Sacm1l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sacm1l
Entrez Gene ID	83493
Full Name	SAC1 suppressor of actin mutations 1-like (yeast)
Synonyms	SAC1,Sac1p,mKIAA0851
General protein information	Preferred Names SAC1 suppressor of actin mutations 1-like (yeast) Names phosphatidylinositide phosphatase SAC1 polyphosphatidylinositol phosphatase suppressor of actin mutations 1-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F4\|9 74.08 cM
Summary	This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_030692.5	NP_109617.1	phosphatidylinositide phosphatase SAC1 isoform 1
NM_030692.4	NP_109617.1	phosphatidylinositide phosphatase SAC1
XM_017313703.1	XP_017169192.1	phosphatidylinositide phosphatase SAC1 isoform X1
NM_001357485.1	NP_001344414.1	phosphatidylinositide phosphatase SAC1 isoform 1x

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SACM1L	NP_001095601.1
Mus musculus (house mouse)	Sacm1l	NP_109617.1
Drosophila melanogaster (fruit fly)	Sac1	NP_001189016.1
Magnaporthe oryzae (rice blast fungus)	MGG_11279	XP_003714069.1
Arabidopsis thaliana (thale cress)	RHD4	NP_190714.2
Pan troglodytes (chimpanzee)	SACM1L	XP_516409.4
Caenorhabditis elegans (roundworm)	sac-1	NP_492518.1
Eremothecium gossypii	AGOS_AER447C	NP_985302.1
Gallus gallus (chicken)	SACM1L	XP_418799.4
Canis lupus familiaris (dog)	SACM1L	XP_541911.2
Danio rerio (zebrafish)	sacm1la	NP_001038343.1
Danio rerio (zebrafish)	sacm1lb	NP_001074093.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000891	XP_316868.5
Schizosaccharomyces pombe (fission yeast)	SPBC19F5.03	NP_596541.1
Arabidopsis thaliana (thale cress)	ATSAC1B	NP_201403.2
Homo sapiens (human)	SACM1L	NP_054735.3
	SACM1L	XP_001114514.1
Rattus norvegicus (Norway rat)	Sacm1l	NP_446250.1
Saccharomyces cerevisiae (baker's yeast)	SAC1	NP_012710.1
Kluyveromyces lactis	KLLA0B14388g	XP_452171.1
Neurospora crassa	NCU00896	XP_964812.1
Oryza sativa (rice)	Os02g0554300	NP_001047117.1
Oryza sativa (rice)	Os11g0309000	NP_001067755.1

Gene Ontology
Bibliography

Related articles in PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Loughran G, Chou MY, Ivanov IP, Jungreis I, Kellis M, Kiran AM, Baranov PV, Atkins JF
Nucleic acids research42(14)8928-38(2014 Aug)

A novel probe for phosphatidylinositol 4-phosphate reveals multiple pools beyond the Golgi.
Hammond GR, Machner MP, Balla T
The Journal of cell biology205(1)113-26(2014 Apr)

High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes.
Schwenk J, Harmel N, Brechet A, Zolles G, Berkefeld H, M?ller CS, Bildl W, Baehrens D, H?ber B, Kulik A, Kl?cker N, Schulte U, Fakler B
Neuron74(4)621-33(2012 May)

Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease.
Tkatchenko TV, Moreno-Rodriguez RA, Conway SJ, Molkentin JD, Markwald RR, Tkatchenko AV
Physiological genomics39(3)160-8(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sac1 executes an essential household function in mammals that involves organization of both Golgi membranes and mitotic spindles
Title: The Sac1 phosphoinositide phosphatase regulates Golgi membrane morphology and mitotic spindle organization in mammals.

These results suggest direct roles for p38 MAPK and SAC1 in transmitting growth signals to the secretory machinery.
Title: Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1.

The following Sacm1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sacm1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83651	NM_001357485.1 Latest version!	Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OMu08368	NM_030692.5 Latest version!	Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu83007	XM_017313703.1 Latest version!	Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu08368	NM_030692.4	Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sacm1l ( NM_001357485.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344414.1 Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu83651
Clone ID Related Accession (Same CDS sequence)	NM_001357485.1
Accession Version	NM_001357485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	phosphatidylinositide phosphatase SAC1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY127420.1, CJ173186.1, AK161111.1, CN539161.1 and CO044347.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. ##Evidence-Data-START## Transcript exon combination :: AK154759.1, SRR1660817.136215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAGGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGAGTGTC CACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCC TCTGCTGTTA CAAGACCAAT ATTTGGGATA 
CTGGGCACAA TCCATCTGGT GGCAGGTAAT TATCTTGTTG TCATCACCAA AAAGATGAAA 
GTAGGTGAAT GTTTTAATCA TGCGGTCTGG AGAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTGCACTT GACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TATGGATGGA TTTTACTTTT CAACAACATA TGACTTGACT 
CATACTTTGC AGCGACTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCTTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GGGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCAGTGTTG CATGGCTTTA TTACTATGCA CTCATGTTCT 
ATTAATGGAA AATATTTTGA TTGGATTCTC ATCTCCCGAA GGAGTTGTTT CAGAGCTGGT 
GTTCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAGCAGATTG TGCACTACAG CGGGAACAGG GCTTCGTTTG TCCAGACTCG AGGATCAATA 
CCTATTTTCT GGTCACAGAG ACCAAATCTA AAGTACAAAC CACATCCACA GATCAGCAAA 
GTAGCAAATC ACATGGATGG TTTCCAGAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAGCAAGTAA TAATTAATCT GGTGAACCAC AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTCGCAAACA TGGTGTCCTC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGATTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CCGGCAAGGT GGTGACAAAC 
CAGGACGGAG TTTTCCGCAG CAACTGCATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGACGTTC CCTTCAGGCA CAGCTGCAGA GGCTAGGAGT TTTGCATGTG 
GGACAGAAGC TTGAAGAGCA AGATGAATTT GAGAAGACTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCG GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTA TTAATGGATG GCTTCAACTC ATTATTACGG 
TATTACAAGA ACAACTTTTC TGATGGATTT AGACAAGATT CCATAGACTT ATTTCTTGGA 
AATTATTCAG TGGATGAATT AGAATCTCAT AGCCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTG 
GTCCAGAAAG AAAAGATAGA CTGAATTTGT GTTTGTGGAA AGCGGCTTGG CTTGGAAGAC 
TCCATGATGC AGAACTGGAG TCTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344414.1
CDS	138..1967
Translation	MAAAAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAVKKDVPPSAVTRPIFGILGTIHLVAGNYLVVITKKMKVGECFNHAVWRATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLSMDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPIFWSQRPNLKYKPHPQISKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFANMVSSLGSGMIRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGKVVTNQDGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKTYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLLMDGFNSLLRYYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID*ICVCGKRLGLEDSMMQNWSLY

Target ORF information:

RefSeq Version	NM_001357485.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357485.1

ORF Insert Sequence:

ATGGCGGCCG CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAGGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGAGTGTC CACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCC TCTGCTGTTA CAAGACCAAT ATTTGGGATA 
CTGGGCACAA TCCATCTGGT GGCAGGTAAT TATCTTGTTG TCATCACCAA AAAGATGAAA 
GTAGGTGAAT GTTTTAATCA TGCGGTCTGG AGAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTGCACTT GACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TATGGATGGA TTTTACTTTT CAACAACATA TGACTTGACT 
CATACTTTGC AGCGACTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCTTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GGGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCAGTGTTG CATGGCTTTA TTACTATGCA CTCATGTTCT 
ATTAATGGAA AATATTTTGA TTGGATTCTC ATCTCCCGAA GGAGTTGTTT CAGAGCTGGT 
GTTCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAGCAGATTG TGCACTACAG CGGGAACAGG GCTTCGTTTG TCCAGACTCG AGGATCAATA 
CCTATTTTCT GGTCACAGAG ACCAAATCTA AAGTACAAAC CACATCCACA GATCAGCAAA 
GTAGCAAATC ACATGGATGG TTTCCAGAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAGCAAGTAA TAATTAATCT GGTGAACCAC AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTCGCAAACA TGGTGTCCTC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGATTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CCGGCAAGGT GGTGACAAAC 
CAGGACGGAG TTTTCCGCAG CAACTGCATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGACGTTC CCTTCAGGCA CAGCTGCAGA GGCTAGGAGT TTTGCATGTG 
GGACAGAAGC TTGAAGAGCA AGATGAATTT GAGAAGACTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCG GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTA TTAATGGATG GCTTCAACTC ATTATTACGG 
TATTACAAGA ACAACTTTTC TGATGGATTT AGACAAGATT CCATAGACTT ATTTCTTGGA 
AATTATTCAG TGGATGAATT AGAATCTCAT AGCCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTG 
GTCCAGAAAG AAAAGATAGA CTGAATTTGT GTTTGTGGAA AGCGGCTTGG CTTGGAAGAC 
TCCATGATGC AGAACTGGAG TCTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sacm1l ( NM_030692.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109617.1 Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08368
Clone ID Related Accession (Same CDS sequence)	NM_030692.5 , NM_030692.4
Accession Version	NM_030692.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	phosphatidylinositide phosphatase SAC1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY127420.1, CJ173186.1, AK161111.1, CN539161.1 and CO044347.1. On Oct 27, 2017 this sequence version replaced NM_030692.4. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK154759.1, SRR1660817.136215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAGGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGAGTGTC CACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCC TCTGCTGTTA CAAGACCAAT ATTTGGGATA 
CTGGGCACAA TCCATCTGGT GGCAGGTAAT TATCTTGTTG TCATCACCAA AAAGATGAAA 
GTAGGTGAAT GTTTTAATCA TGCGGTCTGG AGAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTGCACTT GACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TATGGATGGA TTTTACTTTT CAACAACATA TGACTTGACT 
CATACTTTGC AGCGACTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCTTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GGGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCAGTGTTG CATGGCTTTA TTACTATGCA CTCATGTTCT 
ATTAATGGAA AATATTTTGA TTGGATTCTC ATCTCCCGAA GGAGTTGTTT CAGAGCTGGT 
GTTCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAGCAGATTG TGCACTACAG CGGGAACAGG GCTTCGTTTG TCCAGACTCG AGGATCAATA 
CCTATTTTCT GGTCACAGAG ACCAAATCTA AAGTACAAAC CACATCCACA GATCAGCAAA 
GTAGCAAATC ACATGGATGG TTTCCAGAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAGCAAGTAA TAATTAATCT GGTGAACCAC AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTCGCAAACA TGGTGTCCTC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGATTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CCGGCAAGGT GGTGACAAAC 
CAGGACGGAG TTTTCCGCAG CAACTGCATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGACGTTC CCTTCAGGCA CAGCTGCAGA GGCTAGGAGT TTTGCATGTG 
GGACAGAAGC TTGAAGAGCA AGATGAATTT GAGAAGACTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCG GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTA TTAATGGATG GCTTCAACTC ATTATTACGG 
TATTACAAGA ACAACTTTTC TGATGGATTT AGACAAGATT CCATAGACTT ATTTCTTGGA 
AATTATTCAG TGGATGAATT AGAATCTCAT AGCCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTG 
GTCCAGAAAG AAAAGATAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109617.1
CDS	138..1901
Translation	MAAAAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAVKKDVPPSAVTRPIFGILGTIHLVAGNYLVVITKKMKVGECFNHAVWRATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLSMDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPIFWSQRPNLKYKPHPQISKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFANMVSSLGSGMIRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGKVVTNQDGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKTYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLLMDGFNSLLRYYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID

Target ORF information:

RefSeq Version	NM_030692.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030692.5

ORF Insert Sequence:

ATGGCGGCCG CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAGGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGAGTGTC CACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCC TCTGCTGTTA CAAGACCAAT ATTTGGGATA 
CTGGGCACAA TCCATCTGGT GGCAGGTAAT TATCTTGTTG TCATCACCAA AAAGATGAAA 
GTAGGTGAAT GTTTTAATCA TGCGGTCTGG AGAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTGCACTT GACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TATGGATGGA TTTTACTTTT CAACAACATA TGACTTGACT 
CATACTTTGC AGCGACTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCTTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GGGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCAGTGTTG CATGGCTTTA TTACTATGCA CTCATGTTCT 
ATTAATGGAA AATATTTTGA TTGGATTCTC ATCTCCCGAA GGAGTTGTTT CAGAGCTGGT 
GTTCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAGCAGATTG TGCACTACAG CGGGAACAGG GCTTCGTTTG TCCAGACTCG AGGATCAATA 
CCTATTTTCT GGTCACAGAG ACCAAATCTA AAGTACAAAC CACATCCACA GATCAGCAAA 
GTAGCAAATC ACATGGATGG TTTCCAGAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAGCAAGTAA TAATTAATCT GGTGAACCAC AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTCGCAAACA TGGTGTCCTC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGATTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CCGGCAAGGT GGTGACAAAC 
CAGGACGGAG TTTTCCGCAG CAACTGCATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGACGTTC CCTTCAGGCA CAGCTGCAGA GGCTAGGAGT TTTGCATGTG 
GGACAGAAGC TTGAAGAGCA AGATGAATTT GAGAAGACTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCG GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTA TTAATGGATG GCTTCAACTC ATTATTACGG 
TATTACAAGA ACAACTTTTC TGATGGATTT AGACAAGATT CCATAGACTT ATTTCTTGGA 
AATTATTCAG TGGATGAATT AGAATCTCAT AGCCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTG 
GTCCAGAAAG AAAAGATAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sacm1l ( NM_030692.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109617.1 Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), mRNA.

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CloneID	OMu08368
Clone ID Related Accession (Same CDS sequence)	NM_030692.5 , NM_030692.4
Accession Version	NM_030692.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-23
Organism	Mus musculus(House mouse)
Product	phosphatidylinositide phosphatase SAC1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC132852.12. On Oct 19, 2007 this sequence version replaced NM_030692.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK122390.1, AK161111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCG CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAGGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGAGTGTC CACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCC TCTGCTGTTA CAAGACCAAT ATTTGGGATA 
CTGGGCACAA TCCATCTGGT GGCAGGTAAT TATCTTGTTG TCATCACCAA AAAGATGAAA 
GTAGGTGAAT GTTTTAATCA TGCGGTCTGG AGAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTGCACTT GACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TATGGATGGA TTTTACTTTT CAACAACATA TGACTTGACT 
CATACTTTGC AGCGACTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCTTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GGGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCAGTGTTG CATGGCTTTA TTACTATGCA CTCATGTTCT 
ATTAATGGAA AATATTTTGA TTGGATTCTC ATCTCCCGAA GGAGTTGTTT CAGAGCTGGT 
GTTCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAGCAGATTG TGCACTACAG CGGGAACAGG GCTTCGTTTG TCCAGACTCG AGGATCAATA 
CCTATTTTCT GGTCACAGAG ACCAAATCTA AAGTACAAAC CACATCCACA GATCAGCAAA 
GTAGCAAATC ACATGGATGG TTTCCAGAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAGCAAGTAA TAATTAATCT GGTGAACCAC AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTCGCAAACA TGGTGTCCTC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGATTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CCGGCAAGGT GGTGACAAAC 
CAGGACGGAG TTTTCCGCAG CAACTGCATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGACGTTC CCTTCAGGCA CAGCTGCAGA GGCTAGGAGT TTTGCATGTG 
GGACAGAAGC TTGAAGAGCA AGATGAATTT GAGAAGACTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCG GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTA TTAATGGATG GCTTCAACTC ATTATTACGG 
TATTACAAGA ACAACTTTTC TGATGGATTT AGACAAGATT CCATAGACTT ATTTCTTGGA 
AATTATTCAG TGGATGAATT AGAATCTCAT AGCCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTG 
GTCCAGAAAG AAAAGATAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109617.1
CDS	9..1772
Translation	MAAAAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAVKKDVPPSAVTRPIFGILGTIHLVAGNYLVVITKKMKVGECFNHAVWRATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLSMDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPIFWSQRPNLKYKPHPQISKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFANMVSSLGSGMIRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGKVVTNQDGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKTYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLLMDGFNSLLRYYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID

Target ORF information:

RefSeq Version	NM_030692.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030692.4

ORF Insert Sequence:

ATGGCGGCCG CAGCCTACGA GCATCTGAAG CTGCATATTA CACCTGAAAA GTTTTATGTG 
GAGGCTTGTG ATGATGGAGC AGATGATGTA CTCATCATTG ACCGAGTGTC CACAGAGGTC 
ACCCTTGCAG TCAAAAAAGA TGTTCCCCCC TCTGCTGTTA CAAGACCAAT ATTTGGGATA 
CTGGGCACAA TCCATCTGGT GGCAGGTAAT TATCTTGTTG TCATCACCAA AAAGATGAAA 
GTAGGTGAAT GTTTTAATCA TGCGGTCTGG AGAGCCACAG ATTTTGATGT TCTTTCTTAT 
AAGAAGACAA TGTTGCACTT GACTGATATT CAGTTACAAG ATAATAAAAC TTTCCTAGCA 
ATGCTAAACC ATGTCTTGAG TATGGATGGA TTTTACTTTT CAACAACATA TGACTTGACT 
CATACTTTGC AGCGACTATC TAACACGAGT CCAGAATTCC AAGAAATGAG TCTCTTAGAA 
AGGGCAGATC AGCGGTTTGT ATGGAACGGG CATCTGCTCA GGGAACTGTC TGCTCAGCCA 
GAGGTCCATC GGTTTGCTCT TCCAGTGTTG CATGGCTTTA TTACTATGCA CTCATGTTCT 
ATTAATGGAA AATATTTTGA TTGGATTCTC ATCTCCCGAA GGAGTTGTTT CAGAGCTGGT 
GTTCGTTATT ATGTGAGAGG AATTGATTCT GAAGGCCACG CAGCTAACTT TGTAGAAACA 
GAGCAGATTG TGCACTACAG CGGGAACAGG GCTTCGTTTG TCCAGACTCG AGGATCAATA 
CCTATTTTCT GGTCACAGAG ACCAAATCTA AAGTACAAAC CACATCCACA GATCAGCAAA 
GTAGCAAATC ACATGGATGG TTTCCAGAGG CATTTTGATT CACAAGTAAT TATTTATGGA 
AAGCAAGTAA TAATTAATCT GGTGAACCAC AAGGGATCAG AGAAACCACT TGAGCAAACA 
TTCGCAAACA TGGTGTCCTC CTTGGGAAGT GGGATGATCA GATATATTGC CTTTGACTTC 
CATAAGGAGT GTAAAAATAT GAGATGGGAT CGATTGAGTA TTTTATTGGA TCAAGTAGCA 
GAAATGCAGG ATGAATTAAG TTATTTTTTA GTAGACTCTG CCGGCAAGGT GGTGACAAAC 
CAGGACGGAG TTTTCCGCAG CAACTGCATG GATTGTCTAG ACAGAACGAA TGTGATACAG 
AGTTTATTAG CTCGACGTTC CCTTCAGGCA CAGCTGCAGA GGCTAGGAGT TTTGCATGTG 
GGACAGAAGC TTGAAGAGCA AGATGAATTT GAGAAGACTT ACAAAAATGC CTGGGCCGAT 
AATGCTAATG CTTGTGCCAA ACAGTATGCG GGAACCGGTG CCTTGAAGAC TGACTTTACA 
AGAACTGGAA AAAGAACTCA GTTGGGACTA TTAATGGATG GCTTCAACTC ATTATTACGG 
TATTACAAGA ACAACTTTTC TGATGGATTT AGACAAGATT CCATAGACTT ATTTCTTGGA 
AATTATTCAG TGGATGAATT AGAATCTCAT AGCCCTTTAA GTGTTCCAAG GGACTGGAAA 
TTCCTGGCGT TGCCTATCAT CATGGTTGTT GCCTTTTCAA TGTGCATCAT CTGTTTGCTT 
ATGGCTGGTG ACACTTGGAC AGAAACACTG GCATATGTCC TCTTCTGGGG AGTTGCAAGC 
ATTGGAACAT TTTTTATTAT TCTTTACAAT GGCAAAGATT TTGTTGATGC TCCCAGACTG 
GTCCAGAAAG AAAAGATAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sacm1l ( XM_017313703.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169192.1 Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant X1, mRNA.

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CloneID	OMu83007
Clone ID Related Accession (Same CDS sequence)	XM_017313703.1
Accession Version	XM_017313703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositide phosphatase SAC1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGGC TTGTGATGAT GGAGCAGATG ATGTACTCAT CATTGACCGA GTGTCCACAG 
AGGTCACCCT TGCAGTCAAA AAAGATGTTC CCCCCTCTGC TGTTACAAGA CCAATATTTG 
GGATACTGGG CACAATCCAT CTGGTGGCAG TTACAAGATA ATAAAACTTT CCTAGCAATG 
CTAAACCATG TCTTGAGTAT GGATGGATTT TACTTTTCAA CAACATATGA CTTGACTCAT 
ACTTTGCAGC GACTATCTAA CACGAGTCCA GAATTCCAAG AAATGAGTCT CTTAGAAAGG 
GCAGATCAGC GGTTTGTATG GAACGGGCAT CTGCTCAGGG AACTGTCTGC TCAGCCAGAG 
GTCCATCGGT TTGCTCTTCC AGTGTTGCAT GGCTTTATTA CTATGCACTC ATGTTCTATT 
AATGGAAAAT ATTTTGATTG GATTCTCATC TCCCGAAGGA GTTGTTTCAG AGCTGGTGTT 
CGTTATTATG TGAGAGGAAT TGATTCTGAA GGCCACGCAG CTAACTTTGT AGAAACAGAG 
CAGATTGTGC ACTACAGCGG GAACAGGGCT TCGTTTGTCC AGACTCGAGG ATCAATACCT 
ATTTTCTGGT CACAGAGACC AAATCTAAAG TACAAACCAC ATCCACAGAT CAGCAAAGTA 
GCAAATCACA TGGATGGTTT CCAGAGGCAT TTTGATTCAC AAGTAATTAT TTATGGAAAG 
CAAGTAATAA TTAATCTGGT GAACCACAAG GGATCAGAGA AACCACTTGA GCAAACATTC 
GCAAACATGG TGTCCTCCTT GGGAAGTGGG ATGATCAGAT ATATTGCCTT TGACTTCCAT 
AAGGAGTGTA AAAATATGAG ATGGGATCGA TTGAGTATTT TATTGGATCA AGTAGCAGAA 
ATGCAGGATG AATTAAGTTA TTTTTTAGTA GACTCTGCCG GCAAGGTGGT GACAAACCAG 
GACGGAGTTT TCCGCAGCAA CTGCATGGAT TGTCTAGACA GAACGAATGT GATACAGAGT 
TTATTAGCTC GACGTTCCCT TCAGGCACAG CTGCAGAGGC TAGGAGTTTT GCATGTGGGA 
CAGAAGCTTG AAGAGCAAGA TGAATTTGAG AAGACTTACA AAAATGCCTG GGCCGATAAT 
GCTAATGCTT GTGCCAAACA GTATGCGGGA ACCGGTGCCT TGAAGACTGA CTTTACAAGA 
ACTGGAAAAA GAACTCAGTT GGGACTATTA ATGGATGGCT TCAACTCATT ATTACGGTAT 
TACAAGAACA ACTTTTCTGA TGGATTTAGA CAAGATTCCA TAGACTTATT TCTTGGAAAT 
TATTCAGTGG ATGAATTAGA ATCTCATAGC CCTTTAAGTG TTCCAAGGGA CTGGAAATTC 
CTGGCGTTGC CTATCATCAT GGTTGTTGCC TTTTCAATGT GCATCATCTG TTTGCTTATG 
GCTGGTGACA CTTGGACAGA AACACTGGCA TATGTCCTCT TCTGGGGAGT TGCAAGCATT 
GGAACATTTT TTATTATTCT TTACAATGGC AAAGATTTTG TTGATGCTCC CAGACTGGTC 
CAGAAAGAAA AGATAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169192.1
CDS	1..1581
Translation	MWRLVMMEQMMYSSLTECPQRSPLQSKKMFPPLLLQDQYLGYWAQSIWWQLQDNKTFLAMLNHVLSMDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPIFWSQRPNLKYKPHPQISKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFANMVSSLGSGMIRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGKVVTNQDGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKTYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLLMDGFNSLLRYYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID

Target ORF information:

RefSeq Version	XM_017313703.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313703.1

ORF Insert Sequence:

ATGTGGAGGC TTGTGATGAT GGAGCAGATG ATGTACTCAT CATTGACCGA GTGTCCACAG 
AGGTCACCCT TGCAGTCAAA AAAGATGTTC CCCCCTCTGC TGTTACAAGA CCAATATTTG 
GGATACTGGG CACAATCCAT CTGGTGGCAG TTACAAGATA ATAAAACTTT CCTAGCAATG 
CTAAACCATG TCTTGAGTAT GGATGGATTT TACTTTTCAA CAACATATGA CTTGACTCAT 
ACTTTGCAGC GACTATCTAA CACGAGTCCA GAATTCCAAG AAATGAGTCT CTTAGAAAGG 
GCAGATCAGC GGTTTGTATG GAACGGGCAT CTGCTCAGGG AACTGTCTGC TCAGCCAGAG 
GTCCATCGGT TTGCTCTTCC AGTGTTGCAT GGCTTTATTA CTATGCACTC ATGTTCTATT 
AATGGAAAAT ATTTTGATTG GATTCTCATC TCCCGAAGGA GTTGTTTCAG AGCTGGTGTT 
CGTTATTATG TGAGAGGAAT TGATTCTGAA GGCCACGCAG CTAACTTTGT AGAAACAGAG 
CAGATTGTGC ACTACAGCGG GAACAGGGCT TCGTTTGTCC AGACTCGAGG ATCAATACCT 
ATTTTCTGGT CACAGAGACC AAATCTAAAG TACAAACCAC ATCCACAGAT CAGCAAAGTA 
GCAAATCACA TGGATGGTTT CCAGAGGCAT TTTGATTCAC AAGTAATTAT TTATGGAAAG 
CAAGTAATAA TTAATCTGGT GAACCACAAG GGATCAGAGA AACCACTTGA GCAAACATTC 
GCAAACATGG TGTCCTCCTT GGGAAGTGGG ATGATCAGAT ATATTGCCTT TGACTTCCAT 
AAGGAGTGTA AAAATATGAG ATGGGATCGA TTGAGTATTT TATTGGATCA AGTAGCAGAA 
ATGCAGGATG AATTAAGTTA TTTTTTAGTA GACTCTGCCG GCAAGGTGGT GACAAACCAG 
GACGGAGTTT TCCGCAGCAA CTGCATGGAT TGTCTAGACA GAACGAATGT GATACAGAGT 
TTATTAGCTC GACGTTCCCT TCAGGCACAG CTGCAGAGGC TAGGAGTTTT GCATGTGGGA 
CAGAAGCTTG AAGAGCAAGA TGAATTTGAG AAGACTTACA AAAATGCCTG GGCCGATAAT 
GCTAATGCTT GTGCCAAACA GTATGCGGGA ACCGGTGCCT TGAAGACTGA CTTTACAAGA 
ACTGGAAAAA GAACTCAGTT GGGACTATTA ATGGATGGCT TCAACTCATT ATTACGGTAT 
TACAAGAACA ACTTTTCTGA TGGATTTAGA CAAGATTCCA TAGACTTATT TCTTGGAAAT 
TATTCAGTGG ATGAATTAGA ATCTCATAGC CCTTTAAGTG TTCCAAGGGA CTGGAAATTC 
CTGGCGTTGC CTATCATCAT GGTTGTTGCC TTTTCAATGT GCATCATCTG TTTGCTTATG 
GCTGGTGACA CTTGGACAGA AACACTGGCA TATGTCCTCT TCTGGGGAGT TGCAAGCATT 
GGAACATTTT TTATTATTCT TTACAATGGC AAAGATTTTG TTGATGCTCC CAGACTGGTC 
CAGAAAGAAA AGATAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Nucleic acids research42(14)8928-38(2014 Aug)
Loughran G,Chou MY,Ivanov IP,Jungreis I,Kellis M,Kiran AM,Baranov PV,Atkins JF

A novel probe for phosphatidylinositol 4-phosphate reveals multiple pools beyond the Golgi.
The Journal of cell biology205(1)113-26(2014 Apr)
Hammond GR,Machner MP,Balla T

High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes.
Neuron74(4)621-33(2012 May)
Schwenk J,Harmel N,Brechet A,Zolles G,Berkefeld H,M?ller CS,Bildl W,Baehrens D,H?ber B,Kulik A,Kl?cker N,Schulte U,Fakler B

Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease.
Physiological genomics39(3)160-8(2009 Nov)
Tkatchenko TV,Moreno-Rodriguez RA,Conway SJ,Molkentin JD,Markwald RR,Tkatchenko AV

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Sacm1l cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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