Arhgap4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arhgap4
Entrez Gene ID	246249
Full Name	Rho GTPase activating protein 4
General protein information	Preferred Names Rho GTPase activating protein 4 Names rho GTPase-activating protein 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq37

mRNA and Protein(s)

mRNA	Protein	Name
NM_144740.3	NP_653341.2	rho GTPase-activating protein 4
XM_006229547.3	XP_006229609.1	rho GTPase-activating protein 4 isoform X3
XM_006229546.3	XP_006229608.1	rho GTPase-activating protein 4 isoform X2
XM_006229548.3	XP_006229610.1	rho GTPase-activating protein 4 isoform X4
XM_006229545.3	XP_006229607.1	rho GTPase-activating protein 4 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARHGAP4	XP_549365.3
	ARHGAP4	XP_002806496.1
Danio rerio (zebrafish)	arhgap4b	XP_689729.3
Danio rerio (zebrafish)	arhgap4a	NP_956738.1
Homo sapiens (human)	ARHGAP4	NP_001158213.1
Pan troglodytes (chimpanzee)	ARHGAP4	XP_003317822.1
Mus musculus (house mouse)	Arhgap4	NP_619536.2
Xenopus tropicalis (tropical clawed frog)	arhgap4	NP_001123805.1
Bos taurus (cattle)	ARHGAP4	NP_001096010.1
Rattus norvegicus (Norway rat)	Arhgap4	NP_653341.2

Gene Ontology
Bibliography

Related articles in PubMed

The Neuronal Migration Factor srGAP2 Achieves Specificity in Ligand Binding through a Two-Component Molecular Mechanism.
Guez-Haddad J, Sporny M, Sasson Y, Gevorkyan-Airapetov L, Lahav-Mankovski N, Margulies D, Radzimanowski J, Opatowsky Y
Structure (London, England : 1993)23(11)1989-2000(2015 Nov)

ARHGAP4 is a novel RhoGAP that mediates inhibition of cell motility and axon outgrowth.
Vogt DL, Gray CD, Young WS 3rd, Orellana SA, Malouf AT
Molecular and cellular neurosciences36(3)332-42(2007 Nov)

Cloning of rat ARHGAP4/C1, a RhoGAP family member expressed in the nervous system that colocalizes with the Golgi complex and microtubules.
Foletta VC, Brown FD, Young WS 3rd
Brain research. Molecular brain research107(1)65-79(2002 Oct)

RefSeq and LocusLink: NCBI gene-centered resources.
Pruitt KD, Maglott DR
Nucleic acids research29(1)137-40(2001 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These observations suggest that ARHGAP4 can act as a potent inhibitor of cell and axon motility when it is localized to the leading edge of motile cells and axons.
Title: ARHGAP4 is a novel RhoGAP that mediates inhibition of cell motility and axon outgrowth.

ARHGAP4 occured in the hippocampus, ventral region of the brainstem and striatum, and in cerebellar granule cells. Subcellularly, ARHGAP4 localized to the Golgi complex and could redistribute to the microtubules, for example during mitosis.
Title: Cloning of rat ARHGAP4/C1, a RhoGAP family member expressed in the nervous system that colocalizes with the Golgi complex and microtubules.

The following Arhgap4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgap4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02439	NM_144740.3 Latest version!	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$566.30 $809.00
ORa23004	XM_006229545.3 Latest version!	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa23005	XM_006229546.3 Latest version!	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa23006	XM_006229547.3 Latest version!	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa23007	XM_006229548.3 Latest version!	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arhgap4 ( NM_144740.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_653341.2 Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), mRNA.

Price & Availability↑

CloneID	ORa02439
Clone ID Related Accession (Same CDS sequence)	NM_144740.3
Accession Version	NM_144740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	rho GTPase-activating protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF401635.1 and CO572120.1. On Nov 1, 2005 this sequence version replaced NM_144740.2. ##Evidence-Data-START## Transcript exon combination :: AF401635.1, AY742900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689603 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGCG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAG TAAGGACCTG GTGCAGCAAC TGCAGGATGA GCTCCTGGAG 
GTGGTCTCAG AACTCCAGAC ACAGGGCTAC CTTGCCTGGG CCTCCCTGCA GACCAAGAAG 
ACGTACCATG TATACCACTT GGAGAGCATG AATGCTGAGA CTAAACTTCG GGAAGCTGAG 
AGGCAAGAGG AGAAGCGGTC AGGCAAGAGT GCCTTGCCCA CCACTACTGC TGCCTCTGCC 
ACCACCACCA TCACCATTGA GACAGAGCTC CACCGAAAGG GCTCTCTCAA GAAAGGAGGG 
CGGCTAGTGG AGAAGCGTCA GGCCAAGTTC TTGGAACATA AACTTAAATG CACCAAGGCC 
CGAAATGAGT ACCTGCTCAG CCTGGCCAGT GTCAATGCTG CTGTCAGCAA CTACTACTTA 
CACGATATCT TGGACCTCAT GGATTGCTGT GACACAGGTT TCCACTTGGC CCTGGAGCAG 
GCCCTCCGAA GCTACACAGC CGCTGAAAGC CGCACCCAAA CCTCTCACAT GCAGGGTTTA 
GGCAGTCTGG AAGAGGCTCT AGAGGCCTTG GATCCTCCAG GGGACAAAGC CAAAGTGCTT 
GAGGTTCATG CCATGGCCTT CTGTCCCCCT CTACGTTTTG ACTACCAGCC CCATGAAGGT 
GATGAGGTGG CTGAGATCCA GGTTGAGATG GAACTTCGGG ATGAGATTTT GCCCAGAGCC 
CAAAACATCC AAAGTCGCCT GGACCAAAAG ACCATTGAGA CAGAAGAGGT GAATAAGACA 
CTGAAGGCAA CACTTCAGGC TCTGCTAGAG GTGGTGGCAT CAGAGGATGG AGATATACTG 
GACTCTTTGC AGGCCAGCCC CTCCACTGAG TCCCTCAAGT CTACAAGCTC AGACCCAGGC 
ACCCGACAGG CAGGCCGCAG ACGGAACCAG CAGCAGGAGA CAGAAACCTT CTATATTACG 
AAGCTGCAGG AATATCTGAG TGGCCGGAGC ATCCTTGCCA AGCTGCAGGC CAAGCATGAA 
AAGCTCCAGG AAGCCATACA ACGAGGTAAC AAGGAAGAGC AAGAGACATC TTCTTGGACC 
CAATGCACAG AGAGAAAATT CCATAAGAGC CACCACCCCT ATCCTGGATC CCAGTGTACC 
CAGAGACTTT TTGGAGGTGA TCTAGAGAAG TTTATCCAGA GCACAGGCCA ACCTGTGCCC 
CTTGTGGTGA AGAGCTGTAT CCGCTTCATT AACCTCAATG GCCTGCAACA TGAAGGCATC 
TTCCGGGTAT CAGGTGCCCA GGCCCGGATA TTGGAGATCC GAGATGCCTT TGAAAGAGGG 
GAGGACCCTC TGGTGGAAGG CTGTACTGCC CATGACCTAG ACTCAGTGGC TGGGGTACTA 
AAGCTGTACT TCCGGAGCCT GGAGCCCCCA CTGTTCCCCT TGGACATGTT TAATGAGCTG 
CTGGCTTCAG CAGAACTGGA AGTTGTGGGT GAGCGGGTAG AGCTTGTGAG CCATCTACTA 
TCCAGACTAC CCAGACCTGT GCTAGTGGTC TTGCGATATC TCTTCACCTT CCTCAACCAC 
CTGGCCCAGT ACAGCGATGA GAACATGATG GACTCCTACA ACTTGGCTGT ATGTTTTGGG 
CCCACGCTGT TGCCTGTGCC TGCTGGGCAG GACCCTGTAG CCTTGCAGGG CCGAGTGAAC 
CAGCTGGTCC AGACCCTTAT CCTGCAACCA GCATGGGTTT TCCCACCCAC AGCCATGCTA 
CCAGGTCCCA TCTATGAGAA GTGCATGGCA CCACCTTCTG CCAGCTGCCT GGGGGATGGC 
CAGTTGGAGA GCCTTGCTGG GGAGCCAGAG CTGGAATTGA AAACTGGGAC CAAAGCTCAG 
GAAGATGACT CCGAGGGAGT TGTGGAGGCT GTGGCCTGCT TTGCTTACAC TGGCCGCACG 
GCCCAGGAGC TGACATTCCA GCAAGGGGAT ACGCTGAGAT TGTATGCTCG GGCATCAAGT 
GACTGGTGGC GAGGGGAGCA TGCTGGTGTG CGGGGGCTCA TTCCCCATAA GTACATCACA 
CTGTCTGAGG GGGATGAGAA GCAGATGACT GGTTCAGGAT TACAGGCCAT GGGGGAATCT 
CTGAGCCATC CAGAAAGCGT CCTGACCTTG GAGTTTACCA ACCGACTGGA ACTAGGCACC 
CCACCTGAGG CTTTGGGCCC CTCTGGTCAC AGACGACACT GTTTGGTCCC TACCTCCCCT 
GAGCAACATG TGGAAATGGA TAAGGCTGTG GCGCAGAACA TGGATTCTGT GTTTAAGGAG 
CTCTTGGGAA AGGCTGCTGT CCGCCAGGGC CATGGGCTAG CATCTACAGC CTCCCCCAGT 
CTAGGAACTC GAAACCTGAA GCCCCTGGCC TGCAGCAGCT TCAGCAAAAA CAAAGTCTTC 
TCCCGGGGAC CTGGGGCTCC AGTTTCCCCC TCAGTTTCCC ATCCCCAGGG TCCAGATTCA 
ACTCGCAAGC CAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653341.2
CDS	99..2996
Translation	MAAHGKLRRERGLQPEYEAQVKVMRGQLSEQLHCLELQGELRRDLLLELAEFMRRRAEVELEYSRGLDKLAERFTSRSGRLGGSSREQQSFRKEPTLLSPLHCWAMLLEHTRQQSRESATLSEVLAGPLAQRLSHIAEDVGRIVKKSKDLVQQLQDELLEVVSELQTQGYLAWASLQTKKTYHVYHLESMNAETKLREAERQEEKRSGKSALPTTTAASATTTITIETELHRKGSLKKGGRLVEKRQAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLHDILDLMDCCDTGFHLALEQALRSYTAAESRTQTSHMQGLGSLEEALEALDPPGDKAKVLEVHAMAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDQKTIETEEVNKTLKATLQALLEVVASEDGDILDSLQASPSTESLKSTSSDPGTRQAGRRRNQQQETETFYITKLQEYLSGRSILAKLQAKHEKLQEAIQRGNKEEQETSSWTQCTERKFHKSHHPYPGSQCTQRLFGGDLEKFIQSTGQPVPLVVKSCIRFINLNGLQHEGIFRVSGAQARILEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLEPPLFPLDMFNELLASAELEVVGERVELVSHLLSRLPRPVLVVLRYLFTFLNHLAQYSDENMMDSYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLILQPAWVFPPTAMLPGPIYEKCMAPPSASCLGDGQLESLAGEPELELKTGTKAQEDDSEGVVEAVACFAYTGRTAQELTFQQGDTLRLYARASSDWWRGEHAGVRGLIPHKYITLSEGDEKQMTGSGLQAMGESLSHPESVLTLEFTNRLELGTPPEALGPSGHRRHCLVPTSPEQHVEMDKAVAQNMDSVFKELLGKAAVRQGHGLASTASPSLGTRNLKPLACSSFSKNKVFSRGPGAPVSPSVSHPQGPDSTRKPV

Target ORF information:

RefSeq Version	NM_144740.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144740.3

ORF Insert Sequence:

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGCG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAG TAAGGACCTG GTGCAGCAAC TGCAGGATGA GCTCCTGGAG 
GTGGTCTCAG AACTCCAGAC ACAGGGCTAC CTTGCCTGGG CCTCCCTGCA GACCAAGAAG 
ACGTACCATG TATACCACTT GGAGAGCATG AATGCTGAGA CTAAACTTCG GGAAGCTGAG 
AGGCAAGAGG AGAAGCGGTC AGGCAAGAGT GCCTTGCCCA CCACTACTGC TGCCTCTGCC 
ACCACCACCA TCACCATTGA GACAGAGCTC CACCGAAAGG GCTCTCTCAA GAAAGGAGGG 
CGGCTAGTGG AGAAGCGTCA GGCCAAGTTC TTGGAACATA AACTTAAATG CACCAAGGCC 
CGAAATGAGT ACCTGCTCAG CCTGGCCAGT GTCAATGCTG CTGTCAGCAA CTACTACTTA 
CACGATATCT TGGACCTCAT GGATTGCTGT GACACAGGTT TCCACTTGGC CCTGGAGCAG 
GCCCTCCGAA GCTACACAGC CGCTGAAAGC CGCACCCAAA CCTCTCACAT GCAGGGTTTA 
GGCAGTCTGG AAGAGGCTCT AGAGGCCTTG GATCCTCCAG GGGACAAAGC CAAAGTGCTT 
GAGGTTCATG CCATGGCCTT CTGTCCCCCT CTACGTTTTG ACTACCAGCC CCATGAAGGT 
GATGAGGTGG CTGAGATCCA GGTTGAGATG GAACTTCGGG ATGAGATTTT GCCCAGAGCC 
CAAAACATCC AAAGTCGCCT GGACCAAAAG ACCATTGAGA CAGAAGAGGT GAATAAGACA 
CTGAAGGCAA CACTTCAGGC TCTGCTAGAG GTGGTGGCAT CAGAGGATGG AGATATACTG 
GACTCTTTGC AGGCCAGCCC CTCCACTGAG TCCCTCAAGT CTACAAGCTC AGACCCAGGC 
ACCCGACAGG CAGGCCGCAG ACGGAACCAG CAGCAGGAGA CAGAAACCTT CTATATTACG 
AAGCTGCAGG AATATCTGAG TGGCCGGAGC ATCCTTGCCA AGCTGCAGGC CAAGCATGAA 
AAGCTCCAGG AAGCCATACA ACGAGGTAAC AAGGAAGAGC AAGAGACATC TTCTTGGACC 
CAATGCACAG AGAGAAAATT CCATAAGAGC CACCACCCCT ATCCTGGATC CCAGTGTACC 
CAGAGACTTT TTGGAGGTGA TCTAGAGAAG TTTATCCAGA GCACAGGCCA ACCTGTGCCC 
CTTGTGGTGA AGAGCTGTAT CCGCTTCATT AACCTCAATG GCCTGCAACA TGAAGGCATC 
TTCCGGGTAT CAGGTGCCCA GGCCCGGATA TTGGAGATCC GAGATGCCTT TGAAAGAGGG 
GAGGACCCTC TGGTGGAAGG CTGTACTGCC CATGACCTAG ACTCAGTGGC TGGGGTACTA 
AAGCTGTACT TCCGGAGCCT GGAGCCCCCA CTGTTCCCCT TGGACATGTT TAATGAGCTG 
CTGGCTTCAG CAGAACTGGA AGTTGTGGGT GAGCGGGTAG AGCTTGTGAG CCATCTACTA 
TCCAGACTAC CCAGACCTGT GCTAGTGGTC TTGCGATATC TCTTCACCTT CCTCAACCAC 
CTGGCCCAGT ACAGCGATGA GAACATGATG GACTCCTACA ACTTGGCTGT ATGTTTTGGG 
CCCACGCTGT TGCCTGTGCC TGCTGGGCAG GACCCTGTAG CCTTGCAGGG CCGAGTGAAC 
CAGCTGGTCC AGACCCTTAT CCTGCAACCA GCATGGGTTT TCCCACCCAC AGCCATGCTA 
CCAGGTCCCA TCTATGAGAA GTGCATGGCA CCACCTTCTG CCAGCTGCCT GGGGGATGGC 
CAGTTGGAGA GCCTTGCTGG GGAGCCAGAG CTGGAATTGA AAACTGGGAC CAAAGCTCAG 
GAAGATGACT CCGAGGGAGT TGTGGAGGCT GTGGCCTGCT TTGCTTACAC TGGCCGCACG 
GCCCAGGAGC TGACATTCCA GCAAGGGGAT ACGCTGAGAT TGTATGCTCG GGCATCAAGT 
GACTGGTGGC GAGGGGAGCA TGCTGGTGTG CGGGGGCTCA TTCCCCATAA GTACATCACA 
CTGTCTGAGG GGGATGAGAA GCAGATGACT GGTTCAGGAT TACAGGCCAT GGGGGAATCT 
CTGAGCCATC CAGAAAGCGT CCTGACCTTG GAGTTTACCA ACCGACTGGA ACTAGGCACC 
CCACCTGAGG CTTTGGGCCC CTCTGGTCAC AGACGACACT GTTTGGTCCC TACCTCCCCT 
GAGCAACATG TGGAAATGGA TAAGGCTGTG GCGCAGAACA TGGATTCTGT GTTTAAGGAG 
CTCTTGGGAA AGGCTGCTGT CCGCCAGGGC CATGGGCTAG CATCTACAGC CTCCCCCAGT 
CTAGGAACTC GAAACCTGAA GCCCCTGGCC TGCAGCAGCT TCAGCAAAAA CAAAGTCTTC 
TCCCGGGGAC CTGGGGCTCC AGTTTCCCCC TCAGTTTCCC ATCCCCAGGG TCCAGATTCA 
ACTCGCAAGC CAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap4 ( XM_006229545.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229607.1 Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23004
Clone ID Related Accession (Same CDS sequence)	XM_006229545.3
Accession Version	XM_006229545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rho GTPase-activating protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229545.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGGG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAG TAAGGACCTG GTGCAGCAAC TGCAGGATGA GCTCCTGGAG 
GTGGTCTCAG AACTCCAGAC AACCAAGAAG ACGTACCATG TATACCACTT GGAGAGCATG 
AATGCTGAGA CTAAACTTCG GGAAGCTGAG AGGCAAGAGG AGAAGCGGTC AGGCAAGAGT 
GCCTTGCCCA CCACTACTGC TGCCTCTGCC ACCACCACCA TCACCATTGA GACAGAGCTC 
CACCGAAAGG GCTCTCTCAA GAAAGGAGGG CGGCTAGTGG AGAAGCGTCA GGCCAAGTTC 
TTGGAACATA AACTTAAATG CACCAAGGCC CGAAATGAGT ACCTGCTCAG CCTGGCCAGT 
GTCAATGCTG CTGTCAGCAA CTACTACTTA CACGATATCT TGGACCTCAT GGATTGCTGT 
GACACAGGTT TCCACTTGGC CCTGGAGCAG GCCCTCCGAA GCTACACAGC CGCTGAAAGC 
CGCACCCAAA CCTCTCACAT GCAGGGTTTA GGCAGTCTGG AAGAGGCTCT AGAGGCCTTG 
GATCCTCCAG GGGACAAAGC CAAAGTGCTT GAGGTTCATG CCATGGCCTT CTGTCCCCCT 
CTACGTTTTG ACTACCAGCC CCATGAAGGT GATGAGGTGG CTGAGATCCA GGTTGAGATG 
GAACTTCGGG ATGAGATTTT GCCCAGAGCC CAAAACATCC AAAGTCGCCT GGACCAAAAG 
ACCATTGAGA CAGAAGAGGT GAATAAGACA CTGAAGGCAA CACTTCAGGC TCTGCTAGAG 
GTGGTGGCAT CAGAGGATGG AGATATACTG GACTCTTTGC AGGCCAGCCC CTCCACTGAG 
TCCCTCAAGT CTACAAGCTC AGACCCAGGC ACCCGACAGG CAGGCCGCAG ACGGAACCAG 
CAGCAGGAGA CAGAAACCTT CTATATTACG AAGCTGCAGG AATATCTGAG TGGCCGGAGC 
ATCCTTGCCA AGCTGCAGGC CAAGCATGAA AAGCTCCAGG AAGCCATACA ACGAGGTAAC 
AAGGAAGAGC AAGAGACATC TTCTTGGACC CAATGCACAG AGAGAAAATT CCATAAGAGC 
CACCACCCCT ATCCTGGATC CCAGTGTACC CAGAGACTTT TTGGAGGTGA TCTAGAGAAG 
TTTATCCAGA GCACAGGCCA ACCTGTGCCC CTTGTGGTGA AGAGCTGTAT CCGCTTCATT 
AACCTCAATG GCCTGCAACA TGAAGGCATC TTCCGGGTAT CAGGTGCCCA GGCCCGGATA 
TTGGAGATCC GAGATGCCTT TGAAAGAGGG GAGGACCCTC TGGTGGAAGG CTGTACTGCC 
CATGACCTAG ACTCAGTGGC TGGGGTACTA AAGCTGTACT TCCGGAGCCT GGAGCCCCCA 
CTGTTCCCCT TGGACATGTT TAATGAGCTG CTGGCTTCAG CAGAACTGGA AGTTGTGGGT 
GAGCGGGTAG AGCTTGTGAG CCATCTACTA TCCAGACTAC CCAGACCTGT GCTAGTGGTC 
TTGCGATATC TCTTCACCTT CCTCAACCAC CTGGCCCAGT ACAGCGATGA GAACATGATG 
GACTCCTACA ACTTGGCTGT ATGTTTTGGG CCCACGCTGT TGCCTGTGCC TGCTGGGCAG 
GACCCTGTAG CCTTGCAGGG CCGAGTGAAC CAGCTGGTCC AGACCCTTAT CCTGCAACCA 
GCATGGGTTT TCCCACCCAC AGCCATGCTA CCAGGTCCCA TCTATGAGAA GTGCATGGCA 
CCACCTTCTG CCAGCTGCCT GGGGGATGGC CAGTTGGAGA GCCTTGCTGG GGAGCCAGAG 
CTGGAATTGA AAACTGGGAC CAAAGCTCAG GAAGATGACT CCGAGGGAGT TGTGGAGGCT 
GTGGCCTGCT TTGCTTACAC TGGCCGCACG GCCCAGGAGC TGACATTCCA GCAAGGGGAT 
ACGCTGAGAT TGTATGCTCG GGCATCAAGT GACTGGTGGC GAGGGGAGCA TGCTGGTGTG 
CGGGGGCTCA TTCCCCATAA GTACATCACA CTGTCTGAGG GGGATGAGAA GCAGATGACT 
GGTTCAGGAT TACAGGCCAT GGGGGAATCT CTGAGCCATC CAGAAAGCGT CCTGACCTTG 
GAGTTTACCA ACCGACTGGA ACTAGGCACC CCACCTGAGG CTTTGGGCCC CTCTGGTCAC 
AGACGACACT GTTTGGTCCC TACCTCCCCT GAGCAACATG TGGAAATGGA TAAGGCTGTG 
GCGCAGAACA TGGATTCTGT GTTTAAGGAG CTCTTGGGAA AGGCTGCTGT CCGCCAGGGC 
CATGGGCTAG CATCTACAGC CTCCCCCAGT CTAGGAACTC GAAACCTGAA GCCCCTGGCC 
TGCAGCAGCT TCAGCAAAAA CAAAGTCTTC TCCCGGGGAC CTGGGGCTCC AGTTTCCCCC 
TCAGTTTCCC ATCCCCAGGG TCCAGATTCA ACTCGCAAGC CAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229607.1
CDS	895..3762
Translation	MAAHGKLRRERGLQPEYEAQVKVMRGQLSEQLHCLELQGELRRDLLLELAEFMRRRAEVELEYSRGLDKLAERFTSRSGRLGGSSREQQSFRKEPTLLSPLHCWAMLLEHTRQQSRESATLSEVLAGPLAQRLSHIAEDVGRIVKKSKDLVQQLQDELLEVVSELQTTKKTYHVYHLESMNAETKLREAERQEEKRSGKSALPTTTAASATTTITIETELHRKGSLKKGGRLVEKRQAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLHDILDLMDCCDTGFHLALEQALRSYTAAESRTQTSHMQGLGSLEEALEALDPPGDKAKVLEVHAMAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDQKTIETEEVNKTLKATLQALLEVVASEDGDILDSLQASPSTESLKSTSSDPGTRQAGRRRNQQQETETFYITKLQEYLSGRSILAKLQAKHEKLQEAIQRGNKEEQETSSWTQCTERKFHKSHHPYPGSQCTQRLFGGDLEKFIQSTGQPVPLVVKSCIRFINLNGLQHEGIFRVSGAQARILEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLEPPLFPLDMFNELLASAELEVVGERVELVSHLLSRLPRPVLVVLRYLFTFLNHLAQYSDENMMDSYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLILQPAWVFPPTAMLPGPIYEKCMAPPSASCLGDGQLESLAGEPELELKTGTKAQEDDSEGVVEAVACFAYTGRTAQELTFQQGDTLRLYARASSDWWRGEHAGVRGLIPHKYITLSEGDEKQMTGSGLQAMGESLSHPESVLTLEFTNRLELGTPPEALGPSGHRRHCLVPTSPEQHVEMDKAVAQNMDSVFKELLGKAAVRQGHGLASTASPSLGTRNLKPLACSSFSKNKVFSRGPGAPVSPSVSHPQGPDSTRKPV

Target ORF information:

RefSeq Version	XM_006229545.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229545.3

ORF Insert Sequence:

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGGG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAG TAAGGACCTG GTGCAGCAAC TGCAGGATGA GCTCCTGGAG 
GTGGTCTCAG AACTCCAGAC AACCAAGAAG ACGTACCATG TATACCACTT GGAGAGCATG 
AATGCTGAGA CTAAACTTCG GGAAGCTGAG AGGCAAGAGG AGAAGCGGTC AGGCAAGAGT 
GCCTTGCCCA CCACTACTGC TGCCTCTGCC ACCACCACCA TCACCATTGA GACAGAGCTC 
CACCGAAAGG GCTCTCTCAA GAAAGGAGGG CGGCTAGTGG AGAAGCGTCA GGCCAAGTTC 
TTGGAACATA AACTTAAATG CACCAAGGCC CGAAATGAGT ACCTGCTCAG CCTGGCCAGT 
GTCAATGCTG CTGTCAGCAA CTACTACTTA CACGATATCT TGGACCTCAT GGATTGCTGT 
GACACAGGTT TCCACTTGGC CCTGGAGCAG GCCCTCCGAA GCTACACAGC CGCTGAAAGC 
CGCACCCAAA CCTCTCACAT GCAGGGTTTA GGCAGTCTGG AAGAGGCTCT AGAGGCCTTG 
GATCCTCCAG GGGACAAAGC CAAAGTGCTT GAGGTTCATG CCATGGCCTT CTGTCCCCCT 
CTACGTTTTG ACTACCAGCC CCATGAAGGT GATGAGGTGG CTGAGATCCA GGTTGAGATG 
GAACTTCGGG ATGAGATTTT GCCCAGAGCC CAAAACATCC AAAGTCGCCT GGACCAAAAG 
ACCATTGAGA CAGAAGAGGT GAATAAGACA CTGAAGGCAA CACTTCAGGC TCTGCTAGAG 
GTGGTGGCAT CAGAGGATGG AGATATACTG GACTCTTTGC AGGCCAGCCC CTCCACTGAG 
TCCCTCAAGT CTACAAGCTC AGACCCAGGC ACCCGACAGG CAGGCCGCAG ACGGAACCAG 
CAGCAGGAGA CAGAAACCTT CTATATTACG AAGCTGCAGG AATATCTGAG TGGCCGGAGC 
ATCCTTGCCA AGCTGCAGGC CAAGCATGAA AAGCTCCAGG AAGCCATACA ACGAGGTAAC 
AAGGAAGAGC AAGAGACATC TTCTTGGACC CAATGCACAG AGAGAAAATT CCATAAGAGC 
CACCACCCCT ATCCTGGATC CCAGTGTACC CAGAGACTTT TTGGAGGTGA TCTAGAGAAG 
TTTATCCAGA GCACAGGCCA ACCTGTGCCC CTTGTGGTGA AGAGCTGTAT CCGCTTCATT 
AACCTCAATG GCCTGCAACA TGAAGGCATC TTCCGGGTAT CAGGTGCCCA GGCCCGGATA 
TTGGAGATCC GAGATGCCTT TGAAAGAGGG GAGGACCCTC TGGTGGAAGG CTGTACTGCC 
CATGACCTAG ACTCAGTGGC TGGGGTACTA AAGCTGTACT TCCGGAGCCT GGAGCCCCCA 
CTGTTCCCCT TGGACATGTT TAATGAGCTG CTGGCTTCAG CAGAACTGGA AGTTGTGGGT 
GAGCGGGTAG AGCTTGTGAG CCATCTACTA TCCAGACTAC CCAGACCTGT GCTAGTGGTC 
TTGCGATATC TCTTCACCTT CCTCAACCAC CTGGCCCAGT ACAGCGATGA GAACATGATG 
GACTCCTACA ACTTGGCTGT ATGTTTTGGG CCCACGCTGT TGCCTGTGCC TGCTGGGCAG 
GACCCTGTAG CCTTGCAGGG CCGAGTGAAC CAGCTGGTCC AGACCCTTAT CCTGCAACCA 
GCATGGGTTT TCCCACCCAC AGCCATGCTA CCAGGTCCCA TCTATGAGAA GTGCATGGCA 
CCACCTTCTG CCAGCTGCCT GGGGGATGGC CAGTTGGAGA GCCTTGCTGG GGAGCCAGAG 
CTGGAATTGA AAACTGGGAC CAAAGCTCAG GAAGATGACT CCGAGGGAGT TGTGGAGGCT 
GTGGCCTGCT TTGCTTACAC TGGCCGCACG GCCCAGGAGC TGACATTCCA GCAAGGGGAT 
ACGCTGAGAT TGTATGCTCG GGCATCAAGT GACTGGTGGC GAGGGGAGCA TGCTGGTGTG 
CGGGGGCTCA TTCCCCATAA GTACATCACA CTGTCTGAGG GGGATGAGAA GCAGATGACT 
GGTTCAGGAT TACAGGCCAT GGGGGAATCT CTGAGCCATC CAGAAAGCGT CCTGACCTTG 
GAGTTTACCA ACCGACTGGA ACTAGGCACC CCACCTGAGG CTTTGGGCCC CTCTGGTCAC 
AGACGACACT GTTTGGTCCC TACCTCCCCT GAGCAACATG TGGAAATGGA TAAGGCTGTG 
GCGCAGAACA TGGATTCTGT GTTTAAGGAG CTCTTGGGAA AGGCTGCTGT CCGCCAGGGC 
CATGGGCTAG CATCTACAGC CTCCCCCAGT CTAGGAACTC GAAACCTGAA GCCCCTGGCC 
TGCAGCAGCT TCAGCAAAAA CAAAGTCTTC TCCCGGGGAC CTGGGGCTCC AGTTTCCCCC 
TCAGTTTCCC ATCCCCAGGG TCCAGATTCA ACTCGCAAGC CAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap4 ( XM_006229546.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229608.1 Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa23005
Clone ID Related Accession (Same CDS sequence)	XM_006229546.3
Accession Version	XM_006229546.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rho GTPase-activating protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGGG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAC CAAGAAGACG TACCATGTAT ACCACTTGGA GAGCATGAAT 
GCTGAGACTA AACTTCGGGA AGCTGAGAGG CAAGAGGAGA AGCGGTCAGG CAAGAGTGCC 
TTGCCCACCA CTACTGCTGC CTCTGCCACC ACCACCATCA CCATTGAGAC AGAGCTCCAC 
CGAAAGGGCT CTCTCAAGAA AGGAGGGCGG CTAGTGGAGA AGCGTCAGGC CAAGTTCTTG 
GAACATAAAC TTAAATGCAC CAAGGCCCGA AATGAGTACC TGCTCAGCCT GGCCAGTGTC 
AATGCTGCTG TCAGCAACTA CTACTTACAC GATATCTTGG ACCTCATGGA TTGCTGTGAC 
ACAGGTTTCC ACTTGGCCCT GGAGCAGGCC CTCCGAAGCT ACACAGCCGC TGAAAGCCGC 
ACCCAAACCT CTCACATGCA GGGTTTAGGC AGTCTGGAAG AGGCTCTAGA GGCCTTGGAT 
CCTCCAGGGG ACAAAGCCAA AGTGCTTGAG GTTCATGCCA TGGCCTTCTG TCCCCCTCTA 
CGTTTTGACT ACCAGCCCCA TGAAGGTGAT GAGGTGGCTG AGATCCAGGT TGAGATGGAA 
CTTCGGGATG AGATTTTGCC CAGAGCCCAA AACATCCAAA GTCGCCTGGA CCAAAAGACC 
ATTGAGACAG AAGAGGTGAA TAAGACACTG AAGGCAACAC TTCAGGCTCT GCTAGAGGTG 
GTGGCATCAG AGGATGGAGA TATACTGGAC TCTTTGCAGG CCAGCCCCTC CACTGAGTCC 
CTCAAGTCTA CAAGCTCAGA CCCAGGCACC CGACAGGCAG GCCGCAGACG GAACCAGCAG 
CAGGAGACAG AAACCTTCTA TATTACGAAG CTGCAGGAAT ATCTGAGTGG CCGGAGCATC 
CTTGCCAAGC TGCAGGCCAA GCATGAAAAG CTCCAGGAAG CCATACAACG AGGTAACAAG 
GAAGAGCAAG AGACATCTTC TTGGACCCAA TGCACAGAGA GAAAATTCCA TAAGAGCCAC 
CACCCCTATC CTGGATCCCA GTGTACCCAG AGACTTTTTG GAGGTGATCT AGAGAAGTTT 
ATCCAGAGCA CAGGCCAACC TGTGCCCCTT GTGGTGAAGA GCTGTATCCG CTTCATTAAC 
CTCAATGGCC TGCAACATGA AGGCATCTTC CGGGTATCAG GTGCCCAGGC CCGGATATTG 
GAGATCCGAG ATGCCTTTGA AAGAGGGGAG GACCCTCTGG TGGAAGGCTG TACTGCCCAT 
GACCTAGACT CAGTGGCTGG GGTACTAAAG CTGTACTTCC GGAGCCTGGA GCCCCCACTG 
TTCCCCTTGG ACATGTTTAA TGAGCTGCTG GCTTCAGCAG AACTGGAAGT TGTGGGTGAG 
CGGGTAGAGC TTGTGAGCCA TCTACTATCC AGACTACCCA GACCTGTGCT AGTGGTCTTG 
CGATATCTCT TCACCTTCCT CAACCACCTG GCCCAGTACA GCGATGAGAA CATGATGGAC 
TCCTACAACT TGGCTGTATG TTTTGGGCCC ACGCTGTTGC CTGTGCCTGC TGGGCAGGAC 
CCTGTAGCCT TGCAGGGCCG AGTGAACCAG CTGGTCCAGA CCCTTATCCT GCAACCAGCA 
TGGGTTTTCC CACCCACAGC CATGCTACCA GGTCCCATCT ATGAGAAGTG CATGGCACCA 
CCTTCTGCCA GCTGCCTGGG GGATGGCCAG TTGGAGAGCC TTGCTGGGGA GCCAGAGCTG 
GAATTGAAAA CTGGGACCAA AGCTCAGGAA GATGACTCCG AGGGAGTTGT GGAGGCTGTG 
GCCTGCTTTG CTTACACTGG CCGCACGGCC CAGGAGCTGA CATTCCAGCA AGGGGATACG 
CTGAGATTGT ATGCTCGGGC ATCAAGTGAC TGGTGGCGAG GGGAGCATGC TGGTGTGCGG 
GGGCTCATTC CCCATAAGTA CATCACACTG TCTGAGGGGG ATGAGAAGCA GATGACTGGT 
TCAGGATTAC AGGCCATGGG GGAATCTCTG AGCCATCCAG AAAGCGTCCT GACCTTGGAG 
TTTACCAACC GACTGGAACT AGGCACCCCA CCTGAGGCTT TGGGCCCCTC TGGTCACAGA 
CGACACTGTT TGGTCCCTAC CTCCCCTGAG CAACATGTGG AAATGGATAA GGCTGTGGCG 
CAGAACATGG ATTCTGTGTT TAAGGAGCTC TTGGGAAAGG CTGCTGTCCG CCAGGGCCAT 
GGGCTAGCAT CTACAGCCTC CCCCAGTCTA GGAACTCGAA ACCTGAAGCC CCTGGCCTGC 
AGCAGCTTCA GCAAAAACAA AGTCTTCTCC CGGGGACCTG GGGCTCCAGT TTCCCCCTCA 
GTTTCCCATC CCCAGGGTCC AGATTCAACT CGCAAGCCAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229608.1
CDS	895..3699
Translation	MAAHGKLRRERGLQPEYEAQVKVMRGQLSEQLHCLELQGELRRDLLLELAEFMRRRAEVELEYSRGLDKLAERFTSRSGRLGGSSREQQSFRKEPTLLSPLHCWAMLLEHTRQQSRESATLSEVLAGPLAQRLSHIAEDVGRIVKKTKKTYHVYHLESMNAETKLREAERQEEKRSGKSALPTTTAASATTTITIETELHRKGSLKKGGRLVEKRQAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLHDILDLMDCCDTGFHLALEQALRSYTAAESRTQTSHMQGLGSLEEALEALDPPGDKAKVLEVHAMAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDQKTIETEEVNKTLKATLQALLEVVASEDGDILDSLQASPSTESLKSTSSDPGTRQAGRRRNQQQETETFYITKLQEYLSGRSILAKLQAKHEKLQEAIQRGNKEEQETSSWTQCTERKFHKSHHPYPGSQCTQRLFGGDLEKFIQSTGQPVPLVVKSCIRFINLNGLQHEGIFRVSGAQARILEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLEPPLFPLDMFNELLASAELEVVGERVELVSHLLSRLPRPVLVVLRYLFTFLNHLAQYSDENMMDSYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLILQPAWVFPPTAMLPGPIYEKCMAPPSASCLGDGQLESLAGEPELELKTGTKAQEDDSEGVVEAVACFAYTGRTAQELTFQQGDTLRLYARASSDWWRGEHAGVRGLIPHKYITLSEGDEKQMTGSGLQAMGESLSHPESVLTLEFTNRLELGTPPEALGPSGHRRHCLVPTSPEQHVEMDKAVAQNMDSVFKELLGKAAVRQGHGLASTASPSLGTRNLKPLACSSFSKNKVFSRGPGAPVSPSVSHPQGPDSTRKPV

Target ORF information:

RefSeq Version	XM_006229546.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229546.3

ORF Insert Sequence:

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGGG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAC CAAGAAGACG TACCATGTAT ACCACTTGGA GAGCATGAAT 
GCTGAGACTA AACTTCGGGA AGCTGAGAGG CAAGAGGAGA AGCGGTCAGG CAAGAGTGCC 
TTGCCCACCA CTACTGCTGC CTCTGCCACC ACCACCATCA CCATTGAGAC AGAGCTCCAC 
CGAAAGGGCT CTCTCAAGAA AGGAGGGCGG CTAGTGGAGA AGCGTCAGGC CAAGTTCTTG 
GAACATAAAC TTAAATGCAC CAAGGCCCGA AATGAGTACC TGCTCAGCCT GGCCAGTGTC 
AATGCTGCTG TCAGCAACTA CTACTTACAC GATATCTTGG ACCTCATGGA TTGCTGTGAC 
ACAGGTTTCC ACTTGGCCCT GGAGCAGGCC CTCCGAAGCT ACACAGCCGC TGAAAGCCGC 
ACCCAAACCT CTCACATGCA GGGTTTAGGC AGTCTGGAAG AGGCTCTAGA GGCCTTGGAT 
CCTCCAGGGG ACAAAGCCAA AGTGCTTGAG GTTCATGCCA TGGCCTTCTG TCCCCCTCTA 
CGTTTTGACT ACCAGCCCCA TGAAGGTGAT GAGGTGGCTG AGATCCAGGT TGAGATGGAA 
CTTCGGGATG AGATTTTGCC CAGAGCCCAA AACATCCAAA GTCGCCTGGA CCAAAAGACC 
ATTGAGACAG AAGAGGTGAA TAAGACACTG AAGGCAACAC TTCAGGCTCT GCTAGAGGTG 
GTGGCATCAG AGGATGGAGA TATACTGGAC TCTTTGCAGG CCAGCCCCTC CACTGAGTCC 
CTCAAGTCTA CAAGCTCAGA CCCAGGCACC CGACAGGCAG GCCGCAGACG GAACCAGCAG 
CAGGAGACAG AAACCTTCTA TATTACGAAG CTGCAGGAAT ATCTGAGTGG CCGGAGCATC 
CTTGCCAAGC TGCAGGCCAA GCATGAAAAG CTCCAGGAAG CCATACAACG AGGTAACAAG 
GAAGAGCAAG AGACATCTTC TTGGACCCAA TGCACAGAGA GAAAATTCCA TAAGAGCCAC 
CACCCCTATC CTGGATCCCA GTGTACCCAG AGACTTTTTG GAGGTGATCT AGAGAAGTTT 
ATCCAGAGCA CAGGCCAACC TGTGCCCCTT GTGGTGAAGA GCTGTATCCG CTTCATTAAC 
CTCAATGGCC TGCAACATGA AGGCATCTTC CGGGTATCAG GTGCCCAGGC CCGGATATTG 
GAGATCCGAG ATGCCTTTGA AAGAGGGGAG GACCCTCTGG TGGAAGGCTG TACTGCCCAT 
GACCTAGACT CAGTGGCTGG GGTACTAAAG CTGTACTTCC GGAGCCTGGA GCCCCCACTG 
TTCCCCTTGG ACATGTTTAA TGAGCTGCTG GCTTCAGCAG AACTGGAAGT TGTGGGTGAG 
CGGGTAGAGC TTGTGAGCCA TCTACTATCC AGACTACCCA GACCTGTGCT AGTGGTCTTG 
CGATATCTCT TCACCTTCCT CAACCACCTG GCCCAGTACA GCGATGAGAA CATGATGGAC 
TCCTACAACT TGGCTGTATG TTTTGGGCCC ACGCTGTTGC CTGTGCCTGC TGGGCAGGAC 
CCTGTAGCCT TGCAGGGCCG AGTGAACCAG CTGGTCCAGA CCCTTATCCT GCAACCAGCA 
TGGGTTTTCC CACCCACAGC CATGCTACCA GGTCCCATCT ATGAGAAGTG CATGGCACCA 
CCTTCTGCCA GCTGCCTGGG GGATGGCCAG TTGGAGAGCC TTGCTGGGGA GCCAGAGCTG 
GAATTGAAAA CTGGGACCAA AGCTCAGGAA GATGACTCCG AGGGAGTTGT GGAGGCTGTG 
GCCTGCTTTG CTTACACTGG CCGCACGGCC CAGGAGCTGA CATTCCAGCA AGGGGATACG 
CTGAGATTGT ATGCTCGGGC ATCAAGTGAC TGGTGGCGAG GGGAGCATGC TGGTGTGCGG 
GGGCTCATTC CCCATAAGTA CATCACACTG TCTGAGGGGG ATGAGAAGCA GATGACTGGT 
TCAGGATTAC AGGCCATGGG GGAATCTCTG AGCCATCCAG AAAGCGTCCT GACCTTGGAG 
TTTACCAACC GACTGGAACT AGGCACCCCA CCTGAGGCTT TGGGCCCCTC TGGTCACAGA 
CGACACTGTT TGGTCCCTAC CTCCCCTGAG CAACATGTGG AAATGGATAA GGCTGTGGCG 
CAGAACATGG ATTCTGTGTT TAAGGAGCTC TTGGGAAAGG CTGCTGTCCG CCAGGGCCAT 
GGGCTAGCAT CTACAGCCTC CCCCAGTCTA GGAACTCGAA ACCTGAAGCC CCTGGCCTGC 
AGCAGCTTCA GCAAAAACAA AGTCTTCTCC CGGGGACCTG GGGCTCCAGT TTCCCCCTCA 
GTTTCCCATC CCCAGGGTCC AGATTCAACT CGCAAGCCAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap4 ( XM_006229547.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229609.1 Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa23006
Clone ID Related Accession (Same CDS sequence)	XM_006229547.3
Accession Version	XM_006229547.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rho GTPase-activating protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGGG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAG TAAGGACCTG GTGCAGCAAC TGCAGGATGA GCTCCTGGAG 
GTGGTCTCAG AACTCCAGAC AACCAAGAAG ACGTACCATG TATACCACTT GGAGAGCATG 
AATGCTGAGA CTAAACTTCG GGAAGCTGAG AGGCAAGAGG AGAAGCGGTC AGGCAAGAGT 
GCCTTGCCCA CCACTACTGC TGCCTCTGCC ACCACCACCA TCACCATTGA GACAGAGCTC 
CACCGAAAGG GCTCTCTCAA GAAAGGAGGG CGGCTAGTGG AGAAGCGTCA GGCCAAGTTC 
TTGGAACATA AACTTAAATG CACCAAGGCC CGAAATGAGT ACCTGCTCAG CCTGGCCAGT 
GTCAATGCTG CTGTCAGCAA CTACTACTTA CACGATATCT TGGACCTCAT GGATTGCTGT 
GACACAGGTT TCCACTTGGC CCTGGAGCAG GCCCTCCGAA GCTACACAGC CGCTGAAAGC 
CGCACCCAAA CCTCTCACAT GCAGGGTTTA GGCAGTCTGG AAGAGGCTCT AGAGGCCTTG 
GATCCTCCAG GGGACAAAGC CAAAGTGCTT GAGGTTCATG CCATGGCCTT CTGTCCCCCT 
CTACGTTTTG ACTACCAGCC CCATGAAGGT GATGAGGTGA ATAAGACACT GAAGGCAACA 
CTTCAGGCTC TGCTAGAGGT GGTGGCATCA GAGGATGGAG ATATACTGGA CTCTTTGCAG 
GCCAGCCCCT CCACTGAGTC CCTCAAGTCT ACAAGCTCAG ACCCAGGCAC CCGACAGGCA 
GGCCGCAGAC GGAACCAGCA GCAGGAGACA GAAACCTTCT ATATTACGAA GCTGCAGGAA 
TATCTGAGTG GCCGGAGCAT CCTTGCCAAG CTGCAGGCCA AGCATGAAAA GCTCCAGGAA 
GCCATACAAC GAGGTAACAA GGAAGAGCAA GAGACATCTT CTTGGACCCA ATGCACAGAG 
AGAAAATTCC ATAAGAGCCA CCACCCCTAT CCTGGATCCC AGTGTACCCA GAGACTTTTT 
GGAGGTGATC TAGAGAAGTT TATCCAGAGC ACAGGCCAAC CTGTGCCCCT TGTGGTGAAG 
AGCTGTATCC GCTTCATTAA CCTCAATGGC CTGCAACATG AAGGCATCTT CCGGGTATCA 
GGTGCCCAGG CCCGGATATT GGAGATCCGA GATGCCTTTG AAAGAGGGGA GGACCCTCTG 
GTGGAAGGCT GTACTGCCCA TGACCTAGAC TCAGTGGCTG GGGTACTAAA GCTGTACTTC 
CGGAGCCTGG AGCCCCCACT GTTCCCCTTG GACATGTTTA ATGAGCTGCT GGCTTCAGCA 
GAACTGGAAG TTGTGGGTGA GCGGGTAGAG CTTGTGAGCC ATCTACTATC CAGACTACCC 
AGACCTGTGC TAGTGGTCTT GCGATATCTC TTCACCTTCC TCAACCACCT GGCCCAGTAC 
AGCGATGAGA ACATGATGGA CTCCTACAAC TTGGCTGTAT GTTTTGGGCC CACGCTGTTG 
CCTGTGCCTG CTGGGCAGGA CCCTGTAGCC TTGCAGGGCC GAGTGAACCA GCTGGTCCAG 
ACCCTTATCC TGCAACCAGC ATGGGTTTTC CCACCCACAG CCATGCTACC AGGTCCCATC 
TATGAGAAGT GCATGGCACC ACCTTCTGCC AGCTGCCTGG GGGATGGCCA GTTGGAGAGC 
CTTGCTGGGG AGCCAGAGCT GGAATTGAAA ACTGGGACCA AAGCTCAGGA AGATGACTCC 
GAGGGAGTTG TGGAGGCTGT GGCCTGCTTT GCTTACACTG GCCGCACGGC CCAGGAGCTG 
ACATTCCAGC AAGGGGATAC GCTGAGATTG TATGCTCGGG CATCAAGTGA CTGGTGGCGA 
GGGGAGCATG CTGGTGTGCG GGGGCTCATT CCCCATAAGT ACATCACACT GTCTGAGGGG 
GATGAGAAGC AGATGACTGG TTCAGGATTA CAGGCCATGG GGGAATCTCT GAGCCATCCA 
GAAAGCGTCC TGACCTTGGA GTTTACCAAC CGACTGGAAC TAGGCACCCC ACCTGAGGCT 
TTGGGCCCCT CTGGTCACAG ACGACACTGT TTGGTCCCTA CCTCCCCTGA GCAACATGTG 
GAAATGGATA AGGCTGTGGC GCAGAACATG GATTCTGTGT TTAAGGAGCT CTTGGGAAAG 
GCTGCTGTCC GCCAGGGCCA TGGGCTAGCA TCTACAGCCT CCCCCAGTCT AGGAACTCGA 
AACCTGAAGC CCCTGGCCTG CAGCAGCTTC AGCAAAAACA AAGTCTTCTC CCGGGGACCT 
GGGGCTCCAG TTTCCCCCTC AGTTTCCCAT CCCCAGGGTC CAGATTCAAC TCGCAAGCCA 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229609.1
CDS	895..3660
Translation	MAAHGKLRRERGLQPEYEAQVKVMRGQLSEQLHCLELQGELRRDLLLELAEFMRRRAEVELEYSRGLDKLAERFTSRSGRLGGSSREQQSFRKEPTLLSPLHCWAMLLEHTRQQSRESATLSEVLAGPLAQRLSHIAEDVGRIVKKSKDLVQQLQDELLEVVSELQTTKKTYHVYHLESMNAETKLREAERQEEKRSGKSALPTTTAASATTTITIETELHRKGSLKKGGRLVEKRQAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLHDILDLMDCCDTGFHLALEQALRSYTAAESRTQTSHMQGLGSLEEALEALDPPGDKAKVLEVHAMAFCPPLRFDYQPHEGDEVNKTLKATLQALLEVVASEDGDILDSLQASPSTESLKSTSSDPGTRQAGRRRNQQQETETFYITKLQEYLSGRSILAKLQAKHEKLQEAIQRGNKEEQETSSWTQCTERKFHKSHHPYPGSQCTQRLFGGDLEKFIQSTGQPVPLVVKSCIRFINLNGLQHEGIFRVSGAQARILEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLEPPLFPLDMFNELLASAELEVVGERVELVSHLLSRLPRPVLVVLRYLFTFLNHLAQYSDENMMDSYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLILQPAWVFPPTAMLPGPIYEKCMAPPSASCLGDGQLESLAGEPELELKTGTKAQEDDSEGVVEAVACFAYTGRTAQELTFQQGDTLRLYARASSDWWRGEHAGVRGLIPHKYITLSEGDEKQMTGSGLQAMGESLSHPESVLTLEFTNRLELGTPPEALGPSGHRRHCLVPTSPEQHVEMDKAVAQNMDSVFKELLGKAAVRQGHGLASTASPSLGTRNLKPLACSSFSKNKVFSRGPGAPVSPSVSHPQGPDSTRKPV

Target ORF information:

RefSeq Version	XM_006229547.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229547.3

ORF Insert Sequence:

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGGG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAG TAAGGACCTG GTGCAGCAAC TGCAGGATGA GCTCCTGGAG 
GTGGTCTCAG AACTCCAGAC AACCAAGAAG ACGTACCATG TATACCACTT GGAGAGCATG 
AATGCTGAGA CTAAACTTCG GGAAGCTGAG AGGCAAGAGG AGAAGCGGTC AGGCAAGAGT 
GCCTTGCCCA CCACTACTGC TGCCTCTGCC ACCACCACCA TCACCATTGA GACAGAGCTC 
CACCGAAAGG GCTCTCTCAA GAAAGGAGGG CGGCTAGTGG AGAAGCGTCA GGCCAAGTTC 
TTGGAACATA AACTTAAATG CACCAAGGCC CGAAATGAGT ACCTGCTCAG CCTGGCCAGT 
GTCAATGCTG CTGTCAGCAA CTACTACTTA CACGATATCT TGGACCTCAT GGATTGCTGT 
GACACAGGTT TCCACTTGGC CCTGGAGCAG GCCCTCCGAA GCTACACAGC CGCTGAAAGC 
CGCACCCAAA CCTCTCACAT GCAGGGTTTA GGCAGTCTGG AAGAGGCTCT AGAGGCCTTG 
GATCCTCCAG GGGACAAAGC CAAAGTGCTT GAGGTTCATG CCATGGCCTT CTGTCCCCCT 
CTACGTTTTG ACTACCAGCC CCATGAAGGT GATGAGGTGA ATAAGACACT GAAGGCAACA 
CTTCAGGCTC TGCTAGAGGT GGTGGCATCA GAGGATGGAG ATATACTGGA CTCTTTGCAG 
GCCAGCCCCT CCACTGAGTC CCTCAAGTCT ACAAGCTCAG ACCCAGGCAC CCGACAGGCA 
GGCCGCAGAC GGAACCAGCA GCAGGAGACA GAAACCTTCT ATATTACGAA GCTGCAGGAA 
TATCTGAGTG GCCGGAGCAT CCTTGCCAAG CTGCAGGCCA AGCATGAAAA GCTCCAGGAA 
GCCATACAAC GAGGTAACAA GGAAGAGCAA GAGACATCTT CTTGGACCCA ATGCACAGAG 
AGAAAATTCC ATAAGAGCCA CCACCCCTAT CCTGGATCCC AGTGTACCCA GAGACTTTTT 
GGAGGTGATC TAGAGAAGTT TATCCAGAGC ACAGGCCAAC CTGTGCCCCT TGTGGTGAAG 
AGCTGTATCC GCTTCATTAA CCTCAATGGC CTGCAACATG AAGGCATCTT CCGGGTATCA 
GGTGCCCAGG CCCGGATATT GGAGATCCGA GATGCCTTTG AAAGAGGGGA GGACCCTCTG 
GTGGAAGGCT GTACTGCCCA TGACCTAGAC TCAGTGGCTG GGGTACTAAA GCTGTACTTC 
CGGAGCCTGG AGCCCCCACT GTTCCCCTTG GACATGTTTA ATGAGCTGCT GGCTTCAGCA 
GAACTGGAAG TTGTGGGTGA GCGGGTAGAG CTTGTGAGCC ATCTACTATC CAGACTACCC 
AGACCTGTGC TAGTGGTCTT GCGATATCTC TTCACCTTCC TCAACCACCT GGCCCAGTAC 
AGCGATGAGA ACATGATGGA CTCCTACAAC TTGGCTGTAT GTTTTGGGCC CACGCTGTTG 
CCTGTGCCTG CTGGGCAGGA CCCTGTAGCC TTGCAGGGCC GAGTGAACCA GCTGGTCCAG 
ACCCTTATCC TGCAACCAGC ATGGGTTTTC CCACCCACAG CCATGCTACC AGGTCCCATC 
TATGAGAAGT GCATGGCACC ACCTTCTGCC AGCTGCCTGG GGGATGGCCA GTTGGAGAGC 
CTTGCTGGGG AGCCAGAGCT GGAATTGAAA ACTGGGACCA AAGCTCAGGA AGATGACTCC 
GAGGGAGTTG TGGAGGCTGT GGCCTGCTTT GCTTACACTG GCCGCACGGC CCAGGAGCTG 
ACATTCCAGC AAGGGGATAC GCTGAGATTG TATGCTCGGG CATCAAGTGA CTGGTGGCGA 
GGGGAGCATG CTGGTGTGCG GGGGCTCATT CCCCATAAGT ACATCACACT GTCTGAGGGG 
GATGAGAAGC AGATGACTGG TTCAGGATTA CAGGCCATGG GGGAATCTCT GAGCCATCCA 
GAAAGCGTCC TGACCTTGGA GTTTACCAAC CGACTGGAAC TAGGCACCCC ACCTGAGGCT 
TTGGGCCCCT CTGGTCACAG ACGACACTGT TTGGTCCCTA CCTCCCCTGA GCAACATGTG 
GAAATGGATA AGGCTGTGGC GCAGAACATG GATTCTGTGT TTAAGGAGCT CTTGGGAAAG 
GCTGCTGTCC GCCAGGGCCA TGGGCTAGCA TCTACAGCCT CCCCCAGTCT AGGAACTCGA 
AACCTGAAGC CCCTGGCCTG CAGCAGCTTC AGCAAAAACA AAGTCTTCTC CCGGGGACCT 
GGGGCTCCAG TTTCCCCCTC AGTTTCCCAT CCCCAGGGTC CAGATTCAAC TCGCAAGCCA 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap4 ( XM_006229548.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229610.1 Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X4, mRNA.

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CloneID	ORa23007
Clone ID Related Accession (Same CDS sequence)	XM_006229548.3
Accession Version	XM_006229548.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rho GTPase-activating protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229548.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGGG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAG TAAGGACCTG GTGCAGCAAC TGCAGGATGA GCTCCTGGAG 
GTGGTCTCAG AACTCCAGAC AACCAAGAAG ACGTACCATG TATACCACTT GGAGAGCATG 
AATGCTGAGA CTAAACTTCG GGAAGCTGAG AGGCAAGAGG AGAAGCGGTC AGGCAAGAGT 
GCCTTGCCCA CCACTACTGC TGCCTCTGCC ACCACCACCA TCACCATTGA GACAGAGCTC 
CACCGAAAGG GCTCTCTCAA GAAAGGAGGG CGGCTAGTGG AGAAGCGTCA GGCCAAGTTC 
TTGGAACATA AACTTAAATG CACCAAGGCC CGAAATGAGT ACCTGCTCAG CCTGGCCAGT 
GTCAATGCTG CTGTCAGCAA CTACTACTTA CACGATATCT TGGACCTCAT GGATTGCTGT 
GACACAGGTT TCCACTTGGC CCTGGAGCAG GCCCTCCGAA GCTACACAGC CGCTGAAAGC 
CGCACCCAAA CCTCTCACAT GCAGGGTTTA GGCAGTCTGG AAGAGGCTCT AGAGGCCTTG 
GATCCTCCAG GGGACAAAGC CAAAGTGCTT GAGGTTCATG CCATGGCCTT CTGTCCCCCT 
CTACGTTTTG ACTACCAGCC CCATGAAGGT GATGAGGTGG CTGAGATCCA GGTTGAGATG 
GAACTTCGGG ATGAGATTTT GCCCAGAGCC CAAAACATCC AAAGTCGCCT GGACCAAAAG 
ACCATTGAGA CAGAAGAGGT GAATAAGACA CTGAAGGCAA CACTTCAGGC TCTGCTAGAG 
GTGGTGGCAT CAGAGGATGG AGATATACTG GACTCTTTGC AGGCCAGCCC CTCCACTGAG 
TCCCTCAAGT CTACAAGCTC AGACCCAGGC ACCCGACAGG CAGGCCGCAG ACGGAACCAG 
CAGCAGGAGA CAGAAACCTT CTATATTACG AAGCTGCAGG AATATCTGAG TGGCCGGAGC 
ATCCTTGCCA AGCTGCAGGC CAAGCATGAA AAGCTCCAGG AAGCCATACA ACGAGGTAAC 
AAGGAAGAGC AAGAGACATC TTCTTGGACC CAATGCACAG AGAGAAAATT CCATAAGAGC 
CACCACCCCT ATCCTGGATC CCAGTGTACC CAGAGACTTT TTGGAGGTGA TCTAGAGAAG 
TTTATCCAGA GCACAGGCCA ACCTGTGCCC CTTGTGGTGA AGAGCTGTAT CCGCTTCATT 
AACCTCAATG GCCTGCAACA TGAAGGCATC TTCCGGGTAT CAGGTGCCCA GGCCCGGATA 
TTGGAGATCC GAGATGCCTT TGAAAGAGGG GAGGACCCTC TGGTGGAAGG CTGTACTGCC 
CATGACCTAG ACTCAGTGGC TGGGGTACTA AAGCTGTACT TCCGGAGCCT GGAGCCCCCA 
CTGTTCCCCT TGGACATGTT TAATGAGCTG CTGGCTTCAG CAGAACTGGA AGTTGTGGGT 
GAGCGGGTAG AGCTTGTGAG CCATCTACTA TCCAGACTAC CCAGACCTGT GCTAGTGGTC 
TTGCGATATC TCTTCACCTT CCTCAACCAG GATGGCCAGT TGGAGAGCCT TGCTGGGGAG 
CCAGAGCTGG AATTGAAAAC TGGGACCAAA GCTCAGGAAG ATGACTCCGA GGGAGTTGTG 
GAGGCTGTGG CCTGCTTTGC TTACACTGGC CGCACGGCCC AGGAGCTGAC ATTCCAGCAA 
GGGGATACGC TGAGATTGTA TGCTCGGGCA TCAAGTGACT GGTGGCGAGG GGAGCATGCT 
GGTGTGCGGG GGCTCATTCC CCATAAGTAC ATCACACTGT CTGAGGGGGA TGAGAAGCAG 
ATGACTGGTT CAGGATTACA GGCCATGGGG GAATCTCTGA GCCATCCAGA AAGCGTCCTG 
ACCTTGGAGT TTACCAACCG ACTGGAACTA GGCACCCCAC CTGAGGCTTT GGGCCCCTCT 
GGTCACAGAC GACACTGTTT GGTCCCTACC TCCCCTGAGC AACATGTGGA AATGGATAAG 
GCTGTGGCGC AGAACATGGA TTCTGTGTTT AAGGAGCTCT TGGGAAAGGC TGCTGTCCGC 
CAGGGCCATG GGCTAGCATC TACAGCCTCC CCCAGTCTAG GAACTCGAAA CCTGAAGCCC 
CTGGCCTGCA GCAGCTTCAG CAAAAACAAA GTCTTCTCCC GGGGACCTGG GGCTCCAGTT 
TCCCCCTCAG TTTCCCATCC CCAGGGTCCA GATTCAACTC GCAAGCCAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229610.1
CDS	895..3528
Translation	MAAHGKLRRERGLQPEYEAQVKVMRGQLSEQLHCLELQGELRRDLLLELAEFMRRRAEVELEYSRGLDKLAERFTSRSGRLGGSSREQQSFRKEPTLLSPLHCWAMLLEHTRQQSRESATLSEVLAGPLAQRLSHIAEDVGRIVKKSKDLVQQLQDELLEVVSELQTTKKTYHVYHLESMNAETKLREAERQEEKRSGKSALPTTTAASATTTITIETELHRKGSLKKGGRLVEKRQAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLHDILDLMDCCDTGFHLALEQALRSYTAAESRTQTSHMQGLGSLEEALEALDPPGDKAKVLEVHAMAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDQKTIETEEVNKTLKATLQALLEVVASEDGDILDSLQASPSTESLKSTSSDPGTRQAGRRRNQQQETETFYITKLQEYLSGRSILAKLQAKHEKLQEAIQRGNKEEQETSSWTQCTERKFHKSHHPYPGSQCTQRLFGGDLEKFIQSTGQPVPLVVKSCIRFINLNGLQHEGIFRVSGAQARILEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLEPPLFPLDMFNELLASAELEVVGERVELVSHLLSRLPRPVLVVLRYLFTFLNQDGQLESLAGEPELELKTGTKAQEDDSEGVVEAVACFAYTGRTAQELTFQQGDTLRLYARASSDWWRGEHAGVRGLIPHKYITLSEGDEKQMTGSGLQAMGESLSHPESVLTLEFTNRLELGTPPEALGPSGHRRHCLVPTSPEQHVEMDKAVAQNMDSVFKELLGKAAVRQGHGLASTASPSLGTRNLKPLACSSFSKNKVFSRGPGAPVSPSVSHPQGPDSTRKPV

Target ORF information:

RefSeq Version	XM_006229548.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho GTPase activating protein 4 (Arhgap4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229548.3

ORF Insert Sequence:

ATGGCGGCGC ACGGGAAGTT GCGGCGGGAG CGGGGACTGC AACCCGAGTA TGAGGCACAG 
GTCAAAGTGA TGCGCGGGCA GTTGAGTGAG CAGCTTCATT GCCTGGAGCT TCAGGGAGAG 
CTGCGGCGGG ATTTGCTGCT GGAGCTAGCT GAGTTCATGC GGCGTCGAGC AGAGGTGGAG 
CTGGAGTATT CTCGGGGCCT GGACAAGTTG GCTGAACGCT TTACTAGCCG CAGTGGACGC 
CTTGGAGGCA GCAGCCGGGA GCAGCAAAGT TTCCGGAAGG AGCCAACTCT CCTGTCACCC 
TTGCACTGCT GGGCTATGCT GCTGGAACAT ACTCGGCAGC AGAGTCGGGA AAGTGCTACC 
CTCAGTGAGG TGCTGGCTGG ACCTCTGGCA CAGCGCCTGA GTCACATTGC CGAGGATGTA 
GGACGCATTG TCAAAAAGAG TAAGGACCTG GTGCAGCAAC TGCAGGATGA GCTCCTGGAG 
GTGGTCTCAG AACTCCAGAC AACCAAGAAG ACGTACCATG TATACCACTT GGAGAGCATG 
AATGCTGAGA CTAAACTTCG GGAAGCTGAG AGGCAAGAGG AGAAGCGGTC AGGCAAGAGT 
GCCTTGCCCA CCACTACTGC TGCCTCTGCC ACCACCACCA TCACCATTGA GACAGAGCTC 
CACCGAAAGG GCTCTCTCAA GAAAGGAGGG CGGCTAGTGG AGAAGCGTCA GGCCAAGTTC 
TTGGAACATA AACTTAAATG CACCAAGGCC CGAAATGAGT ACCTGCTCAG CCTGGCCAGT 
GTCAATGCTG CTGTCAGCAA CTACTACTTA CACGATATCT TGGACCTCAT GGATTGCTGT 
GACACAGGTT TCCACTTGGC CCTGGAGCAG GCCCTCCGAA GCTACACAGC CGCTGAAAGC 
CGCACCCAAA CCTCTCACAT GCAGGGTTTA GGCAGTCTGG AAGAGGCTCT AGAGGCCTTG 
GATCCTCCAG GGGACAAAGC CAAAGTGCTT GAGGTTCATG CCATGGCCTT CTGTCCCCCT 
CTACGTTTTG ACTACCAGCC CCATGAAGGT GATGAGGTGG CTGAGATCCA GGTTGAGATG 
GAACTTCGGG ATGAGATTTT GCCCAGAGCC CAAAACATCC AAAGTCGCCT GGACCAAAAG 
ACCATTGAGA CAGAAGAGGT GAATAAGACA CTGAAGGCAA CACTTCAGGC TCTGCTAGAG 
GTGGTGGCAT CAGAGGATGG AGATATACTG GACTCTTTGC AGGCCAGCCC CTCCACTGAG 
TCCCTCAAGT CTACAAGCTC AGACCCAGGC ACCCGACAGG CAGGCCGCAG ACGGAACCAG 
CAGCAGGAGA CAGAAACCTT CTATATTACG AAGCTGCAGG AATATCTGAG TGGCCGGAGC 
ATCCTTGCCA AGCTGCAGGC CAAGCATGAA AAGCTCCAGG AAGCCATACA ACGAGGTAAC 
AAGGAAGAGC AAGAGACATC TTCTTGGACC CAATGCACAG AGAGAAAATT CCATAAGAGC 
CACCACCCCT ATCCTGGATC CCAGTGTACC CAGAGACTTT TTGGAGGTGA TCTAGAGAAG 
TTTATCCAGA GCACAGGCCA ACCTGTGCCC CTTGTGGTGA AGAGCTGTAT CCGCTTCATT 
AACCTCAATG GCCTGCAACA TGAAGGCATC TTCCGGGTAT CAGGTGCCCA GGCCCGGATA 
TTGGAGATCC GAGATGCCTT TGAAAGAGGG GAGGACCCTC TGGTGGAAGG CTGTACTGCC 
CATGACCTAG ACTCAGTGGC TGGGGTACTA AAGCTGTACT TCCGGAGCCT GGAGCCCCCA 
CTGTTCCCCT TGGACATGTT TAATGAGCTG CTGGCTTCAG CAGAACTGGA AGTTGTGGGT 
GAGCGGGTAG AGCTTGTGAG CCATCTACTA TCCAGACTAC CCAGACCTGT GCTAGTGGTC 
TTGCGATATC TCTTCACCTT CCTCAACCAG GATGGCCAGT TGGAGAGCCT TGCTGGGGAG 
CCAGAGCTGG AATTGAAAAC TGGGACCAAA GCTCAGGAAG ATGACTCCGA GGGAGTTGTG 
GAGGCTGTGG CCTGCTTTGC TTACACTGGC CGCACGGCCC AGGAGCTGAC ATTCCAGCAA 
GGGGATACGC TGAGATTGTA TGCTCGGGCA TCAAGTGACT GGTGGCGAGG GGAGCATGCT 
GGTGTGCGGG GGCTCATTCC CCATAAGTAC ATCACACTGT CTGAGGGGGA TGAGAAGCAG 
ATGACTGGTT CAGGATTACA GGCCATGGGG GAATCTCTGA GCCATCCAGA AAGCGTCCTG 
ACCTTGGAGT TTACCAACCG ACTGGAACTA GGCACCCCAC CTGAGGCTTT GGGCCCCTCT 
GGTCACAGAC GACACTGTTT GGTCCCTACC TCCCCTGAGC AACATGTGGA AATGGATAAG 
GCTGTGGCGC AGAACATGGA TTCTGTGTTT AAGGAGCTCT TGGGAAAGGC TGCTGTCCGC 
CAGGGCCATG GGCTAGCATC TACAGCCTCC CCCAGTCTAG GAACTCGAAA CCTGAAGCCC 
CTGGCCTGCA GCAGCTTCAG CAAAAACAAA GTCTTCTCCC GGGGACCTGG GGCTCCAGTT 
TCCCCCTCAG TTTCCCATCC CCAGGGTCCA GATTCAACTC GCAAGCCAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Neuronal Migration Factor srGAP2 Achieves Specificity in Ligand Binding through a Two-Component Molecular Mechanism.
Structure (London, England : 1993)23(11)1989-2000(2015 Nov)
Guez-Haddad J,Sporny M,Sasson Y,Gevorkyan-Airapetov L,Lahav-Mankovski N,Margulies D,Radzimanowski J,Opatowsky Y

ARHGAP4 is a novel RhoGAP that mediates inhibition of cell motility and axon outgrowth.
Molecular and cellular neurosciences36(3)332-42(2007 Nov)
Vogt DL,Gray CD,Young WS 3rd,Orellana SA,Malouf AT

Cloning of rat ARHGAP4/C1, a RhoGAP family member expressed in the nervous system that colocalizes with the Golgi complex and microtubules.
Brain research. Molecular brain research107(1)65-79(2002 Oct)
Foletta VC,Brown FD,Young WS 3rd

RefSeq and LocusLink: NCBI gene-centered resources.
Nucleic acids research29(1)137-40(2001 Jan)
Pruitt KD,Maglott DR

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Arhgap4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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