Ppat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppat
Entrez Gene ID	117544
Full Name	phosphoribosyl pyrophosphate amidotransferase
Synonyms	Atase
General protein information	Preferred Names phosphoribosyl pyrophosphate amidotransferase Names amidophosphoribosyltransferase GPAT glutamine phosphoribosylpyrophosphate amidotransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11
Summary	catalyzes the irreversible reaction between 5- phosphoribosyl-1-pyrophosphate, glutamine, and water to form 5P-phosphoribosyl-1-amine, glutamate, and inorganic pyrophosphate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_057198.2	NP_476546.1	amidophosphoribosyltransferase

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000179	XP_003436932.1
Arabidopsis thaliana (thale cress)	ASE3	NP_195599.1
Homo sapiens (human)	PPAT	NP_002694.3
Drosophila melanogaster (fruit fly)	Prat2	NP_523949.2
Arabidopsis thaliana (thale cress)	ASE2	NP_195200.1
Pan troglodytes (chimpanzee)	PPAT	XP_001140991.1
Canis lupus familiaris (dog)	PPAT	XP_854170.1
Mus musculus (house mouse)	Ppat	NP_742158.1
Drosophila melanogaster (fruit fly)	Prat	NP_524271.2
Arabidopsis thaliana (thale cress)	ASE1	NP_179247.1
Danio rerio (zebrafish)	ppat	NP_001076346.2
Xenopus tropicalis (tropical clawed frog)	ppat	NP_989313.1
	PPAT	XP_001085606.1
Bos taurus (cattle)	PPAT	NP_001095845.1
Rattus norvegicus (Norway rat)	Ppat	NP_476546.1
Gallus gallus (chicken)	PPAT	NP_001004401.1
Caenorhabditis elegans (roundworm)	CELE_T04A8.5	NP_497958.1

Gene Ontology
Bibliography

Related articles in PubMed

Pediatric neurological syndromes and inborn errors of purine metabolism.
Camici M, Micheli V, Ipata PL, Tozzi MG
Neurochemistry international56(3)367-78(2010 Feb)

Rat genomic structure of amidophosphoribosyltransferase, cDNA sequence of aminoimidazole ribonucleotide carboxylase, and cell cycle-dependent expression of these two physically linked genes.
Iwahana H, Honda S, Tsujisawa T, Takahashi Y, Adzuma K, Katashima R, Yamaoka T, Moritani M, Yoshimoto K, Itakura M
Biochimica et biophysica acta1261(3)369-80(1995 Apr)

Molecular cloning of rat amidophosphoribosyltransferase.
Iwahana H, Yamaoka T, Mizutani M, Mizusawa N, Ii S, Yoshimoto K, Itakura M
The Journal of biological chemistry268(10)7225-37(1993 Apr)

Enzymic capacities of purine de Novo and salvage pathways for nucleotide synthesis in normal and neoplastic tissues.
Natsumeda Y, Prajda N, Donohue JP, Glover JL, Weber G
Cancer research44(6)2475-9(1984 Jun)

Rapid in vivo inactivation by acivicin of CTP synthetase, carbamoyl-phosphate synthetase II, and amidophosphoribosyltransferase in hepatoma.
Denton JE, Lui MS, Aoki T, Sebolt J, Weber G
Life sciences30(13)1073-80(1982 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11577	NM_057198.2 Latest version!	Rattus norvegicus phosphoribosyl pyrophosphate amidotransferase (Ppat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppat ( NM_057198.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_476546.1 Rattus norvegicus phosphoribosyl pyrophosphate amidotransferase (Ppat), mRNA.

Price & Availability↑

CloneID	ORa11577
Clone ID Related Accession (Same CDS sequence)	NM_057198.2
Accession Version	NM_057198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	amidophosphoribosyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086999.1. On or before Oct 23, 2008 this sequence version replaced XM_001076038.1, XM_579602.2, NM_057198.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086999.1, D10853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAACTGG AGGAGTCGGG GATCCGTGAG GAATGTGGTG TGTTCGGGTG CATCGCTTCT 
GGAGACTGGC CCACGCAGCT AGATGTGCCA CATGTGATCA CTCTGGGACT CGTGGGGCTT 
CAGCACCGAG GTCAGGAGAG CGCTGGTATT GTGACCAGTG ATGGGAGTTC AGTGCCTAAA 
TTCAGGGTGC ATAAGGGAAT GGGCCTTGTA AATCATGTTT TTACCGAAGA CAATTTGAAG 
AAATTGTATG TCTCAAATCT TGGAATCGGA CATACGCGGT ACGCCACCAC AGGGAAGTGT 
GAGCTGGAAA ATTGCCAGCC GTTTGTTGTT GAGACTCTGC ATGGGAAAAT AGCTGTGGCC 
CATAACGGAG AGCTGGTGAA CGCAGCTCGG CTGAGGAAGA AGCTTCTGCG GCATGGTATT 
GGGCTTTCCA CATCCTCCGA TAGTGAAATG ATTACCCAGT TACTCGCATA TACCCCTCCT 
CAGGAACAAG ATGATACTCC AGACTGGGTA GCAAGAATTA AGAACTTAAT GAAGGAAGCG 
CCCGCAGCCT ACTCCCTGGT CATAATGCAC AGGGACGTCA TTTACGCAGT GCGAGATCCA 
TACGGAAATC GTCCTCTATG CATCGGCCGT CTTATGCCAG TTTCTGACAT AAATGACAAA 
GAGAAAAAGT CTTCAGAGAC TGAAGGATGG GTGGTGTCCT CAGAGTCTTG CAGCTTTTTA 
TCTATTGGTG CGAGATATTG CCATGAAGTG AAGCCTGGGG AAATTGTAGA AATATCCAGA 
CATGGTGTCA GAACTCTTGA TATAATTCCA AGGTCTAATG GAGACCCAGT GGCTTTTTGT 
ATCTTCGAAT ATGTTTATTT TGCAAGACCA GATAGTATGT TTGAAGACCA GATGGTTTAT 
ACGGTAAGGT ACCGGTGTGG CCAGCAGCTG GCAGTTGAAG CACCAGTGGA GGCGGACCTG 
GTCAGCACAG TTCCAGAATC TGCCACGCCT GCGGCCCTGG GGTATGCGAC AAAGTGTGGG 
CTGCCATATG TGGAGGTGCT GTGTAAGAAC CGGTATGTAG GAAGAACCTT CATTCAGCCA 
AACATGAGGC TAAGACAACT TGGGGTTGCA AAGAAATTCG GCGTGTTGTC TGACAACTTT 
AAAGGCAAAA GAATTGTTCT CATAGATGAT TCCATTGTGA GAGGCAATAC TATCTCACCC 
ATCATAAAAC TCCTCAAAGA ATCCGGTGCA AAGGAGGTAC ACATTCGAGT CGCTTCGCCA 
CCAATAAAAC ATCCGTGCTT CATGGGAATA AACATTCCTA CAAAAGAAGA GCTTATAGCC 
AATAAGCCCG AATTTGAGTA TCTTGCAGAG TACCTTGGAG CAAACAGCGT TGTGTATCTG 
TCAGTGGAAG GACTAGTTTC GTCTGTGCAA CAAGAAATAA AGTTCAAGAA GCAGAAAGTT 
AAAAAGCGTG ATATCACAAT TCAAGAAAAT GGAAATGGTC TGGAGTATTT TGAGAAGACG 
GGACACTGTA CGGCTTGCCT CACTGGCCAG TACCCTGTGG ATCTGGAATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476546.1
CDS	561..2114
Translation	MELEESGIREECGVFGCIASGDWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSSVPKFRVHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGIGLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKNLMKEAPAAYSLVIMHRDVIYAVRDPYGNRPLCIGRLMPVSDINDKEKKSSETEGWVVSSESCSFLSIGARYCHEVKPGEIVEISRHGVRTLDIIPRSNGDPVAFCIFEYVYFARPDSMFEDQMVYTVRYRCGQQLAVEAPVEADLVSTVPESATPAALGYATKCGLPYVEVLCKNRYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLIDDSIVRGNTISPIIKLLKESGAKEVHIRVASPPIKHPCFMGINIPTKEELIANKPEFEYLAEYLGANSVVYLSVEGLVSSVQQEIKFKKQKVKKRDITIQENGNGLEYFEKTGHCTACLTGQYPVDLEW

Target ORF information:

RefSeq Version	NM_057198.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoribosyl pyrophosphate amidotransferase (Ppat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057198.2

ORF Insert Sequence:

ATGGAACTGG AGGAGTCGGG GATCCGTGAG GAATGTGGTG TGTTCGGGTG CATCGCTTCT 
GGAGACTGGC CCACGCAGCT AGATGTGCCA CATGTGATCA CTCTGGGACT CGTGGGGCTT 
CAGCACCGAG GTCAGGAGAG CGCTGGTATT GTGACCAGTG ATGGGAGTTC AGTGCCTAAA 
TTCAGGGTGC ATAAGGGAAT GGGCCTTGTA AATCATGTTT TTACCGAAGA CAATTTGAAG 
AAATTGTATG TCTCAAATCT TGGAATCGGA CATACGCGGT ACGCCACCAC AGGGAAGTGT 
GAGCTGGAAA ATTGCCAGCC GTTTGTTGTT GAGACTCTGC ATGGGAAAAT AGCTGTGGCC 
CATAACGGAG AGCTGGTGAA CGCAGCTCGG CTGAGGAAGA AGCTTCTGCG GCATGGTATT 
GGGCTTTCCA CATCCTCCGA TAGTGAAATG ATTACCCAGT TACTCGCATA TACCCCTCCT 
CAGGAACAAG ATGATACTCC AGACTGGGTA GCAAGAATTA AGAACTTAAT GAAGGAAGCG 
CCCGCAGCCT ACTCCCTGGT CATAATGCAC AGGGACGTCA TTTACGCAGT GCGAGATCCA 
TACGGAAATC GTCCTCTATG CATCGGCCGT CTTATGCCAG TTTCTGACAT AAATGACAAA 
GAGAAAAAGT CTTCAGAGAC TGAAGGATGG GTGGTGTCCT CAGAGTCTTG CAGCTTTTTA 
TCTATTGGTG CGAGATATTG CCATGAAGTG AAGCCTGGGG AAATTGTAGA AATATCCAGA 
CATGGTGTCA GAACTCTTGA TATAATTCCA AGGTCTAATG GAGACCCAGT GGCTTTTTGT 
ATCTTCGAAT ATGTTTATTT TGCAAGACCA GATAGTATGT TTGAAGACCA GATGGTTTAT 
ACGGTAAGGT ACCGGTGTGG CCAGCAGCTG GCAGTTGAAG CACCAGTGGA GGCGGACCTG 
GTCAGCACAG TTCCAGAATC TGCCACGCCT GCGGCCCTGG GGTATGCGAC AAAGTGTGGG 
CTGCCATATG TGGAGGTGCT GTGTAAGAAC CGGTATGTAG GAAGAACCTT CATTCAGCCA 
AACATGAGGC TAAGACAACT TGGGGTTGCA AAGAAATTCG GCGTGTTGTC TGACAACTTT 
AAAGGCAAAA GAATTGTTCT CATAGATGAT TCCATTGTGA GAGGCAATAC TATCTCACCC 
ATCATAAAAC TCCTCAAAGA ATCCGGTGCA AAGGAGGTAC ACATTCGAGT CGCTTCGCCA 
CCAATAAAAC ATCCGTGCTT CATGGGAATA AACATTCCTA CAAAAGAAGA GCTTATAGCC 
AATAAGCCCG AATTTGAGTA TCTTGCAGAG TACCTTGGAG CAAACAGCGT TGTGTATCTG 
TCAGTGGAAG GACTAGTTTC GTCTGTGCAA CAAGAAATAA AGTTCAAGAA GCAGAAAGTT 
AAAAAGCGTG ATATCACAAT TCAAGAAAAT GGAAATGGTC TGGAGTATTT TGAGAAGACG 
GGACACTGTA CGGCTTGCCT CACTGGCCAG TACCCTGTGG ATCTGGAATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pediatric neurological syndromes and inborn errors of purine metabolism.
Neurochemistry international56(3)367-78(2010 Feb)
Camici M,Micheli V,Ipata PL,Tozzi MG

Rat genomic structure of amidophosphoribosyltransferase, cDNA sequence of aminoimidazole ribonucleotide carboxylase, and cell cycle-dependent expression of these two physically linked genes.
Biochimica et biophysica acta1261(3)369-80(1995 Apr)
Iwahana H,Honda S,Tsujisawa T,Takahashi Y,Adzuma K,Katashima R,Yamaoka T,Moritani M,Yoshimoto K,Itakura M

Molecular cloning of rat amidophosphoribosyltransferase.
The Journal of biological chemistry268(10)7225-37(1993 Apr)
Iwahana H,Yamaoka T,Mizutani M,Mizusawa N,Ii S,Yoshimoto K,Itakura M

Enzymic capacities of purine de Novo and salvage pathways for nucleotide synthesis in normal and neoplastic tissues.
Cancer research44(6)2475-9(1984 Jun)
Natsumeda Y,Prajda N,Donohue JP,Glover JL,Weber G

Rapid in vivo inactivation by acivicin of CTP synthetase, carbamoyl-phosphate synthetase II, and amidophosphoribosyltransferase in hepatoma.
Life sciences30(13)1073-80(1982 Mar)
Denton JE,Lui MS,Aoki T,Sebolt J,Weber G

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Ppat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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