Fap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fap
Entrez Gene ID	192203
Full Name	fibroblast activation protein, alpha
General protein information	Preferred Names fibroblast activation protein, alpha Names prolyl endopeptidase FAP seprase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q21
Summary	plays a role in liver disease, T cell costimulation, chemokine biology, type II diabetes, and tumor biology [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138850.1	NP_620205.1	prolyl endopeptidase FAP

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Fap	NP_032012.1
Gallus gallus (chicken)	FAP	NP_001180568.1
Pan troglodytes (chimpanzee)	FAP	XP_515860.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011584	XP_309924.4
Xenopus tropicalis (tropical clawed frog)	fap	NP_001107145.1
Drosophila melanogaster (fruit fly)	ome	NP_001097606.1
	FAP	XP_001096858.1
Bos taurus (cattle)	FAP	NP_001091470.1
Rattus norvegicus (Norway rat)	Fap	NP_620205.1
Magnaporthe oryzae (rice blast fungus)	MGG_05989	XP_003711833.1
Homo sapiens (human)	FAP	NP_004451.2
Canis lupus familiaris (dog)	FAP	XP_005640309.1

Gene Ontology
Bibliography

Related articles in PubMed

Fibroblast activation protein alpha expression identifies activated fibroblasts after myocardial infarction.
Tillmanns J, Hoffmann D, Habbaba Y, Schmitto JD, Sedding D, Fraccarollo D, Galuppo P, Bauersachs J
Journal of molecular and cellular cardiology87194-203(2015 Oct)

Regulation of mesenchymal-to-epithelial transition by PARAXIS during somitogenesis.
Rowton M, Ramos P, Anderson DM, Rhee JM, Cunliffe HE, Rawls A
Developmental dynamics : an official publication of the American Association of Anatomists242(11)1332-44(2013 Nov)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

Expression of fibroblast activation proteins in corneal stromal neovascularization.
Wang T, Shi W
Current eye research34(2)112-7(2009 Feb)

The effect of a single nucleotide polymorphism on human alpha 2-antiplasmin activity.
Christiansen VJ, Jackson KW, Lee KN, McKee PA
Blood109(12)5286-92(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this study, we show for the first time the expression of FAP in activated fibroblasts after MI and its activation by TGFbeta1. Effects of FAP on fibroblast migration and gelatinolytic activity indicate a potential role in cardiac wound healing
Title: Fibroblast activation protein alpha expression identifies activated fibroblasts after myocardial infarction.

Factors in corneal stroma may change when corneal neovascularization occurs. The stromal keratocytes can express FAP(+) cells surrounding the endothelium of angiogenesis.
Title: Expression of fibroblast activation proteins in corneal stromal neovascularization.

The following Fap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01734	NM_138850.1 Latest version!	Rattus norvegicus fibroblast activation protein, alpha (Fap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$447.30 $639.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fap ( NM_138850.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620205.1 Rattus norvegicus fibroblast activation protein, alpha (Fap), mRNA.

Price & Availability↑

CloneID	ORa01734
Clone ID Related Accession (Same CDS sequence)	NM_138850.1
Accession Version	NM_138850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	prolyl endopeptidase FAP
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF493782.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF493782.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAGACAT GGCTGAAAAC CGTCTTTGGA GTCACTACTT TGGTTGCGCT TGCTCTAGTG 
GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAGCC CTGAAGGCAA CACAGGAAGA 
TCTCTTACCC TGAAGGATAT TTTAAATGGA ACATTCTCCT ATAAAACATA TTTTCCAAAC 
TGGATTTCAG AGCAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT CTTTTATAAT 
ATTGAAACAA GAGAATCCTA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT 
ACAGACTATG GCCTGTCACC TGATCGGCAG TTTATATATC TTGAAAGTGA TTATTCAAAG 
CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT 
GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTGT GCTGGTCTCC CGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATT TATTTAAAAC AAAGACCAGG AGATCCACCT 
TTTCAAATAA CTTACACTGG AAGAGAAAAT AGAATATTTA ATGGAATTCC AGACTGGGTT 
TATGAAGAGG AAATGCTTGC TACAAAATAT GCCCTTTGGT GGTCCCCAGA TGGAAAATAT 
TTGGCATATG TAGAATTTAA TGATTCGGAC ATACCAATTA TTGCCTACTC GTATTATGGC 
GATGGACAGT ATCCTAGAAC TATAAATATT CCATACCCGA AGGCCGGAGC TAAGAATCCC 
ATTGTTCGTG TCTTTATTGT TGACACTATC TACCCACACC ATGTGGGTCC TATAGAAGTA 
CCAGTTCCAG AGATGATAGC CTCGAGTGAC TACTATTTCA CCTGGCTCAC ATGGGTGACC 
AACGAACGAG TATGTTTGCA GTGGCTAAAG AGAGTGCAGA ATGTCTCAGT CTTGTCTATA 
TGTGATTTCA GAGAAGACTG GCATGCGTGG GATTGTCCAA AGAACCAGGA GCATATTGAA 
GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCCA CACCAGCTTT TAGCCAGGAT 
GCCGCTTCAT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC 
AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA 
TTCCGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA 
AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGTAAGAA GTGTGTTACT 
TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAA 
TACTATGCAC TCATCTGCTA CGGCCCTGGC CTCCCGATTT CCACCCTCCA TGATGGCCGC 
ACAGACCAAG AAATACAAGT ATTAGAAGAG AACAAAGAAC TGGAGAACGC TCTGAGAAAC 
ATCCAGCTGC CTGCAGTGGA AATTAAGAAG CTTGAAGACG GTGGAATGAC TTTCTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTCTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGCCA GAGTGTAAAG TCTGTATTCT CTGTTAATTG GATAACTTAT 
CTTGCAAGTA AAGAGGGGAT AGTCGTTGCC TTGGTAGATG GCCGGGGCAC TGCTTTCCAA 
GGTGACAAAT TCCTGCATGC CGTGTATCGA AAGCTGGGTG TGTATGAAGT TGAGGACCAG 
CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGGAAG AATAGCCATA 
TGGGGATGGT CCTACGGAGG TTATGTTTCA TCCCTAGCCC TTGCGTCCGG AACTGGCCTT 
TTCAAATGTG GTATAGCAGT GGCTCCAGTC TCCAGCTGGG AATACTACGC CTCTATCTAC 
ACGGAGAGAT TCATGGGCCT CCCAACAAAG GATGATAATC TTGAACACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAACGCA 
CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGCATATC ATCTGGGCGC 
TCCCAGAACC ACTTATACAC TCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620205.1
CDS	8..2293
Translation	MKTWLKTVFGVTTLVALALVVICIVLRPSRVYSPEGNTGRSLTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFIYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKYLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPIVRVFIVDTIYPHHVGPIEVPVPEMIASSDYYFTWLTWVTNERVCLQWLKRVQNVSVLSICDFREDWHAWDCPKNQEHIEESRTGWAGGFFVSTPAFSQDAASYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALICYGPGLPISTLHDGRTDQEIQVLEENKELENALRNIQLPAVEIKKLEDGGMTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFSVNWITYLASKEGIVVALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEGRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Target ORF information:

RefSeq Version	NM_138850.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fibroblast activation protein, alpha (Fap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138850.1

ORF Insert Sequence:

ATGAAGACAT GGCTGAAAAC CGTCTTTGGA GTCACTACTT TGGTTGCGCT TGCTCTAGTG 
GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAGCC CTGAAGGCAA CACAGGAAGA 
TCTCTTACCC TGAAGGATAT TTTAAATGGA ACATTCTCCT ATAAAACATA TTTTCCAAAC 
TGGATTTCAG AGCAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT CTTTTATAAT 
ATTGAAACAA GAGAATCCTA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT 
ACAGACTATG GCCTGTCACC TGATCGGCAG TTTATATATC TTGAAAGTGA TTATTCAAAG 
CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT 
GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTGT GCTGGTCTCC CGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATT TATTTAAAAC AAAGACCAGG AGATCCACCT 
TTTCAAATAA CTTACACTGG AAGAGAAAAT AGAATATTTA ATGGAATTCC AGACTGGGTT 
TATGAAGAGG AAATGCTTGC TACAAAATAT GCCCTTTGGT GGTCCCCAGA TGGAAAATAT 
TTGGCATATG TAGAATTTAA TGATTCGGAC ATACCAATTA TTGCCTACTC GTATTATGGC 
GATGGACAGT ATCCTAGAAC TATAAATATT CCATACCCGA AGGCCGGAGC TAAGAATCCC 
ATTGTTCGTG TCTTTATTGT TGACACTATC TACCCACACC ATGTGGGTCC TATAGAAGTA 
CCAGTTCCAG AGATGATAGC CTCGAGTGAC TACTATTTCA CCTGGCTCAC ATGGGTGACC 
AACGAACGAG TATGTTTGCA GTGGCTAAAG AGAGTGCAGA ATGTCTCAGT CTTGTCTATA 
TGTGATTTCA GAGAAGACTG GCATGCGTGG GATTGTCCAA AGAACCAGGA GCATATTGAA 
GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCCA CACCAGCTTT TAGCCAGGAT 
GCCGCTTCAT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC 
AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA 
TTCCGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA 
AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGTAAGAA GTGTGTTACT 
TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAA 
TACTATGCAC TCATCTGCTA CGGCCCTGGC CTCCCGATTT CCACCCTCCA TGATGGCCGC 
ACAGACCAAG AAATACAAGT ATTAGAAGAG AACAAAGAAC TGGAGAACGC TCTGAGAAAC 
ATCCAGCTGC CTGCAGTGGA AATTAAGAAG CTTGAAGACG GTGGAATGAC TTTCTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTCTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGCCA GAGTGTAAAG TCTGTATTCT CTGTTAATTG GATAACTTAT 
CTTGCAAGTA AAGAGGGGAT AGTCGTTGCC TTGGTAGATG GCCGGGGCAC TGCTTTCCAA 
GGTGACAAAT TCCTGCATGC CGTGTATCGA AAGCTGGGTG TGTATGAAGT TGAGGACCAG 
CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGGAAG AATAGCCATA 
TGGGGATGGT CCTACGGAGG TTATGTTTCA TCCCTAGCCC TTGCGTCCGG AACTGGCCTT 
TTCAAATGTG GTATAGCAGT GGCTCCAGTC TCCAGCTGGG AATACTACGC CTCTATCTAC 
ACGGAGAGAT TCATGGGCCT CCCAACAAAG GATGATAATC TTGAACACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAACGCA 
CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGCATATC ATCTGGGCGC 
TCCCAGAACC ACTTATACAC TCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fibroblast activation protein alpha expression identifies activated fibroblasts after myocardial infarction.
Journal of molecular and cellular cardiology87194-203(2015 Oct)
Tillmanns J,Hoffmann D,Habbaba Y,Schmitto JD,Sedding D,Fraccarollo D,Galuppo P,Bauersachs J

Regulation of mesenchymal-to-epithelial transition by PARAXIS during somitogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists242(11)1332-44(2013 Nov)
Rowton M,Ramos P,Anderson DM,Rhee JM,Cunliffe HE,Rawls A

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

Expression of fibroblast activation proteins in corneal stromal neovascularization.
Current eye research34(2)112-7(2009 Feb)
Wang T,Shi W

The effect of a single nucleotide polymorphism on human alpha 2-antiplasmin activity.
Blood109(12)5286-92(2007 Jun)
Christiansen VJ,Jackson KW,Lee KN,McKee PA