N4BP1 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	N4BP1
Entrez Gene ID	102448645
Full Name	NEDD4 binding protein 1
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006121241.3	XP_006121303.1	NEDD4-binding protein 1
XM_006121241.2	XP_006121303.1	NEDD4-binding protein 1

Pathways from BioSystems
Gene Ontology

The following N4BP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N4BP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe27526	XM_006121241.3 Latest version!	Pelodiscus sinensis NEDD4 binding protein 1 (N4BP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OPe27526	XM_006121241.2	Pelodiscus sinensis NEDD4 binding protein 1 (N4BP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

N4BP1 ( XM_006121241.3 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006121303.1 Pelodiscus sinensis NEDD4 binding protein 1 (N4BP1), mRNA.

Price & Availability↑

CloneID	OPe27526
Clone ID Related Accession (Same CDS sequence)	XM_006121241.3 , XM_006121241.2
Accession Version	XM_006121241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	NEDD4-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854622.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006121241.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases ##RefSeq-Attributes-END##

ATGATTGAAA GAAGAAAACA CCCCTCTGGG GGCAGCATTC AGAAAAAGAA AGAAGCAAAG 
GAAGCTTTTC TATCTAAGCA GGAAGGTGCT CTCCTGAGAC ACACAGACAC AAATGTTCAC 
AGTGAGCCTT CTGGCCCCAG TGAGGATGTG AGTGGTGAGG AGATTCCTGA TCTTCCAGTT 
AGTCAGAGTG CAGGTGACCT GGCACTTACT GCAGCATCCA TATCTCCATC TCAAATGGAT 
GTAACCATGC ACATTCCTGA AGAAAAGTGT AAATCAGAGA ACAGAAAAGA GTGTGAACCT 
AGTGAAAATG TCCCTACAAT GGTGACTGTC AGAGAGAATT TTCTAGAATT TATTGACATT 
GATGATACTA CAGGAGCTGG TATGACAAAT GTGCTTCTTA AAAAGCTGAA AGATATGGGA 
ATTGCGATAG CTGACATGAG AGGGCAGGTC TATTATAATG GTGCCAACAT GAGAGGAAAG 
AACAGAGAAG TGCAGAAACA GATTCAAGAG TTAAACCCTC AAGCTTTTTT TGTCCCATGC 
TGTTCTCATT CATTGAACTT GGTGGAATAT ATTAAGGGAC TTTGTGAACC TGAATTAGAA 
GAAAAGGAAT ATTATCCAAA GGACATGCAC TGCATTTTCG TTGGCGCACA GAGCCTGTTT 
CTTAACTGTC TTATTCAGGA TACCTGTGCA GATATAACAG TGCAGGAAAT AGGATTGCTT 
ATTATTAAGG GTGGTGCAGA AGCTGTTGTA ATGGCTAGAA GTCATATTCA GCAATTTGTG 
AAACTCTTTA AAAACAATGA AAGCTTATTA AGTGACAAGG AATCAAATGT AAAAAAACAA 
TTTAAACAAT TTGTGGAAGC ACATGCAGAT AAATATACAA TGGACTTGTT GATCTTGCCT 
AGCTCACTAA AAAGAGAACT TCTGAATCTT ACACAAAGTG ATTGTTGTGA AGCAGATGGC 
AATATTATAG ATCTTACTAC AGATTTGGTA AGCACATCTG AATTTTCACA GAACAAAACC 
CCAAAAATAA CGATGAGTGG AGATGGAAGA ACTGTTGGGG AGGAAGCAAG AAATAAAGCT 
GGAACCCCTG TAACAGAGCT TACAAAACAA ATGGATACAG TGTTTTCCAA TGCACCAGGA 
AGAGGTTTTA TTCCTATAAG TGGTGTGACT CCACTTGAGA CATCTCTTTC CAAAGAGAGA 
CAGTCATGTA AAAGAAGGTG TTCTGATGCT GAGGAAAGGC TTCCTAAGAA ACAGTTTTCA 
CTAGAAAGTG ATCAGGAGGG CAGGCCTACA CAATGTAGTG AAGATCCAGA TGGGAGAGCA 
GTTGTTGATC TGACTTCTGA TAATTGCAGT GAATTGGATG ATCCACGTTT CTCTATTAAA 
GACTGCGAAG ACATTAGTGA GGAAATGGAA TACAAGATTC TTGTAAACTT TTTCAGGACC 
ATGGGATATT CCCAAAAAAT TGTAGAGAAA GTTATTAGTG AACTGGGACA ATCAGTGGAA 
CCATTAATGC TCCTGGAAGC AATAGAAAAG GAAAGTACAA AGTTTCAGAA GGAAAAAGAA 
CAGTCACAGC AAATATCTAG ATCTGTAAGT GTGCATTCAG GTGGTAATGC AGATCTTTCT 
CAGCCTCTGC AATATAGAGA TTCTGTCAGT AAAAATCAAC TTAAACCAGG TCATACTTTA 
AAGGAGATAA AAAATGTAAA ACAAAGAGAA AATTCTCCAT GTGCACGTCT TCTGCCAGAA 
GACAAAATGC ATGCGTCAAG TTATAAAAAT AAAATTGCTG TTCCTTGTGG TAAAAATTCA 
GCAGAGCATT GTAAACAAGT ACACTTGAAT TCTTTTGATC AAAGTCCCAG GACAAATGAT 
ATAGAGACTG ATGGTCTTGT ACCTTCTTCT AACATTTCAC AAGAATTCAT GAAAAATGGA 
GCACTAAAAA ATGTTGACTT TGTTGCAAGA GGGAGCTCAG ACAGAGCATG TGAATCAGTT 
CAGACTGAAA CTGTAAACCA ACAAAAATCT ACACAGCTCT CGAATACACA GAATAGTCAA 
CCTGATCAAA TGGCGAGACC AGGACATTGC AATCCTTCTC AGCTTAGATT CCTCCACCAA 
CCCTCTTTTG ATATCCACAA ACCTGTTGAA TGTACTTTTC CAAACTCTGT ATTTCCATCC 
CATCCACATG CTCCAAATCT AGAGAGGAAG CCAGCTGGAC CATACAATCA TCATACAGAT 
GCCTCAGTTA CTGGGATTCA AAGATTTATG GATTCTCTGA AAAAGCCATA CAATTTGGAA 
TTGAAAAATG AACCTGGGAA AGCATCTTTA AAATACATCA TTATAGATGG GAGCAACGTT 
GCAATTTCTC ATGGTCTACA GAAGTTCTTC TCCTGTCGAG GAATTGCAAT AGCTGTTGAC 
TATTTCTGGA AACGTGGCCA CAGAAACATT ACGGTGTTTG TTCCACAGTG GAGAACAAGA 
CGTGATCCTA ACATCAAAGA GCAGCATTTC TTAACACAGC TCCAGGATGT TGGAATATTA 
TCTCTAACTC CTGCAAGGAT GGTATTGGGA GCAAGAATTG CTGCTCATGA TGACAGATTT 
TTGCTGCATC TTGCTGATAA AACTGGAGGT ATTATTGTTA CCAATGATAA TTTCAGAGAA 
TTTGTGACGG AATCGTATTC TTGGAGAGAG ATTATTCAAA AAAGGTTGCT CCAATATACA 
TTTGCTGGGG ACATATTCAT GGTTCCTGAT GATCCTTTGG GAAGAAATGG ACCTAGATTA 
GAGGACTTCC TTCGGAGTGA AGGCTGTTCT AGAGATTTTC CATTATCACA AATAACTCTA 
CCAACTGGGG GACTGCATTC TTCTGGGACC TCACACTTCA TGCCAATACC CAAGTCATGC 
AGTAATAGAC AACCTAAGAC TGAAGTACGT GGCGGTCCTC CATCTGCCTG GCCTCCGTTG 
GAACCAAACT TTCCAAATGT ACACACTGGT CTTACAATTC CCCCACAAAG ATCTTCTACA 
GAAACAATAC AGCTAAGGGA AGCGTTGACA AAAATATTTC CCGATTCTGA GCAGAGACAG 
AAGATTGATC GAATACTAGC TGAACATCCA TTCATGAGAG ACCTTAATGC ACTGTCTGCC 
ATGGTACTTG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006121303.1
CDS	1..3135
Translation	MIERRKHPSGGSIQKKKEAKEAFLSKQEGALLRHTDTNVHSEPSGPSEDVSGEEIPDLPVSQSAGDLALTAASISPSQMDVTMHIPEEKCKSENRKECEPSENVPTMVTVRENFLEFIDIDDTTGAGMTNVLLKKLKDMGIAIADMRGQVYYNGANMRGKNREVQKQIQELNPQAFFVPCCSHSLNLVEYIKGLCEPELEEKEYYPKDMHCIFVGAQSLFLNCLIQDTCADITVQEIGLLIIKGGAEAVVMARSHIQQFVKLFKNNESLLSDKESNVKKQFKQFVEAHADKYTMDLLILPSSLKRELLNLTQSDCCEADGNIIDLTTDLVSTSEFSQNKTPKITMSGDGRTVGEEARNKAGTPVTELTKQMDTVFSNAPGRGFIPISGVTPLETSLSKERQSCKRRCSDAEERLPKKQFSLESDQEGRPTQCSEDPDGRAVVDLTSDNCSELDDPRFSIKDCEDISEEMEYKILVNFFRTMGYSQKIVEKVISELGQSVEPLMLLEAIEKESTKFQKEKEQSQQISRSVSVHSGGNADLSQPLQYRDSVSKNQLKPGHTLKEIKNVKQRENSPCARLLPEDKMHASSYKNKIAVPCGKNSAEHCKQVHLNSFDQSPRTNDIETDGLVPSSNISQEFMKNGALKNVDFVARGSSDRACESVQTETVNQQKSTQLSNTQNSQPDQMARPGHCNPSQLRFLHQPSFDIHKPVECTFPNSVFPSHPHAPNLERKPAGPYNHHTDASVTGIQRFMDSLKKPYNLELKNEPGKASLKYIIIDGSNVAISHGLQKFFSCRGIAIAVDYFWKRGHRNITVFVPQWRTRRDPNIKEQHFLTQLQDVGILSLTPARMVLGARIAAHDDRFLLHLADKTGGIIVTNDNFREFVTESYSWREIIQKRLLQYTFAGDIFMVPDDPLGRNGPRLEDFLRSEGCSRDFPLSQITLPTGGLHSSGTSHFMPIPKSCSNRQPKTEVRGGPPSAWPPLEPNFPNVHTGLTIPPQRSSTETIQLREALTKIFPDSEQRQKIDRILAEHPFMRDLNALSAMVLD

Target ORF information:

RefSeq Version	XM_006121241.3
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis NEDD4 binding protein 1 (N4BP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006121241.3

ORF Insert Sequence:

ATGATTGAAA GAAGAAAACA CCCCTCTGGG GGCAGCATTC AGAAAAAGAA AGAAGCAAAG 
GAAGCTTTTC TATCTAAGCA GGAAGGTGCT CTCCTGAGAC ACACAGACAC AAATGTTCAC 
AGTGAGCCTT CTGGCCCCAG TGAGGATGTG AGTGGTGAGG AGATTCCTGA TCTTCCAGTT 
AGTCAGAGTG CAGGTGACCT GGCACTTACT GCAGCATCCA TATCTCCATC TCAAATGGAT 
GTAACCATGC ACATTCCTGA AGAAAAGTGT AAATCAGAGA ACAGAAAAGA GTGTGAACCT 
AGTGAAAATG TCCCTACAAT GGTGACTGTC AGAGAGAATT TTCTAGAATT TATTGACATT 
GATGATACTA CAGGAGCTGG TATGACAAAT GTGCTTCTTA AAAAGCTGAA AGATATGGGA 
ATTGCGATAG CTGACATGAG AGGGCAGGTC TATTATAATG GTGCCAACAT GAGAGGAAAG 
AACAGAGAAG TGCAGAAACA GATTCAAGAG TTAAACCCTC AAGCTTTTTT TGTCCCATGC 
TGTTCTCATT CATTGAACTT GGTGGAATAT ATTAAGGGAC TTTGTGAACC TGAATTAGAA 
GAAAAGGAAT ATTATCCAAA GGACATGCAC TGCATTTTCG TTGGCGCACA GAGCCTGTTT 
CTTAACTGTC TTATTCAGGA TACCTGTGCA GATATAACAG TGCAGGAAAT AGGATTGCTT 
ATTATTAAGG GTGGTGCAGA AGCTGTTGTA ATGGCTAGAA GTCATATTCA GCAATTTGTG 
AAACTCTTTA AAAACAATGA AAGCTTATTA AGTGACAAGG AATCAAATGT AAAAAAACAA 
TTTAAACAAT TTGTGGAAGC ACATGCAGAT AAATATACAA TGGACTTGTT GATCTTGCCT 
AGCTCACTAA AAAGAGAACT TCTGAATCTT ACACAAAGTG ATTGTTGTGA AGCAGATGGC 
AATATTATAG ATCTTACTAC AGATTTGGTA AGCACATCTG AATTTTCACA GAACAAAACC 
CCAAAAATAA CGATGAGTGG AGATGGAAGA ACTGTTGGGG AGGAAGCAAG AAATAAAGCT 
GGAACCCCTG TAACAGAGCT TACAAAACAA ATGGATACAG TGTTTTCCAA TGCACCAGGA 
AGAGGTTTTA TTCCTATAAG TGGTGTGACT CCACTTGAGA CATCTCTTTC CAAAGAGAGA 
CAGTCATGTA AAAGAAGGTG TTCTGATGCT GAGGAAAGGC TTCCTAAGAA ACAGTTTTCA 
CTAGAAAGTG ATCAGGAGGG CAGGCCTACA CAATGTAGTG AAGATCCAGA TGGGAGAGCA 
GTTGTTGATC TGACTTCTGA TAATTGCAGT GAATTGGATG ATCCACGTTT CTCTATTAAA 
GACTGCGAAG ACATTAGTGA GGAAATGGAA TACAAGATTC TTGTAAACTT TTTCAGGACC 
ATGGGATATT CCCAAAAAAT TGTAGAGAAA GTTATTAGTG AACTGGGACA ATCAGTGGAA 
CCATTAATGC TCCTGGAAGC AATAGAAAAG GAAAGTACAA AGTTTCAGAA GGAAAAAGAA 
CAGTCACAGC AAATATCTAG ATCTGTAAGT GTGCATTCAG GTGGTAATGC AGATCTTTCT 
CAGCCTCTGC AATATAGAGA TTCTGTCAGT AAAAATCAAC TTAAACCAGG TCATACTTTA 
AAGGAGATAA AAAATGTAAA ACAAAGAGAA AATTCTCCAT GTGCACGTCT TCTGCCAGAA 
GACAAAATGC ATGCGTCAAG TTATAAAAAT AAAATTGCTG TTCCTTGTGG TAAAAATTCA 
GCAGAGCATT GTAAACAAGT ACACTTGAAT TCTTTTGATC AAAGTCCCAG GACAAATGAT 
ATAGAGACTG ATGGTCTTGT ACCTTCTTCT AACATTTCAC AAGAATTCAT GAAAAATGGA 
GCACTAAAAA ATGTTGACTT TGTTGCAAGA GGGAGCTCAG ACAGAGCATG TGAATCAGTT 
CAGACTGAAA CTGTAAACCA ACAAAAATCT ACACAGCTCT CGAATACACA GAATAGTCAA 
CCTGATCAAA TGGCGAGACC AGGACATTGC AATCCTTCTC AGCTTAGATT CCTCCACCAA 
CCCTCTTTTG ATATCCACAA ACCTGTTGAA TGTACTTTTC CAAACTCTGT ATTTCCATCC 
CATCCACATG CTCCAAATCT AGAGAGGAAG CCAGCTGGAC CATACAATCA TCATACAGAT 
GCCTCAGTTA CTGGGATTCA AAGATTTATG GATTCTCTGA AAAAGCCATA CAATTTGGAA 
TTGAAAAATG AACCTGGGAA AGCATCTTTA AAATACATCA TTATAGATGG GAGCAACGTT 
GCAATTTCTC ATGGTCTACA GAAGTTCTTC TCCTGTCGAG GAATTGCAAT AGCTGTTGAC 
TATTTCTGGA AACGTGGCCA CAGAAACATT ACGGTGTTTG TTCCACAGTG GAGAACAAGA 
CGTGATCCTA ACATCAAAGA GCAGCATTTC TTAACACAGC TCCAGGATGT TGGAATATTA 
TCTCTAACTC CTGCAAGGAT GGTATTGGGA GCAAGAATTG CTGCTCATGA TGACAGATTT 
TTGCTGCATC TTGCTGATAA AACTGGAGGT ATTATTGTTA CCAATGATAA TTTCAGAGAA 
TTTGTGACGG AATCGTATTC TTGGAGAGAG ATTATTCAAA AAAGGTTGCT CCAATATACA 
TTTGCTGGGG ACATATTCAT GGTTCCTGAT GATCCTTTGG GAAGAAATGG ACCTAGATTA 
GAGGACTTCC TTCGGAGTGA AGGCTGTTCT AGAGATTTTC CATTATCACA AATAACTCTA 
CCAACTGGGG GACTGCATTC TTCTGGGACC TCACACTTCA TGCCAATACC CAAGTCATGC 
AGTAATAGAC AACCTAAGAC TGAAGTACGT GGCGGTCCTC CATCTGCCTG GCCTCCGTTG 
GAACCAAACT TTCCAAATGT ACACACTGGT CTTACAATTC CCCCACAAAG ATCTTCTACA 
GAAACAATAC AGCTAAGGGA AGCGTTGACA AAAATATTTC CCGATTCTGA GCAGAGACAG 
AAGATTGATC GAATACTAGC TGAACATCCA TTCATGAGAG ACCTTAATGC ACTGTCTGCC 
ATGGTACTTG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP1 ( XM_006121241.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006121303.1 Pelodiscus sinensis NEDD4 binding protein 1 (N4BP1), mRNA.

Price & Availability↑

CloneID	OPe27526
Clone ID Related Accession (Same CDS sequence)	XM_006121241.3 , XM_006121241.2
Accession Version	XM_006121241.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	NEDD4-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854622.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006121241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases ##RefSeq-Attributes-END##

ATGATTGAAA GAAGAAAACA CCCCTCTGGG GGCAGCATTC AGAAAAAGAA AGAAGCAAAG 
GAAGCTTTTC TATCTAAGCA GGAAGGTGCT CTCCTGAGAC ACACAGACAC AAATGTTCAC 
AGTGAGCCTT CTGGCCCCAG TGAGGATGTG AGTGGTGAGG AGATTCCTGA TCTTCCAGTT 
AGTCAGAGTG CAGGTGACCT GGCACTTACT GCAGCATCCA TATCTCCATC TCAAATGGAT 
GTAACCATGC ACATTCCTGA AGAAAAGTGT AAATCAGAGA ACAGAAAAGA GTGTGAACCT 
AGTGAAAATG TCCCTACAAT GGTGACTGTC AGAGAGAATT TTCTAGAATT TATTGACATT 
GATGATACTA CAGGAGCTGG TATGACAAAT GTGCTTCTTA AAAAGCTGAA AGATATGGGA 
ATTGCGATAG CTGACATGAG AGGGCAGGTC TATTATAATG GTGCCAACAT GAGAGGAAAG 
AACAGAGAAG TGCAGAAACA GATTCAAGAG TTAAACCCTC AAGCTTTTTT TGTCCCATGC 
TGTTCTCATT CATTGAACTT GGTGGAATAT ATTAAGGGAC TTTGTGAACC TGAATTAGAA 
GAAAAGGAAT ATTATCCAAA GGACATGCAC TGCATTTTCG TTGGCGCACA GAGCCTGTTT 
CTTAACTGTC TTATTCAGGA TACCTGTGCA GATATAACAG TGCAGGAAAT AGGATTGCTT 
ATTATTAAGG GTGGTGCAGA AGCTGTTGTA ATGGCTAGAA GTCATATTCA GCAATTTGTG 
AAACTCTTTA AAAACAATGA AAGCTTATTA AGTGACAAGG AATCAAATGT AAAAAAACAA 
TTTAAACAAT TTGTGGAAGC ACATGCAGAT AAATATACAA TGGACTTGTT GATCTTGCCT 
AGCTCACTAA AAAGAGAACT TCTGAATCTT ACACAAAGTG ATTGTTGTGA AGCAGATGGC 
AATATTATAG ATCTTACTAC AGATTTGGTA AGCACATCTG AATTTTCACA GAACAAAACC 
CCAAAAATAA CGATGAGTGG AGATGGAAGA ACTGTTGGGG AGGAAGCAAG AAATAAAGCT 
GGAACCCCTG TAACAGAGCT TACAAAACAA ATGGATACAG TGTTTTCCAA TGCACCAGGA 
AGAGGTTTTA TTCCTATAAG TGGTGTGACT CCACTTGAGA CATCTCTTTC CAAAGAGAGA 
CAGTCATGTA AAAGAAGGTG TTCTGATGCT GAGGAAAGGC TTCCTAAGAA ACAGTTTTCA 
CTAGAAAGTG ATCAGGAGGG CAGGCCTACA CAATGTAGTG AAGATCCAGA TGGGAGAGCA 
GTTGTTGATC TGACTTCTGA TAATTGCAGT GAATTGGATG ATCCACGTTT CTCTATTAAA 
GACTGCGAAG ACATTAGTGA GGAAATGGAA TACAAGATTC TTGTAAACTT TTTCAGGACC 
ATGGGATATT CCCAAAAAAT TGTAGAGAAA GTTATTAGTG AACTGGGACA ATCAGTGGAA 
CCATTAATGC TCCTGGAAGC AATAGAAAAG GAAAGTACAA AGTTTCAGAA GGAAAAAGAA 
CAGTCACAGC AAATATCTAG ATCTGTAAGT GTGCATTCAG GTGGTAATGC AGATCTTTCT 
CAGCCTCTGC AATATAGAGA TTCTGTCAGT AAAAATCAAC TTAAACCAGG TCATACTTTA 
AAGGAGATAA AAAATGTAAA ACAAAGAGAA AATTCTCCAT GTGCACGTCT TCTGCCAGAA 
GACAAAATGC ATGCGTCAAG TTATAAAAAT AAAATTGCTG TTCCTTGTGG TAAAAATTCA 
GCAGAGCATT GTAAACAAGT ACACTTGAAT TCTTTTGATC AAAGTCCCAG GACAAATGAT 
ATAGAGACTG ATGGTCTTGT ACCTTCTTCT AACATTTCAC AAGAATTCAT GAAAAATGGA 
GCACTAAAAA ATGTTGACTT TGTTGCAAGA GGGAGCTCAG ACAGAGCATG TGAATCAGTT 
CAGACTGAAA CTGTAAACCA ACAAAAATCT ACACAGCTCT CGAATACACA GAATAGTCAA 
CCTGATCAAA TGGCGAGACC AGGACATTGC AATCCTTCTC AGCTTAGATT CCTCCACCAA 
CCCTCTTTTG ATATCCACAA ACCTGTTGAA TGTACTTTTC CAAACTCTGT ATTTCCATCC 
CATCCACATG CTCCAAATCT AGAGAGGAAG CCAGCTGGAC CATACAATCA TCATACAGAT 
GCCTCAGTTA CTGGGATTCA AAGATTTATG GATTCTCTGA AAAAGCCATA CAATTTGGAA 
TTGAAAAATG AACCTGGGAA AGCATCTTTA AAATACATCA TTATAGATGG GAGCAACGTT 
GCAATTTCTC ATGGTCTACA GAAGTTCTTC TCCTGTCGAG GAATTGCAAT AGCTGTTGAC 
TATTTCTGGA AACGTGGCCA CAGAAACATT ACGGTGTTTG TTCCACAGTG GAGAACAAGA 
CGTGATCCTA ACATCAAAGA GCAGCATTTC TTAACACAGC TCCAGGATGT TGGAATATTA 
TCTCTAACTC CTGCAAGGAT GGTATTGGGA GCAAGAATTG CTGCTCATGA TGACAGATTT 
TTGCTGCATC TTGCTGATAA AACTGGAGGT ATTATTGTTA CCAATGATAA TTTCAGAGAA 
TTTGTGACGG AATCGTATTC TTGGAGAGAG ATTATTCAAA AAAGGTTGCT CCAATATACA 
TTTGCTGGGG ACATATTCAT GGTTCCTGAT GATCCTTTGG GAAGAAATGG ACCTAGATTA 
GAGGACTTCC TTCGGAGTGA AGGCTGTTCT AGAGATTTTC CATTATCACA AATAACTCTA 
CCAACTGGGG GACTGCATTC TTCTGGGACC TCACACTTCA TGCCAATACC CAAGTCATGC 
AGTAATAGAC AACCTAAGAC TGAAGTACGT GGCGGTCCTC CATCTGCCTG GCCTCCGTTG 
GAACCAAACT TTCCAAATGT ACACACTGGT CTTACAATTC CCCCACAAAG ATCTTCTACA 
GAAACAATAC AGCTAAGGGA AGCGTTGACA AAAATATTTC CCGATTCTGA GCAGAGACAG 
AAGATTGATC GAATACTAGC TGAACATCCA TTCATGAGAG ACCTTAATGC ACTGTCTGCC 
ATGGTACTTG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006121303.1
CDS	1..3135
Translation	MIERRKHPSGGSIQKKKEAKEAFLSKQEGALLRHTDTNVHSEPSGPSEDVSGEEIPDLPVSQSAGDLALTAASISPSQMDVTMHIPEEKCKSENRKECEPSENVPTMVTVRENFLEFIDIDDTTGAGMTNVLLKKLKDMGIAIADMRGQVYYNGANMRGKNREVQKQIQELNPQAFFVPCCSHSLNLVEYIKGLCEPELEEKEYYPKDMHCIFVGAQSLFLNCLIQDTCADITVQEIGLLIIKGGAEAVVMARSHIQQFVKLFKNNESLLSDKESNVKKQFKQFVEAHADKYTMDLLILPSSLKRELLNLTQSDCCEADGNIIDLTTDLVSTSEFSQNKTPKITMSGDGRTVGEEARNKAGTPVTELTKQMDTVFSNAPGRGFIPISGVTPLETSLSKERQSCKRRCSDAEERLPKKQFSLESDQEGRPTQCSEDPDGRAVVDLTSDNCSELDDPRFSIKDCEDISEEMEYKILVNFFRTMGYSQKIVEKVISELGQSVEPLMLLEAIEKESTKFQKEKEQSQQISRSVSVHSGGNADLSQPLQYRDSVSKNQLKPGHTLKEIKNVKQRENSPCARLLPEDKMHASSYKNKIAVPCGKNSAEHCKQVHLNSFDQSPRTNDIETDGLVPSSNISQEFMKNGALKNVDFVARGSSDRACESVQTETVNQQKSTQLSNTQNSQPDQMARPGHCNPSQLRFLHQPSFDIHKPVECTFPNSVFPSHPHAPNLERKPAGPYNHHTDASVTGIQRFMDSLKKPYNLELKNEPGKASLKYIIIDGSNVAISHGLQKFFSCRGIAIAVDYFWKRGHRNITVFVPQWRTRRDPNIKEQHFLTQLQDVGILSLTPARMVLGARIAAHDDRFLLHLADKTGGIIVTNDNFREFVTESYSWREIIQKRLLQYTFAGDIFMVPDDPLGRNGPRLEDFLRSEGCSRDFPLSQITLPTGGLHSSGTSHFMPIPKSCSNRQPKTEVRGGPPSAWPPLEPNFPNVHTGLTIPPQRSSTETIQLREALTKIFPDSEQRQKIDRILAEHPFMRDLNALSAMVLD

Target ORF information:

RefSeq Version	XM_006121241.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis NEDD4 binding protein 1 (N4BP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006121241.2

ORF Insert Sequence:

ATGATTGAAA GAAGAAAACA CCCCTCTGGG GGCAGCATTC AGAAAAAGAA AGAAGCAAAG 
GAAGCTTTTC TATCTAAGCA GGAAGGTGCT CTCCTGAGAC ACACAGACAC AAATGTTCAC 
AGTGAGCCTT CTGGCCCCAG TGAGGATGTG AGTGGTGAGG AGATTCCTGA TCTTCCAGTT 
AGTCAGAGTG CAGGTGACCT GGCACTTACT GCAGCATCCA TATCTCCATC TCAAATGGAT 
GTAACCATGC ACATTCCTGA AGAAAAGTGT AAATCAGAGA ACAGAAAAGA GTGTGAACCT 
AGTGAAAATG TCCCTACAAT GGTGACTGTC AGAGAGAATT TTCTAGAATT TATTGACATT 
GATGATACTA CAGGAGCTGG TATGACAAAT GTGCTTCTTA AAAAGCTGAA AGATATGGGA 
ATTGCGATAG CTGACATGAG AGGGCAGGTC TATTATAATG GTGCCAACAT GAGAGGAAAG 
AACAGAGAAG TGCAGAAACA GATTCAAGAG TTAAACCCTC AAGCTTTTTT TGTCCCATGC 
TGTTCTCATT CATTGAACTT GGTGGAATAT ATTAAGGGAC TTTGTGAACC TGAATTAGAA 
GAAAAGGAAT ATTATCCAAA GGACATGCAC TGCATTTTCG TTGGCGCACA GAGCCTGTTT 
CTTAACTGTC TTATTCAGGA TACCTGTGCA GATATAACAG TGCAGGAAAT AGGATTGCTT 
ATTATTAAGG GTGGTGCAGA AGCTGTTGTA ATGGCTAGAA GTCATATTCA GCAATTTGTG 
AAACTCTTTA AAAACAATGA AAGCTTATTA AGTGACAAGG AATCAAATGT AAAAAAACAA 
TTTAAACAAT TTGTGGAAGC ACATGCAGAT AAATATACAA TGGACTTGTT GATCTTGCCT 
AGCTCACTAA AAAGAGAACT TCTGAATCTT ACACAAAGTG ATTGTTGTGA AGCAGATGGC 
AATATTATAG ATCTTACTAC AGATTTGGTA AGCACATCTG AATTTTCACA GAACAAAACC 
CCAAAAATAA CGATGAGTGG AGATGGAAGA ACTGTTGGGG AGGAAGCAAG AAATAAAGCT 
GGAACCCCTG TAACAGAGCT TACAAAACAA ATGGATACAG TGTTTTCCAA TGCACCAGGA 
AGAGGTTTTA TTCCTATAAG TGGTGTGACT CCACTTGAGA CATCTCTTTC CAAAGAGAGA 
CAGTCATGTA AAAGAAGGTG TTCTGATGCT GAGGAAAGGC TTCCTAAGAA ACAGTTTTCA 
CTAGAAAGTG ATCAGGAGGG CAGGCCTACA CAATGTAGTG AAGATCCAGA TGGGAGAGCA 
GTTGTTGATC TGACTTCTGA TAATTGCAGT GAATTGGATG ATCCACGTTT CTCTATTAAA 
GACTGCGAAG ACATTAGTGA GGAAATGGAA TACAAGATTC TTGTAAACTT TTTCAGGACC 
ATGGGATATT CCCAAAAAAT TGTAGAGAAA GTTATTAGTG AACTGGGACA ATCAGTGGAA 
CCATTAATGC TCCTGGAAGC AATAGAAAAG GAAAGTACAA AGTTTCAGAA GGAAAAAGAA 
CAGTCACAGC AAATATCTAG ATCTGTAAGT GTGCATTCAG GTGGTAATGC AGATCTTTCT 
CAGCCTCTGC AATATAGAGA TTCTGTCAGT AAAAATCAAC TTAAACCAGG TCATACTTTA 
AAGGAGATAA AAAATGTAAA ACAAAGAGAA AATTCTCCAT GTGCACGTCT TCTGCCAGAA 
GACAAAATGC ATGCGTCAAG TTATAAAAAT AAAATTGCTG TTCCTTGTGG TAAAAATTCA 
GCAGAGCATT GTAAACAAGT ACACTTGAAT TCTTTTGATC AAAGTCCCAG GACAAATGAT 
ATAGAGACTG ATGGTCTTGT ACCTTCTTCT AACATTTCAC AAGAATTCAT GAAAAATGGA 
GCACTAAAAA ATGTTGACTT TGTTGCAAGA GGGAGCTCAG ACAGAGCATG TGAATCAGTT 
CAGACTGAAA CTGTAAACCA ACAAAAATCT ACACAGCTCT CGAATACACA GAATAGTCAA 
CCTGATCAAA TGGCGAGACC AGGACATTGC AATCCTTCTC AGCTTAGATT CCTCCACCAA 
CCCTCTTTTG ATATCCACAA ACCTGTTGAA TGTACTTTTC CAAACTCTGT ATTTCCATCC 
CATCCACATG CTCCAAATCT AGAGAGGAAG CCAGCTGGAC CATACAATCA TCATACAGAT 
GCCTCAGTTA CTGGGATTCA AAGATTTATG GATTCTCTGA AAAAGCCATA CAATTTGGAA 
TTGAAAAATG AACCTGGGAA AGCATCTTTA AAATACATCA TTATAGATGG GAGCAACGTT 
GCAATTTCTC ATGGTCTACA GAAGTTCTTC TCCTGTCGAG GAATTGCAAT AGCTGTTGAC 
TATTTCTGGA AACGTGGCCA CAGAAACATT ACGGTGTTTG TTCCACAGTG GAGAACAAGA 
CGTGATCCTA ACATCAAAGA GCAGCATTTC TTAACACAGC TCCAGGATGT TGGAATATTA 
TCTCTAACTC CTGCAAGGAT GGTATTGGGA GCAAGAATTG CTGCTCATGA TGACAGATTT 
TTGCTGCATC TTGCTGATAA AACTGGAGGT ATTATTGTTA CCAATGATAA TTTCAGAGAA 
TTTGTGACGG AATCGTATTC TTGGAGAGAG ATTATTCAAA AAAGGTTGCT CCAATATACA 
TTTGCTGGGG ACATATTCAT GGTTCCTGAT GATCCTTTGG GAAGAAATGG ACCTAGATTA 
GAGGACTTCC TTCGGAGTGA AGGCTGTTCT AGAGATTTTC CATTATCACA AATAACTCTA 
CCAACTGGGG GACTGCATTC TTCTGGGACC TCACACTTCA TGCCAATACC CAAGTCATGC 
AGTAATAGAC AACCTAAGAC TGAAGTACGT GGCGGTCCTC CATCTGCCTG GCCTCCGTTG 
GAACCAAACT TTCCAAATGT ACACACTGGT CTTACAATTC CCCCACAAAG ATCTTCTACA 
GAAACAATAC AGCTAAGGGA AGCGTTGACA AAAATATTTC CCGATTCTGA GCAGAGACAG 
AAGATTGATC GAATACTAGC TGAACATCCA TTCATGAGAG ACCTTAATGC ACTGTCTGCC 
ATGGTACTTG ATTAA