Insig2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Insig2
Entrez Gene ID	288985
Full Name	insulin induced gene 2
General protein information	Preferred Names insulin induced gene 2 Names insulin-induced gene 2 protein INSIG-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q11
Summary	endoplasmic reticulum protein that blocks proteolytic activation of sterol regulatory element-binding proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249667.3	XP_006249729.1	insulin-induced gene 2 protein isoform X1
XM_017598687.1	XP_017454176.1	insulin-induced gene 2 protein isoform X1
XM_006249668.3	XP_006249730.1	insulin-induced gene 2 protein isoform X1
XM_006249669.3	XP_006249731.1	insulin-induced gene 2 protein isoform X1
NM_178091.4	NP_835192.1	insulin-induced gene 2 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	INSIG2	NP_057217.2
Rattus norvegicus (Norway rat)	Insig2	NP_835192.1
Pan troglodytes (chimpanzee)	INSIG2	XP_001155360.1
	INSIG2	XP_002799450.1
Canis lupus familiaris (dog)	INSIG2	XP_853105.1
Mus musculus (house mouse)	Insig2	NP_001258460.1
Danio rerio (zebrafish)	insig2	NP_997969.1
Bos taurus (cattle)	INSIG2	XP_003585802.1

Gene Ontology
Bibliography

Related articles in PubMed

Activation of hepatic CREBH and Insig signaling in the anti-hypertriglyceridemic mechanism of R-?-lipoic acid.
Tong X, Christian P, Zhao M, Wang H, Moreau R, Su Q
The Journal of nutritional biochemistry26(9)921-8(2015 Sep)

Evolutionary conservation and adaptation in the mechanism that regulates SREBP action: what a long, strange tRIP it's been.
Osborne TF, Espenshade PJ
Genes & development23(22)2578-91(2009 Nov)

Thermally oxidized oil increases the expression of insulin-induced genes and inhibits activation of sterol regulatory element-binding protein-2 in rat liver.
Koch A, K?nig B, Spielmann J, Leitner A, Stangl GI, Eder K
The Journal of nutrition137(9)2018-23(2007 Sep)

Severe facial clefting in Insig-deficient mouse embryos caused by sterol accumulation and reversed by lovastatin.
Engelking LJ, Evers BM, Richardson JA, Goldstein JL, Brown MS, Liang G
The Journal of clinical investigation116(9)2356-65(2006 Sep)

Docosahexaneoic acid (22:6,n-3) regulates rat hepatocyte SREBP-1 nuclear abundance by Erk- and 26S proteasome-dependent pathways.
Botolin D, Wang Y, Christian B, Jump DB
Journal of lipid research47(1)181-92(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest expression of hepatocyte Insig1/Insig2a and Crebh/Creb3l3 (cAMP responsive element-binding protein 3-like 3 protein) can be up-regulated by dietary factors (here, dietary supplementation with hypotriglyceridemic R-alpha-lipoic acid).
Title: Activation of hepatic CREBH and Insig signaling in the anti-hypertriglyceridemic mechanism of R-?-lipoic acid.

Liver-specific mRNA for Insig-2 down-regulated by insulin: implications for fatty acid synthesis.
Title: Liver-specific mRNA for Insig-2 down-regulated by insulin: implications for fatty acid synthesis.

The following Insig2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Insig2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04095	NM_178091.4 Latest version!	Rattus norvegicus insulin induced gene 2 (Insig2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa04095	XM_017598687.1 Latest version!	Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa04095	XM_006249668.3 Latest version!	Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa04095	XM_006249669.3 Latest version!	Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa04095	XM_006249667.3 Latest version!	Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Insig2 ( NM_178091.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_835192.1 Rattus norvegicus insulin induced gene 2 (Insig2), mRNA.

Price & Availability↑

CloneID	ORa04095
Clone ID Related Accession (Same CDS sequence)	XM_006249667.3 , XM_017598687.1 , XM_006249668.3 , XM_006249669.3 , NM_178091.4
Accession Version	NM_178091.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-induced gene 2 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY152392.1. On Jun 10, 2007 this sequence version replaced NM_178091.3. ##Evidence-Data-START## Transcript exon combination :: AY152392.1, CO402672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835192.1
CDS	26..703
Translation	MAEGETESPRPKKRGPYISSVTSQSVNVVIRGVVLFFIGVFLALVLNLLQIQRNVTLFPPDVITSIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNFQFSLTLAALSVGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVIAEKSHQE

Target ORF information:

RefSeq Version	NM_178091.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin induced gene 2 (Insig2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178091.4

ORF Insert Sequence:

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Insig2 ( XM_006249667.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249729.1 Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04095
Clone ID Related Accession (Same CDS sequence)	XM_006249667.3 , XM_017598687.1 , XM_006249668.3 , XM_006249669.3 , NM_178091.4
Accession Version	XM_006249667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-induced gene 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249729.1
CDS	489..1166
Translation	MAEGETESPRPKKRGPYISSVTSQSVNVVIRGVVLFFIGVFLALVLNLLQIQRNVTLFPPDVITSIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNFQFSLTLAALSVGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVIAEKSHQE

Target ORF information:

RefSeq Version	XM_006249667.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249667.3

ORF Insert Sequence:

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Insig2 ( XM_017598687.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454176.1 Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa04095
Clone ID Related Accession (Same CDS sequence)	XM_006249667.3 , XM_017598687.1 , XM_006249668.3 , XM_006249669.3 , NM_178091.4
Accession Version	XM_017598687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-induced gene 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454176.1
CDS	242..919
Translation	MAEGETESPRPKKRGPYISSVTSQSVNVVIRGVVLFFIGVFLALVLNLLQIQRNVTLFPPDVITSIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNFQFSLTLAALSVGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVIAEKSHQE

Target ORF information:

RefSeq Version	XM_017598687.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598687.1

ORF Insert Sequence:

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Insig2 ( XM_006249668.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249730.1 Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa04095
Clone ID Related Accession (Same CDS sequence)	XM_006249667.3 , XM_017598687.1 , XM_006249668.3 , XM_006249669.3 , NM_178091.4
Accession Version	XM_006249668.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-induced gene 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249730.1
CDS	327..1004
Translation	MAEGETESPRPKKRGPYISSVTSQSVNVVIRGVVLFFIGVFLALVLNLLQIQRNVTLFPPDVITSIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNFQFSLTLAALSVGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVIAEKSHQE

Target ORF information:

RefSeq Version	XM_006249668.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249668.3

ORF Insert Sequence:

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Insig2 ( XM_006249669.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249731.1 Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa04095
Clone ID Related Accession (Same CDS sequence)	XM_006249667.3 , XM_017598687.1 , XM_006249668.3 , XM_006249669.3 , NM_178091.4
Accession Version	XM_006249669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-induced gene 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249731.1
CDS	303..980
Translation	MAEGETESPRPKKRGPYISSVTSQSVNVVIRGVVLFFIGVFLALVLNLLQIQRNVTLFPPDVITSIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNFQFSLTLAALSVGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVIAEKSHQE

Target ORF information:

RefSeq Version	XM_006249669.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin induced gene 2 (Insig2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249669.3

ORF Insert Sequence:

ATGGCGGAAG GAGAGACGGA GTCACCTCGG CCTAAAAAGC GTGGCCCCTA CATTTCCTCT 
GTCACCAGCC AGAGCGTGAA CGTGGTGATC CGGGGAGTGG TGCTCTTCTT CATTGGCGTG 
TTCCTGGCTT TAGTGTTGAA CTTGCTTCAG ATTCAGAGAA ATGTGACACT GTTCCCACCA 
GATGTGATCA CCAGCATCTT CTCATCTGCA TGGTGGGTTC CACCGTGCTG CGGTACAGCC 
TCAGCTGTGA TTGGACTATT GTACCCCTGC ATTGACAGGC ATCTAGGAGA GCCACATAAA 
TTTAAGAGAG AGTGGTCCAG TGTTATGCGC TGCGTGGCCG TGTTCGTGGG TATAAATCAC 
GCCAGTGCTA AAGTCGACTT CGACAACAAC TTCCAGTTTT CCCTCACACT GGCTGCACTG 
TCAGTAGGAC TATGGTGGAC TTTTGACAGA TCTAGAAGTG GTTTTGGCCT TGGTGTTGGA 
ATTGCTTTCT TAGCAACCGT TGTCACCCAA CTGTTAGTCT ACAATGGTGT TTATCAATAC 
ACATCTCCAG ATTTTCTCTA TGTCCGCTCT TGGTTGCCAT GTATATTTTT TGCTGGAGGC 
ATAACGATGG GAAACATTGG TCGTCAGCTA GCAATGTATG AATGTAAAGT TATTGCTGAA 
AAATCTCATC AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activation of hepatic CREBH and Insig signaling in the anti-hypertriglyceridemic mechanism of R-?-lipoic acid.
The Journal of nutritional biochemistry26(9)921-8(2015 Sep)
Tong X,Christian P,Zhao M,Wang H,Moreau R,Su Q

Evolutionary conservation and adaptation in the mechanism that regulates SREBP action: what a long, strange tRIP it's been.
Genes & development23(22)2578-91(2009 Nov)
Osborne TF,Espenshade PJ

Thermally oxidized oil increases the expression of insulin-induced genes and inhibits activation of sterol regulatory element-binding protein-2 in rat liver.
The Journal of nutrition137(9)2018-23(2007 Sep)
Koch A,K?nig B,Spielmann J,Leitner A,Stangl GI,Eder K

Severe facial clefting in Insig-deficient mouse embryos caused by sterol accumulation and reversed by lovastatin.
The Journal of clinical investigation116(9)2356-65(2006 Sep)
Engelking LJ,Evers BM,Richardson JA,Goldstein JL,Brown MS,Liang G

Docosahexaneoic acid (22:6,n-3) regulates rat hepatocyte SREBP-1 nuclear abundance by Erk- and 26S proteasome-dependent pathways.
Journal of lipid research47(1)181-92(2006 Jan)
Botolin D,Wang Y,Christian B,Jump DB