MSTN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MSTN
Entrez Gene ID	281187
Full Name	myostatin
Synonyms	GDF8
General protein information	Preferred Names myostatin Names growth/differentiation factor 8 GDF-8
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 2 Map Location: 2q14-q15
Summary	This gene encodes a member of the TGF-beta (transforming growth factor-beta) family of proteins. The encoded preproprotein is proteolytically processed to generate an N-terminal propeptide and a C-terminal subunit. The C-terminal subunits form the mature homodimer, which negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jun 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001525.3	NP_001001525.1	growth/differentiation factor 8 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MSTN	NP_001001461.1
Xenopus tropicalis (tropical clawed frog)	LOC100496408	XP_002931568.1
Homo sapiens (human)	MSTN	NP_005250.1
	MSTN	NP_001073588.1
Pan troglodytes (chimpanzee)	MSTN	NP_001073388.1
Canis lupus familiaris (dog)	MSTN	NP_001002959.1
Bos taurus (cattle)	MSTN	NP_001001525.1
Mus musculus (house mouse)	Mstn	NP_034964.1
Danio rerio (zebrafish)	mstnb	NP_571094.1
Rattus norvegicus (Norway rat)	Mstn	NP_062024.1
Drosophila melanogaster (fruit fly)	myo	NP_726604.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of Genetic Polymorphisms in GH/Hpa II and MSTN / Dra I Loci on Body Weight in Friesian Bull Calves.
Nasr SM, Ateya AI, Sadek KM, Radwan HA
Pakistan journal of biological sciences : PJBS19(8-9)338-344(2016)

A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency.
Zhao LH, Zhao YH, Liang H, Yun T, Han XJ, Zhang ML, Zhou X, Hou DX, Li RF, Li XL
Genetics and molecular research : GMR14(1)2750-61(2015 Mar)

Genome edited sheep and cattle.
Proudfoot C, Carlson DF, Huddart R, Long CR, Pryor JH, King TJ, Lillico SG, Mileham AJ, McLaren DG, Whitelaw CB, Fahrenkrug SC
Transgenic research24(1)147-53(2015 Feb)

Influence of single nucleotide polymorphisms in the myostatin and myogenic factor 5 muscle growth-related genes on the performance traits of Marchigiana beef cattle.
Sarti FM, Lasagna E, Ceccobelli S, Di Lorenzo P, Filippini F, Sbarra F, Giontella A, Pieramati C, Panella F
Journal of animal science92(9)3804-10(2014 Sep)

Efficient generation of myostatin (MSTN) biallelic mutations in cattle using zinc finger nucleases.
Luo J, Song Z, Yu S, Cui D, Wang B, Ding F, Li S, Dai Y, Li N
PloS one9(4)e95225(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GH/HpaII locus as candidate marker for body weight in cattle rather than MSTN/DraI.
Title: Effect of Genetic Polymorphisms in GH/Hpa II and MSTN / Dra I Loci on Body Weight in Friesian Bull Calves.

Data indicate that the the promoter trap vector PIII-myostatin could knock out the bovine myostatin gene.
Title: A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency.

we demonstrate zygote injection of TALEN mRNA can also produce gene-edited cattle and sheep. In both species we have targeted the myostatin (MSTN) gene.
Title: Genome edited sheep and cattle.

The effects of myostatin and myogenic factor 5 polymorphisms on growth and muscle traits of Marchigiana breed were assessed.
Title: Influence of single nucleotide polymorphisms in the myostatin and myogenic factor 5 muscle growth-related genes on the performance traits of Marchigiana beef cattle.

proof-of-concept study is the first to produce MSTN mutations in cattle, and may allow the development of genetically modified strains of double-muscled cattle.
Title: Efficient generation of myostatin (MSTN) biallelic mutations in cattle using zinc finger nucleases.

The following MSTN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSTN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa134047	NM_001001525.3 Latest version!	Bos taurus myostatin (MSTN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSTN ( NM_001001525.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001001525.1 Bos taurus myostatin (MSTN), mRNA.

Price & Availability↑

CloneID	OBa134047
Clone ID Related Accession (Same CDS sequence)	NM_001001525.3
Accession Version	NM_001001525.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	growth/differentiation factor 8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02003905.1 and BC134563.1. On Jun 28, 2016 this sequence version replaced NM_001001525.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC134563.1, GQ184147.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145418, SAMN03145425 [ECO:0000348] ##Evidence-Data-END##

ATGCAAAAAC TGCAAATCTC TGTTTATATT TACCTATTTA TGCTGATTGT TGCTGGCCCA 
GTGGATCTGA ATGAGAACAG CGAGCAGAAG GAAAATGTGG AAAAAGAGGG GCTGTGTAAT 
GCATGTTTGT GGAGGGAAAA CACTACATCC TCAAGACTAG AAGCCATAAA AATCCAAATC 
CTCAGTAAAC TTCGCCTGGA AACAGCTCCT AACATCAGCA AAGATGCTAT CAGACAACTT 
TTGCCCAAGG CTCCTCCACT CCTGGAACTG ATTGATCAGT TCGATGTCCA GAGAGATGCC 
AGCAGTGACG GCTCCTTGGA AGACGATGAC TACCACGCCA GGACGGAAAC GGTCATTACC 
ATGCCCACGG AGTCTGATCT TCTAACGCAA GTGGAAGGAA AACCCAAATG TTGCTTCTTT 
AAATTTAGCT CTAAGATACA ATACAATAAA CTAGTAAAGG CCCAACTGTG GATATATCTG 
AGGCCTGTCA AGACTCCTGC GACAGTGTTT GTGCAAATCC TGAGACTCAT CAAACCCATG 
AAAGACGGTA CAAGGTATAC TGGAATCCGA TCTCTGAAAC TTGACATGAA CCCAGGCACT 
GGTATTTGGC AGAGCATTGA TGTGAAGACA GTGTTGCAGA ACTGGCTCAA ACAACCTGAA 
TCCAACTTAG GCATTGAAAT CAAAGCTTTA GATGAGAATG GCCATGATCT TGCTGTAACC 
TTCCCAGAAC CAGGAGAAGA TGGACTGACT CCTTTTTTAG AAGTCAAGGT AACAGACACA 
CCAAAAAGAT CTAGGAGAGA TTTTGGGCTT GATTGTGATG AACACTCCAC AGAATCTCGA 
TGCTGTCGTT ACCCTCTAAC TGTGGATTTT GAAGCTTTTG GATGGGATTG GATTATTGCA 
CCTAAAAGAT ATAAGGCCAA TTACTGCTCT GGAGAATGTG AATTTGTATT TTTGCAAAAG 
TATCCTCATA CCCATCTTGT GCACCAAGCA AACCCCAGAG GTTCAGCCGG CCCCTGCTGT 
ACTCCTACAA AGATGTCTCC AATTAATATG CTATATTTTA ATGGCGAAGG ACAAATAATA 
TACGGGAAGA TTCCAGCCAT GGTAGTAGAT CGCTGTGGGT GTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001525.1
CDS	134..1261
Translation	MQKLQISVYIYLFMLIVAGPVDLNENSEQKENVEKEGLCNACLWRENTTSSRLEAIKIQILSKLRLETAPNISKDAIRQLLPKAPPLLELIDQFDVQRDASSDGSLEDDDYHARTETVITMPTESDLLTQVEGKPKCCFFKFSSKIQYNKLVKAQLWIYLRPVKTPATVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPEPGEDGLTPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGEGQIIYGKIPAMVVDRCGCS

Target ORF information:

RefSeq Version	NM_001001525.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myostatin (MSTN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001525.3

ORF Insert Sequence:

ATGCAAAAAC TGCAAATCTC TGTTTATATT TACCTATTTA TGCTGATTGT TGCTGGCCCA 
GTGGATCTGA ATGAGAACAG CGAGCAGAAG GAAAATGTGG AAAAAGAGGG GCTGTGTAAT 
GCATGTTTGT GGAGGGAAAA CACTACATCC TCAAGACTAG AAGCCATAAA AATCCAAATC 
CTCAGTAAAC TTCGCCTGGA AACAGCTCCT AACATCAGCA AAGATGCTAT CAGACAACTT 
TTGCCCAAGG CTCCTCCACT CCTGGAACTG ATTGATCAGT TCGATGTCCA GAGAGATGCC 
AGCAGTGACG GCTCCTTGGA AGACGATGAC TACCACGCCA GGACGGAAAC GGTCATTACC 
ATGCCCACGG AGTCTGATCT TCTAACGCAA GTGGAAGGAA AACCCAAATG TTGCTTCTTT 
AAATTTAGCT CTAAGATACA ATACAATAAA CTAGTAAAGG CCCAACTGTG GATATATCTG 
AGGCCTGTCA AGACTCCTGC GACAGTGTTT GTGCAAATCC TGAGACTCAT CAAACCCATG 
AAAGACGGTA CAAGGTATAC TGGAATCCGA TCTCTGAAAC TTGACATGAA CCCAGGCACT 
GGTATTTGGC AGAGCATTGA TGTGAAGACA GTGTTGCAGA ACTGGCTCAA ACAACCTGAA 
TCCAACTTAG GCATTGAAAT CAAAGCTTTA GATGAGAATG GCCATGATCT TGCTGTAACC 
TTCCCAGAAC CAGGAGAAGA TGGACTGACT CCTTTTTTAG AAGTCAAGGT AACAGACACA 
CCAAAAAGAT CTAGGAGAGA TTTTGGGCTT GATTGTGATG AACACTCCAC AGAATCTCGA 
TGCTGTCGTT ACCCTCTAAC TGTGGATTTT GAAGCTTTTG GATGGGATTG GATTATTGCA 
CCTAAAAGAT ATAAGGCCAA TTACTGCTCT GGAGAATGTG AATTTGTATT TTTGCAAAAG 
TATCCTCATA CCCATCTTGT GCACCAAGCA AACCCCAGAG GTTCAGCCGG CCCCTGCTGT 
ACTCCTACAA AGATGTCTCC AATTAATATG CTATATTTTA ATGGCGAAGG ACAAATAATA 
TACGGGAAGA TTCCAGCCAT GGTAGTAGAT CGCTGTGGGT GTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of Genetic Polymorphisms in GH/Hpa II and MSTN / Dra I Loci on Body Weight in Friesian Bull Calves.
Pakistan journal of biological sciences : PJBS19(8-9)338-344(2016)
Nasr SM,Ateya AI,Sadek KM,Radwan HA

A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency.
Genetics and molecular research : GMR14(1)2750-61(2015 Mar)
Zhao LH,Zhao YH,Liang H,Yun T,Han XJ,Zhang ML,Zhou X,Hou DX,Li RF,Li XL

Genome edited sheep and cattle.
Transgenic research24(1)147-53(2015 Feb)
Proudfoot C,Carlson DF,Huddart R,Long CR,Pryor JH,King TJ,Lillico SG,Mileham AJ,McLaren DG,Whitelaw CB,Fahrenkrug SC

Influence of single nucleotide polymorphisms in the myostatin and myogenic factor 5 muscle growth-related genes on the performance traits of Marchigiana beef cattle.
Journal of animal science92(9)3804-10(2014 Sep)
Sarti FM,Lasagna E,Ceccobelli S,Di Lorenzo P,Filippini F,Sbarra F,Giontella A,Pieramati C,Panella F

Efficient generation of myostatin (MSTN) biallelic mutations in cattle using zinc finger nucleases.
PloS one9(4)e95225(2014)
Luo J,Song Z,Yu S,Cui D,Wang B,Ding F,Li S,Dai Y,Li N

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MSTN cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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