAAACAGCG CCTAACATCA GCAAAGATGC TATAAGACAA 
CTTCTGCCCA GAGCGCCTCC ACTCCGGGAA CTGATCGATC AGTACGACGT CCAGAGGGAT 
GACAGCAGTG ACGGCTCTTT GGAAGATGAC GATTATCACG CTACCACGGA AACAATCATT 
ACCATGCCTA CCGAGTCTGA CTTTCTAATG CAAGCGGATG GAAAGCCCAA ATGTTGCTTT 
TTTAAATTTA GCTCTAAAAT ACAGTACAAC AAAGTGGTAA AGGCCCAGCT GTGGATATAT 
CTGAGAGCCG TCAAGACTCC TACAACAGTG TTTGTGCAAA TCCTGAGACT CATCAAACCC 
ATGAAAGACG GTACAAGGTA TACCGGAATC CGATCTCTGA AACTTGACAT GAGCCCAGGC 
ACTGGTATTT GGCAGAGTAT TGATGTGAAG ACAGTGTTGC AAAATTGGCT CAAACAGCCT 
GAATCCAACT TAGGCATTGA AATCAAAGCT TTGGATGAGA ATGGGCATGA TCTTGCTGTA 
ACCTTCCCAG GACCAGGAGA AGATGGGCTG AATCCCTTTT TAGAAGTCAA AGTAACAGAC 
ACACCCAAGA GGTCCCGGAG AGACTTTGGG CTTGACTGTG ATGAACACTC CACGGAATCG 
CGGTGCTGTC GCTACCCCCT CACGGTCGAT TTCGAAGCCT TTGGATGGGA CTGGATTATT 
GCACCCAAAA GATATAAGGC TAATTACTGC TCTGGAGAGT GTGAATTTGT GTTCTTACAA 
AAATATCCGC ATACTCATCT TGTGCACCAA GCAAACCCCA GAGGCTCGGC AGGCCCTTGC 
TGCACGCCAA CAAAAATGTC TCCCATTAAT ATGCTATATT TTAATGGCAA AGAACAAATA 
ATATATGGGA AAATTCCAGC CATGGTAGTA GACCGGTGTG GGTGCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of the human myostatin precursor and determinants of growth factor latency.
The EMBO journal(2018 Jan)
Cotton TR,Fischer G,Wang X,McCoy JC,Czepnik M,Thompson TB,Hyv?nen M

Myostatin, a profibrotic factor and the main inhibitor of striated muscle mass, is present in the penile and vascular smooth muscle.
International journal of impotence research29(5)194-201(2017 Sep)
Kovanecz I,Masouminia M,Gelfand R,Vernet D,Rajfer J,Gonzalez-Cadavid NF

Myostatin-like proteins regulate synaptic function and neuronal morphology.
Development (Cambridge, England)144(13)2445-2455(2017 07)
Augustin H,McGourty K,Steinert JR,Cochem? HM,Adcott J,Cabecinha M,Vincent A,Halff EF,Kittler JT,Boucrot E,Partridge L

Structural basis for potency differences between GDF8 and GDF11.
BMC biology15(1)19(2017 Mar)
Walker RG,Czepnik M,Goebel EJ,McCoy JC,Vujic A,Cho M,Oh J,Aykul S,Walton KL,Schang G,Bernard DJ,Hinck AP,Harrison CA,Martinez-Hackert E,Wagers AJ,Lee RT,Thompson TB

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Mstn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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