Nlgn3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlgn3
Entrez Gene ID	171297
Full Name	neuroligin 3
General protein information	Preferred Names neuroligin 3 Names neuroligin-3 gliotactin homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq22
Summary	neuronal cell surface protein that binds neurexins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006257054.3	XP_006257116.1	neuroligin-3 isoform X2
NM_134336.2	NP_599163.2	neuroligin-3 precursor
XM_008773251.2	XP_008771473.1	neuroligin-3 isoform X1
XM_006257055.3	XP_006257117.1	neuroligin-3 isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nlgn3	NP_599163.2
Drosophila melanogaster (fruit fly)	CG34139	NP_001036730.2
Canis lupus familiaris (dog)	NLGN3	XP_005641518.1
Mus musculus (house mouse)	Nlgn3	NP_766520.2
Xenopus tropicalis (tropical clawed frog)	nlgn3	NP_001165298.1
Homo sapiens (human)	NLGN3	NP_851820.1
Pan troglodytes (chimpanzee)	NLGN3	XP_529033.2
Gallus gallus (chicken)	NLGN3	NP_001166992.1
Danio rerio (zebrafish)	nlgn3a	NP_001159804.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003115	XP_312799.5
Bos taurus (cattle)	NLGN3	NP_001068972.1

Gene Ontology
Bibliography

Related articles in PubMed

Distinct roles for extracellular and intracellular domains in neuroligin function at inhibitory synapses.
Nguyen QA, Horn ME, Nicoll RA
eLife5(2016 11)

Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system.
Ulbrich L, Favaloro FL, Trobiani L, Marchetti V, Patel V, Pascucci T, Comoletti D, Marciniak SJ, De Jaco A
The Biochemical journal473(4)423-34(2016 Feb)

In vivo clonal overexpression of neuroligin 3 and neuroligin 2 in neurons of the rat cerebral cortex: Differential effects on GABAergic synapses and neuronal migration.
Fekete CD, Chiou TT, Miralles CP, Harris RS, Fiondella CG, Loturco JJ, De Blas AL
The Journal of comparative neurology523(9)1359-78(2015 Jun)

Fmr1 and Nlgn3 knockout rats: novel tools for investigating autism spectrum disorders.
Hamilton SM, Green JR, Veeraragavan S, Yuva L, McCoy A, Wu Y, Warren J, Little L, Ji D, Cui X, Weinstein E, Paylor R
Behavioral neuroscience128(2)103-9(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

e found that NLGN3 function at inhibitory synapses in rat CA1 depends on the presence of NLGN2 and identified a domain in the extracellular region that accounted for this functional difference between NLGN2 and 3 specifically at inhibitory synapses.
Title: Distinct roles for extracellular and intracellular domains in neuroligin function at inhibitory synapses.

show that up-regulation of BiP (immunoglobulin heavy-chain-binding protein) and CHOP [C/EBP (CCAAT/enhancer-binding protein)-homologous protein] was induced by both mutant proteins but not by wild-type neuroligin3
Title: Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system.

Studied the effect of clonal overexpression of NL3 and NL2 in neurons of the rat cerebral cortex in vivo after in utero electroporation, found that overexpression of NL3 has strong effects on GABAergic synapses and neuronal radial migration
Title: In vivo clonal overexpression of neuroligin 3 and neuroligin 2 in neurons of the rat cerebral cortex: Differential effects on GABAergic synapses and neuronal migration.

Data from studies using cross-linking reagents suggest that neuroligins in neurons cultured from embryonic hippocampus, striatum, or cerebellum form constitutive dimers, including homodimers and, most notably, neuroligin 1/3 heteromers.
Title: Homodimerization and isoform-specific heterodimerization of neuroligins.

On a wild-type background, overexpression of a full-length version of NLGN3 yielded an increase in AMPA receptor (AMPAR)-evoked currents.
Title: Functional dependence of neuroligin on a new non-PDZ intracellular domain.

The following Nlgn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlgn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01126	NM_134336.2 Latest version!	Rattus norvegicus neuroligin 3 (Nlgn3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa01126	XM_008773251.2 Latest version!	Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa39786	XM_006257054.3 Latest version!	Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa39787	XM_006257055.3 Latest version!	Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlgn3 ( NM_134336.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_599163.2 Rattus norvegicus neuroligin 3 (Nlgn3), mRNA.

Price & Availability↑

CloneID	ORa01126
Clone ID Related Accession (Same CDS sequence)	NM_134336.2 , XM_008773251.2
Accession Version	NM_134336.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	neuroligin-3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U41663.1 and CB771914.1. On Apr 27, 2007 this sequence version replaced NM_134336.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U41663.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCTGC AGCTCGGCCT GCCCTCGTTG TCCCTGAGCC CCACGCCCAC AGTTGGCCGG 
AGCCTGTGCC TCATCCTGTG GTTCCTCAGT TTGGTGCTGA GGGCCAGTAC CCAGGCCCCG 
GCACCCACAG TCAATACTCA CTTTGGGAAA CTAAGGGGTG CCAGAGTACC ATTGCCCAGT 
GAAATCCTGG GTCCTGTGGA CCAATACCTG GGGGTACCCT ACGCAGCTCC CCCGATCGGC 
GAGAAACGTT TCCTGCCCCC TGAACCACCC CCATCCTGGT CGGGCATCCG GAACGCCACA 
CACTTTCCCC CAGTGTGCCC CCAGAACATC CACACAGCTG TGCCCGAAGT CATGCTGCCA 
GTCTGGTTCA CTGCCAACTT GGATATCGTC GCCACTTATA TCCAGGAGCC CAACGAAGAT 
TGCCTCTATC TGAATGTGTA TGTGCCCACG GAAGATGTAA AGCGGATTTC CAAGGAATGC 
GCCCGAAAGC CCAACAAGAA AATTTGTAGG AAAGGAGGAT CCGGCGCTAA GAAACAGGGC 
GAGGACTTAG CGGATAATGA CGGGGATGAA GATGAAGACA TCCGAGACAG TGGTGCTAAA 
CCTGTCATGG TCTACATCCA CGGAGGCTCT TACATGGAAG GAACAGGCAA CATGATTGAC 
GGCAGCGTTC TTGCAAGTTA TGGCAACGTC ATCGTCATCA CACTCAACTA CCGGGTCGGG 
GTGCTAGGTT TCCTGAGCAC TGGGGATCAG GCTGCCAAGG GCAACTATGG GCTCCTTGAT 
CAAATCCAGG CCCTTCGCTG GGTGAGTGAG AACATTGCCT TCTTTGGAGG AGATCCCCGT 
AGAATTACTG TCTTTGGCTC TGGCATTGGT GCATCCTGTG TCAGTCTTCT CACACTGTCT 
CATCACTCTG AGGGGCTTTT CCAGAGGGCC ATCATCCAAA GTGGCTCAGC GCTATCTAGC 
TGGGCTGTGA ACTACCAACC AGTGAAGTAC ACCAGCTTGC TCGCAGACAA AGTGGGCTGT 
AACGTCCTGG ACACTGTGGA TATGGTGGAT TGTCTTCGAC AAAAGAGTGC CAAGGAGCTG 
GTAGAACAGG ACATTCAGCC AGCCCGCTAC CATGTGGCTT TTGGCCCTGT GATTGATGGT 
GATGTCATTC CTGATGACCC TGAGATCCTT ATGGAACAGG GAGAGTTCCT CAACTATGAT 
ATCATGCTAG GCGTCAACCA GGGTGAGGGT CTCAAGTTTG TGGAAGGGGT GGTGGACCCC 
GAGGATGGTG TCTCTGGCAC TGACTTTGAC TACTCTGTCT CCAATTTTGT GGACAATCTA 
TATGGCTATC CCGAGGGTAA GGACACCCTG CGGGAGACTA TCAAGTTCAT GTATACAGAC 
TGGGCAGACC GAGACAACCC TGAGACCCGC CGTAAAACAC TGGTGGCACT CTTCACTGAC 
CACCAGTGGG TGGAGCCTTC GGTGGTGACA GCTGATCTGC ATGCCCGCTA TGGCTCACCC 
ACTTACTTCT ACGCCTTCTA CCATCACTGC CAGAGCCTCA TGAAGCCTGC ATGGTCAGAT 
GCAGCACACG GGGATGAAGT ACCCTATGTT TTTGGTGTCC CTATGGTAGG TCCCACTGAC 
CTTTTCCCCT GCAACTTCTC CAAGAATGAT GTTATGCTCA GTGCTGTTGT TATGACCTAT 
TGGACCAACT TTGCCAAGAC CGGGGATCCC AACAAGCCGG TACCCCAGGA TACCAAGTTC 
ATTCACACCA AGGCCAACCG CTTTGAGGAA GTAGCCTGGT CCAAATACAA TCCCCGGGAC 
CAGCTCTACC TTCACATCGG GCTGAAACCA AGGGTTCGTG ATCATTACCG AGCCACAAAG 
GTAGCCTTTT GGAAGCACCT GGTACCCCAT CTGTACAACC TGCATGACAT GTTCCACTAT 
ACATCCACGA CCACGAAAGT GCCGCCGCCG GACACCACCC ATAGCTCCCA CATCACCCGT 
AGGCCCAACG GCAAGACCTG GAGCACCAAG AGGCCAGCCA TCTCACCTGC CTACAGCAAT 
GAGAATGCTC CTGGGTCCTG GAATGGGGAC CAGGATGCAG GGCCACTCCT GGTCGAGAAC 
CCTCGAGACT ACTCCACTGA ATTAAGTGTC ACCATCGCTG TTGGGGCCTC TCTCCTGTTT 
CTCAATGTGT TGGCCTTTGC TGCTCTCTAT TACCGTAAGG ACAAACGGCG CCAGGAGCCC 
CTGAGGCAGC CTAGCCCCCA AAGGGGAACT GGTGCCCCAG AATTGGGAAC TGCTCCAGAG 
GAGGAGCTGG CAGCATTACA GTTGGGTCCC ACTCACCATG AGTGTGAGGC CGGCCCCCCA 
CACGACACAC TGCGCCTCAC AGCATTGCCC GACTACACCC TGACCCTGCG GCGCTCCCCC 
GATGACATCC CACTCATGAC TCCCAACACC ATCACTATGA TTCCCAATTC CCTGGTTGGG 
TTGCAGACCT TGCACCCCTA TAACACCTTT GCCGCCGGAT TCAACAGCAC CGGGCTGCCC 
CACTCACACT CCACTACCCG TGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599163.2
CDS	302..2848
Translation	MWLQLGLPSLSLSPTPTVGRSLCLILWFLSLVLRASTQAPAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEPPPSWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNEDCLYLNVYVPTEDVKRISKECARKPNKKICRKGGSGAKKQGEDLADNDGDEDEDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSVLASYGNVIVITLNYRVGVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGIGASCVSLLTLSHHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVGCNVLDTVDMVDCLRQKSAKELVEQDIQPARYHVAFGPVIDGDVIPDDPEILMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVVTADLHARYGSPTYFYAFYHHCQSLMKPAWSDAAHGDEVPYVFGVPMVGPTDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKFIHTKANRFEEVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFHYTSTTTKVPPPDTTHSSHITRRPNGKTWSTKRPAISPAYSNENAPGSWNGDQDAGPLLVENPRDYSTELSVTIAVGASLLFLNVLAFAALYYRKDKRRQEPLRQPSPQRGTGAPELGTAPEEELAALQLGPTHHECEAGPPHDTLRLTALPDYTLTLRRSPDDIPLMTPNTITMIPNSLVGLQTLHPYNTFAAGFNSTGLPHSHSTTRV

Target ORF information:

RefSeq Version	NM_134336.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuroligin 3 (Nlgn3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134336.2

ORF Insert Sequence:

ATGTGGCTGC AGCTCGGCCT GCCCTCGTTG TCCCTGAGCC CCACGCCCAC AGTTGGCCGG 
AGCCTGTGCC TCATCCTGTG GTTCCTCAGT TTGGTGCTGA GGGCCAGTAC CCAGGCCCCG 
GCACCCACAG TCAATACTCA CTTTGGGAAA CTAAGGGGTG CCAGAGTACC ATTGCCCAGT 
GAAATCCTGG GTCCTGTGGA CCAATACCTG GGGGTACCCT ACGCAGCTCC CCCGATCGGC 
GAGAAACGTT TCCTGCCCCC TGAACCACCC CCATCCTGGT CGGGCATCCG GAACGCCACA 
CACTTTCCCC CAGTGTGCCC CCAGAACATC CACACAGCTG TGCCCGAAGT CATGCTGCCA 
GTCTGGTTCA CTGCCAACTT GGATATCGTC GCCACTTATA TCCAGGAGCC CAACGAAGAT 
TGCCTCTATC TGAATGTGTA TGTGCCCACG GAAGATGTAA AGCGGATTTC CAAGGAATGC 
GCCCGAAAGC CCAACAAGAA AATTTGTAGG AAAGGAGGAT CCGGCGCTAA GAAACAGGGC 
GAGGACTTAG CGGATAATGA CGGGGATGAA GATGAAGACA TCCGAGACAG TGGTGCTAAA 
CCTGTCATGG TCTACATCCA CGGAGGCTCT TACATGGAAG GAACAGGCAA CATGATTGAC 
GGCAGCGTTC TTGCAAGTTA TGGCAACGTC ATCGTCATCA CACTCAACTA CCGGGTCGGG 
GTGCTAGGTT TCCTGAGCAC TGGGGATCAG GCTGCCAAGG GCAACTATGG GCTCCTTGAT 
CAAATCCAGG CCCTTCGCTG GGTGAGTGAG AACATTGCCT TCTTTGGAGG AGATCCCCGT 
AGAATTACTG TCTTTGGCTC TGGCATTGGT GCATCCTGTG TCAGTCTTCT CACACTGTCT 
CATCACTCTG AGGGGCTTTT CCAGAGGGCC ATCATCCAAA GTGGCTCAGC GCTATCTAGC 
TGGGCTGTGA ACTACCAACC AGTGAAGTAC ACCAGCTTGC TCGCAGACAA AGTGGGCTGT 
AACGTCCTGG ACACTGTGGA TATGGTGGAT TGTCTTCGAC AAAAGAGTGC CAAGGAGCTG 
GTAGAACAGG ACATTCAGCC AGCCCGCTAC CATGTGGCTT TTGGCCCTGT GATTGATGGT 
GATGTCATTC CTGATGACCC TGAGATCCTT ATGGAACAGG GAGAGTTCCT CAACTATGAT 
ATCATGCTAG GCGTCAACCA GGGTGAGGGT CTCAAGTTTG TGGAAGGGGT GGTGGACCCC 
GAGGATGGTG TCTCTGGCAC TGACTTTGAC TACTCTGTCT CCAATTTTGT GGACAATCTA 
TATGGCTATC CCGAGGGTAA GGACACCCTG CGGGAGACTA TCAAGTTCAT GTATACAGAC 
TGGGCAGACC GAGACAACCC TGAGACCCGC CGTAAAACAC TGGTGGCACT CTTCACTGAC 
CACCAGTGGG TGGAGCCTTC GGTGGTGACA GCTGATCTGC ATGCCCGCTA TGGCTCACCC 
ACTTACTTCT ACGCCTTCTA CCATCACTGC CAGAGCCTCA TGAAGCCTGC ATGGTCAGAT 
GCAGCACACG GGGATGAAGT ACCCTATGTT TTTGGTGTCC CTATGGTAGG TCCCACTGAC 
CTTTTCCCCT GCAACTTCTC CAAGAATGAT GTTATGCTCA GTGCTGTTGT TATGACCTAT 
TGGACCAACT TTGCCAAGAC CGGGGATCCC AACAAGCCGG TACCCCAGGA TACCAAGTTC 
ATTCACACCA AGGCCAACCG CTTTGAGGAA GTAGCCTGGT CCAAATACAA TCCCCGGGAC 
CAGCTCTACC TTCACATCGG GCTGAAACCA AGGGTTCGTG ATCATTACCG AGCCACAAAG 
GTAGCCTTTT GGAAGCACCT GGTACCCCAT CTGTACAACC TGCATGACAT GTTCCACTAT 
ACATCCACGA CCACGAAAGT GCCGCCGCCG GACACCACCC ATAGCTCCCA CATCACCCGT 
AGGCCCAACG GCAAGACCTG GAGCACCAAG AGGCCAGCCA TCTCACCTGC CTACAGCAAT 
GAGAATGCTC CTGGGTCCTG GAATGGGGAC CAGGATGCAG GGCCACTCCT GGTCGAGAAC 
CCTCGAGACT ACTCCACTGA ATTAAGTGTC ACCATCGCTG TTGGGGCCTC TCTCCTGTTT 
CTCAATGTGT TGGCCTTTGC TGCTCTCTAT TACCGTAAGG ACAAACGGCG CCAGGAGCCC 
CTGAGGCAGC CTAGCCCCCA AAGGGGAACT GGTGCCCCAG AATTGGGAAC TGCTCCAGAG 
GAGGAGCTGG CAGCATTACA GTTGGGTCCC ACTCACCATG AGTGTGAGGC CGGCCCCCCA 
CACGACACAC TGCGCCTCAC AGCATTGCCC GACTACACCC TGACCCTGCG GCGCTCCCCC 
GATGACATCC CACTCATGAC TCCCAACACC ATCACTATGA TTCCCAATTC CCTGGTTGGG 
TTGCAGACCT TGCACCCCTA TAACACCTTT GCCGCCGGAT TCAACAGCAC CGGGCTGCCC 
CACTCACACT CCACTACCCG TGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlgn3 ( XM_008773251.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771473.1 Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01126
Clone ID Related Accession (Same CDS sequence)	NM_134336.2 , XM_008773251.2
Accession Version	XM_008773251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuroligin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGCTCGGCCT GCCCTCGTTG TCCCTGAGCC CCACGCCCAC AGTTGGCCGG 
AGCCTGTGCC TCATCCTGTG GTTCCTCAGT TTGGTGCTGA GGGCCAGTAC CCAGGCCCCG 
GCACCCACAG TCAATACTCA CTTTGGGAAA CTAAGGGGTG CCAGAGTACC ATTGCCCAGT 
GAAATCCTGG GTCCTGTGGA CCAATACCTG GGGGTACCCT ACGCAGCTCC CCCGATCGGC 
GAGAAACGTT TCCTGCCCCC TGAACCACCC CCATCCTGGT CGGGCATCCG GAACGCCACA 
CACTTTCCCC CAGTGTGCCC CCAGAACATC CACACAGCTG TGCCCGAAGT CATGCTGCCA 
GTCTGGTTCA CTGCCAACTT GGATATCGTC GCCACTTATA TCCAGGAGCC CAACGAAGAT 
TGCCTCTATC TGAATGTGTA TGTGCCCACG GAAGATGTAA AGCGGATTTC CAAGGAATGC 
GCCCGAAAGC CCAACAAGAA AATTTGTAGG AAAGGAGGAT CCGGCGCTAA GAAACAGGGC 
GAGGACTTAG CGGATAATGA CGGGGATGAA GATGAAGACA TCCGAGACAG TGGTGCTAAA 
CCTGTCATGG TCTACATCCA CGGAGGCTCT TACATGGAAG GAACAGGCAA CATGATTGAC 
GGCAGCGTTC TTGCAAGTTA TGGCAACGTC ATCGTCATCA CACTCAACTA CCGGGTCGGG 
GTGCTAGGTT TCCTGAGCAC TGGGGATCAG GCTGCCAAGG GCAACTATGG GCTCCTTGAT 
CAAATCCAGG CCCTTCGCTG GGTGAGTGAG AACATTGCCT TCTTTGGAGG AGATCCCCGT 
AGAATTACTG TCTTTGGCTC TGGCATTGGT GCATCCTGTG TCAGTCTTCT CACACTGTCT 
CATCACTCTG AGGGGCTTTT CCAGAGGGCC ATCATCCAAA GTGGCTCAGC GCTATCTAGC 
TGGGCTGTGA ACTACCAACC AGTGAAGTAC ACCAGCTTGC TCGCAGACAA AGTGGGCTGT 
AACGTCCTGG ACACTGTGGA TATGGTGGAT TGTCTTCGAC AAAAGAGTGC CAAGGAGCTG 
GTAGAACAGG ACATTCAGCC AGCCCGCTAC CATGTGGCTT TTGGCCCTGT GATTGATGGT 
GATGTCATTC CTGATGACCC TGAGATCCTT ATGGAACAGG GAGAGTTCCT CAACTATGAT 
ATCATGCTAG GCGTCAACCA GGGTGAGGGT CTCAAGTTTG TGGAAGGGGT GGTGGACCCC 
GAGGATGGTG TCTCTGGCAC TGACTTTGAC TACTCTGTCT CCAATTTTGT GGACAATCTA 
TATGGCTATC CCGAGGGTAA GGACACCCTG CGGGAGACTA TCAAGTTCAT GTATACAGAC 
TGGGCAGACC GAGACAACCC TGAGACCCGC CGTAAAACAC TGGTGGCACT CTTCACTGAC 
CACCAGTGGG TGGAGCCTTC GGTGGTGACA GCTGATCTGC ATGCCCGCTA TGGCTCACCC 
ACTTACTTCT ACGCCTTCTA CCATCACTGC CAGAGCCTCA TGAAGCCTGC ATGGTCAGAT 
GCAGCACACG GGGATGAAGT ACCCTATGTT TTTGGTGTCC CTATGGTAGG TCCCACTGAC 
CTTTTCCCCT GCAACTTCTC CAAGAATGAT GTTATGCTCA GTGCTGTTGT TATGACCTAT 
TGGACCAACT TTGCCAAGAC CGGGGATCCC AACAAGCCGG TACCCCAGGA TACCAAGTTC 
ATTCACACCA AGGCCAACCG CTTTGAGGAA GTAGCCTGGT CCAAATACAA TCCCCGGGAC 
CAGCTCTACC TTCACATCGG GCTGAAACCA AGGGTTCGTG ATCATTACCG AGCCACAAAG 
GTAGCCTTTT GGAAGCACCT GGTACCCCAT CTGTACAACC TGCATGACAT GTTCCACTAT 
ACATCCACGA CCACGAAAGT GCCGCCGCCG GACACCACCC ATAGCTCCCA CATCACCCGT 
AGGCCCAACG GCAAGACCTG GAGCACCAAG AGGCCAGCCA TCTCACCTGC CTACAGCAAT 
GAGAATGCTC CTGGGTCCTG GAATGGGGAC CAGGATGCAG GGCCACTCCT GGTCGAGAAC 
CCTCGAGACT ACTCCACTGA ATTAAGTGTC ACCATCGCTG TTGGGGCCTC TCTCCTGTTT 
CTCAATGTGT TGGCCTTTGC TGCTCTCTAT TACCGTAAGG ACAAACGGCG CCAGGAGCCC 
CTGAGGCAGC CTAGCCCCCA AAGGGGAACT GGTGCCCCAG AATTGGGAAC TGCTCCAGAG 
GAGGAGCTGG CAGCATTACA GTTGGGTCCC ACTCACCATG AGTGTGAGGC CGGCCCCCCA 
CACGACACAC TGCGCCTCAC AGCATTGCCC GACTACACCC TGACCCTGCG GCGCTCCCCC 
GATGACATCC CACTCATGAC TCCCAACACC ATCACTATGA TTCCCAATTC CCTGGTTGGG 
TTGCAGACCT TGCACCCCTA TAACACCTTT GCCGCCGGAT TCAACAGCAC CGGGCTGCCC 
CACTCACACT CCACTACCCG TGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771473.1
CDS	428..2974
Translation	MWLQLGLPSLSLSPTPTVGRSLCLILWFLSLVLRASTQAPAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEPPPSWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNEDCLYLNVYVPTEDVKRISKECARKPNKKICRKGGSGAKKQGEDLADNDGDEDEDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSVLASYGNVIVITLNYRVGVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGIGASCVSLLTLSHHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVGCNVLDTVDMVDCLRQKSAKELVEQDIQPARYHVAFGPVIDGDVIPDDPEILMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVVTADLHARYGSPTYFYAFYHHCQSLMKPAWSDAAHGDEVPYVFGVPMVGPTDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKFIHTKANRFEEVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFHYTSTTTKVPPPDTTHSSHITRRPNGKTWSTKRPAISPAYSNENAPGSWNGDQDAGPLLVENPRDYSTELSVTIAVGASLLFLNVLAFAALYYRKDKRRQEPLRQPSPQRGTGAPELGTAPEEELAALQLGPTHHECEAGPPHDTLRLTALPDYTLTLRRSPDDIPLMTPNTITMIPNSLVGLQTLHPYNTFAAGFNSTGLPHSHSTTRV

Target ORF information:

RefSeq Version	XM_008773251.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773251.2

ORF Insert Sequence:

ATGTGGCTGC AGCTCGGCCT GCCCTCGTTG TCCCTGAGCC CCACGCCCAC AGTTGGCCGG 
AGCCTGTGCC TCATCCTGTG GTTCCTCAGT TTGGTGCTGA GGGCCAGTAC CCAGGCCCCG 
GCACCCACAG TCAATACTCA CTTTGGGAAA CTAAGGGGTG CCAGAGTACC ATTGCCCAGT 
GAAATCCTGG GTCCTGTGGA CCAATACCTG GGGGTACCCT ACGCAGCTCC CCCGATCGGC 
GAGAAACGTT TCCTGCCCCC TGAACCACCC CCATCCTGGT CGGGCATCCG GAACGCCACA 
CACTTTCCCC CAGTGTGCCC CCAGAACATC CACACAGCTG TGCCCGAAGT CATGCTGCCA 
GTCTGGTTCA CTGCCAACTT GGATATCGTC GCCACTTATA TCCAGGAGCC CAACGAAGAT 
TGCCTCTATC TGAATGTGTA TGTGCCCACG GAAGATGTAA AGCGGATTTC CAAGGAATGC 
GCCCGAAAGC CCAACAAGAA AATTTGTAGG AAAGGAGGAT CCGGCGCTAA GAAACAGGGC 
GAGGACTTAG CGGATAATGA CGGGGATGAA GATGAAGACA TCCGAGACAG TGGTGCTAAA 
CCTGTCATGG TCTACATCCA CGGAGGCTCT TACATGGAAG GAACAGGCAA CATGATTGAC 
GGCAGCGTTC TTGCAAGTTA TGGCAACGTC ATCGTCATCA CACTCAACTA CCGGGTCGGG 
GTGCTAGGTT TCCTGAGCAC TGGGGATCAG GCTGCCAAGG GCAACTATGG GCTCCTTGAT 
CAAATCCAGG CCCTTCGCTG GGTGAGTGAG AACATTGCCT TCTTTGGAGG AGATCCCCGT 
AGAATTACTG TCTTTGGCTC TGGCATTGGT GCATCCTGTG TCAGTCTTCT CACACTGTCT 
CATCACTCTG AGGGGCTTTT CCAGAGGGCC ATCATCCAAA GTGGCTCAGC GCTATCTAGC 
TGGGCTGTGA ACTACCAACC AGTGAAGTAC ACCAGCTTGC TCGCAGACAA AGTGGGCTGT 
AACGTCCTGG ACACTGTGGA TATGGTGGAT TGTCTTCGAC AAAAGAGTGC CAAGGAGCTG 
GTAGAACAGG ACATTCAGCC AGCCCGCTAC CATGTGGCTT TTGGCCCTGT GATTGATGGT 
GATGTCATTC CTGATGACCC TGAGATCCTT ATGGAACAGG GAGAGTTCCT CAACTATGAT 
ATCATGCTAG GCGTCAACCA GGGTGAGGGT CTCAAGTTTG TGGAAGGGGT GGTGGACCCC 
GAGGATGGTG TCTCTGGCAC TGACTTTGAC TACTCTGTCT CCAATTTTGT GGACAATCTA 
TATGGCTATC CCGAGGGTAA GGACACCCTG CGGGAGACTA TCAAGTTCAT GTATACAGAC 
TGGGCAGACC GAGACAACCC TGAGACCCGC CGTAAAACAC TGGTGGCACT CTTCACTGAC 
CACCAGTGGG TGGAGCCTTC GGTGGTGACA GCTGATCTGC ATGCCCGCTA TGGCTCACCC 
ACTTACTTCT ACGCCTTCTA CCATCACTGC CAGAGCCTCA TGAAGCCTGC ATGGTCAGAT 
GCAGCACACG GGGATGAAGT ACCCTATGTT TTTGGTGTCC CTATGGTAGG TCCCACTGAC 
CTTTTCCCCT GCAACTTCTC CAAGAATGAT GTTATGCTCA GTGCTGTTGT TATGACCTAT 
TGGACCAACT TTGCCAAGAC CGGGGATCCC AACAAGCCGG TACCCCAGGA TACCAAGTTC 
ATTCACACCA AGGCCAACCG CTTTGAGGAA GTAGCCTGGT CCAAATACAA TCCCCGGGAC 
CAGCTCTACC TTCACATCGG GCTGAAACCA AGGGTTCGTG ATCATTACCG AGCCACAAAG 
GTAGCCTTTT GGAAGCACCT GGTACCCCAT CTGTACAACC TGCATGACAT GTTCCACTAT 
ACATCCACGA CCACGAAAGT GCCGCCGCCG GACACCACCC ATAGCTCCCA CATCACCCGT 
AGGCCCAACG GCAAGACCTG GAGCACCAAG AGGCCAGCCA TCTCACCTGC CTACAGCAAT 
GAGAATGCTC CTGGGTCCTG GAATGGGGAC CAGGATGCAG GGCCACTCCT GGTCGAGAAC 
CCTCGAGACT ACTCCACTGA ATTAAGTGTC ACCATCGCTG TTGGGGCCTC TCTCCTGTTT 
CTCAATGTGT TGGCCTTTGC TGCTCTCTAT TACCGTAAGG ACAAACGGCG CCAGGAGCCC 
CTGAGGCAGC CTAGCCCCCA AAGGGGAACT GGTGCCCCAG AATTGGGAAC TGCTCCAGAG 
GAGGAGCTGG CAGCATTACA GTTGGGTCCC ACTCACCATG AGTGTGAGGC CGGCCCCCCA 
CACGACACAC TGCGCCTCAC AGCATTGCCC GACTACACCC TGACCCTGCG GCGCTCCCCC 
GATGACATCC CACTCATGAC TCCCAACACC ATCACTATGA TTCCCAATTC CCTGGTTGGG 
TTGCAGACCT TGCACCCCTA TAACACCTTT GCCGCCGGAT TCAACAGCAC CGGGCTGCCC 
CACTCACACT CCACTACCCG TGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlgn3 ( XM_006257054.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257116.1 Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa39786
Clone ID Related Accession (Same CDS sequence)	XM_006257054.3
Accession Version	XM_006257054.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuroligin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGCTCGGCCT GCCCTCGTTG TCCCTGAGCC CCACGCCCAC AGTTGGCCGG 
AGCCTGTGCC TCATCCTGTG GTTCCTCAGT TTGGTGCTGA GGGCCAGTAC CCAGGCCCCG 
GCACCCACAG TCAATACTCA CTTTGGGAAA CTAAGGGGTG CCAGAGTACC ATTGCCCAGT 
GAAATCCTGG GTCCTGTGGA CCAATACCTG GGGGTACCCT ACGCAGCTCC CCCGATCGGC 
GAGAAACGTT TCCTGCCCCC TGAACCACCC CCATCCTGGT CGGGCATCCG GAACGCCACA 
CACTTTCCCC CAGTGTGCCC CCAGAACATC CACACAGCTG TGCCCGAAGT CATGCTGCCA 
GTCTGGTTCA CTGCCAACTT GGATATCGTC GCCACTTATA TCCAGGAGCC CAACGAAGAT 
TGCCTCTATC TGAATGTGTA TGTGCCCACG GAAGATGGAT CCGGCGCTAA GAAACAGGGC 
GAGGACTTAG CGGATAATGA CGGGGATGAA GATGAAGACA TCCGAGACAG TGGTGCTAAA 
CCTGTCATGG TCTACATCCA CGGAGGCTCT TACATGGAAG GAACAGGCAA CATGATTGAC 
GGCAGCGTTC TTGCAAGTTA TGGCAACGTC ATCGTCATCA CACTCAACTA CCGGGTCGGG 
GTGCTAGGTT TCCTGAGCAC TGGGGATCAG GCTGCCAAGG GCAACTATGG GCTCCTTGAT 
CAAATCCAGG CCCTTCGCTG GGTGAGTGAG AACATTGCCT TCTTTGGAGG AGATCCCCGT 
AGAATTACTG TCTTTGGCTC TGGCATTGGT GCATCCTGTG TCAGTCTTCT CACACTGTCT 
CATCACTCTG AGGGGCTTTT CCAGAGGGCC ATCATCCAAA GTGGCTCAGC GCTATCTAGC 
TGGGCTGTGA ACTACCAACC AGTGAAGTAC ACCAGCTTGC TCGCAGACAA AGTGGGCTGT 
AACGTCCTGG ACACTGTGGA TATGGTGGAT TGTCTTCGAC AAAAGAGTGC CAAGGAGCTG 
GTAGAACAGG ACATTCAGCC AGCCCGCTAC CATGTGGCTT TTGGCCCTGT GATTGATGGT 
GATGTCATTC CTGATGACCC TGAGATCCTT ATGGAACAGG GAGAGTTCCT CAACTATGAT 
ATCATGCTAG GCGTCAACCA GGGTGAGGGT CTCAAGTTTG TGGAAGGGGT GGTGGACCCC 
GAGGATGGTG TCTCTGGCAC TGACTTTGAC TACTCTGTCT CCAATTTTGT GGACAATCTA 
TATGGCTATC CCGAGGGTAA GGACACCCTG CGGGAGACTA TCAAGTTCAT GTATACAGAC 
TGGGCAGACC GAGACAACCC TGAGACCCGC CGTAAAACAC TGGTGGCACT CTTCACTGAC 
CACCAGTGGG TGGAGCCTTC GGTGGTGACA GCTGATCTGC ATGCCCGCTA TGGCTCACCC 
ACTTACTTCT ACGCCTTCTA CCATCACTGC CAGAGCCTCA TGAAGCCTGC ATGGTCAGAT 
GCAGCACACG GGGATGAAGT ACCCTATGTT TTTGGTGTCC CTATGGTAGG TCCCACTGAC 
CTTTTCCCCT GCAACTTCTC CAAGAATGAT GTTATGCTCA GTGCTGTTGT TATGACCTAT 
TGGACCAACT TTGCCAAGAC CGGGGATCCC AACAAGCCGG TACCCCAGGA TACCAAGTTC 
ATTCACACCA AGGCCAACCG CTTTGAGGAA GTAGCCTGGT CCAAATACAA TCCCCGGGAC 
CAGCTCTACC TTCACATCGG GCTGAAACCA AGGGTTCGTG ATCATTACCG AGCCACAAAG 
GTAGCCTTTT GGAAGCACCT GGTACCCCAT CTGTACAACC TGCATGACAT GTTCCACTAT 
ACATCCACGA CCACGAAAGT GCCGCCGCCG GACACCACCC ATAGCTCCCA CATCACCCGT 
AGGCCCAACG GCAAGACCTG GAGCACCAAG AGGCCAGCCA TCTCACCTGC CTACAGCAAT 
GAGAATGCTC CTGGGTCCTG GAATGGGGAC CAGGATGCAG GGCCACTCCT GGTCGAGAAC 
CCTCGAGACT ACTCCACTGA ATTAAGTGTC ACCATCGCTG TTGGGGCCTC TCTCCTGTTT 
CTCAATGTGT TGGCCTTTGC TGCTCTCTAT TACCGTAAGG ACAAACGGCG CCAGGAGCCC 
CTGAGGCAGC CTAGCCCCCA AAGGGGAACT GGTGCCCCAG AATTGGGAAC TGCTCCAGAG 
GAGGAGCTGG CAGCATTACA GTTGGGTCCC ACTCACCATG AGTGTGAGGC CGGCCCCCCA 
CACGACACAC TGCGCCTCAC AGCATTGCCC GACTACACCC TGACCCTGCG GCGCTCCCCC 
GATGACATCC CACTCATGAC TCCCAACACC ATCACTATGA TTCCCAATTC CCTGGTTGGG 
TTGCAGACCT TGCACCCCTA TAACACCTTT GCCGCCGGAT TCAACAGCAC CGGGCTGCCC 
CACTCACACT CCACTACCCG TGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257116.1
CDS	428..2914
Translation	MWLQLGLPSLSLSPTPTVGRSLCLILWFLSLVLRASTQAPAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEPPPSWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNEDCLYLNVYVPTEDGSGAKKQGEDLADNDGDEDEDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSVLASYGNVIVITLNYRVGVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGIGASCVSLLTLSHHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVGCNVLDTVDMVDCLRQKSAKELVEQDIQPARYHVAFGPVIDGDVIPDDPEILMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVVTADLHARYGSPTYFYAFYHHCQSLMKPAWSDAAHGDEVPYVFGVPMVGPTDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKFIHTKANRFEEVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFHYTSTTTKVPPPDTTHSSHITRRPNGKTWSTKRPAISPAYSNENAPGSWNGDQDAGPLLVENPRDYSTELSVTIAVGASLLFLNVLAFAALYYRKDKRRQEPLRQPSPQRGTGAPELGTAPEEELAALQLGPTHHECEAGPPHDTLRLTALPDYTLTLRRSPDDIPLMTPNTITMIPNSLVGLQTLHPYNTFAAGFNSTGLPHSHSTTRV

Target ORF information:

RefSeq Version	XM_006257054.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257054.3

ORF Insert Sequence:

ATGTGGCTGC AGCTCGGCCT GCCCTCGTTG TCCCTGAGCC CCACGCCCAC AGTTGGCCGG 
AGCCTGTGCC TCATCCTGTG GTTCCTCAGT TTGGTGCTGA GGGCCAGTAC CCAGGCCCCG 
GCACCCACAG TCAATACTCA CTTTGGGAAA CTAAGGGGTG CCAGAGTACC ATTGCCCAGT 
GAAATCCTGG GTCCTGTGGA CCAATACCTG GGGGTACCCT ACGCAGCTCC CCCGATCGGC 
GAGAAACGTT TCCTGCCCCC TGAACCACCC CCATCCTGGT CGGGCATCCG GAACGCCACA 
CACTTTCCCC CAGTGTGCCC CCAGAACATC CACACAGCTG TGCCCGAAGT CATGCTGCCA 
GTCTGGTTCA CTGCCAACTT GGATATCGTC GCCACTTATA TCCAGGAGCC CAACGAAGAT 
TGCCTCTATC TGAATGTGTA TGTGCCCACG GAAGATGGAT CCGGCGCTAA GAAACAGGGC 
GAGGACTTAG CGGATAATGA CGGGGATGAA GATGAAGACA TCCGAGACAG TGGTGCTAAA 
CCTGTCATGG TCTACATCCA CGGAGGCTCT TACATGGAAG GAACAGGCAA CATGATTGAC 
GGCAGCGTTC TTGCAAGTTA TGGCAACGTC ATCGTCATCA CACTCAACTA CCGGGTCGGG 
GTGCTAGGTT TCCTGAGCAC TGGGGATCAG GCTGCCAAGG GCAACTATGG GCTCCTTGAT 
CAAATCCAGG CCCTTCGCTG GGTGAGTGAG AACATTGCCT TCTTTGGAGG AGATCCCCGT 
AGAATTACTG TCTTTGGCTC TGGCATTGGT GCATCCTGTG TCAGTCTTCT CACACTGTCT 
CATCACTCTG AGGGGCTTTT CCAGAGGGCC ATCATCCAAA GTGGCTCAGC GCTATCTAGC 
TGGGCTGTGA ACTACCAACC AGTGAAGTAC ACCAGCTTGC TCGCAGACAA AGTGGGCTGT 
AACGTCCTGG ACACTGTGGA TATGGTGGAT TGTCTTCGAC AAAAGAGTGC CAAGGAGCTG 
GTAGAACAGG ACATTCAGCC AGCCCGCTAC CATGTGGCTT TTGGCCCTGT GATTGATGGT 
GATGTCATTC CTGATGACCC TGAGATCCTT ATGGAACAGG GAGAGTTCCT CAACTATGAT 
ATCATGCTAG GCGTCAACCA GGGTGAGGGT CTCAAGTTTG TGGAAGGGGT GGTGGACCCC 
GAGGATGGTG TCTCTGGCAC TGACTTTGAC TACTCTGTCT CCAATTTTGT GGACAATCTA 
TATGGCTATC CCGAGGGTAA GGACACCCTG CGGGAGACTA TCAAGTTCAT GTATACAGAC 
TGGGCAGACC GAGACAACCC TGAGACCCGC CGTAAAACAC TGGTGGCACT CTTCACTGAC 
CACCAGTGGG TGGAGCCTTC GGTGGTGACA GCTGATCTGC ATGCCCGCTA TGGCTCACCC 
ACTTACTTCT ACGCCTTCTA CCATCACTGC CAGAGCCTCA TGAAGCCTGC ATGGTCAGAT 
GCAGCACACG GGGATGAAGT ACCCTATGTT TTTGGTGTCC CTATGGTAGG TCCCACTGAC 
CTTTTCCCCT GCAACTTCTC CAAGAATGAT GTTATGCTCA GTGCTGTTGT TATGACCTAT 
TGGACCAACT TTGCCAAGAC CGGGGATCCC AACAAGCCGG TACCCCAGGA TACCAAGTTC 
ATTCACACCA AGGCCAACCG CTTTGAGGAA GTAGCCTGGT CCAAATACAA TCCCCGGGAC 
CAGCTCTACC TTCACATCGG GCTGAAACCA AGGGTTCGTG ATCATTACCG AGCCACAAAG 
GTAGCCTTTT GGAAGCACCT GGTACCCCAT CTGTACAACC TGCATGACAT GTTCCACTAT 
ACATCCACGA CCACGAAAGT GCCGCCGCCG GACACCACCC ATAGCTCCCA CATCACCCGT 
AGGCCCAACG GCAAGACCTG GAGCACCAAG AGGCCAGCCA TCTCACCTGC CTACAGCAAT 
GAGAATGCTC CTGGGTCCTG GAATGGGGAC CAGGATGCAG GGCCACTCCT GGTCGAGAAC 
CCTCGAGACT ACTCCACTGA ATTAAGTGTC ACCATCGCTG TTGGGGCCTC TCTCCTGTTT 
CTCAATGTGT TGGCCTTTGC TGCTCTCTAT TACCGTAAGG ACAAACGGCG CCAGGAGCCC 
CTGAGGCAGC CTAGCCCCCA AAGGGGAACT GGTGCCCCAG AATTGGGAAC TGCTCCAGAG 
GAGGAGCTGG CAGCATTACA GTTGGGTCCC ACTCACCATG AGTGTGAGGC CGGCCCCCCA 
CACGACACAC TGCGCCTCAC AGCATTGCCC GACTACACCC TGACCCTGCG GCGCTCCCCC 
GATGACATCC CACTCATGAC TCCCAACACC ATCACTATGA TTCCCAATTC CCTGGTTGGG 
TTGCAGACCT TGCACCCCTA TAACACCTTT GCCGCCGGAT TCAACAGCAC CGGGCTGCCC 
CACTCACACT CCACTACCCG TGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlgn3 ( XM_006257055.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257117.1 Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa39787
Clone ID Related Accession (Same CDS sequence)	XM_006257055.3
Accession Version	XM_006257055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuroligin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGCTCGGCCT GCCCTCGTTG TCCCTGAGCC CCACGCCCAC AGTTGGCCGG 
AGCCTGTGCC TCATCCTGTG GTTCCTCAGT TTGGTGCTGA GGGCCAGTAC CCAGGCCCCG 
GCACCCACAG TCAATACTCA CTTTGGGAAA CTAAGGGGTG CCAGAGTACC ATTGCCCAGT 
GAAATCCTGG GTCCTGTGGA CCAATACCTG GGGGTACCCT ACGCAGCTCC CCCGATCGGC 
GAGAAACGTT TCCTGCCCCC TGAACCACCC CCATCCTGGT CGGGCATCCG GAACGCCACA 
CACTTTCCCC CAGTGTGCCC CCAGAACATC CACACAGCTG TGCCCGAAGT CATGCTGCCA 
GTCTGGTTCA CTGCCAACTT GGATATCGTC GCCACTTATA TCCAGGAGCC CAACGAAGAT 
TGCCTCTATC TGAATGTGTA TGTGCCCACG GAAGATGACA TCCGAGACAG TGGTGCTAAA 
CCTGTCATGG TCTACATCCA CGGAGGCTCT TACATGGAAG GAACAGGCAA CATGATTGAC 
GGCAGCGTTC TTGCAAGTTA TGGCAACGTC ATCGTCATCA CACTCAACTA CCGGGTCGGG 
GTGCTAGGTT TCCTGAGCAC TGGGGATCAG GCTGCCAAGG GCAACTATGG GCTCCTTGAT 
CAAATCCAGG CCCTTCGCTG GGTGAGTGAG AACATTGCCT TCTTTGGAGG AGATCCCCGT 
AGAATTACTG TCTTTGGCTC TGGCATTGGT GCATCCTGTG TCAGTCTTCT CACACTGTCT 
CATCACTCTG AGGGGCTTTT CCAGAGGGCC ATCATCCAAA GTGGCTCAGC GCTATCTAGC 
TGGGCTGTGA ACTACCAACC AGTGAAGTAC ACCAGCTTGC TCGCAGACAA AGTGGGCTGT 
AACGTCCTGG ACACTGTGGA TATGGTGGAT TGTCTTCGAC AAAAGAGTGC CAAGGAGCTG 
GTAGAACAGG ACATTCAGCC AGCCCGCTAC CATGTGGCTT TTGGCCCTGT GATTGATGGT 
GATGTCATTC CTGATGACCC TGAGATCCTT ATGGAACAGG GAGAGTTCCT CAACTATGAT 
ATCATGCTAG GCGTCAACCA GGGTGAGGGT CTCAAGTTTG TGGAAGGGGT GGTGGACCCC 
GAGGATGGTG TCTCTGGCAC TGACTTTGAC TACTCTGTCT CCAATTTTGT GGACAATCTA 
TATGGCTATC CCGAGGGTAA GGACACCCTG CGGGAGACTA TCAAGTTCAT GTATACAGAC 
TGGGCAGACC GAGACAACCC TGAGACCCGC CGTAAAACAC TGGTGGCACT CTTCACTGAC 
CACCAGTGGG TGGAGCCTTC GGTGGTGACA GCTGATCTGC ATGCCCGCTA TGGCTCACCC 
ACTTACTTCT ACGCCTTCTA CCATCACTGC CAGAGCCTCA TGAAGCCTGC ATGGTCAGAT 
GCAGCACACG GGGATGAAGT ACCCTATGTT TTTGGTGTCC CTATGGTAGG TCCCACTGAC 
CTTTTCCCCT GCAACTTCTC CAAGAATGAT GTTATGCTCA GTGCTGTTGT TATGACCTAT 
TGGACCAACT TTGCCAAGAC CGGGGATCCC AACAAGCCGG TACCCCAGGA TACCAAGTTC 
ATTCACACCA AGGCCAACCG CTTTGAGGAA GTAGCCTGGT CCAAATACAA TCCCCGGGAC 
CAGCTCTACC TTCACATCGG GCTGAAACCA AGGGTTCGTG ATCATTACCG AGCCACAAAG 
GTAGCCTTTT GGAAGCACCT GGTACCCCAT CTGTACAACC TGCATGACAT GTTCCACTAT 
ACATCCACGA CCACGAAAGT GCCGCCGCCG GACACCACCC ATAGCTCCCA CATCACCCGT 
AGGCCCAACG GCAAGACCTG GAGCACCAAG AGGCCAGCCA TCTCACCTGC CTACAGCAAT 
GAGAATGCTC CTGGGTCCTG GAATGGGGAC CAGGATGCAG GGCCACTCCT GGTCGAGAAC 
CCTCGAGACT ACTCCACTGA ATTAAGTGTC ACCATCGCTG TTGGGGCCTC TCTCCTGTTT 
CTCAATGTGT TGGCCTTTGC TGCTCTCTAT TACCGTAAGG ACAAACGGCG CCAGGAGCCC 
CTGAGGCAGC CTAGCCCCCA AAGGGGAACT GGTGCCCCAG AATTGGGAAC TGCTCCAGAG 
GAGGAGCTGG CAGCATTACA GTTGGGTCCC ACTCACCATG AGTGTGAGGC CGGCCCCCCA 
CACGACACAC TGCGCCTCAC AGCATTGCCC GACTACACCC TGACCCTGCG GCGCTCCCCC 
GATGACATCC CACTCATGAC TCCCAACACC ATCACTATGA TTCCCAATTC CCTGGTTGGG 
TTGCAGACCT TGCACCCCTA TAACACCTTT GCCGCCGGAT TCAACAGCAC CGGGCTGCCC 
CACTCACACT CCACTACCCG TGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257117.1
CDS	428..2854
Translation	MWLQLGLPSLSLSPTPTVGRSLCLILWFLSLVLRASTQAPAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEPPPSWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNEDCLYLNVYVPTEDDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSVLASYGNVIVITLNYRVGVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGIGASCVSLLTLSHHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVGCNVLDTVDMVDCLRQKSAKELVEQDIQPARYHVAFGPVIDGDVIPDDPEILMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVVTADLHARYGSPTYFYAFYHHCQSLMKPAWSDAAHGDEVPYVFGVPMVGPTDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKFIHTKANRFEEVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFHYTSTTTKVPPPDTTHSSHITRRPNGKTWSTKRPAISPAYSNENAPGSWNGDQDAGPLLVENPRDYSTELSVTIAVGASLLFLNVLAFAALYYRKDKRRQEPLRQPSPQRGTGAPELGTAPEEELAALQLGPTHHECEAGPPHDTLRLTALPDYTLTLRRSPDDIPLMTPNTITMIPNSLVGLQTLHPYNTFAAGFNSTGLPHSHSTTRV

Target ORF information:

RefSeq Version	XM_006257055.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuroligin 3 (Nlgn3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257055.3

ORF Insert Sequence:

ATGTGGCTGC AGCTCGGCCT GCCCTCGTTG TCCCTGAGCC CCACGCCCAC AGTTGGCCGG 
AGCCTGTGCC TCATCCTGTG GTTCCTCAGT TTGGTGCTGA GGGCCAGTAC CCAGGCCCCG 
GCACCCACAG TCAATACTCA CTTTGGGAAA CTAAGGGGTG CCAGAGTACC ATTGCCCAGT 
GAAATCCTGG GTCCTGTGGA CCAATACCTG GGGGTACCCT ACGCAGCTCC CCCGATCGGC 
GAGAAACGTT TCCTGCCCCC TGAACCACCC CCATCCTGGT CGGGCATCCG GAACGCCACA 
CACTTTCCCC CAGTGTGCCC CCAGAACATC CACACAGCTG TGCCCGAAGT CATGCTGCCA 
GTCTGGTTCA CTGCCAACTT GGATATCGTC GCCACTTATA TCCAGGAGCC CAACGAAGAT 
TGCCTCTATC TGAATGTGTA TGTGCCCACG GAAGATGACA TCCGAGACAG TGGTGCTAAA 
CCTGTCATGG TCTACATCCA CGGAGGCTCT TACATGGAAG GAACAGGCAA CATGATTGAC 
GGCAGCGTTC TTGCAAGTTA TGGCAACGTC ATCGTCATCA CACTCAACTA CCGGGTCGGG 
GTGCTAGGTT TCCTGAGCAC TGGGGATCAG GCTGCCAAGG GCAACTATGG GCTCCTTGAT 
CAAATCCAGG CCCTTCGCTG GGTGAGTGAG AACATTGCCT TCTTTGGAGG AGATCCCCGT 
AGAATTACTG TCTTTGGCTC TGGCATTGGT GCATCCTGTG TCAGTCTTCT CACACTGTCT 
CATCACTCTG AGGGGCTTTT CCAGAGGGCC ATCATCCAAA GTGGCTCAGC GCTATCTAGC 
TGGGCTGTGA ACTACCAACC AGTGAAGTAC ACCAGCTTGC TCGCAGACAA AGTGGGCTGT 
AACGTCCTGG ACACTGTGGA TATGGTGGAT TGTCTTCGAC AAAAGAGTGC CAAGGAGCTG 
GTAGAACAGG ACATTCAGCC AGCCCGCTAC CATGTGGCTT TTGGCCCTGT GATTGATGGT 
GATGTCATTC CTGATGACCC TGAGATCCTT ATGGAACAGG GAGAGTTCCT CAACTATGAT 
ATCATGCTAG GCGTCAACCA GGGTGAGGGT CTCAAGTTTG TGGAAGGGGT GGTGGACCCC 
GAGGATGGTG TCTCTGGCAC TGACTTTGAC TACTCTGTCT CCAATTTTGT GGACAATCTA 
TATGGCTATC CCGAGGGTAA GGACACCCTG CGGGAGACTA TCAAGTTCAT GTATACAGAC 
TGGGCAGACC GAGACAACCC TGAGACCCGC CGTAAAACAC TGGTGGCACT CTTCACTGAC 
CACCAGTGGG TGGAGCCTTC GGTGGTGACA GCTGATCTGC ATGCCCGCTA TGGCTCACCC 
ACTTACTTCT ACGCCTTCTA CCATCACTGC CAGAGCCTCA TGAAGCCTGC ATGGTCAGAT 
GCAGCACACG GGGATGAAGT ACCCTATGTT TTTGGTGTCC CTATGGTAGG TCCCACTGAC 
CTTTTCCCCT GCAACTTCTC CAAGAATGAT GTTATGCTCA GTGCTGTTGT TATGACCTAT 
TGGACCAACT TTGCCAAGAC CGGGGATCCC AACAAGCCGG TACCCCAGGA TACCAAGTTC 
ATTCACACCA AGGCCAACCG CTTTGAGGAA GTAGCCTGGT CCAAATACAA TCCCCGGGAC 
CAGCTCTACC TTCACATCGG GCTGAAACCA AGGGTTCGTG ATCATTACCG AGCCACAAAG 
GTAGCCTTTT GGAAGCACCT GGTACCCCAT CTGTACAACC TGCATGACAT GTTCCACTAT 
ACATCCACGA CCACGAAAGT GCCGCCGCCG GACACCACCC ATAGCTCCCA CATCACCCGT 
AGGCCCAACG GCAAGACCTG GAGCACCAAG AGGCCAGCCA TCTCACCTGC CTACAGCAAT 
GAGAATGCTC CTGGGTCCTG GAATGGGGAC CAGGATGCAG GGCCACTCCT GGTCGAGAAC 
CCTCGAGACT ACTCCACTGA ATTAAGTGTC ACCATCGCTG TTGGGGCCTC TCTCCTGTTT 
CTCAATGTGT TGGCCTTTGC TGCTCTCTAT TACCGTAAGG ACAAACGGCG CCAGGAGCCC 
CTGAGGCAGC CTAGCCCCCA AAGGGGAACT GGTGCCCCAG AATTGGGAAC TGCTCCAGAG 
GAGGAGCTGG CAGCATTACA GTTGGGTCCC ACTCACCATG AGTGTGAGGC CGGCCCCCCA 
CACGACACAC TGCGCCTCAC AGCATTGCCC GACTACACCC TGACCCTGCG GCGCTCCCCC 
GATGACATCC CACTCATGAC TCCCAACACC ATCACTATGA TTCCCAATTC CCTGGTTGGG 
TTGCAGACCT TGCACCCCTA TAACACCTTT GCCGCCGGAT TCAACAGCAC CGGGCTGCCC 
CACTCACACT CCACTACCCG TGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct roles for extracellular and intracellular domains in neuroligin function at inhibitory synapses.
eLife5(2016 11)
Nguyen QA,Horn ME,Nicoll RA

Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system.
The Biochemical journal473(4)423-34(2016 Feb)
Ulbrich L,Favaloro FL,Trobiani L,Marchetti V,Patel V,Pascucci T,Comoletti D,Marciniak SJ,De Jaco A

In vivo clonal overexpression of neuroligin 3 and neuroligin 2 in neurons of the rat cerebral cortex: Differential effects on GABAergic synapses and neuronal migration.
The Journal of comparative neurology523(9)1359-78(2015 Jun)
Fekete CD,Chiou TT,Miralles CP,Harris RS,Fiondella CG,Loturco JJ,De Blas AL

Fmr1 and Nlgn3 knockout rats: novel tools for investigating autism spectrum disorders.
Behavioral neuroscience128(2)103-9(2014 Apr)
Hamilton SM,Green JR,Veeraragavan S,Yuva L,McCoy A,Wu Y,Warren J,Little L,Ji D,Cui X,Weinstein E,Paylor R

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Nlgn3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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