TNF cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNF
Entrez Gene ID	443540
Full Name	tumor necrosis factor
Synonyms	TNF-alpha
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012100437.2	XP_011955827.1	tumor necrosis factor isoform X1
XM_012100437.3	XP_011955827.2	tumor necrosis factor isoform X1
NM_001024860.1	NP_001020031.1	tumor necrosis factor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Tumor Necrosis Factor-?, Interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in Ossimi sheep with encephalitic listeriosis.
Abdlla OA, Elboshy ME, Reisha EF, Gadlla HA, El-Khodery SA
Cytokine73(2)283-7(2015 Jun)

Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?
Hodges PW, James G, Blomster L, Hall L, Schmid AB, Shu C, Little C, Melrose J
Spine39(13)1010-7(2014 Jun)

Peripheral injection of SB203580 inhibits the inflammatory-dependent synthesis of proinflammatory cytokines in the hypothalamus.
Herman AP, Krawczy?ska A, Bochenek J, Antushevich H, Herman A, Tomaszewska-Zaremba D
BioMed research international2014475152(2014)

Tumor necrosis factor-alpha and acute-phase proteins in early pregnant ewes after challenge with peptidoglycan-polysaccharide.
Dow TL, Rogers-Nieman G, Hol?skov? I, Elsasser TH, Dailey RA
Domestic animal endocrinology39(2)147-54(2010 Aug)

Elevated plasma levels of interleukin 1 beta, tumour necrosis factor alpha and monocyte chemotactic protein 1 are associated with pregnancy toxaemia in ewes.
Yarim GF, Karahan S, Nisbet C
Veterinary research communications31(5)565-73(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis.
Title: Tumor Necrosis Factor-?, Interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in Ossimi sheep with encephalitic listeriosis.

TNF-alpha gene expression in multifidus was greater on both sides in the disc lesion animals than in the muscle of control animals.
Title: Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?

The p38 MAPK inhibitor decreased (P < 0.01) gene expression of TNFa but its effect was not observed at the level of TNFa protein synthesis
Title: Peripheral injection of SB203580 inhibits the inflammatory-dependent synthesis of proinflammatory cytokines in the hypothalamus.

Injection of peptidoglycan-polysaccharide resulted in fever, increased concentrations of TNF-alpha on the day of and the day after challenge, and decreased concentrations of progesterone on days 14 and 21.
Title: Tumor necrosis factor-alpha and acute-phase proteins in early pregnant ewes after challenge with peptidoglycan-polysaccharide.

plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes
Title: Elevated plasma levels of interleukin 1 beta, tumour necrosis factor alpha and monocyte chemotactic protein 1 are associated with pregnancy toxaemia in ewes.

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm21566	NM_001024860.1 Latest version!	Ovis aries tumor necrosis factor (TNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSm21918	XM_012100437.2 Latest version!	Ovis aries tumor necrosis factor (TNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSm71317	XM_012100437.3 Latest version!	Ovis aries tumor necrosis factor (TNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNF ( NM_001024860.1 ) cDNA ORF clone, Ovis aries(sheep) -> NP_001020031.1 Ovis aries tumor necrosis factor (TNF), mRNA.

Price & Availability↑

CloneID	OSm21566
Clone ID Related Accession (Same CDS sequence)	NM_001024860.1
Accession Version	NM_001024860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Ovis aries(sheep)
Product	tumor necrosis factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X56756.1. ##Evidence-Data-START## Transcript exon combination :: X56756.1, X55152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA104495058 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCAACAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TGGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGCAGT CCCCAGCTGG CCCCTCCTTC AACAGGCCTC TGGTTCAGAC ACTCAGGTCA 
TCTTCTCAAG CCTCAAATAA CAAGCCGGTA GCCCACGTTG TAGCCAACAT CAGCGCTCCG 
GGGCAGCTCC GATGGGGGGA CTCGTATGCC AATGCCCTCA TGGCCAACGG CGTGGAGCTG 
AAAGACAACC AGCTGGTGGT GCCCACTGAC GGGCTTTACC TCATCTACTC GCAGGTCCTC 
TTCAGGGGCC ACGGCTGCCC TTCCACCCCC TTGTTCCTCA CCCACACCAT CAGCCGCATT 
GCAGTCTCCT ACCAGACCAA GGTCAACATC CTCTCTGCCA TCAAGAGCCC TTGCCACAGG 
GAGACCCTAG AGGGGGCTGA GGCCAAGCCC TGGTACGAAC CCATCTACCA GGGAGGGGTC 
TTCCAGCTGG AGAAGGGAGA TCGCCTCAGT GCTGAGATCA ACCTGCCGGA ATACCTGGAC 
TATGCCGAGT CTGGGCAGGT CTACTTTGGG ATCATCGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020031.1
CDS	173..877
Translation	MSTKSMIRDVELAEEVLSNKAGGPQGSRSCWCLSLFSFLLVAGATTLFCLLHFGVIGPQREEQSPAGPSFNRPLVQTLRSSSQASNNKPVAHVVANISAPGQLRWGDSYANALMANGVELKDNQLVVPTDGLYLIYSQVLFRGHGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETLEGAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPEYLDYAESGQVYFGIIAL

Target ORF information:

RefSeq Version	NM_001024860.1
Organism	Ovis aries(sheep)
Definition	Ovis aries tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024860.1

ORF Insert Sequence:

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCAACAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TGGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGCAGT CCCCAGCTGG CCCCTCCTTC AACAGGCCTC TGGTTCAGAC ACTCAGGTCA 
TCTTCTCAAG CCTCAAATAA CAAGCCGGTA GCCCACGTTG TAGCCAACAT CAGCGCTCCG 
GGGCAGCTCC GATGGGGGGA CTCGTATGCC AATGCCCTCA TGGCCAACGG CGTGGAGCTG 
AAAGACAACC AGCTGGTGGT GCCCACTGAC GGGCTTTACC TCATCTACTC GCAGGTCCTC 
TTCAGGGGCC ACGGCTGCCC TTCCACCCCC TTGTTCCTCA CCCACACCAT CAGCCGCATT 
GCAGTCTCCT ACCAGACCAA GGTCAACATC CTCTCTGCCA TCAAGAGCCC TTGCCACAGG 
GAGACCCTAG AGGGGGCTGA GGCCAAGCCC TGGTACGAAC CCATCTACCA GGGAGGGGTC 
TTCCAGCTGG AGAAGGGAGA TCGCCTCAGT GCTGAGATCA ACCTGCCGGA ATACCTGGAC 
TATGCCGAGT CTGGGCAGGT CTACTTTGGG ATCATCGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_012100437.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011955827.1 Ovis aries tumor necrosis factor (TNF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm21918
Clone ID Related Accession (Same CDS sequence)	XM_012100437.2
Accession Version	XM_012100437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	tumor necrosis factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCCT TTCCCCGCCC TCTTCTCACC CGACAGCGTA TAAATGCAGC TGTTTGCACA 
CCCAGCCAAC AGAAGCTCCC AGAGCGGGGA CACCAGGGGA CCAGCCAAGA GAGAGACAAG 
CAGCTGCAGA ACCCCCTGGA GATAACCTCC CAGACAACAC ACCCCCGAGA GACAGCCAGG 
CAACTTGCTC TCTCATACAC CCTGCCACAA GGCTCTCCTG TCTCCCGTCT GGACTTGGAT 
CCTTCTGAAA AAGACACCAT GAGCACCAAA AGCATGATCC GGGATGTGGA GCTGGCGGAG 
GAGGTGCTCT CCAACAAAGC AGGGGGCCCC CAGGGCTCCA GAAGTTGCTG GTGCCTCAGC 
CTCTTCTCCT TCCTCCTGGT TGCAGGAGCC ACCACGCTCT TCTGCCTGCT GCACTTCGGG 
GTAATCGGCC CCCAGAGGGA AGAGTCCCCA GCTGGCCCCT CCTTCAACAG GCCTCTGGTT 
CAGACACTCA GGTCATCTTC TCAAGCCTCA AATAACAAGC CGGTAGCCCA CGTTGTAGCC 
AACATCAGCG CTCCGGGGCA GCTCCGATGG GGGGACTCGT ATGCCAATGC CCTCATGGCC 
AACGGCGTGG AGCTGAAAGA CAACCAGCTG GTGGTGCCCA CTGACGGGCT TTACCTCATC 
TACTCGCAGG TCCTCTTCAG GGGCCACGGC TGCCCTTCCA CCCCCTTGTT CCTCACCCAC 
ACCATCAGCC GCATTGCAGT CTCCTACCAG ACCAAGGTCA ACATCCTCTC TGCCATCAAG 
AGCCCTTGCC ACAGGGAGAC CCTAGAGGGG GCTGAGGCCA AGCCCTGGTA CGAACCCATC 
TACCAGGGAG GGGTCTTCCA GCTGGAGAAG GGAGATCGCC TCAGTGCTGA GATCAACCTG 
CCGGAATACC TGGACTATGC CGAGTCTGGG CAGGTCTACT TTGGGATCAT CGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011955827.1
CDS	1791..2750
Translation	MRAFPRPLLTRQRINAAVCTPSQQKLPERGHQGTSQERDKQLQNPLEITSQTTHPRETARQLALSYTLPQGSPVSRLDLDPSEKDTMSTKSMIRDVELAEEVLSNKAGGPQGSRSCWCLSLFSFLLVAGATTLFCLLHFGVIGPQREESPAGPSFNRPLVQTLRSSSQASNNKPVAHVVANISAPGQLRWGDSYANALMANGVELKDNQLVVPTDGLYLIYSQVLFRGHGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETLEGAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPEYLDYAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_012100437.2
Organism	Ovis aries(sheep)
Definition	Ovis aries tumor necrosis factor (TNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100437.2

ORF Insert Sequence:

ATGAGAGCCT TTCCCCGCCC TCTTCTCACC CGACAGCGTA TAAATGCAGC TGTTTGCACA 
CCCAGCCAAC AGAAGCTCCC AGAGCGGGGA CACCAGGGGA CCAGCCAAGA GAGAGACAAG 
CAGCTGCAGA ACCCCCTGGA GATAACCTCC CAGACAACAC ACCCCCGAGA GACAGCCAGG 
CAACTTGCTC TCTCATACAC CCTGCCACAA GGCTCTCCTG TCTCCCGTCT GGACTTGGAT 
CCTTCTGAAA AAGACACCAT GAGCACCAAA AGCATGATCC GGGATGTGGA GCTGGCGGAG 
GAGGTGCTCT CCAACAAAGC AGGGGGCCCC CAGGGCTCCA GAAGTTGCTG GTGCCTCAGC 
CTCTTCTCCT TCCTCCTGGT TGCAGGAGCC ACCACGCTCT TCTGCCTGCT GCACTTCGGG 
GTAATCGGCC CCCAGAGGGA AGAGTCCCCA GCTGGCCCCT CCTTCAACAG GCCTCTGGTT 
CAGACACTCA GGTCATCTTC TCAAGCCTCA AATAACAAGC CGGTAGCCCA CGTTGTAGCC 
AACATCAGCG CTCCGGGGCA GCTCCGATGG GGGGACTCGT ATGCCAATGC CCTCATGGCC 
AACGGCGTGG AGCTGAAAGA CAACCAGCTG GTGGTGCCCA CTGACGGGCT TTACCTCATC 
TACTCGCAGG TCCTCTTCAG GGGCCACGGC TGCCCTTCCA CCCCCTTGTT CCTCACCCAC 
ACCATCAGCC GCATTGCAGT CTCCTACCAG ACCAAGGTCA ACATCCTCTC TGCCATCAAG 
AGCCCTTGCC ACAGGGAGAC CCTAGAGGGG GCTGAGGCCA AGCCCTGGTA CGAACCCATC 
TACCAGGGAG GGGTCTTCCA GCTGGAGAAG GGAGATCGCC TCAGTGCTGA GATCAACCTG 
CCGGAATACC TGGACTATGC CGAGTCTGGG CAGGTCTACT TTGGGATCAT CGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_012100437.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011955827.2 Ovis aries tumor necrosis factor (TNF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm71317
Clone ID Related Accession (Same CDS sequence)	XM_012100437.3
Accession Version	XM_012100437.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	tumor necrosis factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040271.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012100437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCAACAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TGGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGTCCC CAGCTGGCCC CTCCTTCAAC AGGCCTCTGG TTCAGACACT CAGGTCATCT 
TCTCAAGCCT CAAATAACAA GCCGGTAGCC CACGTTGTAG CCAACATCAG CGCTCCGGGG 
CAGCTCCGAT GGGGGGACTC GTATGCCAAT GCCCTCATGG CCAACGGCGT GGAGCTGAAA 
GACAACCAGC TGGTGGTGCC CACTGACGGG CTTTACCTCA TCTACTCGCA GGTCCTCTTC 
AGGGGCCACG GCTGCCCTTC CACCCCCTTG TTCCTCACCC ACACCATCAG CCGCATTGCA 
GTCTCCTACC AGACCAAGGT CAACATCCTC TCTGCCATCA AGAGCCCTTG CCACAGGGAG 
ACCCTAGAGG GGGCTGAGGC CAAGCCCTGG TACGAACCCA TCTACCAGGG AGGGGTCTTC 
CAGCTGGAGA AGGGAGATCG CCTCAGTGCT GAGATCAACC TGCCGGAATA CCTGGACTAT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATCGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011955827.2
CDS	200..901
Translation	MSTKSMIRDVELAEEVLSNKAGGPQGSRSCWCLSLFSFLLVAGATTLFCLLHFGVIGPQREESPAGPSFNRPLVQTLRSSSQASNNKPVAHVVANISAPGQLRWGDSYANALMANGVELKDNQLVVPTDGLYLIYSQVLFRGHGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETLEGAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPEYLDYAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_012100437.3
Organism	Ovis aries(sheep)
Definition	Ovis aries tumor necrosis factor (TNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100437.3

ORF Insert Sequence:

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCAACAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TGGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGTCCC CAGCTGGCCC CTCCTTCAAC AGGCCTCTGG TTCAGACACT CAGGTCATCT 
TCTCAAGCCT CAAATAACAA GCCGGTAGCC CACGTTGTAG CCAACATCAG CGCTCCGGGG 
CAGCTCCGAT GGGGGGACTC GTATGCCAAT GCCCTCATGG CCAACGGCGT GGAGCTGAAA 
GACAACCAGC TGGTGGTGCC CACTGACGGG CTTTACCTCA TCTACTCGCA GGTCCTCTTC 
AGGGGCCACG GCTGCCCTTC CACCCCCTTG TTCCTCACCC ACACCATCAG CCGCATTGCA 
GTCTCCTACC AGACCAAGGT CAACATCCTC TCTGCCATCA AGAGCCCTTG CCACAGGGAG 
ACCCTAGAGG GGGCTGAGGC CAAGCCCTGG TACGAACCCA TCTACCAGGG AGGGGTCTTC 
CAGCTGGAGA AGGGAGATCG CCTCAGTGCT GAGATCAACC TGCCGGAATA CCTGGACTAT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATCGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tumor Necrosis Factor-?, Interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in Ossimi sheep with encephalitic listeriosis.
Cytokine73(2)283-7(2015 Jun)
Abdlla OA,Elboshy ME,Reisha EF,Gadlla HA,El-Khodery SA

Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?
Spine39(13)1010-7(2014 Jun)
Hodges PW,James G,Blomster L,Hall L,Schmid AB,Shu C,Little C,Melrose J

Peripheral injection of SB203580 inhibits the inflammatory-dependent synthesis of proinflammatory cytokines in the hypothalamus.
BioMed research international2014475152(2014)
Herman AP,Krawczy?ska A,Bochenek J,Antushevich H,Herman A,Tomaszewska-Zaremba D

Tumor necrosis factor-alpha and acute-phase proteins in early pregnant ewes after challenge with peptidoglycan-polysaccharide.
Domestic animal endocrinology39(2)147-54(2010 Aug)
Dow TL,Rogers-Nieman G,Hol?skov? I,Elsasser TH,Dailey RA

Elevated plasma levels of interleukin 1 beta, tumour necrosis factor alpha and monocyte chemotactic protein 1 are associated with pregnancy toxaemia in ewes.
Veterinary research communications31(5)565-73(2007 Jul)
Yarim GF,Karahan S,Nisbet C