Acsl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acsl1
Entrez Gene ID	25288
Full Name	acyl-CoA synthetase long-chain family member 1
Synonyms	ACS,Acas,COAA,Facl2
General protein information	Preferred Names acyl-CoA synthetase long-chain family member 1 Names long-chain-fatty-acid--CoA ligase 1 Acyl CoA synthetase, long chain LACS 1 fatty acid Coenzyme A ligase, long chain 2 long-chain acyl-CoA synthetase 1 long-chain-fatty-acid--CoA ligase, liver isozyme
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q11
Summary	catalyzes the ligation of long chain fatty acids with coenzyme A to produce long chain acyl-CoAs; plays a role in lipid biosynthesis and fatty acid metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008771254.1	XP_008769476.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_006253126.2	XP_006253188.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_006253123.1	XP_006253185.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
NM_012820.1	NP_036952.1	long-chain-fatty-acid--CoA ligase 1
XM_006253127.1	XP_006253189.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_006253124.2	XP_006253186.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_006253122.1	XP_006253184.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_006253125.3	XP_006253187.1	long-chain-fatty-acid--CoA ligase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACSL1	NP_001273639.1
Mus musculus (house mouse)	Acsl1	NP_032007.2
	ACSL1	XP_001083742.2
Gallus gallus (chicken)	ACSL1	NP_001012596.1
Arabidopsis thaliana (thale cress)	LACS7	NP_198112.2
Xenopus tropicalis (tropical clawed frog)	acsl1	NP_001006830.1
Canis lupus familiaris (dog)	ACSL1	XP_005630044.1
Pan troglodytes (chimpanzee)	ACSL1	XP_003310629.1
Bos taurus (cattle)	ACSL1	NP_001069553.1
Rattus norvegicus (Norway rat)	Acsl1	NP_036952.1

Gene Ontology
Bibliography

Related articles in PubMed

Long-chain acyl-CoA synthetase isoforms differ in preferences for eicosanoid species and long-chain fatty acids.
Klett EL, Chen S, Yechoor A, Lih FB, Coleman RA
Journal of lipid research58(5)884-894(2017 May)

Long-chain bases of sphingolipids are transported into cells via the acyl-CoA synthetases.
Narita T, Naganuma T, Sase Y, Kihara A
Scientific reports625469(2016 05)

Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function.
Grevengoed TJ, Martin SA, Katunga L, Cooper DE, Anderson EJ, Murphy RC, Coleman RA
Journal of lipid research56(8)1572-82(2015 Aug)

Role of long-chain acyl-coenzyme A synthetases in the regulation of arachidonic acid metabolism in interleukin 1?-stimulated rat fibroblasts.
Kuwata H, Yoshimura M, Sasaki Y, Yoda E, Nakatani Y, Kudo I, Hara S
Biochimica et biophysica acta1841(1)44-53(2014 Jan)

Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
Ohkuni A, Ohno Y, Kihara A
Biochemical and biophysical research communications442(3-4)195-201(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function
Title: Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function.

Acsl isozymes play distinct roles in the control of AA remodeling in rat fibroblasts: Acsl4 acts as the first step of enzyme for AA remodeling following IL-1beta stimulation, and Acsl1 is involved in the maintenance of some AA-containing PC species.
Title: Role of long-chain acyl-coenzyme A synthetases in the regulation of arachidonic acid metabolism in interleukin 1?-stimulated rat fibroblasts.

rat long chain acyl-coa synthetase 1 has a role in fatty acid metabolism in rat primary hepatocytes
Title: Overexpression of rat long chain acyl-coa synthetase 1 alters fatty acid metabolism in rat primary hepatocytes.

Acyl-CoA synthetase 2 has a role in fatty acid internalization and neurite outgrowth
Title: Acyl-CoA synthetase 2 overexpression enhances fatty acid internalization and neurite outgrowth.

Long-chain acyl-CoenzymeA synthetase-2 expression is significantly upregulated in distinct regions of the frontal cortex in learned helpless rats relative to controls
Title: Long-chain acyl-CoenzymeA synthetase-2 mRNA: increased cerebral cortex expression in an animal model of depression.

The following Acsl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acsl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01027	NM_012820.1 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01027	XM_006253124.2 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01027	XM_008771254.1 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01027	XM_006253126.2 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01027	XM_006253123.1 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01027	XM_006253127.1 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01027	XM_006253122.1 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01027	XM_006253125.3 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acsl1 ( NM_012820.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036952.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), mRNA.

Price & Availability↑

CloneID	ORa01027
Clone ID Related Accession (Same CDS sequence)	XM_008771254.1 , XM_006253126.2 , XM_006253123.1 , NM_012820.1 , XM_006253127.1 , XM_006253124.2 , XM_006253122.1 , XM_006253125.3
Accession Version	NM_012820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D90109.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D90109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036952.1
CDS	14..2113
Translation	MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Target ORF information:

RefSeq Version	NM_012820.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012820.1

ORF Insert Sequence:

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl1 ( XM_008771254.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769476.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa01027
Clone ID Related Accession (Same CDS sequence)	XM_008771254.1 , XM_006253126.2 , XM_006253123.1 , NM_012820.1 , XM_006253127.1 , XM_006253124.2 , XM_006253122.1 , XM_006253125.3
Accession Version	XM_008771254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769476.1
CDS	40..2139
Translation	MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Target ORF information:

RefSeq Version	XM_008771254.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771254.1

ORF Insert Sequence:

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl1 ( XM_006253126.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253188.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa01027
Clone ID Related Accession (Same CDS sequence)	XM_008771254.1 , XM_006253126.2 , XM_006253123.1 , NM_012820.1 , XM_006253127.1 , XM_006253124.2 , XM_006253122.1 , XM_006253125.3
Accession Version	XM_006253126.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253188.1
CDS	155..2254
Translation	MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Target ORF information:

RefSeq Version	XM_006253126.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253126.2

ORF Insert Sequence:

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl1 ( XM_006253123.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253185.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01027
Clone ID Related Accession (Same CDS sequence)	XM_008771254.1 , XM_006253126.2 , XM_006253123.1 , NM_012820.1 , XM_006253127.1 , XM_006253124.2 , XM_006253122.1 , XM_006253125.3
Accession Version	XM_006253123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253185.1
CDS	215..2314
Translation	MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Target ORF information:

RefSeq Version	XM_006253123.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253123.1

ORF Insert Sequence:

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl1 ( XM_006253127.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253189.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa01027
Clone ID Related Accession (Same CDS sequence)	XM_008771254.1 , XM_006253126.2 , XM_006253123.1 , NM_012820.1 , XM_006253127.1 , XM_006253124.2 , XM_006253122.1 , XM_006253125.3
Accession Version	XM_006253127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253189.1
CDS	105..2204
Translation	MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Target ORF information:

RefSeq Version	XM_006253127.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253127.1

ORF Insert Sequence:

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl1 ( XM_006253124.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253186.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa01027
Clone ID Related Accession (Same CDS sequence)	XM_008771254.1 , XM_006253126.2 , XM_006253123.1 , NM_012820.1 , XM_006253127.1 , XM_006253124.2 , XM_006253122.1 , XM_006253125.3
Accession Version	XM_006253124.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006253124.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253186.1
CDS	134..2233
Translation	MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Target ORF information:

RefSeq Version	XM_006253124.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253124.2

ORF Insert Sequence:

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl1 ( XM_006253122.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253184.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa01027
Clone ID Related Accession (Same CDS sequence)	XM_008771254.1 , XM_006253126.2 , XM_006253123.1 , NM_012820.1 , XM_006253127.1 , XM_006253124.2 , XM_006253122.1 , XM_006253125.3
Accession Version	XM_006253122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253184.1
CDS	674..2773
Translation	MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Target ORF information:

RefSeq Version	XM_006253122.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253122.1

ORF Insert Sequence:

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl1 ( XM_006253125.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253187.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X6, mRNA.

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CloneID	ORa01027
Clone ID Related Accession (Same CDS sequence)	XM_008771254.1 , XM_006253126.2 , XM_006253123.1 , NM_012820.1 , XM_006253127.1 , XM_006253124.2 , XM_006253122.1 , XM_006253125.3
Accession Version	XM_006253125.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253187.1
CDS	296..2395
Translation	MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Target ORF information:

RefSeq Version	XM_006253125.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 1 (Acsl1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253125.3

ORF Insert Sequence:

ATGGAAGTCC ACGAATTGTT CCGGTATTTT CGAATGCCAG AGCTGATTGA CATTCGGCAG 
TACGTGCGTA CCCTTCCAAC CAACACACTC ATGGGCTTCG GGGCTTTTGC AGCGCTCACC 
ACCTTCTGGT ATGCCACCCG GCCGAAGGCC CTGAAGCCAC CATGTGATCT GTCCATGCAG 
TCTGTGGAAG TAACGGGTAC TACTGAGGGT GTCCGAAGAT CAGCAGTCCT TGAGGACGAC 
AAGCTCTTGC TGTACTACTA CGACGATGTC AGAACGATGT ACGATGGCTT CCAGAGGGGG 
ATTCAGGTGT CAAATGATGG CCCTTGTTTA GGTTCTAGAA AGCCAAACCA GCCATATGAG 
TGGATTTCTT ACAAACAGGT TGCAGAAATG GCTGAGTGCA TAGGCTCGGC GCTGATCCAG 
AAGGGTTTCA AACCTTGCTC AGAGCAGTTC ATCGGCATCT TTTCTCAGAA CAGACCTGAG 
TGGGTGACCA TCGAGCAGGG GTGCTTCACT TACTCCATGG TGGTTGTTCC GCTCTATGAC 
ACGCTTGGAA CCGACGCCAT CACCTACATA GTGAACAAAG CTGAACTCTC TGTGATTTTT 
GCTGACAAGC CAGAAAAAGC CAAACTCTTA TTAGAAGGTG TAGAAAATAA GTTAACACCA 
TGCCTTAAAA TCATAGTCAT CATGGACTCC TACGACAATG ATCTGGTGGA ACGCGGCCAG 
AAGTGTGGGG TGGAAATCAT CGGCCTAAAA GCTCTGGAGG ATCTTGGAAG AGTGAACAGA 
ACGAAACCCA AGCCTCCAGA ACCTGAAGAT CTTGCGATAA TCTGTTTCAC AAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGGTCACC CACCAAAACA TTATGAACGA TTGCTCCGGT 
TTTATAAAAG CGACGGAGAG TGCATTCATC GCTTCCCCAG AGGATGTTCT GATATCTTTC 
TTGCCTCTCG CCCATATGTT TGAGACCGTT GTAGAGTGTG TAATGCTATG TCATGGAGCT 
AAGATAGGAT TTTTCCAAGG AGACATCAGG CTGCTTATGG ATGACCTCAA GGTGCTTCAG 
CCTACCATCT TCCCTGTGGT TCCGAGACTG CTAAACCGGA TGTTTGACAG AATTTTTGGA 
CAAGCAAACA CGTCAGTGAA GCGATGGCTG TTGGATTTTG CCTCCAAAAG GAAAGAGGCG 
GAGCTTCGCA GTGGCATCGT CAGAAACAAC AGCCTGTGGG ATAAACTCAT CTTTCACAAG 
ATACAGTCGA GCCTGGGCGG GAAAGTCCGG CTGATGATCA CAGGAGCAGC CCCAGTGTCT 
GCCACAGTGC TGACGTTTCT GAGGGCGGCT CTCGGCTGTC AGTTCTATGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG TTGCTGCCTG AGCTTGCCTG GAGACTGGAC AGCAGGCCAT 
GTTGGTGCCC CCATGCCTTG CAATTATATC AAGCTTGTAG ATGTGGAAGA TATGAATTAC 
CAGGCTGCCA AGGGCGAGGG TGAGGTGTGT GTGAAAGGGG CAAATGTGTT CAAAGGCTAC 
TTGAAAGACC CAGCAAGAAC AGCTGAAGCC CTAGATAAAG ATGGTTGGTT ACACACGGGG 
GACATTGGAA AATGGCTGCC AAATGGCACC TTGAAGATTA TCGACAGGAA AAAGCACATA 
TTTAAACTAG CCCAAGGAGA GTACATAGCT CCAGAAAAGA TCGAAAATAT CTACCTGCGG 
AGCGAAGCCG TGGCCCAGGT GTTTGTCCAT GGAGAAAGCT TGCAGGCCTT TCTCATAGCC 
ATTGTAGTAC CTGATGTGGA GATCCTACCT TCCTGGGCAC AGAAGAGAGG ATTTCAAGGG 
TCCTTTGAAG AACTGTGCAG GAACAAGGAT ATCAATAAAG CTATCCTGGA AGACATGGTG 
AAACTTGGGA AGAATGCTGG TTTGAAGCCA TTTGAACAGG TCAAAGGCAT TGCTGTGCAC 
CCGGAATTAT TTTCTATTGA CAATGGCCTC CTGACTCCAA CACTGAAGGC AAAAAGGCCA 
GAGCTGCGGA ACTATTTCAG GTCACAGATA GATGAACTGT ACTCCACCAT CAAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Long-chain acyl-CoA synthetase isoforms differ in preferences for eicosanoid species and long-chain fatty acids.
Journal of lipid research58(5)884-894(2017 May)
Klett EL,Chen S,Yechoor A,Lih FB,Coleman RA

Long-chain bases of sphingolipids are transported into cells via the acyl-CoA synthetases.
Scientific reports625469(2016 05)
Narita T,Naganuma T,Sase Y,Kihara A

Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function.
Journal of lipid research56(8)1572-82(2015 Aug)
Grevengoed TJ,Martin SA,Katunga L,Cooper DE,Anderson EJ,Murphy RC,Coleman RA

Role of long-chain acyl-coenzyme A synthetases in the regulation of arachidonic acid metabolism in interleukin 1?-stimulated rat fibroblasts.
Biochimica et biophysica acta1841(1)44-53(2014 Jan)
Kuwata H,Yoshimura M,Sasaki Y,Yoda E,Nakatani Y,Kudo I,Hara S

Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
Biochemical and biophysical research communications442(3-4)195-201(2013 Dec)
Ohkuni A,Ohno Y,Kihara A

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Acsl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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