Cacna1f cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1f
Entrez Gene ID	54652
Full Name	calcium channel, voltage-dependent, alpha 1F subunit
Synonyms	A930034B14,Cav1.4,Sfc17,nerg1,nob2
General protein information	Preferred Names calcium channel, voltage-dependent, alpha 1F subunit Names voltage-dependent L-type calcium channel subunit alpha-1F L-type dihydropyridine-sensitive calcium channel alpha-1f subunit voltage-gated calcium channel subunit alpha Cav1.4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A1.1\|X 3.42 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017318571.2	XP_017174060.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
NM_019582.2	NP_062528.2	voltage-dependent L-type calcium channel subunit alpha-1F
XM_011247482.2	XP_011245784.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
XM_017318571.1	XP_017174060.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
XM_030251408.1	XP_030107268.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1F	NP_005174.2
	CACNA1F	XP_001106163.2
Canis lupus familiaris (dog)	CACNA1F	XP_005642146.1
Danio rerio (zebrafish)	LOC559964	XP_003199134.2
Bos taurus (cattle)	CACNA1F	NP_001192592.1
Mus musculus (house mouse)	Cacna1f	NP_062528.2
Danio rerio (zebrafish)	LOC793708	XP_001333514.5
Xenopus tropicalis (tropical clawed frog)	cacna1f	XP_002941018.2
Rattus norvegicus (Norway rat)	Cacna1f	NP_446153.1

Gene Ontology
Bibliography

Related articles in PubMed

Cone dystrophy and ectopic synaptogenesis in a Cacna1f loss of function model of congenital stationary night blindness (CSNB2A).
Waldner DM, Giraldo Sierra NC, Bonfield S, Nguyen L, Dimopoulos IS, Sauv? Y, Stell WK, Bech-Hansen NT
Channels (Austin, Tex.)12(1)17-33(2018 Jan)

A missense mutation in
Peachey NS, Hasan N, FitzMaurice B, Burrill S, Pangeni G, Karst SY, Reinholdt L, Berry ML, Strobel M, Gregg RG, McCall MA, Chang B
Journal of neurophysiology118(2)845-854(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that genetic deletion of the synaptic Cav1.4 L-type voltage-gated calcium channels impairs calpain activation and leads to a short-term preservation of photoreceptors in the rd1 mouse.
Title: Cav1.4 L-Type Calcium Channels Contribute to Calpain Activation in Degenerating Photoreceptors of rd1 Mice.

analysis of Cav1.4 complexes alpha11.4, beta2, and alpha2delta4 in HEK293T cells and in mouse retina
Title: Characterization of Cav1.4 complexes (?11.4, ?2, and ?2?4) in HEK293T cells and in the retina.

CaBP4 forms a collapsed structure around the IQ motif in Cav1.4 that may promote channel activation by disrupting an interaction between IQ and the inhibitor of Ca(2+)-dependent inactivation domain
Title: Structural insights into activation of the retinal L-type Ca?? channel (Cav1.4) by Ca??-binding protein 4 (CaBP4).

Data on Cav1.4 deficient mice and human female carriers of mutations in CACNA1F are consistent with a phenotype of mosaic congenital stationary night blindness type 2A.
Title: Mosaic synaptopathy and functional defects in Cav1.4 heterozygous mice and human carriers of CSNB2.

the importance of proper Cav 1.4 function for efficient PR synapse maturation, and that dysregulation of Cav 1.4 channels in CSNB2 may have synaptopathic consequences
Title: Dysregulation of Ca(v)1.4 channels disrupts the maturation of photoreceptor synaptic ribbons in congenital stationary night blindness type 2.

The following Cacna1f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83164	XM_017318571.2 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu90428	XM_030251408.1 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu15776	NM_019582.2 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1462.30 $2089.00
OMu69566	XM_011247482.2 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu83164	XM_017318571.1	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1f ( XM_017318571.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174060.1 Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu83164
Clone ID Related Accession (Same CDS sequence)	XM_017318571.2 , XM_017318571.1
Accession Version	XM_017318571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCCC TTGGATCTCC CAGAAAACAC CAATCCTGGT TTCTACTCAC TGCCTTTTCT 
CCACTCCCCA GGCTTCGGAA GGCCTGCCAC ACACTCATAC ATCACCATAT CTTCACCAGT 
CTCATCCTAG TGTTCATCAT CCTCAGTAGT GTGTCCCTGG CTGCTGAGGA CCCCATCCGA 
GCTCACTCCT TCCGAAACCA TATTCTGGGA TATTTTGATT ATGCCTTCAC CTCCATATTC 
ACTGTGGAGA TTCTACTCAA GATGACAGTG TTTGGGGCCT TCCTGCACCG AGGCTCTTTC 
TGCCGTAGCT GGTTCAATCT GTTGGATCTC CTTGTGGTCA GTGTGTCCCT CATCTCCTTC 
GGCATCCACT CCAGTGCCAT CTCAGTTGTG AAGATTCTCC GAGTCCTCCG AGTCCTGCGG 
CCTCTCCGAG CCATCAACAG AGCCAAGGGA CTCAAGCATG TGGTGCAGTG TGTGTTCGTG 
GCCATCCGGA CCATCGGAAA CATCATGATT GTCACCACCC TCTTGCAGTT CATGTTCGCC 
TGCATTGGTG TTCAGCTGTT CAAGGGAAAA TTCTACAGTT GCACTGATGA GGCCAAACAC 
ACCCTGAAAG AATGCAAGGG CTCCTTCCTC ATCTACCCTG ATGGAGATGT GTCACGACCT 
TTGGTCCGGG AGCGGCTCTG GGTCAACAGT GATTTTAACT TTGACAACGT CCTTTCAGCC 
ATGATGGCCC TGTTCACTGT CTCTACCTTT GAAGGCTGGC CTGCGCTACT ATACAAGGCC 
ATAGATGCAA ACGCAGAAGA TGAGGGCCCT ATCTACAATT ACCATGTGGA GATATCAGTA 
TTCTTCATTG TCTACATCAT CATCATCGCC TTCTTCATGA TGAACATCTT TGTGGGCTTT 
GTTATCATCA CATTCCGTGC CCAGGGAGAG CAGGAGTACC AAAACTGTGA ACTGGACAAG 
AACCAGCGCC AGTGTGTGGA ATATGCCCTC AAAGCTCAGC CACTCCGCCG ATACATCCCT 
AAGAATCCTC ATCAGTACCG CGTGTGGGCC ACTGTGAACT CTGCTGCCTT TGAGTACCTC 
ATGTTTCTGC TCATCCTGCT CAACACGGTG GCCCTAGCCA TGCAGCACTA TGAACAGACT 
GCTCCCTTTA ACTATGCCAT GGACATCCTC AACATGGTCT TCACTGGCCT CTTCACCATT 
GAGATGGTGC TCAAAATCAT CGCCTTTAAA CCCAAGCATT ACTTTGCAGA TGCCTGGAAT 
ACGTTTGATG CTCTCATTGT AGTGGGCAGT GTAGTCGACA TCGCCGTCAC AGAAGTCAAT 
AGTTCAGAGG ACAGCTCCCG CATATCTATC ACGTTCTTTC GCCTCTTCCG AGTCATGAGG 
CTGGTCAAGC TTCTGAGTAA GGGTGAGGGG ATCCGCACAC TGCTCTGGAC ATTCATCAAG 
TCTTTCCAGG CCTTGCCCTA TGTGGCACTT CTCATAGCAA TGATATTCTT CATCTATGCA 
GTCATTGGCA TGCAGATGTT TGGCAAGGTG GCTCTTCAGG ACGGCACGCA GATAAATCGA 
AACAACAATT TCCAGACCTT TCCGCAGGCT GTGCTGCTTC TGTTCAGGTG TGCCACTGGT 
GAGGCCTGGC AAGAGATAAT GCTAGCCAGC CTTCCAGGAA ATCGATGTGA CCCTGAGTCT 
GACTTTGGCC CAGGCGAGGA ATTTACCTGT GGTAGCAGTT TTGCCATCGT CTACTTCATC 
AGCTTCTTTA TGCTCTGTGC CTTCCTGATT ATAAATCTCT TTGTGGCTGT AATCATGGAT 
AACTTTGATT ACCTAACCAG AGATTGGTCT ATCCTGGGAC CCCACCACCT TGATGAATTC 
AAGAGGATCT GGTCTGAATA TGACCCCGGA GCCAAGGGCC GCATCAAGCA CTTGGATGTG 
GTTGCCCTGC TGAGACGCAT CCAGCCCCCA TTGGGATTTG GAAAGCTATG CCCACACCGA 
GTGGCCTGCA AGAGACTCGT GGCAATGAAT GTGCCCCTCA ACTCAGATGG AACAGTGACA 
TTCAACGCTA CACTCTTTGC CCTGGTGCGG ACATCCCTGA AGATCAAGAC AGAAGGGAAC 
CTGGATCAAG CCAACCAGGA GCTTCGGATG GTCATCAAAA AGATCTGGAA GCGGATAAAG 
CAGAAATTGT TGGATGAGGT CATCCCTCCT CCCGATGAGG AGGAGGTCAC TGTGGGAAAA 
TTCTATGCCA CATTCCTGAT CCAAGATTAT TTCCGAAAAT TCCGGAGAAG GAAAGAAAAG 
GGGCTACTAG GAAGAGAGGC CCCAACAAGC ACATCCTCTG CCCTCCAGGC TGGTCTAAGG 
AGCCTGCAGG ACTTGGGTCC TGAGATCCGT CAAGCCCTCA CCTATGACAC TGAGGAAGAA 
GAGGAAGAGG AAGAGGCAGT GGGTCAGGAG GCTGAGGAAG AGGAAGCTGA GAACAACCCA 
GAACCATACA AAGACTCCAT AGACTCCCAG CCCCAATCTC GATGGAACTC TAGGATTTCG 
GTGTCTCTAC CTGTTAAGGA GAAACTTCCA GATTCTCTCT CAACTGGGCC GAGTGATGAT 
GATGGGCTGG CTCCCAACTC CAGGCAGCCC AGTGTGATAC AGGCTGGCTC CCAACCACAC 
AGGAGAAGCT CTGGGGTTTT CATGTTCACT ATCCCGGAAG AAGGAAGTAT TCAGCTCAAG 
GGAACTCAAG GGCAGGACAA TCAGAATGAG GAACAGGAAG TCCCTGACTG GACTCCTGAC 
CTGGATGAGC AGGCCGGGAC TCCTTCGAAC CCAGTCCTTT TACCACCTCA CTGGTCCCAG 
CAACACGTAA ACGGGCACCA TGTGCCACGC CGACGTTTGC TGCCCCCCAC GCCTGCAGGT 
CGGAAGCCCT CCTTCACCAT CCAGTGTCTG CAACGCCAGG GCAGTTGTGA AGATTTACCT 
ATCCCAGGCA CCTACCATCG TGGACGGACC TCAGGACCAA GCAGGGCTCA GGGTTCCTGG 
GCAGCCCCTC CTCAGAAGGG TCGACTGCTA TATGCCCCCC TGTTGTTGGT GGAGGAATCT 
ACAGTGGGTG AAGGATACCT TGGCAAACTT GGCGGCCCAC TGCGTACCTT CACCTGTCTG 
CAAGTGCCTG GAGCTCATCC GAATCCCAGC CACCGCAAGA GGGGCAGTGC TGACAGTTTG 
GTGGAGGCTG TGCTCATCTC CGAAGGCCTA GGTCTCTTTG CCCAAGACCC ACGATTTGTG 
GCCCTGGCCA AGCAGGAGAT TGCAGATGCA TGTCACCTGA CCCTGGATGA GATGGACAGT 
GCTGCCAGTG ACCTGCTGGC ACAGAGAACC ACCTCCCTTT ACAGTGATGA GGAGTCTATT 
CTTTCCCGCT TTGATGAAGA GGACCTGGGA GATGAGATGG CCTGTGTCCA TGCCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174060.1
CDS	260..3679
Translation	MYPLGSPRKHQSWFLLTAFSPLPRLRKACHTLIHHHIFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNSSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSSFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLKIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGREAPTSTSSALQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAVGQEAEEEEAENNPEPYKDSIDSQPQSRWNSRISVSLPVKEKLPDSLSTGPSDDDGLAPNSRQPSVIQAGSQPHRRSSGVFMFTIPEEGSIQLKGTQGQDNQNEEQEVPDWTPDLDEQAGTPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPSRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAHPNPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_017318571.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318571.2

ORF Insert Sequence:

ATGTATCCCC TTGGATCTCC CAGAAAACAC CAATCCTGGT TTCTACTCAC TGCCTTTTCT 
CCACTCCCCA GGCTTCGGAA GGCCTGCCAC ACACTCATAC ATCACCATAT CTTCACCAGT 
CTCATCCTAG TGTTCATCAT CCTCAGTAGT GTGTCCCTGG CTGCTGAGGA CCCCATCCGA 
GCTCACTCCT TCCGAAACCA TATTCTGGGA TATTTTGATT ATGCCTTCAC CTCCATATTC 
ACTGTGGAGA TTCTACTCAA GATGACAGTG TTTGGGGCCT TCCTGCACCG AGGCTCTTTC 
TGCCGTAGCT GGTTCAATCT GTTGGATCTC CTTGTGGTCA GTGTGTCCCT CATCTCCTTC 
GGCATCCACT CCAGTGCCAT CTCAGTTGTG AAGATTCTCC GAGTCCTCCG AGTCCTGCGG 
CCTCTCCGAG CCATCAACAG AGCCAAGGGA CTCAAGCATG TGGTGCAGTG TGTGTTCGTG 
GCCATCCGGA CCATCGGAAA CATCATGATT GTCACCACCC TCTTGCAGTT CATGTTCGCC 
TGCATTGGTG TTCAGCTGTT CAAGGGAAAA TTCTACAGTT GCACTGATGA GGCCAAACAC 
ACCCTGAAAG AATGCAAGGG CTCCTTCCTC ATCTACCCTG ATGGAGATGT GTCACGACCT 
TTGGTCCGGG AGCGGCTCTG GGTCAACAGT GATTTTAACT TTGACAACGT CCTTTCAGCC 
ATGATGGCCC TGTTCACTGT CTCTACCTTT GAAGGCTGGC CTGCGCTACT ATACAAGGCC 
ATAGATGCAA ACGCAGAAGA TGAGGGCCCT ATCTACAATT ACCATGTGGA GATATCAGTA 
TTCTTCATTG TCTACATCAT CATCATCGCC TTCTTCATGA TGAACATCTT TGTGGGCTTT 
GTTATCATCA CATTCCGTGC CCAGGGAGAG CAGGAGTACC AAAACTGTGA ACTGGACAAG 
AACCAGCGCC AGTGTGTGGA ATATGCCCTC AAAGCTCAGC CACTCCGCCG ATACATCCCT 
AAGAATCCTC ATCAGTACCG CGTGTGGGCC ACTGTGAACT CTGCTGCCTT TGAGTACCTC 
ATGTTTCTGC TCATCCTGCT CAACACGGTG GCCCTAGCCA TGCAGCACTA TGAACAGACT 
GCTCCCTTTA ACTATGCCAT GGACATCCTC AACATGGTCT TCACTGGCCT CTTCACCATT 
GAGATGGTGC TCAAAATCAT CGCCTTTAAA CCCAAGCATT ACTTTGCAGA TGCCTGGAAT 
ACGTTTGATG CTCTCATTGT AGTGGGCAGT GTAGTCGACA TCGCCGTCAC AGAAGTCAAT 
AGTTCAGAGG ACAGCTCCCG CATATCTATC ACGTTCTTTC GCCTCTTCCG AGTCATGAGG 
CTGGTCAAGC TTCTGAGTAA GGGTGAGGGG ATCCGCACAC TGCTCTGGAC ATTCATCAAG 
TCTTTCCAGG CCTTGCCCTA TGTGGCACTT CTCATAGCAA TGATATTCTT CATCTATGCA 
GTCATTGGCA TGCAGATGTT TGGCAAGGTG GCTCTTCAGG ACGGCACGCA GATAAATCGA 
AACAACAATT TCCAGACCTT TCCGCAGGCT GTGCTGCTTC TGTTCAGGTG TGCCACTGGT 
GAGGCCTGGC AAGAGATAAT GCTAGCCAGC CTTCCAGGAA ATCGATGTGA CCCTGAGTCT 
GACTTTGGCC CAGGCGAGGA ATTTACCTGT GGTAGCAGTT TTGCCATCGT CTACTTCATC 
AGCTTCTTTA TGCTCTGTGC CTTCCTGATT ATAAATCTCT TTGTGGCTGT AATCATGGAT 
AACTTTGATT ACCTAACCAG AGATTGGTCT ATCCTGGGAC CCCACCACCT TGATGAATTC 
AAGAGGATCT GGTCTGAATA TGACCCCGGA GCCAAGGGCC GCATCAAGCA CTTGGATGTG 
GTTGCCCTGC TGAGACGCAT CCAGCCCCCA TTGGGATTTG GAAAGCTATG CCCACACCGA 
GTGGCCTGCA AGAGACTCGT GGCAATGAAT GTGCCCCTCA ACTCAGATGG AACAGTGACA 
TTCAACGCTA CACTCTTTGC CCTGGTGCGG ACATCCCTGA AGATCAAGAC AGAAGGGAAC 
CTGGATCAAG CCAACCAGGA GCTTCGGATG GTCATCAAAA AGATCTGGAA GCGGATAAAG 
CAGAAATTGT TGGATGAGGT CATCCCTCCT CCCGATGAGG AGGAGGTCAC TGTGGGAAAA 
TTCTATGCCA CATTCCTGAT CCAAGATTAT TTCCGAAAAT TCCGGAGAAG GAAAGAAAAG 
GGGCTACTAG GAAGAGAGGC CCCAACAAGC ACATCCTCTG CCCTCCAGGC TGGTCTAAGG 
AGCCTGCAGG ACTTGGGTCC TGAGATCCGT CAAGCCCTCA CCTATGACAC TGAGGAAGAA 
GAGGAAGAGG AAGAGGCAGT GGGTCAGGAG GCTGAGGAAG AGGAAGCTGA GAACAACCCA 
GAACCATACA AAGACTCCAT AGACTCCCAG CCCCAATCTC GATGGAACTC TAGGATTTCG 
GTGTCTCTAC CTGTTAAGGA GAAACTTCCA GATTCTCTCT CAACTGGGCC GAGTGATGAT 
GATGGGCTGG CTCCCAACTC CAGGCAGCCC AGTGTGATAC AGGCTGGCTC CCAACCACAC 
AGGAGAAGCT CTGGGGTTTT CATGTTCACT ATCCCGGAAG AAGGAAGTAT TCAGCTCAAG 
GGAACTCAAG GGCAGGACAA TCAGAATGAG GAACAGGAAG TCCCTGACTG GACTCCTGAC 
CTGGATGAGC AGGCCGGGAC TCCTTCGAAC CCAGTCCTTT TACCACCTCA CTGGTCCCAG 
CAACACGTAA ACGGGCACCA TGTGCCACGC CGACGTTTGC TGCCCCCCAC GCCTGCAGGT 
CGGAAGCCCT CCTTCACCAT CCAGTGTCTG CAACGCCAGG GCAGTTGTGA AGATTTACCT 
ATCCCAGGCA CCTACCATCG TGGACGGACC TCAGGACCAA GCAGGGCTCA GGGTTCCTGG 
GCAGCCCCTC CTCAGAAGGG TCGACTGCTA TATGCCCCCC TGTTGTTGGT GGAGGAATCT 
ACAGTGGGTG AAGGATACCT TGGCAAACTT GGCGGCCCAC TGCGTACCTT CACCTGTCTG 
CAAGTGCCTG GAGCTCATCC GAATCCCAGC CACCGCAAGA GGGGCAGTGC TGACAGTTTG 
GTGGAGGCTG TGCTCATCTC CGAAGGCCTA GGTCTCTTTG CCCAAGACCC ACGATTTGTG 
GCCCTGGCCA AGCAGGAGAT TGCAGATGCA TGTCACCTGA CCCTGGATGA GATGGACAGT 
GCTGCCAGTG ACCTGCTGGC ACAGAGAACC ACCTCCCTTT ACAGTGATGA GGAGTCTATT 
CTTTCCCGCT TTGATGAAGA GGACCTGGGA GATGAGATGG CCTGTGTCCA TGCCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1f ( XM_017318571.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174060.1 Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu83164
Clone ID Related Accession (Same CDS sequence)	XM_017318571.2 , XM_017318571.1
Accession Version	XM_017318571.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCCC TTGGATCTCC CAGAAAACAC CAATCCTGGT TTCTACTCAC TGCCTTTTCT 
CCACTCCCCA GGCTTCGGAA GGCCTGCCAC ACACTCATAC ATCACCATAT CTTCACCAGT 
CTCATCCTAG TGTTCATCAT CCTCAGTAGT GTGTCCCTGG CTGCTGAGGA CCCCATCCGA 
GCTCACTCCT TCCGAAACCA TATTCTGGGA TATTTTGATT ATGCCTTCAC CTCCATATTC 
ACTGTGGAGA TTCTACTCAA GATGACAGTG TTTGGGGCCT TCCTGCACCG AGGCTCTTTC 
TGCCGTAGCT GGTTCAATCT GTTGGATCTC CTTGTGGTCA GTGTGTCCCT CATCTCCTTC 
GGCATCCACT CCAGTGCCAT CTCAGTTGTG AAGATTCTCC GAGTCCTCCG AGTCCTGCGG 
CCTCTCCGAG CCATCAACAG AGCCAAGGGA CTCAAGCATG TGGTGCAGTG TGTGTTCGTG 
GCCATCCGGA CCATCGGAAA CATCATGATT GTCACCACCC TCTTGCAGTT CATGTTCGCC 
TGCATTGGTG TTCAGCTGTT CAAGGGAAAA TTCTACAGTT GCACTGATGA GGCCAAACAC 
ACCCTGAAAG AATGCAAGGG CTCCTTCCTC ATCTACCCTG ATGGAGATGT GTCACGACCT 
TTGGTCCGGG AGCGGCTCTG GGTCAACAGT GATTTTAACT TTGACAACGT CCTTTCAGCC 
ATGATGGCCC TGTTCACTGT CTCTACCTTT GAAGGCTGGC CTGCGCTACT ATACAAGGCC 
ATAGATGCAA ACGCAGAAGA TGAGGGCCCT ATCTACAATT ACCATGTGGA GATATCAGTA 
TTCTTCATTG TCTACATCAT CATCATCGCC TTCTTCATGA TGAACATCTT TGTGGGCTTT 
GTTATCATCA CATTCCGTGC CCAGGGAGAG CAGGAGTACC AAAACTGTGA ACTGGACAAG 
AACCAGCGCC AGTGTGTGGA ATATGCCCTC AAAGCTCAGC CACTCCGCCG ATACATCCCT 
AAGAATCCTC ATCAGTACCG CGTGTGGGCC ACTGTGAACT CTGCTGCCTT TGAGTACCTC 
ATGTTTCTGC TCATCCTGCT CAACACGGTG GCCCTAGCCA TGCAGCACTA TGAACAGACT 
GCTCCCTTTA ACTATGCCAT GGACATCCTC AACATGGTCT TCACTGGCCT CTTCACCATT 
GAGATGGTGC TCAAAATCAT CGCCTTTAAA CCCAAGCATT ACTTTGCAGA TGCCTGGAAT 
ACGTTTGATG CTCTCATTGT AGTGGGCAGT GTAGTCGACA TCGCCGTCAC AGAAGTCAAT 
AGTTCAGAGG ACAGCTCCCG CATATCTATC ACGTTCTTTC GCCTCTTCCG AGTCATGAGG 
CTGGTCAAGC TTCTGAGTAA GGGTGAGGGG ATCCGCACAC TGCTCTGGAC ATTCATCAAG 
TCTTTCCAGG CCTTGCCCTA TGTGGCACTT CTCATAGCAA TGATATTCTT CATCTATGCA 
GTCATTGGCA TGCAGATGTT TGGCAAGGTG GCTCTTCAGG ACGGCACGCA GATAAATCGA 
AACAACAATT TCCAGACCTT TCCGCAGGCT GTGCTGCTTC TGTTCAGGTG TGCCACTGGT 
GAGGCCTGGC AAGAGATAAT GCTAGCCAGC CTTCCAGGAA ATCGATGTGA CCCTGAGTCT 
GACTTTGGCC CAGGCGAGGA ATTTACCTGT GGTAGCAGTT TTGCCATCGT CTACTTCATC 
AGCTTCTTTA TGCTCTGTGC CTTCCTGATT ATAAATCTCT TTGTGGCTGT AATCATGGAT 
AACTTTGATT ACCTAACCAG AGATTGGTCT ATCCTGGGAC CCCACCACCT TGATGAATTC 
AAGAGGATCT GGTCTGAATA TGACCCCGGA GCCAAGGGCC GCATCAAGCA CTTGGATGTG 
GTTGCCCTGC TGAGACGCAT CCAGCCCCCA TTGGGATTTG GAAAGCTATG CCCACACCGA 
GTGGCCTGCA AGAGACTCGT GGCAATGAAT GTGCCCCTCA ACTCAGATGG AACAGTGACA 
TTCAACGCTA CACTCTTTGC CCTGGTGCGG ACATCCCTGA AGATCAAGAC AGAAGGGAAC 
CTGGATCAAG CCAACCAGGA GCTTCGGATG GTCATCAAAA AGATCTGGAA GCGGATAAAG 
CAGAAATTGT TGGATGAGGT CATCCCTCCT CCCGATGAGG AGGAGGTCAC TGTGGGAAAA 
TTCTATGCCA CATTCCTGAT CCAAGATTAT TTCCGAAAAT TCCGGAGAAG GAAAGAAAAG 
GGGCTACTAG GAAGAGAGGC CCCAACAAGC ACATCCTCTG CCCTCCAGGC TGGTCTAAGG 
AGCCTGCAGG ACTTGGGTCC TGAGATCCGT CAAGCCCTCA CCTATGACAC TGAGGAAGAA 
GAGGAAGAGG AAGAGGCAGT GGGTCAGGAG GCTGAGGAAG AGGAAGCTGA GAACAACCCA 
GAACCATACA AAGACTCCAT AGACTCCCAG CCCCAATCTC GATGGAACTC TAGGATTTCG 
GTGTCTCTAC CTGTTAAGGA GAAACTTCCA GATTCTCTCT CAACTGGGCC GAGTGATGAT 
GATGGGCTGG CTCCCAACTC CAGGCAGCCC AGTGTGATAC AGGCTGGCTC CCAACCACAC 
AGGAGAAGCT CTGGGGTTTT CATGTTCACT ATCCCGGAAG AAGGAAGTAT TCAGCTCAAG 
GGAACTCAAG GGCAGGACAA TCAGAATGAG GAACAGGAAG TCCCTGACTG GACTCCTGAC 
CTGGATGAGC AGGCCGGGAC TCCTTCGAAC CCAGTCCTTT TACCACCTCA CTGGTCCCAG 
CAACACGTAA ACGGGCACCA TGTGCCACGC CGACGTTTGC TGCCCCCCAC GCCTGCAGGT 
CGGAAGCCCT CCTTCACCAT CCAGTGTCTG CAACGCCAGG GCAGTTGTGA AGATTTACCT 
ATCCCAGGCA CCTACCATCG TGGACGGACC TCAGGACCAA GCAGGGCTCA GGGTTCCTGG 
GCAGCCCCTC CTCAGAAGGG TCGACTGCTA TATGCCCCCC TGTTGTTGGT GGAGGAATCT 
ACAGTGGGTG AAGGATACCT TGGCAAACTT GGCGGCCCAC TGCGTACCTT CACCTGTCTG 
CAAGTGCCTG GAGCTCATCC GAATCCCAGC CACCGCAAGA GGGGCAGTGC TGACAGTTTG 
GTGGAGGCTG TGCTCATCTC CGAAGGCCTA GGTCTCTTTG CCCAAGACCC ACGATTTGTG 
GCCCTGGCCA AGCAGGAGAT TGCAGATGCA TGTCACCTGA CCCTGGATGA GATGGACAGT 
GCTGCCAGTG ACCTGCTGGC ACAGAGAACC ACCTCCCTTT ACAGTGATGA GGAGTCTATT 
CTTTCCCGCT TTGATGAAGA GGACCTGGGA GATGAGATGG CCTGTGTCCA TGCCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174060.1
CDS	899..4318
Translation	MYPLGSPRKHQSWFLLTAFSPLPRLRKACHTLIHHHIFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNSSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSSFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLKIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGREAPTSTSSALQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAVGQEAEEEEAENNPEPYKDSIDSQPQSRWNSRISVSLPVKEKLPDSLSTGPSDDDGLAPNSRQPSVIQAGSQPHRRSSGVFMFTIPEEGSIQLKGTQGQDNQNEEQEVPDWTPDLDEQAGTPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPSRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAHPNPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_017318571.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318571.1

ORF Insert Sequence:

ATGTATCCCC TTGGATCTCC CAGAAAACAC CAATCCTGGT TTCTACTCAC TGCCTTTTCT 
CCACTCCCCA GGCTTCGGAA GGCCTGCCAC ACACTCATAC ATCACCATAT CTTCACCAGT 
CTCATCCTAG TGTTCATCAT CCTCAGTAGT GTGTCCCTGG CTGCTGAGGA CCCCATCCGA 
GCTCACTCCT TCCGAAACCA TATTCTGGGA TATTTTGATT ATGCCTTCAC CTCCATATTC 
ACTGTGGAGA TTCTACTCAA GATGACAGTG TTTGGGGCCT TCCTGCACCG AGGCTCTTTC 
TGCCGTAGCT GGTTCAATCT GTTGGATCTC CTTGTGGTCA GTGTGTCCCT CATCTCCTTC 
GGCATCCACT CCAGTGCCAT CTCAGTTGTG AAGATTCTCC GAGTCCTCCG AGTCCTGCGG 
CCTCTCCGAG CCATCAACAG AGCCAAGGGA CTCAAGCATG TGGTGCAGTG TGTGTTCGTG 
GCCATCCGGA CCATCGGAAA CATCATGATT GTCACCACCC TCTTGCAGTT CATGTTCGCC 
TGCATTGGTG TTCAGCTGTT CAAGGGAAAA TTCTACAGTT GCACTGATGA GGCCAAACAC 
ACCCTGAAAG AATGCAAGGG CTCCTTCCTC ATCTACCCTG ATGGAGATGT GTCACGACCT 
TTGGTCCGGG AGCGGCTCTG GGTCAACAGT GATTTTAACT TTGACAACGT CCTTTCAGCC 
ATGATGGCCC TGTTCACTGT CTCTACCTTT GAAGGCTGGC CTGCGCTACT ATACAAGGCC 
ATAGATGCAA ACGCAGAAGA TGAGGGCCCT ATCTACAATT ACCATGTGGA GATATCAGTA 
TTCTTCATTG TCTACATCAT CATCATCGCC TTCTTCATGA TGAACATCTT TGTGGGCTTT 
GTTATCATCA CATTCCGTGC CCAGGGAGAG CAGGAGTACC AAAACTGTGA ACTGGACAAG 
AACCAGCGCC AGTGTGTGGA ATATGCCCTC AAAGCTCAGC CACTCCGCCG ATACATCCCT 
AAGAATCCTC ATCAGTACCG CGTGTGGGCC ACTGTGAACT CTGCTGCCTT TGAGTACCTC 
ATGTTTCTGC TCATCCTGCT CAACACGGTG GCCCTAGCCA TGCAGCACTA TGAACAGACT 
GCTCCCTTTA ACTATGCCAT GGACATCCTC AACATGGTCT TCACTGGCCT CTTCACCATT 
GAGATGGTGC TCAAAATCAT CGCCTTTAAA CCCAAGCATT ACTTTGCAGA TGCCTGGAAT 
ACGTTTGATG CTCTCATTGT AGTGGGCAGT GTAGTCGACA TCGCCGTCAC AGAAGTCAAT 
AGTTCAGAGG ACAGCTCCCG CATATCTATC ACGTTCTTTC GCCTCTTCCG AGTCATGAGG 
CTGGTCAAGC TTCTGAGTAA GGGTGAGGGG ATCCGCACAC TGCTCTGGAC ATTCATCAAG 
TCTTTCCAGG CCTTGCCCTA TGTGGCACTT CTCATAGCAA TGATATTCTT CATCTATGCA 
GTCATTGGCA TGCAGATGTT TGGCAAGGTG GCTCTTCAGG ACGGCACGCA GATAAATCGA 
AACAACAATT TCCAGACCTT TCCGCAGGCT GTGCTGCTTC TGTTCAGGTG TGCCACTGGT 
GAGGCCTGGC AAGAGATAAT GCTAGCCAGC CTTCCAGGAA ATCGATGTGA CCCTGAGTCT 
GACTTTGGCC CAGGCGAGGA ATTTACCTGT GGTAGCAGTT TTGCCATCGT CTACTTCATC 
AGCTTCTTTA TGCTCTGTGC CTTCCTGATT ATAAATCTCT TTGTGGCTGT AATCATGGAT 
AACTTTGATT ACCTAACCAG AGATTGGTCT ATCCTGGGAC CCCACCACCT TGATGAATTC 
AAGAGGATCT GGTCTGAATA TGACCCCGGA GCCAAGGGCC GCATCAAGCA CTTGGATGTG 
GTTGCCCTGC TGAGACGCAT CCAGCCCCCA TTGGGATTTG GAAAGCTATG CCCACACCGA 
GTGGCCTGCA AGAGACTCGT GGCAATGAAT GTGCCCCTCA ACTCAGATGG AACAGTGACA 
TTCAACGCTA CACTCTTTGC CCTGGTGCGG ACATCCCTGA AGATCAAGAC AGAAGGGAAC 
CTGGATCAAG CCAACCAGGA GCTTCGGATG GTCATCAAAA AGATCTGGAA GCGGATAAAG 
CAGAAATTGT TGGATGAGGT CATCCCTCCT CCCGATGAGG AGGAGGTCAC TGTGGGAAAA 
TTCTATGCCA CATTCCTGAT CCAAGATTAT TTCCGAAAAT TCCGGAGAAG GAAAGAAAAG 
GGGCTACTAG GAAGAGAGGC CCCAACAAGC ACATCCTCTG CCCTCCAGGC TGGTCTAAGG 
AGCCTGCAGG ACTTGGGTCC TGAGATCCGT CAAGCCCTCA CCTATGACAC TGAGGAAGAA 
GAGGAAGAGG AAGAGGCAGT GGGTCAGGAG GCTGAGGAAG AGGAAGCTGA GAACAACCCA 
GAACCATACA AAGACTCCAT AGACTCCCAG CCCCAATCTC GATGGAACTC TAGGATTTCG 
GTGTCTCTAC CTGTTAAGGA GAAACTTCCA GATTCTCTCT CAACTGGGCC GAGTGATGAT 
GATGGGCTGG CTCCCAACTC CAGGCAGCCC AGTGTGATAC AGGCTGGCTC CCAACCACAC 
AGGAGAAGCT CTGGGGTTTT CATGTTCACT ATCCCGGAAG AAGGAAGTAT TCAGCTCAAG 
GGAACTCAAG GGCAGGACAA TCAGAATGAG GAACAGGAAG TCCCTGACTG GACTCCTGAC 
CTGGATGAGC AGGCCGGGAC TCCTTCGAAC CCAGTCCTTT TACCACCTCA CTGGTCCCAG 
CAACACGTAA ACGGGCACCA TGTGCCACGC CGACGTTTGC TGCCCCCCAC GCCTGCAGGT 
CGGAAGCCCT CCTTCACCAT CCAGTGTCTG CAACGCCAGG GCAGTTGTGA AGATTTACCT 
ATCCCAGGCA CCTACCATCG TGGACGGACC TCAGGACCAA GCAGGGCTCA GGGTTCCTGG 
GCAGCCCCTC CTCAGAAGGG TCGACTGCTA TATGCCCCCC TGTTGTTGGT GGAGGAATCT 
ACAGTGGGTG AAGGATACCT TGGCAAACTT GGCGGCCCAC TGCGTACCTT CACCTGTCTG 
CAAGTGCCTG GAGCTCATCC GAATCCCAGC CACCGCAAGA GGGGCAGTGC TGACAGTTTG 
GTGGAGGCTG TGCTCATCTC CGAAGGCCTA GGTCTCTTTG CCCAAGACCC ACGATTTGTG 
GCCCTGGCCA AGCAGGAGAT TGCAGATGCA TGTCACCTGA CCCTGGATGA GATGGACAGT 
GCTGCCAGTG ACCTGCTGGC ACAGAGAACC ACCTCCCTTT ACAGTGATGA GGAGTCTATT 
CTTTCCCGCT TTGATGAAGA GGACCTGGGA GATGAGATGG CCTGTGTCCA TGCCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1f ( XM_030251408.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107268.1 Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X2, mRNA.

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CloneID	OMu90428
Clone ID Related Accession (Same CDS sequence)	XM_030251408.1
Accession Version	XM_030251408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAT CTGAAGTCGG GAAAGGTGAG AGAAGCCATC CACCCCTGAA GACCCTTGGC 
ATCAGCATTG TAGAGTGGAA GCCTTTTGAT ATTCTCATCC TCCTGACAAT CTTTGCCAAC 
TGCGTGGCAT TGGGGGTATA TATCCCCTTC CCTGAGGACG ACTCCAACAC TGCTAACCAC 
AACTTGGAAC AGGTAGAATA CGTGTTCCTG GTGATTTTCA CCGTGGAGAC AGTGCTCAAG 
ATCGTAGCCT ATGGGCTGGT GCTCCATCCC AGCGCCTATA TTCGCAATGG CTGGAACCTG 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTGC TGCTGGAACA AGGACCTGGG 
CGGCCAGGAG ATGCCCCGCA TACTGGAGGA AAGCCAGGAG GCTTCGATGT AAAGGCACTG 
CGGGCATTTA GGGTGCTACG ACCTCTAAGG CTAGTGTCTG GGGTCCCGAG TCTGCACATA 
GTGCTCAATT CCATCATGAA GGCGCTTGTG CCGCTGCTGC ACATTGCCCT GTTGGTGCTC 
TTCGTCATTA TCATTTACGC CATCATCGGA CTCGAGCTAT TCCTCGGACG AATGCACAAG 
ACATGCTACT TCCTGGGATC TGATATGGAA GCAGAGGAGG ACCCATCACC TTGTGCATCT 
TCTGGCTCTG GGCGTTCATG CACACTGAAC CATACCGAGT GCCGCGGGCG CTGGCCAGGA 
CCCAACGGTG GCATCACGAA CTTCGACAAT TTTTTCTTTG CCATGCTAAC TGTGTTCCAG 
TGTATTACCA TGGAAGGCTG GACAGACGTC CTCTACTGGA TGCAGGATGC CATGGGGTAT 
GAGCTGCCTT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT TGTCCTCAAC 
CTTGTGCTTG GAGTCCTAAG CGGGGAGTTC TCCAAGGAAA GAGAAAAGGC AAAAGCACGA 
GGTGACTTTC AGAAGCTTCG GGAGAAGCAG CAGATGGAAG AAGACCTTCG GGGCTACCTG 
GACTGGATCA CACAGGCTGA GGAGTTAGAC CTTCATGACC CCTCAGTAGA CGGCAACTTG 
GCTTCTCTTG CTGAAGAGGG ACGGGCGGGC CATCGGCCAC AACTGTCAGA GCTGACCAAT 
AGGAGGCGCG GACGGCTGCG ATGGTTCAGC CACTCTACTC GCTCCACACA CTCCACCAGC 
AGCCACGCCA GCCTCCCAGC CAGTGACACT GGCTCCATGA CAGACACCCC TGGAGATGAG 
GATGAAGAAG AGGGGACCAT GGCTAGCTGT ACACGCTGCC TAAACAAGAT TATGAAAACA 
AGGATCTGCC GCCACTTCCG CCGAGCCAAC CGGGGTCTCC GTGCACGCTG CCGCCGGGCC 
GTCAAGTCCA ACGCCTGCTA CTGGGCTGTA CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATAGCTTCAG AGCACCATGG GCAGCCTTTG TGGCTCACCC AGACCCAAGA GTATGCCAAC 
AAAGTTCTGC TCTGCCTCTT CACTGTGGAG ATGCTCCTCA AACTGTACGG CCTGGGCCCC 
TCTGTCTACG TTGCCTCCTT TTTCAACCGC TTTGACTGCT TCGTGGTCTG TGGGGGCATC 
CTAGAAACCA CTTTGGTGGA GGTGGGGGCC ATGCAGCCTC TTGGCATCTC AGTGCTCCGA 
TGTGTACGTC TCCTCAGGAT CTTCAAGGTC ACCAGGCACT GGGCATCCCT GAGCAATCTG 
GTGGCATCTT TGCTCAATTC CATGAAGTCC ATCGCCTCCT TGCTGCTTCT CCTCTTTCTC 
TTCATCATCA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGAGGAG CACCTTTGAT ACCTTCCCCC AAGCCCTCCT CACTGTCTTT 
CAGATCCTGA CTGGTGAGGA TTGGAACGTT GTCATGTATG ATGGTATCAT GGCCTACGGT 
GGGCCCTTCT TCCCAGGGAT GCTGGTGTGT GTTTATTTCA TCATCCTCTT CATCTGTGGC 
AACTACATCC TGCTGAACGT GTTTCTTGCC ATTGCCGTGG ATAACCTAGC CAGCGGGGAT 
GCAGGCACTG CCAAAGACAA GGGCAGAGAG AAGAGCAGTG AAGGAAACCC TCCAAAGGAG 
AACAAAGTAT TGGTGCCTGG TGGAGAGAAT GAGGACGCAA AGGGTGCAAG AAGTGAAGGA 
GCAGGCATGG AGGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGA GGAAGAGGAA 
AATGGTGCAG GACATGTGGA ACTCTTGCAG GAAGTAGTAC CCAAGGAGAA GGTGGTACCC 
ATCCCTGAAG GCAGTGCCTT CTTCTGCCTT AGCCAAACCA ACCCGCTTCG GAAGGCCTGC 
CACACACTCA TACATCACCA TATCTTCACC AGTCTCATCC TAGTGTTCAT CATCCTCAGT 
AGTGTGTCCC TGGCTGCTGA GGACCCCATC CGAGCTCACT CCTTCCGAAA CCATATTCTG 
GGATATTTTG ATTATGCCTT CACCTCCATA TTCACTGTGG AGATTCTACT CAAGATGACA 
GTGTTTGGGG CCTTCCTGCA CCGAGGCTCT TTCTGCCGTA GCTGGTTCAA TCTGTTGGAT 
CTCCTTGTGG TCAGTGTGTC CCTCATCTCC TTCGGCATCC ACTCCAGTGC CATCTCAGTT 
GTGAAGATTC TCCGAGTCCT CCGAGTCCTG CGGCCTCTCC GAGCCATCAA CAGAGCCAAG 
GGACTCAAGC ATGTGGTGCA GTGTGTGTTC GTGGCCATCC GGACCATCGG AAACATCATG 
ATTGTCACCA CCCTCTTGCA GTTCATGTTC GCCTGCATTG GTGTTCAGCT GTTCAAGGGA 
AAATTCTACA GTTGCACTGA TGAGGCCAAA CACACCCTGA AAGAATGCAA GGGCTCCTTC 
CTCATCTACC CTGATGGAGA TGTGTCACGA CCTTTGGTCC GGGAGCGGCT CTGGGTCAAC 
AGTGATTTTA ACTTTGACAA CGTCCTTTCA GCCATGATGG CCCTGTTCAC TGTCTCTACC 
TTTGAAGGCT GGCCTGCGCT ACTATACAAG GCCATAGATG CAAACGCAGA AGATGAGGGC 
CCTATCTACA ATTACCATGT GGAGATATCA GTATTCTTCA TTGTCTACAT CATCATCATC 
GCCTTCTTCA TGATGAACAT CTTTGTGGGC TTTGTTATCA TCACATTCCG TGCCCAGGGA 
GAGCAGGAGT ACCAAAACTG TGAACTGGAC AAGAACCAGC GCCAGTGTGT GGAATATGCC 
CTCAAAGCTC AGCCACTCCG CCGATACATC CCTAAGAATC CTCATCAGTA CCGCGTGTGG 
GCCACTGTGA ACTCTGCTGC CTTTGAGTAC CTCATGTTTC TGCTCATCCT GCTCAACACG 
GTGGCCCTAG CCATGCAGCA CTATGAACAG ACTGCTCCCT TTAACTATGC CATGGACATC 
CTCAACATGG TCTTCACTGG CCTCTTCACC ATTGAGATGG TGCTCAAAAT CATCGCCTTT 
AAACCCAAGC ATTACTTTGC AGATGCCTGG AATACGTTTG ATGCTCTCAT TGTAGTGGGC 
AGTGTAGTCG ACATCGCCGT CACAGAAGTC AATGTGAGGA CTGGGCTTTG TGCTAACCCC 
TCTATCCTCT CCCTAGAGCC TTCTTTCACT TGGAGTTCAG AGGACAGCTC CCGCATATCT 
ATCACGTTCT TTCGCCTCTT CCGAGTCATG AGGCTGGTCA AGCTTCTGAG TAAGGGTGAG 
GGGATCCGCA CACTGCTCTG GACATTCATC AAGTCTTTCC AGGCCTTGCC CTATGTGGCA 
CTTCTCATAG CAATGATATT CTTCATCTAT GCAGTCATTG GCATGCAGAT GTTTGGCAAG 
GTGGCTCTTC AGGACGGCAC GCAGATAAAT CGAAACAACA ATTTCCAGAC CTTTCCGCAG 
GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCCT GGCAAGAGAT AATGCTAGCC 
AGCCTTCCAG GAAATCGATG TGACCCTGAG TCTGACTTTG GCCCAGGCGA GGAATTTACC 
TGTGGTAGCA GTTTTGCCAT CGTCTACTTC ATCAGCTTCT TTATGCTCTG TGCCTTCCTG 
ATTATAAATC TCTTTGTGGC TGTAATCATG GATAACTTTG ATTACCTAAC CAGAGATTGG 
TCTATCCTGG GACCCCACCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCC 
GGAGCCAAGG GCCGCATCAA GCACTTGGAT GTGGTTGCCC TGCTGAGACG CATCCAGCCC 
CCATTGGGAT TTGGAAAGCT ATGCCCACAC CGAGTGGCCT GCAAGAGACT CGTGGCAATG 
AATGTGCCCC TCAACTCAGA TGGAACAGTG ACATTCAACG CTACACTCTT TGCCCTGGTG 
CGGACATCCC TGAAGATCAA GACAGAAGGG AACCTGGATC AAGCCAACCA GGAGCTTCGG 
ATGGTCATCA AAAAGATCTG GAAGCGGATA AAGCAGAAAT TGTTGGATGA GGTCATCCCT 
CCTCCCGATG AGGAGGAGGT CACTGTGGGA AAATTCTATG CCACATTCCT GATCCAAGAT 
TATTTCCGAA AATTCCGGAG AAGGAAAGAA AAGGGGCTAC TAGGAAGAGA GGCCCCAACA 
AGCACATCCT CTGCCCTCCA GGCTGGTCTA AGGAGCCTGC AGGACTTGGG TCCTGAGATC 
CGTCAAGCCC TCACCTATGA CACTGAGGAA GAAGAGGAAG AGGAAGAGGC AGTGGGTCAG 
GAGGCTGAGG AAGAGGAAGC TGAGAACAAC CCAGAACCAT ACAAAGACTC CATAGACTCC 
CAGCCCCAAT CTCGATGGAA CTCTAGGATT TCGGTGTCTC TACCTGTTAA GGAGAAACTT 
CCAGATTCTC TCTCAACTGG GCCGAGTGAT GATGATGGGC TGGCTCCCAA CTCCAGGCAG 
CCCAGTGTGA TACAGGCTGG CTCCCAACCA CACAGGAGAA GCTCTGGGGT TTTCATGTTC 
ACTATCCCGG AAGAAGGAAG TATTCAGCTC AAGGGAACTC AAGGGCAGGA CAATCAGAAT 
GAGGAACAGG AAGTCCCTGA CTGGACTCCT GACCTGGATG AGCAGGCCGG GACTCCTTCG 
AACCCAGTCC TTTTACCACC TCACTGGTCC CAGCAACACG TAAACGGGCA CCATGTGCCA 
CGCCGACGTT TGCTGCCCCC CACGCCTGCA GGTCGGAAGC CCTCCTTCAC CATCCAGTGT 
CTGCAACGCC AGGGCAGTTG TGAAGATTTA CCTATCCCAG GCACCTACCA TCGTGGACGG 
ACCTCAGGAC CAAGCAGGGC TCAGGGTTCC TGGGCAGCCC CTCCTCAGAA GGGTCGACTG 
CTATATGCCC CCCTGTTGTT GGTGGAGGAA TCTACAGTGG GTGAAGGATA CCTTGGCAAA 
CTTGGCGGCC CACTGCGTAC CTTCACCTGT CTGCAAGTGC CTGGAGCTCA TCCGAATCCC 
AGCCACCGCA AGAGGGGCAG TGCTGACAGT TTGGTGGAGG CTGTGCTCAT CTCCGAAGGC 
CTAGGTCTCT TTGCCCAAGA CCCACGATTT GTGGCCCTGG CCAAGCAGGA GATTGCAGAT 
GCATGTCACC TGACCCTGGA TGAGATGGAC AGTGCTGCCA GTGACCTGCT GGCACAGAGA 
ACCACCTCCC TTTACAGTGA TGAGGAGTCT ATTCTTTCCC GCTTTGATGA AGAGGACCTG 
GGAGATGAGA TGGCCTGTGT CCATGCCCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107268.1
CDS	1..5793
Translation	MSESEVGKGERSHPPLKTLGISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRSCTLNHTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTDTPGDEDEEEGTMASCTRCLNKIMKTRICRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPLWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGREKSSEGNPPKENKVLVPGGENEDAKGARSEGAGMEEEEEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNVRTGLCANPSILSLEPSFTWSSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSSFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLKIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGREAPTSTSSALQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAVGQEAEEEEAENNPEPYKDSIDSQPQSRWNSRISVSLPVKEKLPDSLSTGPSDDDGLAPNSRQPSVIQAGSQPHRRSSGVFMFTIPEEGSIQLKGTQGQDNQNEEQEVPDWTPDLDEQAGTPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPSRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAHPNPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_030251408.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251408.1

ORF Insert Sequence:

ATGTCGGAAT CTGAAGTCGG GAAAGGTGAG AGAAGCCATC CACCCCTGAA GACCCTTGGC 
ATCAGCATTG TAGAGTGGAA GCCTTTTGAT ATTCTCATCC TCCTGACAAT CTTTGCCAAC 
TGCGTGGCAT TGGGGGTATA TATCCCCTTC CCTGAGGACG ACTCCAACAC TGCTAACCAC 
AACTTGGAAC AGGTAGAATA CGTGTTCCTG GTGATTTTCA CCGTGGAGAC AGTGCTCAAG 
ATCGTAGCCT ATGGGCTGGT GCTCCATCCC AGCGCCTATA TTCGCAATGG CTGGAACCTG 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTGC TGCTGGAACA AGGACCTGGG 
CGGCCAGGAG ATGCCCCGCA TACTGGAGGA AAGCCAGGAG GCTTCGATGT AAAGGCACTG 
CGGGCATTTA GGGTGCTACG ACCTCTAAGG CTAGTGTCTG GGGTCCCGAG TCTGCACATA 
GTGCTCAATT CCATCATGAA GGCGCTTGTG CCGCTGCTGC ACATTGCCCT GTTGGTGCTC 
TTCGTCATTA TCATTTACGC CATCATCGGA CTCGAGCTAT TCCTCGGACG AATGCACAAG 
ACATGCTACT TCCTGGGATC TGATATGGAA GCAGAGGAGG ACCCATCACC TTGTGCATCT 
TCTGGCTCTG GGCGTTCATG CACACTGAAC CATACCGAGT GCCGCGGGCG CTGGCCAGGA 
CCCAACGGTG GCATCACGAA CTTCGACAAT TTTTTCTTTG CCATGCTAAC TGTGTTCCAG 
TGTATTACCA TGGAAGGCTG GACAGACGTC CTCTACTGGA TGCAGGATGC CATGGGGTAT 
GAGCTGCCTT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT TGTCCTCAAC 
CTTGTGCTTG GAGTCCTAAG CGGGGAGTTC TCCAAGGAAA GAGAAAAGGC AAAAGCACGA 
GGTGACTTTC AGAAGCTTCG GGAGAAGCAG CAGATGGAAG AAGACCTTCG GGGCTACCTG 
GACTGGATCA CACAGGCTGA GGAGTTAGAC CTTCATGACC CCTCAGTAGA CGGCAACTTG 
GCTTCTCTTG CTGAAGAGGG ACGGGCGGGC CATCGGCCAC AACTGTCAGA GCTGACCAAT 
AGGAGGCGCG GACGGCTGCG ATGGTTCAGC CACTCTACTC GCTCCACACA CTCCACCAGC 
AGCCACGCCA GCCTCCCAGC CAGTGACACT GGCTCCATGA CAGACACCCC TGGAGATGAG 
GATGAAGAAG AGGGGACCAT GGCTAGCTGT ACACGCTGCC TAAACAAGAT TATGAAAACA 
AGGATCTGCC GCCACTTCCG CCGAGCCAAC CGGGGTCTCC GTGCACGCTG CCGCCGGGCC 
GTCAAGTCCA ACGCCTGCTA CTGGGCTGTA CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATAGCTTCAG AGCACCATGG GCAGCCTTTG TGGCTCACCC AGACCCAAGA GTATGCCAAC 
AAAGTTCTGC TCTGCCTCTT CACTGTGGAG ATGCTCCTCA AACTGTACGG CCTGGGCCCC 
TCTGTCTACG TTGCCTCCTT TTTCAACCGC TTTGACTGCT TCGTGGTCTG TGGGGGCATC 
CTAGAAACCA CTTTGGTGGA GGTGGGGGCC ATGCAGCCTC TTGGCATCTC AGTGCTCCGA 
TGTGTACGTC TCCTCAGGAT CTTCAAGGTC ACCAGGCACT GGGCATCCCT GAGCAATCTG 
GTGGCATCTT TGCTCAATTC CATGAAGTCC ATCGCCTCCT TGCTGCTTCT CCTCTTTCTC 
TTCATCATCA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGAGGAG CACCTTTGAT ACCTTCCCCC AAGCCCTCCT CACTGTCTTT 
CAGATCCTGA CTGGTGAGGA TTGGAACGTT GTCATGTATG ATGGTATCAT GGCCTACGGT 
GGGCCCTTCT TCCCAGGGAT GCTGGTGTGT GTTTATTTCA TCATCCTCTT CATCTGTGGC 
AACTACATCC TGCTGAACGT GTTTCTTGCC ATTGCCGTGG ATAACCTAGC CAGCGGGGAT 
GCAGGCACTG CCAAAGACAA GGGCAGAGAG AAGAGCAGTG AAGGAAACCC TCCAAAGGAG 
AACAAAGTAT TGGTGCCTGG TGGAGAGAAT GAGGACGCAA AGGGTGCAAG AAGTGAAGGA 
GCAGGCATGG AGGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGA GGAAGAGGAA 
AATGGTGCAG GACATGTGGA ACTCTTGCAG GAAGTAGTAC CCAAGGAGAA GGTGGTACCC 
ATCCCTGAAG GCAGTGCCTT CTTCTGCCTT AGCCAAACCA ACCCGCTTCG GAAGGCCTGC 
CACACACTCA TACATCACCA TATCTTCACC AGTCTCATCC TAGTGTTCAT CATCCTCAGT 
AGTGTGTCCC TGGCTGCTGA GGACCCCATC CGAGCTCACT CCTTCCGAAA CCATATTCTG 
GGATATTTTG ATTATGCCTT CACCTCCATA TTCACTGTGG AGATTCTACT CAAGATGACA 
GTGTTTGGGG CCTTCCTGCA CCGAGGCTCT TTCTGCCGTA GCTGGTTCAA TCTGTTGGAT 
CTCCTTGTGG TCAGTGTGTC CCTCATCTCC TTCGGCATCC ACTCCAGTGC CATCTCAGTT 
GTGAAGATTC TCCGAGTCCT CCGAGTCCTG CGGCCTCTCC GAGCCATCAA CAGAGCCAAG 
GGACTCAAGC ATGTGGTGCA GTGTGTGTTC GTGGCCATCC GGACCATCGG AAACATCATG 
ATTGTCACCA CCCTCTTGCA GTTCATGTTC GCCTGCATTG GTGTTCAGCT GTTCAAGGGA 
AAATTCTACA GTTGCACTGA TGAGGCCAAA CACACCCTGA AAGAATGCAA GGGCTCCTTC 
CTCATCTACC CTGATGGAGA TGTGTCACGA CCTTTGGTCC GGGAGCGGCT CTGGGTCAAC 
AGTGATTTTA ACTTTGACAA CGTCCTTTCA GCCATGATGG CCCTGTTCAC TGTCTCTACC 
TTTGAAGGCT GGCCTGCGCT ACTATACAAG GCCATAGATG CAAACGCAGA AGATGAGGGC 
CCTATCTACA ATTACCATGT GGAGATATCA GTATTCTTCA TTGTCTACAT CATCATCATC 
GCCTTCTTCA TGATGAACAT CTTTGTGGGC TTTGTTATCA TCACATTCCG TGCCCAGGGA 
GAGCAGGAGT ACCAAAACTG TGAACTGGAC AAGAACCAGC GCCAGTGTGT GGAATATGCC 
CTCAAAGCTC AGCCACTCCG CCGATACATC CCTAAGAATC CTCATCAGTA CCGCGTGTGG 
GCCACTGTGA ACTCTGCTGC CTTTGAGTAC CTCATGTTTC TGCTCATCCT GCTCAACACG 
GTGGCCCTAG CCATGCAGCA CTATGAACAG ACTGCTCCCT TTAACTATGC CATGGACATC 
CTCAACATGG TCTTCACTGG CCTCTTCACC ATTGAGATGG TGCTCAAAAT CATCGCCTTT 
AAACCCAAGC ATTACTTTGC AGATGCCTGG AATACGTTTG ATGCTCTCAT TGTAGTGGGC 
AGTGTAGTCG ACATCGCCGT CACAGAAGTC AATGTGAGGA CTGGGCTTTG TGCTAACCCC 
TCTATCCTCT CCCTAGAGCC TTCTTTCACT TGGAGTTCAG AGGACAGCTC CCGCATATCT 
ATCACGTTCT TTCGCCTCTT CCGAGTCATG AGGCTGGTCA AGCTTCTGAG TAAGGGTGAG 
GGGATCCGCA CACTGCTCTG GACATTCATC AAGTCTTTCC AGGCCTTGCC CTATGTGGCA 
CTTCTCATAG CAATGATATT CTTCATCTAT GCAGTCATTG GCATGCAGAT GTTTGGCAAG 
GTGGCTCTTC AGGACGGCAC GCAGATAAAT CGAAACAACA ATTTCCAGAC CTTTCCGCAG 
GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCCT GGCAAGAGAT AATGCTAGCC 
AGCCTTCCAG GAAATCGATG TGACCCTGAG TCTGACTTTG GCCCAGGCGA GGAATTTACC 
TGTGGTAGCA GTTTTGCCAT CGTCTACTTC ATCAGCTTCT TTATGCTCTG TGCCTTCCTG 
ATTATAAATC TCTTTGTGGC TGTAATCATG GATAACTTTG ATTACCTAAC CAGAGATTGG 
TCTATCCTGG GACCCCACCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCC 
GGAGCCAAGG GCCGCATCAA GCACTTGGAT GTGGTTGCCC TGCTGAGACG CATCCAGCCC 
CCATTGGGAT TTGGAAAGCT ATGCCCACAC CGAGTGGCCT GCAAGAGACT CGTGGCAATG 
AATGTGCCCC TCAACTCAGA TGGAACAGTG ACATTCAACG CTACACTCTT TGCCCTGGTG 
CGGACATCCC TGAAGATCAA GACAGAAGGG AACCTGGATC AAGCCAACCA GGAGCTTCGG 
ATGGTCATCA AAAAGATCTG GAAGCGGATA AAGCAGAAAT TGTTGGATGA GGTCATCCCT 
CCTCCCGATG AGGAGGAGGT CACTGTGGGA AAATTCTATG CCACATTCCT GATCCAAGAT 
TATTTCCGAA AATTCCGGAG AAGGAAAGAA AAGGGGCTAC TAGGAAGAGA GGCCCCAACA 
AGCACATCCT CTGCCCTCCA GGCTGGTCTA AGGAGCCTGC AGGACTTGGG TCCTGAGATC 
CGTCAAGCCC TCACCTATGA CACTGAGGAA GAAGAGGAAG AGGAAGAGGC AGTGGGTCAG 
GAGGCTGAGG AAGAGGAAGC TGAGAACAAC CCAGAACCAT ACAAAGACTC CATAGACTCC 
CAGCCCCAAT CTCGATGGAA CTCTAGGATT TCGGTGTCTC TACCTGTTAA GGAGAAACTT 
CCAGATTCTC TCTCAACTGG GCCGAGTGAT GATGATGGGC TGGCTCCCAA CTCCAGGCAG 
CCCAGTGTGA TACAGGCTGG CTCCCAACCA CACAGGAGAA GCTCTGGGGT TTTCATGTTC 
ACTATCCCGG AAGAAGGAAG TATTCAGCTC AAGGGAACTC AAGGGCAGGA CAATCAGAAT 
GAGGAACAGG AAGTCCCTGA CTGGACTCCT GACCTGGATG AGCAGGCCGG GACTCCTTCG 
AACCCAGTCC TTTTACCACC TCACTGGTCC CAGCAACACG TAAACGGGCA CCATGTGCCA 
CGCCGACGTT TGCTGCCCCC CACGCCTGCA GGTCGGAAGC CCTCCTTCAC CATCCAGTGT 
CTGCAACGCC AGGGCAGTTG TGAAGATTTA CCTATCCCAG GCACCTACCA TCGTGGACGG 
ACCTCAGGAC CAAGCAGGGC TCAGGGTTCC TGGGCAGCCC CTCCTCAGAA GGGTCGACTG 
CTATATGCCC CCCTGTTGTT GGTGGAGGAA TCTACAGTGG GTGAAGGATA CCTTGGCAAA 
CTTGGCGGCC CACTGCGTAC CTTCACCTGT CTGCAAGTGC CTGGAGCTCA TCCGAATCCC 
AGCCACCGCA AGAGGGGCAG TGCTGACAGT TTGGTGGAGG CTGTGCTCAT CTCCGAAGGC 
CTAGGTCTCT TTGCCCAAGA CCCACGATTT GTGGCCCTGG CCAAGCAGGA GATTGCAGAT 
GCATGTCACC TGACCCTGGA TGAGATGGAC AGTGCTGCCA GTGACCTGCT GGCACAGAGA 
ACCACCTCCC TTTACAGTGA TGAGGAGTCT ATTCTTTCCC GCTTTGATGA AGAGGACCTG 
GGAGATGAGA TGGCCTGTGT CCATGCCCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1f ( NM_019582.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062528.2 Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), mRNA.

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CloneID	OMu15776
Clone ID Related Accession (Same CDS sequence)	NM_019582.2
Accession Version	NM_019582.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1F
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672231.7 and AJ579852.2. On Oct 7, 2006 this sequence version replaced NM_019582.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF192497.1, AJ579852.2 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAAT CTGAAGTCGG GAAAGATACA ACCCCAGAGC CCAGTCCAGC CAATGGGACT 
GGCCCTGGCC CTGAATGGGG GCTCTGTCCT GGGCCTCCAA CTGTGGGGAC TGATACCAGC 
GGGGCGTCAG GCCTGGGGAC CCCAAGAAGA AGGACCCAGC ACAACAAACA CAAGACTGTG 
GCGGTGGCCA GTGCTCAGAG ATCACCTCGA GCGCTCTTCT GCCTCACCCT TACTAATCCC 
ATTCGTCGGT CCTGCATCAG CATTGTAGAG TGGAAGCCTT TTGATATTCT CATCCTCCTG 
ACAATCTTTG CCAACTGCGT GGCATTGGGG GTATATATCC CCTTCCCTGA GGACGACTCC 
AACACTGCTA ACCACAACTT GGAACAGGTA GAATACGTGT TCCTGGTGAT TTTCACCGTG 
GAGACAGTGC TCAAGATCGT AGCCTATGGG CTGGTGCTCC ATCCCAGCGC CTATATTCGC 
AATGGCTGGA ACCTGCTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG 
GAACAAGGAC CTGGGCGGCC AGGAGATGCC CCGCATACTG GAGGAAAGCC AGGAGGCTTC 
GATGTAAAGG CACTGCGGGC ATTTAGGGTG CTACGACCTC TAAGGCTAGT GTCTGGGGTC 
CCGAGTCTGC ACATAGTGCT CAATTCCATC ATGAAGGCGC TTGTGCCGCT GCTGCACATT 
GCCCTGTTGG TGCTCTTCGT CATTATCATT TACGCCATCA TCGGACTCGA GCTATTCCTC 
GGACGAATGC ACAAGACATG CTACTTCCTG GGATCTGATA TGGAAGCAGA GGAGGACCCA 
TCACCTTGTG CATCTTCTGG CTCTGGGCGT TCATGCACAC TGAACCATAC CGAGTGCCGC 
GGGCGCTGGC CAGGACCCAA CGGTGGCATC ACGAACTTCG ACAATTTTTT CTTTGCCATG 
CTAACTGTGT TCCAGTGTAT TACCATGGAA GGCTGGACAG ACGTCCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT GCCTTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTTGTCC TCAACCTTGT GCTTGGAGTC CTAAGCGGGG AGTTCTCCAA GGAAAGAGAA 
AAGGCAAAAG CACGAGGTGA CTTTCAGAAG CTTCGGGAGA AGCAGCAGAT GGAAGAAGAC 
CTTCGGGGCT ACCTGGACTG GATCACACAG GCTGAGGAGT TAGACCTTCA TGACCCCTCA 
GTAGACGGCA ACTTGGCTTC TCTTGCTGAA GAGGGACGGG CGGGCCATCG GCCACAACTG 
TCAGAGCTGA CCAATAGGAG GCGCGGACGG CTGCGATGGT TCAGCCACTC TACTCGCTCC 
ACACACTCCA CCAGCAGCCA CGCCAGCCTC CCAGCCAGTG ACACTGGCTC CATGACAGAC 
ACCCCTGGAG ATGAGGATGA AGAAGAGGGG ACCATGGCTA GCTGTACACG CTGCCTAAAC 
AAGATTATGA AAACAAGGAT CTGCCGCCAC TTCCGCCGAG CCAACCGGGG TCTCCGTGCA 
CGCTGCCGCC GGGCCGTCAA GTCCAACGCC TGCTACTGGG CTGTACTGTT GCTCGTCTTC 
CTCAACACGT TGACCATAGC TTCAGAGCAC CATGGGCAGC CTTTGTGGCT CACCCAGACC 
CAAGAGTATG CCAACAAAGT TCTGCTCTGC CTCTTCACTG TGGAGATGCT CCTCAAACTG 
TACGGCCTGG GCCCCTCTGT CTACGTTGCC TCCTTTTTCA ACCGCTTTGA CTGCTTCGTG 
GTCTGTGGGG GCATCCTAGA AACCACTTTG GTGGAGGTGG GGGCCATGCA GCCTCTTGGC 
ATCTCAGTGC TCCGATGTGT ACGTCTCCTC AGGATCTTCA AGGTCACCAG GCACTGGGCA 
TCCCTGAGCA ATCTGGTGGC ATCTTTGCTC AATTCCATGA AGTCCATCGC CTCCTTGCTG 
CTTCTCCTCT TTCTCTTCAT CATCATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACCAAG AGGAGCACCT TTGATACCTT CCCCCAAGCC 
CTCCTCACTG TCTTTCAGAT CCTGACTGGT GAGGATTGGA ACGTTGTCAT GTATGATGGT 
ATCATGGCCT ACGGTGGGCC CTTCTTCCCA GGGATGCTGG TGTGTGTTTA TTTCATCATC 
CTCTTCATCT GTGGCAACTA CATCCTGCTG AACGTGTTTC TTGCCATTGC CGTGGATAAC 
CTAGCCAGCG GGGATGCAGG CACTGCCAAA GACAAGGGCA GAGAGAAGAG CAGTGAAGGA 
AACCCTCCAA AGGAGAACAA AGTATTGGTG CCTGGTGGAG AGAATGAGGA CGCAAAGGGT 
GCAAGAAGTG AAGGAGCAGC ACCAGGCATG GAGGAGGAGG AGGAGGAGGA AGAAGAAGAA 
GAGGAGGAGG AGGAAGAGGA AAATGGTGCA GGACATGTGG AACTCTTGCA GGAAGTAGTA 
CCCAAGGAGA AGGTGGTACC CATCCCTGAA GGCAGTGCCT TCTTCTGCCT TAGCCAAACC 
AACCCGCTTC GGAAGGCCTG CCACACACTC ATACATCACC ATATCTTCAC CAGTCTCATC 
CTAGTGTTCA TCATCCTCAG TAGTGTGTCC CTGGCTGCTG AGGACCCCAT CCGAGCTCAC 
TCCTTCCGAA ACCATATTCT GGGATATTTT GATTATGCCT TCACCTCCAT ATTCACTGTG 
GAGATTCTAC TCAAGATGAC AGTGTTTGGG GCCTTCCTGC ACCGAGGCTC TTTCTGCCGT 
AGCTGGTTCA ATCTGTTGGA TCTCCTTGTG GTCAGTGTGT CCCTCATCTC CTTCGGCATC 
CACTCCAGTG CCATCTCAGT TGTGAAGATT CTCCGAGTCC TCCGAGTCCT GCGGCCTCTC 
CGAGCCATCA ACAGAGCCAA GGGACTCAAG CATGTGGTGC AGTGTGTGTT CGTGGCCATC 
CGGACCATCG GAAACATCAT GATTGTCACC ACCCTCTTGC AGTTCATGTT CGCCTGCATT 
GGTGTTCAGC TGTTCAAGGG AAAATTCTAC AGTTGCACTG ATGAGGCCAA ACACACCCTG 
AAAGAATGCA AGGGCTCCTT CCTCATCTAC CCTGATGGAG ATGTGTCACG ACCTTTGGTC 
CGGGAGCGGC TCTGGGTCAA CAGTGATTTT AACTTTGACA ACGTCCTTTC AGCCATGATG 
GCCCTGTTCA CTGTCTCTAC CTTTGAAGGC TGGCCTGCGC TACTATACAA GGCCATAGAT 
GCAAACGCAG AAGATGAGGG CCCTATCTAC AATTACCATG TGGAGATATC AGTATTCTTC 
ATTGTCTACA TCATCATCAT CGCCTTCTTC ATGATGAACA TCTTTGTGGG CTTTGTTATC 
ATCACATTCC GTGCCCAGGG AGAGCAGGAG TACCAAAACT GTGAACTGGA CAAGAACCAG 
CGCCAGTGTG TGGAATATGC CCTCAAAGCT CAGCCACTCC GCCGATACAT CCCTAAGAAT 
CCTCATCAGT ACCGCGTGTG GGCCACTGTG AACTCTGCTG CCTTTGAGTA CCTCATGTTT 
CTGCTCATCC TGCTCAACAC GGTGGCCCTA GCCATGCAGC ACTATGAACA GACTGCTCCC 
TTTAACTATG CCATGGACAT CCTCAACATG GTCTTCACTG GCCTCTTCAC CATTGAGATG 
GTGCTCAAAA TCATCGCCTT TAAACCCAAG CATTACTTTG CAGATGCCTG GAATACGTTT 
GATGCTCTCA TTGTAGTGGG CAGTGTAGTC GACATCGCCG TCACAGAAGT CAATAACGGA 
GGCCATCTTG GCGAGAGTTC AGAGGACAGC TCCCGCATAT CTATCACGTT CTTTCGCCTC 
TTCCGAGTCA TGAGGCTGGT CAAGCTTCTG AGTAAGGGTG AGGGGATCCG CACACTGCTC 
TGGACATTCA TCAAGTCTTT CCAGGCCTTG CCCTATGTGG CACTTCTCAT AGCAATGATA 
TTCTTCATCT ATGCAGTCAT TGGCATGCAG ATGTTTGGCA AGGTGGCTCT TCAGGACGGC 
ACGCAGATAA ATCGAAACAA CAATTTCCAG ACCTTTCCGC AGGCTGTGCT GCTTCTGTTC 
AGGTGTGCCA CTGGTGAGGC CTGGCAAGAG ATAATGCTAG CCAGCCTTCC AGGAAATCGA 
TGTGACCCTG AGTCTGACTT TGGCCCAGGC GAGGAATTTA CCTGTGGTAG CAGTTTTGCC 
ATCGTCTACT TCATCAGCTT CTTTATGCTC TGTGCCTTCC TGATTATAAA TCTCTTTGTG 
GCTGTAATCA TGGATAACTT TGATTACCTA ACCAGAGATT GGTCTATCCT GGGACCCCAC 
CACCTTGATG AATTCAAGAG GATCTGGTCT GAATATGACC CCGGAGCCAA GGGCCGCATC 
AAGCACTTGG ATGTGGTTGC CCTGCTGAGA CGCATCCAGC CCCCATTGGG ATTTGGAAAG 
CTATGCCCAC ACCGAGTGGC CTGCAAGAGA CTCGTGGCAA TGAATGTGCC CCTCAACTCA 
GATGGAACAG TGACATTCAA CGCTACACTC TTTGCCCTGG TGCGGACATC CCTGAAGATC 
AAGACAGAAG GGAACCTGGA TCAAGCCAAC CAGGAGCTTC GGATGGTCAT CAAAAAGATC 
TGGAAGCGGA TAAAGCAGAA ATTGTTGGAT GAGGTCATCC CTCCTCCCGA TGAGGAGGAG 
GTCACTGTGG GAAAATTCTA TGCCACATTC CTGATCCAAG ATTATTTCCG AAAATTCCGG 
AGAAGGAAAG AAAAGGGGCT ACTAGGAAGA GAGGCCCCAA CAAGCACATC CTCTGCCCTC 
CAGGCTGGTC TAAGGAGCCT GCAGGACTTG GGTCCTGAGA TCCGTCAAGC CCTCACCTAT 
GACACTGAGG AAGAAGAGGA AGAGGAAGAG GCAGTGGGTC AGGAGGCTGA GGAAGAGGAA 
GCTGAGAACA ACCCAGAACC ATACAAAGAC TCCATAGACT CCCAGCCCCA ATCTCGATGG 
AACTCTAGGA TTTCGGTGTC TCTACCTGTT AAGGAGAAAC TTCCAGATTC TCTCTCAACT 
GGGCCGAGTG ATGATGATGG GCTGGCTCCC AACTCCAGGC AGCCCAGTGT GATACAGGCT 
GGCTCCCAAC CACACAGGAG AAGCTCTGGG GTTTTCATGT TCACTATCCC GGAAGAAGGA 
AGTATTCAGC TCAAGGGAAC TCAAGGGCAG GACAATCAGA ATGAGGAACA GGAAGTCCCT 
GACTGGACTC CTGACCTGGA TGAGCAGGCC GGGACTCCTT CGAACCCAGT CCTTTTACCA 
CCTCACTGGT CCCAGCAACA CGTAAACGGG CACCATGTGC CACGCCGACG TTTGCTGCCC 
CCCACGCCTG CAGGTCGGAA GCCCTCCTTC ACCATCCAGT GTCTGCAACG CCAGGGCAGT 
TGTGAAGATT TACCTATCCC AGGCACCTAC CATCGTGGAC GGACCTCAGG ACCAAGCAGG 
GCTCAGGGTT CCTGGGCAGC CCCTCCTCAG AAGGGTCGAC TGCTATATGC CCCCCTGTTG 
TTGGTGGAGG AATCTACAGT GGGTGAAGGA TACCTTGGCA AACTTGGCGG CCCACTGCGT 
ACCTTCACCT GTCTGCAAGT GCCTGGAGCT CATCCGAATC CCAGCCACCG CAAGAGGGGC 
AGTGCTGACA GTTTGGTGGA GGCTGTGCTC ATCTCCGAAG GCCTAGGTCT CTTTGCCCAA 
GACCCACGAT TTGTGGCCCT GGCCAAGCAG GAGATTGCAG ATGCATGTCA CCTGACCCTG 
GATGAGATGG ACAGTGCTGC CAGTGACCTG CTGGCACAGA GAACCACCTC CCTTTACAGT 
GATGAGGAGT CTATTCTTTC CCGCTTTGAT GAAGAGGACC TGGGAGATGA GATGGCCTGT 
GTCCATGCCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062528.2
CDS	44..5998
Translation	MSESEVGKDTTPEPSPANGTGPGPEWGLCPGPPTVGTDTSGASGLGTPRRRTQHNKHKTVAVASAQRSPRALFCLTLTNPIRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRSCTLNHTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTDTPGDEDEEEGTMASCTRCLNKIMKTRICRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPLWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGREKSSEGNPPKENKVLVPGGENEDAKGARSEGAAPGMEEEEEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSSFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLKIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGREAPTSTSSALQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAVGQEAEEEEAENNPEPYKDSIDSQPQSRWNSRISVSLPVKEKLPDSLSTGPSDDDGLAPNSRQPSVIQAGSQPHRRSSGVFMFTIPEEGSIQLKGTQGQDNQNEEQEVPDWTPDLDEQAGTPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPSRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAHPNPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_019582.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019582.2

ORF Insert Sequence:

ATGTCGGAAT CTGAAGTCGG GAAAGATACA ACCCCAGAGC CCAGTCCAGC CAATGGGACT 
GGCCCTGGCC CTGAATGGGG GCTCTGTCCT GGGCCTCCAA CTGTGGGGAC TGATACCAGC 
GGGGCGTCAG GCCTGGGGAC CCCAAGAAGA AGGACCCAGC ACAACAAACA CAAGACTGTG 
GCGGTGGCCA GTGCTCAGAG ATCACCTCGA GCGCTCTTCT GCCTCACCCT TACTAATCCC 
ATTCGTCGGT CCTGCATCAG CATTGTAGAG TGGAAGCCTT TTGATATTCT CATCCTCCTG 
ACAATCTTTG CCAACTGCGT GGCATTGGGG GTATATATCC CCTTCCCTGA GGACGACTCC 
AACACTGCTA ACCACAACTT GGAACAGGTA GAATACGTGT TCCTGGTGAT TTTCACCGTG 
GAGACAGTGC TCAAGATCGT AGCCTATGGG CTGGTGCTCC ATCCCAGCGC CTATATTCGC 
AATGGCTGGA ACCTGCTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG 
GAACAAGGAC CTGGGCGGCC AGGAGATGCC CCGCATACTG GAGGAAAGCC AGGAGGCTTC 
GATGTAAAGG CACTGCGGGC ATTTAGGGTG CTACGACCTC TAAGGCTAGT GTCTGGGGTC 
CCGAGTCTGC ACATAGTGCT CAATTCCATC ATGAAGGCGC TTGTGCCGCT GCTGCACATT 
GCCCTGTTGG TGCTCTTCGT CATTATCATT TACGCCATCA TCGGACTCGA GCTATTCCTC 
GGACGAATGC ACAAGACATG CTACTTCCTG GGATCTGATA TGGAAGCAGA GGAGGACCCA 
TCACCTTGTG CATCTTCTGG CTCTGGGCGT TCATGCACAC TGAACCATAC CGAGTGCCGC 
GGGCGCTGGC CAGGACCCAA CGGTGGCATC ACGAACTTCG ACAATTTTTT CTTTGCCATG 
CTAACTGTGT TCCAGTGTAT TACCATGGAA GGCTGGACAG ACGTCCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT GCCTTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTTGTCC TCAACCTTGT GCTTGGAGTC CTAAGCGGGG AGTTCTCCAA GGAAAGAGAA 
AAGGCAAAAG CACGAGGTGA CTTTCAGAAG CTTCGGGAGA AGCAGCAGAT GGAAGAAGAC 
CTTCGGGGCT ACCTGGACTG GATCACACAG GCTGAGGAGT TAGACCTTCA TGACCCCTCA 
GTAGACGGCA ACTTGGCTTC TCTTGCTGAA GAGGGACGGG CGGGCCATCG GCCACAACTG 
TCAGAGCTGA CCAATAGGAG GCGCGGACGG CTGCGATGGT TCAGCCACTC TACTCGCTCC 
ACACACTCCA CCAGCAGCCA CGCCAGCCTC CCAGCCAGTG ACACTGGCTC CATGACAGAC 
ACCCCTGGAG ATGAGGATGA AGAAGAGGGG ACCATGGCTA GCTGTACACG CTGCCTAAAC 
AAGATTATGA AAACAAGGAT CTGCCGCCAC TTCCGCCGAG CCAACCGGGG TCTCCGTGCA 
CGCTGCCGCC GGGCCGTCAA GTCCAACGCC TGCTACTGGG CTGTACTGTT GCTCGTCTTC 
CTCAACACGT TGACCATAGC TTCAGAGCAC CATGGGCAGC CTTTGTGGCT CACCCAGACC 
CAAGAGTATG CCAACAAAGT TCTGCTCTGC CTCTTCACTG TGGAGATGCT CCTCAAACTG 
TACGGCCTGG GCCCCTCTGT CTACGTTGCC TCCTTTTTCA ACCGCTTTGA CTGCTTCGTG 
GTCTGTGGGG GCATCCTAGA AACCACTTTG GTGGAGGTGG GGGCCATGCA GCCTCTTGGC 
ATCTCAGTGC TCCGATGTGT ACGTCTCCTC AGGATCTTCA AGGTCACCAG GCACTGGGCA 
TCCCTGAGCA ATCTGGTGGC ATCTTTGCTC AATTCCATGA AGTCCATCGC CTCCTTGCTG 
CTTCTCCTCT TTCTCTTCAT CATCATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACCAAG AGGAGCACCT TTGATACCTT CCCCCAAGCC 
CTCCTCACTG TCTTTCAGAT CCTGACTGGT GAGGATTGGA ACGTTGTCAT GTATGATGGT 
ATCATGGCCT ACGGTGGGCC CTTCTTCCCA GGGATGCTGG TGTGTGTTTA TTTCATCATC 
CTCTTCATCT GTGGCAACTA CATCCTGCTG AACGTGTTTC TTGCCATTGC CGTGGATAAC 
CTAGCCAGCG GGGATGCAGG CACTGCCAAA GACAAGGGCA GAGAGAAGAG CAGTGAAGGA 
AACCCTCCAA AGGAGAACAA AGTATTGGTG CCTGGTGGAG AGAATGAGGA CGCAAAGGGT 
GCAAGAAGTG AAGGAGCAGC ACCAGGCATG GAGGAGGAGG AGGAGGAGGA AGAAGAAGAA 
GAGGAGGAGG AGGAAGAGGA AAATGGTGCA GGACATGTGG AACTCTTGCA GGAAGTAGTA 
CCCAAGGAGA AGGTGGTACC CATCCCTGAA GGCAGTGCCT TCTTCTGCCT TAGCCAAACC 
AACCCGCTTC GGAAGGCCTG CCACACACTC ATACATCACC ATATCTTCAC CAGTCTCATC 
CTAGTGTTCA TCATCCTCAG TAGTGTGTCC CTGGCTGCTG AGGACCCCAT CCGAGCTCAC 
TCCTTCCGAA ACCATATTCT GGGATATTTT GATTATGCCT TCACCTCCAT ATTCACTGTG 
GAGATTCTAC TCAAGATGAC AGTGTTTGGG GCCTTCCTGC ACCGAGGCTC TTTCTGCCGT 
AGCTGGTTCA ATCTGTTGGA TCTCCTTGTG GTCAGTGTGT CCCTCATCTC CTTCGGCATC 
CACTCCAGTG CCATCTCAGT TGTGAAGATT CTCCGAGTCC TCCGAGTCCT GCGGCCTCTC 
CGAGCCATCA ACAGAGCCAA GGGACTCAAG CATGTGGTGC AGTGTGTGTT CGTGGCCATC 
CGGACCATCG GAAACATCAT GATTGTCACC ACCCTCTTGC AGTTCATGTT CGCCTGCATT 
GGTGTTCAGC TGTTCAAGGG AAAATTCTAC AGTTGCACTG ATGAGGCCAA ACACACCCTG 
AAAGAATGCA AGGGCTCCTT CCTCATCTAC CCTGATGGAG ATGTGTCACG ACCTTTGGTC 
CGGGAGCGGC TCTGGGTCAA CAGTGATTTT AACTTTGACA ACGTCCTTTC AGCCATGATG 
GCCCTGTTCA CTGTCTCTAC CTTTGAAGGC TGGCCTGCGC TACTATACAA GGCCATAGAT 
GCAAACGCAG AAGATGAGGG CCCTATCTAC AATTACCATG TGGAGATATC AGTATTCTTC 
ATTGTCTACA TCATCATCAT CGCCTTCTTC ATGATGAACA TCTTTGTGGG CTTTGTTATC 
ATCACATTCC GTGCCCAGGG AGAGCAGGAG TACCAAAACT GTGAACTGGA CAAGAACCAG 
CGCCAGTGTG TGGAATATGC CCTCAAAGCT CAGCCACTCC GCCGATACAT CCCTAAGAAT 
CCTCATCAGT ACCGCGTGTG GGCCACTGTG AACTCTGCTG CCTTTGAGTA CCTCATGTTT 
CTGCTCATCC TGCTCAACAC GGTGGCCCTA GCCATGCAGC ACTATGAACA GACTGCTCCC 
TTTAACTATG CCATGGACAT CCTCAACATG GTCTTCACTG GCCTCTTCAC CATTGAGATG 
GTGCTCAAAA TCATCGCCTT TAAACCCAAG CATTACTTTG CAGATGCCTG GAATACGTTT 
GATGCTCTCA TTGTAGTGGG CAGTGTAGTC GACATCGCCG TCACAGAAGT CAATAACGGA 
GGCCATCTTG GCGAGAGTTC AGAGGACAGC TCCCGCATAT CTATCACGTT CTTTCGCCTC 
TTCCGAGTCA TGAGGCTGGT CAAGCTTCTG AGTAAGGGTG AGGGGATCCG CACACTGCTC 
TGGACATTCA TCAAGTCTTT CCAGGCCTTG CCCTATGTGG CACTTCTCAT AGCAATGATA 
TTCTTCATCT ATGCAGTCAT TGGCATGCAG ATGTTTGGCA AGGTGGCTCT TCAGGACGGC 
ACGCAGATAA ATCGAAACAA CAATTTCCAG ACCTTTCCGC AGGCTGTGCT GCTTCTGTTC 
AGGTGTGCCA CTGGTGAGGC CTGGCAAGAG ATAATGCTAG CCAGCCTTCC AGGAAATCGA 
TGTGACCCTG AGTCTGACTT TGGCCCAGGC GAGGAATTTA CCTGTGGTAG CAGTTTTGCC 
ATCGTCTACT TCATCAGCTT CTTTATGCTC TGTGCCTTCC TGATTATAAA TCTCTTTGTG 
GCTGTAATCA TGGATAACTT TGATTACCTA ACCAGAGATT GGTCTATCCT GGGACCCCAC 
CACCTTGATG AATTCAAGAG GATCTGGTCT GAATATGACC CCGGAGCCAA GGGCCGCATC 
AAGCACTTGG ATGTGGTTGC CCTGCTGAGA CGCATCCAGC CCCCATTGGG ATTTGGAAAG 
CTATGCCCAC ACCGAGTGGC CTGCAAGAGA CTCGTGGCAA TGAATGTGCC CCTCAACTCA 
GATGGAACAG TGACATTCAA CGCTACACTC TTTGCCCTGG TGCGGACATC CCTGAAGATC 
AAGACAGAAG GGAACCTGGA TCAAGCCAAC CAGGAGCTTC GGATGGTCAT CAAAAAGATC 
TGGAAGCGGA TAAAGCAGAA ATTGTTGGAT GAGGTCATCC CTCCTCCCGA TGAGGAGGAG 
GTCACTGTGG GAAAATTCTA TGCCACATTC CTGATCCAAG ATTATTTCCG AAAATTCCGG 
AGAAGGAAAG AAAAGGGGCT ACTAGGAAGA GAGGCCCCAA CAAGCACATC CTCTGCCCTC 
CAGGCTGGTC TAAGGAGCCT GCAGGACTTG GGTCCTGAGA TCCGTCAAGC CCTCACCTAT 
GACACTGAGG AAGAAGAGGA AGAGGAAGAG GCAGTGGGTC AGGAGGCTGA GGAAGAGGAA 
GCTGAGAACA ACCCAGAACC ATACAAAGAC TCCATAGACT CCCAGCCCCA ATCTCGATGG 
AACTCTAGGA TTTCGGTGTC TCTACCTGTT AAGGAGAAAC TTCCAGATTC TCTCTCAACT 
GGGCCGAGTG ATGATGATGG GCTGGCTCCC AACTCCAGGC AGCCCAGTGT GATACAGGCT 
GGCTCCCAAC CACACAGGAG AAGCTCTGGG GTTTTCATGT TCACTATCCC GGAAGAAGGA 
AGTATTCAGC TCAAGGGAAC TCAAGGGCAG GACAATCAGA ATGAGGAACA GGAAGTCCCT 
GACTGGACTC CTGACCTGGA TGAGCAGGCC GGGACTCCTT CGAACCCAGT CCTTTTACCA 
CCTCACTGGT CCCAGCAACA CGTAAACGGG CACCATGTGC CACGCCGACG TTTGCTGCCC 
CCCACGCCTG CAGGTCGGAA GCCCTCCTTC ACCATCCAGT GTCTGCAACG CCAGGGCAGT 
TGTGAAGATT TACCTATCCC AGGCACCTAC CATCGTGGAC GGACCTCAGG ACCAAGCAGG 
GCTCAGGGTT CCTGGGCAGC CCCTCCTCAG AAGGGTCGAC TGCTATATGC CCCCCTGTTG 
TTGGTGGAGG AATCTACAGT GGGTGAAGGA TACCTTGGCA AACTTGGCGG CCCACTGCGT 
ACCTTCACCT GTCTGCAAGT GCCTGGAGCT CATCCGAATC CCAGCCACCG CAAGAGGGGC 
AGTGCTGACA GTTTGGTGGA GGCTGTGCTC ATCTCCGAAG GCCTAGGTCT CTTTGCCCAA 
GACCCACGAT TTGTGGCCCT GGCCAAGCAG GAGATTGCAG ATGCATGTCA CCTGACCCTG 
GATGAGATGG ACAGTGCTGC CAGTGACCTG CTGGCACAGA GAACCACCTC CCTTTACAGT 
GATGAGGAGT CTATTCTTTC CCGCTTTGAT GAAGAGGACC TGGGAGATGA GATGGCCTGT 
GTCCATGCCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1f ( XM_011247482.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245784.1 Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X2, mRNA.

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CloneID	OMu69566
Clone ID Related Accession (Same CDS sequence)	XM_011247482.2
Accession Version	XM_011247482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAT CTGAAGTCGG GAAAGGTGAG AGAAGCCATC CACCCCTGAA GACCCTTGGC 
ATCAGCATTG TAGAGTGGAA GCCTTTTGAT ATTCTCATCC TCCTGACAAT CTTTGCCAAC 
TGCGTGGCAT TGGGGGTATA TATCCCCTTC CCTGAGGACG ACTCCAACAC TGCTAACCAC 
AACTTGGAAC AGGTAGAATA CGTGTTCCTG GTGATTTTCA CCGTGGAGAC AGTGCTCAAG 
ATCGTAGCCT ATGGGCTGGT GCTCCATCCC AGCGCCTATA TTCGCAATGG CTGGAACCTG 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTGC TGCTGGAACA AGGACCTGGG 
CGGCCAGGAG ATGCCCCGCA TACTGGAGGA AAGCCAGGAG GCTTCGATGT AAAGGCACTG 
CGGGCATTTA GGGTGCTACG ACCTCTAAGG CTAGTGTCTG GGGTCCCGAG TCTGCACATA 
GTGCTCAATT CCATCATGAA GGCGCTTGTG CCGCTGCTGC ACATTGCCCT GTTGGTGCTC 
TTCGTCATTA TCATTTACGC CATCATCGGA CTCGAGCTAT TCCTCGGACG AATGCACAAG 
ACATGCTACT TCCTGGGATC TGATATGGAA GCAGAGGAGG ACCCATCACC TTGTGCATCT 
TCTGGCTCTG GGCGTTCATG CACACTGAAC CATACCGAGT GCCGCGGGCG CTGGCCAGGA 
CCCAACGGTG GCATCACGAA CTTCGACAAT TTTTTCTTTG CCATGCTAAC TGTGTTCCAG 
TGTATTACCA TGGAAGGCTG GACAGACGTC CTCTACTGGA TGCAGGATGC CATGGGGTAT 
GAGCTGCCTT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT TGTCCTCAAC 
CTTGTGCTTG GAGTCCTAAG CGGGGAGTTC TCCAAGGAAA GAGAAAAGGC AAAAGCACGA 
GGTGACTTTC AGAAGCTTCG GGAGAAGCAG CAGATGGAAG AAGACCTTCG GGGCTACCTG 
GACTGGATCA CACAGGCTGA GGAGTTAGAC CTTCATGACC CCTCAGTAGA CGGCAACTTG 
GCTTCTCTTG CTGAAGAGGG ACGGGCGGGC CATCGGCCAC AACTGTCAGA GCTGACCAAT 
AGGAGGCGCG GACGGCTGCG ATGGTTCAGC CACTCTACTC GCTCCACACA CTCCACCAGC 
AGCCACGCCA GCCTCCCAGC CAGTGACACT GGCTCCATGA CAGACACCCC TGGAGATGAG 
GATGAAGAAG AGGGGACCAT GGCTAGCTGT ACACGCTGCC TAAACAAGAT TATGAAAACA 
AGGATCTGCC GCCACTTCCG CCGAGCCAAC CGGGGTCTCC GTGCACGCTG CCGCCGGGCC 
GTCAAGTCCA ACGCCTGCTA CTGGGCTGTA CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATAGCTTCAG AGCACCATGG GCAGCCTTTG TGGCTCACCC AGACCCAAGA GTATGCCAAC 
AAAGTTCTGC TCTGCCTCTT CACTGTGGAG ATGCTCCTCA AACTGTACGG CCTGGGCCCC 
TCTGTCTACG TTGCCTCCTT TTTCAACCGC TTTGACTGCT TCGTGGTCTG TGGGGGCATC 
CTAGAAACCA CTTTGGTGGA GGTGGGGGCC ATGCAGCCTC TTGGCATCTC AGTGCTCCGA 
TGTGTACGTC TCCTCAGGAT CTTCAAGGTC ACCAGGCACT GGGCATCCCT GAGCAATCTG 
GTGGCATCTT TGCTCAATTC CATGAAGTCC ATCGCCTCCT TGCTGCTTCT CCTCTTTCTC 
TTCATCATCA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGAGGAG CACCTTTGAT ACCTTCCCCC AAGCCCTCCT CACTGTCTTT 
CAGATCCTGA CTGGTGAGGA TTGGAACGTT GTCATGTATG ATGGTATCAT GGCCTACGGT 
GGGCCCTTCT TCCCAGGGAT GCTGGTGTGT GTTTATTTCA TCATCCTCTT CATCTGTGGC 
AACTACATCC TGCTGAACGT GTTTCTTGCC ATTGCCGTGG ATAACCTAGC CAGCGGGGAT 
GCAGGCACTG CCAAAGACAA GGGCAGAGAG AAGAGCAGTG AAGGAAACCC TCCAAAGGAG 
AACAAAGTAT TGGTGCCTGG TGGAGAGAAT GAGGACGCAA AGGGTGCAAG AAGTGAAGGA 
GCAGGCATGG AGGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGA GGAAGAGGAA 
AATGGTGCAG GACATGTGGA ACTCTTGCAG GAAGTAGTAC CCAAGGAGAA GGTGGTACCC 
ATCCCTGAAG GCAGTGCCTT CTTCTGCCTT AGCCAAACCA ACCCGCTTCG GAAGGCCTGC 
CACACACTCA TACATCACCA TATCTTCACC AGTCTCATCC TAGTGTTCAT CATCCTCAGT 
AGTGTGTCCC TGGCTGCTGA GGACCCCATC CGAGCTCACT CCTTCCGAAA CCATATTCTG 
GGATATTTTG ATTATGCCTT CACCTCCATA TTCACTGTGG AGATTCTACT CAAGATGACA 
GTGTTTGGGG CCTTCCTGCA CCGAGGCTCT TTCTGCCGTA GCTGGTTCAA TCTGTTGGAT 
CTCCTTGTGG TCAGTGTGTC CCTCATCTCC TTCGGCATCC ACTCCAGTGC CATCTCAGTT 
GTGAAGATTC TCCGAGTCCT CCGAGTCCTG CGGCCTCTCC GAGCCATCAA CAGAGCCAAG 
GGACTCAAGC ATGTGGTGCA GTGTGTGTTC GTGGCCATCC GGACCATCGG AAACATCATG 
ATTGTCACCA CCCTCTTGCA GTTCATGTTC GCCTGCATTG GTGTTCAGCT GTTCAAGGGA 
AAATTCTACA GTTGCACTGA TGAGGCCAAA CACACCCTGA AAGAATGCAA GGGCTCCTTC 
CTCATCTACC CTGATGGAGA TGTGTCACGA CCTTTGGTCC GGGAGCGGCT CTGGGTCAAC 
AGTGATTTTA ACTTTGACAA CGTCCTTTCA GCCATGATGG CCCTGTTCAC TGTCTCTACC 
TTTGAAGGCT GGCCTGCGCT ACTATACAAG GCCATAGATG CAAACGCAGA AGATGAGGGC 
CCTATCTACA ATTACCATGT GGAGATATCA GTATTCTTCA TTGTCTACAT CATCATCATC 
GCCTTCTTCA TGATGAACAT CTTTGTGGGC TTTGTTATCA TCACATTCCG TGCCCAGGGA 
GAGCAGGAGT ACCAAAACTG TGAACTGGAC AAGAACCAGC GCCAGTGTGT GGAATATGCC 
CTCAAAGCTC AGCCACTCCG CCGATACATC CCTAAGAATC CTCATCAGTA CCGCGTGTGG 
GCCACTGTGA ACTCTGCTGC CTTTGAGTAC CTCATGTTTC TGCTCATCCT GCTCAACACG 
GTGGCCCTAG CCATGCAGCA CTATGAACAG ACTGCTCCCT TTAACTATGC CATGGACATC 
CTCAACATGG TCTTCACTGG CCTCTTCACC ATTGAGATGG TGCTCAAAAT CATCGCCTTT 
AAACCCAAGC ATTACTTTGC AGATGCCTGG AATACGTTTG ATGCTCTCAT TGTAGTGGGC 
AGTGTAGTCG ACATCGCCGT CACAGAAGTC AATAACGGAG GCCATCTTGG CGAGAGTTCA 
GAGGACAGCT CCCGCATATC TATCACGTTC TTTCGCCTCT TCCGAGTCAT GAGGCTGGTC 
AAGCTTCTGA GTAAGGGTGA GGGGATCCGC ACACTGCTCT GGACATTCAT CAAGTCTTTC 
CAGGCCTTGC CCTATGTGGC ACTTCTCATA GCAATGATAT TCTTCATCTA TGCAGTCATT 
GGCATGCAGA TGTTTGGCAA GGTGGCTCTT CAGGACGGCA CGCAGATAAA TCGAAACAAC 
AATTTCCAGA CCTTTCCGCA GGCTGTGCTG CTTCTGTTCA GGTGTGCCAC TGGTGAGGCC 
TGGCAAGAGA TAATGCTAGC CAGCCTTCCA GGAAATCGAT GTGACCCTGA GTCTGACTTT 
GGCCCAGGCG AGGAATTTAC CTGTGGTAGC AGTTTTGCCA TCGTCTACTT CATCAGCTTC 
TTTATGCTCT GTGCCTTCCT GATTATAAAT CTCTTTGTGG CTGTAATCAT GGATAACTTT 
GATTACCTAA CCAGAGATTG GTCTATCCTG GGACCCCACC ACCTTGATGA ATTCAAGAGG 
ATCTGGTCTG AATATGACCC CGGAGCCAAG GGCCGCATCA AGCACTTGGA TGTGGTTGCC 
CTGCTGAGAC GCATCCAGCC CCCATTGGGA TTTGGAAAGC TATGCCCACA CCGAGTGGCC 
TGCAAGAGAC TCGTGGCAAT GAATGTGCCC CTCAACTCAG ATGGAACAGT GACATTCAAC 
GCTACACTCT TTGCCCTGGT GCGGACATCC CTGAAGATCA AGACAGAAGG GAACCTGGAT 
CAAGCCAACC AGGAGCTTCG GATGGTCATC AAAAAGATCT GGAAGCGGAT AAAGCAGAAA 
TTGTTGGATG AGGTCATCCC TCCTCCCGAT GAGGAGGAGG TCACTGTGGG AAAATTCTAT 
GCCACATTCC TGATCCAAGA TTATTTCCGA AAATTCCGGA GAAGGAAAGA AAAGGGGCTA 
CTAGGAAGAG AGGCCCCAAC AAGCACATCC TCTGCCCTCC AGGCTGGTCT AAGGAGCCTG 
CAGGACTTGG GTCCTGAGAT CCGTCAAGCC CTCACCTATG ACACTGAGGA AGAAGAGGAA 
GAGGAAGAGG CAGTGGGTCA GGAGGCTGAG GAAGAGGAAG CTGAGAACAA CCCAGAACCA 
TACAAAGACT CCATAGACTC CCAGCCCCAA TCTCGATGGA ACTCTAGGAT TTCGGTGTCT 
CTACCTGTTA AGGAGAAACT TCCAGATTCT CTCTCAACTG GGCCGAGTGA TGATGATGGG 
CTGGCTCCCA ACTCCAGGCA GCCCAGTGTG ATACAGGCTG GCTCCCAACC ACACAGGAGA 
AGCTCTGGGG TTTTCATGTT CACTATCCCG GAAGAAGGAA GTATTCAGCT CAAGGGAACT 
CAAGGGCAGG ACAATCAGAA TGAGGAACAG GAAGTCCCTG ACTGGACTCC TGACCTGGAT 
GAGCAGGCCG GGACTCCTTC GAACCCAGTC CTTTTACCAC CTCACTGGTC CCAGCAACAC 
GTAAACGGGC ACCATGTGCC ACGCCGACGT TTGCTGCCCC CCACGCCTGC AGGTCGGAAG 
CCCTCCTTCA CCATCCAGTG TCTGCAACGC CAGGGCAGTT GTGAAGATTT ACCTATCCCA 
GGCACCTACC ATCGTGGACG GACCTCAGGA CCAAGCAGGG CTCAGGGTTC CTGGGCAGCC 
CCTCCTCAGA AGGGTCGACT GCTATATGCC CCCCTGTTGT TGGTGGAGGA ATCTACAGTG 
GGTGAAGGAT ACCTTGGCAA ACTTGGCGGC CCACTGCGTA CCTTCACCTG TCTGCAAGTG 
CCTGGAGCTC ATCCGAATCC CAGCCACCGC AAGAGGGGCA GTGCTGACAG TTTGGTGGAG 
GCTGTGCTCA TCTCCGAAGG CCTAGGTCTC TTTGCCCAAG ACCCACGATT TGTGGCCCTG 
GCCAAGCAGG AGATTGCAGA TGCATGTCAC CTGACCCTGG ATGAGATGGA CAGTGCTGCC 
AGTGACCTGC TGGCACAGAG AACCACCTCC CTTTACAGTG ATGAGGAGTC TATTCTTTCC 
CGCTTTGATG AAGAGGACCT GGGAGATGAG ATGGCCTGTG TCCATGCCCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245784.1
CDS	77..5830
Translation	MSESEVGKGERSHPPLKTLGISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRSCTLNHTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTDTPGDEDEEEGTMASCTRCLNKIMKTRICRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPLWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGREKSSEGNPPKENKVLVPGGENEDAKGARSEGAGMEEEEEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSSFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLKIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGREAPTSTSSALQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAVGQEAEEEEAENNPEPYKDSIDSQPQSRWNSRISVSLPVKEKLPDSLSTGPSDDDGLAPNSRQPSVIQAGSQPHRRSSGVFMFTIPEEGSIQLKGTQGQDNQNEEQEVPDWTPDLDEQAGTPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPSRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAHPNPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_011247482.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1F subunit (Cacna1f), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247482.2

ORF Insert Sequence:

ATGTCGGAAT CTGAAGTCGG GAAAGGTGAG AGAAGCCATC CACCCCTGAA GACCCTTGGC 
ATCAGCATTG TAGAGTGGAA GCCTTTTGAT ATTCTCATCC TCCTGACAAT CTTTGCCAAC 
TGCGTGGCAT TGGGGGTATA TATCCCCTTC CCTGAGGACG ACTCCAACAC TGCTAACCAC 
AACTTGGAAC AGGTAGAATA CGTGTTCCTG GTGATTTTCA CCGTGGAGAC AGTGCTCAAG 
ATCGTAGCCT ATGGGCTGGT GCTCCATCCC AGCGCCTATA TTCGCAATGG CTGGAACCTG 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTGC TGCTGGAACA AGGACCTGGG 
CGGCCAGGAG ATGCCCCGCA TACTGGAGGA AAGCCAGGAG GCTTCGATGT AAAGGCACTG 
CGGGCATTTA GGGTGCTACG ACCTCTAAGG CTAGTGTCTG GGGTCCCGAG TCTGCACATA 
GTGCTCAATT CCATCATGAA GGCGCTTGTG CCGCTGCTGC ACATTGCCCT GTTGGTGCTC 
TTCGTCATTA TCATTTACGC CATCATCGGA CTCGAGCTAT TCCTCGGACG AATGCACAAG 
ACATGCTACT TCCTGGGATC TGATATGGAA GCAGAGGAGG ACCCATCACC TTGTGCATCT 
TCTGGCTCTG GGCGTTCATG CACACTGAAC CATACCGAGT GCCGCGGGCG CTGGCCAGGA 
CCCAACGGTG GCATCACGAA CTTCGACAAT TTTTTCTTTG CCATGCTAAC TGTGTTCCAG 
TGTATTACCA TGGAAGGCTG GACAGACGTC CTCTACTGGA TGCAGGATGC CATGGGGTAT 
GAGCTGCCTT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT TGTCCTCAAC 
CTTGTGCTTG GAGTCCTAAG CGGGGAGTTC TCCAAGGAAA GAGAAAAGGC AAAAGCACGA 
GGTGACTTTC AGAAGCTTCG GGAGAAGCAG CAGATGGAAG AAGACCTTCG GGGCTACCTG 
GACTGGATCA CACAGGCTGA GGAGTTAGAC CTTCATGACC CCTCAGTAGA CGGCAACTTG 
GCTTCTCTTG CTGAAGAGGG ACGGGCGGGC CATCGGCCAC AACTGTCAGA GCTGACCAAT 
AGGAGGCGCG GACGGCTGCG ATGGTTCAGC CACTCTACTC GCTCCACACA CTCCACCAGC 
AGCCACGCCA GCCTCCCAGC CAGTGACACT GGCTCCATGA CAGACACCCC TGGAGATGAG 
GATGAAGAAG AGGGGACCAT GGCTAGCTGT ACACGCTGCC TAAACAAGAT TATGAAAACA 
AGGATCTGCC GCCACTTCCG CCGAGCCAAC CGGGGTCTCC GTGCACGCTG CCGCCGGGCC 
GTCAAGTCCA ACGCCTGCTA CTGGGCTGTA CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATAGCTTCAG AGCACCATGG GCAGCCTTTG TGGCTCACCC AGACCCAAGA GTATGCCAAC 
AAAGTTCTGC TCTGCCTCTT CACTGTGGAG ATGCTCCTCA AACTGTACGG CCTGGGCCCC 
TCTGTCTACG TTGCCTCCTT TTTCAACCGC TTTGACTGCT TCGTGGTCTG TGGGGGCATC 
CTAGAAACCA CTTTGGTGGA GGTGGGGGCC ATGCAGCCTC TTGGCATCTC AGTGCTCCGA 
TGTGTACGTC TCCTCAGGAT CTTCAAGGTC ACCAGGCACT GGGCATCCCT GAGCAATCTG 
GTGGCATCTT TGCTCAATTC CATGAAGTCC ATCGCCTCCT TGCTGCTTCT CCTCTTTCTC 
TTCATCATCA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGAGGAG CACCTTTGAT ACCTTCCCCC AAGCCCTCCT CACTGTCTTT 
CAGATCCTGA CTGGTGAGGA TTGGAACGTT GTCATGTATG ATGGTATCAT GGCCTACGGT 
GGGCCCTTCT TCCCAGGGAT GCTGGTGTGT GTTTATTTCA TCATCCTCTT CATCTGTGGC 
AACTACATCC TGCTGAACGT GTTTCTTGCC ATTGCCGTGG ATAACCTAGC CAGCGGGGAT 
GCAGGCACTG CCAAAGACAA GGGCAGAGAG AAGAGCAGTG AAGGAAACCC TCCAAAGGAG 
AACAAAGTAT TGGTGCCTGG TGGAGAGAAT GAGGACGCAA AGGGTGCAAG AAGTGAAGGA 
GCAGGCATGG AGGAGGAGGA GGAGGAGGAA GAAGAAGAAG AGGAGGAGGA GGAAGAGGAA 
AATGGTGCAG GACATGTGGA ACTCTTGCAG GAAGTAGTAC CCAAGGAGAA GGTGGTACCC 
ATCCCTGAAG GCAGTGCCTT CTTCTGCCTT AGCCAAACCA ACCCGCTTCG GAAGGCCTGC 
CACACACTCA TACATCACCA TATCTTCACC AGTCTCATCC TAGTGTTCAT CATCCTCAGT 
AGTGTGTCCC TGGCTGCTGA GGACCCCATC CGAGCTCACT CCTTCCGAAA CCATATTCTG 
GGATATTTTG ATTATGCCTT CACCTCCATA TTCACTGTGG AGATTCTACT CAAGATGACA 
GTGTTTGGGG CCTTCCTGCA CCGAGGCTCT TTCTGCCGTA GCTGGTTCAA TCTGTTGGAT 
CTCCTTGTGG TCAGTGTGTC CCTCATCTCC TTCGGCATCC ACTCCAGTGC CATCTCAGTT 
GTGAAGATTC TCCGAGTCCT CCGAGTCCTG CGGCCTCTCC GAGCCATCAA CAGAGCCAAG 
GGACTCAAGC ATGTGGTGCA GTGTGTGTTC GTGGCCATCC GGACCATCGG AAACATCATG 
ATTGTCACCA CCCTCTTGCA GTTCATGTTC GCCTGCATTG GTGTTCAGCT GTTCAAGGGA 
AAATTCTACA GTTGCACTGA TGAGGCCAAA CACACCCTGA AAGAATGCAA GGGCTCCTTC 
CTCATCTACC CTGATGGAGA TGTGTCACGA CCTTTGGTCC GGGAGCGGCT CTGGGTCAAC 
AGTGATTTTA ACTTTGACAA CGTCCTTTCA GCCATGATGG CCCTGTTCAC TGTCTCTACC 
TTTGAAGGCT GGCCTGCGCT ACTATACAAG GCCATAGATG CAAACGCAGA AGATGAGGGC 
CCTATCTACA ATTACCATGT GGAGATATCA GTATTCTTCA TTGTCTACAT CATCATCATC 
GCCTTCTTCA TGATGAACAT CTTTGTGGGC TTTGTTATCA TCACATTCCG TGCCCAGGGA 
GAGCAGGAGT ACCAAAACTG TGAACTGGAC AAGAACCAGC GCCAGTGTGT GGAATATGCC 
CTCAAAGCTC AGCCACTCCG CCGATACATC CCTAAGAATC CTCATCAGTA CCGCGTGTGG 
GCCACTGTGA ACTCTGCTGC CTTTGAGTAC CTCATGTTTC TGCTCATCCT GCTCAACACG 
GTGGCCCTAG CCATGCAGCA CTATGAACAG ACTGCTCCCT TTAACTATGC CATGGACATC 
CTCAACATGG TCTTCACTGG CCTCTTCACC ATTGAGATGG TGCTCAAAAT CATCGCCTTT 
AAACCCAAGC ATTACTTTGC AGATGCCTGG AATACGTTTG ATGCTCTCAT TGTAGTGGGC 
AGTGTAGTCG ACATCGCCGT CACAGAAGTC AATAACGGAG GCCATCTTGG CGAGAGTTCA 
GAGGACAGCT CCCGCATATC TATCACGTTC TTTCGCCTCT TCCGAGTCAT GAGGCTGGTC 
AAGCTTCTGA GTAAGGGTGA GGGGATCCGC ACACTGCTCT GGACATTCAT CAAGTCTTTC 
CAGGCCTTGC CCTATGTGGC ACTTCTCATA GCAATGATAT TCTTCATCTA TGCAGTCATT 
GGCATGCAGA TGTTTGGCAA GGTGGCTCTT CAGGACGGCA CGCAGATAAA TCGAAACAAC 
AATTTCCAGA CCTTTCCGCA GGCTGTGCTG CTTCTGTTCA GGTGTGCCAC TGGTGAGGCC 
TGGCAAGAGA TAATGCTAGC CAGCCTTCCA GGAAATCGAT GTGACCCTGA GTCTGACTTT 
GGCCCAGGCG AGGAATTTAC CTGTGGTAGC AGTTTTGCCA TCGTCTACTT CATCAGCTTC 
TTTATGCTCT GTGCCTTCCT GATTATAAAT CTCTTTGTGG CTGTAATCAT GGATAACTTT 
GATTACCTAA CCAGAGATTG GTCTATCCTG GGACCCCACC ACCTTGATGA ATTCAAGAGG 
ATCTGGTCTG AATATGACCC CGGAGCCAAG GGCCGCATCA AGCACTTGGA TGTGGTTGCC 
CTGCTGAGAC GCATCCAGCC CCCATTGGGA TTTGGAAAGC TATGCCCACA CCGAGTGGCC 
TGCAAGAGAC TCGTGGCAAT GAATGTGCCC CTCAACTCAG ATGGAACAGT GACATTCAAC 
GCTACACTCT TTGCCCTGGT GCGGACATCC CTGAAGATCA AGACAGAAGG GAACCTGGAT 
CAAGCCAACC AGGAGCTTCG GATGGTCATC AAAAAGATCT GGAAGCGGAT AAAGCAGAAA 
TTGTTGGATG AGGTCATCCC TCCTCCCGAT GAGGAGGAGG TCACTGTGGG AAAATTCTAT 
GCCACATTCC TGATCCAAGA TTATTTCCGA AAATTCCGGA GAAGGAAAGA AAAGGGGCTA 
CTAGGAAGAG AGGCCCCAAC AAGCACATCC TCTGCCCTCC AGGCTGGTCT AAGGAGCCTG 
CAGGACTTGG GTCCTGAGAT CCGTCAAGCC CTCACCTATG ACACTGAGGA AGAAGAGGAA 
GAGGAAGAGG CAGTGGGTCA GGAGGCTGAG GAAGAGGAAG CTGAGAACAA CCCAGAACCA 
TACAAAGACT CCATAGACTC CCAGCCCCAA TCTCGATGGA ACTCTAGGAT TTCGGTGTCT 
CTACCTGTTA AGGAGAAACT TCCAGATTCT CTCTCAACTG GGCCGAGTGA TGATGATGGG 
CTGGCTCCCA ACTCCAGGCA GCCCAGTGTG ATACAGGCTG GCTCCCAACC ACACAGGAGA 
AGCTCTGGGG TTTTCATGTT CACTATCCCG GAAGAAGGAA GTATTCAGCT CAAGGGAACT 
CAAGGGCAGG ACAATCAGAA TGAGGAACAG GAAGTCCCTG ACTGGACTCC TGACCTGGAT 
GAGCAGGCCG GGACTCCTTC GAACCCAGTC CTTTTACCAC CTCACTGGTC CCAGCAACAC 
GTAAACGGGC ACCATGTGCC ACGCCGACGT TTGCTGCCCC CCACGCCTGC AGGTCGGAAG 
CCCTCCTTCA CCATCCAGTG TCTGCAACGC CAGGGCAGTT GTGAAGATTT ACCTATCCCA 
GGCACCTACC ATCGTGGACG GACCTCAGGA CCAAGCAGGG CTCAGGGTTC CTGGGCAGCC 
CCTCCTCAGA AGGGTCGACT GCTATATGCC CCCCTGTTGT TGGTGGAGGA ATCTACAGTG 
GGTGAAGGAT ACCTTGGCAA ACTTGGCGGC CCACTGCGTA CCTTCACCTG TCTGCAAGTG 
CCTGGAGCTC ATCCGAATCC CAGCCACCGC AAGAGGGGCA GTGCTGACAG TTTGGTGGAG 
GCTGTGCTCA TCTCCGAAGG CCTAGGTCTC TTTGCCCAAG ACCCACGATT TGTGGCCCTG 
GCCAAGCAGG AGATTGCAGA TGCATGTCAC CTGACCCTGG ATGAGATGGA CAGTGCTGCC 
AGTGACCTGC TGGCACAGAG AACCACCTCC CTTTACAGTG ATGAGGAGTC TATTCTTTCC 
CGCTTTGATG AAGAGGACCT GGGAGATGAG ATGGCCTGTG TCCATGCCCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cone dystrophy and ectopic synaptogenesis in a Cacna1f loss of function model of congenital stationary night blindness (CSNB2A).
Channels (Austin, Tex.)12(1)17-33(2018 Jan)
Waldner DM,Giraldo Sierra NC,Bonfield S,Nguyen L,Dimopoulos IS,Sauv? Y,Stell WK,Bech-Hansen NT

A missense mutation in
Journal of neurophysiology118(2)845-854(2017 Aug)
Peachey NS,Hasan N,FitzMaurice B,Burrill S,Pangeni G,Karst SY,Reinholdt L,Berry ML,Strobel M,Gregg RG,McCall MA,Chang B

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Cacna1f cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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