USP25 cDNA ORF clone, Parus major(Great Tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP25
Entrez Gene ID	107208321
Full Name	ubiquitin specific peptidase 25
Gene Type	protein-coding
Organism	Parus major(Great Tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015636628.1	XP_015492114.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_015636638.1	XP_015492124.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2

Pathways from BioSystems
Gene Ontology

The following USP25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa157885	XM_015636628.1 Latest version!	Parus major ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPa157886	XM_015636638.1 Latest version!	Parus major ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP25 ( XM_015636628.1 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015492114.1 Parus major ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa157885
Clone ID Related Accession (Same CDS sequence)	XM_015636628.1
Accession Version	XM_015636628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAAC CCATCAGCTT CTATGAAAAG CACCAGCAAA CATTTTTAAA TCAGCTGAGG 
GAGATCACTG GTATCAATGA CATCCAGGTA CTACAGCAGG CACTGAAGGA CAGCAATGGT 
AATCTGGAGT TGGCTGTGGC TTTTCTCACT GCAAAGAATG CCAAGGTTCC CCAGCTAGAG 
GAGGCAACTT ACTACCAAAC AGCCCTTCCT GGGAATGACA GATACATCAG TGTGGGCAGC 
CAGGCAGACA CCAATGTGAT TGATCTCACT GGAGATGATA AGGATGATCT TCAGAGGGCA 
ATTGCCTTGA GTTTGGAGGA ATCAAACAGG GCATTCAGGG AGACTGGAAT AACAGATGAG 
GAACAAGCCA TTAGCAGAGT TCTAGAAGCC AGTATTGCAG AAAATAAAGC GAGTTTAAAG 
AGGACACATC CAGAGGTATG GAGTGACTCT CCAAACCCTT ATGATCGAAA GCGGCAAGAC 
AACTGCCCAG TAGGATTAAA GAACGTTGGC AACACGTGCT GGTTTAGTGC TGTCATTCAG 
TCATTATTTA ACCTGCTGGA GTTTCGAAGA CTAGTTTTGA ATTTCAATCC TCCTGCAAAC 
GCTCAAGATT TGCCCCGAAA CCAAAAGGAA CATAGGAATC TGCCTTTCAT GCGTGAATTG 
AGGTACCTGT TTGCACTTCT AGTTGGTTCA AAGAGAAAAT ATGTTGATCC ATCAAGAGCA 
GTTGAAATCC TTAAAGATGC TTTTAAATCA AATGATTCCC AACAGCAAGA TGTTAGTGAA 
TTTACACACA AATTATTAGA TTGGCTAGAA GATGCCTTTC AAATTAAAGC TGAAGAAGAG 
AGGGATGGAG AGAAACCAAA GAACCCAATG GTGGAGTTGT TCTATGGACG ATTTCTAGCA 
GTAGGTGTAC TTGAAGGTAA AAAATTTGAA AACACAGAAA TGTTTGGCCA GTATCCACTA 
CAGGTTAATG GCTTTAAAGA TCTGCATGAG TGCCTAGAAG CTGCAATGAT TGAAGGGGAA 
ATTGAATCTC TCCATTCAGA AAACTCTGCA AAGTCTGGTC AGGAGCACTG GTTCACAGAG 
CTTCCTCCTG TCTTAACTTT TGAGCTATCA AGATTTGAGT TTAATCAGGC TTTGGGAAGA 
CCAGAGAAGA TACACAACAA ATTAGAGTTT CCTCCAATTT TGTATCTAGA CAGGTATATG 
CACAAAAACA GAGAAATAAC AAGAATTAAA CGGGATGAAA TCAAGAGACT CAAAGAGTAC 
CTCACAGTTT TACAACAGAG ATTAGAACGA TATTTAAGCT ATGGTTCTGG TCCTAAACGA 
TTCCCCTTGG TAGATGTCCT GCAGTATGCC CTGGAGTTCG CCTCCAGCAA GCCAGTGTGC 
ACGTCCCCTG TGGATGACCT GGGTGCCAGT GCCTCTGCCA GCGGCACACT GCCGCCACAG 
ATACCCCCCA GCACAACAGA GCAGCAGGGG CCTTCATCAT CAGATGTCCC AAGCACATCT 
CCTGTACAAA GATCAGTAAT CCATAAACCA TTCACACAAT CACGGATTCC CCCTGACCTG 
CCAATGCATC CGGCACCAAG GCACATCACG GAGGAGGAGC TGTCTGTCCT GGAGGGCTGT 
TTGCATCGCT GGAGGACTGA AGTAGAGAAT GACACAAGAG ATTTGCAAGA AAGTATATCT 
AGAATACATC GGACAATTGA ATTGATGTAC TCTGACAAAA CAATGGTCCA AGTTCCTTAT 
AGGTTGCATG CTGTCCTAGT TCATGAGGGT CAAGCGAATG CAGGCCACTA CTGGGCATAT 
ATTTATGACC ACCACCAGAA CAGATGGATG AAATATAATG ATATTTCTGT GACAAAGTCA 
ACTTGGGAGG AGTTGGAACG GGACTCTTTT GGTGGTTACA GGAATGCCAG TGCATATTGT 
TTAATGTATA TCAATGACAA GGAGCAGTAC TTGATACAAG AGGAGTTCAA CAAAGAAACA 
GGGCAAATCC TTGTTGGAAT GGACACACTG CCTTCTGATC TAAGGGACTA TGTCAAAGAA 
GACAATAAAC GTTTTGAAAA AGAACTTGAA GAGTGGGATG CAGAACTTGC TCAGAAAACA 
CAGCAGGAGA AATTGTTATC TCAGATACCT AGGGCACCAG CACCCTCACC TGCTCCAGTT 
CAAGCAGGAG AACCAGAATA TTTAGAGCAG CCATCAAGAA CTGATATTTC AAAGCACTTA 
AAAGAAGATT CTGTACAAGC AATCAATAAA GCTTTAGCTG AACAAGAAGA TAGAGGTCCA 
GAAGCTATAA TGGATACGAG CTCTGCTAAT GCCCCAGCTC AAGCAGCTCC TACCCAGCGA 
GTGGTAGAGG TGGCGATCCC TGATGTAGGG ACTTTTGTGA TTGAGTCAGA GGAGGGGGGT 
TATGACGATG AGGTCATGCT GACCCCGAAC ATGCAAGGTA TTATCATGGC TATAGGTAAA 
GCCAGGAATG TTTATGACAG GTGTGGGCCA GAAGCAGGGT TCTTTAAGGC AATTAAATTG 
GAATACACAC GTCTGCTAAA ATTAGCCCAG GAAGATCCAC CACCTGAATG TGATTATCGT 
TTGCGTCATG CAATTGTTTA CTTCATCCAA AACCAGGCAC CAAAGAAGAT AATTGAGAGA 
ACATTACTGG AACAGTTTGG AGATCACAAT CTCAGCTTTG ATGAAAGGTG CCGTAACATA 
ATGAAGGTTG CTCAAGCCAA ACTTGAAATG ATAAAGCCAG ATGAAGTAAA CATGGAGGAA 
TATGAGAGAT GGCATCAAGA CTATAGACAT TTCAGGGAAA CAACCATGTT TCTCATGGTA 
GGATTGGAGT TTTTCCAAAA AAAGAGTTAC ATGGAAGCCT TGCTCTACCT TATCTATGCT 
TACCAGAATA ACAAAGAACT CTTGTCCAAA GGCCCGTACA GAGGGCACGA TGAAGAGCTG 
ATATCTCACT ACAGACGAGA GTGTTTGCTA AAACTAAACG AACATGCTGC AGCACTGTTT 
GAATCAGGAG ATGATCAGGA AGTAAATAAT GGCCTGATCA TAATGAACGA GCTTATTGTC 
CCATGTTTGC CATTACTACT GGTGGATGAA ATGGAAGAGA AAGATATTGT TGCTGTGGAA 
GATATGAGAA ACCGTTGGTG TTCCTATCTT GGACAAGAAA TGGAACCCAA CTTGCAAGAA 
AAACTGACAG ATTTCCTGCC AAAACTGCTA GATTGTTCTA CAGAGATTAA AGGTTTCAAT 
GACCCGCCAA AGTTACCTTC CTACTCCACA CATGAACTGT GTGAAAGATA TGCCCGAATC 
ATGTTGTCAC TCAGTCGAAC TCCAGCCGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015492114.1
CDS	16..3354
Translation	MIKPISFYEKHQQTFLNQLREITGINDIQVLQQALKDSNGNLELAVAFLTAKNAKVPQLEEATYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHPEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNFNPPANAQDLPRNQKEHRNLPFMRELRYLFALLVGSKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQIKAEEERDGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPPILYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDLGASASASGTLPPQIPPSTTEQQGPSSSDVPSTSPVQRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIELMYSDKTMVQVPYRLHAVLVHEGQANAGHYWAYIYDHHQNRWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQYLIQEEFNKETGQILVGMDTLPSDLRDYVKEDNKRFEKELEEWDAELAQKTQQEKLLSQIPRAPAPSPAPVQAGEPEYLEQPSRTDISKHLKEDSVQAINKALAEQEDRGPEAIMDTSSANAPAQAAPTQRVVEVAIPDVGTFVIESEEGGYDDEVMLTPNMQGIIMAIGKARNVYDRCGPEAGFFKAIKLEYTRLLKLAQEDPPPECDYRLRHAIVYFIQNQAPKKIIERTLLEQFGDHNLSFDERCRNIMKVAQAKLEMIKPDEVNMEEYERWHQDYRHFRETTMFLMVGLEFFQKKSYMEALLYLIYAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAALFESGDDQEVNNGLIIMNELIVPCLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNDPPKLPSYSTHELCERYARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_015636628.1
Organism	Parus major(Great Tit)
Definition	Parus major ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015636628.1

ORF Insert Sequence:

ATGATAAAAC CCATCAGCTT CTATGAAAAG CACCAGCAAA CATTTTTAAA TCAGCTGAGG 
GAGATCACTG GTATCAATGA CATCCAGGTA CTACAGCAGG CACTGAAGGA CAGCAATGGT 
AATCTGGAGT TGGCTGTGGC TTTTCTCACT GCAAAGAATG CCAAGGTTCC CCAGCTAGAG 
GAGGCAACTT ACTACCAAAC AGCCCTTCCT GGGAATGACA GATACATCAG TGTGGGCAGC 
CAGGCAGACA CCAATGTGAT TGATCTCACT GGAGATGATA AGGATGATCT TCAGAGGGCA 
ATTGCCTTGA GTTTGGAGGA ATCAAACAGG GCATTCAGGG AGACTGGAAT AACAGATGAG 
GAACAAGCCA TTAGCAGAGT TCTAGAAGCC AGTATTGCAG AAAATAAAGC GAGTTTAAAG 
AGGACACATC CAGAGGTATG GAGTGACTCT CCAAACCCTT ATGATCGAAA GCGGCAAGAC 
AACTGCCCAG TAGGATTAAA GAACGTTGGC AACACGTGCT GGTTTAGTGC TGTCATTCAG 
TCATTATTTA ACCTGCTGGA GTTTCGAAGA CTAGTTTTGA ATTTCAATCC TCCTGCAAAC 
GCTCAAGATT TGCCCCGAAA CCAAAAGGAA CATAGGAATC TGCCTTTCAT GCGTGAATTG 
AGGTACCTGT TTGCACTTCT AGTTGGTTCA AAGAGAAAAT ATGTTGATCC ATCAAGAGCA 
GTTGAAATCC TTAAAGATGC TTTTAAATCA AATGATTCCC AACAGCAAGA TGTTAGTGAA 
TTTACACACA AATTATTAGA TTGGCTAGAA GATGCCTTTC AAATTAAAGC TGAAGAAGAG 
AGGGATGGAG AGAAACCAAA GAACCCAATG GTGGAGTTGT TCTATGGACG ATTTCTAGCA 
GTAGGTGTAC TTGAAGGTAA AAAATTTGAA AACACAGAAA TGTTTGGCCA GTATCCACTA 
CAGGTTAATG GCTTTAAAGA TCTGCATGAG TGCCTAGAAG CTGCAATGAT TGAAGGGGAA 
ATTGAATCTC TCCATTCAGA AAACTCTGCA AAGTCTGGTC AGGAGCACTG GTTCACAGAG 
CTTCCTCCTG TCTTAACTTT TGAGCTATCA AGATTTGAGT TTAATCAGGC TTTGGGAAGA 
CCAGAGAAGA TACACAACAA ATTAGAGTTT CCTCCAATTT TGTATCTAGA CAGGTATATG 
CACAAAAACA GAGAAATAAC AAGAATTAAA CGGGATGAAA TCAAGAGACT CAAAGAGTAC 
CTCACAGTTT TACAACAGAG ATTAGAACGA TATTTAAGCT ATGGTTCTGG TCCTAAACGA 
TTCCCCTTGG TAGATGTCCT GCAGTATGCC CTGGAGTTCG CCTCCAGCAA GCCAGTGTGC 
ACGTCCCCTG TGGATGACCT GGGTGCCAGT GCCTCTGCCA GCGGCACACT GCCGCCACAG 
ATACCCCCCA GCACAACAGA GCAGCAGGGG CCTTCATCAT CAGATGTCCC AAGCACATCT 
CCTGTACAAA GATCAGTAAT CCATAAACCA TTCACACAAT CACGGATTCC CCCTGACCTG 
CCAATGCATC CGGCACCAAG GCACATCACG GAGGAGGAGC TGTCTGTCCT GGAGGGCTGT 
TTGCATCGCT GGAGGACTGA AGTAGAGAAT GACACAAGAG ATTTGCAAGA AAGTATATCT 
AGAATACATC GGACAATTGA ATTGATGTAC TCTGACAAAA CAATGGTCCA AGTTCCTTAT 
AGGTTGCATG CTGTCCTAGT TCATGAGGGT CAAGCGAATG CAGGCCACTA CTGGGCATAT 
ATTTATGACC ACCACCAGAA CAGATGGATG AAATATAATG ATATTTCTGT GACAAAGTCA 
ACTTGGGAGG AGTTGGAACG GGACTCTTTT GGTGGTTACA GGAATGCCAG TGCATATTGT 
TTAATGTATA TCAATGACAA GGAGCAGTAC TTGATACAAG AGGAGTTCAA CAAAGAAACA 
GGGCAAATCC TTGTTGGAAT GGACACACTG CCTTCTGATC TAAGGGACTA TGTCAAAGAA 
GACAATAAAC GTTTTGAAAA AGAACTTGAA GAGTGGGATG CAGAACTTGC TCAGAAAACA 
CAGCAGGAGA AATTGTTATC TCAGATACCT AGGGCACCAG CACCCTCACC TGCTCCAGTT 
CAAGCAGGAG AACCAGAATA TTTAGAGCAG CCATCAAGAA CTGATATTTC AAAGCACTTA 
AAAGAAGATT CTGTACAAGC AATCAATAAA GCTTTAGCTG AACAAGAAGA TAGAGGTCCA 
GAAGCTATAA TGGATACGAG CTCTGCTAAT GCCCCAGCTC AAGCAGCTCC TACCCAGCGA 
GTGGTAGAGG TGGCGATCCC TGATGTAGGG ACTTTTGTGA TTGAGTCAGA GGAGGGGGGT 
TATGACGATG AGGTCATGCT GACCCCGAAC ATGCAAGGTA TTATCATGGC TATAGGTAAA 
GCCAGGAATG TTTATGACAG GTGTGGGCCA GAAGCAGGGT TCTTTAAGGC AATTAAATTG 
GAATACACAC GTCTGCTAAA ATTAGCCCAG GAAGATCCAC CACCTGAATG TGATTATCGT 
TTGCGTCATG CAATTGTTTA CTTCATCCAA AACCAGGCAC CAAAGAAGAT AATTGAGAGA 
ACATTACTGG AACAGTTTGG AGATCACAAT CTCAGCTTTG ATGAAAGGTG CCGTAACATA 
ATGAAGGTTG CTCAAGCCAA ACTTGAAATG ATAAAGCCAG ATGAAGTAAA CATGGAGGAA 
TATGAGAGAT GGCATCAAGA CTATAGACAT TTCAGGGAAA CAACCATGTT TCTCATGGTA 
GGATTGGAGT TTTTCCAAAA AAAGAGTTAC ATGGAAGCCT TGCTCTACCT TATCTATGCT 
TACCAGAATA ACAAAGAACT CTTGTCCAAA GGCCCGTACA GAGGGCACGA TGAAGAGCTG 
ATATCTCACT ACAGACGAGA GTGTTTGCTA AAACTAAACG AACATGCTGC AGCACTGTTT 
GAATCAGGAG ATGATCAGGA AGTAAATAAT GGCCTGATCA TAATGAACGA GCTTATTGTC 
CCATGTTTGC CATTACTACT GGTGGATGAA ATGGAAGAGA AAGATATTGT TGCTGTGGAA 
GATATGAGAA ACCGTTGGTG TTCCTATCTT GGACAAGAAA TGGAACCCAA CTTGCAAGAA 
AAACTGACAG ATTTCCTGCC AAAACTGCTA GATTGTTCTA CAGAGATTAA AGGTTTCAAT 
GACCCGCCAA AGTTACCTTC CTACTCCACA CATGAACTGT GTGAAAGATA TGCCCGAATC 
ATGTTGTCAC TCAGTCGAAC TCCAGCCGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_015636638.1 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015492124.1 Parus major ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa157886
Clone ID Related Accession (Same CDS sequence)	XM_015636638.1
Accession Version	XM_015636638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAAC CCATCAGCTT CTATGAAAAG CACCAGCAAA CATTTTTAAA TCAGCTGAGG 
GAGATCACTG GTATCAATGA CATCCAGGTA CTACAGCAGG CACTGAAGGA CAGCAATGGT 
AATCTGGAGT TGGCTGTGGC TTTTCTCACT GCAAAGAATG CCAAGGTTCC CCAGCTAGAG 
GAGGCAACTT ACTACCAAAC AGCCCTTCCT GGGAATGACA GATACATCAG TGTGGGCAGC 
CAGGCAGACA CCAATGTGAT TGATCTCACT GGAGATGATA AGGATGATCT TCAGAGGGCA 
ATTGCCTTGA GTTTGGAGGA ATCAAACAGG GCATTCAGGG AGACTGGAAT AACAGATGAG 
GAACAAGCCA TTAGCAGAGT TCTAGAAGCC AGTATTGCAG AAAATAAAGC GAGTTTAAAG 
AGGACACATC CAGAGGTATG GAGTGACTCT CCAAACCCTT ATGATCGAAA GCGGCAAGAC 
AACTGCCCAG TAGGATTAAA GAACGTTGGC AACACGTGCT GGTTTAGTGC TGTCATTCAG 
TCATTATTTA ACCTGCTGGA GTTTCGAAGA CTAGTTTTGA ATTTCAATCC TCCTGCAAAC 
GCTCAAGATT TGCCCCGAAA CCAAAAGGAA CATAGGAATC TGCCTTTCAT GCGTGAATTG 
AGGTACCTGT TTGCACTTCT AGTTGGTTCA AAGAGAAAAT ATGTTGATCC ATCAAGAGCA 
GTTGAAATCC TTAAAGATGC TTTTAAATCA AATGATTCCC AACAGCAAGA TGTTAGTGAA 
TTTACACACA AATTATTAGA TTGGCTAGAA GATGCCTTTC AAATTAAAGC TGAAGAAGAG 
AGGGATGGAG AGAAACCAAA GAACCCAATG GTGGAGTTGT TCTATGGACG ATTTCTAGCA 
GTAGGTGTAC TTGAAGGTAA AAAATTTGAA AACACAGAAA TGTTTGGCCA GTATCCACTA 
CAGGTTAATG GCTTTAAAGA TCTGCATGAG TGCCTAGAAG CTGCAATGAT TGAAGGGGAA 
ATTGAATCTC TCCATTCAGA AAACTCTGCA AAGTCTGGTC AGGAGCACTG GTTCACAGAG 
CTTCCTCCTG TCTTAACTTT TGAGCTATCA AGATTTGAGT TTAATCAGGC TTTGGGAAGA 
CCAGAGAAGA TACACAACAA ATTAGAGTTT CCTCCAATTT TGTATCTAGA CAGGTATATG 
CACAAAAACA GAGAAATAAC AAGAATTAAA CGGGATGAAA TCAAGAGACT CAAAGAGTAC 
CTCACAGTTT TACAACAGAG ATTAGAACGA TATTTAAGCT ATGGTTCTGG TCCTAAACGA 
TTCCCCTTGG TAGATGTCCT GCAGTATGCC CTGGAGTTCG CCTCCAGCAA GCCAGTGTGC 
ACGTCCCCTG TGGATGACCT GGGTGCCAGT GCCTCTGCCA GCGGCACACT GCCGCCACAG 
ATACCCCCCA GCACAACAGA GCAGCAGGGG CCTTCATCAT CAGATGTCCC AAGCACATCT 
CCTGTACAAA GATCAGTAAT CCATAAACCA TTCACACAAT CACGGATTCC CCCTGACCTG 
CCAATGCATC CGGCACCAAG GCACATCACG GAGGAGGAGC TGTCTGTCCT GGAGGGCTGT 
TTGCATCGCT GGAGGACTGA AGTAGAGAAT GACACAAGAG ATTTGCAAGA AAGTATATCT 
AGAATACATC GGACAATTGA ATTGATGTAC TCTGACAAAA CAATGGTCCA AGTTCCTTAT 
AGGTTGCATG CTGTCCTAGT TCATGAGGGT CAAGCGAATG CAGGCCACTA CTGGGCATAT 
ATTTATGACC ACCACCAGAA CAGATGGATG AAATATAATG ATATTTCTGT GACAAAGTCA 
ACTTGGGAGG AGTTGGAACG GGACTCTTTT GGTGGTTACA GGAATGCCAG TGCATATTGT 
TTAATGTATA TCAATGACAA GGAGCAGTAC TTGATACAAG AGGAGTTCAA CAAAGAAACA 
GGGCAAATCC TTGTTGGAAT GGACACACTG CCTTCTGATC TAAGGGACTA TGTCAAAGAA 
GACAATAAAC GTTTTGAAAA AGAACTTGAA GAGTGGGATG CAGAACTTGC TCAGAAAACA 
CAGCAGGAGA AATTGTTATC TCAGATACCT AGGGCACCAG CACCCTCACC TGCTCCAGTT 
CAAGCAGGAG AACCAGAATA TTTAGAGCAG CCATCAAGAA CTGATATTTC AAAGCACTTA 
AAAGAAGATT CTGTACAAGC AATCAATAAA GCTTTAGCTG AACAAGAAGA TAGAGGTCCA 
GAAGCTATAA TGGATACGGC AATTAAATTG GAATACACAC GTCTGCTAAA ATTAGCCCAG 
GAAGATCCAC CACCTGAATG TGATTATCGT TTGCGTCATG CAATTGTTTA CTTCATCCAA 
AACCAGGCAC CAAAGAAGAT AATTGAGAGA ACATTACTGG AACAGTTTGG AGATCACAAT 
CTCAGCTTTG ATGAAAGGTG CCGTAACATA ATGAAGGTTG CTCAAGCCAA ACTTGAAATG 
ATAAAGCCAG ATGAAGTAAA CATGGAGGAA TATGAGAGAT GGCATCAAGA CTATAGACAT 
TTCAGGGAAA CAACCATGTT TCTCATGGTA GGATTGGAGT TTTTCCAAAA AAAGAGTTAC 
ATGGAAGCCT TGCTCTACCT TATCTATGCT TACCAGAATA ACAAAGAACT CTTGTCCAAA 
GGCCCGTACA GAGGGCACGA TGAAGAGCTG ATATCTCACT ACAGACGAGA GTGTTTGCTA 
AAACTAAACG AACATGCTGC AGCACTGTTT GAATCAGGAG ATGATCAGGA AGTAAATAAT 
GGCCTGATCA TAATGAACGA GCTTATTGTC CCATGTTTGC CATTACTACT GGTGGATGAA 
ATGGAAGAGA AAGATATTGT TGCTGTGGAA GATATGAGAA ACCGTTGGTG TTCCTATCTT 
GGACAAGAAA TGGAACCCAA CTTGCAAGAA AAACTGACAG ATTTCCTGCC AAAACTGCTA 
GATTGTTCTA CAGAGATTAA AGGTTTCAAT GACCCGCCAA AGTTACCTTC CTACTCCACA 
CATGAACTGT GTGAAAGATA TGCCCGAATC ATGTTGTCAC TCAGTCGAAC TCCAGCCGAT 
GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015492124.1
CDS	16..3144
Translation	MIKPISFYEKHQQTFLNQLREITGINDIQVLQQALKDSNGNLELAVAFLTAKNAKVPQLEEATYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHPEVWSDSPNPYDRKRQDNCPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNFNPPANAQDLPRNQKEHRNLPFMRELRYLFALLVGSKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQIKAEEERDGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSAKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPPILYLDRYMHKNREITRIKRDEIKRLKEYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDLGASASASGTLPPQIPPSTTEQQGPSSSDVPSTSPVQRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLEGCLHRWRTEVENDTRDLQESISRIHRTIELMYSDKTMVQVPYRLHAVLVHEGQANAGHYWAYIYDHHQNRWMKYNDISVTKSTWEELERDSFGGYRNASAYCLMYINDKEQYLIQEEFNKETGQILVGMDTLPSDLRDYVKEDNKRFEKELEEWDAELAQKTQQEKLLSQIPRAPAPSPAPVQAGEPEYLEQPSRTDISKHLKEDSVQAINKALAEQEDRGPEAIMDTAIKLEYTRLLKLAQEDPPPECDYRLRHAIVYFIQNQAPKKIIERTLLEQFGDHNLSFDERCRNIMKVAQAKLEMIKPDEVNMEEYERWHQDYRHFRETTMFLMVGLEFFQKKSYMEALLYLIYAYQNNKELLSKGPYRGHDEELISHYRRECLLKLNEHAAALFESGDDQEVNNGLIIMNELIVPCLPLLLVDEMEEKDIVAVEDMRNRWCSYLGQEMEPNLQEKLTDFLPKLLDCSTEIKGFNDPPKLPSYSTHELCERYARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_015636638.1
Organism	Parus major(Great Tit)
Definition	Parus major ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015636638.1

ORF Insert Sequence:

ATGATAAAAC CCATCAGCTT CTATGAAAAG CACCAGCAAA CATTTTTAAA TCAGCTGAGG 
GAGATCACTG GTATCAATGA CATCCAGGTA CTACAGCAGG CACTGAAGGA CAGCAATGGT 
AATCTGGAGT TGGCTGTGGC TTTTCTCACT GCAAAGAATG CCAAGGTTCC CCAGCTAGAG 
GAGGCAACTT ACTACCAAAC AGCCCTTCCT GGGAATGACA GATACATCAG TGTGGGCAGC 
CAGGCAGACA CCAATGTGAT TGATCTCACT GGAGATGATA AGGATGATCT TCAGAGGGCA 
ATTGCCTTGA GTTTGGAGGA ATCAAACAGG GCATTCAGGG AGACTGGAAT AACAGATGAG 
GAACAAGCCA TTAGCAGAGT TCTAGAAGCC AGTATTGCAG AAAATAAAGC GAGTTTAAAG 
AGGACACATC CAGAGGTATG GAGTGACTCT CCAAACCCTT ATGATCGAAA GCGGCAAGAC 
AACTGCCCAG TAGGATTAAA GAACGTTGGC AACACGTGCT GGTTTAGTGC TGTCATTCAG 
TCATTATTTA ACCTGCTGGA GTTTCGAAGA CTAGTTTTGA ATTTCAATCC TCCTGCAAAC 
GCTCAAGATT TGCCCCGAAA CCAAAAGGAA CATAGGAATC TGCCTTTCAT GCGTGAATTG 
AGGTACCTGT TTGCACTTCT AGTTGGTTCA AAGAGAAAAT ATGTTGATCC ATCAAGAGCA 
GTTGAAATCC TTAAAGATGC TTTTAAATCA AATGATTCCC AACAGCAAGA TGTTAGTGAA 
TTTACACACA AATTATTAGA TTGGCTAGAA GATGCCTTTC AAATTAAAGC TGAAGAAGAG 
AGGGATGGAG AGAAACCAAA GAACCCAATG GTGGAGTTGT TCTATGGACG ATTTCTAGCA 
GTAGGTGTAC TTGAAGGTAA AAAATTTGAA AACACAGAAA TGTTTGGCCA GTATCCACTA 
CAGGTTAATG GCTTTAAAGA TCTGCATGAG TGCCTAGAAG CTGCAATGAT TGAAGGGGAA 
ATTGAATCTC TCCATTCAGA AAACTCTGCA AAGTCTGGTC AGGAGCACTG GTTCACAGAG 
CTTCCTCCTG TCTTAACTTT TGAGCTATCA AGATTTGAGT TTAATCAGGC TTTGGGAAGA 
CCAGAGAAGA TACACAACAA ATTAGAGTTT CCTCCAATTT TGTATCTAGA CAGGTATATG 
CACAAAAACA GAGAAATAAC AAGAATTAAA CGGGATGAAA TCAAGAGACT CAAAGAGTAC 
CTCACAGTTT TACAACAGAG ATTAGAACGA TATTTAAGCT ATGGTTCTGG TCCTAAACGA 
TTCCCCTTGG TAGATGTCCT GCAGTATGCC CTGGAGTTCG CCTCCAGCAA GCCAGTGTGC 
ACGTCCCCTG TGGATGACCT GGGTGCCAGT GCCTCTGCCA GCGGCACACT GCCGCCACAG 
ATACCCCCCA GCACAACAGA GCAGCAGGGG CCTTCATCAT CAGATGTCCC AAGCACATCT 
CCTGTACAAA GATCAGTAAT CCATAAACCA TTCACACAAT CACGGATTCC CCCTGACCTG 
CCAATGCATC CGGCACCAAG GCACATCACG GAGGAGGAGC TGTCTGTCCT GGAGGGCTGT 
TTGCATCGCT GGAGGACTGA AGTAGAGAAT GACACAAGAG ATTTGCAAGA AAGTATATCT 
AGAATACATC GGACAATTGA ATTGATGTAC TCTGACAAAA CAATGGTCCA AGTTCCTTAT 
AGGTTGCATG CTGTCCTAGT TCATGAGGGT CAAGCGAATG CAGGCCACTA CTGGGCATAT 
ATTTATGACC ACCACCAGAA CAGATGGATG AAATATAATG ATATTTCTGT GACAAAGTCA 
ACTTGGGAGG AGTTGGAACG GGACTCTTTT GGTGGTTACA GGAATGCCAG TGCATATTGT 
TTAATGTATA TCAATGACAA GGAGCAGTAC TTGATACAAG AGGAGTTCAA CAAAGAAACA 
GGGCAAATCC TTGTTGGAAT GGACACACTG CCTTCTGATC TAAGGGACTA TGTCAAAGAA 
GACAATAAAC GTTTTGAAAA AGAACTTGAA GAGTGGGATG CAGAACTTGC TCAGAAAACA 
CAGCAGGAGA AATTGTTATC TCAGATACCT AGGGCACCAG CACCCTCACC TGCTCCAGTT 
CAAGCAGGAG AACCAGAATA TTTAGAGCAG CCATCAAGAA CTGATATTTC AAAGCACTTA 
AAAGAAGATT CTGTACAAGC AATCAATAAA GCTTTAGCTG AACAAGAAGA TAGAGGTCCA 
GAAGCTATAA TGGATACGGC AATTAAATTG GAATACACAC GTCTGCTAAA ATTAGCCCAG 
GAAGATCCAC CACCTGAATG TGATTATCGT TTGCGTCATG CAATTGTTTA CTTCATCCAA 
AACCAGGCAC CAAAGAAGAT AATTGAGAGA ACATTACTGG AACAGTTTGG AGATCACAAT 
CTCAGCTTTG ATGAAAGGTG CCGTAACATA ATGAAGGTTG CTCAAGCCAA ACTTGAAATG 
ATAAAGCCAG ATGAAGTAAA CATGGAGGAA TATGAGAGAT GGCATCAAGA CTATAGACAT 
TTCAGGGAAA CAACCATGTT TCTCATGGTA GGATTGGAGT TTTTCCAAAA AAAGAGTTAC 
ATGGAAGCCT TGCTCTACCT TATCTATGCT TACCAGAATA ACAAAGAACT CTTGTCCAAA 
GGCCCGTACA GAGGGCACGA TGAAGAGCTG ATATCTCACT ACAGACGAGA GTGTTTGCTA 
AAACTAAACG AACATGCTGC AGCACTGTTT GAATCAGGAG ATGATCAGGA AGTAAATAAT 
GGCCTGATCA TAATGAACGA GCTTATTGTC CCATGTTTGC CATTACTACT GGTGGATGAA 
ATGGAAGAGA AAGATATTGT TGCTGTGGAA GATATGAGAA ACCGTTGGTG TTCCTATCTT 
GGACAAGAAA TGGAACCCAA CTTGCAAGAA AAACTGACAG ATTTCCTGCC AAAACTGCTA 
GATTGTTCTA CAGAGATTAA AGGTTTCAAT GACCCGCCAA AGTTACCTTC CTACTCCACA 
CATGAACTGT GTGAAAGATA TGCCCGAATC ATGTTGTCAC TCAGTCGAAC TCCAGCCGAT 
GGAAGATAA