Nlg4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Nlg4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlg4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa05458 NM_001170191.2
Latest version!
Drosophila melanogaster neuroligin 4, transcript variant D (Nlg4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa05459 NM_001300491.1
Latest version!
Drosophila melanogaster neuroligin 4, transcript variant E (Nlg4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa05459 NM_001170190.2
Latest version!
Drosophila melanogaster neuroligin 4, transcript variant B (Nlg4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa05459 NM_001043265.2
Latest version!
Drosophila melanogaster neuroligin 4, transcript variant C (Nlg4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa05458
    Clone ID Related Accession (Same CDS sequence) NM_001170191.2
    Accession Version NM_001170191.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neuroligin 4, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170191.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
    TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC
    CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC
    TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC
    AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG
    GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG
    CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA
    AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT
    GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA
    AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC
    TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA
    GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC
    CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC
    TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT
    CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC
    ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG
    CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG
    GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG
    CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC
    TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA
    GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA
    ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT
    TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT
    ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC
    GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG
    GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC
    CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA
    AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC
    GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC
    GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA
    AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT
    GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG
    GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT
    TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT
    TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC
    AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG
    TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC
    CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG
    GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC
    TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG
    GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT
    TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG
    ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG
    AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG
    CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG
    AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA
    GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG
    CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG
    GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG
    CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA
    ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC
    GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC
    AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT
    TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT
    CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT
    GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT
    GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGCAG AGCTGCAGCA ATATCAGCAG
    CAACAGCAGT CGAGCAAACA TCAGTCGAAT GGAGCTGTCC TCACTGGCAT GCAGTCGCAT
    CACATACGCG GACCACGCCC ACCGCTCCGC ACCGCCTCCT CGACGACAAC GAACAGCAGC
    AGCAATAACT CGGCAATGGG CGTGGCCGTG GGCGTAGGCG GGGGCGTTGG TAACATGCTC
    CTGGACCAAA CGCCATCCGG CGGCAGCAGC AGCAGCGGCG TAAGCAGCGC ACCCAGCTCC
    TCAAAGGGTC ACCACACCCA CTCGCACTCC CATGCCGCCC ACCTGGTGCA CTCGCACGGC
    GACGGCCTCG TCCTGACCTC CGCCTCGCCC GTGGGCGGCC AGCAACAGCA GCACCCGCAG
    CAGCATCAGC ATCCGCAGCA GCAGCAGCAA GTGCCGCAGG CGGCGATGGA CGAGATGCGC
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163662.1
    CDS863..4708
    Translation

    Target ORF information:

    RefSeq Version NM_001170191.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neuroligin 4, transcript variant D (Nlg4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170191.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
    TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC
    CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC
    TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC
    AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG
    GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG
    CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA
    AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT
    GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA
    AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC
    TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA
    GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC
    CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC
    TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT
    CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC
    ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG
    CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG
    GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG
    CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC
    TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA
    GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA
    ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT
    TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT
    ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC
    GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG
    GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC
    CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA
    AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC
    GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC
    GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA
    AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT
    GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG
    GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT
    TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT
    TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC
    AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG
    TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC
    CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG
    GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC
    TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG
    GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT
    TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG
    ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG
    AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG
    CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG
    AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA
    GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG
    CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG
    GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG
    CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA
    ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC
    GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC
    AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT
    TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT
    CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT
    GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT
    GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGCAG AGCTGCAGCA ATATCAGCAG
    CAACAGCAGT CGAGCAAACA TCAGTCGAAT GGAGCTGTCC TCACTGGCAT GCAGTCGCAT
    CACATACGCG GACCACGCCC ACCGCTCCGC ACCGCCTCCT CGACGACAAC GAACAGCAGC
    AGCAATAACT CGGCAATGGG CGTGGCCGTG GGCGTAGGCG GGGGCGTTGG TAACATGCTC
    CTGGACCAAA CGCCATCCGG CGGCAGCAGC AGCAGCGGCG TAAGCAGCGC ACCCAGCTCC
    TCAAAGGGTC ACCACACCCA CTCGCACTCC CATGCCGCCC ACCTGGTGCA CTCGCACGGC
    GACGGCCTCG TCCTGACCTC CGCCTCGCCC GTGGGCGGCC AGCAACAGCA GCACCCGCAG
    CAGCATCAGC ATCCGCAGCA GCAGCAGCAA GTGCCGCAGG CGGCGATGGA CGAGATGCGC
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05459
    Clone ID Related Accession (Same CDS sequence) NM_001170190.2 , NM_001043265.2 , NM_001300491.1
    Accession Version NM_001170190.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neuroligin 4, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170190.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
    TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC
    CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC
    TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC
    AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG
    GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG
    CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA
    AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT
    GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA
    AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC
    TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA
    GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC
    CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC
    TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT
    CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC
    ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG
    CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG
    GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG
    CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC
    TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA
    GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA
    ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT
    TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT
    ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC
    GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG
    GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC
    CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA
    AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC
    GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC
    GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA
    AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT
    GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG
    GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT
    TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT
    TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC
    AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG
    TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC
    CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG
    GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC
    TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG
    GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT
    TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG
    ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG
    AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG
    CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG
    AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA
    GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG
    CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG
    GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG
    CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA
    ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC
    GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC
    AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT
    TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT
    CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT
    GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT
    GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA
    CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC
    ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC
    AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG
    GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA
    AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC
    GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG
    CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163661.1
    CDS863..4705
    Translation

    Target ORF information:

    RefSeq Version NM_001170190.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neuroligin 4, transcript variant B (Nlg4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
    TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC
    CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC
    TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC
    AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG
    GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG
    CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA
    AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT
    GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA
    AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC
    TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA
    GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC
    CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC
    TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT
    CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC
    ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG
    CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG
    GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG
    CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC
    TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA
    GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA
    ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT
    TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT
    ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC
    GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG
    GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC
    CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA
    AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC
    GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC
    GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA
    AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT
    GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG
    GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT
    TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT
    TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC
    AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG
    TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC
    CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG
    GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC
    TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG
    GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT
    TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG
    ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG
    AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG
    CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG
    AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA
    GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG
    CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG
    GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG
    CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA
    ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC
    GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC
    AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT
    TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT
    CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT
    GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT
    GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA
    CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC
    ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC
    AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG
    GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA
    AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC
    GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG
    CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05459
    Clone ID Related Accession (Same CDS sequence) NM_001170190.2 , NM_001043265.2 , NM_001300491.1
    Accession Version NM_001043265.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neuroligin 4, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_001043265.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
    TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC
    CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC
    TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC
    AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG
    GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG
    CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA
    AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT
    GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA
    AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC
    TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA
    GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC
    CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC
    TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT
    CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC
    ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG
    CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG
    GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG
    CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC
    TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA
    GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA
    ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT
    TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT
    ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC
    GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG
    GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC
    CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA
    AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC
    GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC
    GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA
    AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT
    GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG
    GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT
    TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT
    TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC
    AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG
    TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC
    CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG
    GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC
    TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG
    GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT
    TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG
    ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG
    AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG
    CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG
    AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA
    GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG
    CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG
    GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG
    CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA
    ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC
    GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC
    AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT
    TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT
    CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT
    GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT
    GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA
    CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC
    ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC
    AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG
    GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA
    AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC
    GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG
    CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036730.2
    CDS882..4724
    Translation

    Target ORF information:

    RefSeq Version NM_001043265.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neuroligin 4, transcript variant C (Nlg4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001043265.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
    TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC
    CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC
    TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC
    AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG
    GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG
    CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA
    AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT
    GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA
    AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC
    TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA
    GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC
    CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC
    TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT
    CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC
    ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG
    CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG
    GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG
    CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC
    TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA
    GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA
    ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT
    TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT
    ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC
    GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG
    GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC
    CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA
    AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC
    GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC
    GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA
    AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT
    GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG
    GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT
    TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT
    TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC
    AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG
    TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC
    CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG
    GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC
    TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG
    GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT
    TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG
    ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG
    AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG
    CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG
    AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA
    GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG
    CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG
    GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG
    CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA
    ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC
    GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC
    AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT
    TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT
    CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT
    GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT
    GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA
    CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC
    ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC
    AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG
    GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA
    AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC
    GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG
    CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05459
    Clone ID Related Accession (Same CDS sequence) NM_001170190.2 , NM_001043265.2 , NM_001300491.1
    Accession Version NM_001300491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neuroligin 4, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
    TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC
    CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC
    TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC
    AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG
    GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG
    CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA
    AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT
    GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA
    AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC
    TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA
    GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC
    CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC
    TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT
    CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC
    ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG
    CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG
    GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG
    CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC
    TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA
    GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA
    ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT
    TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT
    ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC
    GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG
    GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC
    CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA
    AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC
    GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC
    GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA
    AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT
    GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG
    GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT
    TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT
    TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC
    AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG
    TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC
    CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG
    GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC
    TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG
    GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT
    TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG
    ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG
    AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG
    CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG
    AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA
    GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG
    CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG
    GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG
    CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA
    ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC
    GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC
    AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT
    TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT
    CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT
    GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT
    GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA
    CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC
    ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC
    AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG
    GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA
    AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC
    GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG
    CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287420.1
    CDS863..4705
    Translation

    Target ORF information:

    RefSeq Version NM_001300491.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neuroligin 4, transcript variant E (Nlg4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
    TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC
    CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC
    TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC
    AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG
    GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG
    CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA
    AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT
    GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA
    AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC
    TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA
    GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC
    CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC
    TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT
    CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC
    ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG
    CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG
    GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG
    CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC
    TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA
    GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA
    ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT
    TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT
    ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC
    GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG
    GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC
    CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA
    AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC
    GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC
    GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA
    AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT
    GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG
    GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT
    TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT
    TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC
    AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG
    TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC
    CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG
    GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC
    TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG
    GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT
    TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG
    ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG
    AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG
    CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG
    AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA
    GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG
    CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG
    GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG
    CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA
    ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC
    GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC
    AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT
    TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT
    CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT
    GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT
    GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA
    CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC
    ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC
    AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG
    GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA
    AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC
    GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG
    CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.