Eef2k cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eef2k
Entrez Gene ID	25435
Full Name	eukaryotic elongation factor-2 kinase
Synonyms	SMEF2K
General protein information	Preferred Names eukaryotic elongation factor-2 kinase Names eukaryotic elongation factor 2 kinase calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase eEF-2 kinase eEF-2K elongation factor 2 kinase eukaroytic elongation factor 2 kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q36
Summary	catalyzes the phosphorylation of eukaryotic elongation factor 2 (eEF2) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006230156.3	XP_006230218.1	eukaryotic elongation factor 2 kinase isoform X1
XM_006230158.3	XP_006230220.1	eukaryotic elongation factor 2 kinase isoform X1
XM_006230157.3	XP_006230219.1	eukaryotic elongation factor 2 kinase isoform X1
NM_012947.2	NP_037079.1	eukaryotic elongation factor 2 kinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	EEF2K	NP_037434.1
Bos taurus (cattle)	EEF2K	NP_001179471.1
	EEF2K	XP_001092565.1
Canis lupus familiaris (dog)	EEF2K	XP_005621491.1
Pan troglodytes (chimpanzee)	EEF2K	XP_001161085.2
Rattus norvegicus (Norway rat)	Eef2k	NP_037079.1
Danio rerio (zebrafish)	eef2k	NP_001002740.1
Caenorhabditis elegans (roundworm)	efk-1	NP_001022572.1
Xenopus tropicalis (tropical clawed frog)	eef2k	NP_001120146.1
Mus musculus (house mouse)	Eef2k	NP_001254640.1
Gallus gallus (chicken)	EEF2K	XP_004945358.1

Gene Ontology
Bibliography

Related articles in PubMed

Heat shock protein 70 protects cardiomyocytes through suppressing SUMOylation and nucleus translocation of phosphorylated eukaryotic elongation factor 2 during myocardial ischemia and reperfusion.
Zhang C, Liu X, Miao J, Wang S, Wu L, Yan D, Li J, Guo W, Wu X, Shen A
Apoptosis : an international journal on programmed cell death22(5)608-625(2017 May)

eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.
Heise C, Taha E, Murru L, Ponzoni L, Cattaneo A, Guarnieri FC, Montani C, Mossa A, Vezzoli E, Ippolito G, Zapata J, Barrera I, Ryazanov AG, Cook J, Poe M, Stephen MR, Kopanitsa M, Benfante R, Rusconi F, Braida D, Francolini M, Proud CG, Valtorta F, Passafaro M, Sala M, Bachi A, Verpelli C, Rosenblum K, Sala C
Cerebral cortex (New York, N.Y. : 1991)27(3)2226-2248(2017 Mar)

Intrauterine growth restriction inhibits expression of eukaryotic elongation factor 2 kinase, a regulator of protein translation.
McKnight RA, Yost CC, Zinkhan EK, Fu Q, Callaway CW, Fung CM
Physiological genomics48(8)616-25(2016 Aug)

Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes.
Andreev DE, O'Connor PB, Zhdanov AV, Dmitriev RI, Shatsky IN, Papkovsky DB, Baranov PV
Genome biology1690(2015 May)

Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation.
Usui T, Okada M, Hara Y, Yamawaki H
American journal of physiology. Heart and circulatory physiology305(5)H756-68(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using an eEF2K gain-of-function design in vitro as well as eEF2K-KO mice, we show that eEF2K activity regulates the excitation/inhibition balance by downregulating vesicle release at inhibitory synapses and tonic inhibition. This translates into a reduced susceptibility of eEF2K-KO mice for seizures without major behavioral alterations with the exception of impaired contextual and trace fear memory
Title: eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.

Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation.
Title: Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation.

Expression of betaTRCP1 increases the polyubiquitylation of EEF2K in PC12 cells.
Title: Proteasomal degradation of eukaryotic elongation factor-2 kinase (EF2K) is regulated by cAMP-PKA signaling and the SCF?TRCP ubiquitin E3 ligase.

A Ca(2+)-calmodulin-eEF2K-eEF2 signalling cascade, but not AMPK, contributes to the suppression of skeletal muscle protein synthesis during contractions.
Title: A Ca(2+)-calmodulin-eEF2K-eEF2 signalling cascade, but not AMPK, contributes to the suppression of skeletal muscle protein synthesis during contractions.

AMPK and eEF2 kinase may provide a key link between cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy
Title: Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398.

The following Eef2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eef2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01412	NM_012947.2 Latest version!	Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01412	XM_006230157.3 Latest version!	Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01412	XM_006230156.3 Latest version!	Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01412	XM_006230158.3 Latest version!	Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eef2k ( NM_012947.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037079.1 Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), mRNA.

Price & Availability↑

CloneID	ORa01412
Clone ID Related Accession (Same CDS sequence)	XM_006230156.3 , XM_006230158.3 , XM_006230157.3 , NM_012947.2
Accession Version	NM_012947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic elongation factor 2 kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U93849.1. On Jun 10, 2007 this sequence version replaced NM_012947.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U93849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGACG AAGACCTCAT CTTCCGCCTG GAAGGTGTTG ACGGTGGCGG GTCCTCCGGA 
GCTGGCCGCC ATGGGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA CTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAACCAG AATGTCAACT CCAAAGGCCA GGGCTACTAC 
AACAACCTAC TGAAAACGGA GTGTGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAGGCCT GGAAGCATGC GATCGAGAAG GCCAAGCACA TGCCCGACCC CTGGGCTGAA 
TTCCATCTGG AGGACATCGC CACAGAGCAT GCTACTCGGC ACAGGTACAA TGCTGTCACC 
GGGGAGTGGC TGAAAGACGA GGTTCTGATC AAGATGGCAT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCATGC CCAGCATTGG 
AAGGGGGCCT CCAACTACGT GGCAAAACGC TACCTCGAGC CAGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAAGCG AAGCTCTGGG GGGAGGAGTA CAACCGGCAC 
AAGCCCCCCA AGCAGGTAGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACAA 
GGACAGCCCC TCTTCCACCT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTATAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC AGACTAACAC CACAGGCCTT CAGCCATTTC 
ACATTTGAAC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGCGACCTT 
TATACTGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGTATTTG CCAGAGCATG 
GGCCTTGCGC CCTTTGACCT CTCCCCACGG GAGCAGGATG CCGTGAACCA GAGCACCAAG 
CTGTTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTGGCAG CCGGCCCCCC TTGCTCCTTC GCCTGTCAGA GAACTCTGGG 
GATGAGAACA TGAGTGATGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCCACA 
CCACACAGCC AGAAGTTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAATGGCA ACCGAAGGCC TGAATCTGAT GAGGACAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCCAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAAAG GTTAAATGCT CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATCT GGCCATGGTA CGGTACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AATGGGATCA AGAGTCTGCC ATCTTCCATC TGGAGCATGC AGCCGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGGA GGAGACAGAA GAGAACAAGA CAAAAGGCTT TGATTACCTC 
CTGAAGGCCG CAGAAGCTGG TGACAGGCAG TCCATGATTT TAGTGGCTCG AGCTTTTGAC 
ACCGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT ACACTGGTAC 
AACACGGCCC TGGAGACGAC GGACTGCGAT GAAGGCGGGG AGTATGACGG AATACAGGAC 
GAGCCCCAGT ATGCACTGCT GGCCAGGGAG GCAGAGATGT TGCTCACCGG TGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAC TTGTACACCC AGGCAGCCGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTATGAGA AGGCGGAAGA GGCCTGGGCG 
CAAATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037079.1
CDS	1..2175
Translation	MADEDLIFRLEGVDGGGSSGAGRHGDSDTDSDDDEGYFICPITDDHMSNQNVNSKGQGYYNNLLKTECGSTGSPASSFHFKEAWKHAIEKAKHMPDPWAEFHLEDIATEHATRHRYNAVTGEWLKDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQHWKGASNYVAKRYLEPVDRSVYFEDVQLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRQGQPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTEKGTDFGDGNLGVRGMALFFYSHACNRICQSMGLAPFDLSPREQDAVNQSTKLLQSAKTILRGTEEKCGSPRIRTLSGSRPPLLLRLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFGDLDNMGPRDHDRMDNHRDSENSGDSGYPSEKRSDLDDPEPREHGHSNGNRRPESDEDSLGSSGRVCVETWNLLNPSRLHLPRPSAVALEVQRLNALDLGRKIGKSVLGKVHLAMVRYHEGGRFCEKDEEWDQESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLEETEENKTKGFDYLLKAAEAGDRQSMILVARAFDTGLNLSPDRCQDWSEALHWYNTALETTDCDEGGEYDGIQDEPQYALLAREAEMLLTGGFGLDKNPQRSGDLYTQAAEAAMEAMKGRLANQYYEKAEEAWAQMEE

Target ORF information:

RefSeq Version	NM_012947.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012947.2

ORF Insert Sequence:

ATGGCAGACG AAGACCTCAT CTTCCGCCTG GAAGGTGTTG ACGGTGGCGG GTCCTCCGGA 
GCTGGCCGCC ATGGGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA CTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAACCAG AATGTCAACT CCAAAGGCCA GGGCTACTAC 
AACAACCTAC TGAAAACGGA GTGTGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAGGCCT GGAAGCATGC GATCGAGAAG GCCAAGCACA TGCCCGACCC CTGGGCTGAA 
TTCCATCTGG AGGACATCGC CACAGAGCAT GCTACTCGGC ACAGGTACAA TGCTGTCACC 
GGGGAGTGGC TGAAAGACGA GGTTCTGATC AAGATGGCAT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCATGC CCAGCATTGG 
AAGGGGGCCT CCAACTACGT GGCAAAACGC TACCTCGAGC CAGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAAGCG AAGCTCTGGG GGGAGGAGTA CAACCGGCAC 
AAGCCCCCCA AGCAGGTAGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACAA 
GGACAGCCCC TCTTCCACCT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTATAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC AGACTAACAC CACAGGCCTT CAGCCATTTC 
ACATTTGAAC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGCGACCTT 
TATACTGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGTATTTG CCAGAGCATG 
GGCCTTGCGC CCTTTGACCT CTCCCCACGG GAGCAGGATG CCGTGAACCA GAGCACCAAG 
CTGTTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTGGCAG CCGGCCCCCC TTGCTCCTTC GCCTGTCAGA GAACTCTGGG 
GATGAGAACA TGAGTGATGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCCACA 
CCACACAGCC AGAAGTTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAATGGCA ACCGAAGGCC TGAATCTGAT GAGGACAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCCAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAAAG GTTAAATGCT CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATCT GGCCATGGTA CGGTACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AATGGGATCA AGAGTCTGCC ATCTTCCATC TGGAGCATGC AGCCGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGGA GGAGACAGAA GAGAACAAGA CAAAAGGCTT TGATTACCTC 
CTGAAGGCCG CAGAAGCTGG TGACAGGCAG TCCATGATTT TAGTGGCTCG AGCTTTTGAC 
ACCGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT ACACTGGTAC 
AACACGGCCC TGGAGACGAC GGACTGCGAT GAAGGCGGGG AGTATGACGG AATACAGGAC 
GAGCCCCAGT ATGCACTGCT GGCCAGGGAG GCAGAGATGT TGCTCACCGG TGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAC TTGTACACCC AGGCAGCCGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTATGAGA AGGCGGAAGA GGCCTGGGCG 
CAAATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eef2k ( XM_006230156.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230218.1 Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01412
Clone ID Related Accession (Same CDS sequence)	XM_006230156.3 , XM_006230158.3 , XM_006230157.3 , NM_012947.2
Accession Version	XM_006230156.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic elongation factor 2 kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230156.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG AAGACCTCAT CTTCCGCCTG GAAGGTGTTG ACGGTGGCGG GTCCTCCGGA 
GCTGGCCGCC ATGGGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA CTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAACCAG AATGTCAACT CCAAAGGCCA GGGCTACTAC 
AACAACCTAC TGAAAACGGA GTGTGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAGGCCT GGAAGCATGC GATCGAGAAG GCCAAGCACA TGCCCGACCC CTGGGCTGAA 
TTCCATCTGG AGGACATCGC CACAGAGCAT GCTACTCGGC ACAGGTACAA TGCTGTCACC 
GGGGAGTGGC TGAAAGACGA GGTTCTGATC AAGATGGCAT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCATGC CCAGCATTGG 
AAGGGGGCCT CCAACTACGT GGCAAAACGC TACCTCGAGC CAGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAAGCG AAGCTCTGGG GGGAGGAGTA CAACCGGCAC 
AAGCCCCCCA AGCAGGTAGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACAA 
GGACAGCCCC TCTTCCACCT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTATAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC AGACTAACAC CACAGGCCTT CAGCCATTTC 
ACATTTGAAC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGCGACCTT 
TATACTGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGTATTTG CCAGAGCATG 
GGCCTTGCGC CCTTTGACCT CTCCCCACGG GAGCAGGATG CCGTGAACCA GAGCACCAAG 
CTGTTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTGGCAG CCGGCCCCCC TTGCTCCTTC GCCTGTCAGA GAACTCTGGG 
GATGAGAACA TGAGTGATGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCCACA 
CCACACAGCC AGAAGTTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAATGGCA ACCGAAGGCC TGAATCTGAT GAGGACAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCCAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAAAG GTTAAATGCT CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATCT GGCCATGGTA CGGTACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AATGGGATCA AGAGTCTGCC ATCTTCCATC TGGAGCATGC AGCCGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGGA GGAGACAGAA GAGAACAAGA CAAAAGGCTT TGATTACCTC 
CTGAAGGCCG CAGAAGCTGG TGACAGGCAG TCCATGATTT TAGTGGCTCG AGCTTTTGAC 
ACCGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT ACACTGGTAC 
AACACGGCCC TGGAGACGAC GGACTGCGAT GAAGGCGGGG AGTATGACGG AATACAGGAC 
GAGCCCCAGT ATGCACTGCT GGCCAGGGAG GCAGAGATGT TGCTCACCGG TGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAC TTGTACACCC AGGCAGCCGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTATGAGA AGGCGGAAGA GGCCTGGGCG 
CAAATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230218.1
CDS	325..2499
Translation	MADEDLIFRLEGVDGGGSSGAGRHGDSDTDSDDDEGYFICPITDDHMSNQNVNSKGQGYYNNLLKTECGSTGSPASSFHFKEAWKHAIEKAKHMPDPWAEFHLEDIATEHATRHRYNAVTGEWLKDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQHWKGASNYVAKRYLEPVDRSVYFEDVQLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRQGQPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTEKGTDFGDGNLGVRGMALFFYSHACNRICQSMGLAPFDLSPREQDAVNQSTKLLQSAKTILRGTEEKCGSPRIRTLSGSRPPLLLRLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFGDLDNMGPRDHDRMDNHRDSENSGDSGYPSEKRSDLDDPEPREHGHSNGNRRPESDEDSLGSSGRVCVETWNLLNPSRLHLPRPSAVALEVQRLNALDLGRKIGKSVLGKVHLAMVRYHEGGRFCEKDEEWDQESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLEETEENKTKGFDYLLKAAEAGDRQSMILVARAFDTGLNLSPDRCQDWSEALHWYNTALETTDCDEGGEYDGIQDEPQYALLAREAEMLLTGGFGLDKNPQRSGDLYTQAAEAAMEAMKGRLANQYYEKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_006230156.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230156.3

ORF Insert Sequence:

ATGGCAGACG AAGACCTCAT CTTCCGCCTG GAAGGTGTTG ACGGTGGCGG GTCCTCCGGA 
GCTGGCCGCC ATGGGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA CTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAACCAG AATGTCAACT CCAAAGGCCA GGGCTACTAC 
AACAACCTAC TGAAAACGGA GTGTGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAGGCCT GGAAGCATGC GATCGAGAAG GCCAAGCACA TGCCCGACCC CTGGGCTGAA 
TTCCATCTGG AGGACATCGC CACAGAGCAT GCTACTCGGC ACAGGTACAA TGCTGTCACC 
GGGGAGTGGC TGAAAGACGA GGTTCTGATC AAGATGGCAT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCATGC CCAGCATTGG 
AAGGGGGCCT CCAACTACGT GGCAAAACGC TACCTCGAGC CAGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAAGCG AAGCTCTGGG GGGAGGAGTA CAACCGGCAC 
AAGCCCCCCA AGCAGGTAGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACAA 
GGACAGCCCC TCTTCCACCT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTATAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC AGACTAACAC CACAGGCCTT CAGCCATTTC 
ACATTTGAAC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGCGACCTT 
TATACTGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGTATTTG CCAGAGCATG 
GGCCTTGCGC CCTTTGACCT CTCCCCACGG GAGCAGGATG CCGTGAACCA GAGCACCAAG 
CTGTTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTGGCAG CCGGCCCCCC TTGCTCCTTC GCCTGTCAGA GAACTCTGGG 
GATGAGAACA TGAGTGATGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCCACA 
CCACACAGCC AGAAGTTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAATGGCA ACCGAAGGCC TGAATCTGAT GAGGACAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCCAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAAAG GTTAAATGCT CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATCT GGCCATGGTA CGGTACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AATGGGATCA AGAGTCTGCC ATCTTCCATC TGGAGCATGC AGCCGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGGA GGAGACAGAA GAGAACAAGA CAAAAGGCTT TGATTACCTC 
CTGAAGGCCG CAGAAGCTGG TGACAGGCAG TCCATGATTT TAGTGGCTCG AGCTTTTGAC 
ACCGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT ACACTGGTAC 
AACACGGCCC TGGAGACGAC GGACTGCGAT GAAGGCGGGG AGTATGACGG AATACAGGAC 
GAGCCCCAGT ATGCACTGCT GGCCAGGGAG GCAGAGATGT TGCTCACCGG TGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAC TTGTACACCC AGGCAGCCGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTATGAGA AGGCGGAAGA GGCCTGGGCG 
CAAATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eef2k ( XM_006230158.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230220.1 Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa01412
Clone ID Related Accession (Same CDS sequence)	XM_006230156.3 , XM_006230158.3 , XM_006230157.3 , NM_012947.2
Accession Version	XM_006230158.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic elongation factor 2 kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230158.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG AAGACCTCAT CTTCCGCCTG GAAGGTGTTG ACGGTGGCGG GTCCTCCGGA 
GCTGGCCGCC ATGGGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA CTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAACCAG AATGTCAACT CCAAAGGCCA GGGCTACTAC 
AACAACCTAC TGAAAACGGA GTGTGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAGGCCT GGAAGCATGC GATCGAGAAG GCCAAGCACA TGCCCGACCC CTGGGCTGAA 
TTCCATCTGG AGGACATCGC CACAGAGCAT GCTACTCGGC ACAGGTACAA TGCTGTCACC 
GGGGAGTGGC TGAAAGACGA GGTTCTGATC AAGATGGCAT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCATGC CCAGCATTGG 
AAGGGGGCCT CCAACTACGT GGCAAAACGC TACCTCGAGC CAGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAAGCG AAGCTCTGGG GGGAGGAGTA CAACCGGCAC 
AAGCCCCCCA AGCAGGTAGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACAA 
GGACAGCCCC TCTTCCACCT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTATAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC AGACTAACAC CACAGGCCTT CAGCCATTTC 
ACATTTGAAC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGCGACCTT 
TATACTGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGTATTTG CCAGAGCATG 
GGCCTTGCGC CCTTTGACCT CTCCCCACGG GAGCAGGATG CCGTGAACCA GAGCACCAAG 
CTGTTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTGGCAG CCGGCCCCCC TTGCTCCTTC GCCTGTCAGA GAACTCTGGG 
GATGAGAACA TGAGTGATGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCCACA 
CCACACAGCC AGAAGTTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAATGGCA ACCGAAGGCC TGAATCTGAT GAGGACAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCCAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAAAG GTTAAATGCT CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATCT GGCCATGGTA CGGTACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AATGGGATCA AGAGTCTGCC ATCTTCCATC TGGAGCATGC AGCCGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGGA GGAGACAGAA GAGAACAAGA CAAAAGGCTT TGATTACCTC 
CTGAAGGCCG CAGAAGCTGG TGACAGGCAG TCCATGATTT TAGTGGCTCG AGCTTTTGAC 
ACCGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT ACACTGGTAC 
AACACGGCCC TGGAGACGAC GGACTGCGAT GAAGGCGGGG AGTATGACGG AATACAGGAC 
GAGCCCCAGT ATGCACTGCT GGCCAGGGAG GCAGAGATGT TGCTCACCGG TGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAC TTGTACACCC AGGCAGCCGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTATGAGA AGGCGGAAGA GGCCTGGGCG 
CAAATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230220.1
CDS	1397..3571
Translation	MADEDLIFRLEGVDGGGSSGAGRHGDSDTDSDDDEGYFICPITDDHMSNQNVNSKGQGYYNNLLKTECGSTGSPASSFHFKEAWKHAIEKAKHMPDPWAEFHLEDIATEHATRHRYNAVTGEWLKDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQHWKGASNYVAKRYLEPVDRSVYFEDVQLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRQGQPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTEKGTDFGDGNLGVRGMALFFYSHACNRICQSMGLAPFDLSPREQDAVNQSTKLLQSAKTILRGTEEKCGSPRIRTLSGSRPPLLLRLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFGDLDNMGPRDHDRMDNHRDSENSGDSGYPSEKRSDLDDPEPREHGHSNGNRRPESDEDSLGSSGRVCVETWNLLNPSRLHLPRPSAVALEVQRLNALDLGRKIGKSVLGKVHLAMVRYHEGGRFCEKDEEWDQESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLEETEENKTKGFDYLLKAAEAGDRQSMILVARAFDTGLNLSPDRCQDWSEALHWYNTALETTDCDEGGEYDGIQDEPQYALLAREAEMLLTGGFGLDKNPQRSGDLYTQAAEAAMEAMKGRLANQYYEKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_006230158.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230158.3

ORF Insert Sequence:

ATGGCAGACG AAGACCTCAT CTTCCGCCTG GAAGGTGTTG ACGGTGGCGG GTCCTCCGGA 
GCTGGCCGCC ATGGGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA CTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAACCAG AATGTCAACT CCAAAGGCCA GGGCTACTAC 
AACAACCTAC TGAAAACGGA GTGTGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAGGCCT GGAAGCATGC GATCGAGAAG GCCAAGCACA TGCCCGACCC CTGGGCTGAA 
TTCCATCTGG AGGACATCGC CACAGAGCAT GCTACTCGGC ACAGGTACAA TGCTGTCACC 
GGGGAGTGGC TGAAAGACGA GGTTCTGATC AAGATGGCAT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCATGC CCAGCATTGG 
AAGGGGGCCT CCAACTACGT GGCAAAACGC TACCTCGAGC CAGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAAGCG AAGCTCTGGG GGGAGGAGTA CAACCGGCAC 
AAGCCCCCCA AGCAGGTAGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACAA 
GGACAGCCCC TCTTCCACCT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTATAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC AGACTAACAC CACAGGCCTT CAGCCATTTC 
ACATTTGAAC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGCGACCTT 
TATACTGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGTATTTG CCAGAGCATG 
GGCCTTGCGC CCTTTGACCT CTCCCCACGG GAGCAGGATG CCGTGAACCA GAGCACCAAG 
CTGTTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTGGCAG CCGGCCCCCC TTGCTCCTTC GCCTGTCAGA GAACTCTGGG 
GATGAGAACA TGAGTGATGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCCACA 
CCACACAGCC AGAAGTTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAATGGCA ACCGAAGGCC TGAATCTGAT GAGGACAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCCAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAAAG GTTAAATGCT CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATCT GGCCATGGTA CGGTACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AATGGGATCA AGAGTCTGCC ATCTTCCATC TGGAGCATGC AGCCGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGGA GGAGACAGAA GAGAACAAGA CAAAAGGCTT TGATTACCTC 
CTGAAGGCCG CAGAAGCTGG TGACAGGCAG TCCATGATTT TAGTGGCTCG AGCTTTTGAC 
ACCGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT ACACTGGTAC 
AACACGGCCC TGGAGACGAC GGACTGCGAT GAAGGCGGGG AGTATGACGG AATACAGGAC 
GAGCCCCAGT ATGCACTGCT GGCCAGGGAG GCAGAGATGT TGCTCACCGG TGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAC TTGTACACCC AGGCAGCCGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTATGAGA AGGCGGAAGA GGCCTGGGCG 
CAAATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eef2k ( XM_006230157.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230219.1 Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa01412
Clone ID Related Accession (Same CDS sequence)	XM_006230156.3 , XM_006230158.3 , XM_006230157.3 , NM_012947.2
Accession Version	XM_006230157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic elongation factor 2 kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230157.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG AAGACCTCAT CTTCCGCCTG GAAGGTGTTG ACGGTGGCGG GTCCTCCGGA 
GCTGGCCGCC ATGGGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA CTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAACCAG AATGTCAACT CCAAAGGCCA GGGCTACTAC 
AACAACCTAC TGAAAACGGA GTGTGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAGGCCT GGAAGCATGC GATCGAGAAG GCCAAGCACA TGCCCGACCC CTGGGCTGAA 
TTCCATCTGG AGGACATCGC CACAGAGCAT GCTACTCGGC ACAGGTACAA TGCTGTCACC 
GGGGAGTGGC TGAAAGACGA GGTTCTGATC AAGATGGCAT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCATGC CCAGCATTGG 
AAGGGGGCCT CCAACTACGT GGCAAAACGC TACCTCGAGC CAGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAAGCG AAGCTCTGGG GGGAGGAGTA CAACCGGCAC 
AAGCCCCCCA AGCAGGTAGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACAA 
GGACAGCCCC TCTTCCACCT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTATAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC AGACTAACAC CACAGGCCTT CAGCCATTTC 
ACATTTGAAC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGCGACCTT 
TATACTGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGTATTTG CCAGAGCATG 
GGCCTTGCGC CCTTTGACCT CTCCCCACGG GAGCAGGATG CCGTGAACCA GAGCACCAAG 
CTGTTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTGGCAG CCGGCCCCCC TTGCTCCTTC GCCTGTCAGA GAACTCTGGG 
GATGAGAACA TGAGTGATGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCCACA 
CCACACAGCC AGAAGTTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAATGGCA ACCGAAGGCC TGAATCTGAT GAGGACAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCCAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAAAG GTTAAATGCT CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATCT GGCCATGGTA CGGTACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AATGGGATCA AGAGTCTGCC ATCTTCCATC TGGAGCATGC AGCCGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGGA GGAGACAGAA GAGAACAAGA CAAAAGGCTT TGATTACCTC 
CTGAAGGCCG CAGAAGCTGG TGACAGGCAG TCCATGATTT TAGTGGCTCG AGCTTTTGAC 
ACCGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT ACACTGGTAC 
AACACGGCCC TGGAGACGAC GGACTGCGAT GAAGGCGGGG AGTATGACGG AATACAGGAC 
GAGCCCCAGT ATGCACTGCT GGCCAGGGAG GCAGAGATGT TGCTCACCGG TGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAC TTGTACACCC AGGCAGCCGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTATGAGA AGGCGGAAGA GGCCTGGGCG 
CAAATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230219.1
CDS	717..2891
Translation	MADEDLIFRLEGVDGGGSSGAGRHGDSDTDSDDDEGYFICPITDDHMSNQNVNSKGQGYYNNLLKTECGSTGSPASSFHFKEAWKHAIEKAKHMPDPWAEFHLEDIATEHATRHRYNAVTGEWLKDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQHWKGASNYVAKRYLEPVDRSVYFEDVQLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRQGQPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTEKGTDFGDGNLGVRGMALFFYSHACNRICQSMGLAPFDLSPREQDAVNQSTKLLQSAKTILRGTEEKCGSPRIRTLSGSRPPLLLRLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFGDLDNMGPRDHDRMDNHRDSENSGDSGYPSEKRSDLDDPEPREHGHSNGNRRPESDEDSLGSSGRVCVETWNLLNPSRLHLPRPSAVALEVQRLNALDLGRKIGKSVLGKVHLAMVRYHEGGRFCEKDEEWDQESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLEETEENKTKGFDYLLKAAEAGDRQSMILVARAFDTGLNLSPDRCQDWSEALHWYNTALETTDCDEGGEYDGIQDEPQYALLAREAEMLLTGGFGLDKNPQRSGDLYTQAAEAAMEAMKGRLANQYYEKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_006230157.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230157.3

ORF Insert Sequence:

ATGGCAGACG AAGACCTCAT CTTCCGCCTG GAAGGTGTTG ACGGTGGCGG GTCCTCCGGA 
GCTGGCCGCC ATGGGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA CTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAACCAG AATGTCAACT CCAAAGGCCA GGGCTACTAC 
AACAACCTAC TGAAAACGGA GTGTGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAGGCCT GGAAGCATGC GATCGAGAAG GCCAAGCACA TGCCCGACCC CTGGGCTGAA 
TTCCATCTGG AGGACATCGC CACAGAGCAT GCTACTCGGC ACAGGTACAA TGCTGTCACC 
GGGGAGTGGC TGAAAGACGA GGTTCTGATC AAGATGGCAT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCATGC CCAGCATTGG 
AAGGGGGCCT CCAACTACGT GGCAAAACGC TACCTCGAGC CAGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAAGCG AAGCTCTGGG GGGAGGAGTA CAACCGGCAC 
AAGCCCCCCA AGCAGGTAGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACAA 
GGACAGCCCC TCTTCCACCT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTATAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC AGACTAACAC CACAGGCCTT CAGCCATTTC 
ACATTTGAAC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGCGACCTT 
TATACTGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGTATTTG CCAGAGCATG 
GGCCTTGCGC CCTTTGACCT CTCCCCACGG GAGCAGGATG CCGTGAACCA GAGCACCAAG 
CTGTTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTGGCAG CCGGCCCCCC TTGCTCCTTC GCCTGTCAGA GAACTCTGGG 
GATGAGAACA TGAGTGATGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCCACA 
CCACACAGCC AGAAGTTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAATGGCA ACCGAAGGCC TGAATCTGAT GAGGACAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCCAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAAAG GTTAAATGCT CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATCT GGCCATGGTA CGGTACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AATGGGATCA AGAGTCTGCC ATCTTCCATC TGGAGCATGC AGCCGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGGA GGAGACAGAA GAGAACAAGA CAAAAGGCTT TGATTACCTC 
CTGAAGGCCG CAGAAGCTGG TGACAGGCAG TCCATGATTT TAGTGGCTCG AGCTTTTGAC 
ACCGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT ACACTGGTAC 
AACACGGCCC TGGAGACGAC GGACTGCGAT GAAGGCGGGG AGTATGACGG AATACAGGAC 
GAGCCCCAGT ATGCACTGCT GGCCAGGGAG GCAGAGATGT TGCTCACCGG TGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAC TTGTACACCC AGGCAGCCGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTATGAGA AGGCGGAAGA GGCCTGGGCG 
CAAATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heat shock protein 70 protects cardiomyocytes through suppressing SUMOylation and nucleus translocation of phosphorylated eukaryotic elongation factor 2 during myocardial ischemia and reperfusion.
Apoptosis : an international journal on programmed cell death22(5)608-625(2017 May)
Zhang C,Liu X,Miao J,Wang S,Wu L,Yan D,Li J,Guo W,Wu X,Shen A

eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.
Cerebral cortex (New York, N.Y. : 1991)27(3)2226-2248(2017 Mar)
Heise C,Taha E,Murru L,Ponzoni L,Cattaneo A,Guarnieri FC,Montani C,Mossa A,Vezzoli E,Ippolito G,Zapata J,Barrera I,Ryazanov AG,Cook J,Poe M,Stephen MR,Kopanitsa M,Benfante R,Rusconi F,Braida D,Francolini M,Proud CG,Valtorta F,Passafaro M,Sala M,Bachi A,Verpelli C,Rosenblum K,Sala C

Intrauterine growth restriction inhibits expression of eukaryotic elongation factor 2 kinase, a regulator of protein translation.
Physiological genomics48(8)616-25(2016 Aug)
McKnight RA,Yost CC,Zinkhan EK,Fu Q,Callaway CW,Fung CM

Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes.
Genome biology1690(2015 May)
Andreev DE,O'Connor PB,Zhdanov AV,Dmitriev RI,Shatsky IN,Papkovsky DB,Baranov PV

Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation.
American journal of physiology. Heart and circulatory physiology305(5)H756-68(2013 Sep)
Usui T,Okada M,Hara Y,Yamawaki H

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Eef2k cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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