ARHGAP4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGAP4
Entrez Gene ID	100124430
Full Name	Rho GTPase activating protein 4
General protein information	Preferred Names Rho GTPase activating protein 4 Names rho GTPase-activating protein 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005227657.3	XP_005227714.1	rho GTPase-activating protein 4 isoform X2
NM_001102540.2	NP_001096010.1	rho GTPase-activating protein 4
XM_005227656.3	XP_005227713.1	rho GTPase-activating protein 4 isoform X1
XM_005227656.4	XP_005227713.2	rho GTPase-activating protein 4 isoform X1
XM_005227657.4	XP_005227714.2	rho GTPase-activating protein 4 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARHGAP4	XP_549365.3
	ARHGAP4	XP_002806496.1
Danio rerio (zebrafish)	arhgap4b	XP_689729.3
Danio rerio (zebrafish)	arhgap4a	NP_956738.1
Homo sapiens (human)	ARHGAP4	NP_001158213.1
Pan troglodytes (chimpanzee)	ARHGAP4	XP_003317822.1
Mus musculus (house mouse)	Arhgap4	NP_619536.2
Xenopus tropicalis (tropical clawed frog)	arhgap4	NP_001123805.1
Bos taurus (cattle)	ARHGAP4	NP_001096010.1
Rattus norvegicus (Norway rat)	Arhgap4	NP_653341.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ARHGAP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGAP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40982	NM_001102540.2 Latest version!	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa35258	XM_005227656.3 Latest version!	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa35259	XM_005227657.3 Latest version!	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa238219	XM_005227656.4 Latest version!	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa238220	XM_005227657.4 Latest version!	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGAP4 ( NM_001102540.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096010.1 Bos taurus Rho GTPase activating protein 4 (ARHGAP4), mRNA.

Price & Availability↑

CloneID	OBa40982
Clone ID Related Accession (Same CDS sequence)	NM_001102540.2
Accession Version	NM_001102540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151274.1. On Aug 31, 2012 this sequence version replaced NM_001102540.1. ##Evidence-Data-START## Transcript exon combination :: BC151274.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCCACGC ATGGGAAACT ACGCCGAGAG CGAGGGCCGC AGGCTGACTA TGAGGCGCAG 
GTCAAAGAGA TGCGCTGGCA ACTGGGTGAG CAACTGCGCT GCCTGGAGCT GCAGGGCGAA 
CTGCGACGGG AGCTGCTGCA GGAGCTGGCT GAGTTCATGC GGCGCCGCGC CGAGGTTGAG 
CTTGAGTACT CCCGGGGCCT CGACAAGCTG GTAGAGCGAT TCTCCGGCCG TGGAGGCCGC 
CTCGGGGGCA GCAGTCGGGA GCACCAGAGC TTCCGGAAGG AGCCGTCCCT GCTGTCGCCC 
TTGCACTGCT GGGCTGTGTT GTTGCAGCAG ACTCGGCAGC AGAGCCGGGA AAGCACAGCT 
CTCAGCGAGG TGCTGGGCGG GCCCCTGGCC CAGCGCCTGA GCTACATTGC CGAGGATGTG 
GGGCGCCTGG TCAAGAAGAG TAAGGATCTG GAGCAACAGC TGCAAGAGGA GCTCCTGGAG 
GTGGTGTCAG AGCTTCAGAT GGCCAAGAAG ACCTATCAGG TCTACCACAC GGAGAGCCTG 
AGTGCTGAGG CCAAACTCCG TGAAGCCGAG CGGCAGGAGG AGAAGCGGGC AGGCCGAAGT 
GCCACTGCCA CCACCACCAG CACCGAGTCT GGGCCCCTCC GCAAAGGCTC TGTCAAGAAG 
GGAGGGCGGC TGGTGGAGAA GCGTCACGCC AAGTTCTTGG AGCACAAACT CAAGTGTACA 
AAGGCTCGCA ATGAGTACCT GCTAAGCCTG GCCAGCGTCA ACGCCGCCGT CAGCAACTAC 
TACCTGCGAG ACGTCATGGA CCTGATGGAT TGTTGTGACA CAGGATTCCA CCTGGCCCTG 
GGCCAAGTGC TCCGGAGCTA CACGGCTGCG GAGAGCCGCA TCCAAGCCTC CCAGATGCAG 
GGCCTGGGCA GCCTGGAGGA GGCCGTGGAT GCCCTGGATC CCATAGGTGA CAAGGCCAAG 
GTGCTGGAGG TGCACGCTAT CGCCTTCTGT CCCCCGCTGC GTTTCGACTA CCAGCCCCAC 
GAGGGGGATG AGGTTGCTGA GATCCAGGTT GAGATGGAGC TGCGGGATGA GATTCTACCC 
AGAGCCCAGA ATATCCAGAG CCGCCTGGAC CGCCAGACCA TCGAGACAGA GGAGGTGAAT 
AAGACGCTGA AGGCCACACT GCAGGCCCTG CTGGAGGTGG TGGCGTCAGA AGACAGTGAC 
ATGCTCGACT CCTTCCAGGC CAGCCTGTCC ACCGAGTCCC TCAAGTCCAC CAGCTCGGAC 
CCGGGGGCTC GGCAGGCTGG CCGGCGGCGG GGCCAGCAGC AGGAGACTGA GACCTTCTAC 
ATCACGAAAC TCCAGGAGTA CCTGAGTGGA CGGAGTATCC TTGCCAAGCT GCAGGCCAAG 
CATGAGAAAC TGCAGGACGC CATCCAGCGA GGTGACAAGG AAGACCGGGA GGTGACTTGG 
ACCCAGTACA CACAGAGAAA ACTCCAGAAG AGCCGTGCCC CCCGCCCCAG CTCCCAGTAT 
AACCAGAAAC TCTTCGGGGG AGACATGGAG AAGTTTATCC AGAGCTCAGG CCAGCCCGTG 
CCCCTGGTGG TGGAGAGCTG TGTCCGCTTC ATTAACCTCA ATGGCCTGCA GCATGAGGGC 
ATCTTCCGGG TGTCGGGCGC CCAGCCCCAG ATCTCGGAGA TCCGTGATGC TTTTGAGAGA 
GGAGAGGACC CGCTGGTGGA GGGCTGCACT GCCCATGACC TGGACTCGGT GGCGGGCGTG 
CTGAAACTCT ACTTCCGGTG CTTGCAGCCC CCCATATTCC CACCAGACCT GTTTGGAGAG 
CTGCTGGCTT CTGCAGAGCT GGAGGCTGTG GCCGAGCGGG TGGAGCACGT GGCTGGCCTC 
CTGGCCCGGC TGCCCGGACC CGTGCTGGTG GTCTTGCGGT ACCTCTTCAC CTTCCTCAAC 
CACCTGGCCC AGTACAGTGA TGAGAACATG ATGGACCCCT ACAACCTGGC CGTGTGCTTT 
GGGCCGACGC TGCTGCCCGT CCCTGCTGGG CAGGACCCAG TGGCTTTGCA AGGCCGTGTC 
AACCAGCTGG TGCAGACCCT CATCATCCAG CCTGGACGGG TCTTCCCGGC CCCGGCCCAG 
CTGCCCGGCC CCGTCTATGA GAAGTGCATG GCACCACCTT CTGCCAGCTG CTTGGGGGAT 
GCCCAGTTGG AGGGCCCGAC GGGGGACAAC GAGTTGGAGC TGGAGCCGGG GACCTCAGCC 
CAGGAGGAGG ACCAGGAGGG TGTCGTGGAG GCCGTGGCTT GCTTTGCATA CACAGGCCGC 
ACGGCCCAGG AGCTGACCTT CCAGCGCGGG GACATCTTGC GGCTGCACGA GCGGGCCTCA 
GGCGACTGGT GGCGGGGCGA GCACGCGGGG GCACGGGGGC TCATCCCCCA CAAGTATATC 
ACTCTGCCTG CGGGAGCAGA GAAGCTCGCG GCGGGCCAGG GGCTGCTGGC CGCCGGGGAC 
CTGCTGAGCA GCTCAGAGGG GCTCCCCATG GTGGAGCTCG TCCAGAGGCC AGAGCCGTGC 
ACCCCACCCG AGATCCCTCT TGGTATCCCC GGGAGTCACT CCGGGTACCG ACGGCCCTGC 
GTGGTCCCAG CATCCCCAGA ACGACACGTG GAGGTGGATA AGGCTGTGGC ACAGACCATG 
GACTCTGTGT TTAAGGAGCT CTTGGGGAAG ACTGCCCTCC GTCAGGGCCT CGGACCGGGC 
ACCAACAACT CTTCGAGCCC CGGGCCTCGC AGCCCGAAGC CTCCAGGCTG TGGGCGCCTG 
GGCAAGAACA AAGGCTTCTC ACGGGGCCCT GGTGCCCCAG CCTCACCCTC GACCTCCCAT 
CCCCAGGGCC TAGACTCACC CTTCAAGTCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096010.1
CDS	65..2920
Translation	MATHGKLRRERGPQADYEAQVKEMRWQLGEQLRCLELQGELRRELLQELAEFMRRRAEVELEYSRGLDKLVERFSGRGGRLGGSSREHQSFRKEPSLLSPLHCWAVLLQQTRQQSRESTALSEVLGGPLAQRLSYIAEDVGRLVKKSKDLEQQLQEELLEVVSELQMAKKTYQVYHTESLSAEAKLREAERQEEKRAGRSATATTTSTESGPLRKGSVKKGGRLVEKRHAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLRDVMDLMDCCDTGFHLALGQVLRSYTAAESRIQASQMQGLGSLEEAVDALDPIGDKAKVLEVHAIAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDRQTIETEEVNKTLKATLQALLEVVASEDSDMLDSFQASLSTESLKSTSSDPGARQAGRRRGQQQETETFYITKLQEYLSGRSILAKLQAKHEKLQDAIQRGDKEDREVTWTQYTQRKLQKSRAPRPSSQYNQKLFGGDMEKFIQSSGQPVPLVVESCVRFINLNGLQHEGIFRVSGAQPQISEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRCLQPPIFPPDLFGELLASAELEAVAERVEHVAGLLARLPGPVLVVLRYLFTFLNHLAQYSDENMMDPYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLIIQPGRVFPAPAQLPGPVYEKCMAPPSASCLGDAQLEGPTGDNELELEPGTSAQEEDQEGVVEAVACFAYTGRTAQELTFQRGDILRLHERASGDWWRGEHAGARGLIPHKYITLPAGAEKLAAGQGLLAAGDLLSSSEGLPMVELVQRPEPCTPPEIPLGIPGSHSGYRRPCVVPASPERHVEVDKAVAQTMDSVFKELLGKTALRQGLGPGTNNSSSPGPRSPKPPGCGRLGKNKGFSRGPGAPASPSTSHPQGLDSPFKSH

Target ORF information:

RefSeq Version	NM_001102540.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102540.2

ORF Insert Sequence:

ATGGCCACGC ATGGGAAACT ACGCCGAGAG CGAGGGCCGC AGGCTGACTA TGAGGCGCAG 
GTCAAAGAGA TGCGCTGGCA ACTGGGTGAG CAACTGCGCT GCCTGGAGCT GCAGGGCGAA 
CTGCGACGGG AGCTGCTGCA GGAGCTGGCT GAGTTCATGC GGCGCCGCGC CGAGGTTGAG 
CTTGAGTACT CCCGGGGCCT CGACAAGCTG GTAGAGCGAT TCTCCGGCCG TGGAGGCCGC 
CTCGGGGGCA GCAGTCGGGA GCACCAGAGC TTCCGGAAGG AGCCGTCCCT GCTGTCGCCC 
TTGCACTGCT GGGCTGTGTT GTTGCAGCAG ACTCGGCAGC AGAGCCGGGA AAGCACAGCT 
CTCAGCGAGG TGCTGGGCGG GCCCCTGGCC CAGCGCCTGA GCTACATTGC CGAGGATGTG 
GGGCGCCTGG TCAAGAAGAG TAAGGATCTG GAGCAACAGC TGCAAGAGGA GCTCCTGGAG 
GTGGTGTCAG AGCTTCAGAT GGCCAAGAAG ACCTATCAGG TCTACCACAC GGAGAGCCTG 
AGTGCTGAGG CCAAACTCCG TGAAGCCGAG CGGCAGGAGG AGAAGCGGGC AGGCCGAAGT 
GCCACTGCCA CCACCACCAG CACCGAGTCT GGGCCCCTCC GCAAAGGCTC TGTCAAGAAG 
GGAGGGCGGC TGGTGGAGAA GCGTCACGCC AAGTTCTTGG AGCACAAACT CAAGTGTACA 
AAGGCTCGCA ATGAGTACCT GCTAAGCCTG GCCAGCGTCA ACGCCGCCGT CAGCAACTAC 
TACCTGCGAG ACGTCATGGA CCTGATGGAT TGTTGTGACA CAGGATTCCA CCTGGCCCTG 
GGCCAAGTGC TCCGGAGCTA CACGGCTGCG GAGAGCCGCA TCCAAGCCTC CCAGATGCAG 
GGCCTGGGCA GCCTGGAGGA GGCCGTGGAT GCCCTGGATC CCATAGGTGA CAAGGCCAAG 
GTGCTGGAGG TGCACGCTAT CGCCTTCTGT CCCCCGCTGC GTTTCGACTA CCAGCCCCAC 
GAGGGGGATG AGGTTGCTGA GATCCAGGTT GAGATGGAGC TGCGGGATGA GATTCTACCC 
AGAGCCCAGA ATATCCAGAG CCGCCTGGAC CGCCAGACCA TCGAGACAGA GGAGGTGAAT 
AAGACGCTGA AGGCCACACT GCAGGCCCTG CTGGAGGTGG TGGCGTCAGA AGACAGTGAC 
ATGCTCGACT CCTTCCAGGC CAGCCTGTCC ACCGAGTCCC TCAAGTCCAC CAGCTCGGAC 
CCGGGGGCTC GGCAGGCTGG CCGGCGGCGG GGCCAGCAGC AGGAGACTGA GACCTTCTAC 
ATCACGAAAC TCCAGGAGTA CCTGAGTGGA CGGAGTATCC TTGCCAAGCT GCAGGCCAAG 
CATGAGAAAC TGCAGGACGC CATCCAGCGA GGTGACAAGG AAGACCGGGA GGTGACTTGG 
ACCCAGTACA CACAGAGAAA ACTCCAGAAG AGCCGTGCCC CCCGCCCCAG CTCCCAGTAT 
AACCAGAAAC TCTTCGGGGG AGACATGGAG AAGTTTATCC AGAGCTCAGG CCAGCCCGTG 
CCCCTGGTGG TGGAGAGCTG TGTCCGCTTC ATTAACCTCA ATGGCCTGCA GCATGAGGGC 
ATCTTCCGGG TGTCGGGCGC CCAGCCCCAG ATCTCGGAGA TCCGTGATGC TTTTGAGAGA 
GGAGAGGACC CGCTGGTGGA GGGCTGCACT GCCCATGACC TGGACTCGGT GGCGGGCGTG 
CTGAAACTCT ACTTCCGGTG CTTGCAGCCC CCCATATTCC CACCAGACCT GTTTGGAGAG 
CTGCTGGCTT CTGCAGAGCT GGAGGCTGTG GCCGAGCGGG TGGAGCACGT GGCTGGCCTC 
CTGGCCCGGC TGCCCGGACC CGTGCTGGTG GTCTTGCGGT ACCTCTTCAC CTTCCTCAAC 
CACCTGGCCC AGTACAGTGA TGAGAACATG ATGGACCCCT ACAACCTGGC CGTGTGCTTT 
GGGCCGACGC TGCTGCCCGT CCCTGCTGGG CAGGACCCAG TGGCTTTGCA AGGCCGTGTC 
AACCAGCTGG TGCAGACCCT CATCATCCAG CCTGGACGGG TCTTCCCGGC CCCGGCCCAG 
CTGCCCGGCC CCGTCTATGA GAAGTGCATG GCACCACCTT CTGCCAGCTG CTTGGGGGAT 
GCCCAGTTGG AGGGCCCGAC GGGGGACAAC GAGTTGGAGC TGGAGCCGGG GACCTCAGCC 
CAGGAGGAGG ACCAGGAGGG TGTCGTGGAG GCCGTGGCTT GCTTTGCATA CACAGGCCGC 
ACGGCCCAGG AGCTGACCTT CCAGCGCGGG GACATCTTGC GGCTGCACGA GCGGGCCTCA 
GGCGACTGGT GGCGGGGCGA GCACGCGGGG GCACGGGGGC TCATCCCCCA CAAGTATATC 
ACTCTGCCTG CGGGAGCAGA GAAGCTCGCG GCGGGCCAGG GGCTGCTGGC CGCCGGGGAC 
CTGCTGAGCA GCTCAGAGGG GCTCCCCATG GTGGAGCTCG TCCAGAGGCC AGAGCCGTGC 
ACCCCACCCG AGATCCCTCT TGGTATCCCC GGGAGTCACT CCGGGTACCG ACGGCCCTGC 
GTGGTCCCAG CATCCCCAGA ACGACACGTG GAGGTGGATA AGGCTGTGGC ACAGACCATG 
GACTCTGTGT TTAAGGAGCT CTTGGGGAAG ACTGCCCTCC GTCAGGGCCT CGGACCGGGC 
ACCAACAACT CTTCGAGCCC CGGGCCTCGC AGCCCGAAGC CTCCAGGCTG TGGGCGCCTG 
GGCAAGAACA AAGGCTTCTC ACGGGGCCCT GGTGCCCCAG CCTCACCCTC GACCTCCCAT 
CCCCAGGGCC TAGACTCACC CTTCAAGTCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP4 ( XM_005227656.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227713.1 Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35258
Clone ID Related Accession (Same CDS sequence)	XM_005227656.3
Accession Version	XM_005227656.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTGGC AACTGGGTGA GCAACTGCGC TGCCTGGAGC TGCAGGGCGA ACTGCGACGG 
GAGCTGCTGC AGGAGCTGGC TGAGTTCATG CGGCGCCGCG CCGAGGTTGA GCTTGAGTAC 
TCCCGGGGCC TCGACAAGCT GGTAGAGCGA TTCTCCGGCC GTGGAGGCCG CCTCGGGGGC 
AGCAGTCGGG AGCACCAGAG CTTCCGGAAG GAGCCGTCCC TGCTGTCGCC CTTGCACTGC 
TGGGCTGTGT TGTTGCAGCA GACTCGGCAG CAGAGCCGGG AAAGCACAGC TCTCAGCGAG 
GTGCTGGGCG GGCCCCTGGC CCAGCGCCTG AGCTACATTG CCGAGGATGT GGGGCGCCTG 
GTCAAGAAGA GTAAGGATCT GGAGCAACAG CTGCAAGAGG AGCTCCTGGA GGTGGTGTCA 
GAGCTTCAGA TGGCCAAGAA GACCTATCAG GTCTACCACA CGGAGAGCCT GAGTGCTGAG 
GCCAAACTCC GTGAAGCCGA GCGGCAGGAG GAGAAGCGGG CAGGCCGAAG TGCCACTGCC 
ACCACCACCA GCACCGAGTC TGGGCCCCTC CGCAAAGGCT CTGTCAAGAA GGGAGGGCGG 
CTGGTGGAGA AGCGTCACGC CAAGTTCTTG GAGCACAAAC TCAAGTGTAC AAAGGCTCGC 
AATGAGTACC TGCTAAGCCT GGCCAGCGTC AACGCCGCCG TCAGCAACTA CTACCTGCGA 
GACGTCATGG ACCTGATGGA TTGTTGTGAC ACAGGATTCC ACCTGGCCCT GGGCCAAGTG 
CTCCGGAGCT ACACGGCTGC GGAGAGCCGC ATCCAAGCCT CCCAGATGCA GGGCCTGGGC 
AGCCTGGAGG AGGCCGTGGA TGCCCTGGAT CCCATAGGTG ACAAGGCCAA GGTGCTGGAG 
GTGCACGCTA TCGCCTTCTG TCCCCCGCTG CGTTTCGACT ACCAGCCCCA CGAGGGGGAT 
GAGGTTGCTG AGATCCAGGT TGAGATGGAG CTGCGGGATG AGATTCTACC CAGAGCCCAG 
AATATCCAGA GCCGCCTGGA CCGCCAGACC ATCGAGACAG AGGAGGTGAA TAAGACGCTG 
AAGGCCACAC TGCAGGCCCT GCTGGAGGTG GTGGCGTCAG AAGACAGTGA CATGCTCGAC 
TCCTTCCAGG CCAGCCTGTC CACCGAGTCC CTCAAGTCCA CCAGCTCGGA CCCGGGGGCT 
CGGCAGGCTG GCCGGCGGCG GGGCCAGCAG CAGGAGACTG AGACCTTCTA CATCACGAAA 
CTCCAGGAGT ACGTGAGTGG ACGGAGTATC CTTGCCAAGC TGCAGGCCAA GCATGAGAAA 
CTGCAGGACG CCATCCAGCG AGGTGACAAG GAAGACCGGG AGGTGACTTG GACCCAGTAC 
ACACAGAGAA AACTCCAGAA GAGCCGTGCC CCCCGCCCCA GCTCCCAGTA TAACCAGAAA 
CTCTTCGGGG GAGACATGGA GAAGTTTATC CAGAGCTCAG GCCAGCCCGT GCCCCTGGTG 
GTGGAGAGCT GTGTCCGCTT CATTAACCTC AATGGCCTGC AGCATGAGGG CATCTTCCGG 
GTGTCGGGCG CCCAGCCCCA GATCTCGGAG ATCCGTGATG CTTTTGAGAG AGGAGAGGAC 
CCGCTGGTGG AGGGCTGCAC TGCCCATGAC CTGGACTCGG TGGCGGGCGT GCTGAAACTC 
TACTTCCGGA GCTTGCAGCC CCCCATATTC CCACCAGACC TGTTTGGAGA GCTGCTGGCT 
TCTGCAGAGC TGGAGGCTGT GGCCGAGCGG GTGGAGCACG TGGCTGGCCT CCTGGCCCGG 
CTGCCCGGAC CCGTGCTGGT GGTCTTGCGG TACCTCTTCA CCTTCCTCAA CCACCTGGCC 
CAGTACAGTG ATGAGAACAT GATGGACCCC TACAACCTGG CCGTGTGCTT TGGGCCGACG 
CTGCTGCCCG TCCCTGCTGG GCAGGACCCA GTGGCTTTGC AAGGCCGTGT CAACCAGCTG 
GTGCAGACCC TCATCATCCA GCCTGGACGG GTCTTCCCGG CCCCGGCCCA GCTGCCCGGC 
CCCGTCTATG AGAAGTGCAT GGCACCACCT TCTGCCAGCT GCTTGGGGGA TGCCCAGTTG 
GAGGGCCCGA CGGGGGACAA CGAGTTGGAG CTGGAGCCGG GGACCTCAGC CCAGGAGGAG 
GACCAGGAGG GTGTCGTGGA GGCCGTGGCT TGCTTTGCAT ACACAGGCCG CACGGCCCAG 
GAGCTGACCT TCCAGCGCGG GGACATCTTG CGGCTGCACG AGCGGGCCTC AGGCGACTGG 
TGGCGGGGCG AGCACGCGGG GGCACGGGGG CTCATCCCCC ACAAGTATAT CACTCTGCCT 
GCGGGAGCAG AGAAGCTCGC GGCGGGCCAG GGGCTGCTGG CCGCCGGGGA CCTGCTGAGC 
AGCTCAGAGG GGCTCCCCAT GGTGGAGCTC GTCCAGAGGC CAGAGCCGTG CACCCCACCC 
GAGATCCCTC TTGGTATCCC CGGGAGTCAC TCCGGGTACC GACGGCCCTG CGTGGTCCCA 
GCATCCCCAG AACGACACGT GGAGGTGGAT AAGGCTGTGG CACAGACCAT GGACTCTGTG 
TTTAAGGAGC TCTTGGGGAA GACTGCCCTC CGTCAGGGCC TCGGACCGGG CACCAACAAC 
TCTTCGAGCC CCGGGCCTCG CAGCCCGAAG CCTCCAGGCT GTGGGCGCCT GGGCAAGAAC 
AAAGGCTTCT CACGGGGCCC TGGTGCCCCA GCCTCACCCT CGACCTCCCA TCCCCAGGGC 
CTAGACTCAC CCTTCAAGTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227713.1
CDS	170..2956
Translation	MRWQLGEQLRCLELQGELRRELLQELAEFMRRRAEVELEYSRGLDKLVERFSGRGGRLGGSSREHQSFRKEPSLLSPLHCWAVLLQQTRQQSRESTALSEVLGGPLAQRLSYIAEDVGRLVKKSKDLEQQLQEELLEVVSELQMAKKTYQVYHTESLSAEAKLREAERQEEKRAGRSATATTTSTESGPLRKGSVKKGGRLVEKRHAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLRDVMDLMDCCDTGFHLALGQVLRSYTAAESRIQASQMQGLGSLEEAVDALDPIGDKAKVLEVHAIAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDRQTIETEEVNKTLKATLQALLEVVASEDSDMLDSFQASLSTESLKSTSSDPGARQAGRRRGQQQETETFYITKLQEYVSGRSILAKLQAKHEKLQDAIQRGDKEDREVTWTQYTQRKLQKSRAPRPSSQYNQKLFGGDMEKFIQSSGQPVPLVVESCVRFINLNGLQHEGIFRVSGAQPQISEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLQPPIFPPDLFGELLASAELEAVAERVEHVAGLLARLPGPVLVVLRYLFTFLNHLAQYSDENMMDPYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLIIQPGRVFPAPAQLPGPVYEKCMAPPSASCLGDAQLEGPTGDNELELEPGTSAQEEDQEGVVEAVACFAYTGRTAQELTFQRGDILRLHERASGDWWRGEHAGARGLIPHKYITLPAGAEKLAAGQGLLAAGDLLSSSEGLPMVELVQRPEPCTPPEIPLGIPGSHSGYRRPCVVPASPERHVEVDKAVAQTMDSVFKELLGKTALRQGLGPGTNNSSSPGPRSPKPPGCGRLGKNKGFSRGPGAPASPSTSHPQGLDSPFKSH

Target ORF information:

RefSeq Version	XM_005227656.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227656.3

ORF Insert Sequence:

ATGCGCTGGC AACTGGGTGA GCAACTGCGC TGCCTGGAGC TGCAGGGCGA ACTGCGACGG 
GAGCTGCTGC AGGAGCTGGC TGAGTTCATG CGGCGCCGCG CCGAGGTTGA GCTTGAGTAC 
TCCCGGGGCC TCGACAAGCT GGTAGAGCGA TTCTCCGGCC GTGGAGGCCG CCTCGGGGGC 
AGCAGTCGGG AGCACCAGAG CTTCCGGAAG GAGCCGTCCC TGCTGTCGCC CTTGCACTGC 
TGGGCTGTGT TGTTGCAGCA GACTCGGCAG CAGAGCCGGG AAAGCACAGC TCTCAGCGAG 
GTGCTGGGCG GGCCCCTGGC CCAGCGCCTG AGCTACATTG CCGAGGATGT GGGGCGCCTG 
GTCAAGAAGA GTAAGGATCT GGAGCAACAG CTGCAAGAGG AGCTCCTGGA GGTGGTGTCA 
GAGCTTCAGA TGGCCAAGAA GACCTATCAG GTCTACCACA CGGAGAGCCT GAGTGCTGAG 
GCCAAACTCC GTGAAGCCGA GCGGCAGGAG GAGAAGCGGG CAGGCCGAAG TGCCACTGCC 
ACCACCACCA GCACCGAGTC TGGGCCCCTC CGCAAAGGCT CTGTCAAGAA GGGAGGGCGG 
CTGGTGGAGA AGCGTCACGC CAAGTTCTTG GAGCACAAAC TCAAGTGTAC AAAGGCTCGC 
AATGAGTACC TGCTAAGCCT GGCCAGCGTC AACGCCGCCG TCAGCAACTA CTACCTGCGA 
GACGTCATGG ACCTGATGGA TTGTTGTGAC ACAGGATTCC ACCTGGCCCT GGGCCAAGTG 
CTCCGGAGCT ACACGGCTGC GGAGAGCCGC ATCCAAGCCT CCCAGATGCA GGGCCTGGGC 
AGCCTGGAGG AGGCCGTGGA TGCCCTGGAT CCCATAGGTG ACAAGGCCAA GGTGCTGGAG 
GTGCACGCTA TCGCCTTCTG TCCCCCGCTG CGTTTCGACT ACCAGCCCCA CGAGGGGGAT 
GAGGTTGCTG AGATCCAGGT TGAGATGGAG CTGCGGGATG AGATTCTACC CAGAGCCCAG 
AATATCCAGA GCCGCCTGGA CCGCCAGACC ATCGAGACAG AGGAGGTGAA TAAGACGCTG 
AAGGCCACAC TGCAGGCCCT GCTGGAGGTG GTGGCGTCAG AAGACAGTGA CATGCTCGAC 
TCCTTCCAGG CCAGCCTGTC CACCGAGTCC CTCAAGTCCA CCAGCTCGGA CCCGGGGGCT 
CGGCAGGCTG GCCGGCGGCG GGGCCAGCAG CAGGAGACTG AGACCTTCTA CATCACGAAA 
CTCCAGGAGT ACGTGAGTGG ACGGAGTATC CTTGCCAAGC TGCAGGCCAA GCATGAGAAA 
CTGCAGGACG CCATCCAGCG AGGTGACAAG GAAGACCGGG AGGTGACTTG GACCCAGTAC 
ACACAGAGAA AACTCCAGAA GAGCCGTGCC CCCCGCCCCA GCTCCCAGTA TAACCAGAAA 
CTCTTCGGGG GAGACATGGA GAAGTTTATC CAGAGCTCAG GCCAGCCCGT GCCCCTGGTG 
GTGGAGAGCT GTGTCCGCTT CATTAACCTC AATGGCCTGC AGCATGAGGG CATCTTCCGG 
GTGTCGGGCG CCCAGCCCCA GATCTCGGAG ATCCGTGATG CTTTTGAGAG AGGAGAGGAC 
CCGCTGGTGG AGGGCTGCAC TGCCCATGAC CTGGACTCGG TGGCGGGCGT GCTGAAACTC 
TACTTCCGGA GCTTGCAGCC CCCCATATTC CCACCAGACC TGTTTGGAGA GCTGCTGGCT 
TCTGCAGAGC TGGAGGCTGT GGCCGAGCGG GTGGAGCACG TGGCTGGCCT CCTGGCCCGG 
CTGCCCGGAC CCGTGCTGGT GGTCTTGCGG TACCTCTTCA CCTTCCTCAA CCACCTGGCC 
CAGTACAGTG ATGAGAACAT GATGGACCCC TACAACCTGG CCGTGTGCTT TGGGCCGACG 
CTGCTGCCCG TCCCTGCTGG GCAGGACCCA GTGGCTTTGC AAGGCCGTGT CAACCAGCTG 
GTGCAGACCC TCATCATCCA GCCTGGACGG GTCTTCCCGG CCCCGGCCCA GCTGCCCGGC 
CCCGTCTATG AGAAGTGCAT GGCACCACCT TCTGCCAGCT GCTTGGGGGA TGCCCAGTTG 
GAGGGCCCGA CGGGGGACAA CGAGTTGGAG CTGGAGCCGG GGACCTCAGC CCAGGAGGAG 
GACCAGGAGG GTGTCGTGGA GGCCGTGGCT TGCTTTGCAT ACACAGGCCG CACGGCCCAG 
GAGCTGACCT TCCAGCGCGG GGACATCTTG CGGCTGCACG AGCGGGCCTC AGGCGACTGG 
TGGCGGGGCG AGCACGCGGG GGCACGGGGG CTCATCCCCC ACAAGTATAT CACTCTGCCT 
GCGGGAGCAG AGAAGCTCGC GGCGGGCCAG GGGCTGCTGG CCGCCGGGGA CCTGCTGAGC 
AGCTCAGAGG GGCTCCCCAT GGTGGAGCTC GTCCAGAGGC CAGAGCCGTG CACCCCACCC 
GAGATCCCTC TTGGTATCCC CGGGAGTCAC TCCGGGTACC GACGGCCCTG CGTGGTCCCA 
GCATCCCCAG AACGACACGT GGAGGTGGAT AAGGCTGTGG CACAGACCAT GGACTCTGTG 
TTTAAGGAGC TCTTGGGGAA GACTGCCCTC CGTCAGGGCC TCGGACCGGG CACCAACAAC 
TCTTCGAGCC CCGGGCCTCG CAGCCCGAAG CCTCCAGGCT GTGGGCGCCT GGGCAAGAAC 
AAAGGCTTCT CACGGGGCCC TGGTGCCCCA GCCTCACCCT CGACCTCCCA TCCCCAGGGC 
CTAGACTCAC CCTTCAAGTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP4 ( XM_005227657.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227714.1 Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X2, mRNA.

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CloneID	OBa35259
Clone ID Related Accession (Same CDS sequence)	XM_005227657.3
Accession Version	XM_005227657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC ATGGGAAACT ACGCCGAGAG CGAGGGCCGC AGGCTGACTA TGAGGCGCAG 
GTCAAAGAGA TGCGCTGGCA ACTGGGTGAG CAACTGCGCT GCCTGGAGCT GCAGGGCGAA 
CTGCGACGGG AGCTGCTGCA GGAGCTGGCT GAGTTCATGC GGCGCCGCGC CGAGGTTGAG 
CTTGAGTACT CCCGGGGCCT CGACAAGCTG GTAGAGCGAT TCTCCGGCCG TGGAGGCCGC 
CTCGGGGGCA GCAGTCGGGA GCACCAGAGC TTCCGGAAGG AGCCGTCCCT GCTGTCGCCC 
TTGCACTGCT GGGCTGTGTT GTTGCAGCAG ACTCGGCAGC AGAGCCGGGA AAGCACAGCT 
CTCAGCGAGG TGCTGGGCGG GCCCCTGGCC CAGCGCCTGA GCTACATTGC CGAGGATGTG 
GGGCGCCTGG TCAAGAAGAG TAAGGATCTG GAGCAACAGC TGCAAGAGGA GCTCCTGGAG 
GTGGTGTCAG AGCTTCAGAT GCGTCACGCC AAGTTCTTGG AGCACAAACT CAAGTGTACA 
AAGGCTCGCA ATGAGTACCT GCTAAGCCTG GCCAGCGTCA ACGCCGCCGT CAGCAACTAC 
TACCTGCGAG ACGTCATGGA CCTGATGGAT TGTTGTGACA CAGGATTCCA CCTGGCCCTG 
GGCCAAGTGC TCCGGAGCTA CACGGCTGCG GAGAGCCGCA TCCAAGCCTC CCAGATGCAG 
GGCCTGGGCA GCCTGGAGGA GGCCGTGGAT GCCCTGGATC CCATAGGTGA CAAGGCCAAG 
GTGCTGGAGG TGCACGCTAT CGCCTTCTGT CCCCCGCTGC GTTTCGACTA CCAGCCCCAC 
GAGGGGGATG AGGTTGCTGA GATCCAGGTT GAGATGGAGC TGCGGGATGA GATTCTACCC 
AGAGCCCAGA ATATCCAGAG CCGCCTGGAC CGCCAGACCA TCGAGACAGA GGAGGTGAAT 
AAGACGCTGA AGGCCACACT GCAGGCCCTG CTGGAGGTGG TGGCGTCAGA AGACAGTGAC 
ATGCTCGACT CCTTCCAGGC CAGCCTGTCC ACCGAGTCCC TCAAGTCCAC CAGCTCGGAC 
CCGGGGGCTC GGCAGGCTGG CCGGCGGCGG GGCCAGCAGC AGGAGACTGA GACCTTCTAC 
ATCACGAAAC TCCAGGAGTA CGTGAGTGGA CGGAGTATCC TTGCCAAGCT GCAGGCCAAG 
CATGAGAAAC TGCAGGACGC CATCCAGCGA GGTGACAAGG AAGACCGGGA GGTGACTTGG 
ACCCAGTACA CACAGAGAAA ACTCCAGAAG AGCCGTGCCC CCCGCCCCAG CTCCCAGTAT 
AACCAGAAAC TCTTCGGGGG AGACATGGAG AAGTTTATCC AGAGCTCAGG CCAGCCCGTG 
CCCCTGGTGG TGGAGAGCTG TGTCCGCTTC ATTAACCTCA ATGGCCTGCA GCATGAGGGC 
ATCTTCCGGG TGTCGGGCGC CCAGCCCCAG ATCTCGGAGA TCCGTGATGC TTTTGAGAGA 
GGAGAGGACC CGCTGGTGGA GGGCTGCACT GCCCATGACC TGGACTCGGT GGCGGGCGTG 
CTGAAACTCT ACTTCCGGAG CTTGCAGCCC CCCATATTCC CACCAGACCT GTTTGGAGAG 
CTGCTGGCTT CTGCAGAGCT GGAGGCTGTG GCCGAGCGGG TGGAGCACGT GGCTGGCCTC 
CTGGCCCGGC TGCCCGGACC CGTGCTGGTG GTCTTGCGGT ACCTCTTCAC CTTCCTCAAC 
CACCTGGCCC AGTACAGTGA TGAGAACATG ATGGACCCCT ACAACCTGGC CGTGTGCTTT 
GGGCCGACGC TGCTGCCCGT CCCTGCTGGG CAGGACCCAG TGGCTTTGCA AGGCCGTGTC 
AACCAGCTGG TGCAGACCCT CATCATCCAG CCTGGACGGG TCTTCCCGGC CCCGGCCCAG 
CTGCCCGGCC CCGTCTATGA GAAGTGCATG GCACCACCTT CTGCCAGCTG CTTGGGGGAT 
GCCCAGTTGG AGGGCCCGAC GGGGGACAAC GAGTTGGAGC TGGAGCCGGG GACCTCAGCC 
CAGGAGGAGG ACCAGGAGGG TGTCGTGGAG GCCGTGGCTT GCTTTGCATA CACAGGCCGC 
ACGGCCCAGG AGCTGACCTT CCAGCGCGGG GACATCTTGC GGCTGCACGA GCGGGCCTCA 
GGCGACTGGT GGCGGGGCGA GCACGCGGGG GCACGGGGGC TCATCCCCCA CAAGTATATC 
ACTCTGCCTG CGGGAGCAGA GAAGCTCGCG GCGGGCCAGG GGCTGCTGGC CGCCGGGGAC 
CTGCTGAGCA GCTCAGAGGG GCTCCCCATG GTGGAGCTCG TCCAGAGGCC AGAGCCGTGC 
ACCCCACCCG AGATCCCTCT TGGTATCCCC GGGAGTCACT CCGGGTACCG ACGGCCCTGC 
GTGGTCCCAG CATCCCCAGA ACGACACGTG GAGGTGGATA AGGCTGTGGC ACAGACCATG 
GACTCTGTGT TTAAGGAGCT CTTGGGGAAG ACTGCCCTCC GTCAGGGCCT CGGACCGGGC 
ACCAACAACT CTTCGAGCCC CGGGCCTCGC AGCCCGAAGC CTCCAGGCTG TGGGCGCCTG 
GGCAAGAACA AAGGCTTCTC ACGGGGCCCT GGTGCCCCAG CCTCACCCTC GACCTCCCAT 
CCCCAGGGCC TAGACTCACC CTTCAAGTCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227714.1
CDS	87..2762
Translation	MATHGKLRRERGPQADYEAQVKEMRWQLGEQLRCLELQGELRRELLQELAEFMRRRAEVELEYSRGLDKLVERFSGRGGRLGGSSREHQSFRKEPSLLSPLHCWAVLLQQTRQQSRESTALSEVLGGPLAQRLSYIAEDVGRLVKKSKDLEQQLQEELLEVVSELQMRHAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLRDVMDLMDCCDTGFHLALGQVLRSYTAAESRIQASQMQGLGSLEEAVDALDPIGDKAKVLEVHAIAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDRQTIETEEVNKTLKATLQALLEVVASEDSDMLDSFQASLSTESLKSTSSDPGARQAGRRRGQQQETETFYITKLQEYVSGRSILAKLQAKHEKLQDAIQRGDKEDREVTWTQYTQRKLQKSRAPRPSSQYNQKLFGGDMEKFIQSSGQPVPLVVESCVRFINLNGLQHEGIFRVSGAQPQISEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLQPPIFPPDLFGELLASAELEAVAERVEHVAGLLARLPGPVLVVLRYLFTFLNHLAQYSDENMMDPYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLIIQPGRVFPAPAQLPGPVYEKCMAPPSASCLGDAQLEGPTGDNELELEPGTSAQEEDQEGVVEAVACFAYTGRTAQELTFQRGDILRLHERASGDWWRGEHAGARGLIPHKYITLPAGAEKLAAGQGLLAAGDLLSSSEGLPMVELVQRPEPCTPPEIPLGIPGSHSGYRRPCVVPASPERHVEVDKAVAQTMDSVFKELLGKTALRQGLGPGTNNSSSPGPRSPKPPGCGRLGKNKGFSRGPGAPASPSTSHPQGLDSPFKSH

Target ORF information:

RefSeq Version	XM_005227657.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227657.3

ORF Insert Sequence:

ATGGCCACGC ATGGGAAACT ACGCCGAGAG CGAGGGCCGC AGGCTGACTA TGAGGCGCAG 
GTCAAAGAGA TGCGCTGGCA ACTGGGTGAG CAACTGCGCT GCCTGGAGCT GCAGGGCGAA 
CTGCGACGGG AGCTGCTGCA GGAGCTGGCT GAGTTCATGC GGCGCCGCGC CGAGGTTGAG 
CTTGAGTACT CCCGGGGCCT CGACAAGCTG GTAGAGCGAT TCTCCGGCCG TGGAGGCCGC 
CTCGGGGGCA GCAGTCGGGA GCACCAGAGC TTCCGGAAGG AGCCGTCCCT GCTGTCGCCC 
TTGCACTGCT GGGCTGTGTT GTTGCAGCAG ACTCGGCAGC AGAGCCGGGA AAGCACAGCT 
CTCAGCGAGG TGCTGGGCGG GCCCCTGGCC CAGCGCCTGA GCTACATTGC CGAGGATGTG 
GGGCGCCTGG TCAAGAAGAG TAAGGATCTG GAGCAACAGC TGCAAGAGGA GCTCCTGGAG 
GTGGTGTCAG AGCTTCAGAT GCGTCACGCC AAGTTCTTGG AGCACAAACT CAAGTGTACA 
AAGGCTCGCA ATGAGTACCT GCTAAGCCTG GCCAGCGTCA ACGCCGCCGT CAGCAACTAC 
TACCTGCGAG ACGTCATGGA CCTGATGGAT TGTTGTGACA CAGGATTCCA CCTGGCCCTG 
GGCCAAGTGC TCCGGAGCTA CACGGCTGCG GAGAGCCGCA TCCAAGCCTC CCAGATGCAG 
GGCCTGGGCA GCCTGGAGGA GGCCGTGGAT GCCCTGGATC CCATAGGTGA CAAGGCCAAG 
GTGCTGGAGG TGCACGCTAT CGCCTTCTGT CCCCCGCTGC GTTTCGACTA CCAGCCCCAC 
GAGGGGGATG AGGTTGCTGA GATCCAGGTT GAGATGGAGC TGCGGGATGA GATTCTACCC 
AGAGCCCAGA ATATCCAGAG CCGCCTGGAC CGCCAGACCA TCGAGACAGA GGAGGTGAAT 
AAGACGCTGA AGGCCACACT GCAGGCCCTG CTGGAGGTGG TGGCGTCAGA AGACAGTGAC 
ATGCTCGACT CCTTCCAGGC CAGCCTGTCC ACCGAGTCCC TCAAGTCCAC CAGCTCGGAC 
CCGGGGGCTC GGCAGGCTGG CCGGCGGCGG GGCCAGCAGC AGGAGACTGA GACCTTCTAC 
ATCACGAAAC TCCAGGAGTA CGTGAGTGGA CGGAGTATCC TTGCCAAGCT GCAGGCCAAG 
CATGAGAAAC TGCAGGACGC CATCCAGCGA GGTGACAAGG AAGACCGGGA GGTGACTTGG 
ACCCAGTACA CACAGAGAAA ACTCCAGAAG AGCCGTGCCC CCCGCCCCAG CTCCCAGTAT 
AACCAGAAAC TCTTCGGGGG AGACATGGAG AAGTTTATCC AGAGCTCAGG CCAGCCCGTG 
CCCCTGGTGG TGGAGAGCTG TGTCCGCTTC ATTAACCTCA ATGGCCTGCA GCATGAGGGC 
ATCTTCCGGG TGTCGGGCGC CCAGCCCCAG ATCTCGGAGA TCCGTGATGC TTTTGAGAGA 
GGAGAGGACC CGCTGGTGGA GGGCTGCACT GCCCATGACC TGGACTCGGT GGCGGGCGTG 
CTGAAACTCT ACTTCCGGAG CTTGCAGCCC CCCATATTCC CACCAGACCT GTTTGGAGAG 
CTGCTGGCTT CTGCAGAGCT GGAGGCTGTG GCCGAGCGGG TGGAGCACGT GGCTGGCCTC 
CTGGCCCGGC TGCCCGGACC CGTGCTGGTG GTCTTGCGGT ACCTCTTCAC CTTCCTCAAC 
CACCTGGCCC AGTACAGTGA TGAGAACATG ATGGACCCCT ACAACCTGGC CGTGTGCTTT 
GGGCCGACGC TGCTGCCCGT CCCTGCTGGG CAGGACCCAG TGGCTTTGCA AGGCCGTGTC 
AACCAGCTGG TGCAGACCCT CATCATCCAG CCTGGACGGG TCTTCCCGGC CCCGGCCCAG 
CTGCCCGGCC CCGTCTATGA GAAGTGCATG GCACCACCTT CTGCCAGCTG CTTGGGGGAT 
GCCCAGTTGG AGGGCCCGAC GGGGGACAAC GAGTTGGAGC TGGAGCCGGG GACCTCAGCC 
CAGGAGGAGG ACCAGGAGGG TGTCGTGGAG GCCGTGGCTT GCTTTGCATA CACAGGCCGC 
ACGGCCCAGG AGCTGACCTT CCAGCGCGGG GACATCTTGC GGCTGCACGA GCGGGCCTCA 
GGCGACTGGT GGCGGGGCGA GCACGCGGGG GCACGGGGGC TCATCCCCCA CAAGTATATC 
ACTCTGCCTG CGGGAGCAGA GAAGCTCGCG GCGGGCCAGG GGCTGCTGGC CGCCGGGGAC 
CTGCTGAGCA GCTCAGAGGG GCTCCCCATG GTGGAGCTCG TCCAGAGGCC AGAGCCGTGC 
ACCCCACCCG AGATCCCTCT TGGTATCCCC GGGAGTCACT CCGGGTACCG ACGGCCCTGC 
GTGGTCCCAG CATCCCCAGA ACGACACGTG GAGGTGGATA AGGCTGTGGC ACAGACCATG 
GACTCTGTGT TTAAGGAGCT CTTGGGGAAG ACTGCCCTCC GTCAGGGCCT CGGACCGGGC 
ACCAACAACT CTTCGAGCCC CGGGCCTCGC AGCCCGAAGC CTCCAGGCTG TGGGCGCCTG 
GGCAAGAACA AAGGCTTCTC ACGGGGCCCT GGTGCCCCAG CCTCACCCTC GACCTCCCAT 
CCCCAGGGCC TAGACTCACC CTTCAAGTCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP4 ( XM_005227656.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227713.2 Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa238219
Clone ID Related Accession (Same CDS sequence)	XM_005227656.4
Accession Version	XM_005227656.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227656.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTGGC AACTGGGTGA GCAACTGCGC TGCCTGGAGC TGCAGGGCGA ACTGCGACGG 
GAGCTGCTGC AGGAGCTGGC TGAGTTCATG CGGCGCCGCG CCGAGGTTGA GCTTGAGTAC 
TCCCGGGGCC TCGACAAGCT GGTAGAGCGA TTCTCCGGCC GTGGAGGCCG CCTCGGGGGC 
AGCAGTCGGG AGCACCAGAG CTTCCGGAAG GAGCCGTCCC TGCTGTCGCC CTTGCACTGC 
TGGGCTGTGT TGTTGCAGCA GACTCGGCAG CAGAGCCGGG AAAGCACAGC TCTCAGCGAG 
GTGCTGGGCG GGCCCCTGGC CCAGCGCCTG AGCTACATTG CCGAGGATGT GGGGCGCCTG 
GTCAAGAAGA GTAAGGATCT GGAGCAACAG CTGCAAGAGG AGCTCCTGGA GGTGGTGTCA 
GAGCTTCAGA TGGCCAAGAA GACCTATCAG GTCTACCACA CGGAGAGCCT GAGTGCTGAG 
GCCAAACTCC GTGAAGCCGA GCGGCAGGAG GAGAAGCGGG CAGGCCGAAG TGCCACTGCC 
ACCACCACCA GCACCGAGTC TGGGCCCCTC CGCAAAGGCT CTGTCAAGAA GGGAGGGCGG 
CTGGTGGAGA AGCGTCACGC CAAGTTCTTG GAGCACAAAC TCAAGTGTAC AAAGGCTCGC 
AATGAGTACC TGCTAAGCCT GGCCAGCGTC AACGCCGCCG TCAGCAACTA CTACCTGCGA 
GACGTCATGG ACCTGATGGA TTGTTGTGAC ACAGGATTCC ACCTGGCCCT GGGCCAAGTG 
CTCCGGAGCT ACACGGCTGC GGAGAGCCGC ATCCAAGCCT CCCAGATGCA GGGCCTGGGC 
AGCCTGGAGG AGGCCGTGGA TGCCCTGGAT CCCATAGGTG ACAAGGCCAA GGTGCTGGAG 
GTGCACGCTA TCGCCTTCTG TCCCCCGCTG CGTTTCGACT ACCAGCCCCA CGAGGGGGAT 
GAGGTTGCTG AGATCCAGGT TGAGATGGAG CTGCGGGATG AGATTCTACC CAGAGCCCAG 
AATATCCAGA GCCGCCTGGA CCGCCAGACC ATCGAGACAG AGGAGGTGAA TAAGACGCTG 
AAGGCCACAC TGCAGGCCCT GCTGGAGGTG GTGGCGTCAG AAGACAGTGA CATGCTCGAC 
TCCTTCCAGG CCAGCCTGTC CACCGAGTCC CTCAAGTCCA CCAGCTCGGA CCCGGGGGCT 
CGGCAGGCTG GCCGGCGGCG GGGCCAGCAG CAGGAGACTG AGACCTTCTA CATCACGAAA 
CTCCAGGAGT ACCTGAGTGG ACGGAGTATC CTTGCCAAGC TGCAGGCCAA GCATGAGAAA 
CTGCAGGACG CCATCCAGCG AGGTGACAAG GAAGACCGGG AGGTGACTTG GACCCAGTAC 
ACACAGAGAA AACTCCAGAA GAGCCGTGCC CCCCGCCCCA GCTCCCAGTA TAACCAGAAA 
CTCTTCGGGG GAGACATGGA GAAGTTTATC CAGAGCTCAG GCCAGCCCGT GCCCCTGGTG 
GTGGAGAGCT GTGTCCGCTT CATTAACCTC AATGGCCTGC AGCATGAGGG CATCTTCCGG 
GTGTCGGGCG CCCAGCCCCA GATCTCGGAG ATCCGTGATG CTTTTGAGAG AGGAGAGGAC 
CCGCTGGTGG AGGGCTGCAC TGCCCATGAC CTGGACTCGG TGGCGGGCGT GCTGAAACTC 
TACTTCCGGA GCTTGCAGCC CCCCATATTC CCACCAGACC TGTTTGGAGA GCTGCTGGCT 
TCTGCAGAGC TGGAGGCTGT GGCCGAGCGG GTGGAGCACG TGGCTGGCCT CCTGGCCCGG 
CTGCCCGGAC CCGTGCTGGT GGTCTTGCGG TACCTCTTCA CCTTCCTCAA CCACCTGGCC 
CAGTACAGTG ATGAGAACAT GATGGACCCC TACAACCTGG CCGTGTGCTT TGGGCCGACG 
CTGCTGCCCG TCCCTGCTGG GCAGGACCCA GTGGCTTTGC AAGGCCGTGT CAACCAGCTG 
GTGCAGACCC TCATCATCCA GCCTGGACGG GTCTTCCCGG CCCCGGCCCA GCTGCCCGGC 
CCCGTCTATG AGAAGTGCAT GGCACCACCT TCTGCCAGCT GCTTGGGGGA TGCCCAGTTG 
GAGGGCCCGA CGGGGGACAA CGAGTTGGAG CTGGAGCCGG GGACCTCAGC CCAGGAGGAG 
GACCAGGAGG GTGTCGTGGA GGCCGTGGCT TGCTTTGCAT ACACAGGCCG CACGGCCCAG 
GAGCTGACCT TCCAGCGCGG GGACATCTTG CGGCTGCACG AGCGGGCCTC AGGCGACTGG 
TGGCGGGGCG AGCACGCGGG GGCACGGGGG CTCATCCCCC ACAAGTATAT CACTCTGCCT 
GCGGGAGCAG AGAAGCTCGC GGCGGGCCAG GGGCTGCTGG CCGCCGGGGA CCTGCTGAGC 
AGCTCAGAGG GGCTCCCCAT GGTGGAGCTC GTCCAGAGGC CAGAGCCGTG CACCCCACCC 
GAGATCCCTC TTGGTATCCC CGGGAGTCAC TCCGGGTACC GACGGCCCTG CGTGGTCCCA 
GCATCCCCAG AACGACACGT GGAGGTGGAT AAGGCTGTGG CACAGACCAT GGACTCTGTG 
TTTAAGGAGC TCTTGGGGAA GACTGCCCTC CGTCAGGGCC TCGGACCGGG CACCAACAAC 
TCTTCGAGCC CCGGGCCTCG CAGCCCGAAG CCTCCAGGCT GTGGGCGCCT GGGCAAGAAC 
AAAGGCTTCT CACGGGGCCC TGGTGCCCCA GCCTCACCCT CGACCTCCCA TCCCCAGGGC 
CTAGACTCAC CCTTCAAGTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227713.2
CDS	228..3014
Translation	MRWQLGEQLRCLELQGELRRELLQELAEFMRRRAEVELEYSRGLDKLVERFSGRGGRLGGSSREHQSFRKEPSLLSPLHCWAVLLQQTRQQSRESTALSEVLGGPLAQRLSYIAEDVGRLVKKSKDLEQQLQEELLEVVSELQMAKKTYQVYHTESLSAEAKLREAERQEEKRAGRSATATTTSTESGPLRKGSVKKGGRLVEKRHAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLRDVMDLMDCCDTGFHLALGQVLRSYTAAESRIQASQMQGLGSLEEAVDALDPIGDKAKVLEVHAIAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDRQTIETEEVNKTLKATLQALLEVVASEDSDMLDSFQASLSTESLKSTSSDPGARQAGRRRGQQQETETFYITKLQEYLSGRSILAKLQAKHEKLQDAIQRGDKEDREVTWTQYTQRKLQKSRAPRPSSQYNQKLFGGDMEKFIQSSGQPVPLVVESCVRFINLNGLQHEGIFRVSGAQPQISEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLQPPIFPPDLFGELLASAELEAVAERVEHVAGLLARLPGPVLVVLRYLFTFLNHLAQYSDENMMDPYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLIIQPGRVFPAPAQLPGPVYEKCMAPPSASCLGDAQLEGPTGDNELELEPGTSAQEEDQEGVVEAVACFAYTGRTAQELTFQRGDILRLHERASGDWWRGEHAGARGLIPHKYITLPAGAEKLAAGQGLLAAGDLLSSSEGLPMVELVQRPEPCTPPEIPLGIPGSHSGYRRPCVVPASPERHVEVDKAVAQTMDSVFKELLGKTALRQGLGPGTNNSSSPGPRSPKPPGCGRLGKNKGFSRGPGAPASPSTSHPQGLDSPFKSH

Target ORF information:

RefSeq Version	XM_005227656.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227656.4

ORF Insert Sequence:

ATGCGCTGGC AACTGGGTGA GCAACTGCGC TGCCTGGAGC TGCAGGGCGA ACTGCGACGG 
GAGCTGCTGC AGGAGCTGGC TGAGTTCATG CGGCGCCGCG CCGAGGTTGA GCTTGAGTAC 
TCCCGGGGCC TCGACAAGCT GGTAGAGCGA TTCTCCGGCC GTGGAGGCCG CCTCGGGGGC 
AGCAGTCGGG AGCACCAGAG CTTCCGGAAG GAGCCGTCCC TGCTGTCGCC CTTGCACTGC 
TGGGCTGTGT TGTTGCAGCA GACTCGGCAG CAGAGCCGGG AAAGCACAGC TCTCAGCGAG 
GTGCTGGGCG GGCCCCTGGC CCAGCGCCTG AGCTACATTG CCGAGGATGT GGGGCGCCTG 
GTCAAGAAGA GTAAGGATCT GGAGCAACAG CTGCAAGAGG AGCTCCTGGA GGTGGTGTCA 
GAGCTTCAGA TGGCCAAGAA GACCTATCAG GTCTACCACA CGGAGAGCCT GAGTGCTGAG 
GCCAAACTCC GTGAAGCCGA GCGGCAGGAG GAGAAGCGGG CAGGCCGAAG TGCCACTGCC 
ACCACCACCA GCACCGAGTC TGGGCCCCTC CGCAAAGGCT CTGTCAAGAA GGGAGGGCGG 
CTGGTGGAGA AGCGTCACGC CAAGTTCTTG GAGCACAAAC TCAAGTGTAC AAAGGCTCGC 
AATGAGTACC TGCTAAGCCT GGCCAGCGTC AACGCCGCCG TCAGCAACTA CTACCTGCGA 
GACGTCATGG ACCTGATGGA TTGTTGTGAC ACAGGATTCC ACCTGGCCCT GGGCCAAGTG 
CTCCGGAGCT ACACGGCTGC GGAGAGCCGC ATCCAAGCCT CCCAGATGCA GGGCCTGGGC 
AGCCTGGAGG AGGCCGTGGA TGCCCTGGAT CCCATAGGTG ACAAGGCCAA GGTGCTGGAG 
GTGCACGCTA TCGCCTTCTG TCCCCCGCTG CGTTTCGACT ACCAGCCCCA CGAGGGGGAT 
GAGGTTGCTG AGATCCAGGT TGAGATGGAG CTGCGGGATG AGATTCTACC CAGAGCCCAG 
AATATCCAGA GCCGCCTGGA CCGCCAGACC ATCGAGACAG AGGAGGTGAA TAAGACGCTG 
AAGGCCACAC TGCAGGCCCT GCTGGAGGTG GTGGCGTCAG AAGACAGTGA CATGCTCGAC 
TCCTTCCAGG CCAGCCTGTC CACCGAGTCC CTCAAGTCCA CCAGCTCGGA CCCGGGGGCT 
CGGCAGGCTG GCCGGCGGCG GGGCCAGCAG CAGGAGACTG AGACCTTCTA CATCACGAAA 
CTCCAGGAGT ACCTGAGTGG ACGGAGTATC CTTGCCAAGC TGCAGGCCAA GCATGAGAAA 
CTGCAGGACG CCATCCAGCG AGGTGACAAG GAAGACCGGG AGGTGACTTG GACCCAGTAC 
ACACAGAGAA AACTCCAGAA GAGCCGTGCC CCCCGCCCCA GCTCCCAGTA TAACCAGAAA 
CTCTTCGGGG GAGACATGGA GAAGTTTATC CAGAGCTCAG GCCAGCCCGT GCCCCTGGTG 
GTGGAGAGCT GTGTCCGCTT CATTAACCTC AATGGCCTGC AGCATGAGGG CATCTTCCGG 
GTGTCGGGCG CCCAGCCCCA GATCTCGGAG ATCCGTGATG CTTTTGAGAG AGGAGAGGAC 
CCGCTGGTGG AGGGCTGCAC TGCCCATGAC CTGGACTCGG TGGCGGGCGT GCTGAAACTC 
TACTTCCGGA GCTTGCAGCC CCCCATATTC CCACCAGACC TGTTTGGAGA GCTGCTGGCT 
TCTGCAGAGC TGGAGGCTGT GGCCGAGCGG GTGGAGCACG TGGCTGGCCT CCTGGCCCGG 
CTGCCCGGAC CCGTGCTGGT GGTCTTGCGG TACCTCTTCA CCTTCCTCAA CCACCTGGCC 
CAGTACAGTG ATGAGAACAT GATGGACCCC TACAACCTGG CCGTGTGCTT TGGGCCGACG 
CTGCTGCCCG TCCCTGCTGG GCAGGACCCA GTGGCTTTGC AAGGCCGTGT CAACCAGCTG 
GTGCAGACCC TCATCATCCA GCCTGGACGG GTCTTCCCGG CCCCGGCCCA GCTGCCCGGC 
CCCGTCTATG AGAAGTGCAT GGCACCACCT TCTGCCAGCT GCTTGGGGGA TGCCCAGTTG 
GAGGGCCCGA CGGGGGACAA CGAGTTGGAG CTGGAGCCGG GGACCTCAGC CCAGGAGGAG 
GACCAGGAGG GTGTCGTGGA GGCCGTGGCT TGCTTTGCAT ACACAGGCCG CACGGCCCAG 
GAGCTGACCT TCCAGCGCGG GGACATCTTG CGGCTGCACG AGCGGGCCTC AGGCGACTGG 
TGGCGGGGCG AGCACGCGGG GGCACGGGGG CTCATCCCCC ACAAGTATAT CACTCTGCCT 
GCGGGAGCAG AGAAGCTCGC GGCGGGCCAG GGGCTGCTGG CCGCCGGGGA CCTGCTGAGC 
AGCTCAGAGG GGCTCCCCAT GGTGGAGCTC GTCCAGAGGC CAGAGCCGTG CACCCCACCC 
GAGATCCCTC TTGGTATCCC CGGGAGTCAC TCCGGGTACC GACGGCCCTG CGTGGTCCCA 
GCATCCCCAG AACGACACGT GGAGGTGGAT AAGGCTGTGG CACAGACCAT GGACTCTGTG 
TTTAAGGAGC TCTTGGGGAA GACTGCCCTC CGTCAGGGCC TCGGACCGGG CACCAACAAC 
TCTTCGAGCC CCGGGCCTCG CAGCCCGAAG CCTCCAGGCT GTGGGCGCCT GGGCAAGAAC 
AAAGGCTTCT CACGGGGCCC TGGTGCCCCA GCCTCACCCT CGACCTCCCA TCCCCAGGGC 
CTAGACTCAC CCTTCAAGTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP4 ( XM_005227657.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227714.2 Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X2, mRNA.

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CloneID	OBa238220
Clone ID Related Accession (Same CDS sequence)	XM_005227657.4
Accession Version	XM_005227657.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227657.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC ATGGGAAACT ACGCCGAGAG CGAGGGCCGC AGGCTGACTA TGAGGCGCAG 
GTCAAAGAGA TGCGCTGGCA ACTGGGTGAG CAACTGCGCT GCCTGGAGCT GCAGGGCGAA 
CTGCGACGGG AGCTGCTGCA GGAGCTGGCT GAGTTCATGC GGCGCCGCGC CGAGGTTGAG 
CTTGAGTACT CCCGGGGCCT CGACAAGCTG GTAGAGCGAT TCTCCGGCCG TGGAGGCCGC 
CTCGGGGGCA GCAGTCGGGA GCACCAGAGC TTCCGGAAGG AGCCGTCCCT GCTGTCGCCC 
TTGCACTGCT GGGCTGTGTT GTTGCAGCAG ACTCGGCAGC AGAGCCGGGA AAGCACAGCT 
CTCAGCGAGG TGCTGGGCGG GCCCCTGGCC CAGCGCCTGA GCTACATTGC CGAGGATGTG 
GGGCGCCTGG TCAAGAAGAG TAAGGATCTG GAGCAACAGC TGCAAGAGGA GCTCCTGGAG 
GTGGTGTCAG AGCTTCAGAT GCGTCACGCC AAGTTCTTGG AGCACAAACT CAAGTGTACA 
AAGGCTCGCA ATGAGTACCT GCTAAGCCTG GCCAGCGTCA ACGCCGCCGT CAGCAACTAC 
TACCTGCGAG ACGTCATGGA CCTGATGGAT TGTTGTGACA CAGGATTCCA CCTGGCCCTG 
GGCCAAGTGC TCCGGAGCTA CACGGCTGCG GAGAGCCGCA TCCAAGCCTC CCAGATGCAG 
GGCCTGGGCA GCCTGGAGGA GGCCGTGGAT GCCCTGGATC CCATAGGTGA CAAGGCCAAG 
GTGCTGGAGG TGCACGCTAT CGCCTTCTGT CCCCCGCTGC GTTTCGACTA CCAGCCCCAC 
GAGGGGGATG AGGTTGCTGA GATCCAGGTT GAGATGGAGC TGCGGGATGA GATTCTACCC 
AGAGCCCAGA ATATCCAGAG CCGCCTGGAC CGCCAGACCA TCGAGACAGA GGAGGTGAAT 
AAGACGCTGA AGGCCACACT GCAGGCCCTG CTGGAGGTGG TGGCGTCAGA AGACAGTGAC 
ATGCTCGACT CCTTCCAGGC CAGCCTGTCC ACCGAGTCCC TCAAGTCCAC CAGCTCGGAC 
CCGGGGGCTC GGCAGGCTGG CCGGCGGCGG GGCCAGCAGC AGGAGACTGA GACCTTCTAC 
ATCACGAAAC TCCAGGAGTA CCTGAGTGGA CGGAGTATCC TTGCCAAGCT GCAGGCCAAG 
CATGAGAAAC TGCAGGACGC CATCCAGCGA GGTGACAAGG AAGACCGGGA GGTGACTTGG 
ACCCAGTACA CACAGAGAAA ACTCCAGAAG AGCCGTGCCC CCCGCCCCAG CTCCCAGTAT 
AACCAGAAAC TCTTCGGGGG AGACATGGAG AAGTTTATCC AGAGCTCAGG CCAGCCCGTG 
CCCCTGGTGG TGGAGAGCTG TGTCCGCTTC ATTAACCTCA ATGGCCTGCA GCATGAGGGC 
ATCTTCCGGG TGTCGGGCGC CCAGCCCCAG ATCTCGGAGA TCCGTGATGC TTTTGAGAGA 
GGAGAGGACC CGCTGGTGGA GGGCTGCACT GCCCATGACC TGGACTCGGT GGCGGGCGTG 
CTGAAACTCT ACTTCCGGAG CTTGCAGCCC CCCATATTCC CACCAGACCT GTTTGGAGAG 
CTGCTGGCTT CTGCAGAGCT GGAGGCTGTG GCCGAGCGGG TGGAGCACGT GGCTGGCCTC 
CTGGCCCGGC TGCCCGGACC CGTGCTGGTG GTCTTGCGGT ACCTCTTCAC CTTCCTCAAC 
CACCTGGCCC AGTACAGTGA TGAGAACATG ATGGACCCCT ACAACCTGGC CGTGTGCTTT 
GGGCCGACGC TGCTGCCCGT CCCTGCTGGG CAGGACCCAG TGGCTTTGCA AGGCCGTGTC 
AACCAGCTGG TGCAGACCCT CATCATCCAG CCTGGACGGG TCTTCCCGGC CCCGGCCCAG 
CTGCCCGGCC CCGTCTATGA GAAGTGCATG GCACCACCTT CTGCCAGCTG CTTGGGGGAT 
GCCCAGTTGG AGGGCCCGAC GGGGGACAAC GAGTTGGAGC TGGAGCCGGG GACCTCAGCC 
CAGGAGGAGG ACCAGGAGGG TGTCGTGGAG GCCGTGGCTT GCTTTGCATA CACAGGCCGC 
ACGGCCCAGG AGCTGACCTT CCAGCGCGGG GACATCTTGC GGCTGCACGA GCGGGCCTCA 
GGCGACTGGT GGCGGGGCGA GCACGCGGGG GCACGGGGGC TCATCCCCCA CAAGTATATC 
ACTCTGCCTG CGGGAGCAGA GAAGCTCGCG GCGGGCCAGG GGCTGCTGGC CGCCGGGGAC 
CTGCTGAGCA GCTCAGAGGG GCTCCCCATG GTGGAGCTCG TCCAGAGGCC AGAGCCGTGC 
ACCCCACCCG AGATCCCTCT TGGTATCCCC GGGAGTCACT CCGGGTACCG ACGGCCCTGC 
GTGGTCCCAG CATCCCCAGA ACGACACGTG GAGGTGGATA AGGCTGTGGC ACAGACCATG 
GACTCTGTGT TTAAGGAGCT CTTGGGGAAG ACTGCCCTCC GTCAGGGCCT CGGACCGGGC 
ACCAACAACT CTTCGAGCCC CGGGCCTCGC AGCCCGAAGC CTCCAGGCTG TGGGCGCCTG 
GGCAAGAACA AAGGCTTCTC ACGGGGCCCT GGTGCCCCAG CCTCACCCTC GACCTCCCAT 
CCCCAGGGCC TAGACTCACC CTTCAAGTCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227714.2
CDS	92..2767
Translation	MATHGKLRRERGPQADYEAQVKEMRWQLGEQLRCLELQGELRRELLQELAEFMRRRAEVELEYSRGLDKLVERFSGRGGRLGGSSREHQSFRKEPSLLSPLHCWAVLLQQTRQQSRESTALSEVLGGPLAQRLSYIAEDVGRLVKKSKDLEQQLQEELLEVVSELQMRHAKFLEHKLKCTKARNEYLLSLASVNAAVSNYYLRDVMDLMDCCDTGFHLALGQVLRSYTAAESRIQASQMQGLGSLEEAVDALDPIGDKAKVLEVHAIAFCPPLRFDYQPHEGDEVAEIQVEMELRDEILPRAQNIQSRLDRQTIETEEVNKTLKATLQALLEVVASEDSDMLDSFQASLSTESLKSTSSDPGARQAGRRRGQQQETETFYITKLQEYLSGRSILAKLQAKHEKLQDAIQRGDKEDREVTWTQYTQRKLQKSRAPRPSSQYNQKLFGGDMEKFIQSSGQPVPLVVESCVRFINLNGLQHEGIFRVSGAQPQISEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLQPPIFPPDLFGELLASAELEAVAERVEHVAGLLARLPGPVLVVLRYLFTFLNHLAQYSDENMMDPYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLIIQPGRVFPAPAQLPGPVYEKCMAPPSASCLGDAQLEGPTGDNELELEPGTSAQEEDQEGVVEAVACFAYTGRTAQELTFQRGDILRLHERASGDWWRGEHAGARGLIPHKYITLPAGAEKLAAGQGLLAAGDLLSSSEGLPMVELVQRPEPCTPPEIPLGIPGSHSGYRRPCVVPASPERHVEVDKAVAQTMDSVFKELLGKTALRQGLGPGTNNSSSPGPRSPKPPGCGRLGKNKGFSRGPGAPASPSTSHPQGLDSPFKSH

Target ORF information:

RefSeq Version	XM_005227657.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 4 (ARHGAP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227657.4

ORF Insert Sequence:

ATGGCCACGC ATGGGAAACT ACGCCGAGAG CGAGGGCCGC AGGCTGACTA TGAGGCGCAG 
GTCAAAGAGA TGCGCTGGCA ACTGGGTGAG CAACTGCGCT GCCTGGAGCT GCAGGGCGAA 
CTGCGACGGG AGCTGCTGCA GGAGCTGGCT GAGTTCATGC GGCGCCGCGC CGAGGTTGAG 
CTTGAGTACT CCCGGGGCCT CGACAAGCTG GTAGAGCGAT TCTCCGGCCG TGGAGGCCGC 
CTCGGGGGCA GCAGTCGGGA GCACCAGAGC TTCCGGAAGG AGCCGTCCCT GCTGTCGCCC 
TTGCACTGCT GGGCTGTGTT GTTGCAGCAG ACTCGGCAGC AGAGCCGGGA AAGCACAGCT 
CTCAGCGAGG TGCTGGGCGG GCCCCTGGCC CAGCGCCTGA GCTACATTGC CGAGGATGTG 
GGGCGCCTGG TCAAGAAGAG TAAGGATCTG GAGCAACAGC TGCAAGAGGA GCTCCTGGAG 
GTGGTGTCAG AGCTTCAGAT GCGTCACGCC AAGTTCTTGG AGCACAAACT CAAGTGTACA 
AAGGCTCGCA ATGAGTACCT GCTAAGCCTG GCCAGCGTCA ACGCCGCCGT CAGCAACTAC 
TACCTGCGAG ACGTCATGGA CCTGATGGAT TGTTGTGACA CAGGATTCCA CCTGGCCCTG 
GGCCAAGTGC TCCGGAGCTA CACGGCTGCG GAGAGCCGCA TCCAAGCCTC CCAGATGCAG 
GGCCTGGGCA GCCTGGAGGA GGCCGTGGAT GCCCTGGATC CCATAGGTGA CAAGGCCAAG 
GTGCTGGAGG TGCACGCTAT CGCCTTCTGT CCCCCGCTGC GTTTCGACTA CCAGCCCCAC 
GAGGGGGATG AGGTTGCTGA GATCCAGGTT GAGATGGAGC TGCGGGATGA GATTCTACCC 
AGAGCCCAGA ATATCCAGAG CCGCCTGGAC CGCCAGACCA TCGAGACAGA GGAGGTGAAT 
AAGACGCTGA AGGCCACACT GCAGGCCCTG CTGGAGGTGG TGGCGTCAGA AGACAGTGAC 
ATGCTCGACT CCTTCCAGGC CAGCCTGTCC ACCGAGTCCC TCAAGTCCAC CAGCTCGGAC 
CCGGGGGCTC GGCAGGCTGG CCGGCGGCGG GGCCAGCAGC AGGAGACTGA GACCTTCTAC 
ATCACGAAAC TCCAGGAGTA CCTGAGTGGA CGGAGTATCC TTGCCAAGCT GCAGGCCAAG 
CATGAGAAAC TGCAGGACGC CATCCAGCGA GGTGACAAGG AAGACCGGGA GGTGACTTGG 
ACCCAGTACA CACAGAGAAA ACTCCAGAAG AGCCGTGCCC CCCGCCCCAG CTCCCAGTAT 
AACCAGAAAC TCTTCGGGGG AGACATGGAG AAGTTTATCC AGAGCTCAGG CCAGCCCGTG 
CCCCTGGTGG TGGAGAGCTG TGTCCGCTTC ATTAACCTCA ATGGCCTGCA GCATGAGGGC 
ATCTTCCGGG TGTCGGGCGC CCAGCCCCAG ATCTCGGAGA TCCGTGATGC TTTTGAGAGA 
GGAGAGGACC CGCTGGTGGA GGGCTGCACT GCCCATGACC TGGACTCGGT GGCGGGCGTG 
CTGAAACTCT ACTTCCGGAG CTTGCAGCCC CCCATATTCC CACCAGACCT GTTTGGAGAG 
CTGCTGGCTT CTGCAGAGCT GGAGGCTGTG GCCGAGCGGG TGGAGCACGT GGCTGGCCTC 
CTGGCCCGGC TGCCCGGACC CGTGCTGGTG GTCTTGCGGT ACCTCTTCAC CTTCCTCAAC 
CACCTGGCCC AGTACAGTGA TGAGAACATG ATGGACCCCT ACAACCTGGC CGTGTGCTTT 
GGGCCGACGC TGCTGCCCGT CCCTGCTGGG CAGGACCCAG TGGCTTTGCA AGGCCGTGTC 
AACCAGCTGG TGCAGACCCT CATCATCCAG CCTGGACGGG TCTTCCCGGC CCCGGCCCAG 
CTGCCCGGCC CCGTCTATGA GAAGTGCATG GCACCACCTT CTGCCAGCTG CTTGGGGGAT 
GCCCAGTTGG AGGGCCCGAC GGGGGACAAC GAGTTGGAGC TGGAGCCGGG GACCTCAGCC 
CAGGAGGAGG ACCAGGAGGG TGTCGTGGAG GCCGTGGCTT GCTTTGCATA CACAGGCCGC 
ACGGCCCAGG AGCTGACCTT CCAGCGCGGG GACATCTTGC GGCTGCACGA GCGGGCCTCA 
GGCGACTGGT GGCGGGGCGA GCACGCGGGG GCACGGGGGC TCATCCCCCA CAAGTATATC 
ACTCTGCCTG CGGGAGCAGA GAAGCTCGCG GCGGGCCAGG GGCTGCTGGC CGCCGGGGAC 
CTGCTGAGCA GCTCAGAGGG GCTCCCCATG GTGGAGCTCG TCCAGAGGCC AGAGCCGTGC 
ACCCCACCCG AGATCCCTCT TGGTATCCCC GGGAGTCACT CCGGGTACCG ACGGCCCTGC 
GTGGTCCCAG CATCCCCAGA ACGACACGTG GAGGTGGATA AGGCTGTGGC ACAGACCATG 
GACTCTGTGT TTAAGGAGCT CTTGGGGAAG ACTGCCCTCC GTCAGGGCCT CGGACCGGGC 
ACCAACAACT CTTCGAGCCC CGGGCCTCGC AGCCCGAAGC CTCCAGGCTG TGGGCGCCTG 
GGCAAGAACA AAGGCTTCTC ACGGGGCCCT GGTGCCCCAG CCTCACCCTC GACCTCCCAT 
CCCCAGGGCC TAGACTCACC CTTCAAGTCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ARHGAP4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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