Gpx4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpx4
Entrez Gene ID	29328
Full Name	glutathione peroxidase 4
Synonyms	Gshpx-4,Phgpx,gpx-4,snGpx
General protein information	Preferred Names glutathione peroxidase 4 Names phospholipid hydroperoxide glutathione peroxidase, nuclear phospholipid hydroperoxide glutathione peroxidase, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants encoding isoforms with different subcellular localization have been described for this gene. Pseudogenes of this locus have been identified on chromosomes 1, 10 and 14. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039849.2	NP_001034938.1	phospholipid hydroperoxide glutathione peroxidase isoform B
NM_017165.3	NP_058861.3	phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A precursor
NM_001039849.3	NP_001034938.1	phospholipid hydroperoxide glutathione peroxidase isoform B
NM_001368043.1	NP_001354972.1	phospholipid hydroperoxide glutathione peroxidase isoform C
NM_017165.4	NP_058861.3	phospholipid hydroperoxide glutathione peroxidase isoform A precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPX4	NP_002076.2
Bos taurus (cattle)	GPX4	NP_777195.1
Caenorhabditis elegans (roundworm)	R03G5.5	NP_001123171.1
Danio rerio (zebrafish)	gpx4b	NP_001025241.2
Xenopus tropicalis (tropical clawed frog)	gpx4	XP_002937424.2
	GPX4	NP_001112361.1
Saccharomyces cerevisiae (baker's yeast)	GPX2	NP_009803.3
Rattus norvegicus (Norway rat)	Gpx4	NP_058861.3
Arabidopsis thaliana (thale cress)	GPX1	NP_180080.1
Arabidopsis thaliana (thale cress)	GPX7	NP_194915.2
Mus musculus (house mouse)	Gpx4	NP_001032830.2
Caenorhabditis elegans (roundworm)	T09A12.2	NP_001023368.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis.
Ingold I, Berndt C, Schmitt S, Doll S, Poschmann G, Buday K, Roveri A, Peng X, Porto Freitas F, Seibt T, Mehr L, Aichler M, Walch A, Lamp D, Jastroch M, Miyamoto S, Wurst W, Ursini F, Arn?r ESJ, Fradejas-Villar N, Schweizer U, Zischka H, Friedmann Angeli JP, Conrad M
Cell(2017 Dec)

Glutathione peroxidase 4-catalyzed reduction of lipid hydroperoxides in membranes: The polar head of membrane phospholipids binds the enzyme and addresses the fatty acid hydroperoxide group toward the redox center.
Cozza G, Rossetto M, Bosello-Travain V, Maiorino M, Roveri A, Toppo S, Zaccarin M, Zennaro L, Ursini F
Free radical biology & medicine1121-11(2017 Nov)

Selenocysteine oxidation in glutathione peroxidase catalysis: an MS-supported quantum mechanics study.
Orian L, Mauri P, Roveri A, Toppo S, Benazzi L, Bosello-Travain V, De Palma A, Maiorino M, Miotto G, Zaccarin M, Polimeno A, Floh? L, Ursini F
Free radical biology & medicine871-14(2015 Oct)

Influence of SkQ1 on Expression of Nrf2 Transcription Factor Gene, ARE-Controlled Genes of Antioxidant Enzymes and Their Activity in Rat Blood Leukocytes.
Vnukov VV, Gutsenko OI, Milutina NP, Ananyan AA, Danilenko AO, Panina SB, Kornienko IV
Biochemistry. Biokhimiia80(5)586-91(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MS/MS analysis of oxidized rat GPx4 complies with the predicted structure, an 8-membered ring, in which selenium is bound as selenenylamide to the protein backbone.
Title: Selenocysteine oxidation in glutathione peroxidase catalysis: an MS-supported quantum mechanics study.

mitochondria-targeted antioxidant SkQ1 (50 nmol/kg during 5 days) significantly increased the mRNA levels of Nrf2 transcription factor and Nrf2-induced genes encoding antioxidant enzymes SOD1, SOD2, CAT, and GPx4 in rat peripheral blood leukocytes.
Title: Influence of SkQ1 on Expression of Nrf2 Transcription Factor Gene, ARE-Controlled Genes of Antioxidant Enzymes and Their Activity in Rat Blood Leukocytes.

Our findings suggest that a decrease in mitochondrial GPx4 monomer and a functional loss of activity may be a contributing factor to the vulnerability of dopaminergic neurons in Parkinson's disease
Title: Dopamine quinone modifies and decreases the abundance of the mitochondrial selenoprotein glutathione peroxidase 4.

Upon selenium fluctuation, the modulation of UGA recoding efficiency depends on the nature of the SECIS, with Gpx1 being more sensitive than Gpx4.
Title: The differential expression of glutathione peroxidase 1 and 4 depends on the nature of the SECIS element.

Data show that the intake of fish oil in the selenium-deficient animal led to a markedly altered formation of sperm nuclei glutathione peroxidase (snGPx), also designated as the nuclear form of PHGPx (n-PHGPx).
Title: Studies with regard to the apoptosis of testicular germ cells in rats fed a diet enriched with polyunsaturated Fatty acids.

The following Gpx4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpx4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12528	NM_017165.4 Latest version!	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa63315	NM_001368043.1 Latest version!	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ORa12314	NM_001039849.3 Latest version!	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
ORa12314	NM_001039849.2	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
ORa12528	NM_017165.3	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpx4 ( NM_017165.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058861.3 Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12528
Clone ID Related Accession (Same CDS sequence)	NM_017165.3 , NM_017165.4
Accession Version	NM_017165.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB582508.1 and L24896.1. On Oct 7, 2016 this sequence version replaced NM_017165.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes the canonical isoform A (also known as phGPx), which is localized to the mitochondria (PMID:9988735). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U37427.1, FQ231108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN01906347 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 9988735 multifunctional gene product(s) :: PMID: 10464096 protein contains selenocysteine :: PMID: 8749327 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG GCCGTCTGAG CCGCTTATTG AAGCCAGCAC TGCTGTGCGG GGCTCTGGCT 
GTGCCTGGCC TGGCTGGCAC CATGTGTGCA TCCCGCGATG ATTGGCGCTG TGCGCGCTCC 
ATGCACGAAT TCGCAGCCAA GGACATCGAT GGGCACATGG TTTGCCTGGA TAAGTACAGG 
GGTTGCGTGT GCATCGTCAC CAACGTGGCC TCGCAATGAG GCAAAACCGA CGTAAACTAC 
ACTCAGCTAG TCGATCTGCA TGCCCGATAC GCCGAGTGTG GTTTACGAAT CCTGGCCTTC 
CCTTGCAACC AGTTCGGGAG GCAGGAGCCA GGAAGTAATC AAGAAATCAA GGAGTTTGCA 
GCCGGCTACA ATGTCAGGTT TGACATGTAC AGCAAGATCT GTGTAAATGG GGACGATGCC 
CACCCACTGT GGAAATGGAT GAAAGTCCAG CCCAAGGGCA GGGGCATGCT GGGAAATGCC 
ATCAAATGGA ACTTTACCAA GTTTCTCATT GATAAGAACG GCTGCGTGGT GAAGCGCTAT 
GGTCCCATGG AGGAGCCCCA GGTGATAGAG AAGGACCTGC CGTGCTATCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058861.3
CDS	91..684
Translation	MSWGRLSRLLKPALLCGALAVPGLAGTMCASRDDWRCARSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVASQ*GKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVRFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL

Target ORF information:

RefSeq Version	NM_017165.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017165.3

ORF Insert Sequence:

ATGAGCTGGG GCCGTCTGAG CCGCTTATTG AAGCCAGCAC TGCTGTGCGG GGCTCTGGCT 
GTGCCTGGCC TGGCTGGCAC CATGTGTGCA TCCCGCGATG ATTGGCGCTG TGCGCGCTCC 
ATGCACGAAT TCGCAGCCAA GGACATCGAT GGGCACATGG TTTGCCTGGA TAAGTACAGG 
GGTTGCGTGT GCATCGTCAC CAACGTGGCC TCGCAATGAG GCAAAACCGA CGTAAACTAC 
ACTCAGCTAG TCGATCTGCA TGCCCGATAC GCCGAGTGTG GTTTACGAAT CCTGGCCTTC 
CCTTGCAACC AGTTCGGGAG GCAGGAGCCA GGAAGTAATC AAGAAATCAA GGAGTTTGCA 
GCCGGCTACA ATGTCAGGTT TGACATGTAC AGCAAGATCT GTGTAAATGG GGACGATGCC 
CACCCACTGT GGAAATGGAT GAAAGTCCAG CCCAAGGGCA GGGGCATGCT GGGAAATGCC 
ATCAAATGGA ACTTTACCAA GTTTCTCATT GATAAGAACG GCTGCGTGGT GAAGCGCTAT 
GGTCCCATGG AGGAGCCCCA GGTGATAGAG AAGGACCTGC CGTGCTATCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpx4 ( NM_017165.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058861.3 Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12528
Clone ID Related Accession (Same CDS sequence)	NM_017165.3 , NM_017165.4
Accession Version	NM_017165.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid hydroperoxide glutathione peroxidase isoform A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07073280.1. On Jan 7, 2019 this sequence version replaced NM_017165.3. Transcript Variant: This variant (1) represents the predominant transcript and encodes the canonical isoform A (also known as phGPx or the L-form), which is localized to the mitochondria (PMID:9988735). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U37427.1, FQ231108.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 9988735 multifunctional gene product(s) :: PMID: 10464096 protein contains selenocysteine :: PMID: 8749327 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG GCCGTCTGAG CCGCTTATTG AAGCCAGCAC TGCTGTGCGG GGCTCTGGCT 
GTGCCTGGCC TGGCTGGCAC CATGTGTGCA TCCCGCGATG ATTGGCGCTG TGCGCGCTCC 
ATGCACGAAT TCGCAGCCAA GGACATCGAT GGGCACATGG TTTGCCTGGA TAAGTACAGG 
GGTTGCGTGT GCATCGTCAC CAACGTGGCC TCGCAATGAG GCAAAACCGA CGTAAACTAC 
ACTCAGCTAG TCGATCTGCA TGCCCGATAC GCCGAGTGTG GTTTACGAAT CCTGGCCTTC 
CCTTGCAACC AGTTCGGGAG GCAGGAGCCA GGAAGTAATC AAGAAATCAA GGAGTTTGCA 
GCCGGCTACA ATGTCAGGTT TGACATGTAC AGCAAGATCT GTGTAAATGG GGACGATGCC 
CACCCACTGT GGAAATGGAT GAAAGTCCAG CCCAAGGGCA GGGGCATGCT GGGAAATGCC 
ATCAAATGGA ACTTTACCAA GTTTCTCATT GATAAGAACG GCTGCGTGGT GAAGCGCTAT 
GGTCCCATGG AGGAGCCCCA GGTGATAGAG AAGGACCTGC CGTGCTATCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058861.3
CDS	67..660
Translation	MSWGRLSRLLKPALLCGALAVPGLAGTMCASRDDWRCARSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVASQ*GKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVRFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL

Target ORF information:

RefSeq Version	NM_017165.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017165.4

ORF Insert Sequence:

ATGAGCTGGG GCCGTCTGAG CCGCTTATTG AAGCCAGCAC TGCTGTGCGG GGCTCTGGCT 
GTGCCTGGCC TGGCTGGCAC CATGTGTGCA TCCCGCGATG ATTGGCGCTG TGCGCGCTCC 
ATGCACGAAT TCGCAGCCAA GGACATCGAT GGGCACATGG TTTGCCTGGA TAAGTACAGG 
GGTTGCGTGT GCATCGTCAC CAACGTGGCC TCGCAATGAG GCAAAACCGA CGTAAACTAC 
ACTCAGCTAG TCGATCTGCA TGCCCGATAC GCCGAGTGTG GTTTACGAAT CCTGGCCTTC 
CCTTGCAACC AGTTCGGGAG GCAGGAGCCA GGAAGTAATC AAGAAATCAA GGAGTTTGCA 
GCCGGCTACA ATGTCAGGTT TGACATGTAC AGCAAGATCT GTGTAAATGG GGACGATGCC 
CACCCACTGT GGAAATGGAT GAAAGTCCAG CCCAAGGGCA GGGGCATGCT GGGAAATGCC 
ATCAAATGGA ACTTTACCAA GTTTCTCATT GATAAGAACG GCTGCGTGGT GAAGCGCTAT 
GGTCCCATGG AGGAGCCCCA GGTGATAGAG AAGGACCTGC CGTGCTATCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpx4 ( NM_001368043.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001354972.1 Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa63315
Clone ID Related Accession (Same CDS sequence)	NM_001368043.1
Accession Version	NM_001368043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid hydroperoxide glutathione peroxidase isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07073280.1. Transcript Variant: This variant (3) uses an alternate promoter, which results in translation initiation from an in-frame downstream AUG start codon compared to variant 1. The encoded isoform (C, also known as the S-form) has a shorter N-terminus compared to isoform A and is localized in the cytoplasm (PMIDs:7592947, 9988735). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ219811.1, FQ221760.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 9988735 multifunctional gene product(s) :: PMID: 10464096 protein contains selenocysteine :: PMID: 8749327 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGTGCAT CCCGCGATGA TTGGCGCTGT GCGCGCTCCA TGCACGAATT CGCAGCCAAG 
GACATCGATG GGCACATGGT TTGCCTGGAT AAGTACAGGG GTTGCGTGTG CATCGTCACC 
AACGTGGCCT CGCAATGAGG CAAAACCGAC GTAAACTACA CTCAGCTAGT CGATCTGCAT 
GCCCGATACG CCGAGTGTGG TTTACGAATC CTGGCCTTCC CTTGCAACCA GTTCGGGAGG 
CAGGAGCCAG GAAGTAATCA AGAAATCAAG GAGTTTGCAG CCGGCTACAA TGTCAGGTTT 
GACATGTACA GCAAGATCTG TGTAAATGGG GACGATGCCC ACCCACTGTG GAAATGGATG 
AAAGTCCAGC CCAAGGGCAG GGGCATGCTG GGAAATGCCA TCAAATGGAA CTTTACCAAG 
TTTCTCATTG ATAAGAACGG CTGCGTGGTG AAGCGCTATG GTCCCATGGA GGAGCCCCAG 
GTGATAGAGA AGGACCTGCC GTGCTATCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354972.1
CDS	56..568
Translation	MCASRDDWRCARSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVASQ*GKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVRFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL

Target ORF information:

RefSeq Version	NM_001368043.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368043.1

ORF Insert Sequence:

ATGTGTGCAT CCCGCGATGA TTGGCGCTGT GCGCGCTCCA TGCACGAATT CGCAGCCAAG 
GACATCGATG GGCACATGGT TTGCCTGGAT AAGTACAGGG GTTGCGTGTG CATCGTCACC 
AACGTGGCCT CGCAATGAGG CAAAACCGAC GTAAACTACA CTCAGCTAGT CGATCTGCAT 
GCCCGATACG CCGAGTGTGG TTTACGAATC CTGGCCTTCC CTTGCAACCA GTTCGGGAGG 
CAGGAGCCAG GAAGTAATCA AGAAATCAAG GAGTTTGCAG CCGGCTACAA TGTCAGGTTT 
GACATGTACA GCAAGATCTG TGTAAATGGG GACGATGCCC ACCCACTGTG GAAATGGATG 
AAAGTCCAGC CCAAGGGCAG GGGCATGCTG GGAAATGCCA TCAAATGGAA CTTTACCAAG 
TTTCTCATTG ATAAGAACGG CTGCGTGGTG AAGCGCTATG GTCCCATGGA GGAGCCCCAG 
GTGATAGAGA AGGACCTGCC GTGCTATCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpx4 ( NM_001039849.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001034938.1 Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12314
Clone ID Related Accession (Same CDS sequence)	NM_001039849.2 , NM_001039849.3
Accession Version	NM_001039849.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid hydroperoxide glutathione peroxidase isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FQ087247.1, AB072798.1 and L24896.1. On Jan 22, 2014 this sequence version replaced NM_001039849.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. The resulting isoform (B, also know as snGPx) has a longer and a distinct N-terminus compared to isoform A. This isoform has been reported to be localized in the sperm nucleus (PMID:11344099). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB072798.1, AY570513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN00632258 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 9988735 multifunctional gene product(s) :: PMID: 10464096 protein contains selenocysteine :: PMID: 8749327 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGCG CGGCCGCCCG CAAGCGGGGA CGCTGCAGAC AGCGCGGCCG GTCCCCGGGA 
GGCCGGCGAC GGCGTGAACC TGGACGCCAA AGTCCTAGGA AGCGCCCAGG CCCTCGGAGG 
AGGAGAGCTC GCGCGCGCCG CCGCAGGAGG GCGCGCCCTC GCCGGATGGA GCCCATTCCC 
GAGCCTTTCA ACCCGCGGCC TCTGCTGCAG GACCTTCCCC AGACCAGCAA CAGCCACGAG 
TTCCTGGGCT TGTGTGCATC CCGCGATGAT TGGCGCTGTG CGCGCTCCAT GCACGAATTC 
GCAGCCAAGG ACATCGATGG GCACATGGTT TGCCTGGATA AGTACAGGGG TTGCGTGTGC 
ATCGTCACCA ACGTGGCCTC GCAATGAGGC AAAACCGACG TAAACTACAC TCAGCTAGTC 
GATCTGCATG CCCGATACGC CGAGTGTGGT TTACGAATCC TGGCCTTCCC TTGCAACCAG 
TTCGGGAGGC AGGAGCCAGG AAGTAATCAA GAAATCAAGG AGTTTGCAGC CGGCTACAAT 
GTCAGGTTTG ACATGTACAG CAAGATCTGT GTAAATGGGG ACGATGCCCA CCCACTGTGG 
AAATGGATGA AAGTCCAGCC CAAGGGCAGG GGCATGCTGG GAAATGCCAT CAAATGGAAC 
TTTACCAAGT TTCTCATTGA TAAGAACGGC TGCGTGGTGA AGCGCTATGG TCCCATGGAG 
GAGCCCCAGG TGATAGAGAA GGACCTGCCG TGCTATCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034938.1
CDS	13..774
Translation	MGRAAARKRGRCRQRGRSPGGRRRREPGRQSPRKRPGPRRRRARARRRRRARPRRMEPIPEPFNPRPLLQDLPQTSNSHEFLGLCASRDDWRCARSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVASQ*GKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVRFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL

Target ORF information:

RefSeq Version	NM_001039849.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039849.2

ORF Insert Sequence:

ATGGGCCGCG CGGCCGCCCG CAAGCGGGGA CGCTGCAGAC AGCGCGGCCG GTCCCCGGGA 
GGCCGGCGAC GGCGTGAACC TGGACGCCAA AGTCCTAGGA AGCGCCCAGG CCCTCGGAGG 
AGGAGAGCTC GCGCGCGCCG CCGCAGGAGG GCGCGCCCTC GCCGGATGGA GCCCATTCCC 
GAGCCTTTCA ACCCGCGGCC TCTGCTGCAG GACCTTCCCC AGACCAGCAA CAGCCACGAG 
TTCCTGGGCT TGTGTGCATC CCGCGATGAT TGGCGCTGTG CGCGCTCCAT GCACGAATTC 
GCAGCCAAGG ACATCGATGG GCACATGGTT TGCCTGGATA AGTACAGGGG TTGCGTGTGC 
ATCGTCACCA ACGTGGCCTC GCAATGAGGC AAAACCGACG TAAACTACAC TCAGCTAGTC 
GATCTGCATG CCCGATACGC CGAGTGTGGT TTACGAATCC TGGCCTTCCC TTGCAACCAG 
TTCGGGAGGC AGGAGCCAGG AAGTAATCAA GAAATCAAGG AGTTTGCAGC CGGCTACAAT 
GTCAGGTTTG ACATGTACAG CAAGATCTGT GTAAATGGGG ACGATGCCCA CCCACTGTGG 
AAATGGATGA AAGTCCAGCC CAAGGGCAGG GGCATGCTGG GAAATGCCAT CAAATGGAAC 
TTTACCAAGT TTCTCATTGA TAAGAACGGC TGCGTGGTGA AGCGCTATGG TCCCATGGAG 
GAGCCCCAGG TGATAGAGAA GGACCTGCCG TGCTATCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpx4 ( NM_001039849.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001034938.1 Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 2, mRNA.

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CloneID	ORa12314
Clone ID Related Accession (Same CDS sequence)	NM_001039849.2 , NM_001039849.3
Accession Version	NM_001039849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid hydroperoxide glutathione peroxidase isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07073280.1. On Jan 7, 2019 this sequence version replaced NM_001039849.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. The resulting isoform (B, also know as snGPx) has a longer and a distinct N-terminus compared to isoform A. This isoform has been reported to be localized in the sperm nucleus (PMID:11344099). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB072798.1, AY570513.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 9988735 multifunctional gene product(s) :: PMID: 10464096 protein contains selenocysteine :: PMID: 8749327 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGCG CGGCCGCCCG CAAGCGGGGA CGCTGCAGAC AGCGCGGCCG GTCCCCGGGA 
GGCCGGCGAC GGCGTGAACC TGGACGCCAA AGTCCTAGGA AGCGCCCAGG CCCTCGGAGG 
AGGAGAGCTC GCGCGCGCCG CCGCAGGAGG GCGCGCCCTC GCCGGATGGA GCCCATTCCC 
GAGCCTTTCA ACCCGCGGCC TCTGCTGCAG GACCTTCCCC AGACCAGCAA CAGCCACGAG 
TTCCTGGGCT TGTGTGCATC CCGCGATGAT TGGCGCTGTG CGCGCTCCAT GCACGAATTC 
GCAGCCAAGG ACATCGATGG GCACATGGTT TGCCTGGATA AGTACAGGGG TTGCGTGTGC 
ATCGTCACCA ACGTGGCCTC GCAATGAGGC AAAACCGACG TAAACTACAC TCAGCTAGTC 
GATCTGCATG CCCGATACGC CGAGTGTGGT TTACGAATCC TGGCCTTCCC TTGCAACCAG 
TTCGGGAGGC AGGAGCCAGG AAGTAATCAA GAAATCAAGG AGTTTGCAGC CGGCTACAAT 
GTCAGGTTTG ACATGTACAG CAAGATCTGT GTAAATGGGG ACGATGCCCA CCCACTGTGG 
AAATGGATGA AAGTCCAGCC CAAGGGCAGG GGCATGCTGG GAAATGCCAT CAAATGGAAC 
TTTACCAAGT TTCTCATTGA TAAGAACGGC TGCGTGGTGA AGCGCTATGG TCCCATGGAG 
GAGCCCCAGG TGATAGAGAA GGACCTGCCG TGCTATCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034938.1
CDS	13..774
Translation	MGRAAARKRGRCRQRGRSPGGRRRREPGRQSPRKRPGPRRRRARARRRRRARPRRMEPIPEPFNPRPLLQDLPQTSNSHEFLGLCASRDDWRCARSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVASQ*GKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVRFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL

Target ORF information:

RefSeq Version	NM_001039849.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039849.3

ORF Insert Sequence:

ATGGGCCGCG CGGCCGCCCG CAAGCGGGGA CGCTGCAGAC AGCGCGGCCG GTCCCCGGGA 
GGCCGGCGAC GGCGTGAACC TGGACGCCAA AGTCCTAGGA AGCGCCCAGG CCCTCGGAGG 
AGGAGAGCTC GCGCGCGCCG CCGCAGGAGG GCGCGCCCTC GCCGGATGGA GCCCATTCCC 
GAGCCTTTCA ACCCGCGGCC TCTGCTGCAG GACCTTCCCC AGACCAGCAA CAGCCACGAG 
TTCCTGGGCT TGTGTGCATC CCGCGATGAT TGGCGCTGTG CGCGCTCCAT GCACGAATTC 
GCAGCCAAGG ACATCGATGG GCACATGGTT TGCCTGGATA AGTACAGGGG TTGCGTGTGC 
ATCGTCACCA ACGTGGCCTC GCAATGAGGC AAAACCGACG TAAACTACAC TCAGCTAGTC 
GATCTGCATG CCCGATACGC CGAGTGTGGT TTACGAATCC TGGCCTTCCC TTGCAACCAG 
TTCGGGAGGC AGGAGCCAGG AAGTAATCAA GAAATCAAGG AGTTTGCAGC CGGCTACAAT 
GTCAGGTTTG ACATGTACAG CAAGATCTGT GTAAATGGGG ACGATGCCCA CCCACTGTGG 
AAATGGATGA AAGTCCAGCC CAAGGGCAGG GGCATGCTGG GAAATGCCAT CAAATGGAAC 
TTTACCAAGT TTCTCATTGA TAAGAACGGC TGCGTGGTGA AGCGCTATGG TCCCATGGAG 
GAGCCCCAGG TGATAGAGAA GGACCTGCCG TGCTATCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis.
Cell(2017 Dec)
Ingold I,Berndt C,Schmitt S,Doll S,Poschmann G,Buday K,Roveri A,Peng X,Porto Freitas F,Seibt T,Mehr L,Aichler M,Walch A,Lamp D,Jastroch M,Miyamoto S,Wurst W,Ursini F,Arn?r ESJ,Fradejas-Villar N,Schweizer U,Zischka H,Friedmann Angeli JP,Conrad M

Glutathione peroxidase 4-catalyzed reduction of lipid hydroperoxides in membranes: The polar head of membrane phospholipids binds the enzyme and addresses the fatty acid hydroperoxide group toward the redox center.
Free radical biology & medicine1121-11(2017 Nov)
Cozza G,Rossetto M,Bosello-Travain V,Maiorino M,Roveri A,Toppo S,Zaccarin M,Zennaro L,Ursini F

Selenocysteine oxidation in glutathione peroxidase catalysis: an MS-supported quantum mechanics study.
Free radical biology & medicine871-14(2015 Oct)
Orian L,Mauri P,Roveri A,Toppo S,Benazzi L,Bosello-Travain V,De Palma A,Maiorino M,Miotto G,Zaccarin M,Polimeno A,Floh? L,Ursini F

Influence of SkQ1 on Expression of Nrf2 Transcription Factor Gene, ARE-Controlled Genes of Antioxidant Enzymes and Their Activity in Rat Blood Leukocytes.
Biochemistry. Biokhimiia80(5)586-91(2015 May)
Vnukov VV,Gutsenko OI,Milutina NP,Ananyan AA,Danilenko AO,Panina SB,Kornienko IV

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Gpx4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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