Cacna1f cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1f
Entrez Gene ID	114493
Full Name	calcium voltage-gated channel subunit alpha1 F
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 F Names voltage-dependent L-type calcium channel subunit alpha-1F calcium channel, voltage-dependent, L type, alpha 1F subunit calcium channel, voltage-dependent, alpha 1F subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq12
Summary	mutation of the human homolog causes incomplete X-linked congentital stationary night blindness (CSNB2) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601894.1	XP_017457383.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
XM_017601895.1	XP_017457384.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X3
XM_017601896.1	XP_017457385.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X4
XM_006256698.3	XP_006256760.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
NM_053701.1	NP_446153.1	voltage-dependent L-type calcium channel subunit alpha-1F

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1F	NP_005174.2
	CACNA1F	XP_001106163.2
Canis lupus familiaris (dog)	CACNA1F	XP_005642146.1
Danio rerio (zebrafish)	LOC559964	XP_003199134.2
Bos taurus (cattle)	CACNA1F	NP_001192592.1
Mus musculus (house mouse)	Cacna1f	NP_062528.2
Danio rerio (zebrafish)	LOC793708	XP_001333514.5
Xenopus tropicalis (tropical clawed frog)	cacna1f	XP_002941018.2
Rattus norvegicus (Norway rat)	Cacna1f	NP_446153.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of C-terminal Splice Variants of Cav1.4 Ca2+ Channels in Human Retina.
Haeseleer F, Williams B, Lee A
The Journal of biological chemistry291(30)15663-73(2016 07)

Cacna1f gene decreased contractility of skeletal muscle in rat model with congenital stationary night blindness.
An J, Zhang L, Jiao B, Lu F, Xia F, Yu Z, Zhang Z
Gene562(2)210-9(2015 May)

Visual signal pathway reorganization in the Cacna1f mutant rat model.
Tao Y, Chen T, Liu B, Xue JH, Zhang L, Xia F, Pang JJ, Zhang ZM
Investigative ophthalmology & visual science54(3)1988-97(2013 Mar)

Behavioral phenotypic properties of a natural occurring rat model of congenital stationary night blindness with Cacna1f mutation.
An J, Wang L, Guo Q, Li L, Xia F, Zhang Z
Journal of neurogenetics26(3-4)363-73(2012 Sep)

Retinal horizontal cells reduced in a rat model of congenital stationary night blindness.
Zheng L, Yan Y, An J, Zhang L, Liu W, Xia F, Zhang Z
Neuroscience letters521(1)26-30(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The congenital stationary night blindness Cacna1f mutation reduces the function of slow-twitch skeletal muscle.
Title: Cacna1f gene decreased contractility of skeletal muscle in rat model with congenital stationary night blindness.

Using MEA recording, we provide evidences of functional changes for visual signal pathway plasticity in the Cacna1f mutated retinas.
Title: Visual signal pathway reorganization in the Cacna1f mutant rat model.

This study demonistrated that the animal behavior in rat model of congenital stationary night blindness with Cacna1f mutation.
Title: Behavioral phenotypic properties of a natural occurring rat model of congenital stationary night blindness with Cacna1f mutation.

A c.2941C>T (p.R981Stop) mutation in Cacna1f was found in affected rats.
Title: A naturally-occurring mutation in Cacna1f in a rat model of congenital stationary night blindness.

The following Cacna1f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa62991	XM_017601895.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa39597	XM_006256698.3 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa10353	NM_053701.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1700.30 $2429.00
ORa62990	XM_017601894.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa62992	XM_017601896.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1f ( XM_017601895.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457384.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa62991
Clone ID Related Accession (Same CDS sequence)	XM_017601895.1
Accession Version	XM_017601895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATG CCATGGGGTA TGAGCTACCT TGGGTGTACT TTGTGAGCCT CGTCATCTTT 
GGGTCTTTCT TTGTCCTCAA CCTTGTGCTT GGAGTCCTAA GTGGGGAGTT CTCCAAGGAA 
AGAGAAAAGG CAAAAGCCCG AGGTGACTTT CAGAAGCTTC GGGAGAAGCA GCAGATGGAA 
GAGGACCTTC GGGGCTACCT GGACTGGATC ACACAGGCTG AGGAGTTGGA CCTTCATGAC 
CCCTCAGTAG ACGGCAACTT GGCTTCTCTT GCTGAAGAGG GACGGGCAGG CCATCGACCA 
CAACTGTCAG AGCTGACCAA TAGGAGGCGC GGACGACTGC GTTGGTTCAG CCACTCCACT 
CGCTCCACAC ACTCCACCAG CAGTCATGCC AGCCTTCCAG CCAGTGACAC TGGTTCCATA 
ACAGACACCC CCGGAGATGA GGATGAAGAG GAGGGGACCA TGGCCAGCTG TACACTCTGC 
CTAAACAAGA TTATGAAAAC CAAGGTCTGC CGCCACTTCC GCCGAGCCAA CCGTGGTCTC 
CGTGCACGCT GCCGCCGGGC AGTCAAGTCC AATGCCTGCT ACTGGGCTGT ATTGCTGCTC 
GTCTTCCTCA ACACGTTGAC CATCGCTTCT GAGCACCATG GGCAGCCAGT GTGGCTCACC 
CAGACCCAAG AATATGCCAA CAAGGTTCTG CTCTGCCTCT TCACCGTGGA GATGCTTCTC 
AAACTGTACG GCCTGGGCCC CTCTGTCTAC GTTGCCTCTT TTTTCAACCG CTTTGACTGT 
TTCGTGGTCT GTGGGGGCAT CCTAGAAACG ACGTTGGTGG AGGTGGGGGC CATGCAGCCC 
CTCGGCATCT CAGTGCTCCG ATGTGTGCGT CTCCTCAGGA TCTTCAAGGT CACCAGGCAC 
TGGGCATCCC TCAGCAATCT GGTGGCATCG CTGCTCAACT CCATGAAGTC CATCGCCTCC 
TTGCTGCTTC TCCTCTTTCT CTTTATCATC ATCTTCTCCC TTCTTGGCAT GCAGCTGTTT 
GGGGGCAAGT TCAACTTTGA CCAGACCCAC ACTAAGAGAA GCACCTTTGA TACCTTCCCC 
CAAGCCCTCC TCACCGTCTT TCAGATCCTG ACTGGTGAGG ATTGGAATGT AGTCATGTAT 
GACGGTATCA TGGCCTACGG TGGTCCCTTC TTCCCAGGGA TGCTGGTGTG TGTTTATTTC 
ATCATCCTCT TCATCTGTGG CAACTACATC CTACTGAACG TGTTTCTTGC CATTGCTGTG 
GATAACCTAG CCAGCGGGGA TGCGGGTGCT GCCAAAGATA AGGGCAGAGA GAAGAGCAGT 
GAAGGAAACC CTCCCCAAGA GAACAAAGTA TTGGTGCCTG GTGGAGAAAA TGAGGACACA 
GAGGGCACAA AAAGTGAAGG AGCAGCACCA GGCATGGAGG AAGAGGAGGA AGAAGAGGAG 
GAGGAGGAGG AAGAAAATGG TGCAGGACAT GTGGAACTCC TGCAGGAAGT TGTACCCAAG 
GAGAAGGTGG TACCCATCCC TGAAGGCAGT GCCTTCTTCT GCCTCAGCCA AACCAACCCG 
CTTCGCAAGG CCTGCCACAC TCTCATACAC CATCACGTGT TCACCAGTCT CATCCTGGTG 
TTCATCATCC TCAGTAGTGT GTCCCTGGCT GCTGAGGACC CCATCAGAGC CCACTCCTTC 
CGCAACCATA TTCTGGGGTA CTTCGATTAT GCCTTCACTT CCATTTTCAC CGTGGAGATT 
CTATTAAAGA TGACGGTGTT TGGGGCTTTC CTGCACCAAG GTTCTTTCTG CCGTAGCTGG 
TTCAATCTGT TGGATCTGCT TGTGGTCAGT GTGTCCCTCA TCTCCTTTGG CATCCACTCC 
AGTGCCATCT CAGTTGTGAA GATTCTCCGA GTCCTCCGAG TCCTGCGGCC TCTCCGAGCC 
ATTAACCGAG CCAAGGGACT CAAGCATGTG GTGCAGTGTG TGTTTGTGGC CATCCGGACC 
ATTGGAAACA TCATGATTGT CACCACCCTC TTGCAGTTCA TGTTCGCCTG CATTGGTGTT 
CAGCTGTTCA AGGGAAAATT CTACAGTTGC ACTGATGAAG CCAAACACAC CCTGAAAGAA 
TGCAAGGGCT CCTTCCTCAT CTACCCTGAT GGAGATGTGT CACGACCTTT GGTCCGGGAA 
CGCCTCTGGG TCAACAGTGA TTTCAACTTT GACAACGTCC TTTCAGCCAT GATGGCCCTG 
TTCACTGTCT CTACCTTTGA AGGCTGGCCT GCGCTACTAT ACAAAGCCAT AGATGCACAC 
GCTGAAGACG AGGGCCCTAT CTACAATTAC CATGTGGAGA TATCAGTATT CTTCATTGTC 
TACATCATCA TCATCGCCTT CTTCATGATG AACATCTTTG TGGGATTTGT CATCATCACA 
TTCCGTGCCC AGGGAGAGCA GGAATATCAA AACTGTGAAC TGGACAAGAA CCAGCGCCAG 
TGTGTGGAAT ATGCACTCAA AGCTCAGCCA CTCCGCCGAT ACATCCCCAA GAATCCACAT 
CAGTACCGCG TGTGGGCCAC AGTGAACTCA GCCGCCTTTG AGTACCTCAT GTTTCTGCTC 
ATCCTCCTCA ACACAGTTGC CCTGGCCATG CAGCACTATG AGCAGACGGC TCCCTTTAAC 
TATGCCATGG ACATACTCAA CATGGTCTTC ACTGGCCTCT TCACCGTTGA GATGGTGCTC 
AAAATCATTG CCTTTAAACC CAAGCATTAC TTTGCCGATG CCTGGAATAC ATTCGATGCT 
CTTATTGTAG TGGGCAGCGT AGTCGACATT GCCGTCACAG AAGTCAATAA TGGAGGCCAT 
CTTGGTGAGA GCTCAGAGGA CAGCTCCCGC ATATCTATCA CGTTCTTTCG CCTCTTCCGG 
GTCATGAGGC TGGTGAAGCT GCTCAGTAAG GGTGAGGGGA TCCGCACACT GCTCTGGACA 
TTCATCAAGT CTTTCCAGGC CTTGCCCTAT GTGGCACTTC TTATAGCAAT GATATTCTTC 
ATATATGCAG TCATTGGCAT GCAGATGTTT GGCAAAGTGG CTCTTCAGGA CGGCACACAG 
ATAAATCGAA ACAATAATTT CCAGACCTTT CCGCAGGCTG TGCTGCTTCT GTTCAGGTGT 
GCCACTGGTG AGGCATGGCA AGAGATAATG CTAGCCAGCC TTCCAGGAAA TCGGTGTGAC 
CCTGAGTCTG ACTTTGGCCC AGGCGAGGAA TTTACCTGTG GTAGCAATTT TGCCATTGTC 
TACTTTATCA GCTTCTTCAT GCTCTGTGCC TTCCTGATTA TAAATCTCTT TGTGGCTGTA 
ATCATGGATA ACTTTGATTA CCTAACCAGA GATTGGTCTA TTCTGGGACC CCACCACCTC 
GATGAATTCA AGAGGATCTG GTCTGAATAT GACCCCGGAG CCAAGGGCCG CATCAAGCAC 
TTGGATGTGG TTGCCCTGCT GAGACGCATC CAGCCTCCAC TGGGATTTGG AAAGCTGTGC 
CCACACCGAG TGGCCTGCAA GAGACTGGTG GCCATGAATG TTCCCCTCAA CTCAGACGGA 
ACAGTGACAT TCAACGCGAC ACTCTTTGCC CTGGTGCGGA CATCCCTGAG GATCAAGACA 
GAAGGGAACC TGGATCAAGC CAACCAGGAG CTTCGGATGG TCATCAAAAA GATCTGGAAG 
CGGATAAAGC AGAAATTGTT AGATGAGGTC ATCCCCCCTC CAGATGAGGA GGAGGTCACT 
GTGGGAAAAT TCTATGCCAC ATTTCTCATC CAAGATTATT TCCGAAAATT CCGGAGAAGG 
AAAGAAAAGG GGCTACTAGG AGCAGATGCC CCAACAAGCA CATCCTCTGT CCTCCAGGCT 
GGTCTAAGGA GCCTACAGGA CTTGGGTCCT GAGATCCGAC AGGCCCTCAC CTATGACACT 
GAGGAAGAAG AAGAAGAAGA AGAGGCCGCA GGTCAGGAAG CTGAGGAGGA GGAAGCTGAG 
AACAACCCAG AAACATACAA AGACTCCATA GACTCCCAGC CCCAAGCTCA ATGGAACTCG 
AGGATTTCGG TGTCTCTACC AGTTAAGGAG AAACTCCCAG ATTCTCTTTC AACTGGGCCT 
AGTGATGATG ATGGAGTGGC TCCCAACTCC AGGCAACCCA GTGGGCTACA GGCTGGATCC 
CAACCCCACA GGAGAAGCTC TGGGGTTTTC ATGTTCACTA TCCCAGAAGA AGGAAGTACT 
CAGCTCAAGG GAGTTCAAGG GCAAGACAAT CAGAATGAGG AACAGGAAGT CCCTGACTGG 
ACCCCCAACC TGGATGAGCA GGCAGGGATG CCCTCCAACC CAGTCCTTTT ACCACCTCAC 
TGGTCCCAGC AACATGTAAA TGGGCACCAT GTACCACGCC GACGTTTGCT ACCCCCCACG 
CCTGCAGGTC GGAAGCCCTC CTTCACCATC CAGTGTCTGC AACGCCAGGG CAGTTGTGAG 
GATTTGCCCA TCCCAGGCAC CTACCATCGT GGACGGACCT CAGGACCAGG CAGGGCTCAG 
GGTTCCTGGG CAGCCCCTCC TCAGAAGGGT CGACTGCTCT ATGCCCCCCT GTTGCTGGTG 
GAGGAATCTA CAGTGGGTGA AGGATACCTT GGCAAGCTCG GCGGCCCACT GCGTACCTTC 
ACTTGTCTGC AAGTGCCTGG AGCTCCTTCA GATCCCAGTC ACCGCAAGAG GGGCAGTGCT 
GACAGTTTGG TGGAGGCTGT GCTTATCTCC GAAGGCCTAG GCCTCTTTGC CCAAGACCCA 
CGATTTGTGG CCCTGGCCAA GCAAGAGATT GCAGATGCAT GTCACCTGAC CCTGGATGAG 
ATGGACAGTG CTGCCAGTGA CCTGCTGGCA CAGAGAACCA CCTCCCTTTA CAGTGACGAG 
GAGTCTATTC TCTCCCGCTT TGATGAAGAG GACCTGGGAG ATGAGATGGC CTGTGTCCAT 
GCCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457384.1
CDS	91..5019
Translation	MQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSITDTPGDEDEEEGTMASCTLCLNKIMKTKVCRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGAAKDKGREKSSEGNPPQENKVLVPGGENEDTEGTKSEGAAPGMEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHVFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHQGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAHAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLRIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGADAPTSTSSVLQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAAGQEAEEEEAENNPETYKDSIDSQPQAQWNSRISVSLPVKEKLPDSLSTGPSDDDGVAPNSRQPSGLQAGSQPHRRSSGVFMFTIPEEGSTQLKGVQGQDNQNEEQEVPDWTPNLDEQAGMPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPGRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAPSDPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_017601895.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601895.1

ORF Insert Sequence:

ATGCAGGATG CCATGGGGTA TGAGCTACCT TGGGTGTACT TTGTGAGCCT CGTCATCTTT 
GGGTCTTTCT TTGTCCTCAA CCTTGTGCTT GGAGTCCTAA GTGGGGAGTT CTCCAAGGAA 
AGAGAAAAGG CAAAAGCCCG AGGTGACTTT CAGAAGCTTC GGGAGAAGCA GCAGATGGAA 
GAGGACCTTC GGGGCTACCT GGACTGGATC ACACAGGCTG AGGAGTTGGA CCTTCATGAC 
CCCTCAGTAG ACGGCAACTT GGCTTCTCTT GCTGAAGAGG GACGGGCAGG CCATCGACCA 
CAACTGTCAG AGCTGACCAA TAGGAGGCGC GGACGACTGC GTTGGTTCAG CCACTCCACT 
CGCTCCACAC ACTCCACCAG CAGTCATGCC AGCCTTCCAG CCAGTGACAC TGGTTCCATA 
ACAGACACCC CCGGAGATGA GGATGAAGAG GAGGGGACCA TGGCCAGCTG TACACTCTGC 
CTAAACAAGA TTATGAAAAC CAAGGTCTGC CGCCACTTCC GCCGAGCCAA CCGTGGTCTC 
CGTGCACGCT GCCGCCGGGC AGTCAAGTCC AATGCCTGCT ACTGGGCTGT ATTGCTGCTC 
GTCTTCCTCA ACACGTTGAC CATCGCTTCT GAGCACCATG GGCAGCCAGT GTGGCTCACC 
CAGACCCAAG AATATGCCAA CAAGGTTCTG CTCTGCCTCT TCACCGTGGA GATGCTTCTC 
AAACTGTACG GCCTGGGCCC CTCTGTCTAC GTTGCCTCTT TTTTCAACCG CTTTGACTGT 
TTCGTGGTCT GTGGGGGCAT CCTAGAAACG ACGTTGGTGG AGGTGGGGGC CATGCAGCCC 
CTCGGCATCT CAGTGCTCCG ATGTGTGCGT CTCCTCAGGA TCTTCAAGGT CACCAGGCAC 
TGGGCATCCC TCAGCAATCT GGTGGCATCG CTGCTCAACT CCATGAAGTC CATCGCCTCC 
TTGCTGCTTC TCCTCTTTCT CTTTATCATC ATCTTCTCCC TTCTTGGCAT GCAGCTGTTT 
GGGGGCAAGT TCAACTTTGA CCAGACCCAC ACTAAGAGAA GCACCTTTGA TACCTTCCCC 
CAAGCCCTCC TCACCGTCTT TCAGATCCTG ACTGGTGAGG ATTGGAATGT AGTCATGTAT 
GACGGTATCA TGGCCTACGG TGGTCCCTTC TTCCCAGGGA TGCTGGTGTG TGTTTATTTC 
ATCATCCTCT TCATCTGTGG CAACTACATC CTACTGAACG TGTTTCTTGC CATTGCTGTG 
GATAACCTAG CCAGCGGGGA TGCGGGTGCT GCCAAAGATA AGGGCAGAGA GAAGAGCAGT 
GAAGGAAACC CTCCCCAAGA GAACAAAGTA TTGGTGCCTG GTGGAGAAAA TGAGGACACA 
GAGGGCACAA AAAGTGAAGG AGCAGCACCA GGCATGGAGG AAGAGGAGGA AGAAGAGGAG 
GAGGAGGAGG AAGAAAATGG TGCAGGACAT GTGGAACTCC TGCAGGAAGT TGTACCCAAG 
GAGAAGGTGG TACCCATCCC TGAAGGCAGT GCCTTCTTCT GCCTCAGCCA AACCAACCCG 
CTTCGCAAGG CCTGCCACAC TCTCATACAC CATCACGTGT TCACCAGTCT CATCCTGGTG 
TTCATCATCC TCAGTAGTGT GTCCCTGGCT GCTGAGGACC CCATCAGAGC CCACTCCTTC 
CGCAACCATA TTCTGGGGTA CTTCGATTAT GCCTTCACTT CCATTTTCAC CGTGGAGATT 
CTATTAAAGA TGACGGTGTT TGGGGCTTTC CTGCACCAAG GTTCTTTCTG CCGTAGCTGG 
TTCAATCTGT TGGATCTGCT TGTGGTCAGT GTGTCCCTCA TCTCCTTTGG CATCCACTCC 
AGTGCCATCT CAGTTGTGAA GATTCTCCGA GTCCTCCGAG TCCTGCGGCC TCTCCGAGCC 
ATTAACCGAG CCAAGGGACT CAAGCATGTG GTGCAGTGTG TGTTTGTGGC CATCCGGACC 
ATTGGAAACA TCATGATTGT CACCACCCTC TTGCAGTTCA TGTTCGCCTG CATTGGTGTT 
CAGCTGTTCA AGGGAAAATT CTACAGTTGC ACTGATGAAG CCAAACACAC CCTGAAAGAA 
TGCAAGGGCT CCTTCCTCAT CTACCCTGAT GGAGATGTGT CACGACCTTT GGTCCGGGAA 
CGCCTCTGGG TCAACAGTGA TTTCAACTTT GACAACGTCC TTTCAGCCAT GATGGCCCTG 
TTCACTGTCT CTACCTTTGA AGGCTGGCCT GCGCTACTAT ACAAAGCCAT AGATGCACAC 
GCTGAAGACG AGGGCCCTAT CTACAATTAC CATGTGGAGA TATCAGTATT CTTCATTGTC 
TACATCATCA TCATCGCCTT CTTCATGATG AACATCTTTG TGGGATTTGT CATCATCACA 
TTCCGTGCCC AGGGAGAGCA GGAATATCAA AACTGTGAAC TGGACAAGAA CCAGCGCCAG 
TGTGTGGAAT ATGCACTCAA AGCTCAGCCA CTCCGCCGAT ACATCCCCAA GAATCCACAT 
CAGTACCGCG TGTGGGCCAC AGTGAACTCA GCCGCCTTTG AGTACCTCAT GTTTCTGCTC 
ATCCTCCTCA ACACAGTTGC CCTGGCCATG CAGCACTATG AGCAGACGGC TCCCTTTAAC 
TATGCCATGG ACATACTCAA CATGGTCTTC ACTGGCCTCT TCACCGTTGA GATGGTGCTC 
AAAATCATTG CCTTTAAACC CAAGCATTAC TTTGCCGATG CCTGGAATAC ATTCGATGCT 
CTTATTGTAG TGGGCAGCGT AGTCGACATT GCCGTCACAG AAGTCAATAA TGGAGGCCAT 
CTTGGTGAGA GCTCAGAGGA CAGCTCCCGC ATATCTATCA CGTTCTTTCG CCTCTTCCGG 
GTCATGAGGC TGGTGAAGCT GCTCAGTAAG GGTGAGGGGA TCCGCACACT GCTCTGGACA 
TTCATCAAGT CTTTCCAGGC CTTGCCCTAT GTGGCACTTC TTATAGCAAT GATATTCTTC 
ATATATGCAG TCATTGGCAT GCAGATGTTT GGCAAAGTGG CTCTTCAGGA CGGCACACAG 
ATAAATCGAA ACAATAATTT CCAGACCTTT CCGCAGGCTG TGCTGCTTCT GTTCAGGTGT 
GCCACTGGTG AGGCATGGCA AGAGATAATG CTAGCCAGCC TTCCAGGAAA TCGGTGTGAC 
CCTGAGTCTG ACTTTGGCCC AGGCGAGGAA TTTACCTGTG GTAGCAATTT TGCCATTGTC 
TACTTTATCA GCTTCTTCAT GCTCTGTGCC TTCCTGATTA TAAATCTCTT TGTGGCTGTA 
ATCATGGATA ACTTTGATTA CCTAACCAGA GATTGGTCTA TTCTGGGACC CCACCACCTC 
GATGAATTCA AGAGGATCTG GTCTGAATAT GACCCCGGAG CCAAGGGCCG CATCAAGCAC 
TTGGATGTGG TTGCCCTGCT GAGACGCATC CAGCCTCCAC TGGGATTTGG AAAGCTGTGC 
CCACACCGAG TGGCCTGCAA GAGACTGGTG GCCATGAATG TTCCCCTCAA CTCAGACGGA 
ACAGTGACAT TCAACGCGAC ACTCTTTGCC CTGGTGCGGA CATCCCTGAG GATCAAGACA 
GAAGGGAACC TGGATCAAGC CAACCAGGAG CTTCGGATGG TCATCAAAAA GATCTGGAAG 
CGGATAAAGC AGAAATTGTT AGATGAGGTC ATCCCCCCTC CAGATGAGGA GGAGGTCACT 
GTGGGAAAAT TCTATGCCAC ATTTCTCATC CAAGATTATT TCCGAAAATT CCGGAGAAGG 
AAAGAAAAGG GGCTACTAGG AGCAGATGCC CCAACAAGCA CATCCTCTGT CCTCCAGGCT 
GGTCTAAGGA GCCTACAGGA CTTGGGTCCT GAGATCCGAC AGGCCCTCAC CTATGACACT 
GAGGAAGAAG AAGAAGAAGA AGAGGCCGCA GGTCAGGAAG CTGAGGAGGA GGAAGCTGAG 
AACAACCCAG AAACATACAA AGACTCCATA GACTCCCAGC CCCAAGCTCA ATGGAACTCG 
AGGATTTCGG TGTCTCTACC AGTTAAGGAG AAACTCCCAG ATTCTCTTTC AACTGGGCCT 
AGTGATGATG ATGGAGTGGC TCCCAACTCC AGGCAACCCA GTGGGCTACA GGCTGGATCC 
CAACCCCACA GGAGAAGCTC TGGGGTTTTC ATGTTCACTA TCCCAGAAGA AGGAAGTACT 
CAGCTCAAGG GAGTTCAAGG GCAAGACAAT CAGAATGAGG AACAGGAAGT CCCTGACTGG 
ACCCCCAACC TGGATGAGCA GGCAGGGATG CCCTCCAACC CAGTCCTTTT ACCACCTCAC 
TGGTCCCAGC AACATGTAAA TGGGCACCAT GTACCACGCC GACGTTTGCT ACCCCCCACG 
CCTGCAGGTC GGAAGCCCTC CTTCACCATC CAGTGTCTGC AACGCCAGGG CAGTTGTGAG 
GATTTGCCCA TCCCAGGCAC CTACCATCGT GGACGGACCT CAGGACCAGG CAGGGCTCAG 
GGTTCCTGGG CAGCCCCTCC TCAGAAGGGT CGACTGCTCT ATGCCCCCCT GTTGCTGGTG 
GAGGAATCTA CAGTGGGTGA AGGATACCTT GGCAAGCTCG GCGGCCCACT GCGTACCTTC 
ACTTGTCTGC AAGTGCCTGG AGCTCCTTCA GATCCCAGTC ACCGCAAGAG GGGCAGTGCT 
GACAGTTTGG TGGAGGCTGT GCTTATCTCC GAAGGCCTAG GCCTCTTTGC CCAAGACCCA 
CGATTTGTGG CCCTGGCCAA GCAAGAGATT GCAGATGCAT GTCACCTGAC CCTGGATGAG 
ATGGACAGTG CTGCCAGTGA CCTGCTGGCA CAGAGAACCA CCTCCCTTTA CAGTGACGAG 
GAGTCTATTC TCTCCCGCTT TGATGAAGAG GACCTGGGAG ATGAGATGGC CTGTGTCCAT 
GCCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1f ( XM_006256698.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256760.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X2, mRNA.

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CloneID	ORa39597
Clone ID Related Accession (Same CDS sequence)	XM_006256698.3
Accession Version	XM_006256698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGC TTGTGCCGCT TCTGCACATT GCCCTGTTGG TGCTCTTCGT CATTATCATT 
TACGCCATCA TCGGACTCGA GCTGTTCCTC GGACGAATGC ACAAGACGTG CTACTTCCTG 
GGATCTGATA TGGAGGCAGA GGAGGACCCG TCGCCTTGTG CATCTTCTGG CTCTGGCCGT 
TCATGCACAC TGAACCAGAC TGAGTGCCGT GGGCGCTGGC CAGGACCCAA CGGTGGCATC 
ACGAACTTTG ACAATTTTTT CTTTGCCATG CTAACTGTGT TCCAGTGTAT TACCATGGAA 
GGCTGGACAG ACGTCCTCTA CTGGATGCAG GATGCCATGG GGTATGAGCT ACCTTGGGTG 
TACTTTGTGA GCCTCGTCAT CTTTGGGTCT TTCTTTGTCC TCAACCTTGT GCTTGGAGTC 
CTAAGTGGGG AGTTCTCCAA GGAAAGAGAA AAGGCAAAAG CCCGAGGTGA CTTTCAGAAG 
CTTCGGGAGA AGCAGCAGAT GGAAGAGGAC CTTCGGGGCT ACCTGGACTG GATCACACAG 
GCTGAGGAGT TGGACCTTCA TGACCCCTCA GTAGACGGCA ACTTGGCTTC TCTTGCTGAA 
GAGGGACGGG CAGGCCATCG ACCACAACTG TCAGAGCTGA CCAATAGGAG GCGCGGACGA 
CTGCGTTGGT TCAGCCACTC CACTCGCTCC ACACACTCCA CCAGCAGTCA TGCCAGCCTT 
CCAGCCAGTG ACACTGGTTC CATAACAGAC ACCCCCGGAG ATGAGGATGA AGAGGAGGGG 
ACCATGGCCA GCTGTACACT CTGCCTAAAC AAGATTATGA AAACCAAGGT CTGCCGCCAC 
TTCCGCCGAG CCAACCGTGG TCTCCGTGCA CGCTGCCGCC GGGCAGTCAA GTCCAATGCC 
TGCTACTGGG CTGTATTGCT GCTCGTCTTC CTCAACACGT TGACCATCGC TTCTGAGCAC 
CATGGGCAGC CAGTGTGGCT CACCCAGACC CAAGAATATG CCAACAAGGT TCTGCTCTGC 
CTCTTCACCG TGGAGATGCT TCTCAAACTG TACGGCCTGG GCCCCTCTGT CTACGTTGCC 
TCTTTTTTCA ACCGCTTTGA CTGTTTCGTG GTCTGTGGGG GCATCCTAGA AACGACGTTG 
GTGGAGGTGG GGGCCATGCA GCCCCTCGGC ATCTCAGTGC TCCGATGTGT GCGTCTCCTC 
AGGATCTTCA AGGTCACCAG GCACTGGGCA TCCCTCAGCA ATCTGGTGGC ATCGCTGCTC 
AACTCCATGA AGTCCATCGC CTCCTTGCTG CTTCTCCTCT TTCTCTTTAT CATCATCTTC 
TCCCTTCTTG GCATGCAGCT GTTTGGGGGC AAGTTCAACT TTGACCAGAC CCACACTAAG 
AGAAGCACCT TTGATACCTT CCCCCAAGCC CTCCTCACCG TCTTTCAGAT CCTGACTGGT 
GAGGATTGGA ATGTAGTCAT GTATGACGGT ATCATGGCCT ACGGTGGTCC CTTCTTCCCA 
GGGATGCTGG TGTGTGTTTA TTTCATCATC CTCTTCATCT GTGGCAACTA CATCCTACTG 
AACGTGTTTC TTGCCATTGC TGTGGATAAC CTAGCCAGCG GGGATGCGGG TGCTGCCAAA 
GATAAGGGCA GAGAGAAGAG CAGTGAAGGA AACCCTCCCC AAGAGAACAA AGTATTGGTG 
CCTGGTGGAG AAAATGAGGA CACAGAGGGC ACAAAAAGTG AAGGAGCAGC ACCAGGCATG 
GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG GAGGAAGAAA ATGGTGCAGG ACATGTGGAA 
CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAAGG CAGTGCCTTC 
TTCTGCCTCA GCCAAACCAA CCCGCTTCGC AAGGCCTGCC ACACTCTCAT ACACCATCAC 
GTGTTCACCA GTCTCATCCT GGTGTTCATC ATCCTCAGTA GTGTGTCCCT GGCTGCTGAG 
GACCCCATCA GAGCCCACTC CTTCCGCAAC CATATTCTGG GGTACTTCGA TTATGCCTTC 
ACTTCCATTT TCACCGTGGA GATTCTATTA AAGATGACGG TGTTTGGGGC TTTCCTGCAC 
CAAGGTTCTT TCTGCCGTAG CTGGTTCAAT CTGTTGGATC TGCTTGTGGT CAGTGTGTCC 
CTCATCTCCT TTGGCATCCA CTCCAGTGCC ATCTCAGTTG TGAAGATTCT CCGAGTCCTC 
CGAGTCCTGC GGCCTCTCCG AGCCATTAAC CGAGCCAAGG GACTCAAGCA TGTGGTGCAG 
TGTGTGTTTG TGGCCATCCG GACCATTGGA AACATCATGA TTGTCACCAC CCTCTTGCAG 
TTCATGTTCG CCTGCATTGG TGTTCAGCTG TTCAAGGGAA AATTCTACAG TTGCACTGAT 
GAAGCCAAAC ACACCCTGAA AGAATGCAAG GGCTCCTTCC TCATCTACCC TGATGGAGAT 
GTGTCACGAC CTTTGGTCCG GGAACGCCTC TGGGTCAACA GTGATTTCAA CTTTGACAAC 
GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCTACCT TTGAAGGCTG GCCTGCGCTA 
CTATACAAAG CCATAGATGC ACACGCTGAA GACGAGGGCC CTATCTACAA TTACCATGTG 
GAGATATCAG TATTCTTCAT TGTCTACATC ATCATCATCG CCTTCTTCAT GATGAACATC 
TTTGTGGGAT TTGTCATCAT CACATTCCGT GCCCAGGGAG AGCAGGAATA TCAAAACTGT 
GAACTGGACA AGAACCAGCG CCAGTGTGTG GAATATGCAC TCAAAGCTCA GCCACTCCGC 
CGATACATCC CCAAGAATCC ACATCAGTAC CGCGTGTGGG CCACAGTGAA CTCAGCCGCC 
TTTGAGTACC TCATGTTTCT GCTCATCCTC CTCAACACAG TTGCCCTGGC CATGCAGCAC 
TATGAGCAGA CGGCTCCCTT TAACTATGCC ATGGACATAC TCAACATGGT CTTCACTGGC 
CTCTTCACCG TTGAGATGGT GCTCAAAATC ATTGCCTTTA AACCCAAGCA TTACTTTGCC 
GATGCCTGGA ATACATTCGA TGCTCTTATT GTAGTGGGCA GCGTAGTCGA CATTGCCGTC 
ACAGAAGTCA ATAATGGAGG CCATCTTGGT GAGAGCTCAG AGGACAGCTC CCGCATATCT 
ATCACGTTCT TTCGCCTCTT CCGGGTCATG AGGCTGGTGA AGCTGCTCAG TAAGGGTGAG 
GGGATCCGCA CACTGCTCTG GACATTCATC AAGTCTTTCC AGGCCTTGCC CTATGTGGCA 
CTTCTTATAG CAATGATATT CTTCATATAT GCAGTCATTG GCATGCAGAT GTTTGGCAAA 
GTGGCTCTTC AGGACGGCAC ACAGATAAAT CGAAACAATA ATTTCCAGAC CTTTCCGCAG 
GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAAGAGAT AATGCTAGCC 
AGCCTTCCAG GAAATCGGTG TGACCCTGAG TCTGACTTTG GCCCAGGCGA GGAATTTACC 
TGTGGTAGCA ATTTTGCCAT TGTCTACTTT ATCAGCTTCT TCATGCTCTG TGCCTTCCTG 
ATTATAAATC TCTTTGTGGC TGTAATCATG GATAACTTTG ATTACCTAAC CAGAGATTGG 
TCTATTCTGG GACCCCACCA CCTCGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCC 
GGAGCCAAGG GCCGCATCAA GCACTTGGAT GTGGTTGCCC TGCTGAGACG CATCCAGCCT 
CCACTGGGAT TTGGAAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT GGTGGCCATG 
AATGTTCCCC TCAACTCAGA CGGAACAGTG ACATTCAACG CGACACTCTT TGCCCTGGTG 
CGGACATCCC TGAGGATCAA GACAGAAGGG AACCTGGATC AAGCCAACCA GGAGCTTCGG 
ATGGTCATCA AAAAGATCTG GAAGCGGATA AAGCAGAAAT TGTTAGATGA GGTCATCCCC 
CCTCCAGATG AGGAGGAGGT CACTGTGGGA AAATTCTATG CCACATTTCT CATCCAAGAT 
TATTTCCGAA AATTCCGGAG AAGGAAAGAA AAGGGGCTAC TAGGAGCAGA TGCCCCAACA 
AGCACATCCT CTGTCCTCCA GGCTGGTCTA AGGAGCCTAC AGGACTTGGG TCCTGAGATC 
CGACAGGCCC TCACCTATGA CACTGAGGAA GAAGAAGAAG AAGAAGAGGC CGCAGGTCAG 
GAAGCTGAGG AGGAGGAAGC TGAGAACAAC CCAGAAACAT ACAAAGACTC CATAGACTCC 
CAGCCCCAAG CTCAATGGAA CTCGAGGATT TCGGTGTCTC TACCAGTTAA GGAGAAACTC 
CCAGATTCTC TTTCAACTGG GCCTAGTGAT GATGATGGAG TGGCTCCCAA CTCCAGGCAA 
CCCAGTGGGC TACAGGCTGG ATCCCAACCC CACAGGAGAA GCTCTGGGGT TTTCATGTTC 
ACTATCCCAG AAGAAGGAAG TACTCAGCTC AAGGGAGTTC AAGGGCAAGA CAATCAGAAT 
GAGGAACAGG AAGTCCCTGA CTGGACCCCC AACCTGGATG AGCAGGCAGG GATGCCCTCC 
AACCCAGTCC TTTTACCACC TCACTGGTCC CAGCAACATG TAAATGGGCA CCATGTACCA 
CGCCGACGTT TGCTACCCCC CACGCCTGCA GGTCGGAAGC CCTCCTTCAC CATCCAGTGT 
CTGCAACGCC AGGGCAGTTG TGAGGATTTG CCCATCCCAG GCACCTACCA TCGTGGACGG 
ACCTCAGGAC CAGGCAGGGC TCAGGGTTCC TGGGCAGCCC CTCCTCAGAA GGGTCGACTG 
CTCTATGCCC CCCTGTTGCT GGTGGAGGAA TCTACAGTGG GTGAAGGATA CCTTGGCAAG 
CTCGGCGGCC CACTGCGTAC CTTCACTTGT CTGCAAGTGC CTGGAGCTCC TTCAGATCCC 
AGTCACCGCA AGAGGGGCAG TGCTGACAGT TTGGTGGAGG CTGTGCTTAT CTCCGAAGGC 
CTAGGCCTCT TTGCCCAAGA CCCACGATTT GTGGCCCTGG CCAAGCAAGA GATTGCAGAT 
GCATGTCACC TGACCCTGGA TGAGATGGAC AGTGCTGCCA GTGACCTGCT GGCACAGAGA 
ACCACCTCCC TTTACAGTGA CGAGGAGTCT ATTCTCTCCC GCTTTGATGA AGAGGACCTG 
GGAGATGAGA TGGCCTGTGT CCATGCCCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256760.1
CDS	100..5352
Translation	MKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRSCTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSITDTPGDEDEEEGTMASCTLCLNKIMKTKVCRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGAAKDKGREKSSEGNPPQENKVLVPGGENEDTEGTKSEGAAPGMEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHVFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHQGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAHAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLRIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGADAPTSTSSVLQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAAGQEAEEEEAENNPETYKDSIDSQPQAQWNSRISVSLPVKEKLPDSLSTGPSDDDGVAPNSRQPSGLQAGSQPHRRSSGVFMFTIPEEGSTQLKGVQGQDNQNEEQEVPDWTPNLDEQAGMPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPGRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAPSDPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_006256698.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256698.3

ORF Insert Sequence:

ATGAAGGCGC TTGTGCCGCT TCTGCACATT GCCCTGTTGG TGCTCTTCGT CATTATCATT 
TACGCCATCA TCGGACTCGA GCTGTTCCTC GGACGAATGC ACAAGACGTG CTACTTCCTG 
GGATCTGATA TGGAGGCAGA GGAGGACCCG TCGCCTTGTG CATCTTCTGG CTCTGGCCGT 
TCATGCACAC TGAACCAGAC TGAGTGCCGT GGGCGCTGGC CAGGACCCAA CGGTGGCATC 
ACGAACTTTG ACAATTTTTT CTTTGCCATG CTAACTGTGT TCCAGTGTAT TACCATGGAA 
GGCTGGACAG ACGTCCTCTA CTGGATGCAG GATGCCATGG GGTATGAGCT ACCTTGGGTG 
TACTTTGTGA GCCTCGTCAT CTTTGGGTCT TTCTTTGTCC TCAACCTTGT GCTTGGAGTC 
CTAAGTGGGG AGTTCTCCAA GGAAAGAGAA AAGGCAAAAG CCCGAGGTGA CTTTCAGAAG 
CTTCGGGAGA AGCAGCAGAT GGAAGAGGAC CTTCGGGGCT ACCTGGACTG GATCACACAG 
GCTGAGGAGT TGGACCTTCA TGACCCCTCA GTAGACGGCA ACTTGGCTTC TCTTGCTGAA 
GAGGGACGGG CAGGCCATCG ACCACAACTG TCAGAGCTGA CCAATAGGAG GCGCGGACGA 
CTGCGTTGGT TCAGCCACTC CACTCGCTCC ACACACTCCA CCAGCAGTCA TGCCAGCCTT 
CCAGCCAGTG ACACTGGTTC CATAACAGAC ACCCCCGGAG ATGAGGATGA AGAGGAGGGG 
ACCATGGCCA GCTGTACACT CTGCCTAAAC AAGATTATGA AAACCAAGGT CTGCCGCCAC 
TTCCGCCGAG CCAACCGTGG TCTCCGTGCA CGCTGCCGCC GGGCAGTCAA GTCCAATGCC 
TGCTACTGGG CTGTATTGCT GCTCGTCTTC CTCAACACGT TGACCATCGC TTCTGAGCAC 
CATGGGCAGC CAGTGTGGCT CACCCAGACC CAAGAATATG CCAACAAGGT TCTGCTCTGC 
CTCTTCACCG TGGAGATGCT TCTCAAACTG TACGGCCTGG GCCCCTCTGT CTACGTTGCC 
TCTTTTTTCA ACCGCTTTGA CTGTTTCGTG GTCTGTGGGG GCATCCTAGA AACGACGTTG 
GTGGAGGTGG GGGCCATGCA GCCCCTCGGC ATCTCAGTGC TCCGATGTGT GCGTCTCCTC 
AGGATCTTCA AGGTCACCAG GCACTGGGCA TCCCTCAGCA ATCTGGTGGC ATCGCTGCTC 
AACTCCATGA AGTCCATCGC CTCCTTGCTG CTTCTCCTCT TTCTCTTTAT CATCATCTTC 
TCCCTTCTTG GCATGCAGCT GTTTGGGGGC AAGTTCAACT TTGACCAGAC CCACACTAAG 
AGAAGCACCT TTGATACCTT CCCCCAAGCC CTCCTCACCG TCTTTCAGAT CCTGACTGGT 
GAGGATTGGA ATGTAGTCAT GTATGACGGT ATCATGGCCT ACGGTGGTCC CTTCTTCCCA 
GGGATGCTGG TGTGTGTTTA TTTCATCATC CTCTTCATCT GTGGCAACTA CATCCTACTG 
AACGTGTTTC TTGCCATTGC TGTGGATAAC CTAGCCAGCG GGGATGCGGG TGCTGCCAAA 
GATAAGGGCA GAGAGAAGAG CAGTGAAGGA AACCCTCCCC AAGAGAACAA AGTATTGGTG 
CCTGGTGGAG AAAATGAGGA CACAGAGGGC ACAAAAAGTG AAGGAGCAGC ACCAGGCATG 
GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG GAGGAAGAAA ATGGTGCAGG ACATGTGGAA 
CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAAGG CAGTGCCTTC 
TTCTGCCTCA GCCAAACCAA CCCGCTTCGC AAGGCCTGCC ACACTCTCAT ACACCATCAC 
GTGTTCACCA GTCTCATCCT GGTGTTCATC ATCCTCAGTA GTGTGTCCCT GGCTGCTGAG 
GACCCCATCA GAGCCCACTC CTTCCGCAAC CATATTCTGG GGTACTTCGA TTATGCCTTC 
ACTTCCATTT TCACCGTGGA GATTCTATTA AAGATGACGG TGTTTGGGGC TTTCCTGCAC 
CAAGGTTCTT TCTGCCGTAG CTGGTTCAAT CTGTTGGATC TGCTTGTGGT CAGTGTGTCC 
CTCATCTCCT TTGGCATCCA CTCCAGTGCC ATCTCAGTTG TGAAGATTCT CCGAGTCCTC 
CGAGTCCTGC GGCCTCTCCG AGCCATTAAC CGAGCCAAGG GACTCAAGCA TGTGGTGCAG 
TGTGTGTTTG TGGCCATCCG GACCATTGGA AACATCATGA TTGTCACCAC CCTCTTGCAG 
TTCATGTTCG CCTGCATTGG TGTTCAGCTG TTCAAGGGAA AATTCTACAG TTGCACTGAT 
GAAGCCAAAC ACACCCTGAA AGAATGCAAG GGCTCCTTCC TCATCTACCC TGATGGAGAT 
GTGTCACGAC CTTTGGTCCG GGAACGCCTC TGGGTCAACA GTGATTTCAA CTTTGACAAC 
GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCTACCT TTGAAGGCTG GCCTGCGCTA 
CTATACAAAG CCATAGATGC ACACGCTGAA GACGAGGGCC CTATCTACAA TTACCATGTG 
GAGATATCAG TATTCTTCAT TGTCTACATC ATCATCATCG CCTTCTTCAT GATGAACATC 
TTTGTGGGAT TTGTCATCAT CACATTCCGT GCCCAGGGAG AGCAGGAATA TCAAAACTGT 
GAACTGGACA AGAACCAGCG CCAGTGTGTG GAATATGCAC TCAAAGCTCA GCCACTCCGC 
CGATACATCC CCAAGAATCC ACATCAGTAC CGCGTGTGGG CCACAGTGAA CTCAGCCGCC 
TTTGAGTACC TCATGTTTCT GCTCATCCTC CTCAACACAG TTGCCCTGGC CATGCAGCAC 
TATGAGCAGA CGGCTCCCTT TAACTATGCC ATGGACATAC TCAACATGGT CTTCACTGGC 
CTCTTCACCG TTGAGATGGT GCTCAAAATC ATTGCCTTTA AACCCAAGCA TTACTTTGCC 
GATGCCTGGA ATACATTCGA TGCTCTTATT GTAGTGGGCA GCGTAGTCGA CATTGCCGTC 
ACAGAAGTCA ATAATGGAGG CCATCTTGGT GAGAGCTCAG AGGACAGCTC CCGCATATCT 
ATCACGTTCT TTCGCCTCTT CCGGGTCATG AGGCTGGTGA AGCTGCTCAG TAAGGGTGAG 
GGGATCCGCA CACTGCTCTG GACATTCATC AAGTCTTTCC AGGCCTTGCC CTATGTGGCA 
CTTCTTATAG CAATGATATT CTTCATATAT GCAGTCATTG GCATGCAGAT GTTTGGCAAA 
GTGGCTCTTC AGGACGGCAC ACAGATAAAT CGAAACAATA ATTTCCAGAC CTTTCCGCAG 
GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAAGAGAT AATGCTAGCC 
AGCCTTCCAG GAAATCGGTG TGACCCTGAG TCTGACTTTG GCCCAGGCGA GGAATTTACC 
TGTGGTAGCA ATTTTGCCAT TGTCTACTTT ATCAGCTTCT TCATGCTCTG TGCCTTCCTG 
ATTATAAATC TCTTTGTGGC TGTAATCATG GATAACTTTG ATTACCTAAC CAGAGATTGG 
TCTATTCTGG GACCCCACCA CCTCGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCC 
GGAGCCAAGG GCCGCATCAA GCACTTGGAT GTGGTTGCCC TGCTGAGACG CATCCAGCCT 
CCACTGGGAT TTGGAAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT GGTGGCCATG 
AATGTTCCCC TCAACTCAGA CGGAACAGTG ACATTCAACG CGACACTCTT TGCCCTGGTG 
CGGACATCCC TGAGGATCAA GACAGAAGGG AACCTGGATC AAGCCAACCA GGAGCTTCGG 
ATGGTCATCA AAAAGATCTG GAAGCGGATA AAGCAGAAAT TGTTAGATGA GGTCATCCCC 
CCTCCAGATG AGGAGGAGGT CACTGTGGGA AAATTCTATG CCACATTTCT CATCCAAGAT 
TATTTCCGAA AATTCCGGAG AAGGAAAGAA AAGGGGCTAC TAGGAGCAGA TGCCCCAACA 
AGCACATCCT CTGTCCTCCA GGCTGGTCTA AGGAGCCTAC AGGACTTGGG TCCTGAGATC 
CGACAGGCCC TCACCTATGA CACTGAGGAA GAAGAAGAAG AAGAAGAGGC CGCAGGTCAG 
GAAGCTGAGG AGGAGGAAGC TGAGAACAAC CCAGAAACAT ACAAAGACTC CATAGACTCC 
CAGCCCCAAG CTCAATGGAA CTCGAGGATT TCGGTGTCTC TACCAGTTAA GGAGAAACTC 
CCAGATTCTC TTTCAACTGG GCCTAGTGAT GATGATGGAG TGGCTCCCAA CTCCAGGCAA 
CCCAGTGGGC TACAGGCTGG ATCCCAACCC CACAGGAGAA GCTCTGGGGT TTTCATGTTC 
ACTATCCCAG AAGAAGGAAG TACTCAGCTC AAGGGAGTTC AAGGGCAAGA CAATCAGAAT 
GAGGAACAGG AAGTCCCTGA CTGGACCCCC AACCTGGATG AGCAGGCAGG GATGCCCTCC 
AACCCAGTCC TTTTACCACC TCACTGGTCC CAGCAACATG TAAATGGGCA CCATGTACCA 
CGCCGACGTT TGCTACCCCC CACGCCTGCA GGTCGGAAGC CCTCCTTCAC CATCCAGTGT 
CTGCAACGCC AGGGCAGTTG TGAGGATTTG CCCATCCCAG GCACCTACCA TCGTGGACGG 
ACCTCAGGAC CAGGCAGGGC TCAGGGTTCC TGGGCAGCCC CTCCTCAGAA GGGTCGACTG 
CTCTATGCCC CCCTGTTGCT GGTGGAGGAA TCTACAGTGG GTGAAGGATA CCTTGGCAAG 
CTCGGCGGCC CACTGCGTAC CTTCACTTGT CTGCAAGTGC CTGGAGCTCC TTCAGATCCC 
AGTCACCGCA AGAGGGGCAG TGCTGACAGT TTGGTGGAGG CTGTGCTTAT CTCCGAAGGC 
CTAGGCCTCT TTGCCCAAGA CCCACGATTT GTGGCCCTGG CCAAGCAAGA GATTGCAGAT 
GCATGTCACC TGACCCTGGA TGAGATGGAC AGTGCTGCCA GTGACCTGCT GGCACAGAGA 
ACCACCTCCC TTTACAGTGA CGAGGAGTCT ATTCTCTCCC GCTTTGATGA AGAGGACCTG 
GGAGATGAGA TGGCCTGTGT CCATGCCCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1f ( NM_053701.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446153.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), mRNA.

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CloneID	ORa10353
Clone ID Related Accession (Same CDS sequence)	NM_053701.1
Accession Version	NM_053701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-28
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF365975.1. On Jan 12, 2005 this sequence version replaced XM_346865.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF365975.1, DQ393415.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGGAAT CTGAAGTCGG GAAAGACACC ACCCCAGAAC CCAGTCCAGC CAATGGGACC 
GGTCCTGGCC CTGAGTGGGG GCTCTGTCCT GGGCCTCCAA CCGTGGGGAC TGATACCAGT 
GGGGCATCGG GCCTGGGGAC CCCAAGACGG AGGACTCAGC ACAACAAGCA CAAGACCGTA 
GTGGTGGCCA GTGCGCAGAG ATCACCTCGA GCACTCTTCT GCCTCACCTT AACCAATCCC 
ATTCGTCGGT CCTGCATCAG CATTGTGGAG TGGAAGCCAT TTGATATTCT CATCCTCCTG 
ACCATCTTTG CCAACTGTGT GGCATTGGGG GTATATATCC CCTTCCCTGA GGACGACTCC 
AACACTGCTA CCCACAACTT GGAACAGGTA GAATATGTGT TCCTGGTGAT TTTCACCGTG 
AAGACAGTGC TCAAGATCGT GGCCTATGGG CTGGTGCTCC ATCCCAGCGC CTACATTCGC 
AATGGTTGGA ACCTACTCGA CTTCATCATC GTTGTAGTCG GGCTGTTCAG CGTGTTGCTG 
GAGCAGGGAC CTGGGCGGCC AGGAGATGCC CCGCATACTG GAGGAAAGCC GGGAGGCTTT 
GATGTAAAGG CACTGCGGGC ATTTAGGGTG CTACGACCAC TAAGGCTAGT GTCTGGGGTC 
CCGAGTCTGC ACATAGTGCT CAATTCCATC ATGAAGGCGC TTGTGCCGCT TCTGCACATT 
GCCCTGTTGG TGCTCTTCGT CATTATCATT TACGCCATCA TCGGACTCGA GCTGTTCCTC 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAGGCAGA GGAGGACCCG 
TCGCCTTGTG CATCTTCTGG CTCTGGCCGT TCATGCACAC TGAACCAGAC TGAGTGCCGT 
GGGCGCTGGC CAGGACCCAA CGGTGGCATC ACGAACTTTG ACAATTTTTT CTTTGCCATG 
CTAACTGTGT TCCAGTGTAT TACCATGGAA GGCTGGACAG ACGTCCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT ACCTTGGGTG TACTTTGTGA GCCTCGTCAT CTTTGGGTCT 
TTCTTTGTCC TCAACCTTGT GCTTGGAGTC CTAAGTGGGG AGTTCTCCAA GGAAAGAGAA 
AAGGCAAAAG CCCGAGGTGA CTTTCAGAAG CTTCGGGAGA AGCAGCAGAT GGAAGAGGAC 
CTTCGGGGCT ACCTGGACTG GATCACACAG GCTGAGGAGT TGGACCTTCA TGACCCCTCA 
GTAGACGGCA ACTTGGCTTC TCTTGCTGAA GAGGGACGGG CAGGCCATCG ACCACAACTG 
TCAGAGCTGA CCAATAGGAG GCGCGGACGA CTGCGTTGGT TCAGCCACTC CACTCGCTCC 
ACACACTCCA CCAGCAGTCA TGCCAGCCTT CCAGCCAGTG ACACTGGTTC CATAACAGAC 
ACCCCCGGAG ATGAGGATGA AGAGGAGGGG ACCATGGCCA GCTGTACACT CTGCCTAAAC 
AAGATTATGA AAACCAAGGT CTGCCGCCAC TTCCGCCGAG CCAACCGTGG TCTCCGTGCA 
CGCTGCCGCC GGGCAGTCAA GTCCAATGCC TGCTACTGGG CTGTATTGCT GCTCGTCTTC 
CTCAACACGT TGACCATCGC TTCTGAGCAC CATGGGCAGC CAGTGTGGCT CACCCAGACC 
CAAGAATATG CCAACAAGGT TCTGCTCTGC CTCTTCACCG TGGAGATGCT TCTCAAACTG 
TACGGGCTGG GCCCCTCTGT CTACGTTGCC TCTTTTTTCA ACCGCTTTGA CTGTTTCGTG 
GTCTGTGGGG GCATCCTAGA AACGACGTTG GTGGAGGTGG GGGCCATGCA GCCCCTCGGC 
ATCTCAGTGC TCCGATGTGT GCGTCTCCTC AGGATCTTCA AGGTCACCAG GCACTGGGCA 
TCCCTCAGCA ATCTGGTGGC ATCGCTGCTC AACTCCATGA AGTCCATCGC CTCCTTGCTG 
CTTCTCCTCT TTCTCTTTAT CATCATCTTC TCCCTTCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACTAAG AGAAGCACCT TTGATACCTT CCCCCAAGCC 
CTCCTCACCG TCTTTCAGAT CCTGACTGGT GAGGATTGGA ATGTAGTCAT GTATGACGGT 
ATCATGGCCT ACGGTGGTCC CTTCTTCCCA GGGATGCTGG TGTGTGTTTA TTTCATCATC 
CTCTTCATCT GTGGCAACTA CATCCTACTG AACGTGTTTC TTGCCATTGC TGTGGATAAC 
CTAGCCAGCG GGGATGCGGG TGCTGCCAAA GATAAGGGCA GAGAGAAGAG CAGTGAAGGA 
AACCCTCCCC AAGAGAACAA AGTATTGGTG CCTGGTGGAG AAAATGAGGA CACAGAGGGC 
ACAAAAAGTG AAGGAGCAGC ACCAGGCATG GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG 
GAGGAAGAAA ATGGTGCAGG ACATGTGGAA CTCCTGCAGG AAGTTGTACC CAAGGAGAAG 
GTGGTACCCA TCCCTGAAGG CAGTGCCTTC TTCTGCCTCA GCCAAACCAA CCCGCTTCGC 
AAGGCCTGCC ACACTCTCAT ACACCATCAC GTGTTCACCA GTCTCATCCT GGTGTTCATC 
ATCCTCAGTA GTGTGTCCCT GGCTGCTGAG GACCCCATCA GAGCCCACTC TTTCCGCAAC 
CATATTCTGG GGTACTTCGA TTATGCCTTC ACTTCCATTT TCACCGTGGA GATTCTATTA 
AAGATGACGG TGTTTGGGGC TTTCCTGCAC CAAGGTTCTT TCTGCCGTAG CTGGTTCAAT 
CTGTTGGATC TGCTTGTGGT CAGTGTGTCC CTCATCTCCT TTGGCATCCA CTCCAGTGCC 
ATCTCAGTTG TGAAGATTCT CCGAGTCCTC CGAGTCCTGC GGCCTCTCCG AGCCATTAAC 
CGAGCCAAGG GACTCAAGCA TGTGGTGCAG TGTGTGTTTG TGGCCATCCG GACCATTGGA 
AACATCATGA TTGTCACCAC CCTCTTGCAG TTCATGTTCG CCTGCATTGG TGTTCAGCTG 
TTCAAGGGAA AATTCTACAG TTGCACTGAT GAAGCCAAAC ACACCCTGAA AGAATGCAAG 
GGCTCCTTCC TCATCTACCC TGATGGAGAT GTGTCACGAC CTTTGGTCCG GGAACGCCTC 
TGGGTCAACA GTGATTTCAA CTTTGACAAC GTCCTTTCAG CCATGATGGC CCTGTTCACT 
GTCTCTACCT TTGAAGGCTG GCCTGCGCTA CTATACAAAG CCATAGATGC ACACGCTGAA 
GACGAGGGCC CTATCTACAA TTACCATGTG GAGATATCAG TATTCTTCAT TGTCTACATC 
ATCATCATCG CCTTCTTCAT GATGAACATC TTTGTGGGAT TTGTCATCAT CACATTCCGT 
GCCCAGGGAG AGCAGGAATA TCAAAACTGT GAACTGGACA AGAACCAGCG CCAGTGTGTG 
GAATATGCAC TCAAAGCTCA GCCACTCCGC CGATACATCC CCAAGAATCC ACATCAGTAC 
CGCGTGTGGG CCACAGTGAA CTCAGCCGCC TTTGAGTACC TCATGTTTCT GCTCATCCTC 
CTCAACACAG TTGCCCTGGC CATGCAGCAC TATGAGCAGA CGGCTCCCTT TAACTATGCC 
ATGGACATAC TCAACATGGT CTTCACTGGC CTCTTCACCG TTGAGATGGT GCTCAAAATC 
ATTGCCTTTA AACCCAAGCA TTACTTTGCC GATGCCTGGA ATACATTCGA TGCTCTTATT 
GTAGTGGGCA GCGTAGTCGA CATTGCCGTC ACAGAAGTCA ATAATGGAGG CCATCTTGGT 
GAGAGCTCAG AGGACAGCTC CCGCATATCT ATCACGTTCT TTCGCCTCTT CCGGGTCATG 
AGGCTGGTGA AGCTGCTCAG TAAGGGTGAG GGGATCCGCA CACTGCTCTG GACATTCATC 
AAGTCTTTCC AGGCCTTGCC CCATGTGGCA CTTCTTATAG CAATGATATT CTTCATATAT 
GCAGTCATTG GCATGCAGAT GTTTGGCAAA GTGGCTCTTC AGGACGGCAC ACAGATAAAT 
CGAAACAATA ATTTCCAGAC CTTTCCGCAG GCTGTGCTGC TTCTGTTCAG GTGTGCCACT 
GGTGAGGCAT GGCAAGAGAT AATGCTAGCC AGCCTTCCAG GAAATCGGTG TGACCCTGAG 
TCTGACTTTG GCCCAGGCGA GGAATTTACC TGTGGTAGCA ATTTTGCCAT TGTCTACTTT 
ATCAGCTTCT TCATGCTCTG TGCCTTCCTG ATTATAAATC TCTTTGTGGC TGTAATCATG 
GATAACTTTG ATTACCTAAC CAGAGATTGG TCTATTCTGG GACCCCACCA CCTCGATGAA 
TTCAAGAGGA TCTGGTCTGA ATATGACCCC GGAGCCAAGG GCCGCATCAA GCACTTGGAT 
GTGGTTGCCC TGCTGAGACG CATCCAGCCT CCACTGGGAT TTGGAAAGCT GTGCCCACAC 
CGAGTGGCCT GCAAGAGACT GGTGGCCATG AATGTTCCCC TCAACTCAGA CGGAACAGTG 
ACATTCAACG CGACACTCTT TGCCCTGGTG CGGACATCCC TGAGGATCAA GACAGAAGGG 
AACCTGGATC AAGCCAACCA GGAGCTTCGG ATGGTCATCA AAAAGATCTG GAAGCGGATA 
AAGCAGAAAT TGTTAGATGA GGTCATCCCC CCTCCAGATG AGGAGGAGGT CACTGTGGGA 
AAATTCTATG CCACATTTCT CATCCAAGAT TATTTCCGAA AATTCCGGAG AAGGAAAGAA 
AAGGGGCTAC TAGGAGCAGA TGCCCCAACA AGCACATCCT CTGTCCTCCA GGCTGGTCTA 
AGGAGCCTAC AGGACTTGGG TCCTGAGATC CGACAGGCCC TCACCTATGC CCTTGAGGAA 
GAAGAAGAAG AAGAAGAGGC CCGCCAGGTC AAGGAAGCTG AGGAGGAGGA AGCTGAGACC 
ACCCCAGAAA CATACAAAGA CTCCATAGAC TCCCAGCCCC AAGCTCAATG GAACTCGAGG 
ATTTCGGTGT CTCTACCCAA GTTAAGAGAA ACTCCAGATT CTCTTTCAAC TGGGCCTAGT 
GATGATGATG GAGTGGCTCC CAACTCCAGG CAACCCAGTG GGCTACAGGC TGGATCCCAA 
CCCCACAGGA GAGGCTCTGG GGTTTTCATG TTCACTATCC CAGAAGAAGG AAGTACTCAG 
CTCAAGGGAG TTCAAGGGCA AGACAATCAG AATGAGGAAC AGGAAGTCCC TGACTGGACC 
CCCAACCTGG ATGAGCAGGC AGGGATGCCC TCCAACCCAG TCCTTTTACC ACCTCACTGG 
TCCCAGCAAC ATGTAAATGG GCACCATGTA CCACGCCGAC GTTTGCTACC CCCCACGCCT 
GCAGGTCGGA AGCCCTCCTT CACCATCCAG TGTCTGCAAC GCCAGGGCAG TTGTGAGGAT 
TTGCCCATCC CAGGCACCTA CCATCGTGGA CGGACCTCAG GACCAGGCAG GGCTCAGGGT 
TCCTGGGCAG CCCCTCCTCA GAAGGGTCGA CTGCTCTATG CCCCCCTGTT GCTGGTGGAG 
GAATCTACAG TGGGTGAAGG ATACCTTGGC AAGCTCGGCG GCCCACTGCG TACCTTCACT 
TGTCTGCAAG TGCCTGGAGC TCCTTCAGAT CCCAGTCACC GCAAGAGGGG CAGTGCTGAC 
AGTTTGGTGG AGGCTGTGCT TATCTCCGAA GGCCTAGGCC TCTTTGCCCA AGACCCACGA 
TTTGTGGCCC TGGCCAAGCA AGAGATTGCA GATGCATGTC ACCTGACCCT GGATGAGATG 
GACAGTGCTG CCAGTGACCT GCTGGCACAG AGAACCACCT CCCTCTACAG TGACGAGGAG 
TCTATTCTCT CCCGCTTTGA TGAAGAGGAC CTGGGAGACG AGATGGCCTG CGTCCATGCC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446153.1
CDS	30..5975
Translation	MSESEVGKDTTPEPSPANGTGPGPEWGLCPGPPTVGTDTSGASGLGTPRRRTQHNKHKTVVVASAQRSPRALFCLTLTNPIRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTATHNLEQVEYVFLVIFTVKTVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRSCTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSITDTPGDEDEEEGTMASCTLCLNKIMKTKVCRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGAAKDKGREKSSEGNPPQENKVLVPGGENEDTEGTKSEGAAPGMEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHVFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHQGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAHAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPHVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLRIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGADAPTSTSSVLQAGLRSLQDLGPEIRQALTYALEEEEEEEEARQVKEAEEEEAETTPETYKDSIDSQPQAQWNSRISVSLPKLRETPDSLSTGPSDDDGVAPNSRQPSGLQAGSQPHRRGSGVFMFTIPEEGSTQLKGVQGQDNQNEEQEVPDWTPNLDEQAGMPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPGRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAPSDPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_053701.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053701.1

ORF Insert Sequence:

ATGTCGGAAT CTGAAGTCGG GAAAGACACC ACCCCAGAAC CCAGTCCAGC CAATGGGACC 
GGTCCTGGCC CTGAGTGGGG GCTCTGTCCT GGGCCTCCAA CCGTGGGGAC TGATACCAGT 
GGGGCATCGG GCCTGGGGAC CCCAAGACGG AGGACTCAGC ACAACAAGCA CAAGACCGTA 
GTGGTGGCCA GTGCGCAGAG ATCACCTCGA GCACTCTTCT GCCTCACCTT AACCAATCCC 
ATTCGTCGGT CCTGCATCAG CATTGTGGAG TGGAAGCCAT TTGATATTCT CATCCTCCTG 
ACCATCTTTG CCAACTGTGT GGCATTGGGG GTATATATCC CCTTCCCTGA GGACGACTCC 
AACACTGCTA CCCACAACTT GGAACAGGTA GAATATGTGT TCCTGGTGAT TTTCACCGTG 
AAGACAGTGC TCAAGATCGT GGCCTATGGG CTGGTGCTCC ATCCCAGCGC CTACATTCGC 
AATGGTTGGA ACCTACTCGA CTTCATCATC GTTGTAGTCG GGCTGTTCAG CGTGTTGCTG 
GAGCAGGGAC CTGGGCGGCC AGGAGATGCC CCGCATACTG GAGGAAAGCC GGGAGGCTTT 
GATGTAAAGG CACTGCGGGC ATTTAGGGTG CTACGACCAC TAAGGCTAGT GTCTGGGGTC 
CCGAGTCTGC ACATAGTGCT CAATTCCATC ATGAAGGCGC TTGTGCCGCT TCTGCACATT 
GCCCTGTTGG TGCTCTTCGT CATTATCATT TACGCCATCA TCGGACTCGA GCTGTTCCTC 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAGGCAGA GGAGGACCCG 
TCGCCTTGTG CATCTTCTGG CTCTGGCCGT TCATGCACAC TGAACCAGAC TGAGTGCCGT 
GGGCGCTGGC CAGGACCCAA CGGTGGCATC ACGAACTTTG ACAATTTTTT CTTTGCCATG 
CTAACTGTGT TCCAGTGTAT TACCATGGAA GGCTGGACAG ACGTCCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT ACCTTGGGTG TACTTTGTGA GCCTCGTCAT CTTTGGGTCT 
TTCTTTGTCC TCAACCTTGT GCTTGGAGTC CTAAGTGGGG AGTTCTCCAA GGAAAGAGAA 
AAGGCAAAAG CCCGAGGTGA CTTTCAGAAG CTTCGGGAGA AGCAGCAGAT GGAAGAGGAC 
CTTCGGGGCT ACCTGGACTG GATCACACAG GCTGAGGAGT TGGACCTTCA TGACCCCTCA 
GTAGACGGCA ACTTGGCTTC TCTTGCTGAA GAGGGACGGG CAGGCCATCG ACCACAACTG 
TCAGAGCTGA CCAATAGGAG GCGCGGACGA CTGCGTTGGT TCAGCCACTC CACTCGCTCC 
ACACACTCCA CCAGCAGTCA TGCCAGCCTT CCAGCCAGTG ACACTGGTTC CATAACAGAC 
ACCCCCGGAG ATGAGGATGA AGAGGAGGGG ACCATGGCCA GCTGTACACT CTGCCTAAAC 
AAGATTATGA AAACCAAGGT CTGCCGCCAC TTCCGCCGAG CCAACCGTGG TCTCCGTGCA 
CGCTGCCGCC GGGCAGTCAA GTCCAATGCC TGCTACTGGG CTGTATTGCT GCTCGTCTTC 
CTCAACACGT TGACCATCGC TTCTGAGCAC CATGGGCAGC CAGTGTGGCT CACCCAGACC 
CAAGAATATG CCAACAAGGT TCTGCTCTGC CTCTTCACCG TGGAGATGCT TCTCAAACTG 
TACGGGCTGG GCCCCTCTGT CTACGTTGCC TCTTTTTTCA ACCGCTTTGA CTGTTTCGTG 
GTCTGTGGGG GCATCCTAGA AACGACGTTG GTGGAGGTGG GGGCCATGCA GCCCCTCGGC 
ATCTCAGTGC TCCGATGTGT GCGTCTCCTC AGGATCTTCA AGGTCACCAG GCACTGGGCA 
TCCCTCAGCA ATCTGGTGGC ATCGCTGCTC AACTCCATGA AGTCCATCGC CTCCTTGCTG 
CTTCTCCTCT TTCTCTTTAT CATCATCTTC TCCCTTCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACTAAG AGAAGCACCT TTGATACCTT CCCCCAAGCC 
CTCCTCACCG TCTTTCAGAT CCTGACTGGT GAGGATTGGA ATGTAGTCAT GTATGACGGT 
ATCATGGCCT ACGGTGGTCC CTTCTTCCCA GGGATGCTGG TGTGTGTTTA TTTCATCATC 
CTCTTCATCT GTGGCAACTA CATCCTACTG AACGTGTTTC TTGCCATTGC TGTGGATAAC 
CTAGCCAGCG GGGATGCGGG TGCTGCCAAA GATAAGGGCA GAGAGAAGAG CAGTGAAGGA 
AACCCTCCCC AAGAGAACAA AGTATTGGTG CCTGGTGGAG AAAATGAGGA CACAGAGGGC 
ACAAAAAGTG AAGGAGCAGC ACCAGGCATG GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG 
GAGGAAGAAA ATGGTGCAGG ACATGTGGAA CTCCTGCAGG AAGTTGTACC CAAGGAGAAG 
GTGGTACCCA TCCCTGAAGG CAGTGCCTTC TTCTGCCTCA GCCAAACCAA CCCGCTTCGC 
AAGGCCTGCC ACACTCTCAT ACACCATCAC GTGTTCACCA GTCTCATCCT GGTGTTCATC 
ATCCTCAGTA GTGTGTCCCT GGCTGCTGAG GACCCCATCA GAGCCCACTC TTTCCGCAAC 
CATATTCTGG GGTACTTCGA TTATGCCTTC ACTTCCATTT TCACCGTGGA GATTCTATTA 
AAGATGACGG TGTTTGGGGC TTTCCTGCAC CAAGGTTCTT TCTGCCGTAG CTGGTTCAAT 
CTGTTGGATC TGCTTGTGGT CAGTGTGTCC CTCATCTCCT TTGGCATCCA CTCCAGTGCC 
ATCTCAGTTG TGAAGATTCT CCGAGTCCTC CGAGTCCTGC GGCCTCTCCG AGCCATTAAC 
CGAGCCAAGG GACTCAAGCA TGTGGTGCAG TGTGTGTTTG TGGCCATCCG GACCATTGGA 
AACATCATGA TTGTCACCAC CCTCTTGCAG TTCATGTTCG CCTGCATTGG TGTTCAGCTG 
TTCAAGGGAA AATTCTACAG TTGCACTGAT GAAGCCAAAC ACACCCTGAA AGAATGCAAG 
GGCTCCTTCC TCATCTACCC TGATGGAGAT GTGTCACGAC CTTTGGTCCG GGAACGCCTC 
TGGGTCAACA GTGATTTCAA CTTTGACAAC GTCCTTTCAG CCATGATGGC CCTGTTCACT 
GTCTCTACCT TTGAAGGCTG GCCTGCGCTA CTATACAAAG CCATAGATGC ACACGCTGAA 
GACGAGGGCC CTATCTACAA TTACCATGTG GAGATATCAG TATTCTTCAT TGTCTACATC 
ATCATCATCG CCTTCTTCAT GATGAACATC TTTGTGGGAT TTGTCATCAT CACATTCCGT 
GCCCAGGGAG AGCAGGAATA TCAAAACTGT GAACTGGACA AGAACCAGCG CCAGTGTGTG 
GAATATGCAC TCAAAGCTCA GCCACTCCGC CGATACATCC CCAAGAATCC ACATCAGTAC 
CGCGTGTGGG CCACAGTGAA CTCAGCCGCC TTTGAGTACC TCATGTTTCT GCTCATCCTC 
CTCAACACAG TTGCCCTGGC CATGCAGCAC TATGAGCAGA CGGCTCCCTT TAACTATGCC 
ATGGACATAC TCAACATGGT CTTCACTGGC CTCTTCACCG TTGAGATGGT GCTCAAAATC 
ATTGCCTTTA AACCCAAGCA TTACTTTGCC GATGCCTGGA ATACATTCGA TGCTCTTATT 
GTAGTGGGCA GCGTAGTCGA CATTGCCGTC ACAGAAGTCA ATAATGGAGG CCATCTTGGT 
GAGAGCTCAG AGGACAGCTC CCGCATATCT ATCACGTTCT TTCGCCTCTT CCGGGTCATG 
AGGCTGGTGA AGCTGCTCAG TAAGGGTGAG GGGATCCGCA CACTGCTCTG GACATTCATC 
AAGTCTTTCC AGGCCTTGCC CCATGTGGCA CTTCTTATAG CAATGATATT CTTCATATAT 
GCAGTCATTG GCATGCAGAT GTTTGGCAAA GTGGCTCTTC AGGACGGCAC ACAGATAAAT 
CGAAACAATA ATTTCCAGAC CTTTCCGCAG GCTGTGCTGC TTCTGTTCAG GTGTGCCACT 
GGTGAGGCAT GGCAAGAGAT AATGCTAGCC AGCCTTCCAG GAAATCGGTG TGACCCTGAG 
TCTGACTTTG GCCCAGGCGA GGAATTTACC TGTGGTAGCA ATTTTGCCAT TGTCTACTTT 
ATCAGCTTCT TCATGCTCTG TGCCTTCCTG ATTATAAATC TCTTTGTGGC TGTAATCATG 
GATAACTTTG ATTACCTAAC CAGAGATTGG TCTATTCTGG GACCCCACCA CCTCGATGAA 
TTCAAGAGGA TCTGGTCTGA ATATGACCCC GGAGCCAAGG GCCGCATCAA GCACTTGGAT 
GTGGTTGCCC TGCTGAGACG CATCCAGCCT CCACTGGGAT TTGGAAAGCT GTGCCCACAC 
CGAGTGGCCT GCAAGAGACT GGTGGCCATG AATGTTCCCC TCAACTCAGA CGGAACAGTG 
ACATTCAACG CGACACTCTT TGCCCTGGTG CGGACATCCC TGAGGATCAA GACAGAAGGG 
AACCTGGATC AAGCCAACCA GGAGCTTCGG ATGGTCATCA AAAAGATCTG GAAGCGGATA 
AAGCAGAAAT TGTTAGATGA GGTCATCCCC CCTCCAGATG AGGAGGAGGT CACTGTGGGA 
AAATTCTATG CCACATTTCT CATCCAAGAT TATTTCCGAA AATTCCGGAG AAGGAAAGAA 
AAGGGGCTAC TAGGAGCAGA TGCCCCAACA AGCACATCCT CTGTCCTCCA GGCTGGTCTA 
AGGAGCCTAC AGGACTTGGG TCCTGAGATC CGACAGGCCC TCACCTATGC CCTTGAGGAA 
GAAGAAGAAG AAGAAGAGGC CCGCCAGGTC AAGGAAGCTG AGGAGGAGGA AGCTGAGACC 
ACCCCAGAAA CATACAAAGA CTCCATAGAC TCCCAGCCCC AAGCTCAATG GAACTCGAGG 
ATTTCGGTGT CTCTACCCAA GTTAAGAGAA ACTCCAGATT CTCTTTCAAC TGGGCCTAGT 
GATGATGATG GAGTGGCTCC CAACTCCAGG CAACCCAGTG GGCTACAGGC TGGATCCCAA 
CCCCACAGGA GAGGCTCTGG GGTTTTCATG TTCACTATCC CAGAAGAAGG AAGTACTCAG 
CTCAAGGGAG TTCAAGGGCA AGACAATCAG AATGAGGAAC AGGAAGTCCC TGACTGGACC 
CCCAACCTGG ATGAGCAGGC AGGGATGCCC TCCAACCCAG TCCTTTTACC ACCTCACTGG 
TCCCAGCAAC ATGTAAATGG GCACCATGTA CCACGCCGAC GTTTGCTACC CCCCACGCCT 
GCAGGTCGGA AGCCCTCCTT CACCATCCAG TGTCTGCAAC GCCAGGGCAG TTGTGAGGAT 
TTGCCCATCC CAGGCACCTA CCATCGTGGA CGGACCTCAG GACCAGGCAG GGCTCAGGGT 
TCCTGGGCAG CCCCTCCTCA GAAGGGTCGA CTGCTCTATG CCCCCCTGTT GCTGGTGGAG 
GAATCTACAG TGGGTGAAGG ATACCTTGGC AAGCTCGGCG GCCCACTGCG TACCTTCACT 
TGTCTGCAAG TGCCTGGAGC TCCTTCAGAT CCCAGTCACC GCAAGAGGGG CAGTGCTGAC 
AGTTTGGTGG AGGCTGTGCT TATCTCCGAA GGCCTAGGCC TCTTTGCCCA AGACCCACGA 
TTTGTGGCCC TGGCCAAGCA AGAGATTGCA GATGCATGTC ACCTGACCCT GGATGAGATG 
GACAGTGCTG CCAGTGACCT GCTGGCACAG AGAACCACCT CCCTCTACAG TGACGAGGAG 
TCTATTCTCT CCCGCTTTGA TGAAGAGGAC CTGGGAGACG AGATGGCCTG CGTCCATGCC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1f ( XM_017601894.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457383.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X1, mRNA.

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CloneID	ORa62990
Clone ID Related Accession (Same CDS sequence)	XM_017601894.1
Accession Version	XM_017601894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAT CTGAAGTCGG GAAAGACACC ACCCCAGAAC CCAGTCCAGC CAATGGGACC 
GGTCCTGGCC CTGAGTGGGG GCTCTGTCCT GGGCCTCCAA CCGTGGGGAC TGATACCAGT 
GGGGCATCGG GCCTGGGGAC CCCAAGACGG AGGACTCAGC ACAACAAGCA CAAGACCGTA 
GTGGTGGCCA GTGCGCAGAG ATCACCTCGA GCACTCTTCT GCCTCACCTT AACCAATCCC 
ATTCGTCGGT CCTGCATCAG CATTGTGGAG TGGAAGCCAT TTGATATTCT CATCCTCCTG 
ACCATCTTTG CCAACTGTGT GGCATTGGGG GTATATATCC CCTTCCCTGA GGACGACTCC 
AACACTGCTA ACCACAACTT GGAACAGGTA GAATATGTGT TCCTGGTGAT TTTCACCGTG 
GAGACAGTGC TCAAGATCGT GGCCTATGGG CTGGTGCTCC ATCCCAGCGC CTACATTCGC 
AATGGTTGGA ACCTACTCGA CTTCATCATC GTTGTAGTCG GGCTGTTCAG CGTGTTGCTG 
GAGCAGGGAC CTGGGCGGCC AGGAGATGCC CCGCATACTG GAGGAAAGCC GGGAGGCTTT 
GATGTAAAGG CACTGCGGGC ATTTAGGGTG CTACGACCAC TAAGGCTAGT GTCTGGGGTC 
CCGAGTCTGC ACATAGTGCT CAATTCCATC ATGAAGGCGC TTGTGCCGCT TCTGCACATT 
GCCCTGTTGG TGCTCTTCGT CATTATCATT TACGCCATCA TCGGACTCGA GCTGTTCCTC 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCTGATA TGGAGGCAGA GGAGGACCCG 
TCGCCTTGTG CATCTTCTGG CTCTGGCCGT TCATGCACAC TGAACCAGAC TGAGTGCCGT 
GGGCGCTGGC CAGGACCCAA CGGTGGCATC ACGAACTTTG ACAATTTTTT CTTTGCCATG 
CTAACTGTGT TCCAGTGTAT TACCATGGAA GGCTGGACAG ACGTCCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT ACCTTGGGTG TACTTTGTGA GCCTCGTCAT CTTTGGGTCT 
TTCTTTGTCC TCAACCTTGT GCTTGGAGTC CTAAGTGGGG AGTTCTCCAA GGAAAGAGAA 
AAGGCAAAAG CCCGAGGTGA CTTTCAGAAG CTTCGGGAGA AGCAGCAGAT GGAAGAGGAC 
CTTCGGGGCT ACCTGGACTG GATCACACAG GCTGAGGAGT TGGACCTTCA TGACCCCTCA 
GTAGACGGCA ACTTGGCTTC TCTTGCTGAA GAGGGACGGG CAGGCCATCG ACCACAACTG 
TCAGAGCTGA CCAATAGGAG GCGCGGACGA CTGCGTTGGT TCAGCCACTC CACTCGCTCC 
ACACACTCCA CCAGCAGTCA TGCCAGCCTT CCAGCCAGTG ACACTGGTTC CATAACAGAC 
ACCCCCGGAG ATGAGGATGA AGAGGAGGGG ACCATGGCCA GCTGTACACT CTGCCTAAAC 
AAGATTATGA AAACCAAGGT CTGCCGCCAC TTCCGCCGAG CCAACCGTGG TCTCCGTGCA 
CGCTGCCGCC GGGCAGTCAA GTCCAATGCC TGCTACTGGG CTGTATTGCT GCTCGTCTTC 
CTCAACACGT TGACCATCGC TTCTGAGCAC CATGGGCAGC CAGTGTGGCT CACCCAGACC 
CAAGAATATG CCAACAAGGT TCTGCTCTGC CTCTTCACCG TGGAGATGCT TCTCAAACTG 
TACGGCCTGG GCCCCTCTGT CTACGTTGCC TCTTTTTTCA ACCGCTTTGA CTGTTTCGTG 
GTCTGTGGGG GCATCCTAGA AACGACGTTG GTGGAGGTGG GGGCCATGCA GCCCCTCGGC 
ATCTCAGTGC TCCGATGTGT GCGTCTCCTC AGGATCTTCA AGGTCACCAG GCACTGGGCA 
TCCCTCAGCA ATCTGGTGGC ATCGCTGCTC AACTCCATGA AGTCCATCGC CTCCTTGCTG 
CTTCTCCTCT TTCTCTTTAT CATCATCTTC TCCCTTCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACTAAG AGAAGCACCT TTGATACCTT CCCCCAAGCC 
CTCCTCACCG TCTTTCAGAT CCTGACTGGT GAGGATTGGA ATGTAGTCAT GTATGACGGT 
ATCATGGCCT ACGGTGGTCC CTTCTTCCCA GGGATGCTGG TGTGTGTTTA TTTCATCATC 
CTCTTCATCT GTGGCAACTA CATCCTACTG AACGTGTTTC TTGCCATTGC TGTGGATAAC 
CTAGCCAGCG GGGATGCGGG TGCTGCCAAA GATAAGGGCA GAGAGAAGAG CAGTGAAGGA 
AACCCTCCCC AAGAGAACAA AGTATTGGTG CCTGGTGGAG AAAATGAGGA CACAGAGGGC 
ACAAAAAGTG AAGGAGCAGC ACCAGGCATG GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG 
GAGGAAGAAA ATGGTGCAGG ACATGTGGAA CTCCTGCAGG AAGTTGTACC CAAGGAGAAG 
GTGGTACCCA TCCCTGAAGG CAGTGCCTTC TTCTGCCTCA GCCAAACCAA CCCGCTTCGC 
AAGGCCTGCC ACACTCTCAT ACACCATCAC GTGTTCACCA GTCTCATCCT GGTGTTCATC 
ATCCTCAGTA GTGTGTCCCT GGCTGCTGAG GACCCCATCA GAGCCCACTC CTTCCGCAAC 
CATATTCTGG GGTACTTCGA TTATGCCTTC ACTTCCATTT TCACCGTGGA GATTCTATTA 
AAGATGACGG TGTTTGGGGC TTTCCTGCAC CAAGGTTCTT TCTGCCGTAG CTGGTTCAAT 
CTGTTGGATC TGCTTGTGGT CAGTGTGTCC CTCATCTCCT TTGGCATCCA CTCCAGTGCC 
ATCTCAGTTG TGAAGATTCT CCGAGTCCTC CGAGTCCTGC GGCCTCTCCG AGCCATTAAC 
CGAGCCAAGG GACTCAAGCA TGTGGTGCAG TGTGTGTTTG TGGCCATCCG GACCATTGGA 
AACATCATGA TTGTCACCAC CCTCTTGCAG TTCATGTTCG CCTGCATTGG TGTTCAGCTG 
TTCAAGGGAA AATTCTACAG TTGCACTGAT GAAGCCAAAC ACACCCTGAA AGAATGCAAG 
GGCTCCTTCC TCATCTACCC TGATGGAGAT GTGTCACGAC CTTTGGTCCG GGAACGCCTC 
TGGGTCAACA GTGATTTCAA CTTTGACAAC GTCCTTTCAG CCATGATGGC CCTGTTCACT 
GTCTCTACCT TTGAAGGCTG GCCTGCGCTA CTATACAAAG CCATAGATGC ACACGCTGAA 
GACGAGGGCC CTATCTACAA TTACCATGTG GAGATATCAG TATTCTTCAT TGTCTACATC 
ATCATCATCG CCTTCTTCAT GATGAACATC TTTGTGGGAT TTGTCATCAT CACATTCCGT 
GCCCAGGGAG AGCAGGAATA TCAAAACTGT GAACTGGACA AGAACCAGCG CCAGTGTGTG 
GAATATGCAC TCAAAGCTCA GCCACTCCGC CGATACATCC CCAAGAATCC ACATCAGTAC 
CGCGTGTGGG CCACAGTGAA CTCAGCCGCC TTTGAGTACC TCATGTTTCT GCTCATCCTC 
CTCAACACAG TTGCCCTGGC CATGCAGCAC TATGAGCAGA CGGCTCCCTT TAACTATGCC 
ATGGACATAC TCAACATGGT CTTCACTGGC CTCTTCACCG TTGAGATGGT GCTCAAAATC 
ATTGCCTTTA AACCCAAGCA TTACTTTGCC GATGCCTGGA ATACATTCGA TGCTCTTATT 
GTAGTGGGCA GCGTAGTCGA CATTGCCGTC ACAGAAGTCA ATAGCTCAGA GGACAGCTCC 
CGCATATCTA TCACGTTCTT TCGCCTCTTC CGGGTCATGA GGCTGGTGAA GCTGCTCAGT 
AAGGGTGAGG GGATCCGCAC ACTGCTCTGG ACATTCATCA AGTCTTTCCA GGCCTTGCCC 
TATGTGGCAC TTCTTATAGC AATGATATTC TTCATATATG CAGTCATTGG CATGCAGATG 
TTTGGCAAAG TGGCTCTTCA GGACGGCACA CAGATAAATC GAAACAATAA TTTCCAGACC 
TTTCCGCAGG CTGTGCTGCT TCTGTTCAGG TGTGCCACTG GTGAGGCATG GCAAGAGATA 
ATGCTAGCCA GCCTTCCAGG AAATCGGTGT GACCCTGAGT CTGACTTTGG CCCAGGCGAG 
GAATTTACCT GTGGTAGCAA TTTTGCCATT GTCTACTTTA TCAGCTTCTT CATGCTCTGT 
GCCTTCCTGA TTATAAATCT CTTTGTGGCT GTAATCATGG ATAACTTTGA TTACCTAACC 
AGAGATTGGT CTATTCTGGG ACCCCACCAC CTCGATGAAT TCAAGAGGAT CTGGTCTGAA 
TATGACCCCG GAGCCAAGGG CCGCATCAAG CACTTGGATG TGGTTGCCCT GCTGAGACGC 
ATCCAGCCTC CACTGGGATT TGGAAAGCTG TGCCCACACC GAGTGGCCTG CAAGAGACTG 
GTGGCCATGA ATGTTCCCCT CAACTCAGAC GGAACAGTGA CATTCAACGC GACACTCTTT 
GCCCTGGTGC GGACATCCCT GAGGATCAAG ACAGAAGGGA ACCTGGATCA AGCCAACCAG 
GAGCTTCGGA TGGTCATCAA AAAGATCTGG AAGCGGATAA AGCAGAAATT GTTAGATGAG 
GTCATCCCCC CTCCAGATGA GGAGGAGGTC ACTGTGGGAA AATTCTATGC CACATTTCTC 
ATCCAAGATT ATTTCCGAAA ATTCCGGAGA AGGAAAGAAA AGGGGCTACT AGGAGCAGAT 
GCCCCAACAA GCACATCCTC TGTCCTCCAG GCTGGTCTAA GGAGCCTACA GGACTTGGGT 
CCTGAGATCC GACAGGCCCT CACCTATGAC ACTGAGGAAG AAGAAGAAGA AGAAGAGGCC 
GCAGGTCAGG AAGCTGAGGA GGAGGAAGCT GAGAACAACC CAGAAACATA CAAAGACTCC 
ATAGACTCCC AGCCCCAAGC TCAATGGAAC TCGAGGATTT CGGTGTCTCT ACCAGTTAAG 
GAGAAACTCC CAGATTCTCT TTCAACTGGG CCTAGTGATG ATGATGGAGT GGCTCCCAAC 
TCCAGGCAAC CCAGTGGGCT ACAGGCTGGA TCCCAACCCC ACAGGAGAAG CTCTGGGGTT 
TTCATGTTCA CTATCCCAGA AGAAGGAAGT ACTCAGCTCA AGGGAGTTCA AGGGCAAGAC 
AATCAGAATG AGGAACAGGA AGTCCCTGAC TGGACCCCCA ACCTGGATGA GCAGGCAGGG 
ATGCCCTCCA ACCCAGTCCT TTTACCACCT CACTGGTCCC AGCAACATGT AAATGGGCAC 
CATGTACCAC GCCGACGTTT GCTACCCCCC ACGCCTGCAG GTCGGAAGCC CTCCTTCACC 
ATCCAGTGTC TGCAACGCCA GGGCAGTTGT GAGGATTTGC CCATCCCAGG CACCTACCAT 
CGTGGACGGA CCTCAGGACC AGGCAGGGCT CAGGGTTCCT GGGCAGCCCC TCCTCAGAAG 
GGTCGACTGC TCTATGCCCC CCTGTTGCTG GTGGAGGAAT CTACAGTGGG TGAAGGATAC 
CTTGGCAAGC TCGGCGGCCC ACTGCGTACC TTCACTTGTC TGCAAGTGCC TGGAGCTCCT 
TCAGATCCCA GTCACCGCAA GAGGGGCAGT GCTGACAGTT TGGTGGAGGC TGTGCTTATC 
TCCGAAGGCC TAGGCCTCTT TGCCCAAGAC CCACGATTTG TGGCCCTGGC CAAGCAAGAG 
ATTGCAGATG CATGTCACCT GACCCTGGAT GAGATGGACA GTGCTGCCAG TGACCTGCTG 
GCACAGAGAA CCACCTCCCT TTACAGTGAC GAGGAGTCTA TTCTCTCCCG CTTTGATGAA 
GAGGACCTGG GAGATGAGAT GGCCTGTGTC CATGCCCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457383.1
CDS	66..5987
Translation	MSESEVGKDTTPEPSPANGTGPGPEWGLCPGPPTVGTDTSGASGLGTPRRRTQHNKHKTVVVASAQRSPRALFCLTLTNPIRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRSCTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSITDTPGDEDEEEGTMASCTLCLNKIMKTKVCRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGAAKDKGREKSSEGNPPQENKVLVPGGENEDTEGTKSEGAAPGMEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHVFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHQGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAHAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNSSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLRIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGADAPTSTSSVLQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAAGQEAEEEEAENNPETYKDSIDSQPQAQWNSRISVSLPVKEKLPDSLSTGPSDDDGVAPNSRQPSGLQAGSQPHRRSSGVFMFTIPEEGSTQLKGVQGQDNQNEEQEVPDWTPNLDEQAGMPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPGRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAPSDPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_017601894.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601894.1

ORF Insert Sequence:

ATGTCGGAAT CTGAAGTCGG GAAAGACACC ACCCCAGAAC CCAGTCCAGC CAATGGGACC 
GGTCCTGGCC CTGAGTGGGG GCTCTGTCCT GGGCCTCCAA CCGTGGGGAC TGATACCAGT 
GGGGCATCGG GCCTGGGGAC CCCAAGACGG AGGACTCAGC ACAACAAGCA CAAGACCGTA 
GTGGTGGCCA GTGCGCAGAG ATCACCTCGA GCACTCTTCT GCCTCACCTT AACCAATCCC 
ATTCGTCGGT CCTGCATCAG CATTGTGGAG TGGAAGCCAT TTGATATTCT CATCCTCCTG 
ACCATCTTTG CCAACTGTGT GGCATTGGGG GTATATATCC CCTTCCCTGA GGACGACTCC 
AACACTGCTA ACCACAACTT GGAACAGGTA GAATATGTGT TCCTGGTGAT TTTCACCGTG 
GAGACAGTGC TCAAGATCGT GGCCTATGGG CTGGTGCTCC ATCCCAGCGC CTACATTCGC 
AATGGTTGGA ACCTACTCGA CTTCATCATC GTTGTAGTCG GGCTGTTCAG CGTGTTGCTG 
GAGCAGGGAC CTGGGCGGCC AGGAGATGCC CCGCATACTG GAGGAAAGCC GGGAGGCTTT 
GATGTAAAGG CACTGCGGGC ATTTAGGGTG CTACGACCAC TAAGGCTAGT GTCTGGGGTC 
CCGAGTCTGC ACATAGTGCT CAATTCCATC ATGAAGGCGC TTGTGCCGCT TCTGCACATT 
GCCCTGTTGG TGCTCTTCGT CATTATCATT TACGCCATCA TCGGACTCGA GCTGTTCCTC 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCTGATA TGGAGGCAGA GGAGGACCCG 
TCGCCTTGTG CATCTTCTGG CTCTGGCCGT TCATGCACAC TGAACCAGAC TGAGTGCCGT 
GGGCGCTGGC CAGGACCCAA CGGTGGCATC ACGAACTTTG ACAATTTTTT CTTTGCCATG 
CTAACTGTGT TCCAGTGTAT TACCATGGAA GGCTGGACAG ACGTCCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT ACCTTGGGTG TACTTTGTGA GCCTCGTCAT CTTTGGGTCT 
TTCTTTGTCC TCAACCTTGT GCTTGGAGTC CTAAGTGGGG AGTTCTCCAA GGAAAGAGAA 
AAGGCAAAAG CCCGAGGTGA CTTTCAGAAG CTTCGGGAGA AGCAGCAGAT GGAAGAGGAC 
CTTCGGGGCT ACCTGGACTG GATCACACAG GCTGAGGAGT TGGACCTTCA TGACCCCTCA 
GTAGACGGCA ACTTGGCTTC TCTTGCTGAA GAGGGACGGG CAGGCCATCG ACCACAACTG 
TCAGAGCTGA CCAATAGGAG GCGCGGACGA CTGCGTTGGT TCAGCCACTC CACTCGCTCC 
ACACACTCCA CCAGCAGTCA TGCCAGCCTT CCAGCCAGTG ACACTGGTTC CATAACAGAC 
ACCCCCGGAG ATGAGGATGA AGAGGAGGGG ACCATGGCCA GCTGTACACT CTGCCTAAAC 
AAGATTATGA AAACCAAGGT CTGCCGCCAC TTCCGCCGAG CCAACCGTGG TCTCCGTGCA 
CGCTGCCGCC GGGCAGTCAA GTCCAATGCC TGCTACTGGG CTGTATTGCT GCTCGTCTTC 
CTCAACACGT TGACCATCGC TTCTGAGCAC CATGGGCAGC CAGTGTGGCT CACCCAGACC 
CAAGAATATG CCAACAAGGT TCTGCTCTGC CTCTTCACCG TGGAGATGCT TCTCAAACTG 
TACGGCCTGG GCCCCTCTGT CTACGTTGCC TCTTTTTTCA ACCGCTTTGA CTGTTTCGTG 
GTCTGTGGGG GCATCCTAGA AACGACGTTG GTGGAGGTGG GGGCCATGCA GCCCCTCGGC 
ATCTCAGTGC TCCGATGTGT GCGTCTCCTC AGGATCTTCA AGGTCACCAG GCACTGGGCA 
TCCCTCAGCA ATCTGGTGGC ATCGCTGCTC AACTCCATGA AGTCCATCGC CTCCTTGCTG 
CTTCTCCTCT TTCTCTTTAT CATCATCTTC TCCCTTCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACTAAG AGAAGCACCT TTGATACCTT CCCCCAAGCC 
CTCCTCACCG TCTTTCAGAT CCTGACTGGT GAGGATTGGA ATGTAGTCAT GTATGACGGT 
ATCATGGCCT ACGGTGGTCC CTTCTTCCCA GGGATGCTGG TGTGTGTTTA TTTCATCATC 
CTCTTCATCT GTGGCAACTA CATCCTACTG AACGTGTTTC TTGCCATTGC TGTGGATAAC 
CTAGCCAGCG GGGATGCGGG TGCTGCCAAA GATAAGGGCA GAGAGAAGAG CAGTGAAGGA 
AACCCTCCCC AAGAGAACAA AGTATTGGTG CCTGGTGGAG AAAATGAGGA CACAGAGGGC 
ACAAAAAGTG AAGGAGCAGC ACCAGGCATG GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG 
GAGGAAGAAA ATGGTGCAGG ACATGTGGAA CTCCTGCAGG AAGTTGTACC CAAGGAGAAG 
GTGGTACCCA TCCCTGAAGG CAGTGCCTTC TTCTGCCTCA GCCAAACCAA CCCGCTTCGC 
AAGGCCTGCC ACACTCTCAT ACACCATCAC GTGTTCACCA GTCTCATCCT GGTGTTCATC 
ATCCTCAGTA GTGTGTCCCT GGCTGCTGAG GACCCCATCA GAGCCCACTC CTTCCGCAAC 
CATATTCTGG GGTACTTCGA TTATGCCTTC ACTTCCATTT TCACCGTGGA GATTCTATTA 
AAGATGACGG TGTTTGGGGC TTTCCTGCAC CAAGGTTCTT TCTGCCGTAG CTGGTTCAAT 
CTGTTGGATC TGCTTGTGGT CAGTGTGTCC CTCATCTCCT TTGGCATCCA CTCCAGTGCC 
ATCTCAGTTG TGAAGATTCT CCGAGTCCTC CGAGTCCTGC GGCCTCTCCG AGCCATTAAC 
CGAGCCAAGG GACTCAAGCA TGTGGTGCAG TGTGTGTTTG TGGCCATCCG GACCATTGGA 
AACATCATGA TTGTCACCAC CCTCTTGCAG TTCATGTTCG CCTGCATTGG TGTTCAGCTG 
TTCAAGGGAA AATTCTACAG TTGCACTGAT GAAGCCAAAC ACACCCTGAA AGAATGCAAG 
GGCTCCTTCC TCATCTACCC TGATGGAGAT GTGTCACGAC CTTTGGTCCG GGAACGCCTC 
TGGGTCAACA GTGATTTCAA CTTTGACAAC GTCCTTTCAG CCATGATGGC CCTGTTCACT 
GTCTCTACCT TTGAAGGCTG GCCTGCGCTA CTATACAAAG CCATAGATGC ACACGCTGAA 
GACGAGGGCC CTATCTACAA TTACCATGTG GAGATATCAG TATTCTTCAT TGTCTACATC 
ATCATCATCG CCTTCTTCAT GATGAACATC TTTGTGGGAT TTGTCATCAT CACATTCCGT 
GCCCAGGGAG AGCAGGAATA TCAAAACTGT GAACTGGACA AGAACCAGCG CCAGTGTGTG 
GAATATGCAC TCAAAGCTCA GCCACTCCGC CGATACATCC CCAAGAATCC ACATCAGTAC 
CGCGTGTGGG CCACAGTGAA CTCAGCCGCC TTTGAGTACC TCATGTTTCT GCTCATCCTC 
CTCAACACAG TTGCCCTGGC CATGCAGCAC TATGAGCAGA CGGCTCCCTT TAACTATGCC 
ATGGACATAC TCAACATGGT CTTCACTGGC CTCTTCACCG TTGAGATGGT GCTCAAAATC 
ATTGCCTTTA AACCCAAGCA TTACTTTGCC GATGCCTGGA ATACATTCGA TGCTCTTATT 
GTAGTGGGCA GCGTAGTCGA CATTGCCGTC ACAGAAGTCA ATAGCTCAGA GGACAGCTCC 
CGCATATCTA TCACGTTCTT TCGCCTCTTC CGGGTCATGA GGCTGGTGAA GCTGCTCAGT 
AAGGGTGAGG GGATCCGCAC ACTGCTCTGG ACATTCATCA AGTCTTTCCA GGCCTTGCCC 
TATGTGGCAC TTCTTATAGC AATGATATTC TTCATATATG CAGTCATTGG CATGCAGATG 
TTTGGCAAAG TGGCTCTTCA GGACGGCACA CAGATAAATC GAAACAATAA TTTCCAGACC 
TTTCCGCAGG CTGTGCTGCT TCTGTTCAGG TGTGCCACTG GTGAGGCATG GCAAGAGATA 
ATGCTAGCCA GCCTTCCAGG AAATCGGTGT GACCCTGAGT CTGACTTTGG CCCAGGCGAG 
GAATTTACCT GTGGTAGCAA TTTTGCCATT GTCTACTTTA TCAGCTTCTT CATGCTCTGT 
GCCTTCCTGA TTATAAATCT CTTTGTGGCT GTAATCATGG ATAACTTTGA TTACCTAACC 
AGAGATTGGT CTATTCTGGG ACCCCACCAC CTCGATGAAT TCAAGAGGAT CTGGTCTGAA 
TATGACCCCG GAGCCAAGGG CCGCATCAAG CACTTGGATG TGGTTGCCCT GCTGAGACGC 
ATCCAGCCTC CACTGGGATT TGGAAAGCTG TGCCCACACC GAGTGGCCTG CAAGAGACTG 
GTGGCCATGA ATGTTCCCCT CAACTCAGAC GGAACAGTGA CATTCAACGC GACACTCTTT 
GCCCTGGTGC GGACATCCCT GAGGATCAAG ACAGAAGGGA ACCTGGATCA AGCCAACCAG 
GAGCTTCGGA TGGTCATCAA AAAGATCTGG AAGCGGATAA AGCAGAAATT GTTAGATGAG 
GTCATCCCCC CTCCAGATGA GGAGGAGGTC ACTGTGGGAA AATTCTATGC CACATTTCTC 
ATCCAAGATT ATTTCCGAAA ATTCCGGAGA AGGAAAGAAA AGGGGCTACT AGGAGCAGAT 
GCCCCAACAA GCACATCCTC TGTCCTCCAG GCTGGTCTAA GGAGCCTACA GGACTTGGGT 
CCTGAGATCC GACAGGCCCT CACCTATGAC ACTGAGGAAG AAGAAGAAGA AGAAGAGGCC 
GCAGGTCAGG AAGCTGAGGA GGAGGAAGCT GAGAACAACC CAGAAACATA CAAAGACTCC 
ATAGACTCCC AGCCCCAAGC TCAATGGAAC TCGAGGATTT CGGTGTCTCT ACCAGTTAAG 
GAGAAACTCC CAGATTCTCT TTCAACTGGG CCTAGTGATG ATGATGGAGT GGCTCCCAAC 
TCCAGGCAAC CCAGTGGGCT ACAGGCTGGA TCCCAACCCC ACAGGAGAAG CTCTGGGGTT 
TTCATGTTCA CTATCCCAGA AGAAGGAAGT ACTCAGCTCA AGGGAGTTCA AGGGCAAGAC 
AATCAGAATG AGGAACAGGA AGTCCCTGAC TGGACCCCCA ACCTGGATGA GCAGGCAGGG 
ATGCCCTCCA ACCCAGTCCT TTTACCACCT CACTGGTCCC AGCAACATGT AAATGGGCAC 
CATGTACCAC GCCGACGTTT GCTACCCCCC ACGCCTGCAG GTCGGAAGCC CTCCTTCACC 
ATCCAGTGTC TGCAACGCCA GGGCAGTTGT GAGGATTTGC CCATCCCAGG CACCTACCAT 
CGTGGACGGA CCTCAGGACC AGGCAGGGCT CAGGGTTCCT GGGCAGCCCC TCCTCAGAAG 
GGTCGACTGC TCTATGCCCC CCTGTTGCTG GTGGAGGAAT CTACAGTGGG TGAAGGATAC 
CTTGGCAAGC TCGGCGGCCC ACTGCGTACC TTCACTTGTC TGCAAGTGCC TGGAGCTCCT 
TCAGATCCCA GTCACCGCAA GAGGGGCAGT GCTGACAGTT TGGTGGAGGC TGTGCTTATC 
TCCGAAGGCC TAGGCCTCTT TGCCCAAGAC CCACGATTTG TGGCCCTGGC CAAGCAAGAG 
ATTGCAGATG CATGTCACCT GACCCTGGAT GAGATGGACA GTGCTGCCAG TGACCTGCTG 
GCACAGAGAA CCACCTCCCT TTACAGTGAC GAGGAGTCTA TTCTCTCCCG CTTTGATGAA 
GAGGACCTGG GAGATGAGAT GGCCTGTGTC CATGCCCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1f ( XM_017601896.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457385.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X4, mRNA.

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CloneID	ORa62992
Clone ID Related Accession (Same CDS sequence)	XM_017601896.1
Accession Version	XM_017601896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAT CTGAAGTCGG GAAAGACACC ACCCCAGAAC CCAGTCCAGC CAATGGGACC 
GGTCCTGGCC CTGAGTGGGG GCTCTGTCCT GGGCCTCCAA CCGTGGGGAC TGATACCAGT 
GGGGCATCGG GCCTGGGGAC CCCAAGACGG AGGACTCAGC ACAACAAGCA CAAGACCGTA 
GTGGTGGCCA GTGCGCAGAG ATCACCTCGA GCACTCTTCT GCCTCACCTT AACCAATCCC 
ATTCGTCGGT CCTGCATCAG CATTGTGGAG TGGAAGCCAT TTGATATTCT CATCCTCCTG 
ACCATCTTTG CCAACTGTGT GGCATTGGGG GTATATATCC CCTTCCCTGA GGACGACTCC 
AACACTGCTA ACCACAACTT GGAACAGGTA GAATATGTGT TCCTGGTGAT TTTCACCGTG 
GAGACAGTGC TCAAGATCGT GGCCTATGGG CTGGTGCTCC ATCCCAGCGC CTACATTCGC 
AATGGTTGGA ACCTACTCGA CTTCATCATC GTTGTAGTCG GGCTGTTCAG CGTGTTGCTG 
GAGCAGGGAC CTGGGCGGCC AGGAGATGCC CCGCATACTG GAGGAAAGCC GGGAGGCTTT 
GATGTAAAGG CACTGCGGGC ATTTAGGGTG CTACGACCAC TAAGGCTAGT GTCTGGGGTC 
CCGAGTCTGC ACATAGTGCT CAATTCCATC ATGAAGGCGC TTGTGCCGCT TCTGCACATT 
GCCCTGTTGG TGCTCTTCGT CATTATCATT TACGCCATCA TCGGACTCGA GCTGTTCCTC 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCTGATA TGGAGGCAGA GGAGGACCCG 
TCGCCTTGTG CATCTTCTGG CTCTGGCCGT TCATGCACAC TGAACCAGAC TGAGTGCCGT 
GGGCGCTGGC CAGGACCCAA CGGTGGCATC ACGAACTTTG ACAATTTTTT CTTTGCCATG 
CTAACTGTGT TCCAGTGTAT TACCATGGAA GGCTGGACAG ACGTCCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT ACCTTGGGTG TACTTTGTGA GCCTCGTCAT CTTTGGGTCT 
TTCTTTGTCC TCAACCTTGT GCTTGGAGTC CTAAGTGGGG AGTTCTCCAA GGAAAGAGAA 
AAGGCAAAAG CCCGAGGTGA CTTTCAGAAG CTTCGGGAGA AGCAGCAGAT GGAAGAGGAC 
CTTCGGGGCT ACCTGGACTG GATCACACAG GCTGAGGAGT TGGACCTTCA TGACCCCTCA 
GTAGACGGCA ACTTGGCTTC TCTTGCTGAA GAGGGACGGG CAGGCCATCG ACCACAACTG 
TCAGAGCTGA CCAATAGGAG GCGCGGACGA CTGCGTTGGT TCAGCCACTC CACTCGCTCC 
ACACACTCCA CCAGCAGTCA TGCCAGCCTT CCAGCCAGTG ACACTGGTTC CATAACAGAC 
ACCCCCGGAG ATGAGGATGA AGAGGAGGGG ACCATGGCCA GCTGTACACT CTGCCTAAAC 
AAGATTATGA AAACCAAGGT CTGCCGCCAC TTCCGCCGAG CCAACCGTGG TCTCCGTGCA 
CGCTGCCGCC GGGCAGTCAA GTCCAATGCC TGCTACTGGG CTGTATTGCT GCTCGTCTTC 
CTCAACACGT TGACCATCGC TTCTGAGCAC CATGGGCAGC CAGTGTGGCT CACCCAGACC 
CAAGAATATG CCAACAAGGT TCTGCTCTGC CTCTTCACCG TGGAGATGCT TCTCAAACTG 
TACGGCCTGG GCCCCTCTGT CTACGTTGCC TCTTTTTTCA ACCGCTTTGA CTGTTTCGTG 
GTCTGTGGGG GCATCCTAGA AACGACGTTG GTGGAGGTGG GGGCCATGCA GCCCCTCGGC 
ATCTCAGTGC TCCGATGTGT GCGTCTCCTC AGGATCTTCA AGGTCACCAG GCACTGGGCA 
TCCCTCAGCA ATCTGGTGGC ATCGCTGCTC AACTCCATGA AGTCCATCGC CTCCTTGCTG 
CTTCTCCTCT TTCTCTTTAT CATCATCTTC TCCCTTCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACTAAG AGAAGCACCT TTGATACCTT CCCCCAAGCC 
CTCCTCACCG TCTTTCAGAT CCTGACTGGT GAGGATTGGA ATGTAGTCAT GTATGACGGT 
ATCATGGCCT ACGGTGGTCC CTTCTTCCCA GGGATGCTGG TGTGTGTTTA TTTCATCATC 
CTCTTCATCT GTGGCAACTA CATCCTACTG AACGTGTTTC TTGCCATTGC TGTGGATAAC 
CTAGCCAGCG GGGATGCGGG TGCTGCCAAA GATAAGGGCA GAGAGAAGAG CAGTGAAGGA 
AACCCTCCCC AAGAGAACAA AGTATTGGTG CCTGGTGGAG AAAATGAGGA CACAGAGGGC 
ACAAAAAGTG AAGGAGCAGC ACCAGGCATG GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG 
GAGGAAGAAA ATGGTGCAGG ACATGTGGAA CTCCTGCAGG AAGTTGTACC CAAGGAGAAG 
GTGGTACCCA TCCCTGAAGG CAGTGCCTTC TTCTGCCTCA GCCAAACCAA CCCGCTTCGC 
AAGGCCTGCC ACACTCTCAT ACACCATCAC GTGTTCACCA GTCTCATCCT GGTGTTCATC 
ATCCTCAGTA GTGTGTCCCT GGCTGCTGAG GACCCCATCA GAGCCCACTC CTTCCGCAAC 
CATATTCTGG GGTACTTCGA TTATGCCTTC ACTTCCATTT TCACCGTGGA GATTCTATTA 
AAGATGACGG TGTTTGGGGC TTTCCTGCAC CAAGGTTCTT TCTGCCGTAG CTGGTTCAAT 
CTGTTGGATC TGCTTGTGGT CAGTGTGTCC CTCATCTCCT TTGGCATCCA CTCCAGTGCC 
ATCTCAGTTG TGAAGATTCT CCGAGTCCTC CGAGTCCTGC GGCCTCTCCG AGCCATTAAC 
CGAGCCAAGG GACTCAAGCA TGTGGTGCAG TGTGTGTTTG TGGCCATCCG GACCATTGGA 
AACATCATGA TTGTCACCAC CCTCTTGCAG TTCATGTTCG CCTGCATTGG TGTTCAGCTG 
TTCAAGGGAA AATTCTACAG TTGCACTGAT GAAGCCAAAC ACACCCTGAA AGAATGCAAG 
GGCTCCTTCC TCATCTACCC TGATGGAGAT GTGTCACGAC CTTTGGTCCG GGAACGCCTC 
TGGGTCAACA GTGATTTCAA CTTTGACAAC GTCCTTTCAG CCATGATGGC CCTGTTCACT 
GTCTCTACCT TTGAAGGCTG GCCTGCGCTA CTATACAAAG CCATAGATGC ACACGCTGAA 
GACGAGGGCC CTATCTACAA TTACCATGTG GAGATATCAG TATTCTTCAT TGTCTACATC 
ATCATCATCG CCTTCTTCAT GATGAACATC TTTGTGGGAT TTGTCATCAT CACATTCCGT 
GCCCAGGGAG AGCAGGAATA TCAAAACTGT GAACTGGACA AGAACCAGCG CCAGTGTGTG 
GAATATGCAC TCAAAGCTCA GCCACTCCGC CGATACATCC CCAAGAATCC ACATCAGTAC 
CGCGTGTGGG CCACAGTGAA CTCAGCCGCC TTTGAGTACC TCATGTTTCT GCTCATCCTC 
CTCAACACAG TTGCCCTGGC CATGCAGCAC TATGAGCAGA CGGCTCCCTT TAACTATGCC 
ATGGACATAC TCAACATGGT CTTCACTGGC CTCTTCACCG TTGAGATGGT GCTCAAAATC 
ATTGCCTTTA AACCCAAGCA TTACTTTGCC GATGCCTGGA ATACATTCGA TGCTCTTATT 
GTAGTGGGCA GCGTAGTCGA CATTGCCGTC ACAGAAGTCA ATAATGGAGG CCATCTTGGT 
GAGAGCTCAG AGGACAGCTC CCGCATATCT ATCACGTTCT TTCGCCTCTT CCGGGTCATG 
AGGCTGGTGA AGCTGCTCAG TAAGGGTGAG GGGATCCGCA CACTGCTCTG GACATTCATC 
AAGTCTTTCC AGGCCTTGCC CTATGTGGCA CTTCTTATAG CAATGATATT CTTCATATAT 
GCAGTCATTG GCATGCAGAT GTTTGGCAAA GTGGCTCTTC AGGACGGCAC ACAGATAAAT 
CGAAACAATA ATTTCCAGAC CTTTCCGCAG GCTGTGCTGC TTCTGTTCAG GTGTGCCACT 
GGTGAGGCAT GGCAAGAGAT AATGCTAGCC AGCCTTCCAG GAAATCGGTG TGACCCTGAG 
TCTGACTTTG GCCCAGGCGA GGAATTTACC TGTGGTAGCA ATTTTGCCAT TGTCTACTTT 
ATCAGCTTCT TCATGCTCTG TGCCTTCCTG ATTATAAATC TCTTTGTGGC TGTAATCATG 
GATAACTTTG ATTACCTAAC CAGAGATTGG TCTATTCTGG GACCCCACCA CCTCGATGAA 
TTCAAGAGGA TCTGGTCTGA ATATGACCCC GGAGCCAAGG GCCGCATCAA GCACTTGGAT 
GTGGTTGCCC TGCTGAGACG CATCCAGCCT CCACTGGGAT TTGGAAAGCT GTGCCCACAC 
CGAGTGGCCT GCAAGAGACT GGTGGCCATG AATGTTCCCA GAGTGAGTTT TGTGTCTGTG 
CTGTTCATAG AGACTGGTGG CCATGAATGT TCCCAGAGTG AGTTTTGTGT CTGTGCTGTT 
CATAGAGACT GGTGGCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457385.1
CDS	66..4706
Translation	MSESEVGKDTTPEPSPANGTGPGPEWGLCPGPPTVGTDTSGASGLGTPRRRTQHNKHKTVVVASAQRSPRALFCLTLTNPIRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRSCTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSITDTPGDEDEEEGTMASCTLCLNKIMKTKVCRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGAAKDKGREKSSEGNPPQENKVLVPGGENEDTEGTKSEGAAPGMEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHVFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHQGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAHAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPRVSFVSVLFIETGGHECSQSEFCVCAVHRDWWP

Target ORF information:

RefSeq Version	XM_017601896.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 F (Cacna1f), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601896.1

ORF Insert Sequence:

ATGTCGGAAT CTGAAGTCGG GAAAGACACC ACCCCAGAAC CCAGTCCAGC CAATGGGACC 
GGTCCTGGCC CTGAGTGGGG GCTCTGTCCT GGGCCTCCAA CCGTGGGGAC TGATACCAGT 
GGGGCATCGG GCCTGGGGAC CCCAAGACGG AGGACTCAGC ACAACAAGCA CAAGACCGTA 
GTGGTGGCCA GTGCGCAGAG ATCACCTCGA GCACTCTTCT GCCTCACCTT AACCAATCCC 
ATTCGTCGGT CCTGCATCAG CATTGTGGAG TGGAAGCCAT TTGATATTCT CATCCTCCTG 
ACCATCTTTG CCAACTGTGT GGCATTGGGG GTATATATCC CCTTCCCTGA GGACGACTCC 
AACACTGCTA ACCACAACTT GGAACAGGTA GAATATGTGT TCCTGGTGAT TTTCACCGTG 
GAGACAGTGC TCAAGATCGT GGCCTATGGG CTGGTGCTCC ATCCCAGCGC CTACATTCGC 
AATGGTTGGA ACCTACTCGA CTTCATCATC GTTGTAGTCG GGCTGTTCAG CGTGTTGCTG 
GAGCAGGGAC CTGGGCGGCC AGGAGATGCC CCGCATACTG GAGGAAAGCC GGGAGGCTTT 
GATGTAAAGG CACTGCGGGC ATTTAGGGTG CTACGACCAC TAAGGCTAGT GTCTGGGGTC 
CCGAGTCTGC ACATAGTGCT CAATTCCATC ATGAAGGCGC TTGTGCCGCT TCTGCACATT 
GCCCTGTTGG TGCTCTTCGT CATTATCATT TACGCCATCA TCGGACTCGA GCTGTTCCTC 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCTGATA TGGAGGCAGA GGAGGACCCG 
TCGCCTTGTG CATCTTCTGG CTCTGGCCGT TCATGCACAC TGAACCAGAC TGAGTGCCGT 
GGGCGCTGGC CAGGACCCAA CGGTGGCATC ACGAACTTTG ACAATTTTTT CTTTGCCATG 
CTAACTGTGT TCCAGTGTAT TACCATGGAA GGCTGGACAG ACGTCCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT ACCTTGGGTG TACTTTGTGA GCCTCGTCAT CTTTGGGTCT 
TTCTTTGTCC TCAACCTTGT GCTTGGAGTC CTAAGTGGGG AGTTCTCCAA GGAAAGAGAA 
AAGGCAAAAG CCCGAGGTGA CTTTCAGAAG CTTCGGGAGA AGCAGCAGAT GGAAGAGGAC 
CTTCGGGGCT ACCTGGACTG GATCACACAG GCTGAGGAGT TGGACCTTCA TGACCCCTCA 
GTAGACGGCA ACTTGGCTTC TCTTGCTGAA GAGGGACGGG CAGGCCATCG ACCACAACTG 
TCAGAGCTGA CCAATAGGAG GCGCGGACGA CTGCGTTGGT TCAGCCACTC CACTCGCTCC 
ACACACTCCA CCAGCAGTCA TGCCAGCCTT CCAGCCAGTG ACACTGGTTC CATAACAGAC 
ACCCCCGGAG ATGAGGATGA AGAGGAGGGG ACCATGGCCA GCTGTACACT CTGCCTAAAC 
AAGATTATGA AAACCAAGGT CTGCCGCCAC TTCCGCCGAG CCAACCGTGG TCTCCGTGCA 
CGCTGCCGCC GGGCAGTCAA GTCCAATGCC TGCTACTGGG CTGTATTGCT GCTCGTCTTC 
CTCAACACGT TGACCATCGC TTCTGAGCAC CATGGGCAGC CAGTGTGGCT CACCCAGACC 
CAAGAATATG CCAACAAGGT TCTGCTCTGC CTCTTCACCG TGGAGATGCT TCTCAAACTG 
TACGGCCTGG GCCCCTCTGT CTACGTTGCC TCTTTTTTCA ACCGCTTTGA CTGTTTCGTG 
GTCTGTGGGG GCATCCTAGA AACGACGTTG GTGGAGGTGG GGGCCATGCA GCCCCTCGGC 
ATCTCAGTGC TCCGATGTGT GCGTCTCCTC AGGATCTTCA AGGTCACCAG GCACTGGGCA 
TCCCTCAGCA ATCTGGTGGC ATCGCTGCTC AACTCCATGA AGTCCATCGC CTCCTTGCTG 
CTTCTCCTCT TTCTCTTTAT CATCATCTTC TCCCTTCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACTAAG AGAAGCACCT TTGATACCTT CCCCCAAGCC 
CTCCTCACCG TCTTTCAGAT CCTGACTGGT GAGGATTGGA ATGTAGTCAT GTATGACGGT 
ATCATGGCCT ACGGTGGTCC CTTCTTCCCA GGGATGCTGG TGTGTGTTTA TTTCATCATC 
CTCTTCATCT GTGGCAACTA CATCCTACTG AACGTGTTTC TTGCCATTGC TGTGGATAAC 
CTAGCCAGCG GGGATGCGGG TGCTGCCAAA GATAAGGGCA GAGAGAAGAG CAGTGAAGGA 
AACCCTCCCC AAGAGAACAA AGTATTGGTG CCTGGTGGAG AAAATGAGGA CACAGAGGGC 
ACAAAAAGTG AAGGAGCAGC ACCAGGCATG GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG 
GAGGAAGAAA ATGGTGCAGG ACATGTGGAA CTCCTGCAGG AAGTTGTACC CAAGGAGAAG 
GTGGTACCCA TCCCTGAAGG CAGTGCCTTC TTCTGCCTCA GCCAAACCAA CCCGCTTCGC 
AAGGCCTGCC ACACTCTCAT ACACCATCAC GTGTTCACCA GTCTCATCCT GGTGTTCATC 
ATCCTCAGTA GTGTGTCCCT GGCTGCTGAG GACCCCATCA GAGCCCACTC CTTCCGCAAC 
CATATTCTGG GGTACTTCGA TTATGCCTTC ACTTCCATTT TCACCGTGGA GATTCTATTA 
AAGATGACGG TGTTTGGGGC TTTCCTGCAC CAAGGTTCTT TCTGCCGTAG CTGGTTCAAT 
CTGTTGGATC TGCTTGTGGT CAGTGTGTCC CTCATCTCCT TTGGCATCCA CTCCAGTGCC 
ATCTCAGTTG TGAAGATTCT CCGAGTCCTC CGAGTCCTGC GGCCTCTCCG AGCCATTAAC 
CGAGCCAAGG GACTCAAGCA TGTGGTGCAG TGTGTGTTTG TGGCCATCCG GACCATTGGA 
AACATCATGA TTGTCACCAC CCTCTTGCAG TTCATGTTCG CCTGCATTGG TGTTCAGCTG 
TTCAAGGGAA AATTCTACAG TTGCACTGAT GAAGCCAAAC ACACCCTGAA AGAATGCAAG 
GGCTCCTTCC TCATCTACCC TGATGGAGAT GTGTCACGAC CTTTGGTCCG GGAACGCCTC 
TGGGTCAACA GTGATTTCAA CTTTGACAAC GTCCTTTCAG CCATGATGGC CCTGTTCACT 
GTCTCTACCT TTGAAGGCTG GCCTGCGCTA CTATACAAAG CCATAGATGC ACACGCTGAA 
GACGAGGGCC CTATCTACAA TTACCATGTG GAGATATCAG TATTCTTCAT TGTCTACATC 
ATCATCATCG CCTTCTTCAT GATGAACATC TTTGTGGGAT TTGTCATCAT CACATTCCGT 
GCCCAGGGAG AGCAGGAATA TCAAAACTGT GAACTGGACA AGAACCAGCG CCAGTGTGTG 
GAATATGCAC TCAAAGCTCA GCCACTCCGC CGATACATCC CCAAGAATCC ACATCAGTAC 
CGCGTGTGGG CCACAGTGAA CTCAGCCGCC TTTGAGTACC TCATGTTTCT GCTCATCCTC 
CTCAACACAG TTGCCCTGGC CATGCAGCAC TATGAGCAGA CGGCTCCCTT TAACTATGCC 
ATGGACATAC TCAACATGGT CTTCACTGGC CTCTTCACCG TTGAGATGGT GCTCAAAATC 
ATTGCCTTTA AACCCAAGCA TTACTTTGCC GATGCCTGGA ATACATTCGA TGCTCTTATT 
GTAGTGGGCA GCGTAGTCGA CATTGCCGTC ACAGAAGTCA ATAATGGAGG CCATCTTGGT 
GAGAGCTCAG AGGACAGCTC CCGCATATCT ATCACGTTCT TTCGCCTCTT CCGGGTCATG 
AGGCTGGTGA AGCTGCTCAG TAAGGGTGAG GGGATCCGCA CACTGCTCTG GACATTCATC 
AAGTCTTTCC AGGCCTTGCC CTATGTGGCA CTTCTTATAG CAATGATATT CTTCATATAT 
GCAGTCATTG GCATGCAGAT GTTTGGCAAA GTGGCTCTTC AGGACGGCAC ACAGATAAAT 
CGAAACAATA ATTTCCAGAC CTTTCCGCAG GCTGTGCTGC TTCTGTTCAG GTGTGCCACT 
GGTGAGGCAT GGCAAGAGAT AATGCTAGCC AGCCTTCCAG GAAATCGGTG TGACCCTGAG 
TCTGACTTTG GCCCAGGCGA GGAATTTACC TGTGGTAGCA ATTTTGCCAT TGTCTACTTT 
ATCAGCTTCT TCATGCTCTG TGCCTTCCTG ATTATAAATC TCTTTGTGGC TGTAATCATG 
GATAACTTTG ATTACCTAAC CAGAGATTGG TCTATTCTGG GACCCCACCA CCTCGATGAA 
TTCAAGAGGA TCTGGTCTGA ATATGACCCC GGAGCCAAGG GCCGCATCAA GCACTTGGAT 
GTGGTTGCCC TGCTGAGACG CATCCAGCCT CCACTGGGAT TTGGAAAGCT GTGCCCACAC 
CGAGTGGCCT GCAAGAGACT GGTGGCCATG AATGTTCCCA GAGTGAGTTT TGTGTCTGTG 
CTGTTCATAG AGACTGGTGG CCATGAATGT TCCCAGAGTG AGTTTTGTGT CTGTGCTGTT 
CATAGAGACT GGTGGCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of C-terminal Splice Variants of Cav1.4 Ca2+ Channels in Human Retina.
The Journal of biological chemistry291(30)15663-73(2016 07)
Haeseleer F,Williams B,Lee A

Cacna1f gene decreased contractility of skeletal muscle in rat model with congenital stationary night blindness.
Gene562(2)210-9(2015 May)
An J,Zhang L,Jiao B,Lu F,Xia F,Yu Z,Zhang Z

Visual signal pathway reorganization in the Cacna1f mutant rat model.
Investigative ophthalmology & visual science54(3)1988-97(2013 Mar)
Tao Y,Chen T,Liu B,Xue JH,Zhang L,Xia F,Pang JJ,Zhang ZM

Behavioral phenotypic properties of a natural occurring rat model of congenital stationary night blindness with Cacna1f mutation.
Journal of neurogenetics26(3-4)363-73(2012 Sep)
An J,Wang L,Guo Q,Li L,Xia F,Zhang Z

Retinal horizontal cells reduced in a rat model of congenital stationary night blindness.
Neuroscience letters521(1)26-30(2012 Jul)
Zheng L,Yan Y,An J,Zhang L,Liu W,Xia F,Zhang Z

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Cacna1f cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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