Ppat cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppat
Entrez Gene ID	231327
Full Name	phosphoribosyl pyrophosphate amidotransferase
Synonyms	5730454C12Rik,AA408689,AA675351,AI507113,C79945
General protein information	Preferred Names phosphoribosyl pyrophosphate amidotransferase Names amidophosphoribosyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_172146.2	NP_742158.1	amidophosphoribosyltransferase

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000179	XP_003436932.1
Arabidopsis thaliana (thale cress)	ASE3	NP_195599.1
Homo sapiens (human)	PPAT	NP_002694.3
Drosophila melanogaster (fruit fly)	Prat2	NP_523949.2
Arabidopsis thaliana (thale cress)	ASE2	NP_195200.1
Pan troglodytes (chimpanzee)	PPAT	XP_001140991.1
Canis lupus familiaris (dog)	PPAT	XP_854170.1
Mus musculus (house mouse)	Ppat	NP_742158.1
Drosophila melanogaster (fruit fly)	Prat	NP_524271.2
Arabidopsis thaliana (thale cress)	ASE1	NP_179247.1
Danio rerio (zebrafish)	ppat	NP_001076346.2
Xenopus tropicalis (tropical clawed frog)	ppat	NP_989313.1
	PPAT	XP_001085606.1
Bos taurus (cattle)	PPAT	NP_001095845.1
Rattus norvegicus (Norway rat)	Ppat	NP_476546.1
Gallus gallus (chicken)	PPAT	NP_001004401.1
Caenorhabditis elegans (roundworm)	CELE_T04A8.5	NP_497958.1

Gene Ontology
Bibliography

Related articles in PubMed

Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models.
Kurogi S, Sekimoto T, Funamoto T, Ota T, Nakamura S, Nagai T, Nakahara M, Yoshinobu K, Araki K, Araki M, Chosa E
Scientific reports740692(2017 Jan)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Exchangeable gene trap using the Cre/mutated lox system.
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02892	NM_172146.2 Latest version!	Mus musculus phosphoribosyl pyrophosphate amidotransferase (Ppat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppat ( NM_172146.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_742158.1 Mus musculus phosphoribosyl pyrophosphate amidotransferase (Ppat), mRNA.

Price & Availability↑

CloneID	OMu02892
Clone ID Related Accession (Same CDS sequence)	NM_172146.2
Accession Version	NM_172146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-12
Organism	Mus musculus(house mouse)
Product	amidophosphoribosyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB647933.1, AK164506.1, AK147750.1, AK148034.1 and AK159859.1. On or before Jul 8, 2009 this sequence version replaced XM_001002886.2, XM_001002879.2, XM_924520.3, XM_973973.2, XM_973937.2, XM_896000.3, NM_172146.1. ##Evidence-Data-START## Transcript exon combination :: AK147750.1, AK148034.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCTGG AGGAGTTGGG GATCCGCGAG GAATGTGGTG TGTTTGGGTG CATCGCTTCT 
GGAGACTGGC CCACGCAGCT AGATGTGCCG CATGTGATCA CTCTGGGACT CGTGGGGCTA 
CAGCACCGAG GTCAGGAGAG CGCTGGTATT GTGACCAGTG ATGGGAGTGC AGTGCCTAAA 
TTCAGGGTGC ATAAGGGAAT GGGTCTTGTA AACCATGTTT TTACCGAAGA CAATTTGAAG 
AAATTGTATG ACTCAAACCT TGGAATCGGA CATACGAGGT ACGCCACCAC GGGAAAGTGT 
GAGCTGGAAA ACTGCCAGCC ATTCGTAGTT GAGACGCTTC ACGGAAAAAT AGCTGTGGCC 
CATAACGGGG AACTGGTGAA CGCCGCTCGG TTGCGGAAGA AGCTTCTGCG GCAGGGTATT 
GGGCTTTCCA CGTCCTCTGA TAGTGAAATG ATTACCCAGT TACTCGCATA CACCCCTCCT 
CAAGAAAAAG ATGATGCTCC AGACTGGGTA GCAAGAATTA AGAACTTAAT GAAAGAAGCG 
CCCGCAGCAT ACTCCCTGGT TATAATGCAC AGGGACTTCA TTTACGCGGT GCGAGATCCA 
TATGGAAATC GTCCTCTCTG CATCGGGCGT CTTATGCCAG TCTCTGATGT AAATGACAAA 
GAGAAAAAAT CCTCAGAAAC TGAAGGATGG GTGGTGTCCT CAGAGTCTTG CAGCTTTTTA 
TCCATCGGTG CCAGATATTG CCATGAAGTC AAACCCGGAG AAATTGTAGA AATATCCAGA 
CATGGTATCA GAACTCTTGA TATAATTCCA AGGTCTAACG GAGACCCAGT GGCTTTTTGT 
ATTTTTGAAT ATGTTTATTT TGCAAGACCA GATAGTATGT TTGAAGACCA AATGGTTTAT 
ACAGTAAGGT ACCGGTGTGG TCAGCAGCTG GCAATTGAAG CACCCGTGGA GGCGGACTTG 
GTTAGCACAG TTCCAGAGTC TGCTACGCCT GCAGCGCTGG GATATGCAAC AAAGTGTGGG 
CTGCCTTATG TGGAAGTGCT GTGTAAGAAC CGGTATGTAG GAAGAACCTT CATTCAACCA 
AACATGAGGT TAAGACAACT CGGTGTTGCA AAGAAGTTTG GCGTGTTGTC TGACAACTTT 
AAAGGCAAAA GAATCGTTCT CATAGATGAT TCGATTGTGA GGGGCAATAC TATCTCACCC 
ATCATAAAAC TCCTCAAAGA ATCCGGTGCA AAAGAGGTGC ACATTCGAGT CGCTTCACCA 
CCAATAAAAT ATCCGTGCTT CATGGGAATA AACATTCCTA CAAAAGAAGA GCTCATAGCC 
AATAAGCCAG AATTTGATTG TCTTGCAGAG TACCTCGGAG CAAACAGCGT TGTGTATCTG 
TCAGTGGAAG GACTGGTTTC ATCTGTGCAA CAAGAAATAA AATTTAAGAA GCAGAAAGTG 
AAAAAACATG ATATTGCAAT TCAAGAAAAT GGAAATGGTT TGGAATATTT TGAAAAGACG 
GGACACTGTA CAGCTTGCCT CACTGGGCAG TACCCTGTGG AGCTGGAATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742158.1
CDS	495..2048
Translation	MELEELGIREECGVFGCIASGDWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSAVPKFRVHKGMGLVNHVFTEDNLKKLYDSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRQGIGLSTSSDSEMITQLLAYTPPQEKDDAPDWVARIKNLMKEAPAAYSLVIMHRDFIYAVRDPYGNRPLCIGRLMPVSDVNDKEKKSSETEGWVVSSESCSFLSIGARYCHEVKPGEIVEISRHGIRTLDIIPRSNGDPVAFCIFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAPVEADLVSTVPESATPAALGYATKCGLPYVEVLCKNRYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLIDDSIVRGNTISPIIKLLKESGAKEVHIRVASPPIKYPCFMGINIPTKEELIANKPEFDCLAEYLGANSVVYLSVEGLVSSVQQEIKFKKQKVKKHDIAIQENGNGLEYFEKTGHCTACLTGQYPVELEW

Target ORF information:

RefSeq Version	NM_172146.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoribosyl pyrophosphate amidotransferase (Ppat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172146.2

ORF Insert Sequence:

ATGGAGCTGG AGGAGTTGGG GATCCGCGAG GAATGTGGTG TGTTTGGGTG CATCGCTTCT 
GGAGACTGGC CCACGCAGCT AGATGTGCCG CATGTGATCA CTCTGGGACT CGTGGGGCTA 
CAGCACCGAG GTCAGGAGAG CGCTGGTATT GTGACCAGTG ATGGGAGTGC AGTGCCTAAA 
TTCAGGGTGC ATAAGGGAAT GGGTCTTGTA AACCATGTTT TTACCGAAGA CAATTTGAAG 
AAATTGTATG ACTCAAACCT TGGAATCGGA CATACGAGGT ACGCCACCAC GGGAAAGTGT 
GAGCTGGAAA ACTGCCAGCC ATTCGTAGTT GAGACGCTTC ACGGAAAAAT AGCTGTGGCC 
CATAACGGGG AACTGGTGAA CGCCGCTCGG TTGCGGAAGA AGCTTCTGCG GCAGGGTATT 
GGGCTTTCCA CGTCCTCTGA TAGTGAAATG ATTACCCAGT TACTCGCATA CACCCCTCCT 
CAAGAAAAAG ATGATGCTCC AGACTGGGTA GCAAGAATTA AGAACTTAAT GAAAGAAGCG 
CCCGCAGCAT ACTCCCTGGT TATAATGCAC AGGGACTTCA TTTACGCGGT GCGAGATCCA 
TATGGAAATC GTCCTCTCTG CATCGGGCGT CTTATGCCAG TCTCTGATGT AAATGACAAA 
GAGAAAAAAT CCTCAGAAAC TGAAGGATGG GTGGTGTCCT CAGAGTCTTG CAGCTTTTTA 
TCCATCGGTG CCAGATATTG CCATGAAGTC AAACCCGGAG AAATTGTAGA AATATCCAGA 
CATGGTATCA GAACTCTTGA TATAATTCCA AGGTCTAACG GAGACCCAGT GGCTTTTTGT 
ATTTTTGAAT ATGTTTATTT TGCAAGACCA GATAGTATGT TTGAAGACCA AATGGTTTAT 
ACAGTAAGGT ACCGGTGTGG TCAGCAGCTG GCAATTGAAG CACCCGTGGA GGCGGACTTG 
GTTAGCACAG TTCCAGAGTC TGCTACGCCT GCAGCGCTGG GATATGCAAC AAAGTGTGGG 
CTGCCTTATG TGGAAGTGCT GTGTAAGAAC CGGTATGTAG GAAGAACCTT CATTCAACCA 
AACATGAGGT TAAGACAACT CGGTGTTGCA AAGAAGTTTG GCGTGTTGTC TGACAACTTT 
AAAGGCAAAA GAATCGTTCT CATAGATGAT TCGATTGTGA GGGGCAATAC TATCTCACCC 
ATCATAAAAC TCCTCAAAGA ATCCGGTGCA AAAGAGGTGC ACATTCGAGT CGCTTCACCA 
CCAATAAAAT ATCCGTGCTT CATGGGAATA AACATTCCTA CAAAAGAAGA GCTCATAGCC 
AATAAGCCAG AATTTGATTG TCTTGCAGAG TACCTCGGAG CAAACAGCGT TGTGTATCTG 
TCAGTGGAAG GACTGGTTTC ATCTGTGCAA CAAGAAATAA AATTTAAGAA GCAGAAAGTG 
AAAAAACATG ATATTGCAAT TCAAGAAAAT GGAAATGGTT TGGAATATTT TGAAAAGACG 
GGACACTGTA CAGCTTGCCT CACTGGGCAG TACCCTGTGG AGCTGGAATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models.
Scientific reports740692(2017 Jan)
Kurogi S,Sekimoto T,Funamoto T,Ota T,Nakamura S,Nagai T,Nakahara M,Yoshinobu K,Araki K,Araki M,Chosa E

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Exchangeable gene trap using the Cre/mutated lox system.
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)
Araki K,Imaizumi T,Sekimoto T,Yoshinobu K,Yoshimuta J,Akizuki M,Miura K,Araki M,Yamamura K

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Ppat cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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