Atp4b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp4b
Entrez Gene ID	24217
Full Name	ATPase H+/K+ transporting beta subunit
Synonyms	S76401S1
General protein information	Preferred Names ATPase H+/K+ transporting beta subunit Names potassium-transporting ATPase subunit beta ATPase H+K+-transporting beta / (gastric HK-ATPase beta subunit) defined by SSR ATPase, H+,K+-transporting, beta / (gastric H,K-ATPase beta subunit), defined by SSR ATPase, H+/K+ exchanging, beta polypeptide ATPase, H+/K+ transporting, beta polypeptide gastric H(+)/K(+) ATPase subunit beta proton pump beta chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5
Summary	subunit of ATP dependent pump that catalyzes the exchange of H+ and K+; involved in secretion of stomach acids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012510.2	NP_036642.2	potassium-transporting ATPase subunit beta

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ATP4B	NP_001193069.1
Gallus gallus (chicken)	ATP4B	NP_989749.1
Mus musculus (house mouse)	Atp4b	NP_033854.1
Xenopus tropicalis (tropical clawed frog)	atp4b	NP_001005806.1
Homo sapiens (human)	ATP4B	NP_000696.1
Rattus norvegicus (Norway rat)	Atp4b	NP_036642.2
Pan troglodytes (chimpanzee)	ATP4B	XP_001146058.1
Canis lupus familiaris (dog)	ATP4B	NP_001003328.1
	ATP4B	XP_001088163.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of the Histamine 1 Receptor Antagonist Cetirizine on the Osteoporotic Phenotype in H(+) /K(+) ATPase Beta Subunit KO Mice.
Aasar?d KM, Stunes AK, Mosti MP, Ramezanzadehkoldeh M, Viggaklev BI, Reseland JE, Skallerud BH, Fossmark R, Syversen U
Journal of cellular biochemistry117(9)2089-96(2016 09)

Remodeling of the residual gastric mucosa after roux-en-y gastric bypass or vertical sleeve gastrectomy in diet-induced obese rats.
Arapis K, Cavin JB, Gillard L, Cluzeaud F, Lett?ron P, Ducroc R, Le Beyec J, Hourseau M, Couvelard A, Marmuse JP, Le Gall M, Bado A
PloS one10(3)e0121414(2015)

Intragenic DNA methylation concomitant with repression of ATP4B and ATP4A gene expression in gastric cancer is a potential serum biomarker.
Raja UM, Gopal G, Rajkumar T
Asian Pacific journal of cancer prevention : APJCP13(11)5563-8(2012)

Long-term gastric changes in achlorhydric H(+)/K(+)-ATPase beta subunit deficient mice.
Bakkelund KE, Waldum HL, Nordrum IS, Hauso ?, Fossmark R
Scandinavian journal of gastroenterology45(9)1042-7(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study on N-terminally truncated beta-variants of the gastric H,K-ATPase provides direct evidence that the beta-N terminus assists in E2P state stabilization and that this is critical for the transport efficiency of the enzyme under in vivo conditions.
Title: E2P state stabilization by the N-terminal tail of the H,K-ATPase beta-subunit is critical for efficient proton pumping under in vivo conditions.

The substitution of 5 amino acids in the low affinity ouabain binding site of non-gastric proton-potassium exchanging ATPase in order to increase ouabain binding affinity is reported.
Title: Conversion of the low affinity ouabain-binding site of non-gastric H,K-ATPase into a high affinity binding site by substitution of only five amino acids.

Non-gastric H,K-ATPase is oligomycin-sensitive and can function as H+,NH4(+)-ATPase.
Title: The non-gastric H,K-ATPase is oligomycin-sensitive and can function as an H+,NH4(+)-ATPase.

The following Atp4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42381	NM_012510.2 Latest version!	Rattus norvegicus ATPase H+/K+ transporting subunit beta (Atp4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp4b ( NM_012510.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036642.2 Rattus norvegicus ATPase H+/K+ transporting subunit beta (Atp4b), mRNA.

Price & Availability↑

CloneID	ORa42381
Clone ID Related Accession (Same CDS sequence)	NM_012510.2
Accession Version	NM_012510.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	potassium-transporting ATPase subunit beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M35535.1. On Jun 9, 2003 this sequence version replaced NM_012510.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M35535.1, M55655.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689601, SAMN02642675 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCCC TGCAGGAGAA GAAGTCATGC AGCCAGCGCA TGGCCGAATT CCGGCAATAC 
TGTTGGAACC CGGACACTGG GCAGATGCTG GGCCGCACCC CAGCCCGGTG GGTGTGGATC 
AGCCTGTACT ATGCAGCTTT CTACGTGGTC ATGACTGGGC TCTTTGCCTT GTGCATCTAT 
GTGCTGATGC AGACCATTGA TCCCTACACC CCCGACTACC AGGACCAGTT AAAGTCACCG 
GGGGTAACCT TGAGACCGGA TGTGTATGGG GAAAGAGGGC TGCAGATTTC CTACAACATC 
TCTGAAAACA GCTCCTGGGC TGGCCTCACA CACACCCTCC ACAGCTTCTT AGCGGGCTAC 
ACCCCAGCAT CCCAGCAGGA CAGCATCAAC TGTTCGTCTG AAAAGTACTT CTTCCAGGAG 
ACCTTTTCTG CTCCGAACCA TACCAAGTTC TCCTGCAAGT TCACGGCGGA CATGCTACAG 
AATTGCTCAG GCCTGGTGGA CCCCAGTTTC GGCTTTGAGG AGGGAAAGCC CTGCTTCATT 
ATTAAAATGA ACAGGATTGT CAAGTTCCTG CCCAGCAACA ACACGGCTCC CCGAGTGGAC 
TGCACCTTCC AGGATGACCC CCAAAAGCCC CGGAAGGACA TTGAACCCCT GCAGGTCCAG 
TACTATCCCC CCAATGGTAC CTTCAGTCTC CACTACTTCC CCTACTACGG CAAGAAAGCA 
CAGCCCCACT ACAGCAACCC TCTGGTGGCG GCAAAGTTCC TCAACGTCCC CAAAAACACG 
CAAGTCCTCA TCGTGTGCAA GATCATGGCG GACCACGTGA CCTTCGACAA CCCCCACGAC 
CCCTATGAAG GGAAGGTGGA GTTCAAGCTC ACAATACAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036642.2
CDS	176..1060
Translation	MAALQEKKSCSQRMAEFRQYCWNPDTGQMLGRTPARWVWISLYYAAFYVVMTGLFALCIYVLMQTIDPYTPDYQDQLKSPGVTLRPDVYGERGLQISYNISENSSWAGLTHTLHSFLAGYTPASQQDSINCSSEKYFFQETFSAPNHTKFSCKFTADMLQNCSGLVDPSFGFEEGKPCFIIKMNRIVKFLPSNNTAPRVDCTFQDDPQKPRKDIEPLQVQYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKFLNVPKNTQVLIVCKIMADHVTFDNPHDPYEGKVEFKLTIQK

Target ORF information:

RefSeq Version	NM_012510.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase H+/K+ transporting subunit beta (Atp4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012510.2

ORF Insert Sequence:

ATGGCAGCCC TGCAGGAGAA GAAGTCATGC AGCCAGCGCA TGGCCGAATT CCGGCAATAC 
TGTTGGAACC CGGACACTGG GCAGATGCTG GGCCGCACCC CAGCCCGGTG GGTGTGGATC 
AGCCTGTACT ATGCAGCTTT CTACGTGGTC ATGACTGGGC TCTTTGCCTT GTGCATCTAT 
GTGCTGATGC AGACCATTGA TCCCTACACC CCCGACTACC AGGACCAGTT AAAGTCACCG 
GGGGTAACCT TGAGACCGGA TGTGTATGGG GAAAGAGGGC TGCAGATTTC CTACAACATC 
TCTGAAAACA GCTCCTGGGC TGGCCTCACA CACACCCTCC ACAGCTTCTT AGCGGGCTAC 
ACCCCAGCAT CCCAGCAGGA CAGCATCAAC TGTTCGTCTG AAAAGTACTT CTTCCAGGAG 
ACCTTTTCTG CTCCGAACCA TACCAAGTTC TCCTGCAAGT TCACGGCGGA CATGCTACAG 
AATTGCTCAG GCCTGGTGGA CCCCAGTTTC GGCTTTGAGG AGGGAAAGCC CTGCTTCATT 
ATTAAAATGA ACAGGATTGT CAAGTTCCTG CCCAGCAACA ACACGGCTCC CCGAGTGGAC 
TGCACCTTCC AGGATGACCC CCAAAAGCCC CGGAAGGACA TTGAACCCCT GCAGGTCCAG 
TACTATCCCC CCAATGGTAC CTTCAGTCTC CACTACTTCC CCTACTACGG CAAGAAAGCA 
CAGCCCCACT ACAGCAACCC TCTGGTGGCG GCAAAGTTCC TCAACGTCCC CAAAAACACG 
CAAGTCCTCA TCGTGTGCAA GATCATGGCG GACCACGTGA CCTTCGACAA CCCCCACGAC 
CCCTATGAAG GGAAGGTGGA GTTCAAGCTC ACAATACAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of the Histamine 1 Receptor Antagonist Cetirizine on the Osteoporotic Phenotype in H(+) /K(+) ATPase Beta Subunit KO Mice.
Journal of cellular biochemistry117(9)2089-96(2016 09)
Aasar?d KM,Stunes AK,Mosti MP,Ramezanzadehkoldeh M,Viggaklev BI,Reseland JE,Skallerud BH,Fossmark R,Syversen U

Remodeling of the residual gastric mucosa after roux-en-y gastric bypass or vertical sleeve gastrectomy in diet-induced obese rats.
PloS one10(3)e0121414(2015)
Arapis K,Cavin JB,Gillard L,Cluzeaud F,Lett?ron P,Ducroc R,Le Beyec J,Hourseau M,Couvelard A,Marmuse JP,Le Gall M,Bado A

Intragenic DNA methylation concomitant with repression of ATP4B and ATP4A gene expression in gastric cancer is a potential serum biomarker.
Asian Pacific journal of cancer prevention : APJCP13(11)5563-8(2012)
Raja UM,Gopal G,Rajkumar T

Long-term gastric changes in achlorhydric H(+)/K(+)-ATPase beta subunit deficient mice.
Scandinavian journal of gastroenterology45(9)1042-7(2010 Sep)
Bakkelund KE,Waldum HL,Nordrum IS,Hauso ?,Fossmark R

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Atp4b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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