LOC4332529 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC4332529
Entrez Gene ID	4332529
Full Name	ubiquitin-activating enzyme E1 3
Gene Type	protein-coding
Organism	Oryza sativa Japonica Group(Japanese rice)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015777317.2	XP_015632803.1	ubiquitin-activating enzyme E1 3
XM_015777317.1	XP_015632803.1	ubiquitin-activating enzyme E1 3
XM_015777318.1	XP_015632804.1	ubiquitin-activating enzyme E1 3
XM_015777318.2	XP_015632804.1	ubiquitin-activating enzyme E1 3
XM_015777316.1	XP_015632802.1	ubiquitin-activating enzyme E1 3
XM_015777316.2	XP_015632802.1	ubiquitin-activating enzyme E1 3

Pathways from BioSystems

Homologies

	UBA1	XP_001092372.2
Mus musculus (house mouse)	Uba1	NP_033483.2
Drosophila melanogaster (fruit fly)	Uba1	NP_477310.2
Saccharomyces cerevisiae (baker's yeast)	UBA1	NP_012712.1
Homo sapiens (human)	UBA1	NP_003325.2
Canis lupus familiaris (dog)	UBA1	XP_005641334.1
Bos taurus (cattle)	UBA1	NP_001095947.1
Danio rerio (zebrafish)	uba1	NP_998227.1
Caenorhabditis elegans (roundworm)	uba-1	NP_001033404.1
Oryza sativa (rice)	Os03g0294900	NP_001049821.1
Schizosaccharomyces pombe (fission yeast)	ptr3	XP_001713148.1
Magnaporthe oryzae (rice blast fungus)	MGG_01409	XP_003714354.1
Oryza sativa (rice)	Os07g0692900	NP_001060725.1
Rattus norvegicus (Norway rat)	Uba1	NP_001014102.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011872	XP_552371.3
Kluyveromyces lactis	KLLA0B14278g	XP_452166.1
Eremothecium gossypii	AGOS_AFR433C	NP_985980.1
Neurospora crassa	NCU04370	XP_956143.1

Gene Ontology

The following LOC4332529 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC4332529 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOa07119	XM_015777317.2 Latest version!	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OOa07119	XM_015777316.2 Latest version!	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OOa07119	XM_015777318.2 Latest version!	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OOa07119	XM_015777317.1	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OOa07119	XM_015777318.1	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OOa07119	XM_015777316.1	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC4332529 ( XM_015777317.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632803.1 Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOa07119
Clone ID Related Accession (Same CDS sequence)	XM_015777317.2 , XM_015777317.1 , XM_015777318.1 , XM_015777318.2 , XM_015777316.1 , XM_015777316.2
Accession Version	XM_015777317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	ubiquitin-activating enzyme E1 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015777317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632803.1
CDS	290..3484
Translation	MLPTKRANGAEAESSSDAPAKKARVGASASEAEAMVAGEAGGGGGGVSGNGSEVAEIDEDLHSRQLAVYGRETMRRLFASNVLVSGLNGLGAEIAKNLALAGVKSITLHDMGNVEMWDLSGNFFLSEDDIGKNRAVACTAKLQELNNAVLISTLTEDLTNEHLSKFQAVVFTDISLDKAFEFDDYCRNHQPSISFIKAEVCGLFGSVFCDFGPKFTVLDVDGEEPHTGIIASISNDNPAMISCVDDERLEFQDGDLVVFSEVQGMTELNDGKPRKIINARPYSFCIQEDTSKFGIYAKGGIVTQVKEPINLEFKSLRDSIREPGNFLLSDFSKFDRPPLLHFAFLALDKFRKEFGRFPGAGCDQDAQRFIEFVASVNEATIDYKMDELDGKLLRNFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPQYQFFYFDSAESLPTYPLDSKDLKPLNSRYDAQISVFGSKLQKKMRDANVFVVGSGALGCEFLKNLALMGVSCGLKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAAAAASAINSSLHINALQNRACPETEHVFHDKFWEGLDVIINALDNVNARMYMDMRCLYFQKPLLESGTLGPKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFISNPAQYAAAMRKAGDAQARELLERVCECLDKERCDGFEDCIAWARLKFEDYFANRVKQLTFTFPEDAVTSTGAFFWSAPKRFPRPLQFSTVNSSHIHFILAASILRAVSFGISIPDWAKNTSNLVDAVSKVVVPEFEPKSGVKIETDEKASNISSASVDDASVIEDLLTKLEASAKKLPPGFQMKAIQFEKDDDTNFHMDLIAGLANMRARNYGIQEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLAGGHPVEDYRNSFANLAIPMFSMAEPLPPKVIKHQDMRWTIWDRWSIEGNITVAELLKWLSDKGLSAYSVSCGTSLLYNTMFPRHKDRVNKKLVDVAKEVAKVDVPAYRRHLDVVVACEDDDGNDVDIPLISIYFR

Target ORF information:

RefSeq Version	XM_015777317.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777317.2

ORF Insert Sequence:

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332529 ( XM_015777317.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632803.1 Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOa07119
Clone ID Related Accession (Same CDS sequence)	XM_015777317.2 , XM_015777317.1 , XM_015777318.1 , XM_015777318.2 , XM_015777316.1 , XM_015777316.2
Accession Version	XM_015777317.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	ubiquitin-activating enzyme E1 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632803.1
CDS	272..3466
Translation	MLPTKRANGAEAESSSDAPAKKARVGASASEAEAMVAGEAGGGGGGVSGNGSEVAEIDEDLHSRQLAVYGRETMRRLFASNVLVSGLNGLGAEIAKNLALAGVKSITLHDMGNVEMWDLSGNFFLSEDDIGKNRAVACTAKLQELNNAVLISTLTEDLTNEHLSKFQAVVFTDISLDKAFEFDDYCRNHQPSISFIKAEVCGLFGSVFCDFGPKFTVLDVDGEEPHTGIIASISNDNPAMISCVDDERLEFQDGDLVVFSEVQGMTELNDGKPRKIINARPYSFCIQEDTSKFGIYAKGGIVTQVKEPINLEFKSLRDSIREPGNFLLSDFSKFDRPPLLHFAFLALDKFRKEFGRFPGAGCDQDAQRFIEFVASVNEATIDYKMDELDGKLLRNFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPQYQFFYFDSAESLPTYPLDSKDLKPLNSRYDAQISVFGSKLQKKMRDANVFVVGSGALGCEFLKNLALMGVSCGLKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAAAAASAINSSLHINALQNRACPETEHVFHDKFWEGLDVIINALDNVNARMYMDMRCLYFQKPLLESGTLGPKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFISNPAQYAAAMRKAGDAQARELLERVCECLDKERCDGFEDCIAWARLKFEDYFANRVKQLTFTFPEDAVTSTGAFFWSAPKRFPRPLQFSTVNSSHIHFILAASILRAVSFGISIPDWAKNTSNLVDAVSKVVVPEFEPKSGVKIETDEKASNISSASVDDASVIEDLLTKLEASAKKLPPGFQMKAIQFEKDDDTNFHMDLIAGLANMRARNYGIQEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLAGGHPVEDYRNSFANLAIPMFSMAEPLPPKVIKHQDMRWTIWDRWSIEGNITVAELLKWLSDKGLSAYSVSCGTSLLYNTMFPRHKDRVNKKLVDVAKEVAKVDVPAYRRHLDVVVACEDDDGNDVDIPLISIYFR

Target ORF information:

RefSeq Version	XM_015777317.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777317.1

ORF Insert Sequence:

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332529 ( XM_015777318.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632804.1 Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOa07119
Clone ID Related Accession (Same CDS sequence)	XM_015777317.2 , XM_015777317.1 , XM_015777318.1 , XM_015777318.2 , XM_015777316.1 , XM_015777316.2
Accession Version	XM_015777318.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	ubiquitin-activating enzyme E1 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632804.1
CDS	170..3364
Translation	MLPTKRANGAEAESSSDAPAKKARVGASASEAEAMVAGEAGGGGGGVSGNGSEVAEIDEDLHSRQLAVYGRETMRRLFASNVLVSGLNGLGAEIAKNLALAGVKSITLHDMGNVEMWDLSGNFFLSEDDIGKNRAVACTAKLQELNNAVLISTLTEDLTNEHLSKFQAVVFTDISLDKAFEFDDYCRNHQPSISFIKAEVCGLFGSVFCDFGPKFTVLDVDGEEPHTGIIASISNDNPAMISCVDDERLEFQDGDLVVFSEVQGMTELNDGKPRKIINARPYSFCIQEDTSKFGIYAKGGIVTQVKEPINLEFKSLRDSIREPGNFLLSDFSKFDRPPLLHFAFLALDKFRKEFGRFPGAGCDQDAQRFIEFVASVNEATIDYKMDELDGKLLRNFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPQYQFFYFDSAESLPTYPLDSKDLKPLNSRYDAQISVFGSKLQKKMRDANVFVVGSGALGCEFLKNLALMGVSCGLKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAAAAASAINSSLHINALQNRACPETEHVFHDKFWEGLDVIINALDNVNARMYMDMRCLYFQKPLLESGTLGPKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFISNPAQYAAAMRKAGDAQARELLERVCECLDKERCDGFEDCIAWARLKFEDYFANRVKQLTFTFPEDAVTSTGAFFWSAPKRFPRPLQFSTVNSSHIHFILAASILRAVSFGISIPDWAKNTSNLVDAVSKVVVPEFEPKSGVKIETDEKASNISSASVDDASVIEDLLTKLEASAKKLPPGFQMKAIQFEKDDDTNFHMDLIAGLANMRARNYGIQEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLAGGHPVEDYRNSFANLAIPMFSMAEPLPPKVIKHQDMRWTIWDRWSIEGNITVAELLKWLSDKGLSAYSVSCGTSLLYNTMFPRHKDRVNKKLVDVAKEVAKVDVPAYRRHLDVVVACEDDDGNDVDIPLISIYFR

Target ORF information:

RefSeq Version	XM_015777318.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777318.1

ORF Insert Sequence:

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332529 ( XM_015777318.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632804.1 Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOa07119
Clone ID Related Accession (Same CDS sequence)	XM_015777317.2 , XM_015777317.1 , XM_015777318.1 , XM_015777318.2 , XM_015777316.1 , XM_015777316.2
Accession Version	XM_015777318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	ubiquitin-activating enzyme E1 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015777318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632804.1
CDS	183..3377
Translation	MLPTKRANGAEAESSSDAPAKKARVGASASEAEAMVAGEAGGGGGGVSGNGSEVAEIDEDLHSRQLAVYGRETMRRLFASNVLVSGLNGLGAEIAKNLALAGVKSITLHDMGNVEMWDLSGNFFLSEDDIGKNRAVACTAKLQELNNAVLISTLTEDLTNEHLSKFQAVVFTDISLDKAFEFDDYCRNHQPSISFIKAEVCGLFGSVFCDFGPKFTVLDVDGEEPHTGIIASISNDNPAMISCVDDERLEFQDGDLVVFSEVQGMTELNDGKPRKIINARPYSFCIQEDTSKFGIYAKGGIVTQVKEPINLEFKSLRDSIREPGNFLLSDFSKFDRPPLLHFAFLALDKFRKEFGRFPGAGCDQDAQRFIEFVASVNEATIDYKMDELDGKLLRNFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPQYQFFYFDSAESLPTYPLDSKDLKPLNSRYDAQISVFGSKLQKKMRDANVFVVGSGALGCEFLKNLALMGVSCGLKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAAAAASAINSSLHINALQNRACPETEHVFHDKFWEGLDVIINALDNVNARMYMDMRCLYFQKPLLESGTLGPKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFISNPAQYAAAMRKAGDAQARELLERVCECLDKERCDGFEDCIAWARLKFEDYFANRVKQLTFTFPEDAVTSTGAFFWSAPKRFPRPLQFSTVNSSHIHFILAASILRAVSFGISIPDWAKNTSNLVDAVSKVVVPEFEPKSGVKIETDEKASNISSASVDDASVIEDLLTKLEASAKKLPPGFQMKAIQFEKDDDTNFHMDLIAGLANMRARNYGIQEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLAGGHPVEDYRNSFANLAIPMFSMAEPLPPKVIKHQDMRWTIWDRWSIEGNITVAELLKWLSDKGLSAYSVSCGTSLLYNTMFPRHKDRVNKKLVDVAKEVAKVDVPAYRRHLDVVVACEDDDGNDVDIPLISIYFR

Target ORF information:

RefSeq Version	XM_015777318.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777318.2

ORF Insert Sequence:

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332529 ( XM_015777316.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632802.1 Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X1, mRNA.

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CloneID	OOa07119
Clone ID Related Accession (Same CDS sequence)	XM_015777317.2 , XM_015777317.1 , XM_015777318.1 , XM_015777318.2 , XM_015777316.1 , XM_015777316.2
Accession Version	XM_015777316.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	ubiquitin-activating enzyme E1 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632802.1
CDS	200..3394
Translation	MLPTKRANGAEAESSSDAPAKKARVGASASEAEAMVAGEAGGGGGGVSGNGSEVAEIDEDLHSRQLAVYGRETMRRLFASNVLVSGLNGLGAEIAKNLALAGVKSITLHDMGNVEMWDLSGNFFLSEDDIGKNRAVACTAKLQELNNAVLISTLTEDLTNEHLSKFQAVVFTDISLDKAFEFDDYCRNHQPSISFIKAEVCGLFGSVFCDFGPKFTVLDVDGEEPHTGIIASISNDNPAMISCVDDERLEFQDGDLVVFSEVQGMTELNDGKPRKIINARPYSFCIQEDTSKFGIYAKGGIVTQVKEPINLEFKSLRDSIREPGNFLLSDFSKFDRPPLLHFAFLALDKFRKEFGRFPGAGCDQDAQRFIEFVASVNEATIDYKMDELDGKLLRNFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPQYQFFYFDSAESLPTYPLDSKDLKPLNSRYDAQISVFGSKLQKKMRDANVFVVGSGALGCEFLKNLALMGVSCGLKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAAAAASAINSSLHINALQNRACPETEHVFHDKFWEGLDVIINALDNVNARMYMDMRCLYFQKPLLESGTLGPKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFISNPAQYAAAMRKAGDAQARELLERVCECLDKERCDGFEDCIAWARLKFEDYFANRVKQLTFTFPEDAVTSTGAFFWSAPKRFPRPLQFSTVNSSHIHFILAASILRAVSFGISIPDWAKNTSNLVDAVSKVVVPEFEPKSGVKIETDEKASNISSASVDDASVIEDLLTKLEASAKKLPPGFQMKAIQFEKDDDTNFHMDLIAGLANMRARNYGIQEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLAGGHPVEDYRNSFANLAIPMFSMAEPLPPKVIKHQDMRWTIWDRWSIEGNITVAELLKWLSDKGLSAYSVSCGTSLLYNTMFPRHKDRVNKKLVDVAKEVAKVDVPAYRRHLDVVVACEDDDGNDVDIPLISIYFR

Target ORF information:

RefSeq Version	XM_015777316.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777316.1

ORF Insert Sequence:

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332529 ( XM_015777316.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632802.1 Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X1, mRNA.

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CloneID	OOa07119
Clone ID Related Accession (Same CDS sequence)	XM_015777317.2 , XM_015777317.1 , XM_015777318.1 , XM_015777318.2 , XM_015777316.1 , XM_015777316.2
Accession Version	XM_015777316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	ubiquitin-activating enzyme E1 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015777316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632802.1
CDS	210..3404
Translation	MLPTKRANGAEAESSSDAPAKKARVGASASEAEAMVAGEAGGGGGGVSGNGSEVAEIDEDLHSRQLAVYGRETMRRLFASNVLVSGLNGLGAEIAKNLALAGVKSITLHDMGNVEMWDLSGNFFLSEDDIGKNRAVACTAKLQELNNAVLISTLTEDLTNEHLSKFQAVVFTDISLDKAFEFDDYCRNHQPSISFIKAEVCGLFGSVFCDFGPKFTVLDVDGEEPHTGIIASISNDNPAMISCVDDERLEFQDGDLVVFSEVQGMTELNDGKPRKIINARPYSFCIQEDTSKFGIYAKGGIVTQVKEPINLEFKSLRDSIREPGNFLLSDFSKFDRPPLLHFAFLALDKFRKEFGRFPGAGCDQDAQRFIEFVASVNEATIDYKMDELDGKLLRNFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPQYQFFYFDSAESLPTYPLDSKDLKPLNSRYDAQISVFGSKLQKKMRDANVFVVGSGALGCEFLKNLALMGVSCGLKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAAAAASAINSSLHINALQNRACPETEHVFHDKFWEGLDVIINALDNVNARMYMDMRCLYFQKPLLESGTLGPKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFISNPAQYAAAMRKAGDAQARELLERVCECLDKERCDGFEDCIAWARLKFEDYFANRVKQLTFTFPEDAVTSTGAFFWSAPKRFPRPLQFSTVNSSHIHFILAASILRAVSFGISIPDWAKNTSNLVDAVSKVVVPEFEPKSGVKIETDEKASNISSASVDDASVIEDLLTKLEASAKKLPPGFQMKAIQFEKDDDTNFHMDLIAGLANMRARNYGIQEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLAGGHPVEDYRNSFANLAIPMFSMAEPLPPKVIKHQDMRWTIWDRWSIEGNITVAELLKWLSDKGLSAYSVSCGTSLLYNTMFPRHKDRVNKKLVDVAKEVAKVDVPAYRRHLDVVVACEDDDGNDVDIPLISIYFR

Target ORF information:

RefSeq Version	XM_015777316.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group ubiquitin-activating enzyme E1 3 (LOC4332529), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777316.2

ORF Insert Sequence:

ATGCTTCCGA CGAAGAGAGC GAACGGCGCT GAGGCTGAGA GCAGCAGCGA CGCGCCGGCG 
AAGAAGGCGC GGGTAGGGGC ATCGGCGTCG GAGGCGGAGG CGATGGTGGC TGGGGAGGCG 
GGCGGCGGTG GGGGTGGGGT GAGCGGCAAT GGGAGCGAGG TGGCGGAGAT CGACGAGGAC 
CTGCACAGCA GGCAGCTCGC CGTGTACGGG AGGGAGACGA TGCGGCGGCT CTTCGCGTCC 
AACGTCCTCG TCTCCGGACT GAACGGGCTT GGGGCTGAGA TTGCGAAGAA TCTTGCTTTA 
GCGGGTGTTA AGTCTATCAC CCTACATGAT ATGGGAAATG TGGAAATGTG GGACTTATCA 
GGCAATTTCT TCCTATCTGA AGATGATATT GGAAAGAATA GGGCTGTTGC TTGCACTGCA 
AAGCTGCAAG AACTCAACAA TGCTGTTCTT ATATCTACTC TGACAGAAGA TCTGACCAAT 
GAACACCTCT CAAAGTTTCA GGCTGTTGTT TTCACTGACA TAAGTCTAGA CAAGGCGTTT 
GAATTTGATG ATTATTGTCG TAACCACCAG CCTTCAATTT CTTTTATCAA AGCAGAAGTT 
TGTGGTCTTT TTGGTAGTGT ATTTTGTGAC TTTGGGCCTA AGTTTACCGT TCTGGATGTT 
GATGGTGAAG AACCACATAC TGGTATAATT GCATCCATCA GCAATGACAA TCCTGCAATG 
ATATCCTGCG TTGATGATGA GCGCCTTGAA TTCCAAGATG GTGATCTTGT TGTCTTCTCA 
GAGGTCCAAG GGATGACAGA ACTAAATGAT GGCAAGCCAA GGAAGATAAT AAATGCAAGG 
CCATATTCAT TTTGCATCCA GGAGGATACA AGTAAATTTG GTATTTATGC AAAAGGTGGA 
ATTGTTACTC AGGTGAAAGA GCCGATCAAC TTAGAGTTCA AGAGTCTCAG AGATTCTATA 
AGAGAACCAG GGAATTTCCT TTTGAGCGAT TTCTCAAAGT TTGACCGTCC TCCTCTTCTG 
CACTTCGCAT TTCTAGCTTT GGATAAGTTC AGGAAAGAAT TTGGGCGCTT TCCTGGTGCT 
GGCTGTGACC AAGATGCTCA AAGGTTTATT GAGTTTGTTG CTTCTGTCAA TGAAGCTACA 
ATTGATTACA AGATGGATGA GCTTGATGGG AAGTTGTTAC GAAATTTTGC AAGTGGTTCT 
AGAGCTGTTC TAAACCCAAT GGCTGCGATG TTTGGTGGCA TTGTCGGCCA AGAAGTTGTT 
AAGGCATGCT CTGGGAAATT TCATCCTCAA TACCAGTTCT TTTATTTTGA TTCAGCGGAA 
TCTCTACCAA CCTATCCATT GGACTCAAAA GACTTGAAAC CATTGAATAG CCGCTATGAT 
GCTCAGATAT CTGTTTTTGG TTCTAAGCTT CAGAAAAAGA TGAGAGATGC CAATGTTTTT 
GTTGTGGGAT CTGGTGCTCT TGGGTGTGAA TTCTTGAAGA ATCTTGCTTT AATGGGAGTA 
TCTTGTGGCC TTAAAGGAAA GCTAACTATA ACAGATGATG ATATCATTGA GAAAAGCAAC 
TTGAGCCGCC AATTTCTGTT TCGTGATTGG AATATTGGGC AGGCTAAATC TACTGTAGCA 
GCTGCAGCGG CTAGTGCTAT CAACTCCAGC CTGCATATTA ATGCTCTTCA GAATCGTGCC 
TGTCCAGAGA CTGAGCATGT GTTCCATGAC AAATTCTGGG AGGGCCTTGA TGTCATCATC 
AATGCACTTG ATAATGTTAA TGCTAGGATG TACATGGATA TGAGATGCTT GTACTTCCAG 
AAACCACTTT TGGAATCTGG AACATTGGGT CCAAAATGCA ATACACAAAT GGTAATTCCT 
CACCTTACTG AAAACTATGG AGCATCCCGA GACCCTCCTG AGAAACAGGC ACCAATGTGC 
ACAGTCCACT CTTTCCCACA CAATATTGAC CACTGTCTGA CATGGGCTCG TTCAGAGTTT 
GAGGGTTTGC TAGAGAAAAC TCCAAATGAA GTGAACTCTT TTATTTCCAA TCCAGCTCAA 
TATGCTGCTG CTATGAGAAA GGCAGGTGAT GCTCAAGCAA GGGAATTGCT CGAGCGTGTT 
TGTGAATGCC TTGACAAGGA GCGATGTGAT GGATTTGAAG ATTGCATTGC CTGGGCTAGA 
CTGAAATTTG AAGACTACTT CGCTAATCGT GTGAAGCAGC TAACATTCAC ATTTCCTGAA 
GATGCGGTCA CTAGCACGGG TGCTTTTTTC TGGTCTGCCC CCAAACGGTT TCCTCGCCCA 
CTGCAATTCT CTACTGTGAA TTCATCTCAC ATTCACTTCA TACTGGCTGC TTCCATATTG 
AGAGCAGTGT CATTCGGGAT CTCCATACCC GACTGGGCAA AGAACACAAG TAATCTGGTC 
GATGCTGTTA GTAAGGTTGT AGTGCCTGAA TTTGAACCGA AGAGTGGGGT TAAGATTGAG 
ACAGATGAGA AAGCTTCTAA CATCTCTAGT GCTTCTGTTG ATGATGCTTC TGTTATTGAA 
GATCTTTTAA CCAAGCTGGA AGCATCTGCA AAAAAATTAC CTCCTGGATT CCAGATGAAG 
GCTATTCAGT TTGAGAAGGA TGATGACACA AACTTCCACA TGGATTTAAT TGCTGGGCTT 
GCAAATATGC GTGCAAGGAA CTATGGTATT CAAGAGGTCG ACAAGCTGAA GGCGAAGTTC 
ATTGCAGGAA GAATCATTCC GGCGATCGCA ACTTCGACAG CCATGGCAAC AGGACTTGTG 
TGCCTTGAGC TTTACAAGGT TCTGGCTGGT GGGCACCCCG TCGAAGACTA CCGCAATTCG 
TTTGCTAACT TGGCAATACC AATGTTCTCC ATGGCTGAAC CTCTTCCACC CAAGGTGATC 
AAGCATCAAG ACATGAGATG GACCATATGG GATCGGTGGT CTATCGAGGG CAACATCACT 
GTCGCAGAAC TCCTCAAGTG GCTGAGTGAC AAGGGCCTGA GCGCGTACAG TGTCTCCTGC 
GGCACTTCAT TGCTTTACAA CACCATGTTT CCGAGACACA AGGATCGAGT GAACAAGAAG 
CTCGTGGATG TCGCCAAGGA GGTGGCGAAG GTGGACGTTC CGGCATACAG GAGGCACCTG 
GATGTCGTCG TTGCCTGTGA AGATGATGAT GGGAATGACG TCGACATCCC TCTCATCTCC 
ATTTACTTCC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC4332529 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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