LOC4332718 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

The following LOC4332718 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC4332718 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OOa30426 XM_015775852.2
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Oryza sativa Japonica Group guanine nucleotide exchange factor SPIKE 1 (LOC4332718), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOa30426 XM_015775853.1
Latest version!
Oryza sativa Japonica Group guanine nucleotide exchange factor SPIKE 1 (LOC4332718), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
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OOa30426 XM_015775852.1 Oryza sativa Japonica Group guanine nucleotide exchange factor SPIKE 1 (LOC4332718), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OOa30426
    Clone ID Related Accession (Same CDS sequence) XM_015775852.1 , XM_015775852.2 , XM_015775853.1
    Accession Version XM_015775852.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-02-29
    Organism Oryza sativa Japonica Group(Japanese rice)
    Product guanine nucleotide exchange factor SPIKE 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATTCCG CGGCGGCGGC GGGGGAGGGG CAGCGGTTCA AGCGCATTCC GCGGCAGTCG 
    TTGGCGGGGA ACCTGGAGCT GGATCCACTG CTTAATGAAA ATTTAGATCA ATGGCCACAT
    CTAAATGAGC TGGTTCAGTG CTATAAGGCT GATTTTGTGA AAGACGACTG CAAATATGGA
    CGATATGAAA GTGTTGCACC ACCATCTTTT CAGAATCAAA TTTTTGAGGG ACCTGATACT
    GACTTAGAAA CAGAATTGCA GCTTAGCAAT GATAGGCAAT CCAAGCCTGA TGAAGTTACT
    GAAGATGATA TGCCAAGCAC ATCAGGGAGA CAATTATATG AGACTGAAGT GCCCGCTTCA
    TCCTCAAAAA AACACTGCAG TCTATCACCT TTACCAGCAT ATGAACCGGC ATTTGATTGG
    GAAAATGAGA GATCTTTGAT ATTCGGCCAA CGGGTGCCAG AAAGTGTCCC TGCAATAAAC
    AGCAGTGGAT TGAAGATAAC TGTCAAGGTG TTATCTTTGT CATTTCAAGC TGGATTAGTT
    GAGCCTTTTA GTGGTACAAT CTGCTTGTAC AACAGAGACA GAAGAGAAAA ATTGTCAGAA
    GATTTCTATT TTCATATACT CCCAACAGAA ATGCAAGATG CTCAGATTTC TCTAGACCGC
    CGAGGTGTTT TTTCATTGGA TGCCCCTTCA CCATCAGTGT GCCTTCTGAT TCAGCTAGAG
    AAGGCTGCCA CTGAGGAAGG GGGAGTAACA CCCTCTGTTT ATTCTCGTAA AGAACCTGTT
    CACTTGACTG ATAAAGAGAA GCAAAAATTG CAAGTCTGGT CTCGAATCAT GCCATATAGA
    GAGTCGTTCG CATGGGCTAT GATTCCACTG TTTGAGAACA ACCAGGCTGG TGGTGCTGCC
    TCTCCCAGTA GCCCTCTAGC ACCAAGTATG TCAGGCTCAA GTTCTCAAGA TAGTATAGTG
    GAGCCTATTT CCAAGCTCAC TTTGGATGGA AAGCTCAATC ATTATTCAAG TGGAAGCTCA
    GTTATTGTAG AGATATCAAA TTTGAACAAA GTGAAGGAAA GCTACATAGA GGACTCCCTC
    CAAGACCCCA AAAGAAAAGT ACACAAACCA GTGAAAGGTG TACTGAGGTT AGAGGTGGAA
    AAACTTCATA ATGGCCACAA CGACATGGAT AACATTTCTG AGGGCGGTAG CATGGCCAAT
    GATTTGAATG ATGCTGGTGA ACTCAATAAT GGTAGATACA ACCGGAGTAG CTTTGATGGG
    ATCCATGGTT CTTTGAATTC TAGTGCCGTG GCCCAGAAAG ATGCACATCA GAATGGTCAA
    GCTTCCAATA CAGAGAGTGG TGAAAATTTT CAAGCTTTTG ACTTTCGGAT GATGACCCGA
    AGTGAGCCAT TTTCACAACT CTTCCATTGT CTCTATGTGT ACCCACTGAC TATTAGCTTG
    GGCCGTAAAA GAAATCTATT TGTAAGAGTG GAATTGAGAA AGGATGATTC TGACATCCGC
    AAGCCTCCAT TAGAGGCTGT TCATCCAAGG GATCGGAACA CAACACTACA GAAGTGGGCG
    CATACTCAGA TTGCTGTTGG AACGAGAATG GCTTGCTACC ATGATGAAGT TAAAATCAGT
    CTGCCTGCTC TCTTGACACC CCAACATCAT CTTCTGTTCA CATTTTACCA TGTAGATCTC
    CAAATGAAAC CTGAAGCCCC CAAACCAGTT GTCGTGGGAT ATGCTGTTCT TCCACTGTCG
    ACACACATTC AGTTACTTTC AGACGTATCT TTGCCAATTT TAAGAGAACT TGTTCCACAT
    TACCTGCAGG AAAGTGGAAA GGAAAGAATG GACTATCTGG AGGATGGCAA AACTGTTTTT
    AGGTTGCGAT TAAGACTTTG TTCTTCATTA TTCCCAGTTA ATGAAAGAAT AAGAGACTTT
    TTTGTCGAGT ATGACCGCCA TACACTTCAT ACAAGTCCAC CTTGGGGTTC TGAGCTTCTT
    GAGGCCATAA ACAGTTTAAA GAATGTTGAA TCTACTGCAC TGTTGCAATT TCTCCAACCC
    ATACTTAATA TGCTACTTCA TCTTATCGGT GATGGTGGTG AAACCCTTCA GGTTGCCGCA
    TTTCGAGCTA TGGTCAATAT CCTAACCCGG GTCCAGCAGG AATCATCTGA TGGGGCTGAG
    AGAAATCGGT TTCTTGTTAG TTATGTTGAT TATGCTTTTG ATGACTTTGG TGATCGGCAA
    GCACCTGTAT ACCCTGGTCT GTCTACTGTT TGGGGAAGCC TGGCTCGGAG TAAGGCTAAA
    GGTTATAGAG TTGGGCCAGT CTATGATGAT GTGCTGGCAA TGGCTTGGTT TTTTCTGGAG
    CTTATTGTAA AATCGATGGG ATTGGAACAA AGTCGTCTAT TTTACCATAA TCTTCCACTA
    GGTGAAGATG TCCCACCGCT ACAGTTGAAA GATGGTGTCT TCCGATGTAT CATGCAGCTG
    TTTGATTGCC TTCTAACTGA GGTTCATGAG CGCTGTAAAA AGGGTTTAAG CTTGGCGAAG
    CGCTTGAACA GCACACTTGC TTTCTTTTGT TATGATCTTC TATCAATTAT TGAGCCACGC
    CAAGTTTTTG AGCTGGTTTC ATTGTATATG GACAAGTTTG CAGGAGTTTG CCAGTCAATT
    CTCCATGATT GCAAATTGAC ATTCCTTCAG ATAATATGTG ACCATGATCT TTTTGTTGAG
    ATGCCTGGTC GTGACCCCTC TGATAGAAAT TATCTCTCGT CAGTACTTAT TCAAGAGATT
    TTTCTCACCC TTGACCATGA TGATCTATCT CAGCGAGCGA AAGCAGCCCG TATTTTGGTT
    GTTCTAATAT GCAAGCATGA ATTTGATGCA CGATATCAAA AAAGTGAAGA CAAGCTGTAT
    ATTGCTCAGC TGTATTTTTC TCTTATAGGA CAGATTCTAG ATGAGATGCC TGTATTCTAT
    AATCTAAATG CTGTTGAAAA GCGTGAGGTG CTGGTAGTCA TTTTGCAAAT CATAAGGAAC
    TTGGACGACA TGACTCTGAT CAAAGCATGG CAACAGAGCA TTGCTAGAAC CAGATTGTTC
    TTCAAGCTAC TTGAAGAATG CATTACCCAT TTTGAGCACA ACAAAACAGG AGACAGCTTG
    TTACTAGGCT CAAGTTCACG GAGCCCTGAT GCTGAGCGCC CTGCATCTCC AAAGTACTCC
    GATCGATTAT CCCCATCAGT TAATGCATAT TTGTCTGAGG CATCACGCCA TGAAATAAGG
    CCTCAGGGAA CACCAGAAAA TGGATACATG TGGAACAGGG TTAGTCCTCA ACTGAGCTCT
    CCTAACCAGC CCTATTCATT GAGAGAAGCA CTTGCTCAAG CGCAGTCTTC AAGGATTGGA
    TCAACCGCGA GGGCACTGAG AGAATCTCTA CATCCAGTGC TGAGACAAAA GTTGGAACTG
    TGGGAAGAAA ATCTCAGTAC TGCTGTGAGC CTGGAAGTGT TGGGAATAAT TGATAAGTTT
    TCAGTCGCTG CAGCTTCTCG TAGCATCACT ACTGATTACG CAAAGCTAGA TTGTGTAACA
    TCTGTATTAA TGGGTCTTTT ATCAAGGAGC CAGCCCTTAG CTTTTTGGAA AGCTTTCCTT
    CCTGTGGTGT ATAATATATT TAACCTCCAT GGCGCAACAC TGATGGCAAG GGAGAATGAT
    CGCTTCTTGA AGCAAATTGC TTTTCATCTT TTGCGCCTTG CTGTTTTCCG GAATGATTCT
    ATTAGAAAAA GGGCTGTTGT TGGGTTGCAG ATTCTTGTTA GGAACTCGTT TAATTATTTT
    AAGAACACCA CAAGGTTGAG GGTGATGTTG ACAATCACTT TGTCAGAACT TATGTCTGAT
    GTGCAAGTAA CTCAGATGAA ATCTGATGGC TCTCTTGAGG AAAGTGGTGA AACTCGGTGC
    CTTAGGAAAT CACTAGAGGA AATGGCTGAT GTACGAAGTA AGGATCTCTT GAAAGATTGT
    GGCCTCCCTG TTAATGCACT GGAGGCAGCG CCTGAAGGGT CGACTGATAA TAGGTGGTCT
    TGGGTAGAAG TTAAACATCT ATCCAAATGT CTAGTCCAGG CCCTTGATGC TGGTCTTGAA
    CATGCCCTCT TGGGCTCTGA AATGACTCTG GACAGGTACG CTGCTGCTGA GGGTTTCTAT
    AAGCTAGCAA TGGCCTACGC GCCTGTGCCA GATCTTCATA TAATGTGGTT ACTGCATCTA
    TGTGATGCAC ATCAGGAGAT GCAATCATGG GCTGAAGCTG CACAATGTGC AGTTGCTGTA
    GCTGGTGTTA TCATGCAGGC ACTTGTTGGA AGGAATGATG CGGTGTGGAG TAAGGAGCAT
    GTTGCTTCCC TATGTAAGAT TTGCCCTATT GTGAACACTG ATGTTAGCTC AGAAGCATCT
    GCAGCAGAAG TTGAGGGATA TGGTGCATCC AAGCTTACTG TGGATTCAGC TGTCAAGTAT
    CTTCAGCTGG CAAACAAACT TTTTGCACAA GCCGAACTCT ATCACTTTTG TGCTAGTATT
    CAGGAACTCA TCATTCCTGT GTATAAAAGC AGAAGGGCAT ATGGGCATCT GGCTAAGTGT
    CATACATCAC TCAAGGATAT CTATGAGTCA ATTCTTGAAC AGGAGGCTAG TCCTATACCA
    TTCATTGATG CTACCTACTA CCGGGTAGGG TTTTATGGGG AGCGGTTTGG CAAACTCAAC
    AAAAAGGAGT ATGTGTTCAG AGAGCCAAGA GATGTTCGTC TTGGTGACAT AATGGAGAAG
    CTTAGTCATA TCTATGAGGC TAAAATGGAT GGCAATCACA CCTTACACAT CATTCCGGAT
    TCGAGACAAG TCAACGCTGA TGAGTTGCAA CCTGGTGTCT GCTATCTGCA GATAACTGCT
    GTTGATCCTG TAATGGAGGA TGAGGACCTA GGGAGTAGAA GGGAAAGGAT TTTCTCTTTA
    TCTACTGGTA CTGTCCGGGC CCGTGTGTTC GACCGTTTTC TTTTTGATAC ACCATTCACA
    AAGAATGGAA AAACACAAGG TGGCCTTGAA GATCAGTGGA AGAGGCGCAC AGTACTCCAG
    ACAGAGGGTT CCTTTCCTGC TTTGGTGAAT CGTCTGTTAG TGATCAAATC AGAATCTCTA
    GAGTTTTCTC CTGTTGAGAA TGCGATTGGA ATGATCGAGA CAAGGACAGC TGCCTTGCGA
    AATGAACTAG AAGAACCACG CAGTTCGGAA GGTGACCAAC TCCCAAGACT CCAAAGCCTG
    CAGAGGATAC TCCAAGGAAG TGTGGCTGTT CAGGTAAATA GTGGTGTATT AAGTGTGTGC
    ACTGCATTCT TATCTGGTGA GCCCGCAACT AGGCTCCGAT CTCAAGAACT GCAGCAGCTC
    ATCGCTGCAT TGCTGGAATT CATGGCCGTC TGCAAGCGCG CAATCCGTGT GCATTTTAGA
    TTGATAGGGG AAGAAGACCA GGAATTCCAC ACTCAACTAG TCAATGGCTT TCAGTCGCTC
    ACAGCTGAGC TTTCACACTA TATTCCTGCA ATTCTTTCAG AGCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015631338.1
    CDS318..5825
    Translation

    Target ORF information:

    RefSeq Version XM_015775852.1
    Organism Oryza sativa Japonica Group(Japanese rice)
    Definition Oryza sativa Japonica Group guanine nucleotide exchange factor SPIKE 1 (LOC4332718), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015775852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATTCCG CGGCGGCGGC GGGGGAGGGG CAGCGGTTCA AGCGCATTCC GCGGCAGTCG 
    TTGGCGGGGA ACCTGGAGCT GGATCCACTG CTTAATGAAA ATTTAGATCA ATGGCCACAT
    CTAAATGAGC TGGTTCAGTG CTATAAGGCT GATTTTGTGA AAGACGACTG CAAATATGGA
    CGATATGAAA GTGTTGCACC ACCATCTTTT CAGAATCAAA TTTTTGAGGG ACCTGATACT
    GACTTAGAAA CAGAATTGCA GCTTAGCAAT GATAGGCAAT CCAAGCCTGA TGAAGTTACT
    GAAGATGATA TGCCAAGCAC ATCAGGGAGA CAATTATATG AGACTGAAGT GCCCGCTTCA
    TCCTCAAAAA AACACTGCAG TCTATCACCT TTACCAGCAT ATGAACCGGC ATTTGATTGG
    GAAAATGAGA GATCTTTGAT ATTCGGCCAA CGGGTGCCAG AAAGTGTCCC TGCAATAAAC
    AGCAGTGGAT TGAAGATAAC TGTCAAGGTG TTATCTTTGT CATTTCAAGC TGGATTAGTT
    GAGCCTTTTA GTGGTACAAT CTGCTTGTAC AACAGAGACA GAAGAGAAAA ATTGTCAGAA
    GATTTCTATT TTCATATACT CCCAACAGAA ATGCAAGATG CTCAGATTTC TCTAGACCGC
    CGAGGTGTTT TTTCATTGGA TGCCCCTTCA CCATCAGTGT GCCTTCTGAT TCAGCTAGAG
    AAGGCTGCCA CTGAGGAAGG GGGAGTAACA CCCTCTGTTT ATTCTCGTAA AGAACCTGTT
    CACTTGACTG ATAAAGAGAA GCAAAAATTG CAAGTCTGGT CTCGAATCAT GCCATATAGA
    GAGTCGTTCG CATGGGCTAT GATTCCACTG TTTGAGAACA ACCAGGCTGG TGGTGCTGCC
    TCTCCCAGTA GCCCTCTAGC ACCAAGTATG TCAGGCTCAA GTTCTCAAGA TAGTATAGTG
    GAGCCTATTT CCAAGCTCAC TTTGGATGGA AAGCTCAATC ATTATTCAAG TGGAAGCTCA
    GTTATTGTAG AGATATCAAA TTTGAACAAA GTGAAGGAAA GCTACATAGA GGACTCCCTC
    CAAGACCCCA AAAGAAAAGT ACACAAACCA GTGAAAGGTG TACTGAGGTT AGAGGTGGAA
    AAACTTCATA ATGGCCACAA CGACATGGAT AACATTTCTG AGGGCGGTAG CATGGCCAAT
    GATTTGAATG ATGCTGGTGA ACTCAATAAT GGTAGATACA ACCGGAGTAG CTTTGATGGG
    ATCCATGGTT CTTTGAATTC TAGTGCCGTG GCCCAGAAAG ATGCACATCA GAATGGTCAA
    GCTTCCAATA CAGAGAGTGG TGAAAATTTT CAAGCTTTTG ACTTTCGGAT GATGACCCGA
    AGTGAGCCAT TTTCACAACT CTTCCATTGT CTCTATGTGT ACCCACTGAC TATTAGCTTG
    GGCCGTAAAA GAAATCTATT TGTAAGAGTG GAATTGAGAA AGGATGATTC TGACATCCGC
    AAGCCTCCAT TAGAGGCTGT TCATCCAAGG GATCGGAACA CAACACTACA GAAGTGGGCG
    CATACTCAGA TTGCTGTTGG AACGAGAATG GCTTGCTACC ATGATGAAGT TAAAATCAGT
    CTGCCTGCTC TCTTGACACC CCAACATCAT CTTCTGTTCA CATTTTACCA TGTAGATCTC
    CAAATGAAAC CTGAAGCCCC CAAACCAGTT GTCGTGGGAT ATGCTGTTCT TCCACTGTCG
    ACACACATTC AGTTACTTTC AGACGTATCT TTGCCAATTT TAAGAGAACT TGTTCCACAT
    TACCTGCAGG AAAGTGGAAA GGAAAGAATG GACTATCTGG AGGATGGCAA AACTGTTTTT
    AGGTTGCGAT TAAGACTTTG TTCTTCATTA TTCCCAGTTA ATGAAAGAAT AAGAGACTTT
    TTTGTCGAGT ATGACCGCCA TACACTTCAT ACAAGTCCAC CTTGGGGTTC TGAGCTTCTT
    GAGGCCATAA ACAGTTTAAA GAATGTTGAA TCTACTGCAC TGTTGCAATT TCTCCAACCC
    ATACTTAATA TGCTACTTCA TCTTATCGGT GATGGTGGTG AAACCCTTCA GGTTGCCGCA
    TTTCGAGCTA TGGTCAATAT CCTAACCCGG GTCCAGCAGG AATCATCTGA TGGGGCTGAG
    AGAAATCGGT TTCTTGTTAG TTATGTTGAT TATGCTTTTG ATGACTTTGG TGATCGGCAA
    GCACCTGTAT ACCCTGGTCT GTCTACTGTT TGGGGAAGCC TGGCTCGGAG TAAGGCTAAA
    GGTTATAGAG TTGGGCCAGT CTATGATGAT GTGCTGGCAA TGGCTTGGTT TTTTCTGGAG
    CTTATTGTAA AATCGATGGG ATTGGAACAA AGTCGTCTAT TTTACCATAA TCTTCCACTA
    GGTGAAGATG TCCCACCGCT ACAGTTGAAA GATGGTGTCT TCCGATGTAT CATGCAGCTG
    TTTGATTGCC TTCTAACTGA GGTTCATGAG CGCTGTAAAA AGGGTTTAAG CTTGGCGAAG
    CGCTTGAACA GCACACTTGC TTTCTTTTGT TATGATCTTC TATCAATTAT TGAGCCACGC
    CAAGTTTTTG AGCTGGTTTC ATTGTATATG GACAAGTTTG CAGGAGTTTG CCAGTCAATT
    CTCCATGATT GCAAATTGAC ATTCCTTCAG ATAATATGTG ACCATGATCT TTTTGTTGAG
    ATGCCTGGTC GTGACCCCTC TGATAGAAAT TATCTCTCGT CAGTACTTAT TCAAGAGATT
    TTTCTCACCC TTGACCATGA TGATCTATCT CAGCGAGCGA AAGCAGCCCG TATTTTGGTT
    GTTCTAATAT GCAAGCATGA ATTTGATGCA CGATATCAAA AAAGTGAAGA CAAGCTGTAT
    ATTGCTCAGC TGTATTTTTC TCTTATAGGA CAGATTCTAG ATGAGATGCC TGTATTCTAT
    AATCTAAATG CTGTTGAAAA GCGTGAGGTG CTGGTAGTCA TTTTGCAAAT CATAAGGAAC
    TTGGACGACA TGACTCTGAT CAAAGCATGG CAACAGAGCA TTGCTAGAAC CAGATTGTTC
    TTCAAGCTAC TTGAAGAATG CATTACCCAT TTTGAGCACA ACAAAACAGG AGACAGCTTG
    TTACTAGGCT CAAGTTCACG GAGCCCTGAT GCTGAGCGCC CTGCATCTCC AAAGTACTCC
    GATCGATTAT CCCCATCAGT TAATGCATAT TTGTCTGAGG CATCACGCCA TGAAATAAGG
    CCTCAGGGAA CACCAGAAAA TGGATACATG TGGAACAGGG TTAGTCCTCA ACTGAGCTCT
    CCTAACCAGC CCTATTCATT GAGAGAAGCA CTTGCTCAAG CGCAGTCTTC AAGGATTGGA
    TCAACCGCGA GGGCACTGAG AGAATCTCTA CATCCAGTGC TGAGACAAAA GTTGGAACTG
    TGGGAAGAAA ATCTCAGTAC TGCTGTGAGC CTGGAAGTGT TGGGAATAAT TGATAAGTTT
    TCAGTCGCTG CAGCTTCTCG TAGCATCACT ACTGATTACG CAAAGCTAGA TTGTGTAACA
    TCTGTATTAA TGGGTCTTTT ATCAAGGAGC CAGCCCTTAG CTTTTTGGAA AGCTTTCCTT
    CCTGTGGTGT ATAATATATT TAACCTCCAT GGCGCAACAC TGATGGCAAG GGAGAATGAT
    CGCTTCTTGA AGCAAATTGC TTTTCATCTT TTGCGCCTTG CTGTTTTCCG GAATGATTCT
    ATTAGAAAAA GGGCTGTTGT TGGGTTGCAG ATTCTTGTTA GGAACTCGTT TAATTATTTT
    AAGAACACCA CAAGGTTGAG GGTGATGTTG ACAATCACTT TGTCAGAACT TATGTCTGAT
    GTGCAAGTAA CTCAGATGAA ATCTGATGGC TCTCTTGAGG AAAGTGGTGA AACTCGGTGC
    CTTAGGAAAT CACTAGAGGA AATGGCTGAT GTACGAAGTA AGGATCTCTT GAAAGATTGT
    GGCCTCCCTG TTAATGCACT GGAGGCAGCG CCTGAAGGGT CGACTGATAA TAGGTGGTCT
    TGGGTAGAAG TTAAACATCT ATCCAAATGT CTAGTCCAGG CCCTTGATGC TGGTCTTGAA
    CATGCCCTCT TGGGCTCTGA AATGACTCTG GACAGGTACG CTGCTGCTGA GGGTTTCTAT
    AAGCTAGCAA TGGCCTACGC GCCTGTGCCA GATCTTCATA TAATGTGGTT ACTGCATCTA
    TGTGATGCAC ATCAGGAGAT GCAATCATGG GCTGAAGCTG CACAATGTGC AGTTGCTGTA
    GCTGGTGTTA TCATGCAGGC ACTTGTTGGA AGGAATGATG CGGTGTGGAG TAAGGAGCAT
    GTTGCTTCCC TATGTAAGAT TTGCCCTATT GTGAACACTG ATGTTAGCTC AGAAGCATCT
    GCAGCAGAAG TTGAGGGATA TGGTGCATCC AAGCTTACTG TGGATTCAGC TGTCAAGTAT
    CTTCAGCTGG CAAACAAACT TTTTGCACAA GCCGAACTCT ATCACTTTTG TGCTAGTATT
    CAGGAACTCA TCATTCCTGT GTATAAAAGC AGAAGGGCAT ATGGGCATCT GGCTAAGTGT
    CATACATCAC TCAAGGATAT CTATGAGTCA ATTCTTGAAC AGGAGGCTAG TCCTATACCA
    TTCATTGATG CTACCTACTA CCGGGTAGGG TTTTATGGGG AGCGGTTTGG CAAACTCAAC
    AAAAAGGAGT ATGTGTTCAG AGAGCCAAGA GATGTTCGTC TTGGTGACAT AATGGAGAAG
    CTTAGTCATA TCTATGAGGC TAAAATGGAT GGCAATCACA CCTTACACAT CATTCCGGAT
    TCGAGACAAG TCAACGCTGA TGAGTTGCAA CCTGGTGTCT GCTATCTGCA GATAACTGCT
    GTTGATCCTG TAATGGAGGA TGAGGACCTA GGGAGTAGAA GGGAAAGGAT TTTCTCTTTA
    TCTACTGGTA CTGTCCGGGC CCGTGTGTTC GACCGTTTTC TTTTTGATAC ACCATTCACA
    AAGAATGGAA AAACACAAGG TGGCCTTGAA GATCAGTGGA AGAGGCGCAC AGTACTCCAG
    ACAGAGGGTT CCTTTCCTGC TTTGGTGAAT CGTCTGTTAG TGATCAAATC AGAATCTCTA
    GAGTTTTCTC CTGTTGAGAA TGCGATTGGA ATGATCGAGA CAAGGACAGC TGCCTTGCGA
    AATGAACTAG AAGAACCACG CAGTTCGGAA GGTGACCAAC TCCCAAGACT CCAAAGCCTG
    CAGAGGATAC TCCAAGGAAG TGTGGCTGTT CAGGTAAATA GTGGTGTATT AAGTGTGTGC
    ACTGCATTCT TATCTGGTGA GCCCGCAACT AGGCTCCGAT CTCAAGAACT GCAGCAGCTC
    ATCGCTGCAT TGCTGGAATT CATGGCCGTC TGCAAGCGCG CAATCCGTGT GCATTTTAGA
    TTGATAGGGG AAGAAGACCA GGAATTCCAC ACTCAACTAG TCAATGGCTT TCAGTCGCTC
    ACAGCTGAGC TTTCACACTA TATTCCTGCA ATTCTTTCAG AGCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOa30426
    Clone ID Related Accession (Same CDS sequence) XM_015775852.1 , XM_015775852.2 , XM_015775853.1
    Accession Version XM_015775852.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-08-06
    Organism Oryza sativa Japonica Group(Japanese rice)
    Product guanine nucleotide exchange factor SPIKE 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015775852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATTCCG CGGCGGCGGC GGGGGAGGGG CAGCGGTTCA AGCGCATTCC GCGGCAGTCG 
    TTGGCGGGGA ACCTGGAGCT GGATCCACTG CTTAATGAAA ATTTAGATCA ATGGCCACAT
    CTAAATGAGC TGGTTCAGTG CTATAAGGCT GATTTTGTGA AAGACGACTG CAAATATGGA
    CGATATGAAA GTGTTGCACC ACCATCTTTT CAGAATCAAA TTTTTGAGGG ACCTGATACT
    GACTTAGAAA CAGAATTGCA GCTTAGCAAT GATAGGCAAT CCAAGCCTGA TGAAGTTACT
    GAAGATGATA TGCCAAGCAC ATCAGGGAGA CAATTATATG AGACTGAAGT GCCCGCTTCA
    TCCTCAAAAA AACACTGCAG TCTATCACCT TTACCAGCAT ATGAACCGGC ATTTGATTGG
    GAAAATGAGA GATCTTTGAT ATTCGGCCAA CGGGTGCCAG AAAGTGTCCC TGCAATAAAC
    AGCAGTGGAT TGAAGATAAC TGTCAAGGTG TTATCTTTGT CATTTCAAGC TGGATTAGTT
    GAGCCTTTTA GTGGTACAAT CTGCTTGTAC AACAGAGACA GAAGAGAAAA ATTGTCAGAA
    GATTTCTATT TTCATATACT CCCAACAGAA ATGCAAGATG CTCAGATTTC TCTAGACCGC
    CGAGGTGTTT TTTCATTGGA TGCCCCTTCA CCATCAGTGT GCCTTCTGAT TCAGCTAGAG
    AAGGCTGCCA CTGAGGAAGG GGGAGTAACA CCCTCTGTTT ATTCTCGTAA AGAACCTGTT
    CACTTGACTG ATAAAGAGAA GCAAAAATTG CAAGTCTGGT CTCGAATCAT GCCATATAGA
    GAGTCGTTCG CATGGGCTAT GATTCCACTG TTTGAGAACA ACCAGGCTGG TGGTGCTGCC
    TCTCCCAGTA GCCCTCTAGC ACCAAGTATG TCAGGCTCAA GTTCTCAAGA TAGTATAGTG
    GAGCCTATTT CCAAGCTCAC TTTGGATGGA AAGCTCAATC ATTATTCAAG TGGAAGCTCA
    GTTATTGTAG AGATATCAAA TTTGAACAAA GTGAAGGAAA GCTACATAGA GGACTCCCTC
    CAAGACCCCA AAAGAAAAGT ACACAAACCA GTGAAAGGTG TACTGAGGTT AGAGGTGGAA
    AAACTTCATA ATGGCCACAA CGACATGGAT AACATTTCTG AGGGCGGTAG CATGGCCAAT
    GATTTGAATG ATGCTGGTGA ACTCAATAAT GGTAGATACA ACCGGAGTAG CTTTGATGGG
    ATCCATGGTT CTTTGAATTC TAGTGCCGTG GCCCAGAAAG ATGCACATCA GAATGGTCAA
    GCTTCCAATA CAGAGAGTGG TGAAAATTTT CAAGCTTTTG ACTTTCGGAT GATGACCCGA
    AGTGAGCCAT TTTCACAACT CTTCCATTGT CTCTATGTGT ACCCACTGAC TATTAGCTTG
    GGCCGTAAAA GAAATCTATT TGTAAGAGTG GAATTGAGAA AGGATGATTC TGACATCCGC
    AAGCCTCCAT TAGAGGCTGT TCATCCAAGG GATCGGAACA CAACACTACA GAAGTGGGCG
    CATACTCAGA TTGCTGTTGG AACGAGAATG GCTTGCTACC ATGATGAAGT TAAAATCAGT
    CTGCCTGCTC TCTTGACACC CCAACATCAT CTTCTGTTCA CATTTTACCA TGTAGATCTC
    CAAATGAAAC CTGAAGCCCC CAAACCAGTT GTCGTGGGAT ATGCTGTTCT TCCACTGTCG
    ACACACATTC AGTTACTTTC AGACGTATCT TTGCCAATTT TAAGAGAACT TGTTCCACAT
    TACCTGCAGG AAAGTGGAAA GGAAAGAATG GACTATCTGG AGGATGGCAA AACTGTTTTT
    AGGTTGCGAT TAAGACTTTG TTCTTCATTA TTCCCAGTTA ATGAAAGAAT AAGAGACTTT
    TTTGTCGAGT ATGACCGCCA TACACTTCAT ACAAGTCCAC CTTGGGGTTC TGAGCTTCTT
    GAGGCCATAA ACAGTTTAAA GAATGTTGAA TCTACTGCAC TGTTGCAATT TCTCCAACCC
    ATACTTAATA TGCTACTTCA TCTTATCGGT GATGGTGGTG AAACCCTTCA GGTTGCCGCA
    TTTCGAGCTA TGGTCAATAT CCTAACCCGG GTCCAGCAGG AATCATCTGA TGGGGCTGAG
    AGAAATCGGT TTCTTGTTAG TTATGTTGAT TATGCTTTTG ATGACTTTGG TGATCGGCAA
    GCACCTGTAT ACCCTGGTCT GTCTACTGTT TGGGGAAGCC TGGCTCGGAG TAAGGCTAAA
    GGTTATAGAG TTGGGCCAGT CTATGATGAT GTGCTGGCAA TGGCTTGGTT TTTTCTGGAG
    CTTATTGTAA AATCGATGGG ATTGGAACAA AGTCGTCTAT TTTACCATAA TCTTCCACTA
    GGTGAAGATG TCCCACCGCT ACAGTTGAAA GATGGTGTCT TCCGATGTAT CATGCAGCTG
    TTTGATTGCC TTCTAACTGA GGTTCATGAG CGCTGTAAAA AGGGTTTAAG CTTGGCGAAG
    CGCTTGAACA GCACACTTGC TTTCTTTTGT TATGATCTTC TATCAATTAT TGAGCCACGC
    CAAGTTTTTG AGCTGGTTTC ATTGTATATG GACAAGTTTG CAGGAGTTTG CCAGTCAATT
    CTCCATGATT GCAAATTGAC ATTCCTTCAG ATAATATGTG ACCATGATCT TTTTGTTGAG
    ATGCCTGGTC GTGACCCCTC TGATAGAAAT TATCTCTCGT CAGTACTTAT TCAAGAGATT
    TTTCTCACCC TTGACCATGA TGATCTATCT CAGCGAGCGA AAGCAGCCCG TATTTTGGTT
    GTTCTAATAT GCAAGCATGA ATTTGATGCA CGATATCAAA AAAGTGAAGA CAAGCTGTAT
    ATTGCTCAGC TGTATTTTTC TCTTATAGGA CAGATTCTAG ATGAGATGCC TGTATTCTAT
    AATCTAAATG CTGTTGAAAA GCGTGAGGTG CTGGTAGTCA TTTTGCAAAT CATAAGGAAC
    TTGGACGACA TGACTCTGAT CAAAGCATGG CAACAGAGCA TTGCTAGAAC CAGATTGTTC
    TTCAAGCTAC TTGAAGAATG CATTACCCAT TTTGAGCACA ACAAAACAGG AGACAGCTTG
    TTACTAGGCT CAAGTTCACG GAGCCCTGAT GCTGAGCGCC CTGCATCTCC AAAGTACTCC
    GATCGATTAT CCCCATCAGT TAATGCATAT TTGTCTGAGG CATCACGCCA TGAAATAAGG
    CCTCAGGGAA CACCAGAAAA TGGATACATG TGGAACAGGG TTAGTCCTCA ACTGAGCTCT
    CCTAACCAGC CCTATTCATT GAGAGAAGCA CTTGCTCAAG CGCAGTCTTC AAGGATTGGA
    TCAACCGCGA GGGCACTGAG AGAATCTCTA CATCCAGTGC TGAGACAAAA GTTGGAACTG
    TGGGAAGAAA ATCTCAGTAC TGCTGTGAGC CTGGAAGTGT TGGGAATAAT TGATAAGTTT
    TCAGTCGCTG CAGCTTCTCG TAGCATCACT ACTGATTACG CAAAGCTAGA TTGTGTAACA
    TCTGTATTAA TGGGTCTTTT ATCAAGGAGC CAGCCCTTAG CTTTTTGGAA AGCTTTCCTT
    CCTGTGGTGT ATAATATATT TAACCTCCAT GGCGCAACAC TGATGGCAAG GGAGAATGAT
    CGCTTCTTGA AGCAAATTGC TTTTCATCTT TTGCGCCTTG CTGTTTTCCG GAATGATTCT
    ATTAGAAAAA GGGCTGTTGT TGGGTTGCAG ATTCTTGTTA GGAACTCGTT TAATTATTTT
    AAGAACACCA CAAGGTTGAG GGTGATGTTG ACAATCACTT TGTCAGAACT TATGTCTGAT
    GTGCAAGTAA CTCAGATGAA ATCTGATGGC TCTCTTGAGG AAAGTGGTGA AACTCGGTGC
    CTTAGGAAAT CACTAGAGGA AATGGCTGAT GTACGAAGTA AGGATCTCTT GAAAGATTGT
    GGCCTCCCTG TTAATGCACT GGAGGCAGCG CCTGAAGGGT CGACTGATAA TAGGTGGTCT
    TGGGTAGAAG TTAAACATCT ATCCAAATGT CTAGTCCAGG CCCTTGATGC TGGTCTTGAA
    CATGCCCTCT TGGGCTCTGA AATGACTCTG GACAGGTACG CTGCTGCTGA GGGTTTCTAT
    AAGCTAGCAA TGGCCTACGC GCCTGTGCCA GATCTTCATA TAATGTGGTT ACTGCATCTA
    TGTGATGCAC ATCAGGAGAT GCAATCATGG GCTGAAGCTG CACAATGTGC AGTTGCTGTA
    GCTGGTGTTA TCATGCAGGC ACTTGTTGGA AGGAATGATG CGGTGTGGAG TAAGGAGCAT
    GTTGCTTCCC TATGTAAGAT TTGCCCTATT GTGAACACTG ATGTTAGCTC AGAAGCATCT
    GCAGCAGAAG TTGAGGGATA TGGTGCATCC AAGCTTACTG TGGATTCAGC TGTCAAGTAT
    CTTCAGCTGG CAAACAAACT TTTTGCACAA GCCGAACTCT ATCACTTTTG TGCTAGTATT
    CAGGAACTCA TCATTCCTGT GTATAAAAGC AGAAGGGCAT ATGGGCATCT GGCTAAGTGT
    CATACATCAC TCAAGGATAT CTATGAGTCA ATTCTTGAAC AGGAGGCTAG TCCTATACCA
    TTCATTGATG CTACCTACTA CCGGGTAGGG TTTTATGGGG AGCGGTTTGG CAAACTCAAC
    AAAAAGGAGT ATGTGTTCAG AGAGCCAAGA GATGTTCGTC TTGGTGACAT AATGGAGAAG
    CTTAGTCATA TCTATGAGGC TAAAATGGAT GGCAATCACA CCTTACACAT CATTCCGGAT
    TCGAGACAAG TCAACGCTGA TGAGTTGCAA CCTGGTGTCT GCTATCTGCA GATAACTGCT
    GTTGATCCTG TAATGGAGGA TGAGGACCTA GGGAGTAGAA GGGAAAGGAT TTTCTCTTTA
    TCTACTGGTA CTGTCCGGGC CCGTGTGTTC GACCGTTTTC TTTTTGATAC ACCATTCACA
    AAGAATGGAA AAACACAAGG TGGCCTTGAA GATCAGTGGA AGAGGCGCAC AGTACTCCAG
    ACAGAGGGTT CCTTTCCTGC TTTGGTGAAT CGTCTGTTAG TGATCAAATC AGAATCTCTA
    GAGTTTTCTC CTGTTGAGAA TGCGATTGGA ATGATCGAGA CAAGGACAGC TGCCTTGCGA
    AATGAACTAG AAGAACCACG CAGTTCGGAA GGTGACCAAC TCCCAAGACT CCAAAGCCTG
    CAGAGGATAC TCCAAGGAAG TGTGGCTGTT CAGGTAAATA GTGGTGTATT AAGTGTGTGC
    ACTGCATTCT TATCTGGTGA GCCCGCAACT AGGCTCCGAT CTCAAGAACT GCAGCAGCTC
    ATCGCTGCAT TGCTGGAATT CATGGCCGTC TGCAAGCGCG CAATCCGTGT GCATTTTAGA
    TTGATAGGGG AAGAAGACCA GGAATTCCAC ACTCAACTAG TCAATGGCTT TCAGTCGCTC
    ACAGCTGAGC TTTCACACTA TATTCCTGCA ATTCTTTCAG AGCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015631338.1
    CDS329..5836
    Translation

    Target ORF information:

    RefSeq Version XM_015775852.2
    Organism Oryza sativa Japonica Group(Japanese rice)
    Definition Oryza sativa Japonica Group guanine nucleotide exchange factor SPIKE 1 (LOC4332718), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015775852.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATTCCG CGGCGGCGGC GGGGGAGGGG CAGCGGTTCA AGCGCATTCC GCGGCAGTCG 
    TTGGCGGGGA ACCTGGAGCT GGATCCACTG CTTAATGAAA ATTTAGATCA ATGGCCACAT
    CTAAATGAGC TGGTTCAGTG CTATAAGGCT GATTTTGTGA AAGACGACTG CAAATATGGA
    CGATATGAAA GTGTTGCACC ACCATCTTTT CAGAATCAAA TTTTTGAGGG ACCTGATACT
    GACTTAGAAA CAGAATTGCA GCTTAGCAAT GATAGGCAAT CCAAGCCTGA TGAAGTTACT
    GAAGATGATA TGCCAAGCAC ATCAGGGAGA CAATTATATG AGACTGAAGT GCCCGCTTCA
    TCCTCAAAAA AACACTGCAG TCTATCACCT TTACCAGCAT ATGAACCGGC ATTTGATTGG
    GAAAATGAGA GATCTTTGAT ATTCGGCCAA CGGGTGCCAG AAAGTGTCCC TGCAATAAAC
    AGCAGTGGAT TGAAGATAAC TGTCAAGGTG TTATCTTTGT CATTTCAAGC TGGATTAGTT
    GAGCCTTTTA GTGGTACAAT CTGCTTGTAC AACAGAGACA GAAGAGAAAA ATTGTCAGAA
    GATTTCTATT TTCATATACT CCCAACAGAA ATGCAAGATG CTCAGATTTC TCTAGACCGC
    CGAGGTGTTT TTTCATTGGA TGCCCCTTCA CCATCAGTGT GCCTTCTGAT TCAGCTAGAG
    AAGGCTGCCA CTGAGGAAGG GGGAGTAACA CCCTCTGTTT ATTCTCGTAA AGAACCTGTT
    CACTTGACTG ATAAAGAGAA GCAAAAATTG CAAGTCTGGT CTCGAATCAT GCCATATAGA
    GAGTCGTTCG CATGGGCTAT GATTCCACTG TTTGAGAACA ACCAGGCTGG TGGTGCTGCC
    TCTCCCAGTA GCCCTCTAGC ACCAAGTATG TCAGGCTCAA GTTCTCAAGA TAGTATAGTG
    GAGCCTATTT CCAAGCTCAC TTTGGATGGA AAGCTCAATC ATTATTCAAG TGGAAGCTCA
    GTTATTGTAG AGATATCAAA TTTGAACAAA GTGAAGGAAA GCTACATAGA GGACTCCCTC
    CAAGACCCCA AAAGAAAAGT ACACAAACCA GTGAAAGGTG TACTGAGGTT AGAGGTGGAA
    AAACTTCATA ATGGCCACAA CGACATGGAT AACATTTCTG AGGGCGGTAG CATGGCCAAT
    GATTTGAATG ATGCTGGTGA ACTCAATAAT GGTAGATACA ACCGGAGTAG CTTTGATGGG
    ATCCATGGTT CTTTGAATTC TAGTGCCGTG GCCCAGAAAG ATGCACATCA GAATGGTCAA
    GCTTCCAATA CAGAGAGTGG TGAAAATTTT CAAGCTTTTG ACTTTCGGAT GATGACCCGA
    AGTGAGCCAT TTTCACAACT CTTCCATTGT CTCTATGTGT ACCCACTGAC TATTAGCTTG
    GGCCGTAAAA GAAATCTATT TGTAAGAGTG GAATTGAGAA AGGATGATTC TGACATCCGC
    AAGCCTCCAT TAGAGGCTGT TCATCCAAGG GATCGGAACA CAACACTACA GAAGTGGGCG
    CATACTCAGA TTGCTGTTGG AACGAGAATG GCTTGCTACC ATGATGAAGT TAAAATCAGT
    CTGCCTGCTC TCTTGACACC CCAACATCAT CTTCTGTTCA CATTTTACCA TGTAGATCTC
    CAAATGAAAC CTGAAGCCCC CAAACCAGTT GTCGTGGGAT ATGCTGTTCT TCCACTGTCG
    ACACACATTC AGTTACTTTC AGACGTATCT TTGCCAATTT TAAGAGAACT TGTTCCACAT
    TACCTGCAGG AAAGTGGAAA GGAAAGAATG GACTATCTGG AGGATGGCAA AACTGTTTTT
    AGGTTGCGAT TAAGACTTTG TTCTTCATTA TTCCCAGTTA ATGAAAGAAT AAGAGACTTT
    TTTGTCGAGT ATGACCGCCA TACACTTCAT ACAAGTCCAC CTTGGGGTTC TGAGCTTCTT
    GAGGCCATAA ACAGTTTAAA GAATGTTGAA TCTACTGCAC TGTTGCAATT TCTCCAACCC
    ATACTTAATA TGCTACTTCA TCTTATCGGT GATGGTGGTG AAACCCTTCA GGTTGCCGCA
    TTTCGAGCTA TGGTCAATAT CCTAACCCGG GTCCAGCAGG AATCATCTGA TGGGGCTGAG
    AGAAATCGGT TTCTTGTTAG TTATGTTGAT TATGCTTTTG ATGACTTTGG TGATCGGCAA
    GCACCTGTAT ACCCTGGTCT GTCTACTGTT TGGGGAAGCC TGGCTCGGAG TAAGGCTAAA
    GGTTATAGAG TTGGGCCAGT CTATGATGAT GTGCTGGCAA TGGCTTGGTT TTTTCTGGAG
    CTTATTGTAA AATCGATGGG ATTGGAACAA AGTCGTCTAT TTTACCATAA TCTTCCACTA
    GGTGAAGATG TCCCACCGCT ACAGTTGAAA GATGGTGTCT TCCGATGTAT CATGCAGCTG
    TTTGATTGCC TTCTAACTGA GGTTCATGAG CGCTGTAAAA AGGGTTTAAG CTTGGCGAAG
    CGCTTGAACA GCACACTTGC TTTCTTTTGT TATGATCTTC TATCAATTAT TGAGCCACGC
    CAAGTTTTTG AGCTGGTTTC ATTGTATATG GACAAGTTTG CAGGAGTTTG CCAGTCAATT
    CTCCATGATT GCAAATTGAC ATTCCTTCAG ATAATATGTG ACCATGATCT TTTTGTTGAG
    ATGCCTGGTC GTGACCCCTC TGATAGAAAT TATCTCTCGT CAGTACTTAT TCAAGAGATT
    TTTCTCACCC TTGACCATGA TGATCTATCT CAGCGAGCGA AAGCAGCCCG TATTTTGGTT
    GTTCTAATAT GCAAGCATGA ATTTGATGCA CGATATCAAA AAAGTGAAGA CAAGCTGTAT
    ATTGCTCAGC TGTATTTTTC TCTTATAGGA CAGATTCTAG ATGAGATGCC TGTATTCTAT
    AATCTAAATG CTGTTGAAAA GCGTGAGGTG CTGGTAGTCA TTTTGCAAAT CATAAGGAAC
    TTGGACGACA TGACTCTGAT CAAAGCATGG CAACAGAGCA TTGCTAGAAC CAGATTGTTC
    TTCAAGCTAC TTGAAGAATG CATTACCCAT TTTGAGCACA ACAAAACAGG AGACAGCTTG
    TTACTAGGCT CAAGTTCACG GAGCCCTGAT GCTGAGCGCC CTGCATCTCC AAAGTACTCC
    GATCGATTAT CCCCATCAGT TAATGCATAT TTGTCTGAGG CATCACGCCA TGAAATAAGG
    CCTCAGGGAA CACCAGAAAA TGGATACATG TGGAACAGGG TTAGTCCTCA ACTGAGCTCT
    CCTAACCAGC CCTATTCATT GAGAGAAGCA CTTGCTCAAG CGCAGTCTTC AAGGATTGGA
    TCAACCGCGA GGGCACTGAG AGAATCTCTA CATCCAGTGC TGAGACAAAA GTTGGAACTG
    TGGGAAGAAA ATCTCAGTAC TGCTGTGAGC CTGGAAGTGT TGGGAATAAT TGATAAGTTT
    TCAGTCGCTG CAGCTTCTCG TAGCATCACT ACTGATTACG CAAAGCTAGA TTGTGTAACA
    TCTGTATTAA TGGGTCTTTT ATCAAGGAGC CAGCCCTTAG CTTTTTGGAA AGCTTTCCTT
    CCTGTGGTGT ATAATATATT TAACCTCCAT GGCGCAACAC TGATGGCAAG GGAGAATGAT
    CGCTTCTTGA AGCAAATTGC TTTTCATCTT TTGCGCCTTG CTGTTTTCCG GAATGATTCT
    ATTAGAAAAA GGGCTGTTGT TGGGTTGCAG ATTCTTGTTA GGAACTCGTT TAATTATTTT
    AAGAACACCA CAAGGTTGAG GGTGATGTTG ACAATCACTT TGTCAGAACT TATGTCTGAT
    GTGCAAGTAA CTCAGATGAA ATCTGATGGC TCTCTTGAGG AAAGTGGTGA AACTCGGTGC
    CTTAGGAAAT CACTAGAGGA AATGGCTGAT GTACGAAGTA AGGATCTCTT GAAAGATTGT
    GGCCTCCCTG TTAATGCACT GGAGGCAGCG CCTGAAGGGT CGACTGATAA TAGGTGGTCT
    TGGGTAGAAG TTAAACATCT ATCCAAATGT CTAGTCCAGG CCCTTGATGC TGGTCTTGAA
    CATGCCCTCT TGGGCTCTGA AATGACTCTG GACAGGTACG CTGCTGCTGA GGGTTTCTAT
    AAGCTAGCAA TGGCCTACGC GCCTGTGCCA GATCTTCATA TAATGTGGTT ACTGCATCTA
    TGTGATGCAC ATCAGGAGAT GCAATCATGG GCTGAAGCTG CACAATGTGC AGTTGCTGTA
    GCTGGTGTTA TCATGCAGGC ACTTGTTGGA AGGAATGATG CGGTGTGGAG TAAGGAGCAT
    GTTGCTTCCC TATGTAAGAT TTGCCCTATT GTGAACACTG ATGTTAGCTC AGAAGCATCT
    GCAGCAGAAG TTGAGGGATA TGGTGCATCC AAGCTTACTG TGGATTCAGC TGTCAAGTAT
    CTTCAGCTGG CAAACAAACT TTTTGCACAA GCCGAACTCT ATCACTTTTG TGCTAGTATT
    CAGGAACTCA TCATTCCTGT GTATAAAAGC AGAAGGGCAT ATGGGCATCT GGCTAAGTGT
    CATACATCAC TCAAGGATAT CTATGAGTCA ATTCTTGAAC AGGAGGCTAG TCCTATACCA
    TTCATTGATG CTACCTACTA CCGGGTAGGG TTTTATGGGG AGCGGTTTGG CAAACTCAAC
    AAAAAGGAGT ATGTGTTCAG AGAGCCAAGA GATGTTCGTC TTGGTGACAT AATGGAGAAG
    CTTAGTCATA TCTATGAGGC TAAAATGGAT GGCAATCACA CCTTACACAT CATTCCGGAT
    TCGAGACAAG TCAACGCTGA TGAGTTGCAA CCTGGTGTCT GCTATCTGCA GATAACTGCT
    GTTGATCCTG TAATGGAGGA TGAGGACCTA GGGAGTAGAA GGGAAAGGAT TTTCTCTTTA
    TCTACTGGTA CTGTCCGGGC CCGTGTGTTC GACCGTTTTC TTTTTGATAC ACCATTCACA
    AAGAATGGAA AAACACAAGG TGGCCTTGAA GATCAGTGGA AGAGGCGCAC AGTACTCCAG
    ACAGAGGGTT CCTTTCCTGC TTTGGTGAAT CGTCTGTTAG TGATCAAATC AGAATCTCTA
    GAGTTTTCTC CTGTTGAGAA TGCGATTGGA ATGATCGAGA CAAGGACAGC TGCCTTGCGA
    AATGAACTAG AAGAACCACG CAGTTCGGAA GGTGACCAAC TCCCAAGACT CCAAAGCCTG
    CAGAGGATAC TCCAAGGAAG TGTGGCTGTT CAGGTAAATA GTGGTGTATT AAGTGTGTGC
    ACTGCATTCT TATCTGGTGA GCCCGCAACT AGGCTCCGAT CTCAAGAACT GCAGCAGCTC
    ATCGCTGCAT TGCTGGAATT CATGGCCGTC TGCAAGCGCG CAATCCGTGT GCATTTTAGA
    TTGATAGGGG AAGAAGACCA GGAATTCCAC ACTCAACTAG TCAATGGCTT TCAGTCGCTC
    ACAGCTGAGC TTTCACACTA TATTCCTGCA ATTCTTTCAG AGCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOa30426
    Clone ID Related Accession (Same CDS sequence) XM_015775852.1 , XM_015775852.2 , XM_015775853.1
    Accession Version XM_015775853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-02-29
    Organism Oryza sativa Japonica Group(Japanese rice)
    Product guanine nucleotide exchange factor SPIKE 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATTCCG CGGCGGCGGC GGGGGAGGGG CAGCGGTTCA AGCGCATTCC GCGGCAGTCG 
    TTGGCGGGGA ACCTGGAGCT GGATCCACTG CTTAATGAAA ATTTAGATCA ATGGCCACAT
    CTAAATGAGC TGGTTCAGTG CTATAAGGCT GATTTTGTGA AAGACGACTG CAAATATGGA
    CGATATGAAA GTGTTGCACC ACCATCTTTT CAGAATCAAA TTTTTGAGGG ACCTGATACT
    GACTTAGAAA CAGAATTGCA GCTTAGCAAT GATAGGCAAT CCAAGCCTGA TGAAGTTACT
    GAAGATGATA TGCCAAGCAC ATCAGGGAGA CAATTATATG AGACTGAAGT GCCCGCTTCA
    TCCTCAAAAA AACACTGCAG TCTATCACCT TTACCAGCAT ATGAACCGGC ATTTGATTGG
    GAAAATGAGA GATCTTTGAT ATTCGGCCAA CGGGTGCCAG AAAGTGTCCC TGCAATAAAC
    AGCAGTGGAT TGAAGATAAC TGTCAAGGTG TTATCTTTGT CATTTCAAGC TGGATTAGTT
    GAGCCTTTTA GTGGTACAAT CTGCTTGTAC AACAGAGACA GAAGAGAAAA ATTGTCAGAA
    GATTTCTATT TTCATATACT CCCAACAGAA ATGCAAGATG CTCAGATTTC TCTAGACCGC
    CGAGGTGTTT TTTCATTGGA TGCCCCTTCA CCATCAGTGT GCCTTCTGAT TCAGCTAGAG
    AAGGCTGCCA CTGAGGAAGG GGGAGTAACA CCCTCTGTTT ATTCTCGTAA AGAACCTGTT
    CACTTGACTG ATAAAGAGAA GCAAAAATTG CAAGTCTGGT CTCGAATCAT GCCATATAGA
    GAGTCGTTCG CATGGGCTAT GATTCCACTG TTTGAGAACA ACCAGGCTGG TGGTGCTGCC
    TCTCCCAGTA GCCCTCTAGC ACCAAGTATG TCAGGCTCAA GTTCTCAAGA TAGTATAGTG
    GAGCCTATTT CCAAGCTCAC TTTGGATGGA AAGCTCAATC ATTATTCAAG TGGAAGCTCA
    GTTATTGTAG AGATATCAAA TTTGAACAAA GTGAAGGAAA GCTACATAGA GGACTCCCTC
    CAAGACCCCA AAAGAAAAGT ACACAAACCA GTGAAAGGTG TACTGAGGTT AGAGGTGGAA
    AAACTTCATA ATGGCCACAA CGACATGGAT AACATTTCTG AGGGCGGTAG CATGGCCAAT
    GATTTGAATG ATGCTGGTGA ACTCAATAAT GGTAGATACA ACCGGAGTAG CTTTGATGGG
    ATCCATGGTT CTTTGAATTC TAGTGCCGTG GCCCAGAAAG ATGCACATCA GAATGGTCAA
    GCTTCCAATA CAGAGAGTGG TGAAAATTTT CAAGCTTTTG ACTTTCGGAT GATGACCCGA
    AGTGAGCCAT TTTCACAACT CTTCCATTGT CTCTATGTGT ACCCACTGAC TATTAGCTTG
    GGCCGTAAAA GAAATCTATT TGTAAGAGTG GAATTGAGAA AGGATGATTC TGACATCCGC
    AAGCCTCCAT TAGAGGCTGT TCATCCAAGG GATCGGAACA CAACACTACA GAAGTGGGCG
    CATACTCAGA TTGCTGTTGG AACGAGAATG GCTTGCTACC ATGATGAAGT TAAAATCAGT
    CTGCCTGCTC TCTTGACACC CCAACATCAT CTTCTGTTCA CATTTTACCA TGTAGATCTC
    CAAATGAAAC CTGAAGCCCC CAAACCAGTT GTCGTGGGAT ATGCTGTTCT TCCACTGTCG
    ACACACATTC AGTTACTTTC AGACGTATCT TTGCCAATTT TAAGAGAACT TGTTCCACAT
    TACCTGCAGG AAAGTGGAAA GGAAAGAATG GACTATCTGG AGGATGGCAA AACTGTTTTT
    AGGTTGCGAT TAAGACTTTG TTCTTCATTA TTCCCAGTTA ATGAAAGAAT AAGAGACTTT
    TTTGTCGAGT ATGACCGCCA TACACTTCAT ACAAGTCCAC CTTGGGGTTC TGAGCTTCTT
    GAGGCCATAA ACAGTTTAAA GAATGTTGAA TCTACTGCAC TGTTGCAATT TCTCCAACCC
    ATACTTAATA TGCTACTTCA TCTTATCGGT GATGGTGGTG AAACCCTTCA GGTTGCCGCA
    TTTCGAGCTA TGGTCAATAT CCTAACCCGG GTCCAGCAGG AATCATCTGA TGGGGCTGAG
    AGAAATCGGT TTCTTGTTAG TTATGTTGAT TATGCTTTTG ATGACTTTGG TGATCGGCAA
    GCACCTGTAT ACCCTGGTCT GTCTACTGTT TGGGGAAGCC TGGCTCGGAG TAAGGCTAAA
    GGTTATAGAG TTGGGCCAGT CTATGATGAT GTGCTGGCAA TGGCTTGGTT TTTTCTGGAG
    CTTATTGTAA AATCGATGGG ATTGGAACAA AGTCGTCTAT TTTACCATAA TCTTCCACTA
    GGTGAAGATG TCCCACCGCT ACAGTTGAAA GATGGTGTCT TCCGATGTAT CATGCAGCTG
    TTTGATTGCC TTCTAACTGA GGTTCATGAG CGCTGTAAAA AGGGTTTAAG CTTGGCGAAG
    CGCTTGAACA GCACACTTGC TTTCTTTTGT TATGATCTTC TATCAATTAT TGAGCCACGC
    CAAGTTTTTG AGCTGGTTTC ATTGTATATG GACAAGTTTG CAGGAGTTTG CCAGTCAATT
    CTCCATGATT GCAAATTGAC ATTCCTTCAG ATAATATGTG ACCATGATCT TTTTGTTGAG
    ATGCCTGGTC GTGACCCCTC TGATAGAAAT TATCTCTCGT CAGTACTTAT TCAAGAGATT
    TTTCTCACCC TTGACCATGA TGATCTATCT CAGCGAGCGA AAGCAGCCCG TATTTTGGTT
    GTTCTAATAT GCAAGCATGA ATTTGATGCA CGATATCAAA AAAGTGAAGA CAAGCTGTAT
    ATTGCTCAGC TGTATTTTTC TCTTATAGGA CAGATTCTAG ATGAGATGCC TGTATTCTAT
    AATCTAAATG CTGTTGAAAA GCGTGAGGTG CTGGTAGTCA TTTTGCAAAT CATAAGGAAC
    TTGGACGACA TGACTCTGAT CAAAGCATGG CAACAGAGCA TTGCTAGAAC CAGATTGTTC
    TTCAAGCTAC TTGAAGAATG CATTACCCAT TTTGAGCACA ACAAAACAGG AGACAGCTTG
    TTACTAGGCT CAAGTTCACG GAGCCCTGAT GCTGAGCGCC CTGCATCTCC AAAGTACTCC
    GATCGATTAT CCCCATCAGT TAATGCATAT TTGTCTGAGG CATCACGCCA TGAAATAAGG
    CCTCAGGGAA CACCAGAAAA TGGATACATG TGGAACAGGG TTAGTCCTCA ACTGAGCTCT
    CCTAACCAGC CCTATTCATT GAGAGAAGCA CTTGCTCAAG CGCAGTCTTC AAGGATTGGA
    TCAACCGCGA GGGCACTGAG AGAATCTCTA CATCCAGTGC TGAGACAAAA GTTGGAACTG
    TGGGAAGAAA ATCTCAGTAC TGCTGTGAGC CTGGAAGTGT TGGGAATAAT TGATAAGTTT
    TCAGTCGCTG CAGCTTCTCG TAGCATCACT ACTGATTACG CAAAGCTAGA TTGTGTAACA
    TCTGTATTAA TGGGTCTTTT ATCAAGGAGC CAGCCCTTAG CTTTTTGGAA AGCTTTCCTT
    CCTGTGGTGT ATAATATATT TAACCTCCAT GGCGCAACAC TGATGGCAAG GGAGAATGAT
    CGCTTCTTGA AGCAAATTGC TTTTCATCTT TTGCGCCTTG CTGTTTTCCG GAATGATTCT
    ATTAGAAAAA GGGCTGTTGT TGGGTTGCAG ATTCTTGTTA GGAACTCGTT TAATTATTTT
    AAGAACACCA CAAGGTTGAG GGTGATGTTG ACAATCACTT TGTCAGAACT TATGTCTGAT
    GTGCAAGTAA CTCAGATGAA ATCTGATGGC TCTCTTGAGG AAAGTGGTGA AACTCGGTGC
    CTTAGGAAAT CACTAGAGGA AATGGCTGAT GTACGAAGTA AGGATCTCTT GAAAGATTGT
    GGCCTCCCTG TTAATGCACT GGAGGCAGCG CCTGAAGGGT CGACTGATAA TAGGTGGTCT
    TGGGTAGAAG TTAAACATCT ATCCAAATGT CTAGTCCAGG CCCTTGATGC TGGTCTTGAA
    CATGCCCTCT TGGGCTCTGA AATGACTCTG GACAGGTACG CTGCTGCTGA GGGTTTCTAT
    AAGCTAGCAA TGGCCTACGC GCCTGTGCCA GATCTTCATA TAATGTGGTT ACTGCATCTA
    TGTGATGCAC ATCAGGAGAT GCAATCATGG GCTGAAGCTG CACAATGTGC AGTTGCTGTA
    GCTGGTGTTA TCATGCAGGC ACTTGTTGGA AGGAATGATG CGGTGTGGAG TAAGGAGCAT
    GTTGCTTCCC TATGTAAGAT TTGCCCTATT GTGAACACTG ATGTTAGCTC AGAAGCATCT
    GCAGCAGAAG TTGAGGGATA TGGTGCATCC AAGCTTACTG TGGATTCAGC TGTCAAGTAT
    CTTCAGCTGG CAAACAAACT TTTTGCACAA GCCGAACTCT ATCACTTTTG TGCTAGTATT
    CAGGAACTCA TCATTCCTGT GTATAAAAGC AGAAGGGCAT ATGGGCATCT GGCTAAGTGT
    CATACATCAC TCAAGGATAT CTATGAGTCA ATTCTTGAAC AGGAGGCTAG TCCTATACCA
    TTCATTGATG CTACCTACTA CCGGGTAGGG TTTTATGGGG AGCGGTTTGG CAAACTCAAC
    AAAAAGGAGT ATGTGTTCAG AGAGCCAAGA GATGTTCGTC TTGGTGACAT AATGGAGAAG
    CTTAGTCATA TCTATGAGGC TAAAATGGAT GGCAATCACA CCTTACACAT CATTCCGGAT
    TCGAGACAAG TCAACGCTGA TGAGTTGCAA CCTGGTGTCT GCTATCTGCA GATAACTGCT
    GTTGATCCTG TAATGGAGGA TGAGGACCTA GGGAGTAGAA GGGAAAGGAT TTTCTCTTTA
    TCTACTGGTA CTGTCCGGGC CCGTGTGTTC GACCGTTTTC TTTTTGATAC ACCATTCACA
    AAGAATGGAA AAACACAAGG TGGCCTTGAA GATCAGTGGA AGAGGCGCAC AGTACTCCAG
    ACAGAGGGTT CCTTTCCTGC TTTGGTGAAT CGTCTGTTAG TGATCAAATC AGAATCTCTA
    GAGTTTTCTC CTGTTGAGAA TGCGATTGGA ATGATCGAGA CAAGGACAGC TGCCTTGCGA
    AATGAACTAG AAGAACCACG CAGTTCGGAA GGTGACCAAC TCCCAAGACT CCAAAGCCTG
    CAGAGGATAC TCCAAGGAAG TGTGGCTGTT CAGGTAAATA GTGGTGTATT AAGTGTGTGC
    ACTGCATTCT TATCTGGTGA GCCCGCAACT AGGCTCCGAT CTCAAGAACT GCAGCAGCTC
    ATCGCTGCAT TGCTGGAATT CATGGCCGTC TGCAAGCGCG CAATCCGTGT GCATTTTAGA
    TTGATAGGGG AAGAAGACCA GGAATTCCAC ACTCAACTAG TCAATGGCTT TCAGTCGCTC
    ACAGCTGAGC TTTCACACTA TATTCCTGCA ATTCTTTCAG AGCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015631339.1
    CDS313..5820
    Translation

    Target ORF information:

    RefSeq Version XM_015775853.1
    Organism Oryza sativa Japonica Group(Japanese rice)
    Definition Oryza sativa Japonica Group guanine nucleotide exchange factor SPIKE 1 (LOC4332718), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015775853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGATTCCG CGGCGGCGGC GGGGGAGGGG CAGCGGTTCA AGCGCATTCC GCGGCAGTCG 
    TTGGCGGGGA ACCTGGAGCT GGATCCACTG CTTAATGAAA ATTTAGATCA ATGGCCACAT
    CTAAATGAGC TGGTTCAGTG CTATAAGGCT GATTTTGTGA AAGACGACTG CAAATATGGA
    CGATATGAAA GTGTTGCACC ACCATCTTTT CAGAATCAAA TTTTTGAGGG ACCTGATACT
    GACTTAGAAA CAGAATTGCA GCTTAGCAAT GATAGGCAAT CCAAGCCTGA TGAAGTTACT
    GAAGATGATA TGCCAAGCAC ATCAGGGAGA CAATTATATG AGACTGAAGT GCCCGCTTCA
    TCCTCAAAAA AACACTGCAG TCTATCACCT TTACCAGCAT ATGAACCGGC ATTTGATTGG
    GAAAATGAGA GATCTTTGAT ATTCGGCCAA CGGGTGCCAG AAAGTGTCCC TGCAATAAAC
    AGCAGTGGAT TGAAGATAAC TGTCAAGGTG TTATCTTTGT CATTTCAAGC TGGATTAGTT
    GAGCCTTTTA GTGGTACAAT CTGCTTGTAC AACAGAGACA GAAGAGAAAA ATTGTCAGAA
    GATTTCTATT TTCATATACT CCCAACAGAA ATGCAAGATG CTCAGATTTC TCTAGACCGC
    CGAGGTGTTT TTTCATTGGA TGCCCCTTCA CCATCAGTGT GCCTTCTGAT TCAGCTAGAG
    AAGGCTGCCA CTGAGGAAGG GGGAGTAACA CCCTCTGTTT ATTCTCGTAA AGAACCTGTT
    CACTTGACTG ATAAAGAGAA GCAAAAATTG CAAGTCTGGT CTCGAATCAT GCCATATAGA
    GAGTCGTTCG CATGGGCTAT GATTCCACTG TTTGAGAACA ACCAGGCTGG TGGTGCTGCC
    TCTCCCAGTA GCCCTCTAGC ACCAAGTATG TCAGGCTCAA GTTCTCAAGA TAGTATAGTG
    GAGCCTATTT CCAAGCTCAC TTTGGATGGA AAGCTCAATC ATTATTCAAG TGGAAGCTCA
    GTTATTGTAG AGATATCAAA TTTGAACAAA GTGAAGGAAA GCTACATAGA GGACTCCCTC
    CAAGACCCCA AAAGAAAAGT ACACAAACCA GTGAAAGGTG TACTGAGGTT AGAGGTGGAA
    AAACTTCATA ATGGCCACAA CGACATGGAT AACATTTCTG AGGGCGGTAG CATGGCCAAT
    GATTTGAATG ATGCTGGTGA ACTCAATAAT GGTAGATACA ACCGGAGTAG CTTTGATGGG
    ATCCATGGTT CTTTGAATTC TAGTGCCGTG GCCCAGAAAG ATGCACATCA GAATGGTCAA
    GCTTCCAATA CAGAGAGTGG TGAAAATTTT CAAGCTTTTG ACTTTCGGAT GATGACCCGA
    AGTGAGCCAT TTTCACAACT CTTCCATTGT CTCTATGTGT ACCCACTGAC TATTAGCTTG
    GGCCGTAAAA GAAATCTATT TGTAAGAGTG GAATTGAGAA AGGATGATTC TGACATCCGC
    AAGCCTCCAT TAGAGGCTGT TCATCCAAGG GATCGGAACA CAACACTACA GAAGTGGGCG
    CATACTCAGA TTGCTGTTGG AACGAGAATG GCTTGCTACC ATGATGAAGT TAAAATCAGT
    CTGCCTGCTC TCTTGACACC CCAACATCAT CTTCTGTTCA CATTTTACCA TGTAGATCTC
    CAAATGAAAC CTGAAGCCCC CAAACCAGTT GTCGTGGGAT ATGCTGTTCT TCCACTGTCG
    ACACACATTC AGTTACTTTC AGACGTATCT TTGCCAATTT TAAGAGAACT TGTTCCACAT
    TACCTGCAGG AAAGTGGAAA GGAAAGAATG GACTATCTGG AGGATGGCAA AACTGTTTTT
    AGGTTGCGAT TAAGACTTTG TTCTTCATTA TTCCCAGTTA ATGAAAGAAT AAGAGACTTT
    TTTGTCGAGT ATGACCGCCA TACACTTCAT ACAAGTCCAC CTTGGGGTTC TGAGCTTCTT
    GAGGCCATAA ACAGTTTAAA GAATGTTGAA TCTACTGCAC TGTTGCAATT TCTCCAACCC
    ATACTTAATA TGCTACTTCA TCTTATCGGT GATGGTGGTG AAACCCTTCA GGTTGCCGCA
    TTTCGAGCTA TGGTCAATAT CCTAACCCGG GTCCAGCAGG AATCATCTGA TGGGGCTGAG
    AGAAATCGGT TTCTTGTTAG TTATGTTGAT TATGCTTTTG ATGACTTTGG TGATCGGCAA
    GCACCTGTAT ACCCTGGTCT GTCTACTGTT TGGGGAAGCC TGGCTCGGAG TAAGGCTAAA
    GGTTATAGAG TTGGGCCAGT CTATGATGAT GTGCTGGCAA TGGCTTGGTT TTTTCTGGAG
    CTTATTGTAA AATCGATGGG ATTGGAACAA AGTCGTCTAT TTTACCATAA TCTTCCACTA
    GGTGAAGATG TCCCACCGCT ACAGTTGAAA GATGGTGTCT TCCGATGTAT CATGCAGCTG
    TTTGATTGCC TTCTAACTGA GGTTCATGAG CGCTGTAAAA AGGGTTTAAG CTTGGCGAAG
    CGCTTGAACA GCACACTTGC TTTCTTTTGT TATGATCTTC TATCAATTAT TGAGCCACGC
    CAAGTTTTTG AGCTGGTTTC ATTGTATATG GACAAGTTTG CAGGAGTTTG CCAGTCAATT
    CTCCATGATT GCAAATTGAC ATTCCTTCAG ATAATATGTG ACCATGATCT TTTTGTTGAG
    ATGCCTGGTC GTGACCCCTC TGATAGAAAT TATCTCTCGT CAGTACTTAT TCAAGAGATT
    TTTCTCACCC TTGACCATGA TGATCTATCT CAGCGAGCGA AAGCAGCCCG TATTTTGGTT
    GTTCTAATAT GCAAGCATGA ATTTGATGCA CGATATCAAA AAAGTGAAGA CAAGCTGTAT
    ATTGCTCAGC TGTATTTTTC TCTTATAGGA CAGATTCTAG ATGAGATGCC TGTATTCTAT
    AATCTAAATG CTGTTGAAAA GCGTGAGGTG CTGGTAGTCA TTTTGCAAAT CATAAGGAAC
    TTGGACGACA TGACTCTGAT CAAAGCATGG CAACAGAGCA TTGCTAGAAC CAGATTGTTC
    TTCAAGCTAC TTGAAGAATG CATTACCCAT TTTGAGCACA ACAAAACAGG AGACAGCTTG
    TTACTAGGCT CAAGTTCACG GAGCCCTGAT GCTGAGCGCC CTGCATCTCC AAAGTACTCC
    GATCGATTAT CCCCATCAGT TAATGCATAT TTGTCTGAGG CATCACGCCA TGAAATAAGG
    CCTCAGGGAA CACCAGAAAA TGGATACATG TGGAACAGGG TTAGTCCTCA ACTGAGCTCT
    CCTAACCAGC CCTATTCATT GAGAGAAGCA CTTGCTCAAG CGCAGTCTTC AAGGATTGGA
    TCAACCGCGA GGGCACTGAG AGAATCTCTA CATCCAGTGC TGAGACAAAA GTTGGAACTG
    TGGGAAGAAA ATCTCAGTAC TGCTGTGAGC CTGGAAGTGT TGGGAATAAT TGATAAGTTT
    TCAGTCGCTG CAGCTTCTCG TAGCATCACT ACTGATTACG CAAAGCTAGA TTGTGTAACA
    TCTGTATTAA TGGGTCTTTT ATCAAGGAGC CAGCCCTTAG CTTTTTGGAA AGCTTTCCTT
    CCTGTGGTGT ATAATATATT TAACCTCCAT GGCGCAACAC TGATGGCAAG GGAGAATGAT
    CGCTTCTTGA AGCAAATTGC TTTTCATCTT TTGCGCCTTG CTGTTTTCCG GAATGATTCT
    ATTAGAAAAA GGGCTGTTGT TGGGTTGCAG ATTCTTGTTA GGAACTCGTT TAATTATTTT
    AAGAACACCA CAAGGTTGAG GGTGATGTTG ACAATCACTT TGTCAGAACT TATGTCTGAT
    GTGCAAGTAA CTCAGATGAA ATCTGATGGC TCTCTTGAGG AAAGTGGTGA AACTCGGTGC
    CTTAGGAAAT CACTAGAGGA AATGGCTGAT GTACGAAGTA AGGATCTCTT GAAAGATTGT
    GGCCTCCCTG TTAATGCACT GGAGGCAGCG CCTGAAGGGT CGACTGATAA TAGGTGGTCT
    TGGGTAGAAG TTAAACATCT ATCCAAATGT CTAGTCCAGG CCCTTGATGC TGGTCTTGAA
    CATGCCCTCT TGGGCTCTGA AATGACTCTG GACAGGTACG CTGCTGCTGA GGGTTTCTAT
    AAGCTAGCAA TGGCCTACGC GCCTGTGCCA GATCTTCATA TAATGTGGTT ACTGCATCTA
    TGTGATGCAC ATCAGGAGAT GCAATCATGG GCTGAAGCTG CACAATGTGC AGTTGCTGTA
    GCTGGTGTTA TCATGCAGGC ACTTGTTGGA AGGAATGATG CGGTGTGGAG TAAGGAGCAT
    GTTGCTTCCC TATGTAAGAT TTGCCCTATT GTGAACACTG ATGTTAGCTC AGAAGCATCT
    GCAGCAGAAG TTGAGGGATA TGGTGCATCC AAGCTTACTG TGGATTCAGC TGTCAAGTAT
    CTTCAGCTGG CAAACAAACT TTTTGCACAA GCCGAACTCT ATCACTTTTG TGCTAGTATT
    CAGGAACTCA TCATTCCTGT GTATAAAAGC AGAAGGGCAT ATGGGCATCT GGCTAAGTGT
    CATACATCAC TCAAGGATAT CTATGAGTCA ATTCTTGAAC AGGAGGCTAG TCCTATACCA
    TTCATTGATG CTACCTACTA CCGGGTAGGG TTTTATGGGG AGCGGTTTGG CAAACTCAAC
    AAAAAGGAGT ATGTGTTCAG AGAGCCAAGA GATGTTCGTC TTGGTGACAT AATGGAGAAG
    CTTAGTCATA TCTATGAGGC TAAAATGGAT GGCAATCACA CCTTACACAT CATTCCGGAT
    TCGAGACAAG TCAACGCTGA TGAGTTGCAA CCTGGTGTCT GCTATCTGCA GATAACTGCT
    GTTGATCCTG TAATGGAGGA TGAGGACCTA GGGAGTAGAA GGGAAAGGAT TTTCTCTTTA
    TCTACTGGTA CTGTCCGGGC CCGTGTGTTC GACCGTTTTC TTTTTGATAC ACCATTCACA
    AAGAATGGAA AAACACAAGG TGGCCTTGAA GATCAGTGGA AGAGGCGCAC AGTACTCCAG
    ACAGAGGGTT CCTTTCCTGC TTTGGTGAAT CGTCTGTTAG TGATCAAATC AGAATCTCTA
    GAGTTTTCTC CTGTTGAGAA TGCGATTGGA ATGATCGAGA CAAGGACAGC TGCCTTGCGA
    AATGAACTAG AAGAACCACG CAGTTCGGAA GGTGACCAAC TCCCAAGACT CCAAAGCCTG
    CAGAGGATAC TCCAAGGAAG TGTGGCTGTT CAGGTAAATA GTGGTGTATT AAGTGTGTGC
    ACTGCATTCT TATCTGGTGA GCCCGCAACT AGGCTCCGAT CTCAAGAACT GCAGCAGCTC
    ATCGCTGCAT TGCTGGAATT CATGGCCGTC TGCAAGCGCG CAATCCGTGT GCATTTTAGA
    TTGATAGGGG AAGAAGACCA GGAATTCCAC ACTCAACTAG TCAATGGCTT TCAGTCGCTC
    ACAGCTGAGC TTTCACACTA TATTCCTGCA ATTCTTTCAG AGCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.