Brs3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brs3
Entrez Gene ID	260319
Full Name	bombesin receptor subtype 3
General protein information	Preferred Names bombesin receptor subtype 3 Names bombesin receptor subtype-3 BRS-3 bombesin-like receptor 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq37
Summary	weakly activated by agonist dY-bombesin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_152845.1	NP_690058.1	bombesin receptor subtype-3
XM_006257677.3	XP_006257739.1	bombesin receptor subtype-3 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BRS3	XP_001137541.1
Drosophila melanogaster (fruit fly)	CCHa2r	NP_610199.2
Xenopus tropicalis (tropical clawed frog)	LOC100488712	XP_002932668.1
Canis lupus familiaris (dog)	BRS3	XP_854769.1
Mus musculus (house mouse)	Brs3	NP_033896.2
Rattus norvegicus (Norway rat)	Brs3	NP_690058.1
Gallus gallus (chicken)	BRS3	NP_989737.1
Anopheles gambiae (African malaria mosquito)	GPRGRP2	XP_313395.2
Homo sapiens (human)	BRS3	NP_001718.1
	BRS3	NP_001028074.1
Bos taurus (cattle)	BRS3	NP_001192541.1

Gene Ontology
Bibliography

Related articles in PubMed

Anatomical characterization of bombesin receptor subtype-3 mRNA expression in the rodent central nervous system.
Zhang L, Parks GS, Wang Z, Wang L, Lew M, Civelli O
The Journal of comparative neurology521(5)1020-39(2013 Apr)

Bombesin receptor subtype-3 is expressed by the enteric nervous system and by interstitial cells of Cajal in the rat gastrointestinal tract.
Porcher C, Juhem A, Peinnequin A, Bonaz B
Cell and tissue research320(1)21-31(2005 Apr)

Developmental expression and biological activity of gastrin-releasing peptide and its receptors in the kidney.
Dumesny C, Whitley JC, Baldwin GS, Giraud AS, Shulkes A
American journal of physiology. Renal physiology287(3)F578-85(2004 Sep)

The distribution of the orphan bombesin receptor subtype-3 in the rat CNS.
Jennings CA, Harrison DC, Maycox PR, Crook B, Smart D, Hervieu GJ
Neuroscience120(2)309-24(2003)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This sstudy demonistrated comprehensive mapping of BRS-3 mRNA in the rat brain and double in situ hybridization was performed to determine whether BRS-3 colocalizes with other neurotransmitters or neuropeptides.
Title: Anatomical characterization of bombesin receptor subtype-3 mRNA expression in the rodent central nervous system.

Brs3 is expressed by the enteric nervous system and by interstitial cells of Cajal in the gastrointestinal tract.
Title: Bombesin receptor subtype-3 is expressed by the enteric nervous system and by interstitial cells of Cajal in the rat gastrointestinal tract.

bombesin receptor subtype-3 (BRS-3) was detected in the rat adult and fetal kidney.
Title: Developmental expression and biological activity of gastrin-releasing peptide and its receptors in the kidney.

results indicate that the sequence variation in the E3 loop is responsible for the species difference between rat and human BRS-3, and multiple residues in the E3 loop are involved in interactions with the agonist dY-bombesin
Title: Molecular basis of the pharmacological difference between rat and human bombesin receptor subtype-3 (BRS-3).

The following Brs3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brs3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02912	NM_152845.1 Latest version!	Rattus norvegicus bombesin receptor subtype 3 (Brs3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa40299	XM_006257677.3 Latest version!	Rattus norvegicus bombesin receptor subtype 3 (Brs3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brs3 ( NM_152845.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_690058.1 Rattus norvegicus bombesin receptor subtype 3 (Brs3), mRNA.

Price & Availability↑

CloneID	ORa02912
Clone ID Related Accession (Same CDS sequence)	NM_152845.1
Accession Version	NM_152845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	bombesin receptor subtype-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF510984.1. ##Evidence-Data-START## Transcript exon combination :: AF510984.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN01906354 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCAAA GGCAGCCTCA GTCACCTAAT CAGACTTTAA TTTCCATTAC AAATGACACA 
GAAACATCAA GCTCTGCCGT CTCCAACGAT ACTACACCTA AAGGATGGAC CGGAGACAAC 
TCTCCAGGAA TAGAAGCACT GTGTGCCATC TATATCACTT ATGCTGTGAT CATTTCAGTG 
GGCATCCTCG GAAATGCTAT CCTCATCAAA GTCTTTTTCA AGACTAAATC CATGCAAACA 
GTTCCAAATA TTTTCATCAC CAGCCTGGCT TTTGGAGATC TGTTACTCCT GCTGACTTGT 
GTGCCAGTGG ATGCAACCCA CTACCTGGCA GAGGGATGGC TGTTTGGAAA GGTCGGTTGT 
AAAGTGCTTT CCTTCATCCG GCTCACTTCT GTCGGTGTAT CAGTGTTCAC GCTGACAATT 
CTCAGCGCTG ACAGATACAA AGCAGTCGTG AAGCCACTTG AACGACAGCC CTCCAATGCC 
ATTCTGAAGA CCTGTGCCAA AGCTGGTGGC ATCTGGATCA TGGCTATGAT ATTTGCTCTG 
CCAGAGGCTA TATTCTCAAA TGTATACACT TTCCAAGATC CTAACAGAAA CGTAACATTT 
GAATCCTGTA ACTCCTACCC TATCTCTGAG AGGCTTTTGC AGGAAATACA TTCTCTGTTG 
TGTTTCTTGG TGTTCTACAT TATCCCGCTC TCGATTATCT CTGTCTATTA TTCTTTGATT 
GCCAGGACTC TTTACAAAAG CACCTTGAAC ATACCGACTG AGGAACAAAG CCATGCCCGA 
AAGCAGATTG AATCCCGGAA GAGAATTGCC AAAACGGTAC TGGTGCTGGT GGCTCTGTTC 
GCACTCTGCT GGTTGCCGAA TCACCTCCTG TATCTCTATC ACTCATTCAC TTATGAAAGC 
TACGCAGAGC CTTCTGATGT CCCTTTCGTT GTCACCATTT TCTCTCGGGT GCTGGCTTTC 
AGTAATTCCT GCGTGAACCC CTTTGCTCTG TATTGGCTGA GCAAGACCTT CCAGAAGCAT 
TTTAAGGCTC AGCTCTGCTG CTTCAAGGCA GAGCAGCCTG AACCTCCTCT TGGTGACACC 
CCCCTTAACA ACCTCACTGT GATGGGGCGG GTTCCAGCTA CTGGGAGTGC ACACGTCTCT 
GAAATTAGCG TGACCCTGTT TAGTGGCAGT ACTGCCAAGA AAGGAGAGGA CAAAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690058.1
CDS	121..1320
Translation	MSQRQPQSPNQTLISITNDTETSSSAVSNDTTPKGWTGDNSPGIEALCAIYITYAVIISVGILGNAILIKVFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGKVGCKVLSFIRLTSVGVSVFTLTILSADRYKAVVKPLERQPSNAILKTCAKAGGIWIMAMIFALPEAIFSNVYTFQDPNRNVTFESCNSYPISERLLQEIHSLLCFLVFYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIAKTVLVLVALFALCWLPNHLLYLYHSFTYESYAEPSDVPFVVTIFSRVLAFSNSCVNPFALYWLSKTFQKHFKAQLCCFKAEQPEPPLGDTPLNNLTVMGRVPATGSAHVSEISVTLFSGSTAKKGEDKV

Target ORF information:

RefSeq Version	NM_152845.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bombesin receptor subtype 3 (Brs3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152845.1

ORF Insert Sequence:

ATGTCTCAAA GGCAGCCTCA GTCACCTAAT CAGACTTTAA TTTCCATTAC AAATGACACA 
GAAACATCAA GCTCTGCCGT CTCCAACGAT ACTACACCTA AAGGATGGAC CGGAGACAAC 
TCTCCAGGAA TAGAAGCACT GTGTGCCATC TATATCACTT ATGCTGTGAT CATTTCAGTG 
GGCATCCTCG GAAATGCTAT CCTCATCAAA GTCTTTTTCA AGACTAAATC CATGCAAACA 
GTTCCAAATA TTTTCATCAC CAGCCTGGCT TTTGGAGATC TGTTACTCCT GCTGACTTGT 
GTGCCAGTGG ATGCAACCCA CTACCTGGCA GAGGGATGGC TGTTTGGAAA GGTCGGTTGT 
AAAGTGCTTT CCTTCATCCG GCTCACTTCT GTCGGTGTAT CAGTGTTCAC GCTGACAATT 
CTCAGCGCTG ACAGATACAA AGCAGTCGTG AAGCCACTTG AACGACAGCC CTCCAATGCC 
ATTCTGAAGA CCTGTGCCAA AGCTGGTGGC ATCTGGATCA TGGCTATGAT ATTTGCTCTG 
CCAGAGGCTA TATTCTCAAA TGTATACACT TTCCAAGATC CTAACAGAAA CGTAACATTT 
GAATCCTGTA ACTCCTACCC TATCTCTGAG AGGCTTTTGC AGGAAATACA TTCTCTGTTG 
TGTTTCTTGG TGTTCTACAT TATCCCGCTC TCGATTATCT CTGTCTATTA TTCTTTGATT 
GCCAGGACTC TTTACAAAAG CACCTTGAAC ATACCGACTG AGGAACAAAG CCATGCCCGA 
AAGCAGATTG AATCCCGGAA GAGAATTGCC AAAACGGTAC TGGTGCTGGT GGCTCTGTTC 
GCACTCTGCT GGTTGCCGAA TCACCTCCTG TATCTCTATC ACTCATTCAC TTATGAAAGC 
TACGCAGAGC CTTCTGATGT CCCTTTCGTT GTCACCATTT TCTCTCGGGT GCTGGCTTTC 
AGTAATTCCT GCGTGAACCC CTTTGCTCTG TATTGGCTGA GCAAGACCTT CCAGAAGCAT 
TTTAAGGCTC AGCTCTGCTG CTTCAAGGCA GAGCAGCCTG AACCTCCTCT TGGTGACACC 
CCCCTTAACA ACCTCACTGT GATGGGGCGG GTTCCAGCTA CTGGGAGTGC ACACGTCTCT 
GAAATTAGCG TGACCCTGTT TAGTGGCAGT ACTGCCAAGA AAGGAGAGGA CAAAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brs3 ( XM_006257677.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257739.1 Rattus norvegicus bombesin receptor subtype 3 (Brs3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa40299
Clone ID Related Accession (Same CDS sequence)	XM_006257677.3
Accession Version	XM_006257677.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bombesin receptor subtype-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257677.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTG AAACATCAAG CTCTGCCGTC TCCAACGATA CTACACCTAA AGGATGGACC 
GGAGACAACT CTCCAGGAAT AGAAGCACTG TGTGCCATCT ATATCACTTA TGCTGTGATC 
ATTTCAGTGG GCATCCTCGG AAATGCTATC CTCATCAAAG TCTTTTTCAA GACTAAATCC 
ATGCAAACAG TTCCAAATAT TTTCATCACC AGCCTGGCTT TTGGAGATCT GTTACTCCTG 
CTGACTTGTG TGCCAGTGGA TGCAACCCAC TACCTGGCAG AGGGATGGCT GTTTGGAAAG 
GTCGGTTGTA AAGTGCTTTC CTTCATCCGG CTCACTTCTG TCGGTGTATC AGTGTTCACG 
CTAACAATTC TCAGCGCTGA CAGATACAAA GCAGTCGTGA AGCCACTTGA ACGACAGCCC 
TCCAATGCCA TTCTGAAGAC CTGTGCCAAA GCTGGTGGCA TCTGGATCAT GGCTATGATA 
TTTGCTCTGC CAGAGGCTAT ATTCTCAAAT GTATACACTT TCCAAGATCC TAACAGAAAC 
GTAACATTTG AATCCTGTAA CTCCTACCCT ATCTCTGAGA GGCTTTTGCA GGAAATACAT 
TCTCTGTTGT GTTTCTTGGT GTTCTACATT ATCCCGCTCT CGATTATCTC TGTCTATTAT 
TCTTTGATTG CCAGGACTCT TTACAAAAGC ACCTTGAACA TACCGACTGA GGAACAAAGC 
CATGCCCGAA AGCAGATTGA ATCCCGGAAG AGAATTGCCA AAACGGTACT GGTGCTGGTG 
GCTCTGTTCG CACTCTGCTG GTTGCCGAAT CACCTCCTGT ATCTCTATCA CTCATTCACT 
TATGAAAGCT ACGCAGAGCC TTCTGATGTC CCTTTCGTTG TCACCATTTT CTCTCGGGTG 
CTGGCTTTCA GTAATTCCTG CGTGAACCCC TTTGCTCTGT ATTGGCTGAG CAAGACCTTC 
CAGAAGCATT TTAAGGCTCA GCTCTGCTGC TTCAAGGCAG AGCAGCCTGA GCCTCCTCTT 
GGTGACACCC CCCTTAACAA CCTCACTGTG ATGGGGCGGG TTCCAGCTAC TGGGAGTGCA 
CACGTCTCTG AAATTAGCGT GACCCTGTTT AGTGGCAGTA CTGCCAAGAA AGGAGAGGAC 
AAAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257739.1
CDS	203..1351
Translation	MATETSSSAVSNDTTPKGWTGDNSPGIEALCAIYITYAVIISVGILGNAILIKVFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGKVGCKVLSFIRLTSVGVSVFTLTILSADRYKAVVKPLERQPSNAILKTCAKAGGIWIMAMIFALPEAIFSNVYTFQDPNRNVTFESCNSYPISERLLQEIHSLLCFLVFYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIAKTVLVLVALFALCWLPNHLLYLYHSFTYESYAEPSDVPFVVTIFSRVLAFSNSCVNPFALYWLSKTFQKHFKAQLCCFKAEQPEPPLGDTPLNNLTVMGRVPATGSAHVSEISVTLFSGSTAKKGEDKV

Target ORF information:

RefSeq Version	XM_006257677.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bombesin receptor subtype 3 (Brs3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257677.3

ORF Insert Sequence:

ATGGCAACTG AAACATCAAG CTCTGCCGTC TCCAACGATA CTACACCTAA AGGATGGACC 
GGAGACAACT CTCCAGGAAT AGAAGCACTG TGTGCCATCT ATATCACTTA TGCTGTGATC 
ATTTCAGTGG GCATCCTCGG AAATGCTATC CTCATCAAAG TCTTTTTCAA GACTAAATCC 
ATGCAAACAG TTCCAAATAT TTTCATCACC AGCCTGGCTT TTGGAGATCT GTTACTCCTG 
CTGACTTGTG TGCCAGTGGA TGCAACCCAC TACCTGGCAG AGGGATGGCT GTTTGGAAAG 
GTCGGTTGTA AAGTGCTTTC CTTCATCCGG CTCACTTCTG TCGGTGTATC AGTGTTCACG 
CTAACAATTC TCAGCGCTGA CAGATACAAA GCAGTCGTGA AGCCACTTGA ACGACAGCCC 
TCCAATGCCA TTCTGAAGAC CTGTGCCAAA GCTGGTGGCA TCTGGATCAT GGCTATGATA 
TTTGCTCTGC CAGAGGCTAT ATTCTCAAAT GTATACACTT TCCAAGATCC TAACAGAAAC 
GTAACATTTG AATCCTGTAA CTCCTACCCT ATCTCTGAGA GGCTTTTGCA GGAAATACAT 
TCTCTGTTGT GTTTCTTGGT GTTCTACATT ATCCCGCTCT CGATTATCTC TGTCTATTAT 
TCTTTGATTG CCAGGACTCT TTACAAAAGC ACCTTGAACA TACCGACTGA GGAACAAAGC 
CATGCCCGAA AGCAGATTGA ATCCCGGAAG AGAATTGCCA AAACGGTACT GGTGCTGGTG 
GCTCTGTTCG CACTCTGCTG GTTGCCGAAT CACCTCCTGT ATCTCTATCA CTCATTCACT 
TATGAAAGCT ACGCAGAGCC TTCTGATGTC CCTTTCGTTG TCACCATTTT CTCTCGGGTG 
CTGGCTTTCA GTAATTCCTG CGTGAACCCC TTTGCTCTGT ATTGGCTGAG CAAGACCTTC 
CAGAAGCATT TTAAGGCTCA GCTCTGCTGC TTCAAGGCAG AGCAGCCTGA GCCTCCTCTT 
GGTGACACCC CCCTTAACAA CCTCACTGTG ATGGGGCGGG TTCCAGCTAC TGGGAGTGCA 
CACGTCTCTG AAATTAGCGT GACCCTGTTT AGTGGCAGTA CTGCCAAGAA AGGAGAGGAC 
AAAGTTTAG