ATP4B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP4B
Entrez Gene ID	496
Full Name	ATPase H+/K+ transporting beta subunit
Synonyms	ATP6B
General protein information	Preferred Names ATPase H+/K+ transporting beta subunit Names potassium-transporting ATPase subunit beta ATPase, H+/K+ exchanging, beta polypeptide ATPase, H+/K+ transporting, beta polypeptide gastric H(+)/K(+) ATPase subunit beta gastric H+/K+ ATPase beta subunit gastric hydrogen-potassium ATPase, beta potassium-transporting ATPase beta chain proton pump beta chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q34
Summary	The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008].
Disorder MIM:	137217

mRNA and Protein(s)

mRNA	Protein	Name
NM_000705.4	NP_000696.1	potassium-transporting ATPase subunit beta
NM_000705.3	NP_000696.1	potassium-transporting ATPase subunit beta

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ATP4B	NP_001193069.1
Gallus gallus (chicken)	ATP4B	NP_989749.1
Mus musculus (house mouse)	Atp4b	NP_033854.1
Xenopus tropicalis (tropical clawed frog)	atp4b	NP_001005806.1
Homo sapiens (human)	ATP4B	NP_000696.1
Rattus norvegicus (Norway rat)	Atp4b	NP_036642.2
Pan troglodytes (chimpanzee)	ATP4B	XP_001146058.1
Canis lupus familiaris (dog)	ATP4B	NP_001003328.1
	ATP4B	XP_001088163.1

Gene Ontology
Bibliography

Related articles in PubMed

Benzimidazole derivative, BMT-1, induces apoptosis in multiple myeloma cells via a mitochondrial-mediated pathway involving H+/K+-ATPase inhibition.
Yang T, Li MH, Liu J, Huang N, Li N, Liu SN, Liu Y, Zhang T, Zou Q, Li H
Oncology reports31(6)2743-50(2014 Jun)

Intragenic DNA methylation concomitant with repression of ATP4B and ATP4A gene expression in gastric cancer is a potential serum biomarker.
Raja UM, Gopal G, Rajkumar T
Asian Pacific journal of cancer prevention : APJCP13(11)5563-8(2012)

A C-terminal lobe of the beta subunit of Na,K-ATPase and H,K-ATPase resembles cell adhesion molecules.
Bab-Dinitz E, Albeck S, Peleg Y, Brumfeld V, Gottschalk KE, Karlish SJ
Biochemistry48(36)8684-91(2009 Sep)

Pharmacological effects of lipid-lowering drugs recapitulate with a larger amplitude the phenotypic effects of common variants within their target genes.
Knouff CW, Lim N, Song K, Yuan X, Walker MC, Townsend R, Waeber G, Matthews PM, Vollenweider P, Waterworth DM, Mooser V
Pharmacogenetics and genomics18(12)1051-7(2008 Dec)

Transcriptome analysis of human gastric cancer.
Oh JH, Yang JO, Hahn Y, Kim MR, Byun SS, Jeon YJ, Kim JM, Song KS, Noh SM, Kim S, Yoo HS, Kim YS, Kim NS
Mammalian genome : official journal of the International Mammalian Genome Society16(12)942-54(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings demonstrated that a decrease in pHi, caused by H+/K+-ATPase inhibition induced by BMT-1, triggered the dysfunction of the mitochondria resulting in the apoptosis of MM cells
Title: Benzimidazole derivative, BMT-1, induces apoptosis in multiple myeloma cells via a mitochondrial-mediated pathway involving H+/K+-ATPase inhibition.

Downregulation of ATP4B gene is associated with gastric cancer.
Title: Intragenic DNA methylation concomitant with repression of ATP4B and ATP4A gene expression in gastric cancer is a potential serum biomarker.

Use of fold recognition methods enables the prediction that a C-terminal domain of the beta subunits of Na,K-ATPase and H,K-ATPase has an immunoglobulin-like fold, which resembles cell adhesion molecules.
Title: A C-terminal lobe of the beta subunit of Na,K-ATPase and H,K-ATPase resembles cell adhesion molecules.

Observational study of gene-disease association. (HuGE Navigator)
Title: Pharmacological effects of lipid-lowering drugs recapitulate with a larger amplitude the phenotypic effects of common variants within their target genes.

Thus prolonged exhaustive exercise impaired each of the maximal in vitro Na+-K+-ATPase activity, Ca2+ release, and Ca2+ uptake rates.
Title: Prolonged exercise to fatigue in humans impairs skeletal muscle Na+-K+-ATPase activity, sarcoplasmic reticulum Ca2+ release, and Ca2+ uptake.

The following ATP4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02583	NM_000705.4 Latest version!	Homo sapiens ATPase H+/K+ transporting subunit beta (ATP4B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu02583	NM_000705.3	Homo sapiens ATPase H+/K+ transporting subunit beta (ATP4B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP4B ( NM_000705.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000696.1 Homo sapiens ATPase H+/K+ transporting subunit beta (ATP4B), mRNA.

Price & Availability↑

CloneID	OHu02583
Clone ID Related Accession (Same CDS sequence)	NM_000705.4 , NM_000705.3
Accession Version	NM_000705.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	potassium-transporting ATPase subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA964061.1, BC029059.1, BI764514.1 and BX100065.1. On May 17, 2019 this sequence version replaced NM_000705.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC029059.1, M75110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000335288.5/ ENSP00000334216.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTC TGCAGGAGAA GAAGACGTGT GGCCAGCGCA TGGAGGAGTT CCAGCGTTAC 
TGCTGGAACC CGGACACGGG GCAGATGCTG GGCCGCACCC TGTCCCGGTG GGTGTGGATC 
AGCCTGTACT ACGTGGCCTT CTACGTGGTG ATGACTGGGC TCTTCGCCCT GTGCCTCTAT 
GTGCTGATGC AGACAGTGGA CCCGTACACA CCGGACTACC AAGACCAGCT ACGGTCACCA 
GGGGTAACCT TAAGGCCGGA TGTTTACGGG GAGAAAGGCC TGGAAATTGT CTACAACGTC 
TCTGATAACA GAACCTGGGC AGACCTCACA CAGACTCTCC ACGCCTTCCT AGCAGGCTAC 
TCTCCAGCAG CCCAGGAGGA CAGCATCAAC TGCACCTCCG AGCAGTACTT CTTCCAGGAG 
AGTTTCCGCG CTCCCAACCA CACCAAGTTC TCCTGCAAGT TCACGGCAGA TATGCTGCAG 
AACTGCTCAG GCCTGGCGGA TCCCAACTTC GGCTTTGAAG AAGGAAAGCC ATGTTTTATT 
ATTAAAATGA ACAGGATCGT CAAGTTCCTC CCCAGCAACG GCTCGGCCCC CAGAGTGGAC 
TGCGCCTTCC TGGACCAGCC CCGCGAGCTC GGCCAGCCGC TGCAGGTCAA GTACTACCCT 
CCCAACGGCA CCTTCAGTCT GCACTACTTC CCTTATTACG GGAAGAAAGC CCAGCCCCAC 
TACAGCAACC CCCTGGTGGC AGCGAAGCTC CTCAACATCC CCAGGAACGC TGAGGTCGCC 
ATCGTGTGCA AGGTCATGGC GGAGCACGTG ACCTTCAACA ATCCCCACGA CCCGTATGAA 
GGGAAAGTGG AGTTCAAACT CAAGATTGAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000696.1
CDS	55..930
Translation	MAALQEKKTCGQRMEEFQRYCWNPDTGQMLGRTLSRWVWISLYYVAFYVVMTGLFALCLYVLMQTVDPYTPDYQDQLRSPGVTLRPDVYGEKGLEIVYNVSDNRTWADLTQTLHAFLAGYSPAAQEDSINCTSEQYFFQESFRAPNHTKFSCKFTADMLQNCSGLADPNFGFEEGKPCFIIKMNRIVKFLPSNGSAPRVDCAFLDQPRELGQPLQVKYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKLLNIPRNAEVAIVCKVMAEHVTFNNPHDPYEGKVEFKLKIEK

Target ORF information:

RefSeq Version	NM_000705.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+/K+ transporting subunit beta (ATP4B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000705.4

ORF Insert Sequence:

ATGGCGGCTC TGCAGGAGAA GAAGACGTGT GGCCAGCGCA TGGAGGAGTT CCAGCGTTAC 
TGCTGGAACC CGGACACGGG GCAGATGCTG GGCCGCACCC TGTCCCGGTG GGTGTGGATC 
AGCCTGTACT ACGTGGCCTT CTACGTGGTG ATGACTGGGC TCTTCGCCCT GTGCCTCTAT 
GTGCTGATGC AGACAGTGGA CCCGTACACA CCGGACTACC AAGACCAGCT ACGGTCACCA 
GGGGTAACCT TAAGGCCGGA TGTTTACGGG GAGAAAGGCC TGGAAATTGT CTACAACGTC 
TCTGATAACA GAACCTGGGC AGACCTCACA CAGACTCTCC ACGCCTTCCT AGCAGGCTAC 
TCTCCAGCAG CCCAGGAGGA CAGCATCAAC TGCACCTCCG AGCAGTACTT CTTCCAGGAG 
AGTTTCCGCG CTCCCAACCA CACCAAGTTC TCCTGCAAGT TCACGGCAGA TATGCTGCAG 
AACTGCTCAG GCCTGGCGGA TCCCAACTTC GGCTTTGAAG AAGGAAAGCC ATGTTTTATT 
ATTAAAATGA ACAGGATCGT CAAGTTCCTC CCCAGCAACG GCTCGGCCCC CAGAGTGGAC 
TGCGCCTTCC TGGACCAGCC CCGCGAGCTC GGCCAGCCGC TGCAGGTCAA GTACTACCCT 
CCCAACGGCA CCTTCAGTCT GCACTACTTC CCTTATTACG GGAAGAAAGC CCAGCCCCAC 
TACAGCAACC CCCTGGTGGC AGCGAAGCTC CTCAACATCC CCAGGAACGC TGAGGTCGCC 
ATCGTGTGCA AGGTCATGGC GGAGCACGTG ACCTTCAACA ATCCCCACGA CCCGTATGAA 
GGGAAAGTGG AGTTCAAACT CAAGATTGAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP4B ( NM_000705.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000696.1 Homo sapiens ATPase H+/K+ transporting subunit beta (ATP4B), mRNA.

Price & Availability↑

CloneID	OHu02583
Clone ID Related Accession (Same CDS sequence)	NM_000705.4 , NM_000705.3
Accession Version	NM_000705.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	potassium-transporting ATPase subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA964061.1, BC029059.1, BI764514.1 and BX100065.1. On Jun 3, 2010 this sequence version replaced NM_000705.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC029059.1, M75110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTC TGCAGGAGAA GAAGACGTGT GGCCAGCGCA TGGAGGAGTT CCAGCGTTAC 
TGCTGGAACC CGGACACGGG GCAGATGCTG GGCCGCACCC TGTCCCGGTG GGTGTGGATC 
AGCCTGTACT ACGTGGCCTT CTACGTGGTG ATGACTGGGC TCTTCGCCCT GTGCCTCTAT 
GTGCTGATGC AGACAGTGGA CCCGTACACA CCGGACTACC AAGACCAGCT ACGGTCACCA 
GGGGTAACCT TAAGGCCGGA TGTTTACGGG GAGAAAGGCC TGGAAATTGT CTACAACGTC 
TCTGATAACA GAACCTGGGC AGACCTCACA CAGACTCTCC ACGCCTTCCT AGCAGGCTAC 
TCTCCAGCAG CCCAGGAGGA CAGCATCAAC TGCACCTCCG AGCAGTACTT CTTCCAGGAG 
AGTTTCCGCG CTCCCAACCA CACCAAGTTC TCCTGCAAGT TCACGGCAGA TATGCTGCAG 
AACTGCTCAG GCCTGGCGGA TCCCAACTTC GGCTTTGAAG AAGGAAAGCC ATGTTTTATT 
ATTAAAATGA ACAGGATCGT CAAGTTCCTC CCCAGCAACG GCTCGGCCCC CAGAGTGGAC 
TGCGCCTTCC TGGACCAGCC CCGCGAGCTC GGCCAGCCGC TGCAGGTCAA GTACTACCCT 
CCCAACGGCA CCTTCAGTCT GCACTACTTC CCTTATTACG GGAAGAAAGC CCAGCCCCAC 
TACAGCAACC CCCTGGTGGC AGCGAAGCTC CTCAACATCC CCAGGAACGC TGAGGTCGCC 
ATCGTGTGCA AGGTCATGGC GGAGCACGTG ACCTTCAACA ATCCCCACGA CCCGTATGAA 
GGGAAAGTGG AGTTCAAACT CAAGATTGAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000696.1
CDS	55..930
Translation	MAALQEKKTCGQRMEEFQRYCWNPDTGQMLGRTLSRWVWISLYYVAFYVVMTGLFALCLYVLMQTVDPYTPDYQDQLRSPGVTLRPDVYGEKGLEIVYNVSDNRTWADLTQTLHAFLAGYSPAAQEDSINCTSEQYFFQESFRAPNHTKFSCKFTADMLQNCSGLADPNFGFEEGKPCFIIKMNRIVKFLPSNGSAPRVDCAFLDQPRELGQPLQVKYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKLLNIPRNAEVAIVCKVMAEHVTFNNPHDPYEGKVEFKLKIEK

Target ORF information:

RefSeq Version	NM_000705.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+/K+ transporting subunit beta (ATP4B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000705.3

ORF Insert Sequence:

ATGGCGGCTC TGCAGGAGAA GAAGACGTGT GGCCAGCGCA TGGAGGAGTT CCAGCGTTAC 
TGCTGGAACC CGGACACGGG GCAGATGCTG GGCCGCACCC TGTCCCGGTG GGTGTGGATC 
AGCCTGTACT ACGTGGCCTT CTACGTGGTG ATGACTGGGC TCTTCGCCCT GTGCCTCTAT 
GTGCTGATGC AGACAGTGGA CCCGTACACA CCGGACTACC AAGACCAGCT ACGGTCACCA 
GGGGTAACCT TAAGGCCGGA TGTTTACGGG GAGAAAGGCC TGGAAATTGT CTACAACGTC 
TCTGATAACA GAACCTGGGC AGACCTCACA CAGACTCTCC ACGCCTTCCT AGCAGGCTAC 
TCTCCAGCAG CCCAGGAGGA CAGCATCAAC TGCACCTCCG AGCAGTACTT CTTCCAGGAG 
AGTTTCCGCG CTCCCAACCA CACCAAGTTC TCCTGCAAGT TCACGGCAGA TATGCTGCAG 
AACTGCTCAG GCCTGGCGGA TCCCAACTTC GGCTTTGAAG AAGGAAAGCC ATGTTTTATT 
ATTAAAATGA ACAGGATCGT CAAGTTCCTC CCCAGCAACG GCTCGGCCCC CAGAGTGGAC 
TGCGCCTTCC TGGACCAGCC CCGCGAGCTC GGCCAGCCGC TGCAGGTCAA GTACTACCCT 
CCCAACGGCA CCTTCAGTCT GCACTACTTC CCTTATTACG GGAAGAAAGC CCAGCCCCAC 
TACAGCAACC CCCTGGTGGC AGCGAAGCTC CTCAACATCC CCAGGAACGC TGAGGTCGCC 
ATCGTGTGCA AGGTCATGGC GGAGCACGTG ACCTTCAACA ATCCCCACGA CCCGTATGAA 
GGGAAAGTGG AGTTCAAACT CAAGATTGAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Benzimidazole derivative, BMT-1, induces apoptosis in multiple myeloma cells via a mitochondrial-mediated pathway involving H+/K+-ATPase inhibition.
Oncology reports31(6)2743-50(2014 Jun)
Yang T,Li MH,Liu J,Huang N,Li N,Liu SN,Liu Y,Zhang T,Zou Q,Li H

Intragenic DNA methylation concomitant with repression of ATP4B and ATP4A gene expression in gastric cancer is a potential serum biomarker.
Asian Pacific journal of cancer prevention : APJCP13(11)5563-8(2012)
Raja UM,Gopal G,Rajkumar T

A C-terminal lobe of the beta subunit of Na,K-ATPase and H,K-ATPase resembles cell adhesion molecules.
Biochemistry48(36)8684-91(2009 Sep)
Bab-Dinitz E,Albeck S,Peleg Y,Brumfeld V,Gottschalk KE,Karlish SJ

Pharmacological effects of lipid-lowering drugs recapitulate with a larger amplitude the phenotypic effects of common variants within their target genes.
Pharmacogenetics and genomics18(12)1051-7(2008 Dec)
Knouff CW,Lim N,Song K,Yuan X,Walker MC,Townsend R,Waeber G,Matthews PM,Vollenweider P,Waterworth DM,Mooser V

Transcriptome analysis of human gastric cancer.
Mammalian genome : official journal of the International Mammalian Genome Society16(12)942-54(2005 Dec)
Oh JH,Yang JO,Hahn Y,Kim MR,Byun SS,Jeon YJ,Kim JM,Song KS,Noh SM,Kim S,Yoo HS,Kim YS,Kim NS

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ATP4B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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