CACNA1F cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1F
Entrez Gene ID	778
Full Name	calcium voltage-gated channel subunit alpha1 F
Synonyms	AIED,COD3,COD4,CORDX,CORDX3,CSNB2,CSNB2A,CSNBX2,Cav1.4,Cav1.4alpha1,JM8,JMC8,OA2
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 F Names voltage-dependent L-type calcium channel subunit alpha-1F calcium channel, voltage-dependent, L type, alpha 1F subunit voltage-gated calcium channel subunit alpha Cav1.4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.23
Summary	This gene encodes a multipass transmembrane protein that functions as an alpha-1 subunit of the voltage-dependent calcium channel, which mediates the influx of calcium ions into the cell. The encoded protein forms a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Mutations in this gene can cause X-linked eye disorders, including congenital stationary night blindness type 2A, cone-rod dystropy, and Aland Island eye disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2013].
Disorder MIM:	300110
Disorder Html:	Night blindness, congenital stationary (incomplete), 2A, X-linked, 300071 (3); Cone-rod dystrophy, X-linked, 3, 300476 (3); Aland Island eye disease, 300600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256790.2	NP_001243719.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform 3
NM_001256789.3	NP_001243718.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform 2
XM_017029836.1	XP_016885325.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
NM_005183.4	NP_005174.2	voltage-dependent L-type calcium channel subunit alpha-1F isoform 1
XM_011543983.2	XP_011542285.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
NM_001256789.2	NP_001243718.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform 2
NM_005183.3	NP_005174.2	voltage-dependent L-type calcium channel subunit alpha-1F isoform 1
NM_001256790.3	NP_001243719.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1F	NP_005174.2
	CACNA1F	XP_001106163.2
Canis lupus familiaris (dog)	CACNA1F	XP_005642146.1
Danio rerio (zebrafish)	LOC559964	XP_003199134.2
Bos taurus (cattle)	CACNA1F	NP_001192592.1
Mus musculus (house mouse)	Cacna1f	NP_062528.2
Danio rerio (zebrafish)	LOC793708	XP_001333514.5
Xenopus tropicalis (tropical clawed frog)	cacna1f	XP_002941018.2
Rattus norvegicus (Norway rat)	Cacna1f	NP_446153.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary insights into T-type Ca
Smith CL, Abdallah S, Wong YY, Le P, Harracksingh AN, Artinian L, Tamvacakis AN, Rehder V, Reese TS, Senatore A
The Journal of general physiology149(4)483-510(2017 Apr)

The importance of genetic testing as demonstrated by two cases of
Men CJ, Bujakowska KM, Comander J, Place E, Bedoukian EC, Zhu X, Leroy BP, Fulton AB, Pierce EA
Molecular vision23695-706(2017)

Alternative splicing at C terminus of Ca(V)1.4 calcium channel modulates calcium-dependent inactivation, activation potential, and current density.
Tan GM, Yu D, Wang J, Soong TW
The Journal of biological chemistry287(2)832-47(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AED, iCSNB, and X-linked cone-rod dystrophy 3 are designations that refer to a broad, continuous spectrum of clinical appearances caused in the majority by a variety of mutations in CACNA1F.
Title: Clinical Characteristics, Mutation Spectrum, and Prevalence of ?land Eye Disease/Incomplete Congenital Stationary Night Blindness in Denmark.

exon 47 encodes structural determinants that regulate CDI and voltage-dependent activation of Cav1.4, and is necessary for modulation of channel activation by CaBP4.
Title: Characterization of C-terminal Splice Variants of Cav1.4 Ca2+ Channels in Human Retina.

a single nucleotide change c.1555C>T in exon 13 of the CACNA1F gene, leading to the substitution of arginine by tryptophan (p.R519W) in a Chinese individual affected by retinitis pigmentosa, is identified.
Title: Establishment and rapid detection of a heterozygous missense mutation in the CACNA1F gene by ARMS technique with double-base mismatched primers.

novel heterozygous missense mutation (c.1555C>T, p.R519W) in CACNA1F gene, which is probably associated with XLRP.
Title: Identification of a novel heterozygous missense mutation in the CACNA1F gene in a chinese family with retinitis pigmentosa by next generation sequencing.

Studies indicate a role for L-type calcium channel Cav1.3 and Cav1.4 in cochlear inner hair cells (IHCs) and retinal photoreceptors (PRs).
Title: Voltage-Gated Cav1 Channels in Disorders of Vision and Hearing.

The following CACNA1F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21305	NM_005183.4 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu99472	XM_017029836.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu21327	NM_001256790.3 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu21801	NM_001256789.3 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu56583	XM_011543983.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu21801	NM_001256789.2	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu21305	NM_005183.3	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu21327	NM_001256790.2	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1F ( NM_005183.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005174.2 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21305
Clone ID Related Accession (Same CDS sequence)	NM_005183.4 , NM_005183.3
Accession Version	NM_005183.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF201304.1 and AC232271.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_005183.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF201304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT 
GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG 
GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT 
CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG 
ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG 
GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC 
GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT 
GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA 
TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC 
GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG 
CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA 
GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG 
AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC 
CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC 
GCCGATGACA ACCTTGGTTC TATGGCTGAA GAGGGCCGGG CGGGCCATCG GCCACAGCTG 
GCCGAGCTGA CCAATAGGAG GCGTGGACGT CTGCGCTGGT TCAGTCATTC TACTCGCTCC 
ACACACTCCA CCAGCAGCCA TGCCAGCCTC CCAGCCAGTG ACACCGGTTC CATGACAGAG 
ACCCAAGGCG ATGAGGATGA GGAGGAGGGG GCTCTGGCCA GCTGTACACG CTGCCTAAAC 
AAGATCATGA AAACCAGAGT CTGCCGCCGC CTCCGCCGAG CCAACCGGGT CCTTCGGGCA 
CGCTGCCGTC GGGCAGTGAA GTCCAATGCC TGCTACTGGG CTGTGCTGTT GCTCGTCTTC 
CTCAACACGT TGACCATCGC CTCTGAGCAC CACGGGCAGC CTGTGTGGCT CACCCAGATC 
CAGGAGTATG CCAACAAAGT GTTGCTCTGT CTGTTCACGG TGGAGATGCT TCTCAAATTG 
TACGGTCTGG GCCCCTCTGC CTATGTGTCT TCCTTCTTCA ACCGCTTTGA CTGCTTTGTG 
GTCTGTGGGG GCATCCTAGA GACCACCTTG GTGGAGGTGG GTGCCATGCA GCCCTTGGGC 
ATCTCAGTGC TCCGATGTGT GCGCCTCCTC AGGATCTTTA AGGTCACCAG ACACTGGGCT 
TCTCTGAGCA ATCTGGTGGC ATCCCTGCTC AATTCAATGA AATCCATCGC ATCCTTGCTG 
CTTCTCCTCT TCCTCTTCAT CATTATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACCAAG CGAAGCACCT TTGACACGTT CCCCCAGGCC 
CTCCTCACTG TCTTTCAGAT CCTGACAGGT GAGGACTGGA ACGTGGTCAT GTATGATGGT 
ATCATGGCAT ATGGTGGCCC CTTCTTCCCA GGAATGTTGG TGTGCATCTA TTTCATCATT 
CTCTTCATCT GTGGCAACTA CATCCTGTTG AACGTGTTTC TTGCCATTGC TGTGGACAAC 
CTGGCCAGTG GAGATGCAGG CACTGCCAAG GACAAGGGCG GGGAGAAGAG CAATGAGAAG 
GATCTCCCAC AGGAGAATGA AGGCCTGGTG CCTGGTGTGG AGAAAGAGGA AGAGGAGGGT 
GCAAGGAGGG AAGGAGCAGA CATGGAGGAG GAGGAGGAGG AGGAAGAAGA GGAAGAAGAG 
GAAGAAGAGG AAGAGGGTGC AGGGGGTGTG GAACTCCTGC AGGAAGTTGT ACCCAAGGAG 
AAGGTGGTAC CCATCCCTGA GGGCAGCGCC TTCTTCTGCC TCAGCCAAAC CAACCCGCTG 
AGGAAGGGCT GCCACACCCT CATCCACCAT CATGTCTTCA CCAATCTTAT CCTGGTGTTC 
ATCATCCTCA GCAGTGTGTC CCTGGCCGCT GAGGACCCCA TCCGAGCCCA CTCCTTCCGC 
AACCATATTC TGGGTTACTT CGATTATGCC TTCACCTCCA TTTTCACTGT GGAGATTCTA 
CTAAAGATGA CAGTGTTTGG GGCCTTCCTG CACCGCGGCT CCTTCTGCCG TAGCTGGTTT 
AATATGTTGG ATCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTTGGCAT CCACTCCAGC 
GCCATCTCGG TGGTGAAGAT TCTGCGAGTA CTCCGAGTAC TGCGGCCCCT CCGAGCCATC 
AACAGGGCCA AGGGACTCAA GCATGTGGTG CAGTGTGTAT TTGTGGCCAT CCGGACCATC 
GGAAACATCA TGATTGTCAC CACACTTCTG CAATTTATGT TCGCCTGCAT CGGGGTGCAG 
CTCTTCAAGG GGAAATTCTA CACCTGCACG GACGAGGCCA AACACACCCC TCAAGAATGC 
AAGGGCTCCT TCCTGGTATA CCCAGATGGA GACGTGTCAC GGCCCCTGGT CCGGGAGCGG 
CTCTGGGTCA ACAGTGATTT CAACTTTGAC AATGTCCTTT CAGCCATGAT GGCCCTGTTC 
ACTGTCTCCA CCTTTGAAGG CTGGCCTGCA CTGCTATACA AGGCCATCGA TGCATATGCA 
GAGGACCACG GCCCCATCTA TAATTACCGT GTGGAGATCT CAGTGTTCTT CATTGTCTAC 
ATCATCATCA TTGCGTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CATCACTTTC 
CGTGCCCAGG GCGAGCAGGA GTACCAAAAC TGTGAGCTGG ACAAGAACCA GCGTCAATGT 
GTGGAATATG CCCTCAAGGC CCAGCCACTC CGCCGTTACA TCCCCAAGAA CCCGCATCAG 
TATCGTGTGT GGGCCACTGT GAACTCTGCT GCCTTTGAGT ACCTGATGTT CCTGCTCATC 
CTGCTCAACA CAGTTGCCCT AGCCATGCAG CACTATGAGC AGACTGCTCC CTTCAACTAT 
GCCATGGACA TCCTCAACAT GGTCTTCACT GGCCTCTTCA CTATTGAGAT GGTGCTCAAA 
ATCATCGCCT TCAAGCCCAA GCATTACTTC ACTGATGCCT GGAACACGTT TGACGCTCTT 
ATTGTGGTGG GCAGCATAGT GGATATTGCC GTCACTGAAG TCAATAATGG TGGCCACCTT 
GGCGAGAGCT CTGAGGACAG CTCCCGCATT TCCATTACCT TCTTTCGCCT CTTCCGAGTT 
ATGCGGCTGG TCAAGCTTCT CAGTAAGGGT GAAGGGATCC GCACATTGCT CTGGACATTC 
ATCAAGTCCT TCCAGGCCTT GCCCTATGTG GCTCTTCTCA TCGCAATGAT ATTCTTCATC 
TATGCCGTCA TTGGCATGCA GATGTTCGGC AAGGTGGCTC TTCAGGATGG CACACAGATA 
AACCGAAACA ACAACTTCCA GACCTTTCCA CAGGCTGTGC TGCTTCTGTT CAGGTGTGCC 
ACTGGTGAGG CATGGCAGGA GATAATGCTT GCCAGCCTTC CCGGAAATCG GTGTGATCCT 
GAGTCTGACT TCGGCCCTGG TGAAGAGTTT ACCTGTGGTA GCAATTTTGC CATCGCCTAT 
TTCATCAGCT TCTTCATGCT CTGTGCCTTC CTGATCATAA ATCTCTTTGT GGCTGTGATC 
ATGGACAACT TTGATTATCT CACCAGAGAT TGGTCCATCC TGGGCCCCCA TCACCTTGAT 
GAATTCAAGA GGATCTGGTC TGAATATGAC CCTGGGGCCA AGGGCCGCAT CAAACACTTG 
GATGTGGTTG CCCTGCTGAG ACGTATCCAG CCCCCTCTGG GATTTGGGAA GCTGTGCCCA 
CACCGAGTGG CCTGCAAGAG ACTTGTGGCA ATGAACATGC CCCTCAACTC AGATGGGACG 
GTGACATTCA ACGCCACACT CTTTGCCCTG GTCCGGACAT CCCTGAAGAT CAAAACAGAA 
GGGAACCTGG AGCAAGCCAA CCAGGAGCTG CGGATTGTCA TCAAAAAGAT CTGGAAGCGG 
ATGAAACAGA AGCTGCTAGA TGAGGTCATC CCCCCACCAG ACGAGGAGGA GGTCACCGTG 
GGCAAATTCT ACGCCACATT TCTGATCCAG GACTATTTCC GCAAATTCCG GCGGAGGAAA 
GAAAAAGGGC TACTAGGCAA CGACGCCGCC CCTAGCACCT CTTCCGCCCT TCAGGCTGGT 
CTGCGGAGCC TGCAGGACTT GGGTCCTGAG ATGCGGCAGG CCCTCACCTG TGACACAGAG 
GAGGAGGAAG AAGAGGGGCA GGAGGGAGTG GAGGAGGAAG ATGAAAAGGA CTTGGAAACT 
AACAAAGCCA CGATGGTCTC CCAGCCCTCA GCTCGCCGGG GCTCCGGGAT TTCTGTGTCT 
CTGCCTGTCG GGGACAGACT TCCAGATTCA CTCTCCTTTG GGCCCAGTGA TGATGACAGG 
GGGACTCCCA CCTCCAGTCA GCCCAGTGTG CCCCAGGCTG GATCCAACAC CCACAGGAGA 
GGCTCTGGGG CTCTCATTTT CACCATCCCA GAAGAAGGAA ATTCTCAGCC CAAGGGAACC 
AAAGGGCAAA ACAAGCAAGA TGAGGATGAG GAAGTCCCTG ATCGGCTTTC CTACCTAGAT 
GAGCAGGCAG GGACTCCCCC GTGCTCAGTC CTTTTGCCAC CTCACAGAGC TCAGAGATAC 
ATGGATGGGC ACCTGGTACC ACGCCGCCGT CTGCTGCCCC CCACACCTGC AGGTCGGAAG 
CCCTCCTTCA CCATCCAGTG TCTGCAGCGC CAGGGCAGTT GTGAGGATTT ACCCATCCCA 
GGCACCTATC ATCGTGGGCG AAATTCAGGG CCCAATAGGG CTCAGGGTTC CTGGGCAACA 
CCACCTCAGC GGGGTCGGCT CCTGTATGCC CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG 
GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC CCACTGCGCA CCTTCACCTG TCTGCACGTG 
CCTGGAACCC ACTCGGACCC CAGCCATGGG AAGAGGGGCA GTGCCGACAG CTTGGTGGAG 
GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC TTTGCTCGAG ACCCACGTTT CGTGGCCCTG 
GCCAAGCAGG AGATTGCAGA TGCGTGTCGC CTGACGCTGG ATGAGATGGA CAATGCTGCC 
AGTGACCTGC TGGCACAGGG AACCAGCTCT CTCTATAGCG ACGAGGAGTC CATCCTCTCC 
CGCTTCGATG AGGAGGACTT GGGAGACGAG ATGGCCTGCG TCCACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005174.2
CDS	32..5965
Translation	MSESEGGKDTTPEPSPANGAGPGPEWGLCPGPPAVEGESSGASGLGTPKRRNQHSKHKTVAVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNLGSMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_005183.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005183.4

ORF Insert Sequence:

ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT 
GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG 
GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT 
CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG 
ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG 
GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC 
GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT 
GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA 
TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC 
GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG 
CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA 
GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG 
AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC 
CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC 
GCCGATGACA ACCTTGGTTC TATGGCTGAA GAGGGCCGGG CGGGCCATCG GCCACAGCTG 
GCCGAGCTGA CCAATAGGAG GCGTGGACGT CTGCGCTGGT TCAGTCATTC TACTCGCTCC 
ACACACTCCA CCAGCAGCCA TGCCAGCCTC CCAGCCAGTG ACACCGGTTC CATGACAGAG 
ACCCAAGGCG ATGAGGATGA GGAGGAGGGG GCTCTGGCCA GCTGTACACG CTGCCTAAAC 
AAGATCATGA AAACCAGAGT CTGCCGCCGC CTCCGCCGAG CCAACCGGGT CCTTCGGGCA 
CGCTGCCGTC GGGCAGTGAA GTCCAATGCC TGCTACTGGG CTGTGCTGTT GCTCGTCTTC 
CTCAACACGT TGACCATCGC CTCTGAGCAC CACGGGCAGC CTGTGTGGCT CACCCAGATC 
CAGGAGTATG CCAACAAAGT GTTGCTCTGT CTGTTCACGG TGGAGATGCT TCTCAAATTG 
TACGGTCTGG GCCCCTCTGC CTATGTGTCT TCCTTCTTCA ACCGCTTTGA CTGCTTTGTG 
GTCTGTGGGG GCATCCTAGA GACCACCTTG GTGGAGGTGG GTGCCATGCA GCCCTTGGGC 
ATCTCAGTGC TCCGATGTGT GCGCCTCCTC AGGATCTTTA AGGTCACCAG ACACTGGGCT 
TCTCTGAGCA ATCTGGTGGC ATCCCTGCTC AATTCAATGA AATCCATCGC ATCCTTGCTG 
CTTCTCCTCT TCCTCTTCAT CATTATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACCAAG CGAAGCACCT TTGACACGTT CCCCCAGGCC 
CTCCTCACTG TCTTTCAGAT CCTGACAGGT GAGGACTGGA ACGTGGTCAT GTATGATGGT 
ATCATGGCAT ATGGTGGCCC CTTCTTCCCA GGAATGTTGG TGTGCATCTA TTTCATCATT 
CTCTTCATCT GTGGCAACTA CATCCTGTTG AACGTGTTTC TTGCCATTGC TGTGGACAAC 
CTGGCCAGTG GAGATGCAGG CACTGCCAAG GACAAGGGCG GGGAGAAGAG CAATGAGAAG 
GATCTCCCAC AGGAGAATGA AGGCCTGGTG CCTGGTGTGG AGAAAGAGGA AGAGGAGGGT 
GCAAGGAGGG AAGGAGCAGA CATGGAGGAG GAGGAGGAGG AGGAAGAAGA GGAAGAAGAG 
GAAGAAGAGG AAGAGGGTGC AGGGGGTGTG GAACTCCTGC AGGAAGTTGT ACCCAAGGAG 
AAGGTGGTAC CCATCCCTGA GGGCAGCGCC TTCTTCTGCC TCAGCCAAAC CAACCCGCTG 
AGGAAGGGCT GCCACACCCT CATCCACCAT CATGTCTTCA CCAATCTTAT CCTGGTGTTC 
ATCATCCTCA GCAGTGTGTC CCTGGCCGCT GAGGACCCCA TCCGAGCCCA CTCCTTCCGC 
AACCATATTC TGGGTTACTT CGATTATGCC TTCACCTCCA TTTTCACTGT GGAGATTCTA 
CTAAAGATGA CAGTGTTTGG GGCCTTCCTG CACCGCGGCT CCTTCTGCCG TAGCTGGTTT 
AATATGTTGG ATCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTTGGCAT CCACTCCAGC 
GCCATCTCGG TGGTGAAGAT TCTGCGAGTA CTCCGAGTAC TGCGGCCCCT CCGAGCCATC 
AACAGGGCCA AGGGACTCAA GCATGTGGTG CAGTGTGTAT TTGTGGCCAT CCGGACCATC 
GGAAACATCA TGATTGTCAC CACACTTCTG CAATTTATGT TCGCCTGCAT CGGGGTGCAG 
CTCTTCAAGG GGAAATTCTA CACCTGCACG GACGAGGCCA AACACACCCC TCAAGAATGC 
AAGGGCTCCT TCCTGGTATA CCCAGATGGA GACGTGTCAC GGCCCCTGGT CCGGGAGCGG 
CTCTGGGTCA ACAGTGATTT CAACTTTGAC AATGTCCTTT CAGCCATGAT GGCCCTGTTC 
ACTGTCTCCA CCTTTGAAGG CTGGCCTGCA CTGCTATACA AGGCCATCGA TGCATATGCA 
GAGGACCACG GCCCCATCTA TAATTACCGT GTGGAGATCT CAGTGTTCTT CATTGTCTAC 
ATCATCATCA TTGCGTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CATCACTTTC 
CGTGCCCAGG GCGAGCAGGA GTACCAAAAC TGTGAGCTGG ACAAGAACCA GCGTCAATGT 
GTGGAATATG CCCTCAAGGC CCAGCCACTC CGCCGTTACA TCCCCAAGAA CCCGCATCAG 
TATCGTGTGT GGGCCACTGT GAACTCTGCT GCCTTTGAGT ACCTGATGTT CCTGCTCATC 
CTGCTCAACA CAGTTGCCCT AGCCATGCAG CACTATGAGC AGACTGCTCC CTTCAACTAT 
GCCATGGACA TCCTCAACAT GGTCTTCACT GGCCTCTTCA CTATTGAGAT GGTGCTCAAA 
ATCATCGCCT TCAAGCCCAA GCATTACTTC ACTGATGCCT GGAACACGTT TGACGCTCTT 
ATTGTGGTGG GCAGCATAGT GGATATTGCC GTCACTGAAG TCAATAATGG TGGCCACCTT 
GGCGAGAGCT CTGAGGACAG CTCCCGCATT TCCATTACCT TCTTTCGCCT CTTCCGAGTT 
ATGCGGCTGG TCAAGCTTCT CAGTAAGGGT GAAGGGATCC GCACATTGCT CTGGACATTC 
ATCAAGTCCT TCCAGGCCTT GCCCTATGTG GCTCTTCTCA TCGCAATGAT ATTCTTCATC 
TATGCCGTCA TTGGCATGCA GATGTTCGGC AAGGTGGCTC TTCAGGATGG CACACAGATA 
AACCGAAACA ACAACTTCCA GACCTTTCCA CAGGCTGTGC TGCTTCTGTT CAGGTGTGCC 
ACTGGTGAGG CATGGCAGGA GATAATGCTT GCCAGCCTTC CCGGAAATCG GTGTGATCCT 
GAGTCTGACT TCGGCCCTGG TGAAGAGTTT ACCTGTGGTA GCAATTTTGC CATCGCCTAT 
TTCATCAGCT TCTTCATGCT CTGTGCCTTC CTGATCATAA ATCTCTTTGT GGCTGTGATC 
ATGGACAACT TTGATTATCT CACCAGAGAT TGGTCCATCC TGGGCCCCCA TCACCTTGAT 
GAATTCAAGA GGATCTGGTC TGAATATGAC CCTGGGGCCA AGGGCCGCAT CAAACACTTG 
GATGTGGTTG CCCTGCTGAG ACGTATCCAG CCCCCTCTGG GATTTGGGAA GCTGTGCCCA 
CACCGAGTGG CCTGCAAGAG ACTTGTGGCA ATGAACATGC CCCTCAACTC AGATGGGACG 
GTGACATTCA ACGCCACACT CTTTGCCCTG GTCCGGACAT CCCTGAAGAT CAAAACAGAA 
GGGAACCTGG AGCAAGCCAA CCAGGAGCTG CGGATTGTCA TCAAAAAGAT CTGGAAGCGG 
ATGAAACAGA AGCTGCTAGA TGAGGTCATC CCCCCACCAG ACGAGGAGGA GGTCACCGTG 
GGCAAATTCT ACGCCACATT TCTGATCCAG GACTATTTCC GCAAATTCCG GCGGAGGAAA 
GAAAAAGGGC TACTAGGCAA CGACGCCGCC CCTAGCACCT CTTCCGCCCT TCAGGCTGGT 
CTGCGGAGCC TGCAGGACTT GGGTCCTGAG ATGCGGCAGG CCCTCACCTG TGACACAGAG 
GAGGAGGAAG AAGAGGGGCA GGAGGGAGTG GAGGAGGAAG ATGAAAAGGA CTTGGAAACT 
AACAAAGCCA CGATGGTCTC CCAGCCCTCA GCTCGCCGGG GCTCCGGGAT TTCTGTGTCT 
CTGCCTGTCG GGGACAGACT TCCAGATTCA CTCTCCTTTG GGCCCAGTGA TGATGACAGG 
GGGACTCCCA CCTCCAGTCA GCCCAGTGTG CCCCAGGCTG GATCCAACAC CCACAGGAGA 
GGCTCTGGGG CTCTCATTTT CACCATCCCA GAAGAAGGAA ATTCTCAGCC CAAGGGAACC 
AAAGGGCAAA ACAAGCAAGA TGAGGATGAG GAAGTCCCTG ATCGGCTTTC CTACCTAGAT 
GAGCAGGCAG GGACTCCCCC GTGCTCAGTC CTTTTGCCAC CTCACAGAGC TCAGAGATAC 
ATGGATGGGC ACCTGGTACC ACGCCGCCGT CTGCTGCCCC CCACACCTGC AGGTCGGAAG 
CCCTCCTTCA CCATCCAGTG TCTGCAGCGC CAGGGCAGTT GTGAGGATTT ACCCATCCCA 
GGCACCTATC ATCGTGGGCG AAATTCAGGG CCCAATAGGG CTCAGGGTTC CTGGGCAACA 
CCACCTCAGC GGGGTCGGCT CCTGTATGCC CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG 
GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC CCACTGCGCA CCTTCACCTG TCTGCACGTG 
CCTGGAACCC ACTCGGACCC CAGCCATGGG AAGAGGGGCA GTGCCGACAG CTTGGTGGAG 
GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC TTTGCTCGAG ACCCACGTTT CGTGGCCCTG 
GCCAAGCAGG AGATTGCAGA TGCGTGTCGC CTGACGCTGG ATGAGATGGA CAATGCTGCC 
AGTGACCTGC TGGCACAGGG AACCAGCTCT CTCTATAGCG ACGAGGAGTC CATCCTCTCC 
CGCTTCGATG AGGAGGACTT GGGAGACGAG ATGGCCTGCG TCCACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( NM_005183.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005174.2 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA.

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CloneID	OHu21305
Clone ID Related Accession (Same CDS sequence)	NM_005183.4 , NM_005183.3
Accession Version	NM_005183.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF201304.1 and AC232271.2. This sequence is a reference standard in the RefSeqGene project. On Aug 21, 2013 this sequence version replaced NM_005183.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF201304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT 
GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG 
GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT 
CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG 
ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG 
GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC 
GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT 
GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA 
TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC 
GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG 
CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA 
GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG 
AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC 
CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC 
GCCGATGACA ACCTTGGTTC TATGGCTGAA GAGGGCCGGG CGGGCCATCG GCCACAGCTG 
GCCGAGCTGA CCAATAGGAG GCGTGGACGT CTGCGCTGGT TCAGTCATTC TACTCGCTCC 
ACACACTCCA CCAGCAGCCA TGCCAGCCTC CCAGCCAGTG ACACCGGTTC CATGACAGAG 
ACCCAAGGCG ATGAGGATGA GGAGGAGGGG GCTCTGGCCA GCTGTACACG CTGCCTAAAC 
AAGATCATGA AAACCAGAGT CTGCCGCCGC CTCCGCCGAG CCAACCGGGT CCTTCGGGCA 
CGCTGCCGTC GGGCAGTGAA GTCCAATGCC TGCTACTGGG CTGTGCTGTT GCTCGTCTTC 
CTCAACACGT TGACCATCGC CTCTGAGCAC CACGGGCAGC CTGTGTGGCT CACCCAGATC 
CAGGAGTATG CCAACAAAGT GTTGCTCTGT CTGTTCACGG TGGAGATGCT TCTCAAATTG 
TACGGTCTGG GCCCCTCTGC CTATGTGTCT TCCTTCTTCA ACCGCTTTGA CTGCTTTGTG 
GTCTGTGGGG GCATCCTAGA GACCACCTTG GTGGAGGTGG GTGCCATGCA GCCCTTGGGC 
ATCTCAGTGC TCCGATGTGT GCGCCTCCTC AGGATCTTTA AGGTCACCAG ACACTGGGCT 
TCTCTGAGCA ATCTGGTGGC ATCCCTGCTC AATTCAATGA AATCCATCGC ATCCTTGCTG 
CTTCTCCTCT TCCTCTTCAT CATTATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACCAAG CGAAGCACCT TTGACACGTT CCCCCAGGCC 
CTCCTCACTG TCTTTCAGAT CCTGACAGGT GAGGACTGGA ACGTGGTCAT GTATGATGGT 
ATCATGGCAT ATGGTGGCCC CTTCTTCCCA GGAATGTTGG TGTGCATCTA TTTCATCATT 
CTCTTCATCT GTGGCAACTA CATCCTGTTG AACGTGTTTC TTGCCATTGC TGTGGACAAC 
CTGGCCAGTG GAGATGCAGG CACTGCCAAG GACAAGGGCG GGGAGAAGAG CAATGAGAAG 
GATCTCCCAC AGGAGAATGA AGGCCTGGTG CCTGGTGTGG AGAAAGAGGA AGAGGAGGGT 
GCAAGGAGGG AAGGAGCAGA CATGGAGGAG GAGGAGGAGG AGGAAGAAGA GGAAGAAGAG 
GAAGAAGAGG AAGAGGGTGC AGGGGGTGTG GAACTCCTGC AGGAAGTTGT ACCCAAGGAG 
AAGGTGGTAC CCATCCCTGA GGGCAGCGCC TTCTTCTGCC TCAGCCAAAC CAACCCGCTG 
AGGAAGGGCT GCCACACCCT CATCCACCAT CATGTCTTCA CCAATCTTAT CCTGGTGTTC 
ATCATCCTCA GCAGTGTGTC CCTGGCCGCT GAGGACCCCA TCCGAGCCCA CTCCTTCCGC 
AACCATATTC TGGGTTACTT CGATTATGCC TTCACCTCCA TTTTCACTGT GGAGATTCTA 
CTAAAGATGA CAGTGTTTGG GGCCTTCCTG CACCGCGGCT CCTTCTGCCG TAGCTGGTTT 
AATATGTTGG ATCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTTGGCAT CCACTCCAGC 
GCCATCTCGG TGGTGAAGAT TCTGCGAGTA CTCCGAGTAC TGCGGCCCCT CCGAGCCATC 
AACAGGGCCA AGGGACTCAA GCATGTGGTG CAGTGTGTAT TTGTGGCCAT CCGGACCATC 
GGAAACATCA TGATTGTCAC CACACTTCTG CAATTTATGT TCGCCTGCAT CGGGGTGCAG 
CTCTTCAAGG GGAAATTCTA CACCTGCACG GACGAGGCCA AACACACCCC TCAAGAATGC 
AAGGGCTCCT TCCTGGTATA CCCAGATGGA GACGTGTCAC GGCCCCTGGT CCGGGAGCGG 
CTCTGGGTCA ACAGTGATTT CAACTTTGAC AATGTCCTTT CAGCCATGAT GGCCCTGTTC 
ACTGTCTCCA CCTTTGAAGG CTGGCCTGCA CTGCTATACA AGGCCATCGA TGCATATGCA 
GAGGACCACG GCCCCATCTA TAATTACCGT GTGGAGATCT CAGTGTTCTT CATTGTCTAC 
ATCATCATCA TTGCGTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CATCACTTTC 
CGTGCCCAGG GCGAGCAGGA GTACCAAAAC TGTGAGCTGG ACAAGAACCA GCGTCAATGT 
GTGGAATATG CCCTCAAGGC CCAGCCACTC CGCCGTTACA TCCCCAAGAA CCCGCATCAG 
TATCGTGTGT GGGCCACTGT GAACTCTGCT GCCTTTGAGT ACCTGATGTT CCTGCTCATC 
CTGCTCAACA CAGTTGCCCT AGCCATGCAG CACTATGAGC AGACTGCTCC CTTCAACTAT 
GCCATGGACA TCCTCAACAT GGTCTTCACT GGCCTCTTCA CTATTGAGAT GGTGCTCAAA 
ATCATCGCCT TCAAGCCCAA GCATTACTTC ACTGATGCCT GGAACACGTT TGACGCTCTT 
ATTGTGGTGG GCAGCATAGT GGATATTGCC GTCACTGAAG TCAATAATGG TGGCCACCTT 
GGCGAGAGCT CTGAGGACAG CTCCCGCATT TCCATTACCT TCTTTCGCCT CTTCCGAGTT 
ATGCGGCTGG TCAAGCTTCT CAGTAAGGGT GAAGGGATCC GCACATTGCT CTGGACATTC 
ATCAAGTCCT TCCAGGCCTT GCCCTATGTG GCTCTTCTCA TCGCAATGAT ATTCTTCATC 
TATGCCGTCA TTGGCATGCA GATGTTCGGC AAGGTGGCTC TTCAGGATGG CACACAGATA 
AACCGAAACA ACAACTTCCA GACCTTTCCA CAGGCTGTGC TGCTTCTGTT CAGGTGTGCC 
ACTGGTGAGG CATGGCAGGA GATAATGCTT GCCAGCCTTC CCGGAAATCG GTGTGATCCT 
GAGTCTGACT TCGGCCCTGG TGAAGAGTTT ACCTGTGGTA GCAATTTTGC CATCGCCTAT 
TTCATCAGCT TCTTCATGCT CTGTGCCTTC CTGATCATAA ATCTCTTTGT GGCTGTGATC 
ATGGACAACT TTGATTATCT CACCAGAGAT TGGTCCATCC TGGGCCCCCA TCACCTTGAT 
GAATTCAAGA GGATCTGGTC TGAATATGAC CCTGGGGCCA AGGGCCGCAT CAAACACTTG 
GATGTGGTTG CCCTGCTGAG ACGTATCCAG CCCCCTCTGG GATTTGGGAA GCTGTGCCCA 
CACCGAGTGG CCTGCAAGAG ACTTGTGGCA ATGAACATGC CCCTCAACTC AGATGGGACG 
GTGACATTCA ACGCCACACT CTTTGCCCTG GTCCGGACAT CCCTGAAGAT CAAAACAGAA 
GGGAACCTGG AGCAAGCCAA CCAGGAGCTG CGGATTGTCA TCAAAAAGAT CTGGAAGCGG 
ATGAAACAGA AGCTGCTAGA TGAGGTCATC CCCCCACCAG ACGAGGAGGA GGTCACCGTG 
GGCAAATTCT ACGCCACATT TCTGATCCAG GACTATTTCC GCAAATTCCG GCGGAGGAAA 
GAAAAAGGGC TACTAGGCAA CGACGCCGCC CCTAGCACCT CTTCCGCCCT TCAGGCTGGT 
CTGCGGAGCC TGCAGGACTT GGGTCCTGAG ATGCGGCAGG CCCTCACCTG TGACACAGAG 
GAGGAGGAAG AAGAGGGGCA GGAGGGAGTG GAGGAGGAAG ATGAAAAGGA CTTGGAAACT 
AACAAAGCCA CGATGGTCTC CCAGCCCTCA GCTCGCCGGG GCTCCGGGAT TTCTGTGTCT 
CTGCCTGTCG GGGACAGACT TCCAGATTCA CTCTCCTTTG GGCCCAGTGA TGATGACAGG 
GGGACTCCCA CCTCCAGTCA GCCCAGTGTG CCCCAGGCTG GATCCAACAC CCACAGGAGA 
GGCTCTGGGG CTCTCATTTT CACCATCCCA GAAGAAGGAA ATTCTCAGCC CAAGGGAACC 
AAAGGGCAAA ACAAGCAAGA TGAGGATGAG GAAGTCCCTG ATCGGCTTTC CTACCTAGAT 
GAGCAGGCAG GGACTCCCCC GTGCTCAGTC CTTTTGCCAC CTCACAGAGC TCAGAGATAC 
ATGGATGGGC ACCTGGTACC ACGCCGCCGT CTGCTGCCCC CCACACCTGC AGGTCGGAAG 
CCCTCCTTCA CCATCCAGTG TCTGCAGCGC CAGGGCAGTT GTGAGGATTT ACCCATCCCA 
GGCACCTATC ATCGTGGGCG AAATTCAGGG CCCAATAGGG CTCAGGGTTC CTGGGCAACA 
CCACCTCAGC GGGGTCGGCT CCTGTATGCC CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG 
GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC CCACTGCGCA CCTTCACCTG TCTGCACGTG 
CCTGGAACCC ACTCGGACCC CAGCCATGGG AAGAGGGGCA GTGCCGACAG CTTGGTGGAG 
GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC TTTGCTCGAG ACCCACGTTT CGTGGCCCTG 
GCCAAGCAGG AGATTGCAGA TGCGTGTCGC CTGACGCTGG ATGAGATGGA CAATGCTGCC 
AGTGACCTGC TGGCACAGGG AACCAGCTCT CTCTATAGCG ACGAGGAGTC CATCCTCTCC 
CGCTTCGATG AGGAGGACTT GGGAGACGAG ATGGCCTGCG TCCACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005174.2
CDS	63..5996
Translation	MSESEGGKDTTPEPSPANGAGPGPEWGLCPGPPAVEGESSGASGLGTPKRRNQHSKHKTVAVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNLGSMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_005183.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005183.3

ORF Insert Sequence:

ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT 
GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG 
GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT 
CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG 
ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG 
GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC 
GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT 
GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA 
TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC 
GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG 
CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA 
GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG 
AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC 
CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC 
GCCGATGACA ACCTTGGTTC TATGGCTGAA GAGGGCCGGG CGGGCCATCG GCCACAGCTG 
GCCGAGCTGA CCAATAGGAG GCGTGGACGT CTGCGCTGGT TCAGTCATTC TACTCGCTCC 
ACACACTCCA CCAGCAGCCA TGCCAGCCTC CCAGCCAGTG ACACCGGTTC CATGACAGAG 
ACCCAAGGCG ATGAGGATGA GGAGGAGGGG GCTCTGGCCA GCTGTACACG CTGCCTAAAC 
AAGATCATGA AAACCAGAGT CTGCCGCCGC CTCCGCCGAG CCAACCGGGT CCTTCGGGCA 
CGCTGCCGTC GGGCAGTGAA GTCCAATGCC TGCTACTGGG CTGTGCTGTT GCTCGTCTTC 
CTCAACACGT TGACCATCGC CTCTGAGCAC CACGGGCAGC CTGTGTGGCT CACCCAGATC 
CAGGAGTATG CCAACAAAGT GTTGCTCTGT CTGTTCACGG TGGAGATGCT TCTCAAATTG 
TACGGTCTGG GCCCCTCTGC CTATGTGTCT TCCTTCTTCA ACCGCTTTGA CTGCTTTGTG 
GTCTGTGGGG GCATCCTAGA GACCACCTTG GTGGAGGTGG GTGCCATGCA GCCCTTGGGC 
ATCTCAGTGC TCCGATGTGT GCGCCTCCTC AGGATCTTTA AGGTCACCAG ACACTGGGCT 
TCTCTGAGCA ATCTGGTGGC ATCCCTGCTC AATTCAATGA AATCCATCGC ATCCTTGCTG 
CTTCTCCTCT TCCTCTTCAT CATTATCTTC TCCCTGCTTG GCATGCAGCT GTTTGGGGGC 
AAGTTCAACT TTGACCAGAC CCACACCAAG CGAAGCACCT TTGACACGTT CCCCCAGGCC 
CTCCTCACTG TCTTTCAGAT CCTGACAGGT GAGGACTGGA ACGTGGTCAT GTATGATGGT 
ATCATGGCAT ATGGTGGCCC CTTCTTCCCA GGAATGTTGG TGTGCATCTA TTTCATCATT 
CTCTTCATCT GTGGCAACTA CATCCTGTTG AACGTGTTTC TTGCCATTGC TGTGGACAAC 
CTGGCCAGTG GAGATGCAGG CACTGCCAAG GACAAGGGCG GGGAGAAGAG CAATGAGAAG 
GATCTCCCAC AGGAGAATGA AGGCCTGGTG CCTGGTGTGG AGAAAGAGGA AGAGGAGGGT 
GCAAGGAGGG AAGGAGCAGA CATGGAGGAG GAGGAGGAGG AGGAAGAAGA GGAAGAAGAG 
GAAGAAGAGG AAGAGGGTGC AGGGGGTGTG GAACTCCTGC AGGAAGTTGT ACCCAAGGAG 
AAGGTGGTAC CCATCCCTGA GGGCAGCGCC TTCTTCTGCC TCAGCCAAAC CAACCCGCTG 
AGGAAGGGCT GCCACACCCT CATCCACCAT CATGTCTTCA CCAATCTTAT CCTGGTGTTC 
ATCATCCTCA GCAGTGTGTC CCTGGCCGCT GAGGACCCCA TCCGAGCCCA CTCCTTCCGC 
AACCATATTC TGGGTTACTT CGATTATGCC TTCACCTCCA TTTTCACTGT GGAGATTCTA 
CTAAAGATGA CAGTGTTTGG GGCCTTCCTG CACCGCGGCT CCTTCTGCCG TAGCTGGTTT 
AATATGTTGG ATCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTTGGCAT CCACTCCAGC 
GCCATCTCGG TGGTGAAGAT TCTGCGAGTA CTCCGAGTAC TGCGGCCCCT CCGAGCCATC 
AACAGGGCCA AGGGACTCAA GCATGTGGTG CAGTGTGTAT TTGTGGCCAT CCGGACCATC 
GGAAACATCA TGATTGTCAC CACACTTCTG CAATTTATGT TCGCCTGCAT CGGGGTGCAG 
CTCTTCAAGG GGAAATTCTA CACCTGCACG GACGAGGCCA AACACACCCC TCAAGAATGC 
AAGGGCTCCT TCCTGGTATA CCCAGATGGA GACGTGTCAC GGCCCCTGGT CCGGGAGCGG 
CTCTGGGTCA ACAGTGATTT CAACTTTGAC AATGTCCTTT CAGCCATGAT GGCCCTGTTC 
ACTGTCTCCA CCTTTGAAGG CTGGCCTGCA CTGCTATACA AGGCCATCGA TGCATATGCA 
GAGGACCACG GCCCCATCTA TAATTACCGT GTGGAGATCT CAGTGTTCTT CATTGTCTAC 
ATCATCATCA TTGCGTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CATCACTTTC 
CGTGCCCAGG GCGAGCAGGA GTACCAAAAC TGTGAGCTGG ACAAGAACCA GCGTCAATGT 
GTGGAATATG CCCTCAAGGC CCAGCCACTC CGCCGTTACA TCCCCAAGAA CCCGCATCAG 
TATCGTGTGT GGGCCACTGT GAACTCTGCT GCCTTTGAGT ACCTGATGTT CCTGCTCATC 
CTGCTCAACA CAGTTGCCCT AGCCATGCAG CACTATGAGC AGACTGCTCC CTTCAACTAT 
GCCATGGACA TCCTCAACAT GGTCTTCACT GGCCTCTTCA CTATTGAGAT GGTGCTCAAA 
ATCATCGCCT TCAAGCCCAA GCATTACTTC ACTGATGCCT GGAACACGTT TGACGCTCTT 
ATTGTGGTGG GCAGCATAGT GGATATTGCC GTCACTGAAG TCAATAATGG TGGCCACCTT 
GGCGAGAGCT CTGAGGACAG CTCCCGCATT TCCATTACCT TCTTTCGCCT CTTCCGAGTT 
ATGCGGCTGG TCAAGCTTCT CAGTAAGGGT GAAGGGATCC GCACATTGCT CTGGACATTC 
ATCAAGTCCT TCCAGGCCTT GCCCTATGTG GCTCTTCTCA TCGCAATGAT ATTCTTCATC 
TATGCCGTCA TTGGCATGCA GATGTTCGGC AAGGTGGCTC TTCAGGATGG CACACAGATA 
AACCGAAACA ACAACTTCCA GACCTTTCCA CAGGCTGTGC TGCTTCTGTT CAGGTGTGCC 
ACTGGTGAGG CATGGCAGGA GATAATGCTT GCCAGCCTTC CCGGAAATCG GTGTGATCCT 
GAGTCTGACT TCGGCCCTGG TGAAGAGTTT ACCTGTGGTA GCAATTTTGC CATCGCCTAT 
TTCATCAGCT TCTTCATGCT CTGTGCCTTC CTGATCATAA ATCTCTTTGT GGCTGTGATC 
ATGGACAACT TTGATTATCT CACCAGAGAT TGGTCCATCC TGGGCCCCCA TCACCTTGAT 
GAATTCAAGA GGATCTGGTC TGAATATGAC CCTGGGGCCA AGGGCCGCAT CAAACACTTG 
GATGTGGTTG CCCTGCTGAG ACGTATCCAG CCCCCTCTGG GATTTGGGAA GCTGTGCCCA 
CACCGAGTGG CCTGCAAGAG ACTTGTGGCA ATGAACATGC CCCTCAACTC AGATGGGACG 
GTGACATTCA ACGCCACACT CTTTGCCCTG GTCCGGACAT CCCTGAAGAT CAAAACAGAA 
GGGAACCTGG AGCAAGCCAA CCAGGAGCTG CGGATTGTCA TCAAAAAGAT CTGGAAGCGG 
ATGAAACAGA AGCTGCTAGA TGAGGTCATC CCCCCACCAG ACGAGGAGGA GGTCACCGTG 
GGCAAATTCT ACGCCACATT TCTGATCCAG GACTATTTCC GCAAATTCCG GCGGAGGAAA 
GAAAAAGGGC TACTAGGCAA CGACGCCGCC CCTAGCACCT CTTCCGCCCT TCAGGCTGGT 
CTGCGGAGCC TGCAGGACTT GGGTCCTGAG ATGCGGCAGG CCCTCACCTG TGACACAGAG 
GAGGAGGAAG AAGAGGGGCA GGAGGGAGTG GAGGAGGAAG ATGAAAAGGA CTTGGAAACT 
AACAAAGCCA CGATGGTCTC CCAGCCCTCA GCTCGCCGGG GCTCCGGGAT TTCTGTGTCT 
CTGCCTGTCG GGGACAGACT TCCAGATTCA CTCTCCTTTG GGCCCAGTGA TGATGACAGG 
GGGACTCCCA CCTCCAGTCA GCCCAGTGTG CCCCAGGCTG GATCCAACAC CCACAGGAGA 
GGCTCTGGGG CTCTCATTTT CACCATCCCA GAAGAAGGAA ATTCTCAGCC CAAGGGAACC 
AAAGGGCAAA ACAAGCAAGA TGAGGATGAG GAAGTCCCTG ATCGGCTTTC CTACCTAGAT 
GAGCAGGCAG GGACTCCCCC GTGCTCAGTC CTTTTGCCAC CTCACAGAGC TCAGAGATAC 
ATGGATGGGC ACCTGGTACC ACGCCGCCGT CTGCTGCCCC CCACACCTGC AGGTCGGAAG 
CCCTCCTTCA CCATCCAGTG TCTGCAGCGC CAGGGCAGTT GTGAGGATTT ACCCATCCCA 
GGCACCTATC ATCGTGGGCG AAATTCAGGG CCCAATAGGG CTCAGGGTTC CTGGGCAACA 
CCACCTCAGC GGGGTCGGCT CCTGTATGCC CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG 
GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC CCACTGCGCA CCTTCACCTG TCTGCACGTG 
CCTGGAACCC ACTCGGACCC CAGCCATGGG AAGAGGGGCA GTGCCGACAG CTTGGTGGAG 
GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC TTTGCTCGAG ACCCACGTTT CGTGGCCCTG 
GCCAAGCAGG AGATTGCAGA TGCGTGTCGC CTGACGCTGG ATGAGATGGA CAATGCTGCC 
AGTGACCTGC TGGCACAGGG AACCAGCTCT CTCTATAGCG ACGAGGAGTC CATCCTCTCC 
CGCTTCGATG AGGAGGACTT GGGAGACGAG ATGGCCTGCG TCCACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( XM_017029836.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885325.1 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X1, mRNA.

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CloneID	OHu99472
Clone ID Related Accession (Same CDS sequence)	XM_017029836.1
Accession Version	XM_017029836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGTGT TTGGGGCCTT CCTGCACCGC GGCTCCTTCT GCCGTAGCTG GTTTAATATG 
TTGGATCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTTG GCATCCACTC CAGCGCCATC 
TCGGTGGTGA AGATTCTGCG AGTACTCCGA GTACTGCGGC CCCTCCGAGC CATCAACAGG 
GCCAAGGGAC TCAAGCATGT GGTGCAGTGT GTATTTGTGG CCATCCGGAC CATCGGAAAC 
ATCATGATTG TCACCACACT TCTGCAATTT ATGTTCGCCT GCATCGGGGT GCAGCTCTTC 
AAGGGGAAAT TCTACACCTG CACGGACGAG GCCAAACACA CCCCTCAAGA ATGCAAGGGC 
TCCTTCCTGG TATACCCAGA TGGAGACGTG TCACGGCCCC TGGTCCGGGA GCGGCTCTGG 
GTCAACAGTG ATTTCAACTT TGACAATGTC CTTTCAGCCA TGATGGCCCT GTTCACTGTC 
TCCACCTTTG AAGGCTGGCC TGCACTGCTA TACAAGGCCA TCGATGCATA TGCAGAGGAC 
CACGGCCCCA TCTATAATTA CCGTGTGGAG ATCTCAGTGT TCTTCATTGT CTACATCATC 
ATCATTGCGT TCTTCATGAT GAACATCTTC GTGGGCTTCG TCATCATCAC TTTCCGTGCC 
CAGGGCGAGC AGGAGTACCA AAACTGTGAG CTGGACAAGA ACCAGCGTCA ATGTGTGGAA 
TATGCCCTCA AGGCCCAGCC ACTCCGCCGT TACATCCCCA AGAACCCGCA TCAGTATCGT 
GTGTGGGCCA CTGTGAACTC TGCTGCCTTT GAGTACCTGA TGTTCCTGCT CATCCTGCTC 
AACACAGTTG CCCTAGCCAT GCAGCACTAT GAGCAGACTG CTCCCTTCAA CTATGCCATG 
GACATCCTCA ACATGGTCTT CACTGGCCTC TTCACTATTG AGATGGTGCT CAAAATCATC 
GCCTTCAAGC CCAAGCATTA CTTCACTGAT GCCTGGAACA CGTTTGACGC TCTTATTGTG 
GTGGGCAGCA TAGTGGATAT TGCCGTCACT GAAGTCAATA GCTCTGAGGA CAGCTCCCGC 
ATTTCCATTA CCTTCTTTCG CCTCTTCCGA GTTATGCGGC TGGTCAAGCT TCTCAGTAAG 
GGTGAAGGGA TCCGCACATT GCTCTGGACA TTCATCAAGT CCTTCCAGGC CTTGCCCTAT 
GTGGCTCTTC TCATCGCAAT GATATTCTTC ATCTATGCCG TCATTGGCAT GCAGATGTTC 
GGCAAGGTGG CTCTTCAGGA TGGCACACAG ATAAACCGAA ACAACAACTT CCAGACCTTT 
CCACAGGCTG TGCTGCTTCT GTTCAGGTGT GCCACTGGTG AGGCATGGCA GGAGATAATG 
CTTGCCAGCC TTCCCGGAAA TCGGTGTGAT CCTGAGTCTG ACTTCGGCCC TGGTGAAGAG 
TTTACCTGTG GTAGCAATTT TGCCATCGCC TATTTCATCA GCTTCTTCAT GCTCTGTGCC 
TTCCTGATCA TAAATCTCTT TGTGGCTGTG ATCATGGACA ACTTTGATTA TCTCACCAGA 
GATTGGTCCA TCCTGGGCCC CCATCACCTT GATGAATTCA AGAGGATCTG GTCTGAATAT 
GACCCTGGGG CCAAGGGCCG CATCAAACAC TTGGATGTGG TTGCCCTGCT GAGACGTATC 
CAGCCCCCTC TGGGATTTGG GAAGCTGTGC CCACACCGAG TGGCCTGCAA GAGACTTGTG 
GCAATGAACA TGCCCCTCAA CTCAGATGGG ACGGTGACAT TCAACGCCAC ACTCTTTGCC 
CTGGTCCGGA CATCCCTGAA GATCAAAACA GAAGGGAACC TGGAGCAAGC CAACCAGGAG 
CTGCGGATTG TCATCAAAAA GATCTGGAAG CGGATGAAAC AGAAGCTGCT AGATGAGGTC 
ATCCCCCCAC CAGACGAGGA GGAGGTCACC GTGGGCAAAT TCTACGCCAC ATTTCTGATC 
CAGGACTATT TCCGCAAATT CCGGCGGAGG AAAGAAAAAG GGCTACTAGG CAACGACGCC 
GCCCCTAGCA CCTCTTCCGC CCTTCAGGCT GGTCTGCGGA GCCTGCAGGA CTTGGGTCCT 
GAGATGCGGC AGGCCCTCAC CTGTGACACA GAGGAGGAGG AAGAAGAGGG GCAGGAGGGA 
GTGGAGGAGG AAGATGAAAA GGACTTGGAA ACTAACAAAG CCACGATGGT CTCCCAGCCC 
TCAGCTCGCC GGGGCTCCGG GATTTCTGTG TCTCTGCCTG TCGGGGACAG ACTTCCAGAT 
TCACTCTCCT TTGGGCCCAG TGATGATGAC AGGGGGACTC CCACCTCCAG TCAGCCCAGT 
GTGCCCCAGG CTGGATCCAA CACCCACAGG AGAGGCTCTG GGGCTCTCAT TTTCACCATC 
CCAGAAGAAG GAAATTCTCA GCCCAAGGGA ACCAAAGGGC AAAACAAGCA AGATGAGGAT 
GAGGAAGTCC CTGATCGAGC TCAGAGATAC ATGGATGGGC ACCTGGTACC ACGCCGCCGT 
CTGCTGCCCC CCACACCTGC AGGTCGGAAG CCCTCCTTCA CCATCCAGTG TCTGCAGCGC 
CAGGGCAGTT GTGAGGATTT ACCCATCCCA GGCACCTATC ATCGTGGGCG AAATTCAGGG 
CCCAATAGGG CTCAGGGTTC CTGGGCAACA CCACCTCAGC GGGGTCGGCT CCTGTATGCC 
CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC 
CCACTGCGCA CCTTCACCTG TCTGCACGTG CCTGGAACCC ACTCGGACCC CAGCCATGGG 
AAGAGGGGCA GTGCCGACAG CTTGGTGGAG GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC 
TTTGCTCGAG ACCCACGTTT CGTGGCCCTG GCCAAGCAGG AGATTGCAGA TGCGTGTCGC 
CTGACGCTGG ATGAGATGGA CAATGCTGCC AGTGACCTGC TGGCACAGGG AACCAGCTCT 
CTCTATAGCG ACGAGGAGTC CATCCTCTCC CGCTTCGATG AGGAGGACTT GGGAGACGAG 
ATGGCCTGCG TCCACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885325.1
CDS	86..3169
Translation	MTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNSSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_017029836.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029836.1

ORF Insert Sequence:

ATGACAGTGT TTGGGGCCTT CCTGCACCGC GGCTCCTTCT GCCGTAGCTG GTTTAATATG 
TTGGATCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTTG GCATCCACTC CAGCGCCATC 
TCGGTGGTGA AGATTCTGCG AGTACTCCGA GTACTGCGGC CCCTCCGAGC CATCAACAGG 
GCCAAGGGAC TCAAGCATGT GGTGCAGTGT GTATTTGTGG CCATCCGGAC CATCGGAAAC 
ATCATGATTG TCACCACACT TCTGCAATTT ATGTTCGCCT GCATCGGGGT GCAGCTCTTC 
AAGGGGAAAT TCTACACCTG CACGGACGAG GCCAAACACA CCCCTCAAGA ATGCAAGGGC 
TCCTTCCTGG TATACCCAGA TGGAGACGTG TCACGGCCCC TGGTCCGGGA GCGGCTCTGG 
GTCAACAGTG ATTTCAACTT TGACAATGTC CTTTCAGCCA TGATGGCCCT GTTCACTGTC 
TCCACCTTTG AAGGCTGGCC TGCACTGCTA TACAAGGCCA TCGATGCATA TGCAGAGGAC 
CACGGCCCCA TCTATAATTA CCGTGTGGAG ATCTCAGTGT TCTTCATTGT CTACATCATC 
ATCATTGCGT TCTTCATGAT GAACATCTTC GTGGGCTTCG TCATCATCAC TTTCCGTGCC 
CAGGGCGAGC AGGAGTACCA AAACTGTGAG CTGGACAAGA ACCAGCGTCA ATGTGTGGAA 
TATGCCCTCA AGGCCCAGCC ACTCCGCCGT TACATCCCCA AGAACCCGCA TCAGTATCGT 
GTGTGGGCCA CTGTGAACTC TGCTGCCTTT GAGTACCTGA TGTTCCTGCT CATCCTGCTC 
AACACAGTTG CCCTAGCCAT GCAGCACTAT GAGCAGACTG CTCCCTTCAA CTATGCCATG 
GACATCCTCA ACATGGTCTT CACTGGCCTC TTCACTATTG AGATGGTGCT CAAAATCATC 
GCCTTCAAGC CCAAGCATTA CTTCACTGAT GCCTGGAACA CGTTTGACGC TCTTATTGTG 
GTGGGCAGCA TAGTGGATAT TGCCGTCACT GAAGTCAATA GCTCTGAGGA CAGCTCCCGC 
ATTTCCATTA CCTTCTTTCG CCTCTTCCGA GTTATGCGGC TGGTCAAGCT TCTCAGTAAG 
GGTGAAGGGA TCCGCACATT GCTCTGGACA TTCATCAAGT CCTTCCAGGC CTTGCCCTAT 
GTGGCTCTTC TCATCGCAAT GATATTCTTC ATCTATGCCG TCATTGGCAT GCAGATGTTC 
GGCAAGGTGG CTCTTCAGGA TGGCACACAG ATAAACCGAA ACAACAACTT CCAGACCTTT 
CCACAGGCTG TGCTGCTTCT GTTCAGGTGT GCCACTGGTG AGGCATGGCA GGAGATAATG 
CTTGCCAGCC TTCCCGGAAA TCGGTGTGAT CCTGAGTCTG ACTTCGGCCC TGGTGAAGAG 
TTTACCTGTG GTAGCAATTT TGCCATCGCC TATTTCATCA GCTTCTTCAT GCTCTGTGCC 
TTCCTGATCA TAAATCTCTT TGTGGCTGTG ATCATGGACA ACTTTGATTA TCTCACCAGA 
GATTGGTCCA TCCTGGGCCC CCATCACCTT GATGAATTCA AGAGGATCTG GTCTGAATAT 
GACCCTGGGG CCAAGGGCCG CATCAAACAC TTGGATGTGG TTGCCCTGCT GAGACGTATC 
CAGCCCCCTC TGGGATTTGG GAAGCTGTGC CCACACCGAG TGGCCTGCAA GAGACTTGTG 
GCAATGAACA TGCCCCTCAA CTCAGATGGG ACGGTGACAT TCAACGCCAC ACTCTTTGCC 
CTGGTCCGGA CATCCCTGAA GATCAAAACA GAAGGGAACC TGGAGCAAGC CAACCAGGAG 
CTGCGGATTG TCATCAAAAA GATCTGGAAG CGGATGAAAC AGAAGCTGCT AGATGAGGTC 
ATCCCCCCAC CAGACGAGGA GGAGGTCACC GTGGGCAAAT TCTACGCCAC ATTTCTGATC 
CAGGACTATT TCCGCAAATT CCGGCGGAGG AAAGAAAAAG GGCTACTAGG CAACGACGCC 
GCCCCTAGCA CCTCTTCCGC CCTTCAGGCT GGTCTGCGGA GCCTGCAGGA CTTGGGTCCT 
GAGATGCGGC AGGCCCTCAC CTGTGACACA GAGGAGGAGG AAGAAGAGGG GCAGGAGGGA 
GTGGAGGAGG AAGATGAAAA GGACTTGGAA ACTAACAAAG CCACGATGGT CTCCCAGCCC 
TCAGCTCGCC GGGGCTCCGG GATTTCTGTG TCTCTGCCTG TCGGGGACAG ACTTCCAGAT 
TCACTCTCCT TTGGGCCCAG TGATGATGAC AGGGGGACTC CCACCTCCAG TCAGCCCAGT 
GTGCCCCAGG CTGGATCCAA CACCCACAGG AGAGGCTCTG GGGCTCTCAT TTTCACCATC 
CCAGAAGAAG GAAATTCTCA GCCCAAGGGA ACCAAAGGGC AAAACAAGCA AGATGAGGAT 
GAGGAAGTCC CTGATCGAGC TCAGAGATAC ATGGATGGGC ACCTGGTACC ACGCCGCCGT 
CTGCTGCCCC CCACACCTGC AGGTCGGAAG CCCTCCTTCA CCATCCAGTG TCTGCAGCGC 
CAGGGCAGTT GTGAGGATTT ACCCATCCCA GGCACCTATC ATCGTGGGCG AAATTCAGGG 
CCCAATAGGG CTCAGGGTTC CTGGGCAACA CCACCTCAGC GGGGTCGGCT CCTGTATGCC 
CCGCTGTTGT TGGTGGAAGA GGGCGCAGCG GGGGAGGGGT ACCTCGGCAG ATCCAGTGGC 
CCACTGCGCA CCTTCACCTG TCTGCACGTG CCTGGAACCC ACTCGGACCC CAGCCATGGG 
AAGAGGGGCA GTGCCGACAG CTTGGTGGAG GCTGTGCTTA TCTCAGAGGG TCTGGGCCTC 
TTTGCTCGAG ACCCACGTTT CGTGGCCCTG GCCAAGCAGG AGATTGCAGA TGCGTGTCGC 
CTGACGCTGG ATGAGATGGA CAATGCTGCC AGTGACCTGC TGGCACAGGG AACCAGCTCT 
CTCTATAGCG ACGAGGAGTC CATCCTCTCC CGCTTCGATG AGGAGGACTT GGGAGACGAG 
ATGGCCTGCG TCCACGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( NM_001256790.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243719.1 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA.

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CloneID	OHu21327
Clone ID Related Accession (Same CDS sequence)	NM_001256790.2 , NM_001256790.3
Accession Version	NM_001256790.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF201304.1, AF067227.1 and AC232271.2. On Aug 21, 2013 this sequence version replaced NM_001256790.1. Transcript Variant: This variant (3) uses two alternate splice sites in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF067227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC 
TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC 
AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG 
ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA 
CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG 
AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA 
GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC 
TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG 
ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT 
TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG 
CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG 
TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT 
GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC 
CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC 
GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG 
GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT 
GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT 
AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC 
AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG 
GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC 
AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA 
GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC 
AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC 
TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC 
CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA 
TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG 
GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC 
TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT 
CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT 
GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC 
AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT 
GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG 
AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA 
GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG 
GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC 
CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC 
ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT 
GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT 
TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG 
TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG 
AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA 
CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT 
GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA 
TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG 
GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT 
GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT 
GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC 
ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG 
CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC 
AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC 
ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT 
GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC 
AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG 
CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC 
ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AATGGTGGCC ACCTTGGCGA GAGCTCTGAG 
GACAGCTCCC GCATTTCCAT TACCTTCTTT CGCCTCTTCC GAGTTATGCG GCTGGTCAAG 
CTTCTCAGTA AGGGTGAAGG GATCCGCACA TTGCTCTGGA CATTCATCAA GTCCTTCCAG 
GCCTTGCCCT ATGTGGCTCT TCTCATCGCA ATGATATTCT TCATCTATGC CGTCATTGGC 
ATGCAGATGT TCGGCAAGGT GGCTCTTCAG GATGGCACAC AGATAAACCG AAACAACAAC 
TTCCAGACCT TTCCACAGGC TGTGCTGCTT CTGTTCAGGT GTGCCACTGG TGAGGCATGG 
CAGGAGATAA TGCTTGCCAG CCTTCCCGGA AATCGGTGTG ATCCTGAGTC TGACTTCGGC 
CCTGGTGAAG AGTTTACCTG TGGTAGCAAT TTTGCCATCG CCTATTTCAT CAGCTTCTTC 
ATGCTCTGTG CCTTCCTGAT CATAAATCTC TTTGTGGCTG TGATCATGGA CAACTTTGAT 
TATCTCACCA GAGATTGGTC CATCCTGGGC CCCCATCACC TTGATGAATT CAAGAGGATC 
TGGTCTGAAT ATGACCCTGG GGCCAAGGGC CGCATCAAAC ACTTGGATGT GGTTGCCCTG 
CTGAGACGTA TCCAGCCCCC TCTGGGATTT GGGAAGCTGT GCCCACACCG AGTGGCCTGC 
AAGAGACTTG TGGCAATGAA CATGCCCCTC AACTCAGATG GGACGGTGAC ATTCAACGCC 
ACACTCTTTG CCCTGGTCCG GACATCCCTG AAGATCAAAA CAGAAGGGAA CCTGGAGCAA 
GCCAACCAGG AGCTGCGGAT TGTCATCAAA AAGATCTGGA AGCGGATGAA ACAGAAGCTG 
CTAGATGAGG TCATCCCCCC ACCAGACGAG GAGGAGGTCA CCGTGGGCAA ATTCTACGCC 
ACATTTCTGA TCCAGGACTA TTTCCGCAAA TTCCGGCGGA GGAAAGAAAA AGGGCTACTA 
GGCAACGACG CCGCCCCTAG CACCTCTTCC GCCCTTCAGG CTGGTCTGCG GAGCCTGCAG 
GACTTGGGTC CTGAGATGCG GCAGGCCCTC ACCTGTGACA CAGAGGAGGA GGAAGAAGAG 
GGGCAGGAGG GAGTGGAGGA GGAAGATGAA AAGGACTTGG AAACTAACAA AGCCACGATG 
GTCTCCCAGC CCTCAGCTCG CCGGGGCTCC GGGATTTCTG TGTCTCTGCC TGTCGGGGAC 
AGACTTCCAG ATTCACTCTC CTTTGGGCCC AGTGATGATG ACAGGGGGAC TCCCACCTCC 
AGTCAGCCCA GTGTGCCCCA GGCTGGATCC AACACCCACA GGAGAGGCTC TGGGGCTCTC 
ATTTTCACCA TCCCAGAAGA AGGAAATTCT CAGCCCAAGG GAACCAAAGG GCAAAACAAG 
CAAGATGAGG ATGAGGAAGT CCCTGATCGG CTTTCCTACC TAGATGAGCA GGCAGGGACT 
CCCCCGTGCT CAGTCCTTTT GCCACCTCAC AGAGCTCAGA GATACATGGA TGGGCACCTG 
GTACCACGCC GCCGTCTGCT GCCCCCCACA CCTGCAGGTC GGAAGCCCTC CTTCACCATC 
CAGTGTCTGC AGCGCCAGGG CAGTTGTGAG GATTTACCCA TCCCAGGCAC CTATCATCGT 
GGGCGAAATT CAGGGCCCAA TAGGGCTCAG GGTTCCTGGG CAACACCACC TCAGCGGGGT 
CGGCTCCTGT ATGCCCCGCT GTTGTTGGTG GAAGAGGGCG CAGCGGGGGA GGGGTACCTC 
GGCAGATCCA GTGGCCCACT GCGCACCTTC ACCTGTCTGC ACGTGCCTGG AACCCACTCG 
GACCCCAGCC ATGGGAAGAG GGGCAGTGCC GACAGCTTGG TGGAGGCTGT GCTTATCTCA 
GAGGGTCTGG GCCTCTTTGC TCGAGACCCA CGTTTCGTGG CCCTGGCCAA GCAGGAGATT 
GCAGATGCGT GTCGCCTGAC GCTGGATGAG ATGGACAATG CTGCCAGTGA CCTGCTGGCA 
CAGGGAACCA GCTCTCTCTA TAGCGACGAG GAGTCCATCC TCTCCCGCTT CGATGAGGAG 
GACTTGGGAG ACGAGATGGC CTGCGTCCAC GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243719.1
CDS	63..5801
Translation	MSESEGGKGERILPSLQTLGASIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNLGSMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_001256790.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256790.2

ORF Insert Sequence:

ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC 
TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC 
AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG 
ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA 
CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG 
AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA 
GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC 
TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG 
ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT 
TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG 
CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG 
TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT 
GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC 
CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC 
GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG 
GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT 
GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT 
AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC 
AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG 
GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC 
AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA 
GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC 
AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC 
TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC 
CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA 
TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG 
GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC 
TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT 
CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT 
GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC 
AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT 
GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG 
AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA 
GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG 
GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC 
CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC 
ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT 
GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT 
TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG 
TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG 
AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA 
CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT 
GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA 
TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG 
GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT 
GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT 
GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC 
ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG 
CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC 
AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC 
ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT 
GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC 
AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG 
CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC 
ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AATGGTGGCC ACCTTGGCGA GAGCTCTGAG 
GACAGCTCCC GCATTTCCAT TACCTTCTTT CGCCTCTTCC GAGTTATGCG GCTGGTCAAG 
CTTCTCAGTA AGGGTGAAGG GATCCGCACA TTGCTCTGGA CATTCATCAA GTCCTTCCAG 
GCCTTGCCCT ATGTGGCTCT TCTCATCGCA ATGATATTCT TCATCTATGC CGTCATTGGC 
ATGCAGATGT TCGGCAAGGT GGCTCTTCAG GATGGCACAC AGATAAACCG AAACAACAAC 
TTCCAGACCT TTCCACAGGC TGTGCTGCTT CTGTTCAGGT GTGCCACTGG TGAGGCATGG 
CAGGAGATAA TGCTTGCCAG CCTTCCCGGA AATCGGTGTG ATCCTGAGTC TGACTTCGGC 
CCTGGTGAAG AGTTTACCTG TGGTAGCAAT TTTGCCATCG CCTATTTCAT CAGCTTCTTC 
ATGCTCTGTG CCTTCCTGAT CATAAATCTC TTTGTGGCTG TGATCATGGA CAACTTTGAT 
TATCTCACCA GAGATTGGTC CATCCTGGGC CCCCATCACC TTGATGAATT CAAGAGGATC 
TGGTCTGAAT ATGACCCTGG GGCCAAGGGC CGCATCAAAC ACTTGGATGT GGTTGCCCTG 
CTGAGACGTA TCCAGCCCCC TCTGGGATTT GGGAAGCTGT GCCCACACCG AGTGGCCTGC 
AAGAGACTTG TGGCAATGAA CATGCCCCTC AACTCAGATG GGACGGTGAC ATTCAACGCC 
ACACTCTTTG CCCTGGTCCG GACATCCCTG AAGATCAAAA CAGAAGGGAA CCTGGAGCAA 
GCCAACCAGG AGCTGCGGAT TGTCATCAAA AAGATCTGGA AGCGGATGAA ACAGAAGCTG 
CTAGATGAGG TCATCCCCCC ACCAGACGAG GAGGAGGTCA CCGTGGGCAA ATTCTACGCC 
ACATTTCTGA TCCAGGACTA TTTCCGCAAA TTCCGGCGGA GGAAAGAAAA AGGGCTACTA 
GGCAACGACG CCGCCCCTAG CACCTCTTCC GCCCTTCAGG CTGGTCTGCG GAGCCTGCAG 
GACTTGGGTC CTGAGATGCG GCAGGCCCTC ACCTGTGACA CAGAGGAGGA GGAAGAAGAG 
GGGCAGGAGG GAGTGGAGGA GGAAGATGAA AAGGACTTGG AAACTAACAA AGCCACGATG 
GTCTCCCAGC CCTCAGCTCG CCGGGGCTCC GGGATTTCTG TGTCTCTGCC TGTCGGGGAC 
AGACTTCCAG ATTCACTCTC CTTTGGGCCC AGTGATGATG ACAGGGGGAC TCCCACCTCC 
AGTCAGCCCA GTGTGCCCCA GGCTGGATCC AACACCCACA GGAGAGGCTC TGGGGCTCTC 
ATTTTCACCA TCCCAGAAGA AGGAAATTCT CAGCCCAAGG GAACCAAAGG GCAAAACAAG 
CAAGATGAGG ATGAGGAAGT CCCTGATCGG CTTTCCTACC TAGATGAGCA GGCAGGGACT 
CCCCCGTGCT CAGTCCTTTT GCCACCTCAC AGAGCTCAGA GATACATGGA TGGGCACCTG 
GTACCACGCC GCCGTCTGCT GCCCCCCACA CCTGCAGGTC GGAAGCCCTC CTTCACCATC 
CAGTGTCTGC AGCGCCAGGG CAGTTGTGAG GATTTACCCA TCCCAGGCAC CTATCATCGT 
GGGCGAAATT CAGGGCCCAA TAGGGCTCAG GGTTCCTGGG CAACACCACC TCAGCGGGGT 
CGGCTCCTGT ATGCCCCGCT GTTGTTGGTG GAAGAGGGCG CAGCGGGGGA GGGGTACCTC 
GGCAGATCCA GTGGCCCACT GCGCACCTTC ACCTGTCTGC ACGTGCCTGG AACCCACTCG 
GACCCCAGCC ATGGGAAGAG GGGCAGTGCC GACAGCTTGG TGGAGGCTGT GCTTATCTCA 
GAGGGTCTGG GCCTCTTTGC TCGAGACCCA CGTTTCGTGG CCCTGGCCAA GCAGGAGATT 
GCAGATGCGT GTCGCCTGAC GCTGGATGAG ATGGACAATG CTGCCAGTGA CCTGCTGGCA 
CAGGGAACCA GCTCTCTCTA TAGCGACGAG GAGTCCATCC TCTCCCGCTT CGATGAGGAG 
GACTTGGGAG ACGAGATGGC CTGCGTCCAC GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( NM_001256790.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243719.1 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA.

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CloneID	OHu21327
Clone ID Related Accession (Same CDS sequence)	NM_001256790.2 , NM_001256790.3
Accession Version	NM_001256790.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF201304.1, AF067227.1 and AC232271.2. On Jun 2, 2019 this sequence version replaced NM_001256790.2. Transcript Variant: This variant (3) uses two alternate splice sites in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF067227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC 
TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC 
AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG 
ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA 
CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG 
AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA 
GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC 
TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG 
ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT 
TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG 
CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG 
TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT 
GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC 
CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC 
GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG 
GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT 
GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT 
AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC 
AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG 
GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC 
AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA 
GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC 
AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC 
TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC 
CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA 
TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG 
GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC 
TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT 
CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT 
GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC 
AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT 
GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG 
AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA 
GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG 
GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC 
CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC 
ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT 
GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT 
TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG 
TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG 
AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA 
CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT 
GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA 
TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG 
GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT 
GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT 
GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC 
ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG 
CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC 
AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC 
ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT 
GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC 
AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG 
CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC 
ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AATGGTGGCC ACCTTGGCGA GAGCTCTGAG 
GACAGCTCCC GCATTTCCAT TACCTTCTTT CGCCTCTTCC GAGTTATGCG GCTGGTCAAG 
CTTCTCAGTA AGGGTGAAGG GATCCGCACA TTGCTCTGGA CATTCATCAA GTCCTTCCAG 
GCCTTGCCCT ATGTGGCTCT TCTCATCGCA ATGATATTCT TCATCTATGC CGTCATTGGC 
ATGCAGATGT TCGGCAAGGT GGCTCTTCAG GATGGCACAC AGATAAACCG AAACAACAAC 
TTCCAGACCT TTCCACAGGC TGTGCTGCTT CTGTTCAGGT GTGCCACTGG TGAGGCATGG 
CAGGAGATAA TGCTTGCCAG CCTTCCCGGA AATCGGTGTG ATCCTGAGTC TGACTTCGGC 
CCTGGTGAAG AGTTTACCTG TGGTAGCAAT TTTGCCATCG CCTATTTCAT CAGCTTCTTC 
ATGCTCTGTG CCTTCCTGAT CATAAATCTC TTTGTGGCTG TGATCATGGA CAACTTTGAT 
TATCTCACCA GAGATTGGTC CATCCTGGGC CCCCATCACC TTGATGAATT CAAGAGGATC 
TGGTCTGAAT ATGACCCTGG GGCCAAGGGC CGCATCAAAC ACTTGGATGT GGTTGCCCTG 
CTGAGACGTA TCCAGCCCCC TCTGGGATTT GGGAAGCTGT GCCCACACCG AGTGGCCTGC 
AAGAGACTTG TGGCAATGAA CATGCCCCTC AACTCAGATG GGACGGTGAC ATTCAACGCC 
ACACTCTTTG CCCTGGTCCG GACATCCCTG AAGATCAAAA CAGAAGGGAA CCTGGAGCAA 
GCCAACCAGG AGCTGCGGAT TGTCATCAAA AAGATCTGGA AGCGGATGAA ACAGAAGCTG 
CTAGATGAGG TCATCCCCCC ACCAGACGAG GAGGAGGTCA CCGTGGGCAA ATTCTACGCC 
ACATTTCTGA TCCAGGACTA TTTCCGCAAA TTCCGGCGGA GGAAAGAAAA AGGGCTACTA 
GGCAACGACG CCGCCCCTAG CACCTCTTCC GCCCTTCAGG CTGGTCTGCG GAGCCTGCAG 
GACTTGGGTC CTGAGATGCG GCAGGCCCTC ACCTGTGACA CAGAGGAGGA GGAAGAAGAG 
GGGCAGGAGG GAGTGGAGGA GGAAGATGAA AAGGACTTGG AAACTAACAA AGCCACGATG 
GTCTCCCAGC CCTCAGCTCG CCGGGGCTCC GGGATTTCTG TGTCTCTGCC TGTCGGGGAC 
AGACTTCCAG ATTCACTCTC CTTTGGGCCC AGTGATGATG ACAGGGGGAC TCCCACCTCC 
AGTCAGCCCA GTGTGCCCCA GGCTGGATCC AACACCCACA GGAGAGGCTC TGGGGCTCTC 
ATTTTCACCA TCCCAGAAGA AGGAAATTCT CAGCCCAAGG GAACCAAAGG GCAAAACAAG 
CAAGATGAGG ATGAGGAAGT CCCTGATCGG CTTTCCTACC TAGATGAGCA GGCAGGGACT 
CCCCCGTGCT CAGTCCTTTT GCCACCTCAC AGAGCTCAGA GATACATGGA TGGGCACCTG 
GTACCACGCC GCCGTCTGCT GCCCCCCACA CCTGCAGGTC GGAAGCCCTC CTTCACCATC 
CAGTGTCTGC AGCGCCAGGG CAGTTGTGAG GATTTACCCA TCCCAGGCAC CTATCATCGT 
GGGCGAAATT CAGGGCCCAA TAGGGCTCAG GGTTCCTGGG CAACACCACC TCAGCGGGGT 
CGGCTCCTGT ATGCCCCGCT GTTGTTGGTG GAAGAGGGCG CAGCGGGGGA GGGGTACCTC 
GGCAGATCCA GTGGCCCACT GCGCACCTTC ACCTGTCTGC ACGTGCCTGG AACCCACTCG 
GACCCCAGCC ATGGGAAGAG GGGCAGTGCC GACAGCTTGG TGGAGGCTGT GCTTATCTCA 
GAGGGTCTGG GCCTCTTTGC TCGAGACCCA CGTTTCGTGG CCCTGGCCAA GCAGGAGATT 
GCAGATGCGT GTCGCCTGAC GCTGGATGAG ATGGACAATG CTGCCAGTGA CCTGCTGGCA 
CAGGGAACCA GCTCTCTCTA TAGCGACGAG GAGTCCATCC TCTCCCGCTT CGATGAGGAG 
GACTTGGGAG ACGAGATGGC CTGCGTCCAC GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243719.1
CDS	32..5770
Translation	MSESEGGKGERILPSLQTLGASIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNLGSMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_001256790.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256790.3

ORF Insert Sequence:

ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC 
TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC 
AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG 
ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA 
CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG 
AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA 
GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC 
TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG 
ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT 
TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG 
CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG 
TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT 
GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC 
CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC 
GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG 
GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT 
GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT 
AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC 
AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG 
GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC 
AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA 
GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC 
AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC 
TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC 
CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA 
TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG 
GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC 
TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT 
CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT 
GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC 
AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT 
GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG 
AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA 
GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG 
GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC 
CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC 
ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT 
GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT 
TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG 
TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG 
AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA 
CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT 
GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA 
TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG 
GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT 
GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT 
GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC 
ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG 
CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC 
AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC 
ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT 
GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC 
AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG 
CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC 
ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AATGGTGGCC ACCTTGGCGA GAGCTCTGAG 
GACAGCTCCC GCATTTCCAT TACCTTCTTT CGCCTCTTCC GAGTTATGCG GCTGGTCAAG 
CTTCTCAGTA AGGGTGAAGG GATCCGCACA TTGCTCTGGA CATTCATCAA GTCCTTCCAG 
GCCTTGCCCT ATGTGGCTCT TCTCATCGCA ATGATATTCT TCATCTATGC CGTCATTGGC 
ATGCAGATGT TCGGCAAGGT GGCTCTTCAG GATGGCACAC AGATAAACCG AAACAACAAC 
TTCCAGACCT TTCCACAGGC TGTGCTGCTT CTGTTCAGGT GTGCCACTGG TGAGGCATGG 
CAGGAGATAA TGCTTGCCAG CCTTCCCGGA AATCGGTGTG ATCCTGAGTC TGACTTCGGC 
CCTGGTGAAG AGTTTACCTG TGGTAGCAAT TTTGCCATCG CCTATTTCAT CAGCTTCTTC 
ATGCTCTGTG CCTTCCTGAT CATAAATCTC TTTGTGGCTG TGATCATGGA CAACTTTGAT 
TATCTCACCA GAGATTGGTC CATCCTGGGC CCCCATCACC TTGATGAATT CAAGAGGATC 
TGGTCTGAAT ATGACCCTGG GGCCAAGGGC CGCATCAAAC ACTTGGATGT GGTTGCCCTG 
CTGAGACGTA TCCAGCCCCC TCTGGGATTT GGGAAGCTGT GCCCACACCG AGTGGCCTGC 
AAGAGACTTG TGGCAATGAA CATGCCCCTC AACTCAGATG GGACGGTGAC ATTCAACGCC 
ACACTCTTTG CCCTGGTCCG GACATCCCTG AAGATCAAAA CAGAAGGGAA CCTGGAGCAA 
GCCAACCAGG AGCTGCGGAT TGTCATCAAA AAGATCTGGA AGCGGATGAA ACAGAAGCTG 
CTAGATGAGG TCATCCCCCC ACCAGACGAG GAGGAGGTCA CCGTGGGCAA ATTCTACGCC 
ACATTTCTGA TCCAGGACTA TTTCCGCAAA TTCCGGCGGA GGAAAGAAAA AGGGCTACTA 
GGCAACGACG CCGCCCCTAG CACCTCTTCC GCCCTTCAGG CTGGTCTGCG GAGCCTGCAG 
GACTTGGGTC CTGAGATGCG GCAGGCCCTC ACCTGTGACA CAGAGGAGGA GGAAGAAGAG 
GGGCAGGAGG GAGTGGAGGA GGAAGATGAA AAGGACTTGG AAACTAACAA AGCCACGATG 
GTCTCCCAGC CCTCAGCTCG CCGGGGCTCC GGGATTTCTG TGTCTCTGCC TGTCGGGGAC 
AGACTTCCAG ATTCACTCTC CTTTGGGCCC AGTGATGATG ACAGGGGGAC TCCCACCTCC 
AGTCAGCCCA GTGTGCCCCA GGCTGGATCC AACACCCACA GGAGAGGCTC TGGGGCTCTC 
ATTTTCACCA TCCCAGAAGA AGGAAATTCT CAGCCCAAGG GAACCAAAGG GCAAAACAAG 
CAAGATGAGG ATGAGGAAGT CCCTGATCGG CTTTCCTACC TAGATGAGCA GGCAGGGACT 
CCCCCGTGCT CAGTCCTTTT GCCACCTCAC AGAGCTCAGA GATACATGGA TGGGCACCTG 
GTACCACGCC GCCGTCTGCT GCCCCCCACA CCTGCAGGTC GGAAGCCCTC CTTCACCATC 
CAGTGTCTGC AGCGCCAGGG CAGTTGTGAG GATTTACCCA TCCCAGGCAC CTATCATCGT 
GGGCGAAATT CAGGGCCCAA TAGGGCTCAG GGTTCCTGGG CAACACCACC TCAGCGGGGT 
CGGCTCCTGT ATGCCCCGCT GTTGTTGGTG GAAGAGGGCG CAGCGGGGGA GGGGTACCTC 
GGCAGATCCA GTGGCCCACT GCGCACCTTC ACCTGTCTGC ACGTGCCTGG AACCCACTCG 
GACCCCAGCC ATGGGAAGAG GGGCAGTGCC GACAGCTTGG TGGAGGCTGT GCTTATCTCA 
GAGGGTCTGG GCCTCTTTGC TCGAGACCCA CGTTTCGTGG CCCTGGCCAA GCAGGAGATT 
GCAGATGCGT GTCGCCTGAC GCTGGATGAG ATGGACAATG CTGCCAGTGA CCTGCTGGCA 
CAGGGAACCA GCTCTCTCTA TAGCGACGAG GAGTCCATCC TCTCCCGCTT CGATGAGGAG 
GACTTGGGAG ACGAGATGGC CTGCGTCCAC GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( NM_001256789.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243718.1 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA.

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CloneID	OHu21801
Clone ID Related Accession (Same CDS sequence)	NM_001256789.3 , NM_001256789.2
Accession Version	NM_001256789.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ224874.1, AC232271.2 and AF201304.1. On Jun 2, 2019 this sequence version replaced NM_001256789.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ224874.1, JF701915.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT 
GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG 
GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT 
CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG 
ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG 
GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC 
GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT 
GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA 
TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC 
GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG 
CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA 
GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG 
AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC 
CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC 
GCCGATGACA ACCTTGGGCC ACAGCTGGCC GAGCTGACCA ATAGGAGGCG TGGACGTCTG 
CGCTGGTTCA GTCATTCTAC TCGCTCCACA CACTCCACCA GCAGCCATGC CAGCCTCCCA 
GCCAGTGACA CCGGTTCCAT GACAGAGACC CAAGGCGATG AGGATGAGGA GGAGGGGGCT 
CTGGCCAGCT GTACACGCTG CCTAAACAAG ATCATGAAAA CCAGAGTCTG CCGCCGCCTC 
CGCCGAGCCA ACCGGGTCCT TCGGGCACGC TGCCGTCGGG CAGTGAAGTC CAATGCCTGC 
TACTGGGCTG TGCTGTTGCT CGTCTTCCTC AACACGTTGA CCATCGCCTC TGAGCACCAC 
GGGCAGCCTG TGTGGCTCAC CCAGATCCAG GAGTATGCCA ACAAAGTGTT GCTCTGTCTG 
TTCACGGTGG AGATGCTTCT CAAATTGTAC GGTCTGGGCC CCTCTGCCTA TGTGTCTTCC 
TTCTTCAACC GCTTTGACTG CTTTGTGGTC TGTGGGGGCA TCCTAGAGAC CACCTTGGTG 
GAGGTGGGTG CCATGCAGCC CTTGGGCATC TCAGTGCTCC GATGTGTGCG CCTCCTCAGG 
ATCTTTAAGG TCACCAGACA CTGGGCTTCT CTGAGCAATC TGGTGGCATC CCTGCTCAAT 
TCAATGAAAT CCATCGCATC CTTGCTGCTT CTCCTCTTCC TCTTCATCAT TATCTTCTCC 
CTGCTTGGCA TGCAGCTGTT TGGGGGCAAG TTCAACTTTG ACCAGACCCA CACCAAGCGA 
AGCACCTTTG ACACGTTCCC CCAGGCCCTC CTCACTGTCT TTCAGATCCT GACAGGTGAG 
GACTGGAACG TGGTCATGTA TGATGGTATC ATGGCATATG GTGGCCCCTT CTTCCCAGGA 
ATGTTGGTGT GCATCTATTT CATCATTCTC TTCATCTGTG GCAACTACAT CCTGTTGAAC 
GTGTTTCTTG CCATTGCTGT GGACAACCTG GCCAGTGGAG ATGCAGGCAC TGCCAAGGAC 
AAGGGCGGGG AGAAGAGCAA TGAGAAGGAT CTCCCACAGG AGAATGAAGG CCTGGTGCCT 
GGTGTGGAGA AAGAGGAAGA GGAGGGTGCA AGGAGGGAAG GAGCAGACAT GGAGGAGGAG 
GAGGAGGAGG AAGAAGAGGA AGAAGAGGAA GAAGAGGAAG AGGGTGCAGG GGGTGTGGAA 
CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAGGG CAGCGCCTTC 
TTCTGCCTCA GCCAAACCAA CCCGCTGAGG AAGGGCTGCC ACACCCTCAT CCACCATCAT 
GTCTTCACCA ATCTTATCCT GGTGTTCATC ATCCTCAGCA GTGTGTCCCT GGCCGCTGAG 
GACCCCATCC GAGCCCACTC CTTCCGCAAC CATATTCTGG GTTACTTCGA TTATGCCTTC 
ACCTCCATTT TCACTGTGGA GATTCTACTA AAGATGACAG TGTTTGGGGC CTTCCTGCAC 
CGCGGCTCCT TCTGCCGTAG CTGGTTTAAT ATGTTGGATC TGCTGGTGGT CAGTGTGTCC 
CTCATCTCCT TTGGCATCCA CTCCAGCGCC ATCTCGGTGG TGAAGATTCT GCGAGTACTC 
CGAGTACTGC GGCCCCTCCG AGCCATCAAC AGGGCCAAGG GACTCAAGCA TGTGGTGCAG 
TGTGTATTTG TGGCCATCCG GACCATCGGA AACATCATGA TTGTCACCAC ACTTCTGCAA 
TTTATGTTCG CCTGCATCGG GGTGCAGCTC TTCAAGGGGA AATTCTACAC CTGCACGGAC 
GAGGCCAAAC ACACCCCTCA AGAATGCAAG GGCTCCTTCC TGGTATACCC AGATGGAGAC 
GTGTCACGGC CCCTGGTCCG GGAGCGGCTC TGGGTCAACA GTGATTTCAA CTTTGACAAT 
GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCCACCT TTGAAGGCTG GCCTGCACTG 
CTATACAAGG CCATCGATGC ATATGCAGAG GACCACGGCC CCATCTATAA TTACCGTGTG 
GAGATCTCAG TGTTCTTCAT TGTCTACATC ATCATCATTG CGTTCTTCAT GATGAACATC 
TTCGTGGGCT TCGTCATCAT CACTTTCCGT GCCCAGGGCG AGCAGGAGTA CCAAAACTGT 
GAGCTGGACA AGAACCAGCG TCAATGTGTG GAATATGCCC TCAAGGCCCA GCCACTCCGC 
CGTTACATCC CCAAGAACCC GCATCAGTAT CGTGTGTGGG CCACTGTGAA CTCTGCTGCC 
TTTGAGTACC TGATGTTCCT GCTCATCCTG CTCAACACAG TTGCCCTAGC CATGCAGCAC 
TATGAGCAGA CTGCTCCCTT CAACTATGCC ATGGACATCC TCAACATGGT CTTCACTGGC 
CTCTTCACTA TTGAGATGGT GCTCAAAATC ATCGCCTTCA AGCCCAAGCA TTACTTCACT 
GATGCCTGGA ACACGTTTGA CGCTCTTATT GTGGTGGGCA GCATAGTGGA TATTGCCGTC 
ACTGAAGTCA ATAATGGTGG CCACCTTGGC GAGAGCTCTG AGGACAGCTC CCGCATTTCC 
ATTACCTTCT TTCGCCTCTT CCGAGTTATG CGGCTGGTCA AGCTTCTCAG TAAGGGTGAA 
GGGATCCGCA CATTGCTCTG GACATTCATC AAGTCCTTCC AGGCCTTGCC CTATGTGGCT 
CTTCTCATCG CAATGATATT CTTCATCTAT GCCGTCATTG GCATGCAGAT GTTCGGCAAG 
GTGGCTCTTC AGGATGGCAC ACAGATAAAC CGAAACAACA ACTTCCAGAC CTTTCCACAG 
GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAGGAGAT AATGCTTGCC 
AGCCTTCCCG GAAATCGGTG TGATCCTGAG TCTGACTTCG GCCCTGGTGA AGAGTTTACC 
TGTGGTAGCA ATTTTGCCAT CGCCTATTTC ATCAGCTTCT TCATGCTCTG TGCCTTCCTG 
ATCATAAATC TCTTTGTGGC TGTGATCATG GACAACTTTG ATTATCTCAC CAGAGATTGG 
TCCATCCTGG GCCCCCATCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCT 
GGGGCCAAGG GCCGCATCAA ACACTTGGAT GTGGTTGCCC TGCTGAGACG TATCCAGCCC 
CCTCTGGGAT TTGGGAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT TGTGGCAATG 
AACATGCCCC TCAACTCAGA TGGGACGGTG ACATTCAACG CCACACTCTT TGCCCTGGTC 
CGGACATCCC TGAAGATCAA AACAGAAGGG AACCTGGAGC AAGCCAACCA GGAGCTGCGG 
ATTGTCATCA AAAAGATCTG GAAGCGGATG AAACAGAAGC TGCTAGATGA GGTCATCCCC 
CCACCAGACG AGGAGGAGGT CACCGTGGGC AAATTCTACG CCACATTTCT GATCCAGGAC 
TATTTCCGCA AATTCCGGCG GAGGAAAGAA AAAGGGCTAC TAGGCAACGA CGCCGCCCCT 
AGCACCTCTT CCGCCCTTCA GGCTGGTCTG CGGAGCCTGC AGGACTTGGG TCCTGAGATG 
CGGCAGGCCC TCACCTGTGA CACAGAGGAG GAGGAAGAAG AGGGGCAGGA GGGAGTGGAG 
GAGGAAGATG AAAAGGACTT GGAAACTAAC AAAGCCACGA TGGTCTCCCA GCCCTCAGCT 
CGCCGGGGCT CCGGGATTTC TGTGTCTCTG CCTGTCGGGG ACAGACTTCC AGATTCACTC 
TCCTTTGGGC CCAGTGATGA TGACAGGGGG ACTCCCACCT CCAGTCAGCC CAGTGTGCCC 
CAGGCTGGAT CCAACACCCA CAGGAGAGGC TCTGGGGCTC TCATTTTCAC CATCCCAGAA 
GAAGGAAATT CTCAGCCCAA GGGAACCAAA GGGCAAAACA AGCAAGATGA GGATGAGGAA 
GTCCCTGATC GGCTTTCCTA CCTAGATGAG CAGGCAGGGA CTCCCCCGTG CTCAGTCCTT 
TTGCCACCTC ACAGAGCTCA GAGATACATG GATGGGCACC TGGTACCACG CCGCCGTCTG 
CTGCCCCCCA CACCTGCAGG TCGGAAGCCC TCCTTCACCA TCCAGTGTCT GCAGCGCCAG 
GGCAGTTGTG AGGATTTACC CATCCCAGGC ACCTATCATC GTGGGCGAAA TTCAGGGCCC 
AATAGGGCTC AGGGTTCCTG GGCAACACCA CCTCAGCGGG GTCGGCTCCT GTATGCCCCG 
CTGTTGTTGG TGGAAGAGGG CGCAGCGGGG GAGGGGTACC TCGGCAGATC CAGTGGCCCA 
CTGCGCACCT TCACCTGTCT GCACGTGCCT GGAACCCACT CGGACCCCAG CCATGGGAAG 
AGGGGCAGTG CCGACAGCTT GGTGGAGGCT GTGCTTATCT CAGAGGGTCT GGGCCTCTTT 
GCTCGAGACC CACGTTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC GTGTCGCCTG 
ACGCTGGATG AGATGGACAA TGCTGCCAGT GACCTGCTGG CACAGGGAAC CAGCTCTCTC 
TATAGCGACG AGGAGTCCAT CCTCTCCCGC TTCGATGAGG AGGACTTGGG AGACGAGATG 
GCCTGCGTCC ACGCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243718.1
CDS	32..5932
Translation	MSESEGGKDTTPEPSPANGAGPGPEWGLCPGPPAVEGESSGASGLGTPKRRNQHSKHKTVAVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNLGPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_001256789.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256789.3

ORF Insert Sequence:

ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT 
GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG 
GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT 
CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG 
ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG 
GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC 
GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT 
GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA 
TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC 
GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG 
CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA 
GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG 
AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC 
CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC 
GCCGATGACA ACCTTGGGCC ACAGCTGGCC GAGCTGACCA ATAGGAGGCG TGGACGTCTG 
CGCTGGTTCA GTCATTCTAC TCGCTCCACA CACTCCACCA GCAGCCATGC CAGCCTCCCA 
GCCAGTGACA CCGGTTCCAT GACAGAGACC CAAGGCGATG AGGATGAGGA GGAGGGGGCT 
CTGGCCAGCT GTACACGCTG CCTAAACAAG ATCATGAAAA CCAGAGTCTG CCGCCGCCTC 
CGCCGAGCCA ACCGGGTCCT TCGGGCACGC TGCCGTCGGG CAGTGAAGTC CAATGCCTGC 
TACTGGGCTG TGCTGTTGCT CGTCTTCCTC AACACGTTGA CCATCGCCTC TGAGCACCAC 
GGGCAGCCTG TGTGGCTCAC CCAGATCCAG GAGTATGCCA ACAAAGTGTT GCTCTGTCTG 
TTCACGGTGG AGATGCTTCT CAAATTGTAC GGTCTGGGCC CCTCTGCCTA TGTGTCTTCC 
TTCTTCAACC GCTTTGACTG CTTTGTGGTC TGTGGGGGCA TCCTAGAGAC CACCTTGGTG 
GAGGTGGGTG CCATGCAGCC CTTGGGCATC TCAGTGCTCC GATGTGTGCG CCTCCTCAGG 
ATCTTTAAGG TCACCAGACA CTGGGCTTCT CTGAGCAATC TGGTGGCATC CCTGCTCAAT 
TCAATGAAAT CCATCGCATC CTTGCTGCTT CTCCTCTTCC TCTTCATCAT TATCTTCTCC 
CTGCTTGGCA TGCAGCTGTT TGGGGGCAAG TTCAACTTTG ACCAGACCCA CACCAAGCGA 
AGCACCTTTG ACACGTTCCC CCAGGCCCTC CTCACTGTCT TTCAGATCCT GACAGGTGAG 
GACTGGAACG TGGTCATGTA TGATGGTATC ATGGCATATG GTGGCCCCTT CTTCCCAGGA 
ATGTTGGTGT GCATCTATTT CATCATTCTC TTCATCTGTG GCAACTACAT CCTGTTGAAC 
GTGTTTCTTG CCATTGCTGT GGACAACCTG GCCAGTGGAG ATGCAGGCAC TGCCAAGGAC 
AAGGGCGGGG AGAAGAGCAA TGAGAAGGAT CTCCCACAGG AGAATGAAGG CCTGGTGCCT 
GGTGTGGAGA AAGAGGAAGA GGAGGGTGCA AGGAGGGAAG GAGCAGACAT GGAGGAGGAG 
GAGGAGGAGG AAGAAGAGGA AGAAGAGGAA GAAGAGGAAG AGGGTGCAGG GGGTGTGGAA 
CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAGGG CAGCGCCTTC 
TTCTGCCTCA GCCAAACCAA CCCGCTGAGG AAGGGCTGCC ACACCCTCAT CCACCATCAT 
GTCTTCACCA ATCTTATCCT GGTGTTCATC ATCCTCAGCA GTGTGTCCCT GGCCGCTGAG 
GACCCCATCC GAGCCCACTC CTTCCGCAAC CATATTCTGG GTTACTTCGA TTATGCCTTC 
ACCTCCATTT TCACTGTGGA GATTCTACTA AAGATGACAG TGTTTGGGGC CTTCCTGCAC 
CGCGGCTCCT TCTGCCGTAG CTGGTTTAAT ATGTTGGATC TGCTGGTGGT CAGTGTGTCC 
CTCATCTCCT TTGGCATCCA CTCCAGCGCC ATCTCGGTGG TGAAGATTCT GCGAGTACTC 
CGAGTACTGC GGCCCCTCCG AGCCATCAAC AGGGCCAAGG GACTCAAGCA TGTGGTGCAG 
TGTGTATTTG TGGCCATCCG GACCATCGGA AACATCATGA TTGTCACCAC ACTTCTGCAA 
TTTATGTTCG CCTGCATCGG GGTGCAGCTC TTCAAGGGGA AATTCTACAC CTGCACGGAC 
GAGGCCAAAC ACACCCCTCA AGAATGCAAG GGCTCCTTCC TGGTATACCC AGATGGAGAC 
GTGTCACGGC CCCTGGTCCG GGAGCGGCTC TGGGTCAACA GTGATTTCAA CTTTGACAAT 
GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCCACCT TTGAAGGCTG GCCTGCACTG 
CTATACAAGG CCATCGATGC ATATGCAGAG GACCACGGCC CCATCTATAA TTACCGTGTG 
GAGATCTCAG TGTTCTTCAT TGTCTACATC ATCATCATTG CGTTCTTCAT GATGAACATC 
TTCGTGGGCT TCGTCATCAT CACTTTCCGT GCCCAGGGCG AGCAGGAGTA CCAAAACTGT 
GAGCTGGACA AGAACCAGCG TCAATGTGTG GAATATGCCC TCAAGGCCCA GCCACTCCGC 
CGTTACATCC CCAAGAACCC GCATCAGTAT CGTGTGTGGG CCACTGTGAA CTCTGCTGCC 
TTTGAGTACC TGATGTTCCT GCTCATCCTG CTCAACACAG TTGCCCTAGC CATGCAGCAC 
TATGAGCAGA CTGCTCCCTT CAACTATGCC ATGGACATCC TCAACATGGT CTTCACTGGC 
CTCTTCACTA TTGAGATGGT GCTCAAAATC ATCGCCTTCA AGCCCAAGCA TTACTTCACT 
GATGCCTGGA ACACGTTTGA CGCTCTTATT GTGGTGGGCA GCATAGTGGA TATTGCCGTC 
ACTGAAGTCA ATAATGGTGG CCACCTTGGC GAGAGCTCTG AGGACAGCTC CCGCATTTCC 
ATTACCTTCT TTCGCCTCTT CCGAGTTATG CGGCTGGTCA AGCTTCTCAG TAAGGGTGAA 
GGGATCCGCA CATTGCTCTG GACATTCATC AAGTCCTTCC AGGCCTTGCC CTATGTGGCT 
CTTCTCATCG CAATGATATT CTTCATCTAT GCCGTCATTG GCATGCAGAT GTTCGGCAAG 
GTGGCTCTTC AGGATGGCAC ACAGATAAAC CGAAACAACA ACTTCCAGAC CTTTCCACAG 
GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAGGAGAT AATGCTTGCC 
AGCCTTCCCG GAAATCGGTG TGATCCTGAG TCTGACTTCG GCCCTGGTGA AGAGTTTACC 
TGTGGTAGCA ATTTTGCCAT CGCCTATTTC ATCAGCTTCT TCATGCTCTG TGCCTTCCTG 
ATCATAAATC TCTTTGTGGC TGTGATCATG GACAACTTTG ATTATCTCAC CAGAGATTGG 
TCCATCCTGG GCCCCCATCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCT 
GGGGCCAAGG GCCGCATCAA ACACTTGGAT GTGGTTGCCC TGCTGAGACG TATCCAGCCC 
CCTCTGGGAT TTGGGAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT TGTGGCAATG 
AACATGCCCC TCAACTCAGA TGGGACGGTG ACATTCAACG CCACACTCTT TGCCCTGGTC 
CGGACATCCC TGAAGATCAA AACAGAAGGG AACCTGGAGC AAGCCAACCA GGAGCTGCGG 
ATTGTCATCA AAAAGATCTG GAAGCGGATG AAACAGAAGC TGCTAGATGA GGTCATCCCC 
CCACCAGACG AGGAGGAGGT CACCGTGGGC AAATTCTACG CCACATTTCT GATCCAGGAC 
TATTTCCGCA AATTCCGGCG GAGGAAAGAA AAAGGGCTAC TAGGCAACGA CGCCGCCCCT 
AGCACCTCTT CCGCCCTTCA GGCTGGTCTG CGGAGCCTGC AGGACTTGGG TCCTGAGATG 
CGGCAGGCCC TCACCTGTGA CACAGAGGAG GAGGAAGAAG AGGGGCAGGA GGGAGTGGAG 
GAGGAAGATG AAAAGGACTT GGAAACTAAC AAAGCCACGA TGGTCTCCCA GCCCTCAGCT 
CGCCGGGGCT CCGGGATTTC TGTGTCTCTG CCTGTCGGGG ACAGACTTCC AGATTCACTC 
TCCTTTGGGC CCAGTGATGA TGACAGGGGG ACTCCCACCT CCAGTCAGCC CAGTGTGCCC 
CAGGCTGGAT CCAACACCCA CAGGAGAGGC TCTGGGGCTC TCATTTTCAC CATCCCAGAA 
GAAGGAAATT CTCAGCCCAA GGGAACCAAA GGGCAAAACA AGCAAGATGA GGATGAGGAA 
GTCCCTGATC GGCTTTCCTA CCTAGATGAG CAGGCAGGGA CTCCCCCGTG CTCAGTCCTT 
TTGCCACCTC ACAGAGCTCA GAGATACATG GATGGGCACC TGGTACCACG CCGCCGTCTG 
CTGCCCCCCA CACCTGCAGG TCGGAAGCCC TCCTTCACCA TCCAGTGTCT GCAGCGCCAG 
GGCAGTTGTG AGGATTTACC CATCCCAGGC ACCTATCATC GTGGGCGAAA TTCAGGGCCC 
AATAGGGCTC AGGGTTCCTG GGCAACACCA CCTCAGCGGG GTCGGCTCCT GTATGCCCCG 
CTGTTGTTGG TGGAAGAGGG CGCAGCGGGG GAGGGGTACC TCGGCAGATC CAGTGGCCCA 
CTGCGCACCT TCACCTGTCT GCACGTGCCT GGAACCCACT CGGACCCCAG CCATGGGAAG 
AGGGGCAGTG CCGACAGCTT GGTGGAGGCT GTGCTTATCT CAGAGGGTCT GGGCCTCTTT 
GCTCGAGACC CACGTTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC GTGTCGCCTG 
ACGCTGGATG AGATGGACAA TGCTGCCAGT GACCTGCTGG CACAGGGAAC CAGCTCTCTC 
TATAGCGACG AGGAGTCCAT CCTCTCCCGC TTCGATGAGG AGGACTTGGG AGACGAGATG 
GCCTGCGTCC ACGCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( NM_001256789.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243718.1 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA.

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CloneID	OHu21801
Clone ID Related Accession (Same CDS sequence)	NM_001256789.3 , NM_001256789.2
Accession Version	NM_001256789.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ224874.1, AC232271.2 and AF201304.1. On Aug 21, 2013 this sequence version replaced NM_001256789.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ224874.1, JF701915.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT 
GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG 
GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT 
CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG 
ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG 
GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC 
GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT 
GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA 
TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC 
GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG 
CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA 
GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG 
AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC 
CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC 
GCCGATGACA ACCTTGGGCC ACAGCTGGCC GAGCTGACCA ATAGGAGGCG TGGACGTCTG 
CGCTGGTTCA GTCATTCTAC TCGCTCCACA CACTCCACCA GCAGCCATGC CAGCCTCCCA 
GCCAGTGACA CCGGTTCCAT GACAGAGACC CAAGGCGATG AGGATGAGGA GGAGGGGGCT 
CTGGCCAGCT GTACACGCTG CCTAAACAAG ATCATGAAAA CCAGAGTCTG CCGCCGCCTC 
CGCCGAGCCA ACCGGGTCCT TCGGGCACGC TGCCGTCGGG CAGTGAAGTC CAATGCCTGC 
TACTGGGCTG TGCTGTTGCT CGTCTTCCTC AACACGTTGA CCATCGCCTC TGAGCACCAC 
GGGCAGCCTG TGTGGCTCAC CCAGATCCAG GAGTATGCCA ACAAAGTGTT GCTCTGTCTG 
TTCACGGTGG AGATGCTTCT CAAATTGTAC GGTCTGGGCC CCTCTGCCTA TGTGTCTTCC 
TTCTTCAACC GCTTTGACTG CTTTGTGGTC TGTGGGGGCA TCCTAGAGAC CACCTTGGTG 
GAGGTGGGTG CCATGCAGCC CTTGGGCATC TCAGTGCTCC GATGTGTGCG CCTCCTCAGG 
ATCTTTAAGG TCACCAGACA CTGGGCTTCT CTGAGCAATC TGGTGGCATC CCTGCTCAAT 
TCAATGAAAT CCATCGCATC CTTGCTGCTT CTCCTCTTCC TCTTCATCAT TATCTTCTCC 
CTGCTTGGCA TGCAGCTGTT TGGGGGCAAG TTCAACTTTG ACCAGACCCA CACCAAGCGA 
AGCACCTTTG ACACGTTCCC CCAGGCCCTC CTCACTGTCT TTCAGATCCT GACAGGTGAG 
GACTGGAACG TGGTCATGTA TGATGGTATC ATGGCATATG GTGGCCCCTT CTTCCCAGGA 
ATGTTGGTGT GCATCTATTT CATCATTCTC TTCATCTGTG GCAACTACAT CCTGTTGAAC 
GTGTTTCTTG CCATTGCTGT GGACAACCTG GCCAGTGGAG ATGCAGGCAC TGCCAAGGAC 
AAGGGCGGGG AGAAGAGCAA TGAGAAGGAT CTCCCACAGG AGAATGAAGG CCTGGTGCCT 
GGTGTGGAGA AAGAGGAAGA GGAGGGTGCA AGGAGGGAAG GAGCAGACAT GGAGGAGGAG 
GAGGAGGAGG AAGAAGAGGA AGAAGAGGAA GAAGAGGAAG AGGGTGCAGG GGGTGTGGAA 
CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAGGG CAGCGCCTTC 
TTCTGCCTCA GCCAAACCAA CCCGCTGAGG AAGGGCTGCC ACACCCTCAT CCACCATCAT 
GTCTTCACCA ATCTTATCCT GGTGTTCATC ATCCTCAGCA GTGTGTCCCT GGCCGCTGAG 
GACCCCATCC GAGCCCACTC CTTCCGCAAC CATATTCTGG GTTACTTCGA TTATGCCTTC 
ACCTCCATTT TCACTGTGGA GATTCTACTA AAGATGACAG TGTTTGGGGC CTTCCTGCAC 
CGCGGCTCCT TCTGCCGTAG CTGGTTTAAT ATGTTGGATC TGCTGGTGGT CAGTGTGTCC 
CTCATCTCCT TTGGCATCCA CTCCAGCGCC ATCTCGGTGG TGAAGATTCT GCGAGTACTC 
CGAGTACTGC GGCCCCTCCG AGCCATCAAC AGGGCCAAGG GACTCAAGCA TGTGGTGCAG 
TGTGTATTTG TGGCCATCCG GACCATCGGA AACATCATGA TTGTCACCAC ACTTCTGCAA 
TTTATGTTCG CCTGCATCGG GGTGCAGCTC TTCAAGGGGA AATTCTACAC CTGCACGGAC 
GAGGCCAAAC ACACCCCTCA AGAATGCAAG GGCTCCTTCC TGGTATACCC AGATGGAGAC 
GTGTCACGGC CCCTGGTCCG GGAGCGGCTC TGGGTCAACA GTGATTTCAA CTTTGACAAT 
GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCCACCT TTGAAGGCTG GCCTGCACTG 
CTATACAAGG CCATCGATGC ATATGCAGAG GACCACGGCC CCATCTATAA TTACCGTGTG 
GAGATCTCAG TGTTCTTCAT TGTCTACATC ATCATCATTG CGTTCTTCAT GATGAACATC 
TTCGTGGGCT TCGTCATCAT CACTTTCCGT GCCCAGGGCG AGCAGGAGTA CCAAAACTGT 
GAGCTGGACA AGAACCAGCG TCAATGTGTG GAATATGCCC TCAAGGCCCA GCCACTCCGC 
CGTTACATCC CCAAGAACCC GCATCAGTAT CGTGTGTGGG CCACTGTGAA CTCTGCTGCC 
TTTGAGTACC TGATGTTCCT GCTCATCCTG CTCAACACAG TTGCCCTAGC CATGCAGCAC 
TATGAGCAGA CTGCTCCCTT CAACTATGCC ATGGACATCC TCAACATGGT CTTCACTGGC 
CTCTTCACTA TTGAGATGGT GCTCAAAATC ATCGCCTTCA AGCCCAAGCA TTACTTCACT 
GATGCCTGGA ACACGTTTGA CGCTCTTATT GTGGTGGGCA GCATAGTGGA TATTGCCGTC 
ACTGAAGTCA ATAATGGTGG CCACCTTGGC GAGAGCTCTG AGGACAGCTC CCGCATTTCC 
ATTACCTTCT TTCGCCTCTT CCGAGTTATG CGGCTGGTCA AGCTTCTCAG TAAGGGTGAA 
GGGATCCGCA CATTGCTCTG GACATTCATC AAGTCCTTCC AGGCCTTGCC CTATGTGGCT 
CTTCTCATCG CAATGATATT CTTCATCTAT GCCGTCATTG GCATGCAGAT GTTCGGCAAG 
GTGGCTCTTC AGGATGGCAC ACAGATAAAC CGAAACAACA ACTTCCAGAC CTTTCCACAG 
GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAGGAGAT AATGCTTGCC 
AGCCTTCCCG GAAATCGGTG TGATCCTGAG TCTGACTTCG GCCCTGGTGA AGAGTTTACC 
TGTGGTAGCA ATTTTGCCAT CGCCTATTTC ATCAGCTTCT TCATGCTCTG TGCCTTCCTG 
ATCATAAATC TCTTTGTGGC TGTGATCATG GACAACTTTG ATTATCTCAC CAGAGATTGG 
TCCATCCTGG GCCCCCATCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCT 
GGGGCCAAGG GCCGCATCAA ACACTTGGAT GTGGTTGCCC TGCTGAGACG TATCCAGCCC 
CCTCTGGGAT TTGGGAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT TGTGGCAATG 
AACATGCCCC TCAACTCAGA TGGGACGGTG ACATTCAACG CCACACTCTT TGCCCTGGTC 
CGGACATCCC TGAAGATCAA AACAGAAGGG AACCTGGAGC AAGCCAACCA GGAGCTGCGG 
ATTGTCATCA AAAAGATCTG GAAGCGGATG AAACAGAAGC TGCTAGATGA GGTCATCCCC 
CCACCAGACG AGGAGGAGGT CACCGTGGGC AAATTCTACG CCACATTTCT GATCCAGGAC 
TATTTCCGCA AATTCCGGCG GAGGAAAGAA AAAGGGCTAC TAGGCAACGA CGCCGCCCCT 
AGCACCTCTT CCGCCCTTCA GGCTGGTCTG CGGAGCCTGC AGGACTTGGG TCCTGAGATG 
CGGCAGGCCC TCACCTGTGA CACAGAGGAG GAGGAAGAAG AGGGGCAGGA GGGAGTGGAG 
GAGGAAGATG AAAAGGACTT GGAAACTAAC AAAGCCACGA TGGTCTCCCA GCCCTCAGCT 
CGCCGGGGCT CCGGGATTTC TGTGTCTCTG CCTGTCGGGG ACAGACTTCC AGATTCACTC 
TCCTTTGGGC CCAGTGATGA TGACAGGGGG ACTCCCACCT CCAGTCAGCC CAGTGTGCCC 
CAGGCTGGAT CCAACACCCA CAGGAGAGGC TCTGGGGCTC TCATTTTCAC CATCCCAGAA 
GAAGGAAATT CTCAGCCCAA GGGAACCAAA GGGCAAAACA AGCAAGATGA GGATGAGGAA 
GTCCCTGATC GGCTTTCCTA CCTAGATGAG CAGGCAGGGA CTCCCCCGTG CTCAGTCCTT 
TTGCCACCTC ACAGAGCTCA GAGATACATG GATGGGCACC TGGTACCACG CCGCCGTCTG 
CTGCCCCCCA CACCTGCAGG TCGGAAGCCC TCCTTCACCA TCCAGTGTCT GCAGCGCCAG 
GGCAGTTGTG AGGATTTACC CATCCCAGGC ACCTATCATC GTGGGCGAAA TTCAGGGCCC 
AATAGGGCTC AGGGTTCCTG GGCAACACCA CCTCAGCGGG GTCGGCTCCT GTATGCCCCG 
CTGTTGTTGG TGGAAGAGGG CGCAGCGGGG GAGGGGTACC TCGGCAGATC CAGTGGCCCA 
CTGCGCACCT TCACCTGTCT GCACGTGCCT GGAACCCACT CGGACCCCAG CCATGGGAAG 
AGGGGCAGTG CCGACAGCTT GGTGGAGGCT GTGCTTATCT CAGAGGGTCT GGGCCTCTTT 
GCTCGAGACC CACGTTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC GTGTCGCCTG 
ACGCTGGATG AGATGGACAA TGCTGCCAGT GACCTGCTGG CACAGGGAAC CAGCTCTCTC 
TATAGCGACG AGGAGTCCAT CCTCTCCCGC TTCGATGAGG AGGACTTGGG AGACGAGATG 
GCCTGCGTCC ACGCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243718.1
CDS	63..5963
Translation	MSESEGGKDTTPEPSPANGAGPGPEWGLCPGPPAVEGESSGASGLGTPKRRNQHSKHKTVAVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNLGPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_001256789.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256789.2

ORF Insert Sequence:

ATGTCGGAAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGGCA 
GGCCCTGGTC CCGAATGGGG GCTGTGCCCC GGGCCCCCAG CTGTGGAAGG TGAAAGCAGT 
GGGGCATCAG GCCTAGGGAC CCCTAAGCGA AGAAACCAGC ACAGCAAGCA CAAGACAGTG 
GCAGTGGCCA GTGCCCAGCG GTCACCTCGG GCACTCTTCT GCCTCACCCT GGCCAATCCT 
CTGCGACGGT CCTGCATCAG CATCGTGGAG TGGAAGCCCT TCGACATCCT CATCCTGCTG 
ACCATCTTTG CCAACTGCGT GGCCCTGGGA GTTTACATCC CCTTCCCTGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAGGTG GAGTACGTAT TCCTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AATGGCTGGA ACCTACTCGA CTTCATCATC GTCGTGGTCG GGCTGTTCAG CGTTCTGCTG 
GAGCAGGGCC CCGGACGGCC AGGCGACGCC CCGCACACCG GGGGAAAGCC AGGAGGCTTC 
GATGTGAAGG CATTGAGGGC GTTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCGAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GCTGCACATT 
GCACTGCTCG TGCTCTTCGT CATCATCATT TATGCCATCA TTGGGCTCGA GCTGTTCCTT 
GGACGAATGC ACAAGACGTG CTACTTCCTG GGATCCGACA TGGAAGCGGA GGAGGACCCA 
TCGCCCTGTG CGTCTTCGGG ATCAGGGCGT GCGTGCACGC TGAACCAGAC TGAGTGCCGC 
GGGCGCTGGC CAGGGCCCAA TGGAGGCATC ACCAACTTTG ACAACTTCTT CTTCGCCATG 
CTGACAGTCT TCCAGTGTGT CACCATGGAA GGCTGGACCG ATGTGCTCTA CTGGATGCAA 
GATGCCATGG GGTATGAACT GCCCTGGGTG TACTTTGTGA GCCTTGTCAT CTTTGGGTCC 
TTCTTCGTCC TCAACCTTGT GCTTGGCGTC CTGAGTGGGG AGTTCTCCAA GGAGAGAGAG 
AAAGCGAAAG CTCGCGGGGA CTTCCAGAAG CAGCGGGAGA AGCAGCAGAT GGAGGAAGAC 
CTGCGGGGCT ACCTGGACTG GATCACTCAA GCCGAAGAGC TGGACATGGA GGACCCCTCC 
GCCGATGACA ACCTTGGGCC ACAGCTGGCC GAGCTGACCA ATAGGAGGCG TGGACGTCTG 
CGCTGGTTCA GTCATTCTAC TCGCTCCACA CACTCCACCA GCAGCCATGC CAGCCTCCCA 
GCCAGTGACA CCGGTTCCAT GACAGAGACC CAAGGCGATG AGGATGAGGA GGAGGGGGCT 
CTGGCCAGCT GTACACGCTG CCTAAACAAG ATCATGAAAA CCAGAGTCTG CCGCCGCCTC 
CGCCGAGCCA ACCGGGTCCT TCGGGCACGC TGCCGTCGGG CAGTGAAGTC CAATGCCTGC 
TACTGGGCTG TGCTGTTGCT CGTCTTCCTC AACACGTTGA CCATCGCCTC TGAGCACCAC 
GGGCAGCCTG TGTGGCTCAC CCAGATCCAG GAGTATGCCA ACAAAGTGTT GCTCTGTCTG 
TTCACGGTGG AGATGCTTCT CAAATTGTAC GGTCTGGGCC CCTCTGCCTA TGTGTCTTCC 
TTCTTCAACC GCTTTGACTG CTTTGTGGTC TGTGGGGGCA TCCTAGAGAC CACCTTGGTG 
GAGGTGGGTG CCATGCAGCC CTTGGGCATC TCAGTGCTCC GATGTGTGCG CCTCCTCAGG 
ATCTTTAAGG TCACCAGACA CTGGGCTTCT CTGAGCAATC TGGTGGCATC CCTGCTCAAT 
TCAATGAAAT CCATCGCATC CTTGCTGCTT CTCCTCTTCC TCTTCATCAT TATCTTCTCC 
CTGCTTGGCA TGCAGCTGTT TGGGGGCAAG TTCAACTTTG ACCAGACCCA CACCAAGCGA 
AGCACCTTTG ACACGTTCCC CCAGGCCCTC CTCACTGTCT TTCAGATCCT GACAGGTGAG 
GACTGGAACG TGGTCATGTA TGATGGTATC ATGGCATATG GTGGCCCCTT CTTCCCAGGA 
ATGTTGGTGT GCATCTATTT CATCATTCTC TTCATCTGTG GCAACTACAT CCTGTTGAAC 
GTGTTTCTTG CCATTGCTGT GGACAACCTG GCCAGTGGAG ATGCAGGCAC TGCCAAGGAC 
AAGGGCGGGG AGAAGAGCAA TGAGAAGGAT CTCCCACAGG AGAATGAAGG CCTGGTGCCT 
GGTGTGGAGA AAGAGGAAGA GGAGGGTGCA AGGAGGGAAG GAGCAGACAT GGAGGAGGAG 
GAGGAGGAGG AAGAAGAGGA AGAAGAGGAA GAAGAGGAAG AGGGTGCAGG GGGTGTGGAA 
CTCCTGCAGG AAGTTGTACC CAAGGAGAAG GTGGTACCCA TCCCTGAGGG CAGCGCCTTC 
TTCTGCCTCA GCCAAACCAA CCCGCTGAGG AAGGGCTGCC ACACCCTCAT CCACCATCAT 
GTCTTCACCA ATCTTATCCT GGTGTTCATC ATCCTCAGCA GTGTGTCCCT GGCCGCTGAG 
GACCCCATCC GAGCCCACTC CTTCCGCAAC CATATTCTGG GTTACTTCGA TTATGCCTTC 
ACCTCCATTT TCACTGTGGA GATTCTACTA AAGATGACAG TGTTTGGGGC CTTCCTGCAC 
CGCGGCTCCT TCTGCCGTAG CTGGTTTAAT ATGTTGGATC TGCTGGTGGT CAGTGTGTCC 
CTCATCTCCT TTGGCATCCA CTCCAGCGCC ATCTCGGTGG TGAAGATTCT GCGAGTACTC 
CGAGTACTGC GGCCCCTCCG AGCCATCAAC AGGGCCAAGG GACTCAAGCA TGTGGTGCAG 
TGTGTATTTG TGGCCATCCG GACCATCGGA AACATCATGA TTGTCACCAC ACTTCTGCAA 
TTTATGTTCG CCTGCATCGG GGTGCAGCTC TTCAAGGGGA AATTCTACAC CTGCACGGAC 
GAGGCCAAAC ACACCCCTCA AGAATGCAAG GGCTCCTTCC TGGTATACCC AGATGGAGAC 
GTGTCACGGC CCCTGGTCCG GGAGCGGCTC TGGGTCAACA GTGATTTCAA CTTTGACAAT 
GTCCTTTCAG CCATGATGGC CCTGTTCACT GTCTCCACCT TTGAAGGCTG GCCTGCACTG 
CTATACAAGG CCATCGATGC ATATGCAGAG GACCACGGCC CCATCTATAA TTACCGTGTG 
GAGATCTCAG TGTTCTTCAT TGTCTACATC ATCATCATTG CGTTCTTCAT GATGAACATC 
TTCGTGGGCT TCGTCATCAT CACTTTCCGT GCCCAGGGCG AGCAGGAGTA CCAAAACTGT 
GAGCTGGACA AGAACCAGCG TCAATGTGTG GAATATGCCC TCAAGGCCCA GCCACTCCGC 
CGTTACATCC CCAAGAACCC GCATCAGTAT CGTGTGTGGG CCACTGTGAA CTCTGCTGCC 
TTTGAGTACC TGATGTTCCT GCTCATCCTG CTCAACACAG TTGCCCTAGC CATGCAGCAC 
TATGAGCAGA CTGCTCCCTT CAACTATGCC ATGGACATCC TCAACATGGT CTTCACTGGC 
CTCTTCACTA TTGAGATGGT GCTCAAAATC ATCGCCTTCA AGCCCAAGCA TTACTTCACT 
GATGCCTGGA ACACGTTTGA CGCTCTTATT GTGGTGGGCA GCATAGTGGA TATTGCCGTC 
ACTGAAGTCA ATAATGGTGG CCACCTTGGC GAGAGCTCTG AGGACAGCTC CCGCATTTCC 
ATTACCTTCT TTCGCCTCTT CCGAGTTATG CGGCTGGTCA AGCTTCTCAG TAAGGGTGAA 
GGGATCCGCA CATTGCTCTG GACATTCATC AAGTCCTTCC AGGCCTTGCC CTATGTGGCT 
CTTCTCATCG CAATGATATT CTTCATCTAT GCCGTCATTG GCATGCAGAT GTTCGGCAAG 
GTGGCTCTTC AGGATGGCAC ACAGATAAAC CGAAACAACA ACTTCCAGAC CTTTCCACAG 
GCTGTGCTGC TTCTGTTCAG GTGTGCCACT GGTGAGGCAT GGCAGGAGAT AATGCTTGCC 
AGCCTTCCCG GAAATCGGTG TGATCCTGAG TCTGACTTCG GCCCTGGTGA AGAGTTTACC 
TGTGGTAGCA ATTTTGCCAT CGCCTATTTC ATCAGCTTCT TCATGCTCTG TGCCTTCCTG 
ATCATAAATC TCTTTGTGGC TGTGATCATG GACAACTTTG ATTATCTCAC CAGAGATTGG 
TCCATCCTGG GCCCCCATCA CCTTGATGAA TTCAAGAGGA TCTGGTCTGA ATATGACCCT 
GGGGCCAAGG GCCGCATCAA ACACTTGGAT GTGGTTGCCC TGCTGAGACG TATCCAGCCC 
CCTCTGGGAT TTGGGAAGCT GTGCCCACAC CGAGTGGCCT GCAAGAGACT TGTGGCAATG 
AACATGCCCC TCAACTCAGA TGGGACGGTG ACATTCAACG CCACACTCTT TGCCCTGGTC 
CGGACATCCC TGAAGATCAA AACAGAAGGG AACCTGGAGC AAGCCAACCA GGAGCTGCGG 
ATTGTCATCA AAAAGATCTG GAAGCGGATG AAACAGAAGC TGCTAGATGA GGTCATCCCC 
CCACCAGACG AGGAGGAGGT CACCGTGGGC AAATTCTACG CCACATTTCT GATCCAGGAC 
TATTTCCGCA AATTCCGGCG GAGGAAAGAA AAAGGGCTAC TAGGCAACGA CGCCGCCCCT 
AGCACCTCTT CCGCCCTTCA GGCTGGTCTG CGGAGCCTGC AGGACTTGGG TCCTGAGATG 
CGGCAGGCCC TCACCTGTGA CACAGAGGAG GAGGAAGAAG AGGGGCAGGA GGGAGTGGAG 
GAGGAAGATG AAAAGGACTT GGAAACTAAC AAAGCCACGA TGGTCTCCCA GCCCTCAGCT 
CGCCGGGGCT CCGGGATTTC TGTGTCTCTG CCTGTCGGGG ACAGACTTCC AGATTCACTC 
TCCTTTGGGC CCAGTGATGA TGACAGGGGG ACTCCCACCT CCAGTCAGCC CAGTGTGCCC 
CAGGCTGGAT CCAACACCCA CAGGAGAGGC TCTGGGGCTC TCATTTTCAC CATCCCAGAA 
GAAGGAAATT CTCAGCCCAA GGGAACCAAA GGGCAAAACA AGCAAGATGA GGATGAGGAA 
GTCCCTGATC GGCTTTCCTA CCTAGATGAG CAGGCAGGGA CTCCCCCGTG CTCAGTCCTT 
TTGCCACCTC ACAGAGCTCA GAGATACATG GATGGGCACC TGGTACCACG CCGCCGTCTG 
CTGCCCCCCA CACCTGCAGG TCGGAAGCCC TCCTTCACCA TCCAGTGTCT GCAGCGCCAG 
GGCAGTTGTG AGGATTTACC CATCCCAGGC ACCTATCATC GTGGGCGAAA TTCAGGGCCC 
AATAGGGCTC AGGGTTCCTG GGCAACACCA CCTCAGCGGG GTCGGCTCCT GTATGCCCCG 
CTGTTGTTGG TGGAAGAGGG CGCAGCGGGG GAGGGGTACC TCGGCAGATC CAGTGGCCCA 
CTGCGCACCT TCACCTGTCT GCACGTGCCT GGAACCCACT CGGACCCCAG CCATGGGAAG 
AGGGGCAGTG CCGACAGCTT GGTGGAGGCT GTGCTTATCT CAGAGGGTCT GGGCCTCTTT 
GCTCGAGACC CACGTTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC GTGTCGCCTG 
ACGCTGGATG AGATGGACAA TGCTGCCAGT GACCTGCTGG CACAGGGAAC CAGCTCTCTC 
TATAGCGACG AGGAGTCCAT CCTCTCCCGC TTCGATGAGG AGGACTTGGG AGACGAGATG 
GCCTGCGTCC ACGCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( XM_011543983.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542285.1 Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X2, mRNA.

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CloneID	OHu56583
Clone ID Related Accession (Same CDS sequence)	XM_011543983.2
Accession Version	XM_011543983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC 
TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC 
AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG 
ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA 
CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG 
AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA 
GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC 
TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG 
ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT 
TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG 
CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG 
TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT 
GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC 
CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC 
GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG 
GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT 
GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT 
AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC 
AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG 
GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC 
AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA 
GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC 
AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC 
TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC 
CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA 
TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG 
GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC 
TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT 
CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT 
GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC 
AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT 
GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG 
AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA 
GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG 
GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC 
CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC 
ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT 
GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT 
TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG 
TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG 
AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA 
CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT 
GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA 
TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG 
GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT 
GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT 
GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC 
ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG 
CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC 
AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC 
ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT 
GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC 
AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG 
CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC 
ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AGCTCTGAGG ACAGCTCCCG CATTTCCATT 
ACCTTCTTTC GCCTCTTCCG AGTTATGCGG CTGGTCAAGC TTCTCAGTAA GGGTGAAGGG 
ATCCGCACAT TGCTCTGGAC ATTCATCAAG TCCTTCCAGG CCTTGCCCTA TGTGGCTCTT 
CTCATCGCAA TGATATTCTT CATCTATGCC GTCATTGGCA TGCAGATGTT CGGCAAGGTG 
GCTCTTCAGG ATGGCACACA GATAAACCGA AACAACAACT TCCAGACCTT TCCACAGGCT 
GTGCTGCTTC TGTTCAGGTG TGCCACTGGT GAGGCATGGC AGGAGATAAT GCTTGCCAGC 
CTTCCCGGAA ATCGGTGTGA TCCTGAGTCT GACTTCGGCC CTGGTGAAGA GTTTACCTGT 
GGTAGCAATT TTGCCATCGC CTATTTCATC AGCTTCTTCA TGCTCTGTGC CTTCCTGATC 
ATAAATCTCT TTGTGGCTGT GATCATGGAC AACTTTGATT ATCTCACCAG AGATTGGTCC 
ATCCTGGGCC CCCATCACCT TGATGAATTC AAGAGGATCT GGTCTGAATA TGACCCTGGG 
GCCAAGGGCC GCATCAAACA CTTGGATGTG GTTGCCCTGC TGAGACGTAT CCAGCCCCCT 
CTGGGATTTG GGAAGCTGTG CCCACACCGA GTGGCCTGCA AGAGACTTGT GGCAATGAAC 
ATGCCCCTCA ACTCAGATGG GACGGTGACA TTCAACGCCA CACTCTTTGC CCTGGTCCGG 
ACATCCCTGA AGATCAAAAC AGAAGGGAAC CTGGAGCAAG CCAACCAGGA GCTGCGGATT 
GTCATCAAAA AGATCTGGAA GCGGATGAAA CAGAAGCTGC TAGATGAGGT CATCCCCCCA 
CCAGACGAGG AGGAGGTCAC CGTGGGCAAA TTCTACGCCA CATTTCTGAT CCAGGACTAT 
TTCCGCAAAT TCCGGCGGAG GAAAGAAAAA GGGCTACTAG GCAACGACGC CGCCCCTAGC 
ACCTCTTCCG CCCTTCAGGC TGGTCTGCGG AGCCTGCAGG ACTTGGGTCC TGAGATGCGG 
CAGGCCCTCA CCTGTGACAC AGAGGAGGAG GAAGAAGAGG GGCAGGAGGG AGTGGAGGAG 
GAAGATGAAA AGGACTTGGA AACTAACAAA GCCACGATGG TCTCCCAGCC CTCAGCTCGC 
CGGGGCTCCG GGATTTCTGT GTCTCTGCCT GTCGGGGACA GACTTCCAGA TTCACTCTCC 
TTTGGGCCCA GTGATGATGA CAGGGGGACT CCCACCTCCA GTCAGCCCAG TGTGCCCCAG 
GCTGGATCCA ACACCCACAG GAGAGGCTCT GGGGCTCTCA TTTTCACCAT CCCAGAAGAA 
GGAAATTCTC AGCCCAAGGG AACCAAAGGG CAAAACAAGC AAGATGAGGA TGAGGAAGTC 
CCTGATCGGC TTTCCTACCT AGATGAGCAG GCAGGGACTC CCCCGTGCTC AGTCCTTTTG 
CCACCTCACA GAGCTCAGAG ATACATGGAT GGGCACCTGG TACCACGCCG CCGTCTGCTG 
CCCCCCACAC CTGCAGGTCG GAAGCCCTCC TTCACCATCC AGTGTCTGCA GCGCCAGGGC 
AGTTGTGAGG ATTTACCCAT CCCAGGCACC TATCATCGTG GGCGAAATTC AGGGCCCAAT 
AGGGCTCAGG GTTCCTGGGC AACACCACCT CAGCGGGGTC GGCTCCTGTA TGCCCCGCTG 
TTGTTGGTGG AAGAGGGCGC AGCGGGGGAG GGGTACCTCG GCAGATCCAG TGGCCCACTG 
CGCACCTTCA CCTGTCTGCA CGTGCCTGGA ACCCACTCGG ACCCCAGCCA TGGGAAGAGG 
GGCAGTGCCG ACAGCTTGGT GGAGGCTGTG CTTATCTCAG AGGGTCTGGG CCTCTTTGCT 
CGAGACCCAC GTTTCGTGGC CCTGGCCAAG CAGGAGATTG CAGATGCGTG TCGCCTGACG 
CTGGATGAGA TGGACAATGC TGCCAGTGAC CTGCTGGCAC AGGGAACCAG CTCTCTCTAT 
AGCGACGAGG AGTCCATCCT CTCCCGCTTC GATGAGGAGG ACTTGGGAGA CGAGATGGCC 
TGCGTCCACG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542285.1
CDS	96..5813
Translation	MSESEGGKGERILPSLQTLGASIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNLGSMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNSSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_011543983.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543983.2

ORF Insert Sequence:

ATGTCGGAAT CTGAAGGCGG GAAAGGTGAG AGAATCCTTC CATCCCTGCA GACCCTTGGA 
GCAAGCATCG TGGAGTGGAA GCCCTTCGAC ATCCTCATCC TGCTGACCAT CTTTGCCAAC 
TGCGTGGCCC TGGGAGTTTA CATCCCCTTC CCTGAGGACG ACTCCAACAC TGCCAACCAC 
AACCTGGAGC AGGTGGAGTA CGTATTCCTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG 
ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAATGG CTGGAACCTA 
CTCGACTTCA TCATCGTCGT GGTCGGGCTG TTCAGCGTTC TGCTGGAGCA GGGCCCCGGA 
CGGCCAGGCG ACGCCCCGCA CACCGGGGGA AAGCCAGGAG GCTTCGATGT GAAGGCATTG 
AGGGCGTTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GGGTCCCGAG CCTGCACATA 
GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGCTGC ACATTGCACT GCTCGTGCTC 
TTCGTCATCA TCATTTATGC CATCATTGGG CTCGAGCTGT TCCTTGGACG AATGCACAAG 
ACGTGCTACT TCCTGGGATC CGACATGGAA GCGGAGGAGG ACCCATCGCC CTGTGCGTCT 
TCGGGATCAG GGCGTGCGTG CACGCTGAAC CAGACTGAGT GCCGCGGGCG CTGGCCAGGG 
CCCAATGGAG GCATCACCAA CTTTGACAAC TTCTTCTTCG CCATGCTGAC AGTCTTCCAG 
TGTGTCACCA TGGAAGGCTG GACCGATGTG CTCTACTGGA TGCAAGATGC CATGGGGTAT 
GAACTGCCCT GGGTGTACTT TGTGAGCCTT GTCATCTTTG GGTCCTTCTT CGTCCTCAAC 
CTTGTGCTTG GCGTCCTGAG TGGGGAGTTC TCCAAGGAGA GAGAGAAAGC GAAAGCTCGC 
GGGGACTTCC AGAAGCAGCG GGAGAAGCAG CAGATGGAGG AAGACCTGCG GGGCTACCTG 
GACTGGATCA CTCAAGCCGA AGAGCTGGAC ATGGAGGACC CCTCCGCCGA TGACAACCTT 
GGTTCTATGG CTGAAGAGGG CCGGGCGGGC CATCGGCCAC AGCTGGCCGA GCTGACCAAT 
AGGAGGCGTG GACGTCTGCG CTGGTTCAGT CATTCTACTC GCTCCACACA CTCCACCAGC 
AGCCATGCCA GCCTCCCAGC CAGTGACACC GGTTCCATGA CAGAGACCCA AGGCGATGAG 
GATGAGGAGG AGGGGGCTCT GGCCAGCTGT ACACGCTGCC TAAACAAGAT CATGAAAACC 
AGAGTCTGCC GCCGCCTCCG CCGAGCCAAC CGGGTCCTTC GGGCACGCTG CCGTCGGGCA 
GTGAAGTCCA ATGCCTGCTA CTGGGCTGTG CTGTTGCTCG TCTTCCTCAA CACGTTGACC 
ATCGCCTCTG AGCACCACGG GCAGCCTGTG TGGCTCACCC AGATCCAGGA GTATGCCAAC 
AAAGTGTTGC TCTGTCTGTT CACGGTGGAG ATGCTTCTCA AATTGTACGG TCTGGGCCCC 
TCTGCCTATG TGTCTTCCTT CTTCAACCGC TTTGACTGCT TTGTGGTCTG TGGGGGCATC 
CTAGAGACCA CCTTGGTGGA GGTGGGTGCC ATGCAGCCCT TGGGCATCTC AGTGCTCCGA 
TGTGTGCGCC TCCTCAGGAT CTTTAAGGTC ACCAGACACT GGGCTTCTCT GAGCAATCTG 
GTGGCATCCC TGCTCAATTC AATGAAATCC ATCGCATCCT TGCTGCTTCT CCTCTTCCTC 
TTCATCATTA TCTTCTCCCT GCTTGGCATG CAGCTGTTTG GGGGCAAGTT CAACTTTGAC 
CAGACCCACA CCAAGCGAAG CACCTTTGAC ACGTTCCCCC AGGCCCTCCT CACTGTCTTT 
CAGATCCTGA CAGGTGAGGA CTGGAACGTG GTCATGTATG ATGGTATCAT GGCATATGGT 
GGCCCCTTCT TCCCAGGAAT GTTGGTGTGC ATCTATTTCA TCATTCTCTT CATCTGTGGC 
AACTACATCC TGTTGAACGT GTTTCTTGCC ATTGCTGTGG ACAACCTGGC CAGTGGAGAT 
GCAGGCACTG CCAAGGACAA GGGCGGGGAG AAGAGCAATG AGAAGGATCT CCCACAGGAG 
AATGAAGGCC TGGTGCCTGG TGTGGAGAAA GAGGAAGAGG AGGGTGCAAG GAGGGAAGGA 
GCAGACATGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAAG AAGAGGAAGA AGAGGAAGAG 
GGTGCAGGGG GTGTGGAACT CCTGCAGGAA GTTGTACCCA AGGAGAAGGT GGTACCCATC 
CCTGAGGGCA GCGCCTTCTT CTGCCTCAGC CAAACCAACC CGCTGAGGAA GGGCTGCCAC 
ACCCTCATCC ACCATCATGT CTTCACCAAT CTTATCCTGG TGTTCATCAT CCTCAGCAGT 
GTGTCCCTGG CCGCTGAGGA CCCCATCCGA GCCCACTCCT TCCGCAACCA TATTCTGGGT 
TACTTCGATT ATGCCTTCAC CTCCATTTTC ACTGTGGAGA TTCTACTAAA GATGACAGTG 
TTTGGGGCCT TCCTGCACCG CGGCTCCTTC TGCCGTAGCT GGTTTAATAT GTTGGATCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTT GGCATCCACT CCAGCGCCAT CTCGGTGGTG 
AAGATTCTGC GAGTACTCCG AGTACTGCGG CCCCTCCGAG CCATCAACAG GGCCAAGGGA 
CTCAAGCATG TGGTGCAGTG TGTATTTGTG GCCATCCGGA CCATCGGAAA CATCATGATT 
GTCACCACAC TTCTGCAATT TATGTTCGCC TGCATCGGGG TGCAGCTCTT CAAGGGGAAA 
TTCTACACCT GCACGGACGA GGCCAAACAC ACCCCTCAAG AATGCAAGGG CTCCTTCCTG 
GTATACCCAG ATGGAGACGT GTCACGGCCC CTGGTCCGGG AGCGGCTCTG GGTCAACAGT 
GATTTCAACT TTGACAATGT CCTTTCAGCC ATGATGGCCC TGTTCACTGT CTCCACCTTT 
GAAGGCTGGC CTGCACTGCT ATACAAGGCC ATCGATGCAT ATGCAGAGGA CCACGGCCCC 
ATCTATAATT ACCGTGTGGA GATCTCAGTG TTCTTCATTG TCTACATCAT CATCATTGCG 
TTCTTCATGA TGAACATCTT CGTGGGCTTC GTCATCATCA CTTTCCGTGC CCAGGGCGAG 
CAGGAGTACC AAAACTGTGA GCTGGACAAG AACCAGCGTC AATGTGTGGA ATATGCCCTC 
AAGGCCCAGC CACTCCGCCG TTACATCCCC AAGAACCCGC ATCAGTATCG TGTGTGGGCC 
ACTGTGAACT CTGCTGCCTT TGAGTACCTG ATGTTCCTGC TCATCCTGCT CAACACAGTT 
GCCCTAGCCA TGCAGCACTA TGAGCAGACT GCTCCCTTCA ACTATGCCAT GGACATCCTC 
AACATGGTCT TCACTGGCCT CTTCACTATT GAGATGGTGC TCAAAATCAT CGCCTTCAAG 
CCCAAGCATT ACTTCACTGA TGCCTGGAAC ACGTTTGACG CTCTTATTGT GGTGGGCAGC 
ATAGTGGATA TTGCCGTCAC TGAAGTCAAT AGCTCTGAGG ACAGCTCCCG CATTTCCATT 
ACCTTCTTTC GCCTCTTCCG AGTTATGCGG CTGGTCAAGC TTCTCAGTAA GGGTGAAGGG 
ATCCGCACAT TGCTCTGGAC ATTCATCAAG TCCTTCCAGG CCTTGCCCTA TGTGGCTCTT 
CTCATCGCAA TGATATTCTT CATCTATGCC GTCATTGGCA TGCAGATGTT CGGCAAGGTG 
GCTCTTCAGG ATGGCACACA GATAAACCGA AACAACAACT TCCAGACCTT TCCACAGGCT 
GTGCTGCTTC TGTTCAGGTG TGCCACTGGT GAGGCATGGC AGGAGATAAT GCTTGCCAGC 
CTTCCCGGAA ATCGGTGTGA TCCTGAGTCT GACTTCGGCC CTGGTGAAGA GTTTACCTGT 
GGTAGCAATT TTGCCATCGC CTATTTCATC AGCTTCTTCA TGCTCTGTGC CTTCCTGATC 
ATAAATCTCT TTGTGGCTGT GATCATGGAC AACTTTGATT ATCTCACCAG AGATTGGTCC 
ATCCTGGGCC CCCATCACCT TGATGAATTC AAGAGGATCT GGTCTGAATA TGACCCTGGG 
GCCAAGGGCC GCATCAAACA CTTGGATGTG GTTGCCCTGC TGAGACGTAT CCAGCCCCCT 
CTGGGATTTG GGAAGCTGTG CCCACACCGA GTGGCCTGCA AGAGACTTGT GGCAATGAAC 
ATGCCCCTCA ACTCAGATGG GACGGTGACA TTCAACGCCA CACTCTTTGC CCTGGTCCGG 
ACATCCCTGA AGATCAAAAC AGAAGGGAAC CTGGAGCAAG CCAACCAGGA GCTGCGGATT 
GTCATCAAAA AGATCTGGAA GCGGATGAAA CAGAAGCTGC TAGATGAGGT CATCCCCCCA 
CCAGACGAGG AGGAGGTCAC CGTGGGCAAA TTCTACGCCA CATTTCTGAT CCAGGACTAT 
TTCCGCAAAT TCCGGCGGAG GAAAGAAAAA GGGCTACTAG GCAACGACGC CGCCCCTAGC 
ACCTCTTCCG CCCTTCAGGC TGGTCTGCGG AGCCTGCAGG ACTTGGGTCC TGAGATGCGG 
CAGGCCCTCA CCTGTGACAC AGAGGAGGAG GAAGAAGAGG GGCAGGAGGG AGTGGAGGAG 
GAAGATGAAA AGGACTTGGA AACTAACAAA GCCACGATGG TCTCCCAGCC CTCAGCTCGC 
CGGGGCTCCG GGATTTCTGT GTCTCTGCCT GTCGGGGACA GACTTCCAGA TTCACTCTCC 
TTTGGGCCCA GTGATGATGA CAGGGGGACT CCCACCTCCA GTCAGCCCAG TGTGCCCCAG 
GCTGGATCCA ACACCCACAG GAGAGGCTCT GGGGCTCTCA TTTTCACCAT CCCAGAAGAA 
GGAAATTCTC AGCCCAAGGG AACCAAAGGG CAAAACAAGC AAGATGAGGA TGAGGAAGTC 
CCTGATCGGC TTTCCTACCT AGATGAGCAG GCAGGGACTC CCCCGTGCTC AGTCCTTTTG 
CCACCTCACA GAGCTCAGAG ATACATGGAT GGGCACCTGG TACCACGCCG CCGTCTGCTG 
CCCCCCACAC CTGCAGGTCG GAAGCCCTCC TTCACCATCC AGTGTCTGCA GCGCCAGGGC 
AGTTGTGAGG ATTTACCCAT CCCAGGCACC TATCATCGTG GGCGAAATTC AGGGCCCAAT 
AGGGCTCAGG GTTCCTGGGC AACACCACCT CAGCGGGGTC GGCTCCTGTA TGCCCCGCTG 
TTGTTGGTGG AAGAGGGCGC AGCGGGGGAG GGGTACCTCG GCAGATCCAG TGGCCCACTG 
CGCACCTTCA CCTGTCTGCA CGTGCCTGGA ACCCACTCGG ACCCCAGCCA TGGGAAGAGG 
GGCAGTGCCG ACAGCTTGGT GGAGGCTGTG CTTATCTCAG AGGGTCTGGG CCTCTTTGCT 
CGAGACCCAC GTTTCGTGGC CCTGGCCAAG CAGGAGATTG CAGATGCGTG TCGCCTGACG 
CTGGATGAGA TGGACAATGC TGCCAGTGAC CTGCTGGCAC AGGGAACCAG CTCTCTCTAT 
AGCGACGAGG AGTCCATCCT CTCCCGCTTC GATGAGGAGG ACTTGGGAGA CGAGATGGCC 
TGCGTCCACG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary insights into T-type Ca
The Journal of general physiology149(4)483-510(2017 Apr)
Smith CL,Abdallah S,Wong YY,Le P,Harracksingh AN,Artinian L,Tamvacakis AN,Rehder V,Reese TS,Senatore A

The importance of genetic testing as demonstrated by two cases of
Molecular vision23695-706(2017)
Men CJ,Bujakowska KM,Comander J,Place E,Bedoukian EC,Zhu X,Leroy BP,Fulton AB,Pierce EA

Alternative splicing at C terminus of Ca(V)1.4 calcium channel modulates calcium-dependent inactivation, activation potential, and current density.
The Journal of biological chemistry287(2)832-47(2012 Jan)
Tan GM,Yu D,Wang J,Soong TW

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CACNA1F cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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