Dync1i2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dync1i2
Entrez Gene ID	116659
Full Name	dynein cytoplasmic 1 intermediate chain 2
Synonyms	Dnci2,Dncic2
General protein information	Preferred Names dynein cytoplasmic 1 intermediate chain 2 Names cytoplasmic dynein 1 intermediate chain 2 DH IC-2 cytoplasmic dynein intermediate chain 2 dynein intermediate chain 2, cytosolic dynein, cytoplasmic, intermediate chain 2 dynein, cytoplasmic, intermediate polypeptide 2 oncofetal protein (OFP)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q22
Summary	intermediate chain of cytoplasmic dynein, a retrograde microtubule motor thought to be involved in organelle transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591432.1	XP_017446921.1	cytoplasmic dynein 1 intermediate chain 2 isoform X1
XM_006234346.3	XP_006234408.1	cytoplasmic dynein 1 intermediate chain 2 isoform X3
NM_001270625.1	NP_001257554.1	cytoplasmic dynein 1 intermediate chain 2 isoform 3
NM_001270624.1	NP_001257553.1	cytoplasmic dynein 1 intermediate chain 2 isoform 2
NM_053880.2	NP_446332.2	cytoplasmic dynein 1 intermediate chain 2 isoform 1
XM_006234344.3	XP_006234406.1	cytoplasmic dynein 1 intermediate chain 2 isoform X2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	dyci-1	NP_501038.1
Magnaporthe oryzae (rice blast fungus)	MGG_04771	XP_003713723.1
Drosophila melanogaster (fruit fly)	Sdic1	NP_524670.2
Xenopus tropicalis (tropical clawed frog)	dync1i2	NP_998848.1
	DYNC1I2	XP_002798988.1
Canis lupus familiaris (dog)	DYNC1I2	XP_005640358.1
Rattus norvegicus (Norway rat)	Dync1i2	NP_446332.2
Danio rerio (zebrafish)	LOC100333136	XP_002662315.2
Danio rerio (zebrafish)	dync1i2b	XP_690115.6
Drosophila melanogaster (fruit fly)	sw	NP_477074.1
Drosophila melanogaster (fruit fly)	Sdic3	NP_728323.2
Mus musculus (house mouse)	Dync1i2	NP_001185803.1
Drosophila melanogaster (fruit fly)	Sdic2	NP_996516.1
Drosophila melanogaster (fruit fly)	Sdic4	NP_996519.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000320	XP_003436968.1
Neurospora crassa	NCU09142	XP_958914.1

Gene Ontology
Bibliography

Related articles in PubMed

Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84.
Blasier KR, Humsi MK, Ha J, Ross MW, Smiley WR, Inamdar NA, Mitchell DJ, Lo KW, Pfister KK
Journal of neuroscience research92(9)1143-54(2014 Sep)

Integrated proteomics identified novel activation of dynein IC2-GR-COX-1 signaling in neurofibromatosis type I (NF1) disease model cells.
Hirayama M, Kobayashi D, Mizuguchi S, Morikawa T, Nagayama M, Midorikawa U, Wilson MM, Nambu AN, Yoshizawa AC, Kawano S, Araki N
Molecular & cellular proteomics : MCP12(5)1377-94(2013 May)

Cytoplasmic dynein.
Allan VJ
Biochemical Society transactions39(5)1169-78(2011 Oct)

PEX14 is required for microtubule-based peroxisome motility in human cells.
Bharti P, Schliebs W, Schievelbusch T, Neuhaus A, David C, Kock K, Herrmann C, Meyer HE, Wiese S, Warscheid B, Theiss C, Erdmann R
Journal of cell science124(Pt 10)1759-68(2011 May)

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS
The EMBO journal30(8)1520-35(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data of this study suggest that the IC-2C S84 has a role in modulating dynein properties.
Title: Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84.

Neurofibromatosis type I-related neural abnormalities are, in part, caused by up-regulation of dynein IC2-glucocorticoid receptor-COX-1 signaling.
Title: Integrated proteomics identified novel activation of dynein IC2-GR-COX-1 signaling in neurofibromatosis type I (NF1) disease model cells.

The following Dync1i2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dync1i2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09416	NM_001270624.1 Latest version!	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa09416	XM_006234344.3 Latest version!	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa09421	NM_001270625.1 Latest version!	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa10942	NM_053880.2 Latest version!	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa09421	XM_006234346.3 Latest version!	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa10942	XM_017591432.1 Latest version!	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dync1i2 ( NM_001270624.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257553.1 Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa09416
Clone ID Related Accession (Same CDS sequence)	NM_001270624.1 , XM_006234344.3
Accession Version	NM_001270624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic dynein 1 intermediate chain 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB733957.1, FM110655.1, AC107446.5, BC078764.1 and FM076575.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: U39045.1, BC078764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTCCC TCCTCCCATG 
TCTCCATCCT CCAAGTCGGT GAGCACGCCA AGTGAAGCTG GAAGCCAGGA CTCTGGAGAT 
GGCGCCGTGG GATCTAGGAC GCTGCACTGG GATACAGATC CATCAGCTCT TCAGCTCCAC 
TCAGATTCCG ATTTGGGACG AGGACCTATT AAACTTGGAA TGGCCAAAAT TACTCAAGTC 
GACTTTCCCC CTCGAGAAAT CGTCACATAT ACAAAGGAAA CTCAGACCCC GGTTACAGCT 
CAACCCAAAG AAGATGAGGA GGAGGAAGAT GACGTAGCAG CTCCTAAGCC TCCTGTTGAA 
CCTGAAGAAG AGAAAATTTT AAAAAAAGAC GAAGAGAATG ATAGCAAAGC TCCCCCTCAT 
GAACTAACTG AAGAAGAAAA GCAACAAATT TTACACTCAG AAGAATTTTT AAGTTTCTTT 
GACCATTCTA CAAGAATTGT AGAAAGAGCC CTCTCTGAGC AGATTAACAT CTTCTTTGAC 
TACAGTGGGA GAGATTTGGA AGACAAAGAA GGGGAGATTC AAGCAGGTGC TAAGCTGTCA 
TTAAATCGAC AGTTTTTTGA TGAGCGTTGG TCAAAGCATC GAGTTGTTAG TTGCTTGGAT 
TGGTCATCCC AGTATCCAGA ATTACTTGTG GCTTCCTATA ATAACAATGA AGAGGCTCCT 
CATGAGCCTG ATGGTGTGGC CCTCGTATGG AATATGAAGT ACAAGAAAAC TACCCCAGAG 
TACGTGTTCC ACTGCCAGTC AGCTGTAATG TCTGCTACAT TTGCAAAATT TCATCCAAAT 
CTTGTTGTTG GAGGTACATA CTCAGGCCAA ATTGTGCTTT GGGATAACCG TAGCAATAAA 
AGAACCCCCG TCCAGAGAAC ACCACTGTCA GCTGCTGCAC ACACACACCC TGTCTACTGT 
GTAAATGTTG TTGGAACACA AAATGCTCAT AATCTGATTA GCATCTCTAC TGATGGAAAA 
ATTTGCTCCT GGAGCTTAGA CATGCTTTCC CATCCACAGG ATAGCATGGA GTTGGTTCAT 
AAACAGTCAA AGGCAGTAGC TGTAACATCT ATGTCTTTCC CTGTTGGAGA CGTCAACAAC 
TTTGTTGTGG GCAGTGAAGA AGGTTCTGTG TATACAGCAT GCCGCCATGG CAGCAAAGCC 
GGAATCAGTG AGATGTTTGA GGGACACCAG GGACCCATCA CGGGAATCCA CTGTCACGCA 
GCTGTTGGCG CAGTAGACTT CTCACATCTG TTCGTCACTT CGTCTTTTGA CTGGACAGTA 
AAACTTTGGA CAACTAAGAA TAACAAGCCT TTGTACTCAT TTGAAGATAA TTCAGACTAT 
GTCTATGATG TTATGTGGTC ACCCACCCAC CCAGCCCTGT TTGCCTGTGT GGATGGCATG 
GGGAGACTGG ATCTATGGAA TCTCAACAAT GACACAGAGG TGCCGACTGC CAGCATTTCT 
GTGGAGGGCA ACCCTGCCCT TAACCGTGTG AGATGGACCC ACTCGGGAAG AGAGATCGCC 
GTGGGTGATT CTGAAGGACA GATTGTCATA TATGATGTGG GCGAGCAGAT CGCTGTTCCC 
CGAAATGATG AATGGGCAAG ATTTGGCCGA ACACTCGCAG AGATTAACGC AAGCCGAGCT 
GATGCAGAGG AGGAAGCGGC TACCCGGATT CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257553.1
CDS	355..2253
Translation	MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAAVSVQEESDLEKKRREAEALLQSMGLTTDSPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRTLHWDTDPSALQLHSDSDLGRGPIKLGMAKITQVDFPPREIVTYTKETQTPVTAQPKEDEEEEDDVAAPKPPVEPEEEKILKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEEAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNSDYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINASRADAEEEAATRIPA

Target ORF information:

RefSeq Version	NM_001270624.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270624.1

ORF Insert Sequence:

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTCCC TCCTCCCATG 
TCTCCATCCT CCAAGTCGGT GAGCACGCCA AGTGAAGCTG GAAGCCAGGA CTCTGGAGAT 
GGCGCCGTGG GATCTAGGAC GCTGCACTGG GATACAGATC CATCAGCTCT TCAGCTCCAC 
TCAGATTCCG ATTTGGGACG AGGACCTATT AAACTTGGAA TGGCCAAAAT TACTCAAGTC 
GACTTTCCCC CTCGAGAAAT CGTCACATAT ACAAAGGAAA CTCAGACCCC GGTTACAGCT 
CAACCCAAAG AAGATGAGGA GGAGGAAGAT GACGTAGCAG CTCCTAAGCC TCCTGTTGAA 
CCTGAAGAAG AGAAAATTTT AAAAAAAGAC GAAGAGAATG ATAGCAAAGC TCCCCCTCAT 
GAACTAACTG AAGAAGAAAA GCAACAAATT TTACACTCAG AAGAATTTTT AAGTTTCTTT 
GACCATTCTA CAAGAATTGT AGAAAGAGCC CTCTCTGAGC AGATTAACAT CTTCTTTGAC 
TACAGTGGGA GAGATTTGGA AGACAAAGAA GGGGAGATTC AAGCAGGTGC TAAGCTGTCA 
TTAAATCGAC AGTTTTTTGA TGAGCGTTGG TCAAAGCATC GAGTTGTTAG TTGCTTGGAT 
TGGTCATCCC AGTATCCAGA ATTACTTGTG GCTTCCTATA ATAACAATGA AGAGGCTCCT 
CATGAGCCTG ATGGTGTGGC CCTCGTATGG AATATGAAGT ACAAGAAAAC TACCCCAGAG 
TACGTGTTCC ACTGCCAGTC AGCTGTAATG TCTGCTACAT TTGCAAAATT TCATCCAAAT 
CTTGTTGTTG GAGGTACATA CTCAGGCCAA ATTGTGCTTT GGGATAACCG TAGCAATAAA 
AGAACCCCCG TCCAGAGAAC ACCACTGTCA GCTGCTGCAC ACACACACCC TGTCTACTGT 
GTAAATGTTG TTGGAACACA AAATGCTCAT AATCTGATTA GCATCTCTAC TGATGGAAAA 
ATTTGCTCCT GGAGCTTAGA CATGCTTTCC CATCCACAGG ATAGCATGGA GTTGGTTCAT 
AAACAGTCAA AGGCAGTAGC TGTAACATCT ATGTCTTTCC CTGTTGGAGA CGTCAACAAC 
TTTGTTGTGG GCAGTGAAGA AGGTTCTGTG TATACAGCAT GCCGCCATGG CAGCAAAGCC 
GGAATCAGTG AGATGTTTGA GGGACACCAG GGACCCATCA CGGGAATCCA CTGTCACGCA 
GCTGTTGGCG CAGTAGACTT CTCACATCTG TTCGTCACTT CGTCTTTTGA CTGGACAGTA 
AAACTTTGGA CAACTAAGAA TAACAAGCCT TTGTACTCAT TTGAAGATAA TTCAGACTAT 
GTCTATGATG TTATGTGGTC ACCCACCCAC CCAGCCCTGT TTGCCTGTGT GGATGGCATG 
GGGAGACTGG ATCTATGGAA TCTCAACAAT GACACAGAGG TGCCGACTGC CAGCATTTCT 
GTGGAGGGCA ACCCTGCCCT TAACCGTGTG AGATGGACCC ACTCGGGAAG AGAGATCGCC 
GTGGGTGATT CTGAAGGACA GATTGTCATA TATGATGTGG GCGAGCAGAT CGCTGTTCCC 
CGAAATGATG AATGGGCAAG ATTTGGCCGA ACACTCGCAG AGATTAACGC AAGCCGAGCT 
GATGCAGAGG AGGAAGCGGC TACCCGGATT CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dync1i2 ( XM_006234344.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234406.1 Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa09416
Clone ID Related Accession (Same CDS sequence)	NM_001270624.1 , XM_006234344.3
Accession Version	XM_006234344.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic dynein 1 intermediate chain 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234344.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTCCC TCCTCCCATG 
TCTCCATCCT CCAAGTCGGT GAGCACGCCA AGTGAAGCTG GAAGCCAGGA CTCTGGAGAT 
GGCGCCGTGG GATCTAGGAC GCTGCACTGG GATACAGATC CATCAGCTCT TCAGCTCCAC 
TCAGATTCCG ATTTGGGACG AGGACCTATT AAACTTGGAA TGGCCAAAAT TACTCAAGTC 
GACTTTCCCC CTCGAGAAAT CGTCACATAT ACAAAGGAAA CTCAGACCCC GGTTACAGCT 
CAACCCAAAG AAGATGAGGA GGAGGAAGAT GACGTAGCAG CTCCTAAGCC TCCTGTTGAA 
CCTGAAGAAG AGAAAATTTT AAAAAAAGAC GAAGAGAATG ATAGCAAAGC TCCCCCTCAT 
GAACTAACTG AAGAAGAAAA GCAACAAATT TTACACTCAG AAGAATTTTT AAGTTTCTTT 
GACCATTCTA CAAGAATTGT AGAAAGAGCC CTCTCTGAGC AGATTAACAT CTTCTTTGAC 
TACAGTGGGA GAGATTTGGA AGACAAAGAA GGGGAGATTC AAGCAGGTGC TAAGCTGTCA 
TTAAATCGAC AGTTTTTTGA TGAGCGTTGG TCAAAGCATC GAGTTGTTAG TTGCTTGGAT 
TGGTCATCCC AGTATCCAGA ATTACTTGTG GCTTCCTATA ATAACAATGA AGAGGCTCCT 
CATGAGCCTG ATGGTGTGGC CCTCGTATGG AATATGAAGT ACAAGAAAAC TACCCCAGAG 
TACGTGTTCC ACTGCCAGTC AGCTGTAATG TCTGCTACAT TTGCAAAATT TCATCCAAAT 
CTTGTTGTTG GAGGTACATA CTCAGGCCAA ATTGTGCTTT GGGATAACCG TAGCAATAAA 
AGAACCCCCG TCCAGAGAAC ACCACTGTCA GCTGCTGCAC ACACACACCC TGTCTACTGT 
GTAAATGTTG TTGGAACACA AAATGCTCAT AATCTGATTA GCATCTCTAC TGATGGAAAA 
ATTTGCTCCT GGAGCTTAGA CATGCTTTCC CATCCACAGG ATAGCATGGA GTTGGTTCAT 
AAACAGTCAA AGGCAGTAGC TGTAACATCT ATGTCTTTCC CTGTTGGAGA CGTCAACAAC 
TTTGTTGTGG GCAGTGAAGA AGGTTCTGTG TATACAGCAT GCCGCCATGG CAGCAAAGCC 
GGAATCAGTG AGATGTTTGA GGGACACCAG GGACCCATCA CGGGAATCCA CTGTCACGCA 
GCTGTTGGCG CAGTAGACTT CTCACATCTG TTCGTCACTT CGTCTTTTGA CTGGACAGTA 
AAACTTTGGA CAACTAAGAA TAACAAGCCT TTGTACTCAT TTGAAGATAA TTCAGACTAT 
GTCTATGATG TTATGTGGTC ACCCACCCAC CCAGCCCTGT TTGCCTGTGT GGATGGCATG 
GGGAGACTGG ATCTATGGAA TCTCAACAAT GACACAGAGG TGCCGACTGC CAGCATTTCT 
GTGGAGGGCA ACCCTGCCCT TAACCGTGTG AGATGGACCC ACTCGGGAAG AGAGATCGCC 
GTGGGTGATT CTGAAGGACA GATTGTCATA TATGATGTGG GCGAGCAGAT CGCTGTTCCC 
CGAAATGATG AATGGGCAAG ATTTGGCCGA ACACTCGCAG AGATTAACGC AAGCCGAGCT 
GATGCAGAGG AGGAAGCGGC TACCCGGATT CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234406.1
CDS	143..2041
Translation	MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAAVSVQEESDLEKKRREAEALLQSMGLTTDSPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRTLHWDTDPSALQLHSDSDLGRGPIKLGMAKITQVDFPPREIVTYTKETQTPVTAQPKEDEEEEDDVAAPKPPVEPEEEKILKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEEAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNSDYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINASRADAEEEAATRIPA

Target ORF information:

RefSeq Version	XM_006234344.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234344.3

ORF Insert Sequence:

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTCCC TCCTCCCATG 
TCTCCATCCT CCAAGTCGGT GAGCACGCCA AGTGAAGCTG GAAGCCAGGA CTCTGGAGAT 
GGCGCCGTGG GATCTAGGAC GCTGCACTGG GATACAGATC CATCAGCTCT TCAGCTCCAC 
TCAGATTCCG ATTTGGGACG AGGACCTATT AAACTTGGAA TGGCCAAAAT TACTCAAGTC 
GACTTTCCCC CTCGAGAAAT CGTCACATAT ACAAAGGAAA CTCAGACCCC GGTTACAGCT 
CAACCCAAAG AAGATGAGGA GGAGGAAGAT GACGTAGCAG CTCCTAAGCC TCCTGTTGAA 
CCTGAAGAAG AGAAAATTTT AAAAAAAGAC GAAGAGAATG ATAGCAAAGC TCCCCCTCAT 
GAACTAACTG AAGAAGAAAA GCAACAAATT TTACACTCAG AAGAATTTTT AAGTTTCTTT 
GACCATTCTA CAAGAATTGT AGAAAGAGCC CTCTCTGAGC AGATTAACAT CTTCTTTGAC 
TACAGTGGGA GAGATTTGGA AGACAAAGAA GGGGAGATTC AAGCAGGTGC TAAGCTGTCA 
TTAAATCGAC AGTTTTTTGA TGAGCGTTGG TCAAAGCATC GAGTTGTTAG TTGCTTGGAT 
TGGTCATCCC AGTATCCAGA ATTACTTGTG GCTTCCTATA ATAACAATGA AGAGGCTCCT 
CATGAGCCTG ATGGTGTGGC CCTCGTATGG AATATGAAGT ACAAGAAAAC TACCCCAGAG 
TACGTGTTCC ACTGCCAGTC AGCTGTAATG TCTGCTACAT TTGCAAAATT TCATCCAAAT 
CTTGTTGTTG GAGGTACATA CTCAGGCCAA ATTGTGCTTT GGGATAACCG TAGCAATAAA 
AGAACCCCCG TCCAGAGAAC ACCACTGTCA GCTGCTGCAC ACACACACCC TGTCTACTGT 
GTAAATGTTG TTGGAACACA AAATGCTCAT AATCTGATTA GCATCTCTAC TGATGGAAAA 
ATTTGCTCCT GGAGCTTAGA CATGCTTTCC CATCCACAGG ATAGCATGGA GTTGGTTCAT 
AAACAGTCAA AGGCAGTAGC TGTAACATCT ATGTCTTTCC CTGTTGGAGA CGTCAACAAC 
TTTGTTGTGG GCAGTGAAGA AGGTTCTGTG TATACAGCAT GCCGCCATGG CAGCAAAGCC 
GGAATCAGTG AGATGTTTGA GGGACACCAG GGACCCATCA CGGGAATCCA CTGTCACGCA 
GCTGTTGGCG CAGTAGACTT CTCACATCTG TTCGTCACTT CGTCTTTTGA CTGGACAGTA 
AAACTTTGGA CAACTAAGAA TAACAAGCCT TTGTACTCAT TTGAAGATAA TTCAGACTAT 
GTCTATGATG TTATGTGGTC ACCCACCCAC CCAGCCCTGT TTGCCTGTGT GGATGGCATG 
GGGAGACTGG ATCTATGGAA TCTCAACAAT GACACAGAGG TGCCGACTGC CAGCATTTCT 
GTGGAGGGCA ACCCTGCCCT TAACCGTGTG AGATGGACCC ACTCGGGAAG AGAGATCGCC 
GTGGGTGATT CTGAAGGACA GATTGTCATA TATGATGTGG GCGAGCAGAT CGCTGTTCCC 
CGAAATGATG AATGGGCAAG ATTTGGCCGA ACACTCGCAG AGATTAACGC AAGCCGAGCT 
GATGCAGAGG AGGAAGCGGC TACCCGGATT CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dync1i2 ( NM_001270625.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257554.1 Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 3, mRNA.

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CloneID	ORa09421
Clone ID Related Accession (Same CDS sequence)	XM_006234346.3 , NM_001270625.1
Accession Version	NM_001270625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic dynein 1 intermediate chain 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB733957.1, FM110655.1, AC107446.5, BC078764.1 and FM076575.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: U39046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTCCC TCCTCCCATG 
TCTCCATCCT CCAAGTCGGT GAGCACGCCA AGTGAAGCTG GAAGCCAGGA CTCTGGAGAT 
GGCGCCGTGG GATCTAGACG AGGACCTATT AAACTTGGAA TGGCCAAAAT TACTCAAGTC 
GACTTTCCCC CTCGAGAAAT CGTCACATAT ACAAAGGAAA CTCAGACCCC GGTTACAGCT 
CAACCCAAAG AAGATGAGGA GGAGGAAGAT GACGTAGCAG CTCCTAAGCC TCCTGTTGAA 
CCTGAAGAAG AGAAAATTTT AAAAAAAGAC GAAGAGAATG ATAGCAAAGC TCCCCCTCAT 
GAACTAACTG AAGAAGAAAA GCAACAAATT TTACACTCAG AAGAATTTTT AAGTTTCTTT 
GACCATTCTA CAAGAATTGT AGAAAGAGCC CTCTCTGAGC AGATTAACAT CTTCTTTGAC 
TACAGTGGGA GAGATTTGGA AGACAAAGAA GGGGAGATTC AAGCAGGTGC TAAGCTGTCA 
TTAAATCGAC AGTTTTTTGA TGAGCGTTGG TCAAAGCATC GAGTTGTTAG TTGCTTGGAT 
TGGTCATCCC AGTATCCAGA ATTACTTGTG GCTTCCTATA ATAACAATGA AGAGGCTCCT 
CATGAGCCTG ATGGTGTGGC CCTCGTATGG AATATGAAGT ACAAGAAAAC TACCCCAGAG 
TACGTGTTCC ACTGCCAGTC AGCTGTAATG TCTGCTACAT TTGCAAAATT TCATCCAAAT 
CTTGTTGTTG GAGGTACATA CTCAGGCCAA ATTGTGCTTT GGGATAACCG TAGCAATAAA 
AGAACCCCCG TCCAGAGAAC ACCACTGTCA GCTGCTGCAC ACACACACCC TGTCTACTGT 
GTAAATGTTG TTGGAACACA AAATGCTCAT AATCTGATTA GCATCTCTAC TGATGGAAAA 
ATTTGCTCCT GGAGCTTAGA CATGCTTTCC CATCCACAGG ATAGCATGGA GTTGGTTCAT 
AAACAGTCAA AGGCAGTAGC TGTAACATCT ATGTCTTTCC CTGTTGGAGA CGTCAACAAC 
TTTGTTGTGG GCAGTGAAGA AGGTTCTGTG TATACAGCAT GCCGCCATGG CAGCAAAGCC 
GGAATCAGTG AGATGTTTGA GGGACACCAG GGACCCATCA CGGGAATCCA CTGTCACGCA 
GCTGTTGGCG CAGTAGACTT CTCACATCTG TTCGTCACTT CGTCTTTTGA CTGGACAGTA 
AAACTTTGGA CAACTAAGAA TAACAAGCCT TTGTACTCAT TTGAAGATAA TTCAGACTAT 
GTCTATGATG TTATGTGGTC ACCCACCCAC CCAGCCCTGT TTGCCTGTGT GGATGGCATG 
GGGAGACTGG ATCTATGGAA TCTCAACAAT GACACAGAGG TGCCGACTGC CAGCATTTCT 
GTGGAGGGCA ACCCTGCCCT TAACCGTGTG AGATGGACCC ACTCGGGAAG AGAGATCGCC 
GTGGGTGATT CTGAAGGACA GATTGTCATA TATGATGTGG GCGAGCAGAT CGCTGTTCCC 
CGAAATGATG AATGGGCAAG ATTTGGCCGA ACACTCGCAG AGATTAACGC AAGCCGAGCT 
GATGCAGAGG AGGAAGCGGC TACCCGGATT CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257554.1
CDS	355..2193
Translation	MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAAVSVQEESDLEKKRREAEALLQSMGLTTDSPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRRGPIKLGMAKITQVDFPPREIVTYTKETQTPVTAQPKEDEEEEDDVAAPKPPVEPEEEKILKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEEAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNSDYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINASRADAEEEAATRIPA

Target ORF information:

RefSeq Version	NM_001270625.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270625.1

ORF Insert Sequence:

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTCCC TCCTCCCATG 
TCTCCATCCT CCAAGTCGGT GAGCACGCCA AGTGAAGCTG GAAGCCAGGA CTCTGGAGAT 
GGCGCCGTGG GATCTAGACG AGGACCTATT AAACTTGGAA TGGCCAAAAT TACTCAAGTC 
GACTTTCCCC CTCGAGAAAT CGTCACATAT ACAAAGGAAA CTCAGACCCC GGTTACAGCT 
CAACCCAAAG AAGATGAGGA GGAGGAAGAT GACGTAGCAG CTCCTAAGCC TCCTGTTGAA 
CCTGAAGAAG AGAAAATTTT AAAAAAAGAC GAAGAGAATG ATAGCAAAGC TCCCCCTCAT 
GAACTAACTG AAGAAGAAAA GCAACAAATT TTACACTCAG AAGAATTTTT AAGTTTCTTT 
GACCATTCTA CAAGAATTGT AGAAAGAGCC CTCTCTGAGC AGATTAACAT CTTCTTTGAC 
TACAGTGGGA GAGATTTGGA AGACAAAGAA GGGGAGATTC AAGCAGGTGC TAAGCTGTCA 
TTAAATCGAC AGTTTTTTGA TGAGCGTTGG TCAAAGCATC GAGTTGTTAG TTGCTTGGAT 
TGGTCATCCC AGTATCCAGA ATTACTTGTG GCTTCCTATA ATAACAATGA AGAGGCTCCT 
CATGAGCCTG ATGGTGTGGC CCTCGTATGG AATATGAAGT ACAAGAAAAC TACCCCAGAG 
TACGTGTTCC ACTGCCAGTC AGCTGTAATG TCTGCTACAT TTGCAAAATT TCATCCAAAT 
CTTGTTGTTG GAGGTACATA CTCAGGCCAA ATTGTGCTTT GGGATAACCG TAGCAATAAA 
AGAACCCCCG TCCAGAGAAC ACCACTGTCA GCTGCTGCAC ACACACACCC TGTCTACTGT 
GTAAATGTTG TTGGAACACA AAATGCTCAT AATCTGATTA GCATCTCTAC TGATGGAAAA 
ATTTGCTCCT GGAGCTTAGA CATGCTTTCC CATCCACAGG ATAGCATGGA GTTGGTTCAT 
AAACAGTCAA AGGCAGTAGC TGTAACATCT ATGTCTTTCC CTGTTGGAGA CGTCAACAAC 
TTTGTTGTGG GCAGTGAAGA AGGTTCTGTG TATACAGCAT GCCGCCATGG CAGCAAAGCC 
GGAATCAGTG AGATGTTTGA GGGACACCAG GGACCCATCA CGGGAATCCA CTGTCACGCA 
GCTGTTGGCG CAGTAGACTT CTCACATCTG TTCGTCACTT CGTCTTTTGA CTGGACAGTA 
AAACTTTGGA CAACTAAGAA TAACAAGCCT TTGTACTCAT TTGAAGATAA TTCAGACTAT 
GTCTATGATG TTATGTGGTC ACCCACCCAC CCAGCCCTGT TTGCCTGTGT GGATGGCATG 
GGGAGACTGG ATCTATGGAA TCTCAACAAT GACACAGAGG TGCCGACTGC CAGCATTTCT 
GTGGAGGGCA ACCCTGCCCT TAACCGTGTG AGATGGACCC ACTCGGGAAG AGAGATCGCC 
GTGGGTGATT CTGAAGGACA GATTGTCATA TATGATGTGG GCGAGCAGAT CGCTGTTCCC 
CGAAATGATG AATGGGCAAG ATTTGGCCGA ACACTCGCAG AGATTAACGC AAGCCGAGCT 
GATGCAGAGG AGGAAGCGGC TACCCGGATT CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dync1i2 ( NM_053880.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446332.2 Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 1, mRNA.

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CloneID	ORa10942
Clone ID Related Accession (Same CDS sequence)	XM_017591432.1 , NM_053880.2
Accession Version	NM_053880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic dynein 1 intermediate chain 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB733957.1, FM110655.1, AC107446.5, BC078764.1 and FM076575.1. On Jul 21, 2012 this sequence version replaced NM_053880.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: U39044.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTTTT TTCTGAGCAC 
TGGGTCCCTC CTCCCATGTC TCCATCCTCC AAGTCGGTGA GCACGCCAAG TGAAGCTGGA 
AGCCAGGACT CTGGAGATGG CGCCGTGGGA TCTAGGACGC TGCACTGGGA TACAGATCCA 
TCAGCTCTTC AGCTCCACTC AGATTCCGAT TTGGGACGAG GACCTATTAA ACTTGGAATG 
GCCAAAATTA CTCAAGTCGA CTTTCCCCCT CGAGAAATCG TCACATATAC AAAGGAAACT 
CAGACCCCGG TTACAGCTCA ACCCAAAGAA GATGAGGAGG AGGAAGATGA CGTAGCAGCT 
CCTAAGCCTC CTGTTGAACC TGAAGAAGAG AAAATTTTAA AAAAAGACGA AGAGAATGAT 
AGCAAAGCTC CCCCTCATGA ACTAACTGAA GAAGAAAAGC AACAAATTTT ACACTCAGAA 
GAATTTTTAA GTTTCTTTGA CCATTCTACA AGAATTGTAG AAAGAGCCCT CTCTGAGCAG 
ATTAACATCT TCTTTGACTA CAGTGGGAGA GATTTGGAAG ACAAAGAAGG GGAGATTCAA 
GCAGGTGCTA AGCTGTCATT AAATCGACAG TTTTTTGATG AGCGTTGGTC AAAGCATCGA 
GTTGTTAGTT GCTTGGATTG GTCATCCCAG TATCCAGAAT TACTTGTGGC TTCCTATAAT 
AACAATGAAG AGGCTCCTCA TGAGCCTGAT GGTGTGGCCC TCGTATGGAA TATGAAGTAC 
AAGAAAACTA CCCCAGAGTA CGTGTTCCAC TGCCAGTCAG CTGTAATGTC TGCTACATTT 
GCAAAATTTC ATCCAAATCT TGTTGTTGGA GGTACATACT CAGGCCAAAT TGTGCTTTGG 
GATAACCGTA GCAATAAAAG AACCCCCGTC CAGAGAACAC CACTGTCAGC TGCTGCACAC 
ACACACCCTG TCTACTGTGT AAATGTTGTT GGAACACAAA ATGCTCATAA TCTGATTAGC 
ATCTCTACTG ATGGAAAAAT TTGCTCCTGG AGCTTAGACA TGCTTTCCCA TCCACAGGAT 
AGCATGGAGT TGGTTCATAA ACAGTCAAAG GCAGTAGCTG TAACATCTAT GTCTTTCCCT 
GTTGGAGACG TCAACAACTT TGTTGTGGGC AGTGAAGAAG GTTCTGTGTA TACAGCATGC 
CGCCATGGCA GCAAAGCCGG AATCAGTGAG ATGTTTGAGG GACACCAGGG ACCCATCACG 
GGAATCCACT GTCACGCAGC TGTTGGCGCA GTAGACTTCT CACATCTGTT CGTCACTTCG 
TCTTTTGACT GGACAGTAAA ACTTTGGACA ACTAAGAATA ACAAGCCTTT GTACTCATTT 
GAAGATAATT CAGACTATGT CTATGATGTT ATGTGGTCAC CCACCCACCC AGCCCTGTTT 
GCCTGTGTGG ATGGCATGGG GAGACTGGAT CTATGGAATC TCAACAATGA CACAGAGGTG 
CCGACTGCCA GCATTTCTGT GGAGGGCAAC CCTGCCCTTA ACCGTGTGAG ATGGACCCAC 
TCGGGAAGAG AGATCGCCGT GGGTGATTCT GAAGGACAGA TTGTCATATA TGATGTGGGC 
GAGCAGATCG CTGTTCCCCG AAATGATGAA TGGGCAAGAT TTGGCCGAAC ACTCGCAGAG 
ATTAACGCAA GCCGAGCTGA TGCAGAGGAG GAAGCGGCTA CCCGGATTCC TGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446332.2
CDS	355..2271
Translation	MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAAVSVQEESDLEKKRREAEALLQSMGLTTDSPIVFSEHWVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRTLHWDTDPSALQLHSDSDLGRGPIKLGMAKITQVDFPPREIVTYTKETQTPVTAQPKEDEEEEDDVAAPKPPVEPEEEKILKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEEAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNSDYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINASRADAEEEAATRIPA

Target ORF information:

RefSeq Version	NM_053880.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053880.2

ORF Insert Sequence:

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTTTT TTCTGAGCAC 
TGGGTCCCTC CTCCCATGTC TCCATCCTCC AAGTCGGTGA GCACGCCAAG TGAAGCTGGA 
AGCCAGGACT CTGGAGATGG CGCCGTGGGA TCTAGGACGC TGCACTGGGA TACAGATCCA 
TCAGCTCTTC AGCTCCACTC AGATTCCGAT TTGGGACGAG GACCTATTAA ACTTGGAATG 
GCCAAAATTA CTCAAGTCGA CTTTCCCCCT CGAGAAATCG TCACATATAC AAAGGAAACT 
CAGACCCCGG TTACAGCTCA ACCCAAAGAA GATGAGGAGG AGGAAGATGA CGTAGCAGCT 
CCTAAGCCTC CTGTTGAACC TGAAGAAGAG AAAATTTTAA AAAAAGACGA AGAGAATGAT 
AGCAAAGCTC CCCCTCATGA ACTAACTGAA GAAGAAAAGC AACAAATTTT ACACTCAGAA 
GAATTTTTAA GTTTCTTTGA CCATTCTACA AGAATTGTAG AAAGAGCCCT CTCTGAGCAG 
ATTAACATCT TCTTTGACTA CAGTGGGAGA GATTTGGAAG ACAAAGAAGG GGAGATTCAA 
GCAGGTGCTA AGCTGTCATT AAATCGACAG TTTTTTGATG AGCGTTGGTC AAAGCATCGA 
GTTGTTAGTT GCTTGGATTG GTCATCCCAG TATCCAGAAT TACTTGTGGC TTCCTATAAT 
AACAATGAAG AGGCTCCTCA TGAGCCTGAT GGTGTGGCCC TCGTATGGAA TATGAAGTAC 
AAGAAAACTA CCCCAGAGTA CGTGTTCCAC TGCCAGTCAG CTGTAATGTC TGCTACATTT 
GCAAAATTTC ATCCAAATCT TGTTGTTGGA GGTACATACT CAGGCCAAAT TGTGCTTTGG 
GATAACCGTA GCAATAAAAG AACCCCCGTC CAGAGAACAC CACTGTCAGC TGCTGCACAC 
ACACACCCTG TCTACTGTGT AAATGTTGTT GGAACACAAA ATGCTCATAA TCTGATTAGC 
ATCTCTACTG ATGGAAAAAT TTGCTCCTGG AGCTTAGACA TGCTTTCCCA TCCACAGGAT 
AGCATGGAGT TGGTTCATAA ACAGTCAAAG GCAGTAGCTG TAACATCTAT GTCTTTCCCT 
GTTGGAGACG TCAACAACTT TGTTGTGGGC AGTGAAGAAG GTTCTGTGTA TACAGCATGC 
CGCCATGGCA GCAAAGCCGG AATCAGTGAG ATGTTTGAGG GACACCAGGG ACCCATCACG 
GGAATCCACT GTCACGCAGC TGTTGGCGCA GTAGACTTCT CACATCTGTT CGTCACTTCG 
TCTTTTGACT GGACAGTAAA ACTTTGGACA ACTAAGAATA ACAAGCCTTT GTACTCATTT 
GAAGATAATT CAGACTATGT CTATGATGTT ATGTGGTCAC CCACCCACCC AGCCCTGTTT 
GCCTGTGTGG ATGGCATGGG GAGACTGGAT CTATGGAATC TCAACAATGA CACAGAGGTG 
CCGACTGCCA GCATTTCTGT GGAGGGCAAC CCTGCCCTTA ACCGTGTGAG ATGGACCCAC 
TCGGGAAGAG AGATCGCCGT GGGTGATTCT GAAGGACAGA TTGTCATATA TGATGTGGGC 
GAGCAGATCG CTGTTCCCCG AAATGATGAA TGGGCAAGAT TTGGCCGAAC ACTCGCAGAG 
ATTAACGCAA GCCGAGCTGA TGCAGAGGAG GAAGCGGCTA CCCGGATTCC TGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dync1i2 ( XM_006234346.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234408.1 Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X3, mRNA.

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CloneID	ORa09421
Clone ID Related Accession (Same CDS sequence)	XM_006234346.3 , NM_001270625.1
Accession Version	XM_006234346.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic dynein 1 intermediate chain 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTCCC TCCTCCCATG 
TCTCCATCCT CCAAGTCGGT GAGCACGCCA AGTGAAGCTG GAAGCCAGGA CTCTGGAGAT 
GGCGCCGTGG GATCTAGACG AGGACCTATT AAACTTGGAA TGGCCAAAAT TACTCAAGTC 
GACTTTCCCC CTCGAGAAAT CGTCACATAT ACAAAGGAAA CTCAGACCCC GGTTACAGCT 
CAACCCAAAG AAGATGAGGA GGAGGAAGAT GACGTAGCAG CTCCTAAGCC TCCTGTTGAA 
CCTGAAGAAG AGAAAATTTT AAAAAAAGAC GAAGAGAATG ATAGCAAAGC TCCCCCTCAT 
GAACTAACTG AAGAAGAAAA GCAACAAATT TTACACTCAG AAGAATTTTT AAGTTTCTTT 
GACCATTCTA CAAGAATTGT AGAAAGAGCC CTCTCTGAGC AGATTAACAT CTTCTTTGAC 
TACAGTGGGA GAGATTTGGA AGACAAAGAA GGGGAGATTC AAGCAGGTGC TAAGCTGTCA 
TTAAATCGAC AGTTTTTTGA TGAGCGTTGG TCAAAGCATC GAGTTGTTAG TTGCTTGGAT 
TGGTCATCCC AGTATCCAGA ATTACTTGTG GCTTCCTATA ATAACAATGA AGAGGCTCCT 
CATGAGCCTG ATGGTGTGGC CCTCGTATGG AATATGAAGT ACAAGAAAAC TACCCCAGAG 
TACGTGTTCC ACTGCCAGTC AGCTGTAATG TCTGCTACAT TTGCAAAATT TCATCCAAAT 
CTTGTTGTTG GAGGTACATA CTCAGGCCAA ATTGTGCTTT GGGATAACCG TAGCAATAAA 
AGAACCCCCG TCCAGAGAAC ACCACTGTCA GCTGCTGCAC ACACACACCC TGTCTACTGT 
GTAAATGTTG TTGGAACACA AAATGCTCAT AATCTGATTA GCATCTCTAC TGATGGAAAA 
ATTTGCTCCT GGAGCTTAGA CATGCTTTCC CATCCACAGG ATAGCATGGA GTTGGTTCAT 
AAACAGTCAA AGGCAGTAGC TGTAACATCT ATGTCTTTCC CTGTTGGAGA CGTCAACAAC 
TTTGTTGTGG GCAGTGAAGA AGGTTCTGTG TATACAGCAT GCCGCCATGG CAGCAAAGCC 
GGAATCAGTG AGATGTTTGA GGGACACCAG GGACCCATCA CGGGAATCCA CTGTCACGCA 
GCTGTTGGCG CAGTAGACTT CTCACATCTG TTCGTCACTT CGTCTTTTGA CTGGACAGTA 
AAACTTTGGA CAACTAAGAA TAACAAGCCT TTGTACTCAT TTGAAGATAA TTCAGACTAT 
GTCTATGATG TTATGTGGTC ACCCACCCAC CCAGCCCTGT TTGCCTGTGT GGATGGCATG 
GGGAGACTGG ATCTATGGAA TCTCAACAAT GACACAGAGG TGCCGACTGC CAGCATTTCT 
GTGGAGGGCA ACCCTGCCCT TAACCGTGTG AGATGGACCC ACTCGGGAAG AGAGATCGCC 
GTGGGTGATT CTGAAGGACA GATTGTCATA TATGATGTGG GCGAGCAGAT CGCTGTTCCC 
CGAAATGATG AATGGGCAAG ATTTGGCCGA ACACTCGCAG AGATTAACGC AAGCCGAGCT 
GATGCAGAGG AGGAAGCGGC TACCCGGATT CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234408.1
CDS	142..1980
Translation	MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAAVSVQEESDLEKKRREAEALLQSMGLTTDSPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRRGPIKLGMAKITQVDFPPREIVTYTKETQTPVTAQPKEDEEEEDDVAAPKPPVEPEEEKILKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEEAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNSDYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINASRADAEEEAATRIPA

Target ORF information:

RefSeq Version	XM_006234346.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234346.3

ORF Insert Sequence:

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTCCC TCCTCCCATG 
TCTCCATCCT CCAAGTCGGT GAGCACGCCA AGTGAAGCTG GAAGCCAGGA CTCTGGAGAT 
GGCGCCGTGG GATCTAGACG AGGACCTATT AAACTTGGAA TGGCCAAAAT TACTCAAGTC 
GACTTTCCCC CTCGAGAAAT CGTCACATAT ACAAAGGAAA CTCAGACCCC GGTTACAGCT 
CAACCCAAAG AAGATGAGGA GGAGGAAGAT GACGTAGCAG CTCCTAAGCC TCCTGTTGAA 
CCTGAAGAAG AGAAAATTTT AAAAAAAGAC GAAGAGAATG ATAGCAAAGC TCCCCCTCAT 
GAACTAACTG AAGAAGAAAA GCAACAAATT TTACACTCAG AAGAATTTTT AAGTTTCTTT 
GACCATTCTA CAAGAATTGT AGAAAGAGCC CTCTCTGAGC AGATTAACAT CTTCTTTGAC 
TACAGTGGGA GAGATTTGGA AGACAAAGAA GGGGAGATTC AAGCAGGTGC TAAGCTGTCA 
TTAAATCGAC AGTTTTTTGA TGAGCGTTGG TCAAAGCATC GAGTTGTTAG TTGCTTGGAT 
TGGTCATCCC AGTATCCAGA ATTACTTGTG GCTTCCTATA ATAACAATGA AGAGGCTCCT 
CATGAGCCTG ATGGTGTGGC CCTCGTATGG AATATGAAGT ACAAGAAAAC TACCCCAGAG 
TACGTGTTCC ACTGCCAGTC AGCTGTAATG TCTGCTACAT TTGCAAAATT TCATCCAAAT 
CTTGTTGTTG GAGGTACATA CTCAGGCCAA ATTGTGCTTT GGGATAACCG TAGCAATAAA 
AGAACCCCCG TCCAGAGAAC ACCACTGTCA GCTGCTGCAC ACACACACCC TGTCTACTGT 
GTAAATGTTG TTGGAACACA AAATGCTCAT AATCTGATTA GCATCTCTAC TGATGGAAAA 
ATTTGCTCCT GGAGCTTAGA CATGCTTTCC CATCCACAGG ATAGCATGGA GTTGGTTCAT 
AAACAGTCAA AGGCAGTAGC TGTAACATCT ATGTCTTTCC CTGTTGGAGA CGTCAACAAC 
TTTGTTGTGG GCAGTGAAGA AGGTTCTGTG TATACAGCAT GCCGCCATGG CAGCAAAGCC 
GGAATCAGTG AGATGTTTGA GGGACACCAG GGACCCATCA CGGGAATCCA CTGTCACGCA 
GCTGTTGGCG CAGTAGACTT CTCACATCTG TTCGTCACTT CGTCTTTTGA CTGGACAGTA 
AAACTTTGGA CAACTAAGAA TAACAAGCCT TTGTACTCAT TTGAAGATAA TTCAGACTAT 
GTCTATGATG TTATGTGGTC ACCCACCCAC CCAGCCCTGT TTGCCTGTGT GGATGGCATG 
GGGAGACTGG ATCTATGGAA TCTCAACAAT GACACAGAGG TGCCGACTGC CAGCATTTCT 
GTGGAGGGCA ACCCTGCCCT TAACCGTGTG AGATGGACCC ACTCGGGAAG AGAGATCGCC 
GTGGGTGATT CTGAAGGACA GATTGTCATA TATGATGTGG GCGAGCAGAT CGCTGTTCCC 
CGAAATGATG AATGGGCAAG ATTTGGCCGA ACACTCGCAG AGATTAACGC AAGCCGAGCT 
GATGCAGAGG AGGAAGCGGC TACCCGGATT CCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dync1i2 ( XM_017591432.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446921.1 Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X1, mRNA.

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CloneID	ORa10942
Clone ID Related Accession (Same CDS sequence)	XM_017591432.1 , NM_053880.2
Accession Version	XM_017591432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic dynein 1 intermediate chain 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTTTT TTCTGAGCAC 
TGGGTCCCTC CTCCCATGTC TCCATCCTCC AAGTCGGTGA GCACGCCAAG TGAAGCTGGA 
AGCCAGGACT CTGGAGATGG CGCCGTGGGA TCTAGGACGC TGCACTGGGA TACAGATCCA 
TCAGCTCTTC AGCTCCACTC AGATTCCGAT TTGGGACGAG GACCTATTAA ACTTGGAATG 
GCCAAAATTA CTCAAGTCGA CTTTCCCCCT CGAGAAATCG TCACATATAC AAAGGAAACT 
CAGACCCCGG TTACAGCTCA ACCCAAAGAA GATGAGGAGG AGGAAGATGA CGTAGCAGCT 
CCTAAGCCTC CTGTTGAACC TGAAGAAGAG AAAATTTTAA AAAAAGACGA AGAGAATGAT 
AGCAAAGCTC CCCCTCATGA ACTAACTGAA GAAGAAAAGC AACAAATTTT ACACTCAGAA 
GAATTTTTAA GTTTCTTTGA CCATTCTACA AGAATTGTAG AAAGAGCCCT CTCTGAGCAG 
ATTAACATCT TCTTTGACTA CAGTGGGAGA GATTTGGAAG ACAAAGAAGG GGAGATTCAA 
GCAGGTGCTA AGCTGTCATT AAATCGACAG TTTTTTGATG AGCGTTGGTC AAAGCATCGA 
GTTGTTAGTT GCTTGGATTG GTCATCCCAG TATCCAGAAT TACTTGTGGC TTCCTATAAT 
AACAATGAAG AGGCTCCTCA TGAGCCTGAT GGTGTGGCCC TCGTATGGAA TATGAAGTAC 
AAGAAAACTA CCCCAGAGTA CGTGTTCCAC TGCCAGTCAG CTGTAATGTC TGCTACATTT 
GCAAAATTTC ATCCAAATCT TGTTGTTGGA GGTACATACT CAGGCCAAAT TGTGCTTTGG 
GATAACCGTA GCAATAAAAG AACCCCCGTC CAGAGAACAC CACTGTCAGC TGCTGCACAC 
ACACACCCTG TCTACTGTGT AAATGTTGTT GGAACACAAA ATGCTCATAA TCTGATTAGC 
ATCTCTACTG ATGGAAAAAT TTGCTCCTGG AGCTTAGACA TGCTTTCCCA TCCACAGGAT 
AGCATGGAGT TGGTTCATAA ACAGTCAAAG GCAGTAGCTG TAACATCTAT GTCTTTCCCT 
GTTGGAGACG TCAACAACTT TGTTGTGGGC AGTGAAGAAG GTTCTGTGTA TACAGCATGC 
CGCCATGGCA GCAAAGCCGG AATCAGTGAG ATGTTTGAGG GACACCAGGG ACCCATCACG 
GGAATCCACT GTCACGCAGC TGTTGGCGCA GTAGACTTCT CACATCTGTT CGTCACTTCG 
TCTTTTGACT GGACAGTAAA ACTTTGGACA ACTAAGAATA ACAAGCCTTT GTACTCATTT 
GAAGATAATT CAGACTATGT CTATGATGTT ATGTGGTCAC CCACCCACCC AGCCCTGTTT 
GCCTGTGTGG ATGGCATGGG GAGACTGGAT CTATGGAATC TCAACAATGA CACAGAGGTG 
CCGACTGCCA GCATTTCTGT GGAGGGCAAC CCTGCCCTTA ACCGTGTGAG ATGGACCCAC 
TCGGGAAGAG AGATCGCCGT GGGTGATTCT GAAGGACAGA TTGTCATATA TGATGTGGGC 
GAGCAGATCG CTGTTCCCCG AAATGATGAA TGGGCAAGAT TTGGCCGAAC ACTCGCAGAG 
ATTAACGCAA GCCGAGCTGA TGCAGAGGAG GAAGCGGCTA CCCGGATTCC TGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446921.1
CDS	229..2145
Translation	MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAAVSVQEESDLEKKRREAEALLQSMGLTTDSPIVFSEHWVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRTLHWDTDPSALQLHSDSDLGRGPIKLGMAKITQVDFPPREIVTYTKETQTPVTAQPKEDEEEEDDVAAPKPPVEPEEEKILKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEEAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNSDYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINASRADAEEEAATRIPA

Target ORF information:

RefSeq Version	XM_017591432.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein cytoplasmic 1 intermediate chain 2 (Dync1i2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591432.1

ORF Insert Sequence:

ATGTCAGACA AAAGTGAATT AAAAGCTGAG TTGGAACGTA AGAAGCAACG GTTGGCCCAA 
ATCAGAGAAG AAAAGAAGAG GAAAGAAGAG GAAAGGAAAA AAAAAGAAAC TGATCAGAAG 
AAGGAAGCTG CAGTCTCTGT GCAGGAAGAG TCTGACCTTG AAAAGAAGAG AAGAGAAGCC 
GAAGCCTTGC TTCAGAGCAT GGGGCTAACT ACAGACTCCC CAATTGTTTT TTCTGAGCAC 
TGGGTCCCTC CTCCCATGTC TCCATCCTCC AAGTCGGTGA GCACGCCAAG TGAAGCTGGA 
AGCCAGGACT CTGGAGATGG CGCCGTGGGA TCTAGGACGC TGCACTGGGA TACAGATCCA 
TCAGCTCTTC AGCTCCACTC AGATTCCGAT TTGGGACGAG GACCTATTAA ACTTGGAATG 
GCCAAAATTA CTCAAGTCGA CTTTCCCCCT CGAGAAATCG TCACATATAC AAAGGAAACT 
CAGACCCCGG TTACAGCTCA ACCCAAAGAA GATGAGGAGG AGGAAGATGA CGTAGCAGCT 
CCTAAGCCTC CTGTTGAACC TGAAGAAGAG AAAATTTTAA AAAAAGACGA AGAGAATGAT 
AGCAAAGCTC CCCCTCATGA ACTAACTGAA GAAGAAAAGC AACAAATTTT ACACTCAGAA 
GAATTTTTAA GTTTCTTTGA CCATTCTACA AGAATTGTAG AAAGAGCCCT CTCTGAGCAG 
ATTAACATCT TCTTTGACTA CAGTGGGAGA GATTTGGAAG ACAAAGAAGG GGAGATTCAA 
GCAGGTGCTA AGCTGTCATT AAATCGACAG TTTTTTGATG AGCGTTGGTC AAAGCATCGA 
GTTGTTAGTT GCTTGGATTG GTCATCCCAG TATCCAGAAT TACTTGTGGC TTCCTATAAT 
AACAATGAAG AGGCTCCTCA TGAGCCTGAT GGTGTGGCCC TCGTATGGAA TATGAAGTAC 
AAGAAAACTA CCCCAGAGTA CGTGTTCCAC TGCCAGTCAG CTGTAATGTC TGCTACATTT 
GCAAAATTTC ATCCAAATCT TGTTGTTGGA GGTACATACT CAGGCCAAAT TGTGCTTTGG 
GATAACCGTA GCAATAAAAG AACCCCCGTC CAGAGAACAC CACTGTCAGC TGCTGCACAC 
ACACACCCTG TCTACTGTGT AAATGTTGTT GGAACACAAA ATGCTCATAA TCTGATTAGC 
ATCTCTACTG ATGGAAAAAT TTGCTCCTGG AGCTTAGACA TGCTTTCCCA TCCACAGGAT 
AGCATGGAGT TGGTTCATAA ACAGTCAAAG GCAGTAGCTG TAACATCTAT GTCTTTCCCT 
GTTGGAGACG TCAACAACTT TGTTGTGGGC AGTGAAGAAG GTTCTGTGTA TACAGCATGC 
CGCCATGGCA GCAAAGCCGG AATCAGTGAG ATGTTTGAGG GACACCAGGG ACCCATCACG 
GGAATCCACT GTCACGCAGC TGTTGGCGCA GTAGACTTCT CACATCTGTT CGTCACTTCG 
TCTTTTGACT GGACAGTAAA ACTTTGGACA ACTAAGAATA ACAAGCCTTT GTACTCATTT 
GAAGATAATT CAGACTATGT CTATGATGTT ATGTGGTCAC CCACCCACCC AGCCCTGTTT 
GCCTGTGTGG ATGGCATGGG GAGACTGGAT CTATGGAATC TCAACAATGA CACAGAGGTG 
CCGACTGCCA GCATTTCTGT GGAGGGCAAC CCTGCCCTTA ACCGTGTGAG ATGGACCCAC 
TCGGGAAGAG AGATCGCCGT GGGTGATTCT GAAGGACAGA TTGTCATATA TGATGTGGGC 
GAGCAGATCG CTGTTCCCCG AAATGATGAA TGGGCAAGAT TTGGCCGAAC ACTCGCAGAG 
ATTAACGCAA GCCGAGCTGA TGCAGAGGAG GAAGCGGCTA CCCGGATTCC TGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84.
Journal of neuroscience research92(9)1143-54(2014 Sep)
Blasier KR,Humsi MK,Ha J,Ross MW,Smiley WR,Inamdar NA,Mitchell DJ,Lo KW,Pfister KK

Integrated proteomics identified novel activation of dynein IC2-GR-COX-1 signaling in neurofibromatosis type I (NF1) disease model cells.
Molecular & cellular proteomics : MCP12(5)1377-94(2013 May)
Hirayama M,Kobayashi D,Mizuguchi S,Morikawa T,Nagayama M,Midorikawa U,Wilson MM,Nambu AN,Yoshizawa AC,Kawano S,Araki N

Cytoplasmic dynein.
Biochemical Society transactions39(5)1169-78(2011 Oct)
Allan VJ

PEX14 is required for microtubule-based peroxisome motility in human cells.
Journal of cell science124(Pt 10)1759-68(2011 May)
Bharti P,Schliebs W,Schievelbusch T,Neuhaus A,David C,Kock K,Herrmann C,Meyer HE,Wiese S,Warscheid B,Theiss C,Erdmann R

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
The EMBO journal30(8)1520-35(2011 Apr)
Jakobsen L,Vanselow K,Skogs M,Toyoda Y,Lundberg E,Poser I,Falkenby LG,Bennetzen M,Westendorf J,Nigg EA,Uhlen M,Hyman AA,Andersen JS

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Dync1i2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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