SACM1L cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	SACM1L
Entrez Gene ID	116482
Full Name	SAC1 like phosphatidylinositide phosphatase
Synonyms	Sac1
General protein information	Preferred Names SAC1 like phosphatidylinositide phosphatase Names phosphatidylinositide phosphatase SAC1 SAC1 suppressor of actin mutations 1-like suppressor of actin mutations 1-like protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001357497.1	NP_001344426.1	phosphatidylinositide phosphatase SAC1 isoform 1x
NM_053798.3	NP_446250.1	phosphatidylinositide phosphatase SAC1 isoform 1
NM_053798.2	NP_446250.1	phosphatidylinositide phosphatase SAC1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SACM1L	NP_001095601.1
Mus musculus (house mouse)	Sacm1l	NP_109617.1
Drosophila melanogaster (fruit fly)	Sac1	NP_001189016.1
Magnaporthe oryzae (rice blast fungus)	MGG_11279	XP_003714069.1
Arabidopsis thaliana (thale cress)	RHD4	NP_190714.2
Pan troglodytes (chimpanzee)	SACM1L	XP_516409.4
Caenorhabditis elegans (roundworm)	sac-1	NP_492518.1
Eremothecium gossypii	AGOS_AER447C	NP_985302.1
Gallus gallus (chicken)	SACM1L	XP_418799.4
Canis lupus familiaris (dog)	SACM1L	XP_541911.2
Danio rerio (zebrafish)	sacm1la	NP_001038343.1
Danio rerio (zebrafish)	sacm1lb	NP_001074093.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000891	XP_316868.5
Schizosaccharomyces pombe (fission yeast)	SPBC19F5.03	NP_596541.1
Arabidopsis thaliana (thale cress)	ATSAC1B	NP_201403.2
Homo sapiens (human)	SACM1L	NP_054735.3
	SACM1L	XP_001114514.1
Rattus norvegicus (Norway rat)	Sacm1l	NP_446250.1
Saccharomyces cerevisiae (baker's yeast)	SAC1	NP_012710.1
Kluyveromyces lactis	KLLA0B14388g	XP_452171.1
Neurospora crassa	NCU00896	XP_964812.1
Oryza sativa (rice)	Os02g0554300	NP_001047117.1
Oryza sativa (rice)	Os11g0309000	NP_001067755.1

Gene Ontology
Bibliography

Related articles in PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Loughran G, Chou MY, Ivanov IP, Jungreis I, Kellis M, Kiran AM, Baranov PV, Atkins JF
Nucleic acids research42(14)8928-38(2014 Aug)

A four-step cycle driven by PI(4)P hydrolysis directs sterol/PI(4)P exchange by the ER-Golgi tether OSBP.
Mesmin B, Bigay J, Moser von Filseck J, Lacas-Gervais S, Drin G, Antonny B
Cell155(4)830-43(2013 Nov)

Root hair defective4 encodes a phosphatidylinositol-4-phosphate phosphatase required for proper root hair development in Arabidopsis thaliana.
Thole JM, Vermeer JE, Zhang Y, Gadella TW Jr, Nielsen E
The Plant cell20(2)381-95(2008 Feb)

The SAC domain-containing protein gene family in Arabidopsis.
Zhong R, Ye ZH
Plant physiology132(2)544-55(2003 Jun)

Functional characterization of a mammalian Sac1 and mutants exhibiting substrate-specific defects in phosphoinositide phosphatase activity.
Nemoto Y, Kearns BG, Wenk MR, Chen H, Mori K, Alb JG Jr, De Camilli P, Bankaitis VA
The Journal of biological chemistry275(44)34293-305(2000 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SACM1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SACM1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10675	NM_053798.2 Latest version!	Rattus norvegicus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa63272	NM_053798.3 Latest version!	Rattus norvegicus SAC1 like phosphatidylinositide phosphatase (Sacm1l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa63273	NM_001357497.1 Latest version!	Rattus norvegicus SAC1 like phosphatidylinositide phosphatase (Sacm1l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SACM1L ( NM_053798.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446250.1 Rattus norvegicus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), mRNA.

Price & Availability↑

CloneID	ORa10675
Clone ID Related Accession (Same CDS sequence)	NM_053798.2
Accession Version	NM_053798.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-31
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositide phosphatase SAC1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF251186.1. On Apr 4, 2007 this sequence version replaced NM_053798.1. Sequence Note: The sequence AF251186.1 is from a chimeric clone. Only the SAC1 (supressor of actin mutations 1, homolog)-like sequence region was propagated into this RefSeq record. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCA CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAAGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGCGTGTC TACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCT TCTGCTGTTA CAAGACCAAT ATATGGGATA 
ATGGGCACAA TCCATCTGGT GGCAGGCAAT TATCTTGTTG TCATTACCAA AAAGATGAAA 
GTAGGTGAAT TTTTTAATCA TGTAATCTGG AAAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTACACTT AACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TACGGATGGA TTTTACTTTT CAACAACATA TGACTTGACC 
CATACTTTGC AACGGCTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCCTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GAGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCTGTGTTG CATGGCTTTA TTACTATGCA TTCATGTTCT 
ATTAACGGGA AATATTTTGA TTGGATTCTC ATCTCCCGGA GGAGTTGTTT CAGAGCCGGT 
GTGCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAACAGATTG TGCACTACAG TGGGAACAGG GCTTCATTTG TACAGACTCG AGGATCAATA 
CCTGTTTTCT GGTCACAAAG ACCAAATCTA AAGTACAAAC CAGATCCACA GATCAACAAA 
GTAGCAAATC ACATGGATGG TTTCCAAAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAACAAGTAA TAATTAATCT GGTGAACCAT AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTTGCAAAAA TGGTGTCATC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGACTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CTGGCAAGGT GGTGACAAAC 
CAGGAAGGAG TTTTCCGCAG CAACTGTATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGGCGTTC CCTTCAGGCA CAGCTGCAGA GACTAGGAGT TTTGCATGTG 
GGACAAAAGC TTGAAGAACA AGATGAGTTT GAGAAGATTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCA GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTG GTAATGGATG GCTTCAACTC ATTATTACGG 
TACTACAAGA ACAACTTTTC TGATGGATTT AGACAAGATT CTATAGACTT ATTTCTTGGA 
AACTACTCAG TGGATGAATT AGACTCTCAC AGTCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTT 
GTACAGAAAG AAAAGATAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446250.1
CDS	42..1805
Translation	MAATAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAVKKDVPPSAVTRPIYGIMGTIHLVAGNYLVVITKKMKVGEFFNHVIWKATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLSTDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPVFWSQRPNLKYKPDPQINKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFAKMVSSLGSGMIRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGKVVTNQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLVMDGFNSLLRYYKNNFSDGFRQDSIDLFLGNYSVDELDSHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID

Target ORF information:

RefSeq Version	NM_053798.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053798.2

ORF Insert Sequence:

ATGGCGGCCA CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAAGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGCGTGTC TACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCT TCTGCTGTTA CAAGACCAAT ATATGGGATA 
ATGGGCACAA TCCATCTGGT GGCAGGCAAT TATCTTGTTG TCATTACCAA AAAGATGAAA 
GTAGGTGAAT TTTTTAATCA TGTAATCTGG AAAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTACACTT AACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TACGGATGGA TTTTACTTTT CAACAACATA TGACTTGACC 
CATACTTTGC AACGGCTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCCTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GAGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCTGTGTTG CATGGCTTTA TTACTATGCA TTCATGTTCT 
ATTAACGGGA AATATTTTGA TTGGATTCTC ATCTCCCGGA GGAGTTGTTT CAGAGCCGGT 
GTGCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAACAGATTG TGCACTACAG TGGGAACAGG GCTTCATTTG TACAGACTCG AGGATCAATA 
CCTGTTTTCT GGTCACAAAG ACCAAATCTA AAGTACAAAC CAGATCCACA GATCAACAAA 
GTAGCAAATC ACATGGATGG TTTCCAAAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAACAAGTAA TAATTAATCT GGTGAACCAT AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTTGCAAAAA TGGTGTCATC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGACTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CTGGCAAGGT GGTGACAAAC 
CAGGAAGGAG TTTTCCGCAG CAACTGTATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGGCGTTC CCTTCAGGCA CAGCTGCAGA GACTAGGAGT TTTGCATGTG 
GGACAAAAGC TTGAAGAACA AGATGAGTTT GAGAAGATTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCA GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTG GTAATGGATG GCTTCAACTC ATTATTACGG 
TACTACAAGA ACAACTTTTC TGATGGATTT AGACAAGATT CTATAGACTT ATTTCTTGGA 
AACTACTCAG TGGATGAATT AGACTCTCAC AGTCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTT 
GTACAGAAAG AAAAGATAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SACM1L ( NM_053798.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446250.1 Rattus norvegicus SAC1 like phosphatidylinositide phosphatase (Sacm1l), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa63272
Clone ID Related Accession (Same CDS sequence)	NM_053798.3
Accession Version	NM_053798.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositide phosphatase SAC1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07071818.1, FM060902.1, FM108115.1, FM108005.1, FM134775.1 and FM095674.1. On Oct 27, 2017 this sequence version replaced NM_053798.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1). COMPLETENESS: complete on the 3' end.

ATGGCGGCCA CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAAGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGCGTGTC TACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCT TCTGCTGTTA CAAGACCAAT ATATGGGATA 
ATGGGCACAA TCCATCTGGT GGCAGGCAAT TATCTTGTTG TCATTACCAA AAAGATGAAA 
GTAGGTGAAT TTTTTAATCA TGTAATCTGG AAAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTACACTT AACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TACGGATGGA TTTTACTTTT CAACAACATA TGACTTGACC 
CATACTTTGC AACGGCTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCCTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GAGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCTGTGTTG CATGGCTTTA TTACTATGCA TTCATGTTCT 
ATTAACGGGA AATATTTTGA TTGGATTCTC ATCTCCCGGA GGAGTTGTTT CAGAGCCGGT 
GTGCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAACAGATTG TGCACTACAG TGGGAACAGG GCTTCATTTG TACAGACTCG AGGATCAATA 
CCTGTTTTCT GGTCACAAAG ACCAAATCTA AAGTACAAAC CAGATCCACA GATCAACAAA 
GTAGCAAATC ACATGGATGG TTTCCAAAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAACAAGTAA TAATTAATCT GGTGAACCAT AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTTGCAAAAA TGGTGTCATC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGACTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CTGGCAAGGT GGTGACAAAC 
CAGGAAGGAG TTTTCCGCAG CAACTGTATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGGCGTTC CCTTCAGGCA CAGCTGCAGA GACTAGGAGT TTTGCATGTG 
GGACAAAAGC TTGAAGAACA AGATGAGTTT GAGAAGATTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCA GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTG GTAATGGATG GCTTCAACTC ATTATTACGG 
TACTACAAAA ACAACTTTTC TGATGGATTT AGACAAGATT CTATAGACTT ATTTCTTGGA 
AACTACTCAG TGGATGAATT AGACTCTCAC AGTCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTT 
GTACAGAAAG AAAAGATAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446250.1
CDS	93..1856
Translation	MAATAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAVKKDVPPSAVTRPIYGIMGTIHLVAGNYLVVITKKMKVGEFFNHVIWKATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLSTDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPVFWSQRPNLKYKPDPQINKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFAKMVSSLGSGMIRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGKVVTNQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLVMDGFNSLLRYYKNNFSDGFRQDSIDLFLGNYSVDELDSHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID

Target ORF information:

RefSeq Version	NM_053798.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SAC1 like phosphatidylinositide phosphatase (Sacm1l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053798.3

ORF Insert Sequence:

ATGGCGGCCA CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAAGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGCGTGTC TACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCT TCTGCTGTTA CAAGACCAAT ATATGGGATA 
ATGGGCACAA TCCATCTGGT GGCAGGCAAT TATCTTGTTG TCATTACCAA AAAGATGAAA 
GTAGGTGAAT TTTTTAATCA TGTAATCTGG AAAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTACACTT AACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TACGGATGGA TTTTACTTTT CAACAACATA TGACTTGACC 
CATACTTTGC AACGGCTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCCTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GAGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCTGTGTTG CATGGCTTTA TTACTATGCA TTCATGTTCT 
ATTAACGGGA AATATTTTGA TTGGATTCTC ATCTCCCGGA GGAGTTGTTT CAGAGCCGGT 
GTGCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAACAGATTG TGCACTACAG TGGGAACAGG GCTTCATTTG TACAGACTCG AGGATCAATA 
CCTGTTTTCT GGTCACAAAG ACCAAATCTA AAGTACAAAC CAGATCCACA GATCAACAAA 
GTAGCAAATC ACATGGATGG TTTCCAAAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAACAAGTAA TAATTAATCT GGTGAACCAT AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTTGCAAAAA TGGTGTCATC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGACTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CTGGCAAGGT GGTGACAAAC 
CAGGAAGGAG TTTTCCGCAG CAACTGTATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGGCGTTC CCTTCAGGCA CAGCTGCAGA GACTAGGAGT TTTGCATGTG 
GGACAAAAGC TTGAAGAACA AGATGAGTTT GAGAAGATTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCA GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTG GTAATGGATG GCTTCAACTC ATTATTACGG 
TACTACAAAA ACAACTTTTC TGATGGATTT AGACAAGATT CTATAGACTT ATTTCTTGGA 
AACTACTCAG TGGATGAATT AGACTCTCAC AGTCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTT 
GTACAGAAAG AAAAGATAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SACM1L ( NM_001357497.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001344426.1 Rattus norvegicus SAC1 like phosphatidylinositide phosphatase (Sacm1l), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa63273
Clone ID Related Accession (Same CDS sequence)	NM_001357497.1
Accession Version	NM_001357497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositide phosphatase SAC1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07071818.1, FM060902.1, FM108115.1, FM108005.1, FM134775.1 and FM095674.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCA CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAAGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGCGTGTC TACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCT TCTGCTGTTA CAAGACCAAT ATATGGGATA 
ATGGGCACAA TCCATCTGGT GGCAGGCAAT TATCTTGTTG TCATTACCAA AAAGATGAAA 
GTAGGTGAAT TTTTTAATCA TGTAATCTGG AAAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTACACTT AACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TACGGATGGA TTTTACTTTT CAACAACATA TGACTTGACC 
CATACTTTGC AACGGCTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCCTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GAGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCTGTGTTG CATGGCTTTA TTACTATGCA TTCATGTTCT 
ATTAACGGGA AATATTTTGA TTGGATTCTC ATCTCCCGGA GGAGTTGTTT CAGAGCCGGT 
GTGCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAACAGATTG TGCACTACAG TGGGAACAGG GCTTCATTTG TACAGACTCG AGGATCAATA 
CCTGTTTTCT GGTCACAAAG ACCAAATCTA AAGTACAAAC CAGATCCACA GATCAACAAA 
GTAGCAAATC ACATGGATGG TTTCCAAAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAACAAGTAA TAATTAATCT GGTGAACCAT AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTTGCAAAAA TGGTGTCATC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGACTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CTGGCAAGGT GGTGACAAAC 
CAGGAAGGAG TTTTCCGCAG CAACTGTATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGGCGTTC CCTTCAGGCA CAGCTGCAGA GACTAGGAGT TTTGCATGTG 
GGACAAAAGC TTGAAGAACA AGATGAGTTT GAGAAGATTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCA GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTG GTAATGGATG GCTTCAACTC ATTATTACGG 
TACTACAAAA ACAACTTTTC TGATGGATTT AGACAAGATT CTATAGACTT ATTTCTTGGA 
AACTACTCAG TGGATGAATT AGACTCTCAC AGTCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTT 
GTACAGAAAG AAAAGATAGA CTGAATTTGT GTTTGTGGAA AGCGGCTTGG CTTGGAAGAC 
TCCATGATGC AGAACTGGAG TCTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344426.1
CDS	93..1922
Translation	MAATAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAVKKDVPPSAVTRPIYGIMGTIHLVAGNYLVVITKKMKVGEFFNHVIWKATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLSTDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPVFWSQRPNLKYKPDPQINKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFAKMVSSLGSGMIRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGKVVTNQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLVMDGFNSLLRYYKNNFSDGFRQDSIDLFLGNYSVDELDSHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID*ICVCGKRLGLEDSMMQNWSLY

Target ORF information:

RefSeq Version	NM_001357497.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SAC1 like phosphatidylinositide phosphatase (Sacm1l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357497.1

ORF Insert Sequence:

ATGGCGGCCA CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAAGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGCGTGTC TACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCT TCTGCTGTTA CAAGACCAAT ATATGGGATA 
ATGGGCACAA TCCATCTGGT GGCAGGCAAT TATCTTGTTG TCATTACCAA AAAGATGAAA 
GTAGGTGAAT TTTTTAATCA TGTAATCTGG AAAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTACACTT AACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TACGGATGGA TTTTACTTTT CAACAACATA TGACTTGACC 
CATACTTTGC AACGGCTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCCTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GAGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCTGTGTTG CATGGCTTTA TTACTATGCA TTCATGTTCT 
ATTAACGGGA AATATTTTGA TTGGATTCTC ATCTCCCGGA GGAGTTGTTT CAGAGCCGGT 
GTGCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAACAGATTG TGCACTACAG TGGGAACAGG GCTTCATTTG TACAGACTCG AGGATCAATA 
CCTGTTTTCT GGTCACAAAG ACCAAATCTA AAGTACAAAC CAGATCCACA GATCAACAAA 
GTAGCAAATC ACATGGATGG TTTCCAAAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAACAAGTAA TAATTAATCT GGTGAACCAT AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTTGCAAAAA TGGTGTCATC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGACTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CTGGCAAGGT GGTGACAAAC 
CAGGAAGGAG TTTTCCGCAG CAACTGTATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGGCGTTC CCTTCAGGCA CAGCTGCAGA GACTAGGAGT TTTGCATGTG 
GGACAAAAGC TTGAAGAACA AGATGAGTTT GAGAAGATTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCA GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTG GTAATGGATG GCTTCAACTC ATTATTACGG 
TACTACAAAA ACAACTTTTC TGATGGATTT AGACAAGATT CTATAGACTT ATTTCTTGGA 
AACTACTCAG TGGATGAATT AGACTCTCAC AGTCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTT 
GTACAGAAAG AAAAGATAGA CTGAATTTGT GTTTGTGGAA AGCGGCTTGG CTTGGAAGAC 
TCCATGATGC AGAACTGGAG TCTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Nucleic acids research42(14)8928-38(2014 Aug)
Loughran G,Chou MY,Ivanov IP,Jungreis I,Kellis M,Kiran AM,Baranov PV,Atkins JF

A four-step cycle driven by PI(4)P hydrolysis directs sterol/PI(4)P exchange by the ER-Golgi tether OSBP.
Cell155(4)830-43(2013 Nov)
Mesmin B,Bigay J,Moser von Filseck J,Lacas-Gervais S,Drin G,Antonny B

Root hair defective4 encodes a phosphatidylinositol-4-phosphate phosphatase required for proper root hair development in Arabidopsis thaliana.
The Plant cell20(2)381-95(2008 Feb)
Thole JM,Vermeer JE,Zhang Y,Gadella TW Jr,Nielsen E

The SAC domain-containing protein gene family in Arabidopsis.
Plant physiology132(2)544-55(2003 Jun)
Zhong R,Ye ZH

Functional characterization of a mammalian Sac1 and mutants exhibiting substrate-specific defects in phosphoinositide phosphatase activity.
The Journal of biological chemistry275(44)34293-305(2000 Nov)
Nemoto Y,Kearns BG,Wenk MR,Chen H,Mori K,Alb JG Jr,De Camilli P,Bankaitis VA

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

SACM1L cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報