ckmb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ckmb
Entrez Gene ID	794752
Full Name	creatine kinase, muscle b
Synonyms	ckm3,wu:fb55e09,zgc:64204,zgc:92070
General protein information	Preferred Names creatine kinase, muscle b Names creatine kinase M-type
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005157593.1	XP_005157650.1	creatine kinase M-type isoform X1
NM_001105683.1	NP_001099153.1	creatine kinase M-type

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CKM	XP_001163660.2
Rattus norvegicus (Norway rat)	Ckm	NP_036662.1
Homo sapiens (human)	CKM	NP_001815.2
Canis lupus familiaris (dog)	CKM	XP_005616506.1
Bos taurus (cattle)	CKM	NP_777198.2
Mus musculus (house mouse)	Ckm	NP_031736.1
Gallus gallus (chicken)	CKM	NP_990838.1
Xenopus tropicalis (tropical clawed frog)	ckm	NP_989374.1
Danio rerio (zebrafish)	ckmb	NP_001099153.1
Danio rerio (zebrafish)	ckma	NP_571007.1

Gene Ontology
Bibliography

Related articles in PubMed

Exposure to cocaine and its main metabolites altered the protein profile of zebrafish embryos.
Parolini M, Bini L, Magni S, Rizzo A, Ghilardi A, Landi C, Armini A, Del Giacco L, Binelli A
Environmental pollution (Barking, Essex : 1987)232603-614(2018 Jan)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Comprehensive and quantitative proteomic analyses of zebrafish plasma reveals conserved protein profiles between genders and between zebrafish and human.
Li C, Tan XF, Lim TK, Lin Q, Gong Z
Scientific reports624329(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ckmb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ckmb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36286	NM_001105683.1 Latest version!	Danio rerio creatine kinase, muscle b (ckmb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa20488	XM_005157593.1 Latest version!	Danio rerio creatine kinase, muscle b (ckmb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ckmb ( NM_001105683.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001099153.1 Danio rerio creatine kinase, muscle b (ckmb), mRNA.

Price & Availability↑

CloneID	ODa36286
Clone ID Related Accession (Same CDS sequence)	NM_001105683.1
Accession Version	NM_001105683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	creatine kinase M-type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC153631.1. On or before Mar 23, 2008 this sequence version replaced NM_213121.3, XM_001333418.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC153631.1, EE706984.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGACTAAGA ACTGCAACAA CGATTACAAG ATGAAGTTTG CTGTGGATGA GGAGTTTCCT 
GACCTCTCCC AGCACAACAA CCATATGTCC AAGGTCCTCA CCAAGGACAT CTACAACAAG 
CTCAGGAGCA AGTCAACCCC CAGTGGATTC ACCCTTGATG ACTGCATCCA GACTGGTGTG 
GACAACCCTG GTCACCCCTT CATCATGACT GTCGGCTGTG TGGCTGGGGA TGAGGAGTCC 
TATGAAGCCT TCAAGGAGTT GTTCGACCCC GTCATTTCTG ACCGTCATGG TGGCTATAAG 
CCCACCGACA AGCATCTTAC TGATCTGAAC TGGGAGAACC TGAAGGGTGG TGATGATCTT 
GACCCCAACT ACGTTCTGAG CAGCCGCGTA CGTACCGGCC GCAGCATCAA GGGATTCACC 
CTGCCTCCTC ACAACAGCCG TGGTGAGCGC AGAGCTGTGG AGAAGCTGTC CATTGAGGCT 
CTGAACAGCC TGGATGGTGA GTTCAAGGGC AAGTACTACC CACTGAAGGA CATGACTGAC 
AAGGAGCAGG AGCAGCTCAT TGCTGACCAC TTCCTGTTTG ACAAGCCTGT GTCCCCCCTG 
CTGTTGGCTG CTGGCATGGC CCGTGACTGG TCTGACGGTA GAGGTATCTG GCACAACGAC 
AACAAGACCT TCCTTGTGTG GGTGAACGAG GAGGATCACC TCCGTGTCAT CTCTATGCAG 
AAGGGTGGCA ACATGAAGGA GGTCTTCAAG AGGTTCTGCG TTGGCCTGCA GAAGATTGAG 
GATGTTTTCA AGAAGCACAA CCACGGTTTC ATGTGGAACG AGCATCTTGG TTTCATCTTG 
ACCTGCCCCT CTAACTTGGG TACCGGTCTG CGCGGTGGTG TCCACGTCAA GCTGCCCAAA 
CTCAGCACAC ATGCCAAATT TGAGGAGATC CTGACCAGAC TGCGTCTTCA GAAGCGTGGC 
ACAGGTGGTG TGGACACAGC CTCCGTCGGT GGTGTGTTCG ACATCTCAAA CGCTGACCGT 
CTGGGCTCCT CTGAGGTGCA GCAGGTGCAG CTGGTGGTCG ATGGTGTGAA ACTCATGGTT 
GAAATGGAAA AGAAACTGGA GAAGGGCGAG TCCATCGATG ACATGATCCC TGCCCAGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099153.1
CDS	15..1157
Translation	MTKNCNNDYKMKFAVDEEFPDLSQHNNHMSKVLTKDIYNKLRSKSTPSGFTLDDCIQTGVDNPGHPFIMTVGCVAGDEESYEAFKELFDPVISDRHGGYKPTDKHLTDLNWENLKGGDDLDPNYVLSSRVRTGRSIKGFTLPPHNSRGERRAVEKLSIEALNSLDGEFKGKYYPLKDMTDKEQEQLIADHFLFDKPVSPLLLAAGMARDWSDGRGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFKRFCVGLQKIEDVFKKHNHGFMWNEHLGFILTCPSNLGTGLRGGVHVKLPKLSTHAKFEEILTRLRLQKRGTGGVDTASVGGVFDISNADRLGSSEVQQVQLVVDGVKLMVEMEKKLEKGESIDDMIPAQK

Target ORF information:

RefSeq Version	NM_001105683.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio creatine kinase, muscle b (ckmb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105683.1

ORF Insert Sequence:

ATGACTAAGA ACTGCAACAA CGATTACAAG ATGAAGTTTG CTGTGGATGA GGAGTTTCCT 
GACCTCTCCC AGCACAACAA CCATATGTCC AAGGTCCTCA CCAAGGACAT CTACAACAAG 
CTCAGGAGCA AGTCAACCCC CAGTGGATTC ACCCTTGATG ACTGCATCCA GACTGGTGTG 
GACAACCCTG GTCACCCCTT CATCATGACT GTCGGCTGTG TGGCTGGGGA TGAGGAGTCC 
TATGAAGCCT TCAAGGAGTT GTTCGACCCC GTCATTTCTG ACCGTCATGG TGGCTATAAG 
CCCACCGACA AGCATCTTAC TGATCTGAAC TGGGAGAACC TGAAGGGTGG TGATGATCTT 
GACCCCAACT ACGTTCTGAG CAGCCGCGTA CGTACCGGCC GCAGCATCAA GGGATTCACC 
CTGCCTCCTC ACAACAGCCG TGGTGAGCGC AGAGCTGTGG AGAAGCTGTC CATTGAGGCT 
CTGAACAGCC TGGATGGTGA GTTCAAGGGC AAGTACTACC CACTGAAGGA CATGACTGAC 
AAGGAGCAGG AGCAGCTCAT TGCTGACCAC TTCCTGTTTG ACAAGCCTGT GTCCCCCCTG 
CTGTTGGCTG CTGGCATGGC CCGTGACTGG TCTGACGGTA GAGGTATCTG GCACAACGAC 
AACAAGACCT TCCTTGTGTG GGTGAACGAG GAGGATCACC TCCGTGTCAT CTCTATGCAG 
AAGGGTGGCA ACATGAAGGA GGTCTTCAAG AGGTTCTGCG TTGGCCTGCA GAAGATTGAG 
GATGTTTTCA AGAAGCACAA CCACGGTTTC ATGTGGAACG AGCATCTTGG TTTCATCTTG 
ACCTGCCCCT CTAACTTGGG TACCGGTCTG CGCGGTGGTG TCCACGTCAA GCTGCCCAAA 
CTCAGCACAC ATGCCAAATT TGAGGAGATC CTGACCAGAC TGCGTCTTCA GAAGCGTGGC 
ACAGGTGGTG TGGACACAGC CTCCGTCGGT GGTGTGTTCG ACATCTCAAA CGCTGACCGT 
CTGGGCTCCT CTGAGGTGCA GCAGGTGCAG CTGGTGGTCG ATGGTGTGAA ACTCATGGTT 
GAAATGGAAA AGAAACTGGA GAAGGGCGAG TCCATCGATG ACATGATCCC TGCCCAGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckmb ( XM_005157593.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157650.1 Danio rerio creatine kinase, muscle b (ckmb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20488
Clone ID Related Accession (Same CDS sequence)	XM_005157593.1
Accession Version	XM_005157593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	creatine kinase M-type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAGA ACTGCAACAA CGATTACAAG ATGAAGTTTG CTGTGGATGA GGAGTTTCCT 
GACCTCTCCC AGCACAACAA CCATATGTCC AAGGTCCTCA CCAAGGACAT CTACAACAAG 
CTCAGGAGCA AGTCAACCCC CAGTGGATTC ACCCTTGATG ACTGCATCCA GACTGGTGTG 
GACAACCCTG GTCACCCCTT CATCATGACT GTCGGCTGTG TGGCTGGGGA TGAGGAGTCC 
TATGAAGCCT TCAAGGAGTT GTTCGACCCC GTCATTTCTG ACCGTCATGG TGGCTATAAG 
CCCACCGACA AGCATCTTAC TGATCTGAAC TGGGAGAACC TGAAGGGTGG TGATGATCTT 
GACCCCAACT ACGTTCTGAG CAGCCGCGTA CGTACCGGCC GCAGCATCAA GGGATTCACC 
CTGCCTCCTC ACAACAGCCG TGGTGAGCGC AGAGCTGTGG AGAAGCTGTC CATTGAGGCT 
CTGAACAGCC TGGATGGTGA GTTCAAGGGC AAGTACTACC CACTGAAGGA CATGACTGAC 
AAGGAGCAGG AGCAGCTCAT TGCTGACCAC TTCCTGTTTG ACAAGCCTGT GTCCCCCCTG 
CTGTTGGCTG CTGGCATGGC CCGTGACTGG CCTGACGGTA GAGGTATCTG GCACAACGAC 
AACAAGACCT TCCTTGTGTG GGTGAACGAG GAGGATCACC TCCGTGTCAT CTCTATGCAG 
AAGGGTGGCA ACATGAAGGA GGTCTTCAAG AGGTTCTGCG TTGGCCTGCA GAAGATTGAG 
GATGTTTTCA AGAAGCACAA CCACGGTTTC ATGTGGAACG AGCATCTTGG TTTCATCTTG 
ACCTGCCCCT CTAACTTGGG TACCGGTCTG CGCGGTGGTG TCCACGTCAA GCTGCCCAAA 
CTCAGCACAC ATGCCAAATT TGAGGAGATC CTGACCAGAC TGCGTCTTCA GAAGCGTGGC 
ACAGGTGGTG TGGACACAGC CTCCGTCGGT GGTGTGTTCG ACATCTCAAA CGCTGACCGT 
CTGGGCTCCT CTGAGGTGCA GCAGGTGCAG CTGGTGGTCG ATGGTGTGAA ACTCATGGTT 
GAAATGGAAA AGAAACTGGA GAAGGGCGAG TCCATCGATG ACATGATCCC TGCCCAGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157650.1
CDS	195..1337
Translation	MTKNCNNDYKMKFAVDEEFPDLSQHNNHMSKVLTKDIYNKLRSKSTPSGFTLDDCIQTGVDNPGHPFIMTVGCVAGDEESYEAFKELFDPVISDRHGGYKPTDKHLTDLNWENLKGGDDLDPNYVLSSRVRTGRSIKGFTLPPHNSRGERRAVEKLSIEALNSLDGEFKGKYYPLKDMTDKEQEQLIADHFLFDKPVSPLLLAAGMARDWPDGRGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFKRFCVGLQKIEDVFKKHNHGFMWNEHLGFILTCPSNLGTGLRGGVHVKLPKLSTHAKFEEILTRLRLQKRGTGGVDTASVGGVFDISNADRLGSSEVQQVQLVVDGVKLMVEMEKKLEKGESIDDMIPAQK

Target ORF information:

RefSeq Version	XM_005157593.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio creatine kinase, muscle b (ckmb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157593.1

ORF Insert Sequence:

ATGACTAAGA ACTGCAACAA CGATTACAAG ATGAAGTTTG CTGTGGATGA GGAGTTTCCT 
GACCTCTCCC AGCACAACAA CCATATGTCC AAGGTCCTCA CCAAGGACAT CTACAACAAG 
CTCAGGAGCA AGTCAACCCC CAGTGGATTC ACCCTTGATG ACTGCATCCA GACTGGTGTG 
GACAACCCTG GTCACCCCTT CATCATGACT GTCGGCTGTG TGGCTGGGGA TGAGGAGTCC 
TATGAAGCCT TCAAGGAGTT GTTCGACCCC GTCATTTCTG ACCGTCATGG TGGCTATAAG 
CCCACCGACA AGCATCTTAC TGATCTGAAC TGGGAGAACC TGAAGGGTGG TGATGATCTT 
GACCCCAACT ACGTTCTGAG CAGCCGCGTA CGTACCGGCC GCAGCATCAA GGGATTCACC 
CTGCCTCCTC ACAACAGCCG TGGTGAGCGC AGAGCTGTGG AGAAGCTGTC CATTGAGGCT 
CTGAACAGCC TGGATGGTGA GTTCAAGGGC AAGTACTACC CACTGAAGGA CATGACTGAC 
AAGGAGCAGG AGCAGCTCAT TGCTGACCAC TTCCTGTTTG ACAAGCCTGT GTCCCCCCTG 
CTGTTGGCTG CTGGCATGGC CCGTGACTGG CCTGACGGTA GAGGTATCTG GCACAACGAC 
AACAAGACCT TCCTTGTGTG GGTGAACGAG GAGGATCACC TCCGTGTCAT CTCTATGCAG 
AAGGGTGGCA ACATGAAGGA GGTCTTCAAG AGGTTCTGCG TTGGCCTGCA GAAGATTGAG 
GATGTTTTCA AGAAGCACAA CCACGGTTTC ATGTGGAACG AGCATCTTGG TTTCATCTTG 
ACCTGCCCCT CTAACTTGGG TACCGGTCTG CGCGGTGGTG TCCACGTCAA GCTGCCCAAA 
CTCAGCACAC ATGCCAAATT TGAGGAGATC CTGACCAGAC TGCGTCTTCA GAAGCGTGGC 
ACAGGTGGTG TGGACACAGC CTCCGTCGGT GGTGTGTTCG ACATCTCAAA CGCTGACCGT 
CTGGGCTCCT CTGAGGTGCA GCAGGTGCAG CTGGTGGTCG ATGGTGTGAA ACTCATGGTT 
GAAATGGAAA AGAAACTGGA GAAGGGCGAG TCCATCGATG ACATGATCCC TGCCCAGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exposure to cocaine and its main metabolites altered the protein profile of zebrafish embryos.
Environmental pollution (Barking, Essex : 1987)232603-614(2018 Jan)
Parolini M,Bini L,Magni S,Rizzo A,Ghilardi A,Landi C,Armini A,Del Giacco L,Binelli A

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Comprehensive and quantitative proteomic analyses of zebrafish plasma reveals conserved protein profiles between genders and between zebrafish and human.
Scientific reports624329(2016 Apr)
Li C,Tan XF,Lim TK,Lin Q,Gong Z

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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ckmb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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