Ckm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ckm
Entrez Gene ID	24265
Full Name	creatine kinase, M-type
Synonyms	Ckmm
General protein information	Preferred Names creatine kinase, M-type Names creatine kinase M-type M-CK creatine kinase M chain creatine kinase, muscle
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	catalyzes regeneration of ATP; expression is induced by p53 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017588776.1	XP_017444265.1	creatine kinase M-type isoform X1
NM_012530.2	NP_036662.1	creatine kinase M-type

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CKM	XP_001163660.2
Rattus norvegicus (Norway rat)	Ckm	NP_036662.1
Homo sapiens (human)	CKM	NP_001815.2
Canis lupus familiaris (dog)	CKM	XP_005616506.1
Bos taurus (cattle)	CKM	NP_777198.2
Mus musculus (house mouse)	Ckm	NP_031736.1
Gallus gallus (chicken)	CKM	NP_990838.1
Xenopus tropicalis (tropical clawed frog)	ckm	NP_989374.1
Danio rerio (zebrafish)	ckmb	NP_001099153.1
Danio rerio (zebrafish)	ckma	NP_571007.1

Gene Ontology
Bibliography

Related articles in PubMed

Decreased creatine kinase is linked to diastolic dysfunction in rats with right heart failure induced by pulmonary artery hypertension.
Fowler ED, Benoist D, Drinkhill MJ, Stones R, Helmes M, W?st RC, Stienen GJ, Steele DS, White E
Journal of molecular and cellular cardiology861-8(2015 Sep)

Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity.
Demonbreun AR, Rossi AE, Alvarez MG, Swanson KE, Deveaux HK, Earley JU, Hadhazy M, Vohra R, Walter GA, Pytel P, McNally EM
The American journal of pathology184(1)248-59(2014 Jan)

Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomyopathy.
Diguet N, Mallat Y, Ladouce R, Clodic G, Prola A, Tritsch E, Blanc J, Larcher JC, Delcayre C, Samuel JL, Friguet B, Bolbach G, Li Z, Mericskay M
The Journal of biological chemistry286(40)35007-19(2011 Oct)

Diaphragm and cardiac mitochondrial creatine kinases are impaired in sepsis.
Callahan LA, Supinski GS
Journal of applied physiology (Bethesda, Md. : 1985)102(1)44-53(2007 Jan)

Regionally perfused taurine. Part I. Minimizes lactic acidosis and preserves CKMB and myocardial contractility after ischemia/reperfusion.
Oriyanhan W, Miyamoto TA, Yamazaki K, Miwa S, Takaba K, Ikeda T, Komeda M
Advances in experimental medicine and biology583271-88(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Creatine kinase inhibition also mimics a definitive characteristic of heart failure, the inability to respond to increased demand. Novel therapies for pulmonary artery hypertension are needed.
Title: Decreased creatine kinase is linked to diastolic dysfunction in rats with right heart failure induced by pulmonary artery hypertension.

strong down-regulation of MCK activity contributes to F-actin instability and induces post-translational modification of alphaB-crystallin and desmin
Title: Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomyopathy.

Endotoxin-induced sepsis markedly reduced diaphragm and cardiac mitochondrial creatine kinase activity
Title: Diaphragm and cardiac mitochondrial creatine kinases are impaired in sepsis.

Data show that taurine minimizes lactic acidosis and preserves creatine kinase activity and myocardial contractility after ischemia/reperfusion.
Title: Regionally perfused taurine. Part I. Minimizes lactic acidosis and preserves CKMB and myocardial contractility after ischemia/reperfusion.

Characteristic fatigue resistance of extraocular muscles does not depend on creatine kinase(CK) activity. Expression of known CK isoforms is downregulated in these muscles.
Title: Fatigue resistance of rat extraocular muscles does not depend on creatine kinase activity.

The following Ckm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ckm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12322	NM_012530.2 Latest version!	Rattus norvegicus creatine kinase, M-type (Ckm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa56380	XM_017588776.1 Latest version!	Rattus norvegicus creatine kinase, M-type (Ckm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ckm ( NM_012530.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036662.1 Rattus norvegicus creatine kinase, M-type (Ckm), mRNA.

Price & Availability↑

CloneID	ORa12322
Clone ID Related Accession (Same CDS sequence)	NM_012530.2
Accession Version	NM_012530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	creatine kinase M-type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM056549.1, DN931989.1, FM053193.1 and CR458343.1. On Nov 28, 2013 this sequence version replaced NM_012530.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ216665.1, M10140.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTCG GCAACACCCA CAACAAGTTC AAGCTGAACT ACAAGTCGCA GGAGGAGTAC 
CCAGACCTCA GCAAACACAA CAACCACATG GCCAAGGTGC TGACTCCTGA CCTCTACAAT 
AAGCTTCGAG ACAAGGAGAC CCCATCCGGC TTCACTCTGG ACGATGTCAT CCAGACTGGG 
GTGGACAACC CGGGTCACCC TTTCATCATG ACGGTCGGCT GTGTGGCCGG CGATGAGGAG 
TCCTACACTG TTTTCAAGGA CCTGTTTGAC CCCATCATCC AGGACCGCCA TGGTGGCTTC 
AAACCCACAG ACAAGCATAA GACCGACCTC AACCACGAGA ACCTCAAGGG TGGAGACGAT 
CTGGACCCCA ACTATGTGCT GAGCAGCCGC GTGCGCACTG GCCGCAGCAT CAAGGGCTAC 
ACTCTGCCCC CGCACTGCTC CCGCGGAGAG CGCCGTGCTG TAGAGAAGCT GTCCGTGGAA 
GCTCTCAACA GCCTGACAGG CGAGTTCAAG GGCAAATACT ACCCTCTGAA GAGCATGACA 
GAGCAGGAGC AGCAGCAGCT CATTGATGAC CACTTCCTGT TTGACAAGCC CGTGTCACCT 
CTGCTGCTGG CCTCAGGCAT GGCCCGAGAC TGGCCCGACG CCCGTGGCAT CTGGCACAAC 
GACAACAAAA GCTTCCTGGT GTGGGTGAAT GAGGAGGACC ACCTTCGCGT GATCTCCATG 
GAGAAAGGAG GCAATATGAA GGAAGTTTTC CGCCGCTTCT GCGTGGGCCT GCAGAAGATT 
GAGGAGATCT TCAAGAAGGC TGGTCACCCC TTCATGTGGA ACGAGCACCT GGGCTACGTG 
CTCACCTGCC CCTCCAACCT GGGCACCGGG CTGCGCGGAG GCGTGCACGT GAAGCTGGCG 
AACCTGAGCA AGCACCCCAA GTTTGAGGAG ATTCTCACCC GCCTTCGTCT GCAGAAGCGC 
GGCACAGGTG GCGTGGACAC CGCTGCGGTG GGCGCCGTGT TCGACATCTC CAACGCCGAT 
CGGCTGGGCT CTTCCGAGGT CGAACAGGTG CAGCTGGTGG TGGATGGCGT AAAGCTTATG 
GTGGAGATGG AGAAGAAGCT GGAAAAAGGC CAGTCCATCG ACGACATGAT CCCTGCGCAG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036662.1
CDS	97..1242
Translation	MPFGNTHNKFKLNYKSQEEYPDLSKHNNHMAKVLTPDLYNKLRDKETPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEESYTVFKDLFDPIIQDRHGGFKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEQEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNEHLGYVLTCPSNLGTGLRGGVHVKLANLSKHPKFEEILTRLRLQKRGTGGVDTAAVGAVFDISNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK

Target ORF information:

RefSeq Version	NM_012530.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus creatine kinase, M-type (Ckm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012530.2

ORF Insert Sequence:

ATGCCGTTCG GCAACACCCA CAACAAGTTC AAGCTGAACT ACAAGTCGCA GGAGGAGTAC 
CCAGACCTCA GCAAACACAA CAACCACATG GCCAAGGTGC TGACTCCTGA CCTCTACAAT 
AAGCTTCGAG ACAAGGAGAC CCCATCCGGC TTCACTCTGG ACGATGTCAT CCAGACTGGG 
GTGGACAACC CGGGTCACCC TTTCATCATG ACGGTCGGCT GTGTGGCCGG CGATGAGGAG 
TCCTACACTG TTTTCAAGGA CCTGTTTGAC CCCATCATCC AGGACCGCCA TGGTGGCTTC 
AAACCCACAG ACAAGCATAA GACCGACCTC AACCACGAGA ACCTCAAGGG TGGAGACGAT 
CTGGACCCCA ACTATGTGCT GAGCAGCCGC GTGCGCACTG GCCGCAGCAT CAAGGGCTAC 
ACTCTGCCCC CGCACTGCTC CCGCGGAGAG CGCCGTGCTG TAGAGAAGCT GTCCGTGGAA 
GCTCTCAACA GCCTGACAGG CGAGTTCAAG GGCAAATACT ACCCTCTGAA GAGCATGACA 
GAGCAGGAGC AGCAGCAGCT CATTGATGAC CACTTCCTGT TTGACAAGCC CGTGTCACCT 
CTGCTGCTGG CCTCAGGCAT GGCCCGAGAC TGGCCCGACG CCCGTGGCAT CTGGCACAAC 
GACAACAAAA GCTTCCTGGT GTGGGTGAAT GAGGAGGACC ACCTTCGCGT GATCTCCATG 
GAGAAAGGAG GCAATATGAA GGAAGTTTTC CGCCGCTTCT GCGTGGGCCT GCAGAAGATT 
GAGGAGATCT TCAAGAAGGC TGGTCACCCC TTCATGTGGA ACGAGCACCT GGGCTACGTG 
CTCACCTGCC CCTCCAACCT GGGCACCGGG CTGCGCGGAG GCGTGCACGT GAAGCTGGCG 
AACCTGAGCA AGCACCCCAA GTTTGAGGAG ATTCTCACCC GCCTTCGTCT GCAGAAGCGC 
GGCACAGGTG GCGTGGACAC CGCTGCGGTG GGCGCCGTGT TCGACATCTC CAACGCCGAT 
CGGCTGGGCT CTTCCGAGGT CGAACAGGTG CAGCTGGTGG TGGATGGCGT AAAGCTTATG 
GTGGAGATGG AGAAGAAGCT GGAAAAAGGC CAGTCCATCG ACGACATGAT CCCTGCGCAG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ckm ( XM_017588776.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444265.1 Rattus norvegicus creatine kinase, M-type (Ckm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56380
Clone ID Related Accession (Same CDS sequence)	XM_017588776.1
Accession Version	XM_017588776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	creatine kinase M-type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTCG GCTGTGTGGC CGGCGATGAG GAGTCCTACA CTGTTTTCAA GGACCTGTTT 
GACCCCATCA TCCAGGACCG CCATGGTGGC TTCAAACCCA CAGACAAGCA TAAGACCGAC 
CTCAACCACG AGAACCTCAA GGGTGGAGAC GATCTGGACC CCAACTATGT GCTGAGCAGC 
CGCGTGCGCA CTGGCCGCAG CATCAAGGGC TACACTCTGC CCCCGCACTG CTCCCGCGGA 
GAGCGCCGTG CTGTAGAGAA GCTGTCCGTG GAAGCTCTCA ACAGCCTGAC AGGCGAGTTC 
AAGGGCAAAT ACTACCCTCT GAAGAGCATG ACAGAGCAGG AGCAGCAGCA GCTCATTGAT 
GACCACTTCC TGTTTGACAA GCCCGTGTCA CCTCTGCTGC TGGCCTCAGG CATGGCCCGA 
GACTGGCCCG ACGCCCGTGG CATCTGGCAC AACGACAACA AAAGCTTCCT GGTGTGGGTG 
AATGAGGAGG ACCACCTTCG CGTGATCTCC ATGGAGAAAG GAGGCAATAT GAAGGAAGTT 
TTCCGCCGCT TCTGCGTGGG CCTGCAGAAG ATTGAGGAGA TCTTCAAGAA GGCTGGTCAC 
CCCTTCATGT GGAACGAGCA CCTGGGCTAC GTGCTCACCT GCCCCTCCAA CCTGGGCACC 
GGGCTGCGCG GAGGCGTGCA CGTGAAGCTG GCGAACCTGA GCAAGCACCC CAAGTTTGAG 
GAGATTCTCA CCCGCCTTCG TCTGCAGAAG CGCGGCACAG GTGGCGTGGA CACCGCTGCG 
GTGGGCGCCG TGTTCGACAT CTCCAACGCC GATCGGCTGG GCTCTTCCGA GGTCGAACAG 
GTGCAGCTGG TGGTGGATGG CGTAAAGCTT ATGGTGGAGA TGGAGAAGAA GCTGGAAAAA 
GGCCAGTCCA TCGACGACAT GATCCCTGCG CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444265.1
CDS	170..1108
Translation	MTVGCVAGDEESYTVFKDLFDPIIQDRHGGFKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEQEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNEHLGYVLTCPSNLGTGLRGGVHVKLANLSKHPKFEEILTRLRLQKRGTGGVDTAAVGAVFDISNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK

Target ORF information:

RefSeq Version	XM_017588776.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus creatine kinase, M-type (Ckm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588776.1

ORF Insert Sequence:

ATGACGGTCG GCTGTGTGGC CGGCGATGAG GAGTCCTACA CTGTTTTCAA GGACCTGTTT 
GACCCCATCA TCCAGGACCG CCATGGTGGC TTCAAACCCA CAGACAAGCA TAAGACCGAC 
CTCAACCACG AGAACCTCAA GGGTGGAGAC GATCTGGACC CCAACTATGT GCTGAGCAGC 
CGCGTGCGCA CTGGCCGCAG CATCAAGGGC TACACTCTGC CCCCGCACTG CTCCCGCGGA 
GAGCGCCGTG CTGTAGAGAA GCTGTCCGTG GAAGCTCTCA ACAGCCTGAC AGGCGAGTTC 
AAGGGCAAAT ACTACCCTCT GAAGAGCATG ACAGAGCAGG AGCAGCAGCA GCTCATTGAT 
GACCACTTCC TGTTTGACAA GCCCGTGTCA CCTCTGCTGC TGGCCTCAGG CATGGCCCGA 
GACTGGCCCG ACGCCCGTGG CATCTGGCAC AACGACAACA AAAGCTTCCT GGTGTGGGTG 
AATGAGGAGG ACCACCTTCG CGTGATCTCC ATGGAGAAAG GAGGCAATAT GAAGGAAGTT 
TTCCGCCGCT TCTGCGTGGG CCTGCAGAAG ATTGAGGAGA TCTTCAAGAA GGCTGGTCAC 
CCCTTCATGT GGAACGAGCA CCTGGGCTAC GTGCTCACCT GCCCCTCCAA CCTGGGCACC 
GGGCTGCGCG GAGGCGTGCA CGTGAAGCTG GCGAACCTGA GCAAGCACCC CAAGTTTGAG 
GAGATTCTCA CCCGCCTTCG TCTGCAGAAG CGCGGCACAG GTGGCGTGGA CACCGCTGCG 
GTGGGCGCCG TGTTCGACAT CTCCAACGCC GATCGGCTGG GCTCTTCCGA GGTCGAACAG 
GTGCAGCTGG TGGTGGATGG CGTAAAGCTT ATGGTGGAGA TGGAGAAGAA GCTGGAAAAA 
GGCCAGTCCA TCGACGACAT GATCCCTGCG CAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Decreased creatine kinase is linked to diastolic dysfunction in rats with right heart failure induced by pulmonary artery hypertension.
Journal of molecular and cellular cardiology861-8(2015 Sep)
Fowler ED,Benoist D,Drinkhill MJ,Stones R,Helmes M,W?st RC,Stienen GJ,Steele DS,White E

Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity.
The American journal of pathology184(1)248-59(2014 Jan)
Demonbreun AR,Rossi AE,Alvarez MG,Swanson KE,Deveaux HK,Earley JU,Hadhazy M,Vohra R,Walter GA,Pytel P,McNally EM

Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomyopathy.
The Journal of biological chemistry286(40)35007-19(2011 Oct)
Diguet N,Mallat Y,Ladouce R,Clodic G,Prola A,Tritsch E,Blanc J,Larcher JC,Delcayre C,Samuel JL,Friguet B,Bolbach G,Li Z,Mericskay M

Diaphragm and cardiac mitochondrial creatine kinases are impaired in sepsis.
Journal of applied physiology (Bethesda, Md. : 1985)102(1)44-53(2007 Jan)
Callahan LA,Supinski GS

Regionally perfused taurine. Part I. Minimizes lactic acidosis and preserves CKMB and myocardial contractility after ischemia/reperfusion.
Advances in experimental medicine and biology583271-88(2006)
Oriyanhan W,Miyamoto TA,Yamazaki K,Miwa S,Takaba K,Ikeda T,Komeda M

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Ckm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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