Prat2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prat2
Entrez Gene ID	38753
Full Name	Phosphoribosylamidotransferase 2
Synonyms	CG10078,DmPrat2,Dmel\CG10078
General protein information	Preferred Names Phosphoribosylamidotransferase 2 Names CG10078 gene product from transcript CG10078-RB CG10078-PA CG10078-PB Phosphoribosylamidotransferase-2 Prat2-PA Prat2-PB amidophosphoribosyltransferase phosphoribosyl amidotransferase 2 phosphoribosylamidotransferase 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65D2-65D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079225.3	NP_523949.2	phosphoribosylamidotransferase 2, isoform B
NM_168181.2	NP_729191.1	phosphoribosylamidotransferase 2, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000179	XP_003436932.1
Arabidopsis thaliana (thale cress)	ASE3	NP_195599.1
Homo sapiens (human)	PPAT	NP_002694.3
Drosophila melanogaster (fruit fly)	Prat2	NP_523949.2
Arabidopsis thaliana (thale cress)	ASE2	NP_195200.1
Pan troglodytes (chimpanzee)	PPAT	XP_001140991.1
Canis lupus familiaris (dog)	PPAT	XP_854170.1
Mus musculus (house mouse)	Ppat	NP_742158.1
Drosophila melanogaster (fruit fly)	Prat	NP_524271.2
Arabidopsis thaliana (thale cress)	ASE1	NP_179247.1
Danio rerio (zebrafish)	ppat	NP_001076346.2
Xenopus tropicalis (tropical clawed frog)	ppat	NP_989313.1
	PPAT	XP_001085606.1
Bos taurus (cattle)	PPAT	NP_001095845.1
Rattus norvegicus (Norway rat)	Ppat	NP_476546.1
Gallus gallus (chicken)	PPAT	NP_001004401.1
Caenorhabditis elegans (roundworm)	CELE_T04A8.5	NP_497958.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prat2 is required during the prepupal stage while Prat is more important for the pupal stage; Prat2 is essential for fly development.
Title: The purine synthesis gene Prat2 is required for Drosophila metamorphosis, as revealed by inverted-repeat-mediated RNA interference.

Evidence suggests that Prat originated by gene duplication by retrotransposition of Prat2.
Title: The PRAT purine synthesis gene duplication in Drosophila melanogaster and Drosophila virilis is associated with a retrotransposition event and diversification of expression patterns.

The following Prat2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prat2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17726	NM_079225.3 Latest version!	Drosophila melanogaster phosphoribosylamidotransferase 2, transcript variant B (Prat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17727	NM_168181.2 Latest version!	Drosophila melanogaster phosphoribosylamidotransferase 2, transcript variant A (Prat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prat2 ( NM_079225.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523949.2 Drosophila melanogaster phosphoribosylamidotransferase 2, transcript variant B (Prat2), mRNA.

Price & Availability↑

CloneID	OFa17726
Clone ID Related Accession (Same CDS sequence)	NM_079225.3
Accession Version	NM_079225.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphoribosylamidotransferase 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079225.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGCGC AAAGTGATTC TGGAAATTGC TGTGCCAAGC TTAATAAAGA TGAGTGCTGC 
CAGAAGACAA AGGATGTGAT CTTGAGCGGC GTATCTCCCA AAATGTTTCC CGACAAAATC 
TACAAGTCTG TTCAACCCAA AGGAGAGGAT GCCACCGGAA TGACCTGCGA ATGCGGTGTT 
TTTGCAGCCA TCGCTTGCGG GGATTACCCA ACACAACTGG ACATAGCACA GATGATCTGC 
CTCGGTCTGG TGGCTCTGCA GCATCGTGGC CAGGAGTCGG CGGGAATAGT GACCAGTTTG 
GGCAAGTCCT CCAAGAATTT CAGCGTACAC AAGGGCATGG GCATGATCAA CAATCTGTTC 
AATGACGAGG CGATCAGGAA GTTAAAGGGT AACCTGGGAA TAGGACACAC CCGGTATTCC 
ACGGCCGCAG CCTCGGAAGT TGTGAACTGC CAGCCGTTTG TGGTGCACAC TGCCCATGGA 
GCACTGGCCA TTGCCCACAA TGGTGAGCTA GTGAACTGCG AGTCCCTGAG GAGGGAGGTC 
CTGGAGCGAG GAGTGGGCCT GTCTACGCAC AGTGACAGTG AACTTATTGC CCAATCCCTG 
TGCTGTGCAC CAGAGGATGT GTCGGAGCAC GATGGACCCA ATTGGCCGGC CAGAATTCGC 
CATTTCATGA CCCTGGCTCC ACTCTCCTAC TCCCTGGTCG TGATGCACAA GGATAAGATC 
TACGCTGTCC GCGATTCCTA TGGCAATAGA CCGCTGTGCT TGGGAAAAAT CGTGCCCGTT 
GATGCAGGAC ATGCGAATAT CAATGACCAA CTGGCCGAGG GTTGGGTGGT CAGTAGTGAA 
AGCTGCGGAT TCCTCTCCAT CGGAGCCAGA TATGTTCGCG AGGTGGAGCC CGGTGAGATC 
ATCGAGCTGT CACGAAATGG TTATAGAACC GTGGATATCG TTGAAAGACC GGACTATAAG 
CGCATGGCAT TCTGCATCTT TGAGTACGTC TACTTTGCCC GCAGTGATTC CATGTTCGAG 
GGTCAGATGG TTTACTCAGC CCGCCTGCAA TGTGGTCGCC AGTTGGCCAG GGAATCGCCA 
TTAGATGCGG ATTTGGTCAG CTCGGTTCCG GAATCGGGCA CAGCAGCTGC CCATGGTTAT 
GCCAGGGAGT CGGGACTGCC ATTCGGGGAA GTTCTCTGCA AGAATCGCTA TGTGGGCCGC 
ACGTTCATCC AACCCTCGAC GAGATTGCGA CAGTTGGGCG TGGCCAAAAA GTTTGGTGCC 
CTGGCTCAAA ATGTCGAGGG CAAGCGGATC GTGCTAGTCG ATGACTCCAT AGTGAGGGGA 
AATACCATTG GACCGATTAT CAAACTCCTG CGAGATGCCG GCGCCACCGA GGTGCACATT 
CGCATTGCCA GTCCTCCGCT GCAATATCCC TGCTACATGG GTATCAATAT ACCCACTCGT 
GAGGAGCTAA TAGCCAACAA ATTGAATGCG GATCAGCTGG CGGATCACGT GGGTGCGGAT 
AGTCTGGCCT ATTTGAGTGT GGAGGGTCTG GTCAAGGCCG TCCAGATGAA CAAGGCCCAT 
GTGAATCCAC TCAAAGCTGG CTACTGCACC GCTTGCCTAA CGGGCGAGTA TCCTGGCGGA 
TTGCCGGAGG AGCTCAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523949.2
CDS	188..1831
Translation	MMAQSDSGNCCAKLNKDECCQKTKDVILSGVSPKMFPDKIYKSVQPKGEDATGMTCECGVFAAIACGDYPTQLDIAQMICLGLVALQHRGQESAGIVTSLGKSSKNFSVHKGMGMINNLFNDEAIRKLKGNLGIGHTRYSTAAASEVVNCQPFVVHTAHGALAIAHNGELVNCESLRREVLERGVGLSTHSDSELIAQSLCCAPEDVSEHDGPNWPARIRHFMTLAPLSYSLVVMHKDKIYAVRDSYGNRPLCLGKIVPVDAGHANINDQLAEGWVVSSESCGFLSIGARYVREVEPGEIIELSRNGYRTVDIVERPDYKRMAFCIFEYVYFARSDSMFEGQMVYSARLQCGRQLARESPLDADLVSSVPESGTAAAHGYARESGLPFGEVLCKNRYVGRTFIQPSTRLRQLGVAKKFGALAQNVEGKRIVLVDDSIVRGNTIGPIIKLLRDAGATEVHIRIASPPLQYPCYMGINIPTREELIANKLNADQLADHVGADSLAYLSVEGLVKAVQMNKAHVNPLKAGYCTACLTGEYPGGLPEELSW

Target ORF information:

RefSeq Version	NM_079225.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphoribosylamidotransferase 2, transcript variant B (Prat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079225.3

ORF Insert Sequence:

ATGATGGCGC AAAGTGATTC TGGAAATTGC TGTGCCAAGC TTAATAAAGA TGAGTGCTGC 
CAGAAGACAA AGGATGTGAT CTTGAGCGGC GTATCTCCCA AAATGTTTCC CGACAAAATC 
TACAAGTCTG TTCAACCCAA AGGAGAGGAT GCCACCGGAA TGACCTGCGA ATGCGGTGTT 
TTTGCAGCCA TCGCTTGCGG GGATTACCCA ACACAACTGG ACATAGCACA GATGATCTGC 
CTCGGTCTGG TGGCTCTGCA GCATCGTGGC CAGGAGTCGG CGGGAATAGT GACCAGTTTG 
GGCAAGTCCT CCAAGAATTT CAGCGTACAC AAGGGCATGG GCATGATCAA CAATCTGTTC 
AATGACGAGG CGATCAGGAA GTTAAAGGGT AACCTGGGAA TAGGACACAC CCGGTATTCC 
ACGGCCGCAG CCTCGGAAGT TGTGAACTGC CAGCCGTTTG TGGTGCACAC TGCCCATGGA 
GCACTGGCCA TTGCCCACAA TGGTGAGCTA GTGAACTGCG AGTCCCTGAG GAGGGAGGTC 
CTGGAGCGAG GAGTGGGCCT GTCTACGCAC AGTGACAGTG AACTTATTGC CCAATCCCTG 
TGCTGTGCAC CAGAGGATGT GTCGGAGCAC GATGGACCCA ATTGGCCGGC CAGAATTCGC 
CATTTCATGA CCCTGGCTCC ACTCTCCTAC TCCCTGGTCG TGATGCACAA GGATAAGATC 
TACGCTGTCC GCGATTCCTA TGGCAATAGA CCGCTGTGCT TGGGAAAAAT CGTGCCCGTT 
GATGCAGGAC ATGCGAATAT CAATGACCAA CTGGCCGAGG GTTGGGTGGT CAGTAGTGAA 
AGCTGCGGAT TCCTCTCCAT CGGAGCCAGA TATGTTCGCG AGGTGGAGCC CGGTGAGATC 
ATCGAGCTGT CACGAAATGG TTATAGAACC GTGGATATCG TTGAAAGACC GGACTATAAG 
CGCATGGCAT TCTGCATCTT TGAGTACGTC TACTTTGCCC GCAGTGATTC CATGTTCGAG 
GGTCAGATGG TTTACTCAGC CCGCCTGCAA TGTGGTCGCC AGTTGGCCAG GGAATCGCCA 
TTAGATGCGG ATTTGGTCAG CTCGGTTCCG GAATCGGGCA CAGCAGCTGC CCATGGTTAT 
GCCAGGGAGT CGGGACTGCC ATTCGGGGAA GTTCTCTGCA AGAATCGCTA TGTGGGCCGC 
ACGTTCATCC AACCCTCGAC GAGATTGCGA CAGTTGGGCG TGGCCAAAAA GTTTGGTGCC 
CTGGCTCAAA ATGTCGAGGG CAAGCGGATC GTGCTAGTCG ATGACTCCAT AGTGAGGGGA 
AATACCATTG GACCGATTAT CAAACTCCTG CGAGATGCCG GCGCCACCGA GGTGCACATT 
CGCATTGCCA GTCCTCCGCT GCAATATCCC TGCTACATGG GTATCAATAT ACCCACTCGT 
GAGGAGCTAA TAGCCAACAA ATTGAATGCG GATCAGCTGG CGGATCACGT GGGTGCGGAT 
AGTCTGGCCT ATTTGAGTGT GGAGGGTCTG GTCAAGGCCG TCCAGATGAA CAAGGCCCAT 
GTGAATCCAC TCAAAGCTGG CTACTGCACC GCTTGCCTAA CGGGCGAGTA TCCTGGCGGA 
TTGCCGGAGG AGCTCAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prat2 ( NM_168181.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729191.1 Drosophila melanogaster phosphoribosylamidotransferase 2, transcript variant A (Prat2), mRNA.

Price & Availability↑

CloneID	OFa17727
Clone ID Related Accession (Same CDS sequence)	NM_168181.2
Accession Version	NM_168181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphoribosylamidotransferase 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168181.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTGCC TCGGTCTGGT GGCTCTGCAG CATCGTGGCC AGGAGTCGGC GGGAATAGTG 
ACCAGTTTGG GCAAGTCCTC CAAGAATTTC AGCGTACACA AGGGCATGGG CATGATCAAC 
AATCTGTTCA ATGACGAGGC GATCAGGAAG TTAAAGGGTA ACCTGGGAAT AGGACACACC 
CGGTATTCCA CGGCCGCAGC CTCGGAAGTT GTGAACTGCC AGCCGTTTGT GGTGCACACT 
GCCCATGGAG CACTGGCCAT TGCCCACAAT GGTGAGCTAG TGAACTGCGA GTCCCTGAGG 
AGGGAGGTCC TGGAGCGAGG AGTGGGCCTG TCTACGCACA GTGACAGTGA ACTTATTGCC 
CAATCCCTGT GCTGTGCACC AGAGGATGTG TCGGAGCACG ATGGACCCAA TTGGCCGGCC 
AGAATTCGCC ATTTCATGAC CCTGGCTCCA CTCTCCTACT CCCTGGTCGT GATGCACAAG 
GATAAGATCT ACGCTGTCCG CGATTCCTAT GGCAATAGAC CGCTGTGCTT GGGAAAAATC 
GTGCCCGTTG ATGCAGGACA TGCGAATATC AATGACCAAC TGGCCGAGGG TTGGGTGGTC 
AGTAGTGAAA GCTGCGGATT CCTCTCCATC GGAGCCAGAT ATGTTCGCGA GGTGGAGCCC 
GGTGAGATCA TCGAGCTGTC ACGAAATGGT TATAGAACCG TGGATATCGT TGAAAGACCG 
GACTATAAGC GCATGGCATT CTGCATCTTT GAGTACGTCT ACTTTGCCCG CAGTGATTCC 
ATGTTCGAGG GTCAGATGGT TTACTCAGCC CGCCTGCAAT GTGGTCGCCA GTTGGCCAGG 
GAATCGCCAT TAGATGCGGA TTTGGTCAGC TCGGTTCCGG AATCGGGCAC AGCAGCTGCC 
CATGGTTATG CCAGGGAGTC GGGACTGCCA TTCGGGGAAG TTCTCTGCAA GAATCGCTAT 
GTGGGCCGCA CGTTCATCCA ACCCTCGACG AGATTGCGAC AGTTGGGCGT GGCCAAAAAG 
TTTGGTGCCC TGGCTCAAAA TGTCGAGGGC AAGCGGATCG TGCTAGTCGA TGACTCCATA 
GTGAGGGGAA ATACCATTGG ACCGATTATC AAACTCCTGC GAGATGCCGG CGCCACCGAG 
GTGCACATTC GCATTGCCAG TCCTCCGCTG CAATATCCCT GCTACATGGG TATCAATATA 
CCCACTCGTG AGGAGCTAAT AGCCAACAAA TTGAATGCGG ATCAGCTGGC GGATCACGTG 
GGTGCGGATA GTCTGGCCTA TTTGAGTGTG GAGGGTCTGG TCAAGGCCGT CCAGATGAAC 
AAGGCCCATG TGAATCCACT CAAAGCTGGC TACTGCACCG CTTGCCTAAC GGGCGAGTAT 
CCTGGCGGAT TGCCGGAGGA GCTCAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729191.1
CDS	224..1636
Translation	MICLGLVALQHRGQESAGIVTSLGKSSKNFSVHKGMGMINNLFNDEAIRKLKGNLGIGHTRYSTAAASEVVNCQPFVVHTAHGALAIAHNGELVNCESLRREVLERGVGLSTHSDSELIAQSLCCAPEDVSEHDGPNWPARIRHFMTLAPLSYSLVVMHKDKIYAVRDSYGNRPLCLGKIVPVDAGHANINDQLAEGWVVSSESCGFLSIGARYVREVEPGEIIELSRNGYRTVDIVERPDYKRMAFCIFEYVYFARSDSMFEGQMVYSARLQCGRQLARESPLDADLVSSVPESGTAAAHGYARESGLPFGEVLCKNRYVGRTFIQPSTRLRQLGVAKKFGALAQNVEGKRIVLVDDSIVRGNTIGPIIKLLRDAGATEVHIRIASPPLQYPCYMGINIPTREELIANKLNADQLADHVGADSLAYLSVEGLVKAVQMNKAHVNPLKAGYCTACLTGEYPGGLPEELSW

Target ORF information:

RefSeq Version	NM_168181.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphoribosylamidotransferase 2, transcript variant A (Prat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168181.2

ORF Insert Sequence:

ATGATCTGCC TCGGTCTGGT GGCTCTGCAG CATCGTGGCC AGGAGTCGGC GGGAATAGTG 
ACCAGTTTGG GCAAGTCCTC CAAGAATTTC AGCGTACACA AGGGCATGGG CATGATCAAC 
AATCTGTTCA ATGACGAGGC GATCAGGAAG TTAAAGGGTA ACCTGGGAAT AGGACACACC 
CGGTATTCCA CGGCCGCAGC CTCGGAAGTT GTGAACTGCC AGCCGTTTGT GGTGCACACT 
GCCCATGGAG CACTGGCCAT TGCCCACAAT GGTGAGCTAG TGAACTGCGA GTCCCTGAGG 
AGGGAGGTCC TGGAGCGAGG AGTGGGCCTG TCTACGCACA GTGACAGTGA ACTTATTGCC 
CAATCCCTGT GCTGTGCACC AGAGGATGTG TCGGAGCACG ATGGACCCAA TTGGCCGGCC 
AGAATTCGCC ATTTCATGAC CCTGGCTCCA CTCTCCTACT CCCTGGTCGT GATGCACAAG 
GATAAGATCT ACGCTGTCCG CGATTCCTAT GGCAATAGAC CGCTGTGCTT GGGAAAAATC 
GTGCCCGTTG ATGCAGGACA TGCGAATATC AATGACCAAC TGGCCGAGGG TTGGGTGGTC 
AGTAGTGAAA GCTGCGGATT CCTCTCCATC GGAGCCAGAT ATGTTCGCGA GGTGGAGCCC 
GGTGAGATCA TCGAGCTGTC ACGAAATGGT TATAGAACCG TGGATATCGT TGAAAGACCG 
GACTATAAGC GCATGGCATT CTGCATCTTT GAGTACGTCT ACTTTGCCCG CAGTGATTCC 
ATGTTCGAGG GTCAGATGGT TTACTCAGCC CGCCTGCAAT GTGGTCGCCA GTTGGCCAGG 
GAATCGCCAT TAGATGCGGA TTTGGTCAGC TCGGTTCCGG AATCGGGCAC AGCAGCTGCC 
CATGGTTATG CCAGGGAGTC GGGACTGCCA TTCGGGGAAG TTCTCTGCAA GAATCGCTAT 
GTGGGCCGCA CGTTCATCCA ACCCTCGACG AGATTGCGAC AGTTGGGCGT GGCCAAAAAG 
TTTGGTGCCC TGGCTCAAAA TGTCGAGGGC AAGCGGATCG TGCTAGTCGA TGACTCCATA 
GTGAGGGGAA ATACCATTGG ACCGATTATC AAACTCCTGC GAGATGCCGG CGCCACCGAG 
GTGCACATTC GCATTGCCAG TCCTCCGCTG CAATATCCCT GCTACATGGG TATCAATATA 
CCCACTCGTG AGGAGCTAAT AGCCAACAAA TTGAATGCGG ATCAGCTGGC GGATCACGTG 
GGTGCGGATA GTCTGGCCTA TTTGAGTGTG GAGGGTCTGG TCAAGGCCGT CCAGATGAAC 
AAGGCCCATG TGAATCCACT CAAAGCTGGC TACTGCACCG CTTGCCTAAC GGGCGAGTAT 
CCTGGCGGAT TGCCGGAGGA GCTCAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Prat2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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