GPX4 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPX4
Entrez Gene ID	705333
Full Name	glutathione peroxidase 4
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)
Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001118889.2	NP_001112361.1	phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A precursor
NM_001346420.1	NP_001333349.1	phospholipid hydroperoxide glutathione peroxidase isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPX4	NP_002076.2
Bos taurus (cattle)	GPX4	NP_777195.1
Caenorhabditis elegans (roundworm)	R03G5.5	NP_001123171.1
Danio rerio (zebrafish)	gpx4b	NP_001025241.2
Xenopus tropicalis (tropical clawed frog)	gpx4	XP_002937424.2
	GPX4	NP_001112361.1
Saccharomyces cerevisiae (baker's yeast)	GPX2	NP_009803.3
Rattus norvegicus (Norway rat)	Gpx4	NP_058861.3
Arabidopsis thaliana (thale cress)	GPX1	NP_180080.1
Arabidopsis thaliana (thale cress)	GPX7	NP_194915.2
Mus musculus (house mouse)	Gpx4	NP_001032830.2
Caenorhabditis elegans (roundworm)	T09A12.2	NP_001023368.1

Gene Ontology
Bibliography

Related articles in PubMed

A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Zimin AV, Cornish AS, Maudhoo MD, Gibbs RM, Zhang X, Pandey S, Meehan DT, Wipfler K, Bosinger SE, Johnson ZP, Tharp GK, Mar?ais G, Roberts M, Ferguson B, Fox HS, Treangen T, Salzberg SL, Yorke JA, Norgren RB Jr
Biology direct9(1)20(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Analysis of the Macaca mulatta transcriptome and the sequence divergence between Macaca and human.
Magness CL, Fellin PC, Thomas MJ, Korth MJ, Agy MB, Proll SC, Fitzgibbon M, Scherer CA, Miner DG, Katze MG, Iadonato SP
Genome biology6(7)R60(2005)

Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GPX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMb02727	NM_001118889.2 Latest version!	Macaca mulatta glutathione peroxidase 4 (GPX4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OMb57578	NM_001346420.1 Latest version!	Macaca mulatta glutathione peroxidase 4 (GPX4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPX4 ( NM_001118889.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001112361.1 Macaca mulatta glutathione peroxidase 4 (GPX4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMb02727
Clone ID Related Accession (Same CDS sequence)	NM_001118889.2
Accession Version	NM_001118889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Macaca mulatta(Rhesus monkey)
Product	phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from JV649198.1, JV681449.1 and JV465885.1. On Oct 11, 2016 this sequence version replaced NM_001118889.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes the canonical isoform A. A similar isoform in rat has been shown to be localized to the mitochondria (PMID:9988735). ##Evidence-Data-START## Transcript exon combination :: JV649198.1, JV681449.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4688315, SAMEA4688316 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACCTCG GCCGCCTTTG CCGCCTGCTG AAGCCGGCGC TGCTCTGTGG GGCTCTGGCC 
GCGCCCGGCC TCGCCGGTAC CATGTGCGCG TCCCGGGACG ACTGGCGCTG TGCGCGCTCC 
ATGCACGAGT TTTCCGCGAA AGACATCGAC GGGCACATGG TTAACCTGGA CAAGTACCGG 
GGGTTCGTGT GCATCGTCAC CAACGTGGCC TCCCAGTGAG GCAAGACCGA AGTGAACTAC 
ACTCAGCTCG TCGACCTGCA CGCCCGATAC GCTGAGTGCG GTTTGCGGAT CCTGGCCTTC 
CCATGTAACC AGTTCGGGAA GCAGGAGCCA GGGAGTAACG AAGAGATCAA AGAGTTCGCC 
GCAGGCTACA ACGTCAAATT CGATATGTTC AGCAAGATAT GCGTGAACGG GGACGATGCC 
CACCCGCTGT GGAAGTGGAT GAAGATCCAG CCCAAGGGCA AGGGCATCCT GGGAAATGCC 
ATCAAGTGGA ACTTCACCAA GTTCCTCATT GACAAGAACG GCTGCGTGGT GAAGCGGTAC 
GGACCCATGG AGGAACCCCT GGTGATAGAG AAGGACCTGC CCCACTATTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001112361.1
CDS	73..666
Translation	MNLGRLCRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQ*GKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF

Target ORF information:

RefSeq Version	NM_001118889.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta glutathione peroxidase 4 (GPX4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001118889.2

ORF Insert Sequence:

ATGAACCTCG GCCGCCTTTG CCGCCTGCTG AAGCCGGCGC TGCTCTGTGG GGCTCTGGCC 
GCGCCCGGCC TCGCCGGTAC CATGTGCGCG TCCCGGGACG ACTGGCGCTG TGCGCGCTCC 
ATGCACGAGT TTTCCGCGAA AGACATCGAC GGGCACATGG TTAACCTGGA CAAGTACCGG 
GGGTTCGTGT GCATCGTCAC CAACGTGGCC TCCCAGTGAG GCAAGACCGA AGTGAACTAC 
ACTCAGCTCG TCGACCTGCA CGCCCGATAC GCTGAGTGCG GTTTGCGGAT CCTGGCCTTC 
CCATGTAACC AGTTCGGGAA GCAGGAGCCA GGGAGTAACG AAGAGATCAA AGAGTTCGCC 
GCAGGCTACA ACGTCAAATT CGATATGTTC AGCAAGATAT GCGTGAACGG GGACGATGCC 
CACCCGCTGT GGAAGTGGAT GAAGATCCAG CCCAAGGGCA AGGGCATCCT GGGAAATGCC 
ATCAAGTGGA ACTTCACCAA GTTCCTCATT GACAAGAACG GCTGCGTGGT GAAGCGGTAC 
GGACCCATGG AGGAACCCCT GGTGATAGAG AAGGACCTGC CCCACTATTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPX4 ( NM_001346420.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001333349.1 Macaca mulatta glutathione peroxidase 4 (GPX4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMb57578
Clone ID Related Accession (Same CDS sequence)	NM_001346420.1
Accession Version	NM_001346420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Macaca mulatta(Rhesus monkey)
Product	phospholipid hydroperoxide glutathione peroxidase isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from JV602446.1 and JV465885.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. The resulting isoform (B) has a longer and a distinct N-terminus compared to isoform A. ##Evidence-Data-START## Transcript exon combination :: JV602446.1, JV667383.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4688316, SAMEA4688317 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGCG CGGGCGCAGG CTCCCCCAGG CGCCGCAGGC AGCGGTGCCA GAGCCGGGGC 
AGACGGCGGC CGCGAGCCCC TCGACGGCGG AAGGCCCCAG CGTGCAGGCG CAGGAGGGCG 
CGGCGCCGGC GGAAGGAGCC CTGTCCCCGC AGCTTGGGAC CGGAGATCCA CGAATGTCCC 
GAATCCCAGG ACCCGTGCGC GTCCCGGGAC GACTGGCGCT GTGCGCGCTC CATGCACGAG 
TTTTCCGCGA AAGACATCGA CGGGCACATG GTTAACCTGG ACAAGTACCG GGGGTTCGTG 
TGCATCGTCA CCAACGTGGC CTCCCAGTGA GGCAAGACCG AAGTGAACTA CACTCAGCTC 
GTCGACCTGC ACGCCCGATA CGCTGAGTGC GGTTTGCGGA TCCTGGCCTT CCCATGTAAC 
CAGTTCGGGA AGCAGGAGCC AGGGAGTAAC GAAGAGATCA AAGAGTTCGC CGCAGGCTAC 
AACGTCAAAT TCGATATGTT CAGCAAGATA TGCGTGAACG GGGACGATGC CCACCCGCTG 
TGGAAGTGGA TGAAGATCCA GCCCAAGGGC AAGGGCATCC TGGGAAATGC CATCAAGTGG 
AACTTCACCA AGTTCCTCAT TGACAAGAAC GGCTGCGTGG TGAAGCGGTA CGGACCCATG 
GAGGAACCCC TGGTGATAGA GAAGGACCTG CCCCACTATT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333349.1
CDS	90..794
Translation	MGRAGAGSPRRRRQRCQSRGRRRPRAPRRRKAPACRRRRARRRRKEPCPRSLGPEIHECPESQDPCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQ*GKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF

Target ORF information:

RefSeq Version	NM_001346420.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta glutathione peroxidase 4 (GPX4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346420.1

ORF Insert Sequence:

ATGGGCCGCG CGGGCGCAGG CTCCCCCAGG CGCCGCAGGC AGCGGTGCCA GAGCCGGGGC 
AGACGGCGGC CGCGAGCCCC TCGACGGCGG AAGGCCCCAG CGTGCAGGCG CAGGAGGGCG 
CGGCGCCGGC GGAAGGAGCC CTGTCCCCGC AGCTTGGGAC CGGAGATCCA CGAATGTCCC 
GAATCCCAGG ACCCGTGCGC GTCCCGGGAC GACTGGCGCT GTGCGCGCTC CATGCACGAG 
TTTTCCGCGA AAGACATCGA CGGGCACATG GTTAACCTGG ACAAGTACCG GGGGTTCGTG 
TGCATCGTCA CCAACGTGGC CTCCCAGTGA GGCAAGACCG AAGTGAACTA CACTCAGCTC 
GTCGACCTGC ACGCCCGATA CGCTGAGTGC GGTTTGCGGA TCCTGGCCTT CCCATGTAAC 
CAGTTCGGGA AGCAGGAGCC AGGGAGTAAC GAAGAGATCA AAGAGTTCGC CGCAGGCTAC 
AACGTCAAAT TCGATATGTT CAGCAAGATA TGCGTGAACG GGGACGATGC CCACCCGCTG 
TGGAAGTGGA TGAAGATCCA GCCCAAGGGC AAGGGCATCC TGGGAAATGC CATCAAGTGG 
AACTTCACCA AGTTCCTCAT TGACAAGAAC GGCTGCGTGG TGAAGCGGTA CGGACCCATG 
GAGGAACCCC TGGTGATAGA GAAGGACCTG CCCCACTATT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Biology direct9(1)20(2014 Oct)
Zimin AV,Cornish AS,Maudhoo MD,Gibbs RM,Zhang X,Pandey S,Meehan DT,Wipfler K,Bosinger SE,Johnson ZP,Tharp GK,Mar?ais G,Roberts M,Ferguson B,Fox HS,Treangen T,Salzberg SL,Yorke JA,Norgren RB Jr

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Analysis of the Macaca mulatta transcriptome and the sequence divergence between Macaca and human.
Genome biology6(7)R60(2005)
Magness CL,Fellin PC,Thomas MJ,Korth MJ,Agy MB,Proll SC,Fitzgibbon M,Scherer CA,Miner DG,Katze MG,Iadonato SP

Characterization of mammalian selenoproteomes.
Science (New York, N.Y.)300(5624)1439-43(2003 May)
Kryukov GV,Castellano S,Novoselov SV,Lobanov AV,Zehtab O,Guig? R,Gladyshev VN

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GPX4 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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