MID2 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID2
Entrez Gene ID	102896538
Full Name	midline 2
Synonyms	Midline-2
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025048892.1	XP_024904660.1	probable E3 ubiquitin-protein ligase MID2
XM_015591640.1	XP_015447126.1	probable E3 ubiquitin-protein ligase MID2

Pathways from BioSystems
Gene Ontology

The following MID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt18080	XM_015591640.1 Latest version!	Pteropus alecto midline 2 (MID2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPt88414	XM_025048892.1 Latest version!	Pteropus alecto midline 2 (MID2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID2 ( XM_015591640.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015447126.1 Pteropus alecto midline 2 (MID2), mRNA.

Price & Availability↑

CloneID	OPt18080
Clone ID Related Accession (Same CDS sequence)	XM_015591640.1
Accession Version	XM_015591640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	probable E3 ubiquitin-protein ligase MID2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006437742.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 22% of CDS bases ##RefSeq-Attributes-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATTTGCC TAGAGTTGTT CGAAGATCCC 
CTTCTGCTTC CTTGTGCTCA TAGCCTCTGC TTCAGCTGCG CCCATCGCAT CTTGGTCTCC 
AGCTGCAGCT CTGGTGAATC CATTGAGCCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CGTTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTTTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCA GTTAGTGGGC CTAATTCCCC AAGTGAAAGC 
CGCCGGGAGA GGACTTACAG GCCCAGCACC ACCATGTCTA GTGAACGAAT TGCTTGCCAA 
TTCTGTGAGC AGGATCCGCC AAGGGATGCA GTGAAAACAT GTATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTAGAAC CAGTGCCAGA CACACACCTT CGAGGGATTA CCTGCCTGGA CCATGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ATCGGTTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGTA ACAGCGAGCT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGATA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC GAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAAGCACG GTTTCTCCAG TCTGCAAAAA ATATTGCTGA AAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTACTGG AGGGGCTGGA CTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAATCA TTACACAGTG CACGGACTCC AGAGTGGGAC ACGCTACATC 
TTCATTGTTA AAGCCATAAA CCAAGCAGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ACTGGACCCC AAAATGACTC ACAAGAAATT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTGA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGAGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTAGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCGGCTCCCA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGATGT 
AATAGTAACT TCGTAGTGAG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT TCTGGATTAT GACAACAACA TGCTTTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC TCTAATGATC CTTGGGGAAG GCGGACGTGA CACGTTTGAA 
AATCGGAGGC AAACTATTTA CCATAACAAA AAGAAAAAAA AAAAGCGCAG ACGGTTTGCA 
TGCACACCTG GGTTTGCTGT CTTCAGACAA AGCCCACCAA GTCCATCCTA TAAAGTCACA 
CACGTGAGAG AAATAAGCCA TATTGCTAAC TTTAGTGCTG CTGACTTGAT AAGTAACGCT 
TTTCAAGGTA TGGATGGACT AATCCTGGAC ATTATTCTAA TATATCCAGA GAAACTTCTG 
TTCATTCTTG ATGGATTTCC TGAGCTTCAG TATCCTATAG ATGACCAGGA AGAGGATCTT 
AGTGCTAACC CTGAAGAGAG GAAGCCAGTA GAGACCCTTT TACGTAGTTT CGTGAGGAAA 
AAACTGTTCC CCAAATCCTC CCTCCTGATA ACTGCCAGGC CTACAACCAT GAAGAAGCTC 
CACTCTTTGT TAAAACAACC TATCCAGACA GAGATCCTGT GGTTTACAGA TGCTGAAAAG 
AGAGCATATT TCTTGAGTCA ATTTTCAGTT GCTAATGCAG CAATGAGAGT CTTCTATGGT 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015447126.1
CDS	131..2656
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTTMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELIEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILGEGGRDTFENRRQTIYHNKKKKKKRRRFACTPGFAVFRQSPPSPSYKVTHVREISHIANFSAADLISNAFQGMDGLILDIILIYPEKLLFILDGFPELQYPIDDQEEDLSANPEERKPVETLLRSFVRKKLFPKSSLLITARPTTMKKLHSLLKQPIQTEILWFTDAEKRAYFLSQFSVANAAMRVFYGL

Target ORF information:

RefSeq Version	XM_015591640.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto midline 2 (MID2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015591640.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATTTGCC TAGAGTTGTT CGAAGATCCC 
CTTCTGCTTC CTTGTGCTCA TAGCCTCTGC TTCAGCTGCG CCCATCGCAT CTTGGTCTCC 
AGCTGCAGCT CTGGTGAATC CATTGAGCCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CGTTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTTTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCA GTTAGTGGGC CTAATTCCCC AAGTGAAAGC 
CGCCGGGAGA GGACTTACAG GCCCAGCACC ACCATGTCTA GTGAACGAAT TGCTTGCCAA 
TTCTGTGAGC AGGATCCGCC AAGGGATGCA GTGAAAACAT GTATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTAGAAC CAGTGCCAGA CACACACCTT CGAGGGATTA CCTGCCTGGA CCATGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ATCGGTTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGTA ACAGCGAGCT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGATA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC GAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAAGCACG GTTTCTCCAG TCTGCAAAAA ATATTGCTGA AAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTACTGG AGGGGCTGGA CTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAATCA TTACACAGTG CACGGACTCC AGAGTGGGAC ACGCTACATC 
TTCATTGTTA AAGCCATAAA CCAAGCAGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ACTGGACCCC AAAATGACTC ACAAGAAATT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTGA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGAGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTAGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCGGCTCCCA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGATGT 
AATAGTAACT TCGTAGTGAG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT TCTGGATTAT GACAACAACA TGCTTTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC TCTAATGATC CTTGGGGAAG GCGGACGTGA CACGTTTGAA 
AATCGGAGGC AAACTATTTA CCATAACAAA AAGAAAAAAA AAAAGCGCAG ACGGTTTGCA 
TGCACACCTG GGTTTGCTGT CTTCAGACAA AGCCCACCAA GTCCATCCTA TAAAGTCACA 
CACGTGAGAG AAATAAGCCA TATTGCTAAC TTTAGTGCTG CTGACTTGAT AAGTAACGCT 
TTTCAAGGTA TGGATGGACT AATCCTGGAC ATTATTCTAA TATATCCAGA GAAACTTCTG 
TTCATTCTTG ATGGATTTCC TGAGCTTCAG TATCCTATAG ATGACCAGGA AGAGGATCTT 
AGTGCTAACC CTGAAGAGAG GAAGCCAGTA GAGACCCTTT TACGTAGTTT CGTGAGGAAA 
AAACTGTTCC CCAAATCCTC CCTCCTGATA ACTGCCAGGC CTACAACCAT GAAGAAGCTC 
CACTCTTTGT TAAAACAACC TATCCAGACA GAGATCCTGT GGTTTACAGA TGCTGAAAAG 
AGAGCATATT TCTTGAGTCA ATTTTCAGTT GCTAATGCAG CAATGAGAGT CTTCTATGGT 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_025048892.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_024904660.1 Pteropus alecto midline 2 (MID2), mRNA.

Price & Availability↑

CloneID	OPt88414
Clone ID Related Accession (Same CDS sequence)	XM_025048892.1
Accession Version	XM_025048892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	probable E3 ubiquitin-protein ligase MID2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006437742.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases ##RefSeq-Attributes-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATTTGCC TAGAGTTGTT CGAAGATCCC 
CTTCTGCTTC CTTGTGCTCA TAGCCTCTGC TTCAGCTGCG CCCATCGCAT CTTGGTCTCC 
AGCTGCAGCT CTGGTGAATC CATTGAGCCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CGTTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTTTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCA GTTAGTGGGC CTAATTCCCC AAGTGAAAGC 
CGCCGGGAGA GGACTTACAG GCCCAGCACC ACCATGTCTA GTGAACGAAT TGCTTGCCAA 
TTCTGTGAGC AGGATCCGCC AAGGGATGCA GTGAAAACAT GTATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTAGAAC CAGTGCCAGA CACACACCTT CGAGGGATTA CCTGCCTGGA CCATGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ATCGGTTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGTA ACAGCGAGCT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGATA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC GAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAAGCACG GTTTCTCCAG TCTGCAAAAA ATATTGCTGA AAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTACTGG AGGGGCTGGA CTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAATCA TTACACAGTG CACGGACTCC AGAGTGGGAC ACGCTACATC 
TTCATTGTTA AAGCCATAAA CCAAGCAGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ACTGGACCCC AAAATGACTC ACAAGAAATT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTGA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGAGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTAGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCGGCTCCCA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGATGT 
AATAGTAACT TCGTAGTGAG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT TCTGGATTAT GACAACAACA TGCTTTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC TCTAATGATC CTGTTAGATA ATCATTCCTC TTGCCACCTT 
GTTTACACTC TCATCTGCTT AGCCAACACT TCATGGCAAA TGCAGATGTC AACTTTATCC 
CAGTCCTGGT TGAAGGGAAC CAAACTCTAT GGTCTATGCT TTTGTCAGTT CCACTGCAGG 
GATCAGAGAT GTCAGAACCG CAACTTTCTC CTTTCCAGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024904660.1
CDS	166..2310
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTTMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELIEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILLDNHSSCHLVYTLICLANTSWQMQMSTLSQSWLKGTKLYGLCFCQFHCRDQRCQNRNFLLSRS

Target ORF information:

RefSeq Version	XM_025048892.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto midline 2 (MID2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025048892.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATTTGCC TAGAGTTGTT CGAAGATCCC 
CTTCTGCTTC CTTGTGCTCA TAGCCTCTGC TTCAGCTGCG CCCATCGCAT CTTGGTCTCC 
AGCTGCAGCT CTGGTGAATC CATTGAGCCC ATTACTGCTT TCCAGTGTCC TACGTGCAGG 
TATGTTATCT CGTTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTTTGCAG 
AACATTATTG ACCGCTTCCA GAAGGCTTCA GTTAGTGGGC CTAATTCCCC AAGTGAAAGC 
CGCCGGGAGA GGACTTACAG GCCCAGCACC ACCATGTCTA GTGAACGAAT TGCTTGCCAA 
TTCTGTGAGC AGGATCCGCC AAGGGATGCA GTGAAAACAT GTATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTAGAAC CAGTGCCAGA CACACACCTT CGAGGGATTA CCTGCCTGGA CCATGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGGCGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ATCGGTTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGTA ACAGCGAGCT AGAAAATCAA 
ATGGCCAAGC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAATTGATA GAGATCATCC AGCAGAGGAA GCAAATGATT 
GCTGTCAAAA TCAAAGAGAC GAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAAGCACG GTTTCTCCAG TCTGCAAAAA ATATTGCTGA AAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTACTGG AGGGGCTGGA CTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAATCA TTACACAGTG CACGGACTCC AGAGTGGGAC ACGCTACATC 
TTCATTGTTA AAGCCATAAA CCAAGCAGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ACTGGACCCC AAAATGACTC ACAAGAAATT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTGA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGAGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTAGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCGGCTCCCA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGATGT 
AATAGTAACT TCGTAGTGAG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT TCTGGATTAT GACAACAACA TGCTTTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC TCTAATGATC CTGTTAGATA ATCATTCCTC TTGCCACCTT 
GTTTACACTC TCATCTGCTT AGCCAACACT TCATGGCAAA TGCAGATGTC AACTTTATCC 
CAGTCCTGGT TGAAGGGAAC CAAACTCTAT GGTCTATGCT TTTGTCAGTT CCACTGCAGG 
GATCAGAGAT GTCAGAACCG CAACTTTCTC CTTTCCAGGA GCTGA