arhgap4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgap4b
Entrez Gene ID	561231
Full Name	Rho GTPase activating protein 4b
Synonyms	si:dkey-95f11.4
General protein information	Preferred Names Rho GTPase activating protein 4b Names SLIT-ROBO Rho GTPase-activating protein 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296754.2	XP_009295029.1	SLIT-ROBO Rho GTPase-activating protein 3 isoform X2
XM_009296754.1	XP_009295029.1	rho GTPase-activating protein 4 isoform X2
XM_684637.7	XP_689729.3	SLIT-ROBO Rho GTPase-activating protein 3 isoform X1
XM_005162108.2	XP_005162165.1	SLIT-ROBO Rho GTPase-activating protein 3 isoform X3
XM_684637.6	XP_689729.3	rho GTPase-activating protein 4 isoform X1
XM_009296756.2	XP_009295031.1	rho GTPase-activating protein 4 isoform X5
XM_009296755.1	XP_009295030.1	SLIT-ROBO Rho GTPase-activating protein 3 isoform X4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARHGAP4	XP_549365.3
	ARHGAP4	XP_002806496.1
Danio rerio (zebrafish)	arhgap4b	XP_689729.3
Danio rerio (zebrafish)	arhgap4a	NP_956738.1
Homo sapiens (human)	ARHGAP4	NP_001158213.1
Pan troglodytes (chimpanzee)	ARHGAP4	XP_003317822.1
Mus musculus (house mouse)	Arhgap4	NP_619536.2
Xenopus tropicalis (tropical clawed frog)	arhgap4	NP_001123805.1
Bos taurus (cattle)	ARHGAP4	NP_001096010.1
Rattus norvegicus (Norway rat)	Arhgap4	NP_653341.2

Gene Ontology

The following arhgap4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgap4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa43468	XM_009296755.1 Latest version!	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa43469	XM_009296756.2 Latest version!	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa27626	XM_684637.7 Latest version!	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27627	XM_005162108.2 Latest version!	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa43467	XM_009296754.2 Latest version!	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa43467	XM_009296754.1	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27626	XM_684637.6	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgap4b ( XM_009296755.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295030.1 Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa43468
Clone ID Related Accession (Same CDS sequence)	XM_009296755.1
Accession Version	XM_009296755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTACT TAAAGAGGCC ATACAGAAAG CTGAGGCCAT TGATAGCGAC CCCTCCAGAA 
TGCAGCCTGG TAGGGCAGTG CGTGTGCGGA AAGCCAGGCC CTGCTCCCAG TACAACCACA 
AACTCTTCTC TGGAGACATG CTCTCCTTCA TTCAGAAGGA GAAGGCGGAT GTCCAGGACG 
CAGAGCTCAG GGCAGCAGAT CCCCCTGGTG GTTGAAAGCT GCATTCGCTT CATCAACCTG 
CATGGTCTTC ATCATGAAGG CATTTTTAGG GTTCCTGGAT CACAGACTGA AGTTAATCAT 
ATCAGGGATG CTTTTGAAAG AGGCGAAGAC CCACTCACTG ACAGCGAGAG TGACATTGAT 
TCAGTGGCGG GGGTCCTGAA GCTCTATTTC AGAGGACTGG AGAAACCTCT GTTCCCTGAG 
GAGAGCTTTA GCCAGCTCAT GGAGTGTGTC CAAATGGAAA ACATGACCGA AAAAGTAGCG 
CAGATAAAGT CAGTAGTGTC GTCCTATCCC AGACCTGTCA TCATAGTGAT GCGGTATCTC 
TTCGCCTTCC TTCATCACGT CTCTCAGTAC AGTGATGAGA ACATGATGCA GCCGTATAAT 
CTGGCTGTCT GCTTCGGGCC CAGTCTGCTC CGAGGCGTAG AGATGGGTGG TGACGAGGTG 
ACCCTGGCTC CTCAGATCAA TGAGCTGGTC AAAACCATGA TCCTTCATCA TGAGAACATC 
TTCCCAGGAC CTTCTGAACT TCCAGGCCCC GTTTATGAGA AGTGCATGAC TTTGGAGCAG 
GAGTACTGTG AACCCATCAC AGAGGAGGGC GAAGGAGACC CTGAACATCT TCCCAGTGAA 
GATGAGTGGG AGGCTGTGGC CATGTTTGAT TATGTTGCAC GCTCTGCAGC CGAGCTGTCA 
TTCAAGCAGG GTGAGCACCT CCTACTGCAC AGCAAGGCCT CAGCTGATTG GTGGAGAGGG 
GAAGTGGGAG GAATGAAAGG CTTGATCCCC CATAAGTACA TCAGTGTGGT AGAAGGGATG 
GAGCGTAAGG ACCGGGGAAA GAAGGAGGAC AGTAGAGGGG GAAGCACAGG AAACCTAGCA 
GAGGAGCAGC AGCAACAGTC TGAACACAGC ACTCGTATGC GAGTGAACAG CGACAGCGCA 
TCTTTGCCCG GCCGGCAGAG GACCGGCAGT GGAAGCAGCG GCAGCATTGG TGGAGGAAAC 
AGTGGGAATA TGAGCCCTGT ACGAAAGCTC ACCCTGCAGG TGCCTGAGGG ACGGCTCATG 
CTTCCTCCTC AGACCACTGC AGTCGCACGC CAATTCTCAG GTCATCAGGA GCGCAGACAC 
ACTTTAGACT CTCTGAGGCC AGTAGGAGCT CCAGACAGAC AGACAGTACA TATCGACAAG 
GAGGTCAGTC GTCAGATGAA CTCCGTGTTT AAGGAGCTGT TGTCTCGTCA GCCTCCTCAA 
GAGGCCTTGG GTTCGAGCGC TTCTCCATCC TCTCCTCCTG CTTCTGTCCC ATCCATCCCA 
TCGGTCTCCT CTGCTGCTGC TCGACCTCCG GTGAAGAAGG GAGGCTTTGG CCTGCGCAGC 
AGAGGCCTCT TCAAACCCCA GGACCAACAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295030.1
CDS	412..2007
Translation	MTYLKRPYRKLRPLIATPPECSLVGQCVCGKPGPAPSTTTNSSLETCSPSFRRRRRMSRTQSSGQQIPLVVESCIRFINLHGLHHEGIFRVPGSQTEVNHIRDAFERGEDPLTDSESDIDSVAGVLKLYFRGLEKPLFPEESFSQLMECVQMENMTEKVAQIKSVVSSYPRPVIIVMRYLFAFLHHVSQYSDENMMQPYNLAVCFGPSLLRGVEMGGDEVTLAPQINELVKTMILHHENIFPGPSELPGPVYEKCMTLEQEYCEPITEEGEGDPEHLPSEDEWEAVAMFDYVARSAAELSFKQGEHLLLHSKASADWWRGEVGGMKGLIPHKYISVVEGMERKDRGKKEDSRGGSTGNLAEEQQQQSEHSTRMRVNSDSASLPGRQRTGSGSSGSIGGGNSGNMSPVRKLTLQVPEGRLMLPPQTTAVARQFSGHQERRHTLDSLRPVGAPDRQTVHIDKEVSRQMNSVFKELLSRQPPQEALGSSASPSSPPASVPSIPSVSSAAARPPVKKGGFGLRSRGLFKPQDQQD

Target ORF information:

RefSeq Version	XM_009296755.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296755.1

ORF Insert Sequence:

ATGACCTACT TAAAGAGGCC ATACAGAAAG CTGAGGCCAT TGATAGCGAC CCCTCCAGAA 
TGCAGCCTGG TAGGGCAGTG CGTGTGCGGA AAGCCAGGCC CTGCTCCCAG TACAACCACA 
AACTCTTCTC TGGAGACATG CTCTCCTTCA TTCAGAAGGA GAAGGCGGAT GTCCAGGACG 
CAGAGCTCAG GGCAGCAGAT CCCCCTGGTG GTTGAAAGCT GCATTCGCTT CATCAACCTG 
CATGGTCTTC ATCATGAAGG CATTTTTAGG GTTCCTGGAT CACAGACTGA AGTTAATCAT 
ATCAGGGATG CTTTTGAAAG AGGCGAAGAC CCACTCACTG ACAGCGAGAG TGACATTGAT 
TCAGTGGCGG GGGTCCTGAA GCTCTATTTC AGAGGACTGG AGAAACCTCT GTTCCCTGAG 
GAGAGCTTTA GCCAGCTCAT GGAGTGTGTC CAAATGGAAA ACATGACCGA AAAAGTAGCG 
CAGATAAAGT CAGTAGTGTC GTCCTATCCC AGACCTGTCA TCATAGTGAT GCGGTATCTC 
TTCGCCTTCC TTCATCACGT CTCTCAGTAC AGTGATGAGA ACATGATGCA GCCGTATAAT 
CTGGCTGTCT GCTTCGGGCC CAGTCTGCTC CGAGGCGTAG AGATGGGTGG TGACGAGGTG 
ACCCTGGCTC CTCAGATCAA TGAGCTGGTC AAAACCATGA TCCTTCATCA TGAGAACATC 
TTCCCAGGAC CTTCTGAACT TCCAGGCCCC GTTTATGAGA AGTGCATGAC TTTGGAGCAG 
GAGTACTGTG AACCCATCAC AGAGGAGGGC GAAGGAGACC CTGAACATCT TCCCAGTGAA 
GATGAGTGGG AGGCTGTGGC CATGTTTGAT TATGTTGCAC GCTCTGCAGC CGAGCTGTCA 
TTCAAGCAGG GTGAGCACCT CCTACTGCAC AGCAAGGCCT CAGCTGATTG GTGGAGAGGG 
GAAGTGGGAG GAATGAAAGG CTTGATCCCC CATAAGTACA TCAGTGTGGT AGAAGGGATG 
GAGCGTAAGG ACCGGGGAAA GAAGGAGGAC AGTAGAGGGG GAAGCACAGG AAACCTAGCA 
GAGGAGCAGC AGCAACAGTC TGAACACAGC ACTCGTATGC GAGTGAACAG CGACAGCGCA 
TCTTTGCCCG GCCGGCAGAG GACCGGCAGT GGAAGCAGCG GCAGCATTGG TGGAGGAAAC 
AGTGGGAATA TGAGCCCTGT ACGAAAGCTC ACCCTGCAGG TGCCTGAGGG ACGGCTCATG 
CTTCCTCCTC AGACCACTGC AGTCGCACGC CAATTCTCAG GTCATCAGGA GCGCAGACAC 
ACTTTAGACT CTCTGAGGCC AGTAGGAGCT CCAGACAGAC AGACAGTACA TATCGACAAG 
GAGGTCAGTC GTCAGATGAA CTCCGTGTTT AAGGAGCTGT TGTCTCGTCA GCCTCCTCAA 
GAGGCCTTGG GTTCGAGCGC TTCTCCATCC TCTCCTCCTG CTTCTGTCCC ATCCATCCCA 
TCGGTCTCCT CTGCTGCTGC TCGACCTCCG GTGAAGAAGG GAGGCTTTGG CCTGCGCAGC 
AGAGGCCTCT TCAAACCCCA GGACCAACAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap4b ( XM_009296756.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295031.1 Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa43469
Clone ID Related Accession (Same CDS sequence)	XM_009296756.2
Accession Version	XM_009296756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 4 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAA GGAGAAGGCG 
GATGTCCAGG ACGCAGAGCT CAGGGCAGCA GATCCCCCTG GTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295031.1
CDS	742..2289
Translation	MTSHGKLRKEKGSLAEYETQIKEVRGQLSEQLKILDAQLEVKTQQLQDMSEYLRRRGEIEAEYARSLEKLSEKFTVKTKRKEQLDLSVAQCWSVLLTQTRQESRDHSSLSELCGNTLTQRLSHCIEDTHRLAKRSKEVGLQMQDELLKITTELQTALKTYHQYHIDCLAAEGKLKEATRLEEKQTGKSADLGLSQSGGQRRSSVKKMERLMEKRQGKVQETQLKCTKARNDYLLNLAAANASMNKYYLQDICTLIDCTDLGYHLSVTRVIRGYLSYKNRIVQNMKNGLQQLDTAVTGLNQGQDRDSLLQAHNIAFCLPFRFQYQPHEGDQVCEVSAEGQVRYELDTRFQQLQSRLASVTLETDEVSKTLKATHSNLLESVCDDDCNPAPDASSTPSVEGTADGTGTKPSLAKRRANQQDTETFYFTKVKEHVSGSSLISKLQAKHDLLKEAIQKAEAIDSDPSRMQPGRAVRVRKARPCSQYNHKLFSGDMLSFIQKEKADVQDAELRAADPPGG

Target ORF information:

RefSeq Version	XM_009296756.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296756.2

ORF Insert Sequence:

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAA GGAGAAGGCG 
GATGTCCAGG ACGCAGAGCT CAGGGCAGCA GATCCCCCTG GTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap4b ( XM_684637.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_689729.3 Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27626
Clone ID Related Accession (Same CDS sequence)	XM_684637.7 , XM_684637.6
Accession Version	XM_684637.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_684637.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAG CTCAGGGCAG 
CAGATCCCCC TGGTGGTTGA AAGCTGCATT CGCTTCATCA ACCTGCATGG TCTTCATCAT 
GAAGGCATTT TTAGGGTTCC TGGATCACAG ACTGAAGTTA ATCATATCAG GGATGCTTTT 
GAAAGAGGCG AAGACCCACT CACTGACAGC GAGAGTGACA TTGATTCAGT GGCGGGGGTC 
CTGAAGCTCT ATTTCAGAGG ACTGGAGAAA CCTCTGTTCC CTGAGGAGAG CTTTAGCCAG 
CTCATGGAGT GTGTCCAAAT GGAAAACATG ACCGAAAAAG TAGCGCAGAT AAAGTCAGTA 
GTGTCGTCCT ATCCCAGACC TGTCATCATA GTGATGCGGT ATCTCTTCGC CTTCCTTCAT 
CACGTCTCTC AGTACAGTGA TGAGAACATG ATGCAGCCGT ATAATCTGGC TGTCTGCTTC 
GGGCCCAGTC TGCTCCGAGG CGTAGAGATG GGTGGTGACG AGGTGACCCT GGCTCCTCAG 
ATCAATGAGC TGGTCAAAAC CATGATCCTT CATCATGAGA ACATCTTCCC AGGACCTTCT 
GAACTTCCAG GCCCCGTTTA TGAGAAGTGC ATGACTTTGG AGCAGGAGTA CTGTGAACCC 
ATCACAGAGG AGGGCGAAGG AGACCCTGAA CATCTTCCCA GTGAAGATGA GTGGGAGGCT 
GTGGCCATGT TTGATTATGT TGCACGCTCT GCAGCCGAGC TGTCATTCAA GCAGGGTGAG 
CACCTCCTAC TGCACAGCAA GGCCTCAGCT GATTGGTGGA GAGGGGAAGT GGGAGGAATG 
AAAGGCTTGA TCCCCCATAA GTACATCAGT GTGGTAGAAG GGATGGAGCG TAAGGACCGG 
GGAAAGAAGG AGGACAGTAG AGGGGGAAGC ACAGGAAACC TAGCAGAGGA GCAGCAGCAA 
CAGTCTGAAC ACAGCACTCG TATGCGAGTG AACAGCGACA GCGCATCTTT GCCCGGCCGG 
CAGAGGACCG GCAGTGGAAG CAGCGGCAGC ATTGGTGGAG GAAACAGTGG GAATATGAGC 
CCTGTACGAA AGCTCACCCT GCAGGTGCCT GAGGGACGGC TCATGCTTCC TCCTCAGACC 
ACTGCAGTCG CACGCCAATT CTCAGGTCAT CAGGAGCGCA GACACACTTT AGACTCTCTG 
AGGCCAGTAG GAGCTCCAGA CAGACAGACA GTACATATCG ACAAGGAGGT CAGTCGTCAG 
ATGAACTCCG TGTTTAAGGA GCTGTTGTCT CGTCAGCCTC CTCAAGAGGC CTTGGGTTCG 
AGCGCTTCTC CATCCTCTCC TCCTGCTTCT GTCCCATCCA TCCCATCGGT CTCCTCTGCT 
GCTGCTCGAC CTCCGGTGAA GAAGGGAGGC TTTGGCCTGC GCAGCAGAGG CCTCTTCAAA 
CCCCAGGACC AACAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_689729.3
CDS	743..3643
Translation	MTSHGKLRKEKGSLAEYETQIKEVRGQLSEQLKILDAQLEVKTQQLQDMSEYLRRRGEIEAEYARSLEKLSEKFTVKTKRKEQLDLSVAQCWSVLLTQTRQESRDHSSLSELCGNTLTQRLSHCIEDTHRLAKRSKEVGLQMQDELLKITTELQTALKTYHQYHIDCLAAEGKLKEATRLEEKQTGKSADLGLSQSGGQRRSSVKKMERLMEKRQGKVQETQLKCTKARNDYLLNLAAANASMNKYYLQDICTLIDCTDLGYHLSVTRVIRGYLSYKNRIVQNMKNGLQQLDTAVTGLNQGQDRDSLLQAHNIAFCLPFRFQYQPHEGDQVCEVSAEGQVRYELDTRFQQLQSRLASVTLETDEVSKTLKATHSNLLESVCDDDCNPAPDASSTPSVEGTADGTGTKPSLAKRRANQQDTETFYFTKVKEHVSGSSLISKLQAKHDLLKEAIQKAEAIDSDPSRMQPGRAVRVRKARPCSQYNHKLFSGDMLSFIQSSGQQIPLVVESCIRFINLHGLHHEGIFRVPGSQTEVNHIRDAFERGEDPLTDSESDIDSVAGVLKLYFRGLEKPLFPEESFSQLMECVQMENMTEKVAQIKSVVSSYPRPVIIVMRYLFAFLHHVSQYSDENMMQPYNLAVCFGPSLLRGVEMGGDEVTLAPQINELVKTMILHHENIFPGPSELPGPVYEKCMTLEQEYCEPITEEGEGDPEHLPSEDEWEAVAMFDYVARSAAELSFKQGEHLLLHSKASADWWRGEVGGMKGLIPHKYISVVEGMERKDRGKKEDSRGGSTGNLAEEQQQQSEHSTRMRVNSDSASLPGRQRTGSGSSGSIGGGNSGNMSPVRKLTLQVPEGRLMLPPQTTAVARQFSGHQERRHTLDSLRPVGAPDRQTVHIDKEVSRQMNSVFKELLSRQPPQEALGSSASPSSPPASVPSIPSVSSAAARPPVKKGGFGLRSRGLFKPQDQQD

Target ORF information:

RefSeq Version	XM_684637.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_684637.7

ORF Insert Sequence:

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAG CTCAGGGCAG 
CAGATCCCCC TGGTGGTTGA AAGCTGCATT CGCTTCATCA ACCTGCATGG TCTTCATCAT 
GAAGGCATTT TTAGGGTTCC TGGATCACAG ACTGAAGTTA ATCATATCAG GGATGCTTTT 
GAAAGAGGCG AAGACCCACT CACTGACAGC GAGAGTGACA TTGATTCAGT GGCGGGGGTC 
CTGAAGCTCT ATTTCAGAGG ACTGGAGAAA CCTCTGTTCC CTGAGGAGAG CTTTAGCCAG 
CTCATGGAGT GTGTCCAAAT GGAAAACATG ACCGAAAAAG TAGCGCAGAT AAAGTCAGTA 
GTGTCGTCCT ATCCCAGACC TGTCATCATA GTGATGCGGT ATCTCTTCGC CTTCCTTCAT 
CACGTCTCTC AGTACAGTGA TGAGAACATG ATGCAGCCGT ATAATCTGGC TGTCTGCTTC 
GGGCCCAGTC TGCTCCGAGG CGTAGAGATG GGTGGTGACG AGGTGACCCT GGCTCCTCAG 
ATCAATGAGC TGGTCAAAAC CATGATCCTT CATCATGAGA ACATCTTCCC AGGACCTTCT 
GAACTTCCAG GCCCCGTTTA TGAGAAGTGC ATGACTTTGG AGCAGGAGTA CTGTGAACCC 
ATCACAGAGG AGGGCGAAGG AGACCCTGAA CATCTTCCCA GTGAAGATGA GTGGGAGGCT 
GTGGCCATGT TTGATTATGT TGCACGCTCT GCAGCCGAGC TGTCATTCAA GCAGGGTGAG 
CACCTCCTAC TGCACAGCAA GGCCTCAGCT GATTGGTGGA GAGGGGAAGT GGGAGGAATG 
AAAGGCTTGA TCCCCCATAA GTACATCAGT GTGGTAGAAG GGATGGAGCG TAAGGACCGG 
GGAAAGAAGG AGGACAGTAG AGGGGGAAGC ACAGGAAACC TAGCAGAGGA GCAGCAGCAA 
CAGTCTGAAC ACAGCACTCG TATGCGAGTG AACAGCGACA GCGCATCTTT GCCCGGCCGG 
CAGAGGACCG GCAGTGGAAG CAGCGGCAGC ATTGGTGGAG GAAACAGTGG GAATATGAGC 
CCTGTACGAA AGCTCACCCT GCAGGTGCCT GAGGGACGGC TCATGCTTCC TCCTCAGACC 
ACTGCAGTCG CACGCCAATT CTCAGGTCAT CAGGAGCGCA GACACACTTT AGACTCTCTG 
AGGCCAGTAG GAGCTCCAGA CAGACAGACA GTACATATCG ACAAGGAGGT CAGTCGTCAG 
ATGAACTCCG TGTTTAAGGA GCTGTTGTCT CGTCAGCCTC CTCAAGAGGC CTTGGGTTCG 
AGCGCTTCTC CATCCTCTCC TCCTGCTTCT GTCCCATCCA TCCCATCGGT CTCCTCTGCT 
GCTGCTCGAC CTCCGGTGAA GAAGGGAGGC TTTGGCCTGC GCAGCAGAGG CCTCTTCAAA 
CCCCAGGACC AACAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap4b ( XM_684637.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_689729.3 Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27626
Clone ID Related Accession (Same CDS sequence)	XM_684637.7 , XM_684637.6
Accession Version	XM_684637.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_684637.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAG CTCAGGGCAG 
CAGATCCCCC TGGTGGTTGA AAGCTGCATT CGCTTCATCA ACCTGCATGG TCTTCATCAT 
GAAGGCATTT TTAGGGTTCC TGGATCACAG ACTGAAGTTA ATCATATCAG GGATGCTTTT 
GAAAGAGGCG AAGACCCACT CACTGACAGC GAGAGTGACA TTGATTCAGT GGCGGGGGTC 
CTGAAGCTCT ATTTCAGAGG ACTGGAGAAA CCTCTGTTCC CTGAGGAGAG CTTTAGCCAG 
CTCATGGAGT GTGTCCAAAT GGAAAACATG ACCGAAAAAG TAGCGCAGAT AAAGTCAGTA 
GTGTCGTCCT ATCCCAGACC TGTCATCATA GTGATGCGGT ATCTCTTCGC CTTCCTTCAT 
CACGTCTCTC AGTACAGTGA TGAGAACATG ATGCAGCCGT ATAATCTGGC TGTCTGCTTC 
GGGCCCAGTC TGCTCCGAGG CGTAGAGATG GGTGGTGACG AGGTGACCCT GGCTCCTCAG 
ATCAATGAGC TGGTCAAAAC CATGATCCTT CATCATGAGA ACATCTTCCC AGGACCTTCT 
GAACTTCCAG GCCCCGTTTA TGAGAAGTGC ATGACTTTGG AGCAGGAGTA CTGTGAACCC 
ATCACAGAGG AGGGCGAAGG AGACCCTGAA CATCTTCCCA GTGAAGATGA GTGGGAGGCT 
GTGGCCATGT TTGATTATGT TGCACGCTCT GCAGCCGAGC TGTCATTCAA GCAGGGTGAG 
CACCTCCTAC TGCACAGCAA GGCCTCAGCT GATTGGTGGA GAGGGGAAGT GGGAGGAATG 
AAAGGCTTGA TCCCCCATAA GTACATCAGT GTGGTAGAAG GGATGGAGCG TAAGGACCGG 
GGAAAGAAGG AGGACAGTAG AGGGGGAAGC ACAGGAAACC TAGCAGAGGA GCAGCAGCAA 
CAGTCTGAAC ACAGCACTCG TATGCGAGTG AACAGCGACA GCGCATCTTT GCCCGGCCGG 
CAGAGGACCG GCAGTGGAAG CAGCGGCAGC ATTGGTGGAG GAAACAGTGG GAATATGAGC 
CCTGTACGAA AGCTCACCCT GCAGGTGCCT GAGGGACGGC TCATGCTTCC TCCTCAGACC 
ACTGCAGTCG CACGCCAATT CTCAGGTCAT CAGGAGCGCA GACACACTTT AGACTCTCTG 
AGGCCAGTAG GAGCTCCAGA CAGACAGACA GTACATATCG ACAAGGAGGT CAGTCGTCAG 
ATGAACTCCG TGTTTAAGGA GCTGTTGTCT CGTCAGCCTC CTCAAGAGGC CTTGGGTTCG 
AGCGCTTCTC CATCCTCTCC TCCTGCTTCT GTCCCATCCA TCCCATCGGT CTCCTCTGCT 
GCTGCTCGAC CTCCGGTGAA GAAGGGAGGC TTTGGCCTGC GCAGCAGAGG CCTCTTCAAA 
CCCCAGGACC AACAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_689729.3
CDS	742..3642
Translation	MTSHGKLRKEKGSLAEYETQIKEVRGQLSEQLKILDAQLEVKTQQLQDMSEYLRRRGEIEAEYARSLEKLSEKFTVKTKRKEQLDLSVAQCWSVLLTQTRQESRDHSSLSELCGNTLTQRLSHCIEDTHRLAKRSKEVGLQMQDELLKITTELQTALKTYHQYHIDCLAAEGKLKEATRLEEKQTGKSADLGLSQSGGQRRSSVKKMERLMEKRQGKVQETQLKCTKARNDYLLNLAAANASMNKYYLQDICTLIDCTDLGYHLSVTRVIRGYLSYKNRIVQNMKNGLQQLDTAVTGLNQGQDRDSLLQAHNIAFCLPFRFQYQPHEGDQVCEVSAEGQVRYELDTRFQQLQSRLASVTLETDEVSKTLKATHSNLLESVCDDDCNPAPDASSTPSVEGTADGTGTKPSLAKRRANQQDTETFYFTKVKEHVSGSSLISKLQAKHDLLKEAIQKAEAIDSDPSRMQPGRAVRVRKARPCSQYNHKLFSGDMLSFIQSSGQQIPLVVESCIRFINLHGLHHEGIFRVPGSQTEVNHIRDAFERGEDPLTDSESDIDSVAGVLKLYFRGLEKPLFPEESFSQLMECVQMENMTEKVAQIKSVVSSYPRPVIIVMRYLFAFLHHVSQYSDENMMQPYNLAVCFGPSLLRGVEMGGDEVTLAPQINELVKTMILHHENIFPGPSELPGPVYEKCMTLEQEYCEPITEEGEGDPEHLPSEDEWEAVAMFDYVARSAAELSFKQGEHLLLHSKASADWWRGEVGGMKGLIPHKYISVVEGMERKDRGKKEDSRGGSTGNLAEEQQQQSEHSTRMRVNSDSASLPGRQRTGSGSSGSIGGGNSGNMSPVRKLTLQVPEGRLMLPPQTTAVARQFSGHQERRHTLDSLRPVGAPDRQTVHIDKEVSRQMNSVFKELLSRQPPQEALGSSASPSSPPASVPSIPSVSSAAARPPVKKGGFGLRSRGLFKPQDQQD

Target ORF information:

RefSeq Version	XM_684637.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_684637.6

ORF Insert Sequence:

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAG CTCAGGGCAG 
CAGATCCCCC TGGTGGTTGA AAGCTGCATT CGCTTCATCA ACCTGCATGG TCTTCATCAT 
GAAGGCATTT TTAGGGTTCC TGGATCACAG ACTGAAGTTA ATCATATCAG GGATGCTTTT 
GAAAGAGGCG AAGACCCACT CACTGACAGC GAGAGTGACA TTGATTCAGT GGCGGGGGTC 
CTGAAGCTCT ATTTCAGAGG ACTGGAGAAA CCTCTGTTCC CTGAGGAGAG CTTTAGCCAG 
CTCATGGAGT GTGTCCAAAT GGAAAACATG ACCGAAAAAG TAGCGCAGAT AAAGTCAGTA 
GTGTCGTCCT ATCCCAGACC TGTCATCATA GTGATGCGGT ATCTCTTCGC CTTCCTTCAT 
CACGTCTCTC AGTACAGTGA TGAGAACATG ATGCAGCCGT ATAATCTGGC TGTCTGCTTC 
GGGCCCAGTC TGCTCCGAGG CGTAGAGATG GGTGGTGACG AGGTGACCCT GGCTCCTCAG 
ATCAATGAGC TGGTCAAAAC CATGATCCTT CATCATGAGA ACATCTTCCC AGGACCTTCT 
GAACTTCCAG GCCCCGTTTA TGAGAAGTGC ATGACTTTGG AGCAGGAGTA CTGTGAACCC 
ATCACAGAGG AGGGCGAAGG AGACCCTGAA CATCTTCCCA GTGAAGATGA GTGGGAGGCT 
GTGGCCATGT TTGATTATGT TGCACGCTCT GCAGCCGAGC TGTCATTCAA GCAGGGTGAG 
CACCTCCTAC TGCACAGCAA GGCCTCAGCT GATTGGTGGA GAGGGGAAGT GGGAGGAATG 
AAAGGCTTGA TCCCCCATAA GTACATCAGT GTGGTAGAAG GGATGGAGCG TAAGGACCGG 
GGAAAGAAGG AGGACAGTAG AGGGGGAAGC ACAGGAAACC TAGCAGAGGA GCAGCAGCAA 
CAGTCTGAAC ACAGCACTCG TATGCGAGTG AACAGCGACA GCGCATCTTT GCCCGGCCGG 
CAGAGGACCG GCAGTGGAAG CAGCGGCAGC ATTGGTGGAG GAAACAGTGG GAATATGAGC 
CCTGTACGAA AGCTCACCCT GCAGGTGCCT GAGGGACGGC TCATGCTTCC TCCTCAGACC 
ACTGCAGTCG CACGCCAATT CTCAGGTCAT CAGGAGCGCA GACACACTTT AGACTCTCTG 
AGGCCAGTAG GAGCTCCAGA CAGACAGACA GTACATATCG ACAAGGAGGT CAGTCGTCAG 
ATGAACTCCG TGTTTAAGGA GCTGTTGTCT CGTCAGCCTC CTCAAGAGGC CTTGGGTTCG 
AGCGCTTCTC CATCCTCTCC TCCTGCTTCT GTCCCATCCA TCCCATCGGT CTCCTCTGCT 
GCTGCTCGAC CTCCGGTGAA GAAGGGAGGC TTTGGCCTGC GCAGCAGAGG CCTCTTCAAA 
CCCCAGGACC AACAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap4b ( XM_005162108.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162165.1 Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa27627
Clone ID Related Accession (Same CDS sequence)	XM_005162108.2
Accession Version	XM_005162108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005162108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAAGGAGAAG GCGGATGTCC AGGACGCAGA GCTCAGGGCA GCAGATCCCC 
CTGGTGGTTG AAAGCTGCAT TCGCTTCATC AACCTGCATG GTCTTCATCA TGAAGGCATT 
TTTAGGGTTC CTGGATCACA GACTGAAGTT AATCATATCA GGGATGCTTT TGAAAGAGGC 
GAAGACCCAC TCACTGACAG CGAGAGTGAC ATTGATTCAG TGGCGGGGGT CCTGAAGCTC 
TATTTCAGAG GACTGGAGAA ACCTCTGTTC CCTGAGGAGA GCTTTAGCCA GCTCATGGAG 
TGTGTCCAAA TGGAAAACAT GACCGAAAAA GTAGCGCAGA TAAAGTCAGT AGTGTCGTCC 
TATCCCAGAC CTGTCATCAT AGTGATGCGG TATCTCTTCG CCTTCCTTCA TCACGTCTCT 
CAGTACAGTG ATGAGAACAT GATGCAGCCG TATAATCTGG CTGTCTGCTT CGGGCCCAGT 
CTGCTCCGAG GCGTAGAGAT GGGTGGTGAC GAGGTGACCC TGGCTCCTCA GATCAATGAG 
CTGGTCAAAA CCATGATCCT TCATCATGAG AACATCTTCC CAGGACCTTC TGAACTTCCA 
GGCCCCGTTT ATGAGAAGTG CATGACTTTG GAGCAGGAGT ACTGTGAACC CATCACAGAG 
GAGGGCGAAG GAGACCCTGA ACATCTTCCC AGTGAAGATG AGTGGGAGGC TGTGGCCATG 
TTTGATTATG TTGCACGCTC TGCAGCCGAG CTGTCATTCA AGCAGGGTGA GCACCTCCTA 
CTGCACAGCA AGGCCTCAGC TGATTGGTGG AGAGGGGAAG TGGGAGGAAT GAAAGGCTTG 
ATCCCCCATA AGTACATCAG TGTGGTAGAA GGGATGGAGC GTAAGGACCG GGGAAAGAAG 
GAGGACAGTA GAGGGGGAAG CACAGGAAAC CTAGCAGAGG AGCAGCAGCA ACAGTCTGAA 
CACAGCACTC GTATGCGAGT GAACAGCGAC AGCGCATCTT TGCCCGGCCG GCAGAGGACC 
GGCAGTGGAA GCAGCGGCAG CATTGGTGGA GGAAACAGTG GGAATATGAG CCCTGTACGA 
AAGCTCACCC TGCAGGTGCC TGAGGGACGG CTCATGCTTC CTCCTCAGAC CACTGCAGTC 
GCACGCCAAT TCTCAGGTCA TCAGGAGCGC AGACACACTT TAGACTCTCT GAGGCCAGTA 
GGAGCTCCAG ACAGACAGAC AGTACATATC GACAAGGAGG TCAGTCGTCA GATGAACTCC 
GTGTTTAAGG AGCTGTTGTC TCGTCAGCCT CCTCAAGAGG CCTTGGGTTC GAGCGCTTCT 
CCATCCTCTC CTCCTGCTTC TGTCCCATCC ATCCCATCGG TCTCCTCTGC TGCTGCTCGA 
CCTCCGGTGA AGAAGGGAGG CTTTGGCCTG CGCAGCAGAG GCCTCTTCAA ACCCCAGGAC 
CAACAGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162165.1
CDS	742..3573
Translation	MTSHGKLRKEKGSLAEYETQIKEVRGQLSEQLKILDAQLEVKTQQLQDMSEYLRRRGEIEAEYARSLEKLSEKFTVKTKRKEQLDLSVAQCWSVLLTQTRQESRDHSSLSELCGNTLTQRLSHCIEDTHRLAKRSKEVGLQMQDELLKITTELQTALKTYHQYHIDCLAAEGKLKEATRLEEKQTGKSADLGLSQSGGQRRSSVKKMERLMEKRQGKVQETQLKCTKARNDYLLNLAAANASMNKYYLQDICTLIDCTDLGYHLSVTRVIRGYLSYKNRIVQNMKNGLQQLDTAVTGLNQGQDRDSLLQAHNIAFCLPFRFQYQPHEGDQVCEVSAEGQVRYELDTRFQQLQSRLASVTLETDEVSKTLKATHSNLLESVCDDDCNPAPDASSTPSVEGTADGTGTKPSLAKRRANQQDTETFYFTKVKEHVSGSSLISKLQAKHDLLKEAIQKAEAIDSDPSRRRRRMSRTQSSGQQIPLVVESCIRFINLHGLHHEGIFRVPGSQTEVNHIRDAFERGEDPLTDSESDIDSVAGVLKLYFRGLEKPLFPEESFSQLMECVQMENMTEKVAQIKSVVSSYPRPVIIVMRYLFAFLHHVSQYSDENMMQPYNLAVCFGPSLLRGVEMGGDEVTLAPQINELVKTMILHHENIFPGPSELPGPVYEKCMTLEQEYCEPITEEGEGDPEHLPSEDEWEAVAMFDYVARSAAELSFKQGEHLLLHSKASADWWRGEVGGMKGLIPHKYISVVEGMERKDRGKKEDSRGGSTGNLAEEQQQQSEHSTRMRVNSDSASLPGRQRTGSGSSGSIGGGNSGNMSPVRKLTLQVPEGRLMLPPQTTAVARQFSGHQERRHTLDSLRPVGAPDRQTVHIDKEVSRQMNSVFKELLSRQPPQEALGSSASPSSPPASVPSIPSVSSAAARPPVKKGGFGLRSRGLFKPQDQQD

Target ORF information:

RefSeq Version	XM_005162108.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162108.2

ORF Insert Sequence:

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAAGGAGAAG GCGGATGTCC AGGACGCAGA GCTCAGGGCA GCAGATCCCC 
CTGGTGGTTG AAAGCTGCAT TCGCTTCATC AACCTGCATG GTCTTCATCA TGAAGGCATT 
TTTAGGGTTC CTGGATCACA GACTGAAGTT AATCATATCA GGGATGCTTT TGAAAGAGGC 
GAAGACCCAC TCACTGACAG CGAGAGTGAC ATTGATTCAG TGGCGGGGGT CCTGAAGCTC 
TATTTCAGAG GACTGGAGAA ACCTCTGTTC CCTGAGGAGA GCTTTAGCCA GCTCATGGAG 
TGTGTCCAAA TGGAAAACAT GACCGAAAAA GTAGCGCAGA TAAAGTCAGT AGTGTCGTCC 
TATCCCAGAC CTGTCATCAT AGTGATGCGG TATCTCTTCG CCTTCCTTCA TCACGTCTCT 
CAGTACAGTG ATGAGAACAT GATGCAGCCG TATAATCTGG CTGTCTGCTT CGGGCCCAGT 
CTGCTCCGAG GCGTAGAGAT GGGTGGTGAC GAGGTGACCC TGGCTCCTCA GATCAATGAG 
CTGGTCAAAA CCATGATCCT TCATCATGAG AACATCTTCC CAGGACCTTC TGAACTTCCA 
GGCCCCGTTT ATGAGAAGTG CATGACTTTG GAGCAGGAGT ACTGTGAACC CATCACAGAG 
GAGGGCGAAG GAGACCCTGA ACATCTTCCC AGTGAAGATG AGTGGGAGGC TGTGGCCATG 
TTTGATTATG TTGCACGCTC TGCAGCCGAG CTGTCATTCA AGCAGGGTGA GCACCTCCTA 
CTGCACAGCA AGGCCTCAGC TGATTGGTGG AGAGGGGAAG TGGGAGGAAT GAAAGGCTTG 
ATCCCCCATA AGTACATCAG TGTGGTAGAA GGGATGGAGC GTAAGGACCG GGGAAAGAAG 
GAGGACAGTA GAGGGGGAAG CACAGGAAAC CTAGCAGAGG AGCAGCAGCA ACAGTCTGAA 
CACAGCACTC GTATGCGAGT GAACAGCGAC AGCGCATCTT TGCCCGGCCG GCAGAGGACC 
GGCAGTGGAA GCAGCGGCAG CATTGGTGGA GGAAACAGTG GGAATATGAG CCCTGTACGA 
AAGCTCACCC TGCAGGTGCC TGAGGGACGG CTCATGCTTC CTCCTCAGAC CACTGCAGTC 
GCACGCCAAT TCTCAGGTCA TCAGGAGCGC AGACACACTT TAGACTCTCT GAGGCCAGTA 
GGAGCTCCAG ACAGACAGAC AGTACATATC GACAAGGAGG TCAGTCGTCA GATGAACTCC 
GTGTTTAAGG AGCTGTTGTC TCGTCAGCCT CCTCAAGAGG CCTTGGGTTC GAGCGCTTCT 
CCATCCTCTC CTCCTGCTTC TGTCCCATCC ATCCCATCGG TCTCCTCTGC TGCTGCTCGA 
CCTCCGGTGA AGAAGGGAGG CTTTGGCCTG CGCAGCAGAG GCCTCTTCAA ACCCCAGGAC 
CAACAGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap4b ( XM_009296754.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295029.1 Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43467
Clone ID Related Accession (Same CDS sequence)	XM_009296754.2 , XM_009296754.1
Accession Version	XM_009296754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAG CTCAGGGCAG 
CAGATCCCCC TGGTGGTTGA AAGCTGCATT CGCTTCATCA ACCTGCATGG TCTTCATCAT 
GAAGGCATTT TTAGGGTTCC TGGATCACAG ACTGAAGTTA ATCATATCAG GGATGCTTTT 
GAAAGAGGCG AAGACCCACT CACTGACAGC GAGAGTGACA TTGATTCAGT GGCGGGGGTC 
CTGAAGCTCT ATTTCAGAGG ACTGGAGAAA CCTCTGTTCC CTGAGGAGAG CTTTAGCCAG 
CTCATGGAGT GTGTCCAAAT GGAAAACATG ACCGAAAAAG TAGCGCAGAT AAAGTCAGTA 
GTGTCGTCCT ATCCCAGACC TGTCATCATA GTGATGCGGT ATCTCTTCGC CTTCCTTCAT 
CACGTCTCTC AGTACAGTGA TGAGAACATG ATGCAGCCGT ATAATCTGGC TGTCTGCTTC 
GGGCCCAGTC TGCTCCGAGG CGTAGAGATG GGTGGTGACG AGGTGACCCT GGCTCCTCAG 
ATCAATGAGC TGGTCAAAAC CATGATCCTT CATCATGAGA ACATCTTCCC AGGACCTTCT 
GAACTTCCAG GCCCCGTTTA TGAGAAGTGC ATGACTTTGG AGCAGGAGTA CTGTGAACCC 
ATCACAGAGG AGGGCGAAGG AGACCCTGAA CATCTTCCCA GTGAAGATGC CGAGCTGTCA 
TTCAAGCAGG GTGAGCACCT CCTACTGCAC AGCAAGGCCT CAGCTGATTG GTGGAGAGGG 
GAAGTGGGAG GAATGAAAGG CTTGATCCCC CATAAGTACA TCAGTGTGGT AGAAGGGATG 
GAGCGTAAGG ACCGGGGAAA GAAGGAGGAC AGTAGAGGGG GAAGCACAGG AAACCTAGCA 
GAGGAGCAGC AGCAACAGTC TGAACACAGC ACTCGTATGC GAGTGAACAG CGACAGCGCA 
TCTTTGCCCG GCCGGCAGAG GACCGGCAGT GGAAGCAGCG GCAGCATTGG TGGAGGAAAC 
AGTGGGAATA TGAGCCCTGT ACGAAAGCTC ACCCTGCAGG TGCCTGAGGG ACGGCTCATG 
CTTCCTCCTC AGACCACTGC AGTCGCACGC CAATTCTCAG GTCATCAGGA GCGCAGACAC 
ACTTTAGACT CTCTGAGGCC AGTAGGAGCT CCAGACAGAC AGACAGTACA TATCGACAAG 
GAGGTCAGTC GTCAGATGAA CTCCGTGTTT AAGGAGCTGT TGTCTCGTCA GCCTCCTCAA 
GAGGCCTTGG GTTCGAGCGC TTCTCCATCC TCTCCTCCTG CTTCTGTCCC ATCCATCCCA 
TCGGTCTCCT CTGCTGCTGC TCGACCTCCG GTGAAGAAGG GAGGCTTTGG CCTGCGCAGC 
AGAGGCCTCT TCAAACCCCA GGACCAACAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295029.1
CDS	743..3598
Translation	MTSHGKLRKEKGSLAEYETQIKEVRGQLSEQLKILDAQLEVKTQQLQDMSEYLRRRGEIEAEYARSLEKLSEKFTVKTKRKEQLDLSVAQCWSVLLTQTRQESRDHSSLSELCGNTLTQRLSHCIEDTHRLAKRSKEVGLQMQDELLKITTELQTALKTYHQYHIDCLAAEGKLKEATRLEEKQTGKSADLGLSQSGGQRRSSVKKMERLMEKRQGKVQETQLKCTKARNDYLLNLAAANASMNKYYLQDICTLIDCTDLGYHLSVTRVIRGYLSYKNRIVQNMKNGLQQLDTAVTGLNQGQDRDSLLQAHNIAFCLPFRFQYQPHEGDQVCEVSAEGQVRYELDTRFQQLQSRLASVTLETDEVSKTLKATHSNLLESVCDDDCNPAPDASSTPSVEGTADGTGTKPSLAKRRANQQDTETFYFTKVKEHVSGSSLISKLQAKHDLLKEAIQKAEAIDSDPSRMQPGRAVRVRKARPCSQYNHKLFSGDMLSFIQSSGQQIPLVVESCIRFINLHGLHHEGIFRVPGSQTEVNHIRDAFERGEDPLTDSESDIDSVAGVLKLYFRGLEKPLFPEESFSQLMECVQMENMTEKVAQIKSVVSSYPRPVIIVMRYLFAFLHHVSQYSDENMMQPYNLAVCFGPSLLRGVEMGGDEVTLAPQINELVKTMILHHENIFPGPSELPGPVYEKCMTLEQEYCEPITEEGEGDPEHLPSEDAELSFKQGEHLLLHSKASADWWRGEVGGMKGLIPHKYISVVEGMERKDRGKKEDSRGGSTGNLAEEQQQQSEHSTRMRVNSDSASLPGRQRTGSGSSGSIGGGNSGNMSPVRKLTLQVPEGRLMLPPQTTAVARQFSGHQERRHTLDSLRPVGAPDRQTVHIDKEVSRQMNSVFKELLSRQPPQEALGSSASPSSPPASVPSIPSVSSAAARPPVKKGGFGLRSRGLFKPQDQQD

Target ORF information:

RefSeq Version	XM_009296754.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296754.2

ORF Insert Sequence:

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAG CTCAGGGCAG 
CAGATCCCCC TGGTGGTTGA AAGCTGCATT CGCTTCATCA ACCTGCATGG TCTTCATCAT 
GAAGGCATTT TTAGGGTTCC TGGATCACAG ACTGAAGTTA ATCATATCAG GGATGCTTTT 
GAAAGAGGCG AAGACCCACT CACTGACAGC GAGAGTGACA TTGATTCAGT GGCGGGGGTC 
CTGAAGCTCT ATTTCAGAGG ACTGGAGAAA CCTCTGTTCC CTGAGGAGAG CTTTAGCCAG 
CTCATGGAGT GTGTCCAAAT GGAAAACATG ACCGAAAAAG TAGCGCAGAT AAAGTCAGTA 
GTGTCGTCCT ATCCCAGACC TGTCATCATA GTGATGCGGT ATCTCTTCGC CTTCCTTCAT 
CACGTCTCTC AGTACAGTGA TGAGAACATG ATGCAGCCGT ATAATCTGGC TGTCTGCTTC 
GGGCCCAGTC TGCTCCGAGG CGTAGAGATG GGTGGTGACG AGGTGACCCT GGCTCCTCAG 
ATCAATGAGC TGGTCAAAAC CATGATCCTT CATCATGAGA ACATCTTCCC AGGACCTTCT 
GAACTTCCAG GCCCCGTTTA TGAGAAGTGC ATGACTTTGG AGCAGGAGTA CTGTGAACCC 
ATCACAGAGG AGGGCGAAGG AGACCCTGAA CATCTTCCCA GTGAAGATGC CGAGCTGTCA 
TTCAAGCAGG GTGAGCACCT CCTACTGCAC AGCAAGGCCT CAGCTGATTG GTGGAGAGGG 
GAAGTGGGAG GAATGAAAGG CTTGATCCCC CATAAGTACA TCAGTGTGGT AGAAGGGATG 
GAGCGTAAGG ACCGGGGAAA GAAGGAGGAC AGTAGAGGGG GAAGCACAGG AAACCTAGCA 
GAGGAGCAGC AGCAACAGTC TGAACACAGC ACTCGTATGC GAGTGAACAG CGACAGCGCA 
TCTTTGCCCG GCCGGCAGAG GACCGGCAGT GGAAGCAGCG GCAGCATTGG TGGAGGAAAC 
AGTGGGAATA TGAGCCCTGT ACGAAAGCTC ACCCTGCAGG TGCCTGAGGG ACGGCTCATG 
CTTCCTCCTC AGACCACTGC AGTCGCACGC CAATTCTCAG GTCATCAGGA GCGCAGACAC 
ACTTTAGACT CTCTGAGGCC AGTAGGAGCT CCAGACAGAC AGACAGTACA TATCGACAAG 
GAGGTCAGTC GTCAGATGAA CTCCGTGTTT AAGGAGCTGT TGTCTCGTCA GCCTCCTCAA 
GAGGCCTTGG GTTCGAGCGC TTCTCCATCC TCTCCTCCTG CTTCTGTCCC ATCCATCCCA 
TCGGTCTCCT CTGCTGCTGC TCGACCTCCG GTGAAGAAGG GAGGCTTTGG CCTGCGCAGC 
AGAGGCCTCT TCAAACCCCA GGACCAACAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap4b ( XM_009296754.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295029.1 Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43467
Clone ID Related Accession (Same CDS sequence)	XM_009296754.2 , XM_009296754.1
Accession Version	XM_009296754.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAG CTCAGGGCAG 
CAGATCCCCC TGGTGGTTGA AAGCTGCATT CGCTTCATCA ACCTGCATGG TCTTCATCAT 
GAAGGCATTT TTAGGGTTCC TGGATCACAG ACTGAAGTTA ATCATATCAG GGATGCTTTT 
GAAAGAGGCG AAGACCCACT CACTGACAGC GAGAGTGACA TTGATTCAGT GGCGGGGGTC 
CTGAAGCTCT ATTTCAGAGG ACTGGAGAAA CCTCTGTTCC CTGAGGAGAG CTTTAGCCAG 
CTCATGGAGT GTGTCCAAAT GGAAAACATG ACCGAAAAAG TAGCGCAGAT AAAGTCAGTA 
GTGTCGTCCT ATCCCAGACC TGTCATCATA GTGATGCGGT ATCTCTTCGC CTTCCTTCAT 
CACGTCTCTC AGTACAGTGA TGAGAACATG ATGCAGCCGT ATAATCTGGC TGTCTGCTTC 
GGGCCCAGTC TGCTCCGAGG CGTAGAGATG GGTGGTGACG AGGTGACCCT GGCTCCTCAG 
ATCAATGAGC TGGTCAAAAC CATGATCCTT CATCATGAGA ACATCTTCCC AGGACCTTCT 
GAACTTCCAG GCCCCGTTTA TGAGAAGTGC ATGACTTTGG AGCAGGAGTA CTGTGAACCC 
ATCACAGAGG AGGGCGAAGG AGACCCTGAA CATCTTCCCA GTGAAGATGC CGAGCTGTCA 
TTCAAGCAGG GTGAGCACCT CCTACTGCAC AGCAAGGCCT CAGCTGATTG GTGGAGAGGG 
GAAGTGGGAG GAATGAAAGG CTTGATCCCC CATAAGTACA TCAGTGTGGT AGAAGGGATG 
GAGCGTAAGG ACCGGGGAAA GAAGGAGGAC AGTAGAGGGG GAAGCACAGG AAACCTAGCA 
GAGGAGCAGC AGCAACAGTC TGAACACAGC ACTCGTATGC GAGTGAACAG CGACAGCGCA 
TCTTTGCCCG GCCGGCAGAG GACCGGCAGT GGAAGCAGCG GCAGCATTGG TGGAGGAAAC 
AGTGGGAATA TGAGCCCTGT ACGAAAGCTC ACCCTGCAGG TGCCTGAGGG ACGGCTCATG 
CTTCCTCCTC AGACCACTGC AGTCGCACGC CAATTCTCAG GTCATCAGGA GCGCAGACAC 
ACTTTAGACT CTCTGAGGCC AGTAGGAGCT CCAGACAGAC AGACAGTACA TATCGACAAG 
GAGGTCAGTC GTCAGATGAA CTCCGTGTTT AAGGAGCTGT TGTCTCGTCA GCCTCCTCAA 
GAGGCCTTGG GTTCGAGCGC TTCTCCATCC TCTCCTCCTG CTTCTGTCCC ATCCATCCCA 
TCGGTCTCCT CTGCTGCTGC TCGACCTCCG GTGAAGAAGG GAGGCTTTGG CCTGCGCAGC 
AGAGGCCTCT TCAAACCCCA GGACCAACAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295029.1
CDS	742..3597
Translation	MTSHGKLRKEKGSLAEYETQIKEVRGQLSEQLKILDAQLEVKTQQLQDMSEYLRRRGEIEAEYARSLEKLSEKFTVKTKRKEQLDLSVAQCWSVLLTQTRQESRDHSSLSELCGNTLTQRLSHCIEDTHRLAKRSKEVGLQMQDELLKITTELQTALKTYHQYHIDCLAAEGKLKEATRLEEKQTGKSADLGLSQSGGQRRSSVKKMERLMEKRQGKVQETQLKCTKARNDYLLNLAAANASMNKYYLQDICTLIDCTDLGYHLSVTRVIRGYLSYKNRIVQNMKNGLQQLDTAVTGLNQGQDRDSLLQAHNIAFCLPFRFQYQPHEGDQVCEVSAEGQVRYELDTRFQQLQSRLASVTLETDEVSKTLKATHSNLLESVCDDDCNPAPDASSTPSVEGTADGTGTKPSLAKRRANQQDTETFYFTKVKEHVSGSSLISKLQAKHDLLKEAIQKAEAIDSDPSRMQPGRAVRVRKARPCSQYNHKLFSGDMLSFIQSSGQQIPLVVESCIRFINLHGLHHEGIFRVPGSQTEVNHIRDAFERGEDPLTDSESDIDSVAGVLKLYFRGLEKPLFPEESFSQLMECVQMENMTEKVAQIKSVVSSYPRPVIIVMRYLFAFLHHVSQYSDENMMQPYNLAVCFGPSLLRGVEMGGDEVTLAPQINELVKTMILHHENIFPGPSELPGPVYEKCMTLEQEYCEPITEEGEGDPEHLPSEDAELSFKQGEHLLLHSKASADWWRGEVGGMKGLIPHKYISVVEGMERKDRGKKEDSRGGSTGNLAEEQQQQSEHSTRMRVNSDSASLPGRQRTGSGSSGSIGGGNSGNMSPVRKLTLQVPEGRLMLPPQTTAVARQFSGHQERRHTLDSLRPVGAPDRQTVHIDKEVSRQMNSVFKELLSRQPPQEALGSSASPSSPPASVPSIPSVSSAAARPPVKKGGFGLRSRGLFKPQDQQD

Target ORF information:

RefSeq Version	XM_009296754.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 4b (arhgap4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296754.1

ORF Insert Sequence:

ATGACTTCAC ATGGAAAACT GAGAAAGGAA AAGGGGAGTC TGGCGGAGTA TGAGACTCAA 
ATCAAAGAGG TGCGTGGTCA GCTCTCAGAG CAGCTTAAGA TCCTAGACGC GCAGCTGGAG 
GTCAAGACAC AACAGCTGCA GGATATGAGC GAGTACCTGC GCAGACGAGG AGAGATTGAG 
GCTGAATATG CCCGATCTCT GGAGAAACTG AGTGAGAAGT TCACAGTGAA GACCAAGAGA 
AAGGAGCAGT TGGACCTGTC GGTGGCTCAG TGCTGGTCTG TTCTTCTGAC CCAAACCAGG 
CAGGAGAGCC GAGATCACAG TTCACTCAGT GAGCTCTGTG GCAACACACT CACGCAGCGC 
CTCTCACACT GCATAGAGGA CACACACCGC CTGGCAAAGC GGAGCAAGGA GGTTGGACTC 
CAGATGCAGG ATGAGCTTTT GAAAATCACC ACAGAGCTGC AGACGGCTTT GAAAACTTAT 
CATCAATACC ACATCGACTG TCTGGCTGCA GAAGGCAAGC TCAAAGAAGC CACGCGACTA 
GAAGAGAAGC AGACGGGCAA GTCAGCTGAT CTCGGCCTCA GCCAATCAGG AGGGCAAAGA 
CGCAGTTCTG TCAAAAAAAT GGAGAGACTG ATGGAGAAGA GACAAGGCAA AGTACAGGAA 
ACCCAGCTGA AGTGCACTAA AGCTCGCAAC GACTACTTGC TCAACTTGGC AGCAGCTAAT 
GCCTCCATGA ATAAATACTA TCTGCAGGAC ATTTGCACGC TGATTGATTG CACAGATCTG 
GGGTACCACC TGTCTGTGAC GCGGGTTATA CGCGGATACC TCTCATACAA AAACCGAATC 
GTCCAGAATA TGAAGAATGG CCTTCAGCAG CTGGACACTG CGGTGACTGG ACTCAACCAG 
GGTCAGGATA GAGACTCTCT GCTGCAGGCC CACAACATCG CTTTCTGCCT GCCCTTCCGC 
TTTCAGTACC AGCCCCATGA AGGAGATCAG GTGTGTGAGG TGAGCGCTGA AGGCCAGGTG 
AGATATGAGC TCGATACCAG GTTCCAGCAG CTGCAGTCCA GACTGGCATC TGTTACCCTC 
GAAACTGATG AGGTCAGCAA GACCCTTAAA GCAACCCACT CAAACCTGCT GGAGAGCGTC 
TGTGATGACG ACTGCAACCC CGCTCCAGAT GCCTCCAGTA CCCCGTCAGT GGAGGGCACT 
GCGGACGGCA CAGGAACAAA GCCCAGTCTC GCCAAACGTA GAGCCAATCA GCAGGACACC 
GAGACTTTCT ACTTCACGAA AGTGAAGGAG CATGTAAGTG GCAGCTCTCT GATCTCGAAA 
CTCCAAGCCA AGCATGACCT ACTTAAAGAG GCCATACAGA AAGCTGAGGC CATTGATAGC 
GACCCCTCCA GAATGCAGCC TGGTAGGGCA GTGCGTGTGC GGAAAGCCAG GCCCTGCTCC 
CAGTACAACC ACAAACTCTT CTCTGGAGAC ATGCTCTCCT TCATTCAGAG CTCAGGGCAG 
CAGATCCCCC TGGTGGTTGA AAGCTGCATT CGCTTCATCA ACCTGCATGG TCTTCATCAT 
GAAGGCATTT TTAGGGTTCC TGGATCACAG ACTGAAGTTA ATCATATCAG GGATGCTTTT 
GAAAGAGGCG AAGACCCACT CACTGACAGC GAGAGTGACA TTGATTCAGT GGCGGGGGTC 
CTGAAGCTCT ATTTCAGAGG ACTGGAGAAA CCTCTGTTCC CTGAGGAGAG CTTTAGCCAG 
CTCATGGAGT GTGTCCAAAT GGAAAACATG ACCGAAAAAG TAGCGCAGAT AAAGTCAGTA 
GTGTCGTCCT ATCCCAGACC TGTCATCATA GTGATGCGGT ATCTCTTCGC CTTCCTTCAT 
CACGTCTCTC AGTACAGTGA TGAGAACATG ATGCAGCCGT ATAATCTGGC TGTCTGCTTC 
GGGCCCAGTC TGCTCCGAGG CGTAGAGATG GGTGGTGACG AGGTGACCCT GGCTCCTCAG 
ATCAATGAGC TGGTCAAAAC CATGATCCTT CATCATGAGA ACATCTTCCC AGGACCTTCT 
GAACTTCCAG GCCCCGTTTA TGAGAAGTGC ATGACTTTGG AGCAGGAGTA CTGTGAACCC 
ATCACAGAGG AGGGCGAAGG AGACCCTGAA CATCTTCCCA GTGAAGATGC CGAGCTGTCA 
TTCAAGCAGG GTGAGCACCT CCTACTGCAC AGCAAGGCCT CAGCTGATTG GTGGAGAGGG 
GAAGTGGGAG GAATGAAAGG CTTGATCCCC CATAAGTACA TCAGTGTGGT AGAAGGGATG 
GAGCGTAAGG ACCGGGGAAA GAAGGAGGAC AGTAGAGGGG GAAGCACAGG AAACCTAGCA 
GAGGAGCAGC AGCAACAGTC TGAACACAGC ACTCGTATGC GAGTGAACAG CGACAGCGCA 
TCTTTGCCCG GCCGGCAGAG GACCGGCAGT GGAAGCAGCG GCAGCATTGG TGGAGGAAAC 
AGTGGGAATA TGAGCCCTGT ACGAAAGCTC ACCCTGCAGG TGCCTGAGGG ACGGCTCATG 
CTTCCTCCTC AGACCACTGC AGTCGCACGC CAATTCTCAG GTCATCAGGA GCGCAGACAC 
ACTTTAGACT CTCTGAGGCC AGTAGGAGCT CCAGACAGAC AGACAGTACA TATCGACAAG 
GAGGTCAGTC GTCAGATGAA CTCCGTGTTT AAGGAGCTGT TGTCTCGTCA GCCTCCTCAA 
GAGGCCTTGG GTTCGAGCGC TTCTCCATCC TCTCCTCCTG CTTCTGTCCC ATCCATCCCA 
TCGGTCTCCT CTGCTGCTGC TCGACCTCCG GTGAAGAAGG GAGGCTTTGG CCTGCGCAGC 
AGAGGCCTCT TCAAACCCCA GGACCAACAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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arhgap4b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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