NCU07516 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU07516
Entrez Gene ID	3874511
Full Name	RING finger membrane protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_953271.3	XP_958364.1	RING finger membrane protein

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_13328	XP_003711185.1
Neurospora crassa	NCU07516	XP_958364.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU07516 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU07516 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf00348	XM_953271.3 Latest version!	Neurospora crassa OR74A RING finger membrane protein (NCU07516), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU07516 ( XM_953271.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_958364.1 Neurospora crassa OR74A RING finger membrane protein (NCU07516), mRNA.

Price & Availability↑

CloneID	ONf00348
Clone ID Related Accession (Same CDS sequence)	XM_953271.3
Accession Version	XM_953271.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	RING finger membrane protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_953271.2.

ATGGGCGACG CTAACCCCGC CGACCCGACC GCTCTTCCGG GCTTCGGCCA GGACCAGCCC 
GACCCTGTCC GATTTCCAAA CACCAGACTG CGAAACTCGC GATCAGCCTC CATCTCGACA 
TGGACCACCA CCTCGGGCGG CGGCCACTCC CACACTCATT CGCAACCCCA CAACAACCCG 
CGCGAATCCG AGCAATGCCG TATCTGCAGA GGCGACGCCT CTCCCGACGA CCCCTTGTAC 
CACCCGTGCA AGTGCAGCGG AAGTATCAAA TGGGTCCATC AGGAATGTCT GATGCAATGG 
CTCGCCCAGA CCCAGAGGAA ACACTGCGAG CTGTGCAAGA CGCCCTTCCG CTTCACCAAG 
CTCTACGATC CCGACATGCC CAGGACCGTC CCCGCGCATA TCTTTGTCGC CCACATGTTC 
AAGTACTCGG TCCGTAAGCT CCTCGCTTGG GCAAGGGCCG CCCTCGTCGT CAGTGTCTGG 
CTCGGGTGGC TCCCCTACTT TATGCGCTCG GTATGGGCCC TGCTGTTCTG GCTCTGTGAG 
GAAGGCTGGG GAGGTCCGGC TGTGTTTTCA AGGTCTCAAA ACACATCATC TTCAAACGGC 
TTCTCAGTCA GGGCCTACGG CACCACCGTA TGCCCAGCTA GTCCACTCTT TGTCGCCAAC 
ACGTCTCCAG CTAGCATGGG GGCCGTGTTG GGACGTCTGC CCCTGAACTC GACCCAGTTC 
GTAAACAGCC TTGCTGGTGC CAGTTCTGGC GCAGGCCATT CCTTCCTTTC CCTTTTGCTT 
CGCACCGTAT TTGGCTCAAG TGGTATCGTG GACAGGCCTT CCCCGCCCCT CAAAGAGATC 
CCCAGTCAGC TCGCTAATAA CGTTACTGCG ACTCTGTACA TCGACCAAGC AGCTTCACCT 
TACAGCAATG CATCATTGGT CCATTCACCT TCGCTTCTCA GCGAGGTCGG CTTTCTAAGG 
AATCTAACTC GGAACCCGAC CGTCAACAAC AACATAATTG CGGTTCTCGA GGGCCAGATC 
ATCACCGTCC TTGTTGTTGT CTGTTTCATC TTGATTATTC TTGTCCGAGA TTACGTTGTT 
CAACAGCAGC CGGAAATCAA CGAGCGCGCC GCTTTTGCCA ACCTTCAAAA CGTGCAGCCC 
CAGGTTGCTC CGCTCAATGT TCCCGGCCCT GCCTTGAACG GTGAACTTGA CGGCGGCCTC 
GGCGAAGAAG AAGGAGAAGA GGAGGATGCA GGTACTGACG ACGAACATGC CTGGGATGCT 
CAGCCTCCCC AGCCTAGACC CCCGCCTGAC AGGCAACCCG TCAACAATCC TTATGCTTCG 
GACTTGGAGA GAGAACTCGG AGAATCGGAC TCATCAACCA TAGATGAGTA CCTCAATCTT 
TACAGAGAGG CCGGCGGAAA CCCAGACAGA ATCGTGGAGC TGGCCCAGGA GCGTGGTTTG 
GAGGAAAGGT TGGCTTACTG GATAGCGCTT ACCAAGACCA TGAAGGAGCG CGCCGCCCGA 
GTTCAGGCGA ATTACAATGA TGGCCTCAAC TCTTCCGCGG CGGAATCCAG TACTAGCGCG 
GCAGCGAGAC CGTCAGGCCA CAACTTGGAG ATGGACAGCA CTCCTGCCGC TCCACGACCC 
GATCCGTTTG ATGACTGGCG CTATCCTTTG GAAGAAGATG ACCATCAGGG TGAAAAAGCC 
GGAGATGGTT CCAGGGACAA GGGCAAGGGC GTTGCGACTG ATGATTCTGA CACCGAGTCG 
GACTCCGAGA TTCTCGCTTC TTTGGCCAGG CCTCGCGCCA ATACAGATGG GCCTGAAATC 
AGAACGTACA CCAATCCTCT GGGAAGCAAC AGCTGGTCTT TCAGCGACTT GCCAGAAGAT 
GACCCAAACA GTTCATGCAC GGAAGAAAAG GCCTCTCAGG TTATTGCAAC GCCCCCAGAT 
CTCACTAATG ACAATGATTC TTCCAACACC GAGGTCGAAC CCTTGCTCGC TTCACTGGCC 
GGTCCAGCAG AATCCGAGTC ATTCCAGGAG AATCGTAGCC AAGATGGCAA TAGGGAGACA 
GCCGCGGATG CCCGTGTACA CATCGGACAG CCCACGGAAG CAGAACACTG GGGAACCAAC 
CTTAACCCAG AGCGTCAGGC GGAACCACAA GCATTGACCG ATGTATCCTG GCCCAATCTC 
GACGAGATCA CGGCAGATAG CCCCGCACAA GCTCCTCAAC CTCGTGGATT CGTCCCTAGA 
GTGACCGACT TCATGTGGCA AGGCGTTGAG GCCGATCAGG CGCAACAGGA TGCTATTGAT 
ATTATCGCTG ACGCTTTGAT GAGACGGGAC GACGAAGACA ACGTGGACGT TCGCGATAAC 
CCTGTACTTG ACAACGATGA TGACGTGGAC CAGGATGATG ACGCCGCAGC AGCAATTGGC 
CTCGAACAAG ACGCCATTGA TGATGCCGAA GACCTCGAAG GCGTCCTGGA GCTTCTTGGC 
ATGCGCGGGC CTCTGGCTGG CCTATTCCAA AACGCCATCT TCTGCGTCCT GCTCGTGTCA 
GCTACCATTT TTCTCGGACT GTTCATTCCA TACAACATCG GTCGCTATTC GCTGTGGTTC 
ATTGCGAAGC CTATGCGTCC AGTCTGGATT CTCTACAGTC TGTGCAGGTT CATTCAGGAC 
ATGGTTGTCA TTGCCTTCGG GCTCGTTTCC CACGCGCTAT CTCTGGTGCA TCCCAGTCTA 
GGGCTGGGTG GTGTGGCTCG CCTGGCCACG AAGCTCCTCT TCGGTAGCCG CCACGCTGCA 
GCGATTCTCG AACCACATAT TGAAATGACC AACGCCGCTA GGCGAGTTGG ACACACGGTC 
ATCAACGAGC TCGGCCGCGT ATCCATGTCA GAGATTCATA CCTTTTCTGC GGTTAGTCAT 
GAAGCCTTAT TGTCGGTCAA GGGGCAAGTC TTTTCCGGCT TTGCCGCCCT GGGCTCCGCG 
CTTGTCTTTC TTTTTGGAGG GGATTACTCT ACCAAAGCTC CTATTGTGCT CTCTGCTGCG 
AGCAACGCGA CTACATTCGC GTTGGGCGCA CTCAAGACTC TGCCGACTGC CTTATTGAAG 
CCTAACCTGG TCATCAATGT CAACTTCCCT GACGCGAGTC ATACCATCGA CCCGGCGTTG 
GCATACTGGA ATGGCACAGA CAGATTCTGG GCAATCCTGG CTGGCTACCT CACAATTTAC 
CTTGCGGCGG TCTTGTACCT GCGTCATGGC GGACCCATAT CCTCCAGCCC AACCGGACAG 
GAATGGGAGG CAGCTCTTAT TGACGCACTC AACCAGGCAA GCGGTGTGAT GAAGGTCATC 
CTTATTATTG GCATCGAGAT GCTCGTCTTC CCGCTTTACT GCGGCATGCT TTTGGACATT 
GCACTCTTGC CCTTGTTCGA AAATGCCACC CTCATGTCTC GCCTTCAATT CACCCTCAAC 
TTCCCCATGA CATCGATCTT CGTCCACTGG TTCGTGGGTA CGGCTTACAT GTTCCACTTT 
GCGCTGTTCG TCTCTATGTG CCGCAAGATC ATGCGCCGTG GAGTTCTCTA CTTCATCCGC 
GACCCAGACG ATCCCGAGTT CCACCCGGTA CGCGACGTAC TGGAGCGCAA CGTCACGACC 
CAGTTACGGA AGATTGCCTT CTCTGCTCTC GTCTACGGGG CGCTTGTCAT AATCTGTCTG 
GGTGCTGTGG TCTGGGGGTT GGCGCTCTCT TTGCCCAATG TCCTGCCGAT TCACTACTCG 
TCCAACGAGC CAGTGCTCGA GTTCCCGCTT GATCTTCTCT TTTACAACTT CCTGATGCCC 
TTGGCCGTCA AGTTCTTCAA GCCTGGTGAT GGACTTCATG CTATGTACAC TTGGTGGTTC 
CGCACTTGCG GCCGGTGCTT GCGAATTACT TGGTTTCTGT TCGGGGAGCG CAAGGCAGAT 
GAAGAAGGCC GTCTGCGCAT GATTGAGAGA GGCCCCGATG CCCAGGTATC ATGGTGGCAG 
GCTATGTTCC TTCAATTGGA TGAGTCTAAT GGGATTGTGA GACCGTGGAA GCCCAAGTTC 
CTGGCCGAAA GCACCAAGGC TGAGACTTCC GAGTCCGAAC CTGAATCACC GAATATCCGA 
GGGTATGTGC CGTCTGAGGC TGAGGCTGCT ACCAGGAAAC TGTCTCCTGA AGAAGGCTTC 
AAGGATTACT TGGTCAATAC CAAGCAACTG ATTCCTGATG GCCGCTTCGT TCGCGCTCCG 
GCATCTGACC AGGTCAAGAT TCCCAAGGGC AAGACGGTGT TTCTTGAGGT GACGGAGAGG 
AATGAGAGAC TTGACGGCAA GCCTGATGCA CCCGAGACCG ACCTTTACTC GACCACGCAG 
TACCGCCTGG TCTATGTCCC ACCGCACTTC CGAATCCGCA TCTTCTTCTT CATTCTCCTC 
ATCTGGTTGT TTGCCGCCGC TACGGGCGTC GGCATTACGA TTATCCCCTT GGTCTTTGGC 
CGCTGGATGT TCAAACTCTT GATTCCGGAG GGCGTAAGGA CAAATGACAT TTACGCCTTC 
GGCATCGGCA TCCACGTCCT GGGGGTGATT GTTTACGCGC TCATCAACAT CCGGTCCCTT 
TCTCAGACTG CCAAGATCAG GGTCGCCGCG ACTATCGCGT CAGTCTTTAA CAACGGCGCA 
GTCCGCCGCA GCCTGGCTAT CATCACCCGC ATCGCCAAGA TTATCTACTC CTACGCCTTC 
CTCCTGGTCG TCTTCCCCCT CATGATCGCC TCCGTCGCCG AGCTCTACAT CATGATGCCC 
CTGCACGAAT TCATGTACAG CGTCCCCATC GCCCAAGACA TCAAGCAGGT CACCATCCAA 
CACGCGGCCG CTCTCAACCC GCGCCACACC ATCCGCGTCA TGCAGTCCTG GACCATTGGT 
CTTTTGTACT TGAAGCTTTC GGTAAGGGGA ATCACCACCT GGTTCAGCGG CAGCCGTCTG 
GCCCTCGCTA CCAAGGCCGT GCTGCGCCGT GGCTGGTTCA ACCCCAACGC CGGGGTCTTG 
ACTCGCGCTT TCGTCATCCC TGCCATCCTC CTCTGGACCG TTGCTATGGC TATTCCTACC 
CTCTTCGCCA AGTTTATGAT CTCCTGGAGC CTGGCAGAGA CCTTGACTCT CCGCCATCCT 
CAAGCCGTCA ACGTCGACGG CGTGGTGGAC CAGACTCTCT ACGACGCCTA CGTCGTGCTC 
ATCTACCGGC GCAGCTTCCC GCTTACAGCA CTGTTGTTGT TGGCTGCGGC GGCGGTGTGG 
GGCCTGCTGG GTGTGTTCCA GAGTTGGAAG ATGAGGATCA GGGATGAGGC GTATCTCATT 
GGGGAGCGGC TGCAGAATTA CGGAGGTGGG ATGCCGCAGA GGGGACTTGG AAGGGGGACG 
AGGGGTGGAT GGAGAGCTGG GATGGCTGTT GGAGGGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_958364.1
CDS	585..5966
Translation	MGDANPADPTALPGFGQDQPDPVRFPNTRLRNSRSASISTWTTTSGGGHSHTHSQPHNNPRESEQCRICRGDASPDDPLYHPCKCSGSIKWVHQECLMQWLAQTQRKHCELCKTPFRFTKLYDPDMPRTVPAHIFVAHMFKYSVRKLLAWARAALVVSVWLGWLPYFMRSVWALLFWLCEEGWGGPAVFSRSQNTSSSNGFSVRAYGTTVCPASPLFVANTSPASMGAVLGRLPLNSTQFVNSLAGASSGAGHSFLSLLLRTVFGSSGIVDRPSPPLKEIPSQLANNVTATLYIDQAASPYSNASLVHSPSLLSEVGFLRNLTRNPTVNNNIIAVLEGQIITVLVVVCFILIILVRDYVVQQQPEINERAAFANLQNVQPQVAPLNVPGPALNGELDGGLGEEEGEEEDAGTDDEHAWDAQPPQPRPPPDRQPVNNPYASDLERELGESDSSTIDEYLNLYREAGGNPDRIVELAQERGLEERLAYWIALTKTMKERAARVQANYNDGLNSSAAESSTSAAARPSGHNLEMDSTPAAPRPDPFDDWRYPLEEDDHQGEKAGDGSRDKGKGVATDDSDTESDSEILASLARPRANTDGPEIRTYTNPLGSNSWSFSDLPEDDPNSSCTEEKASQVIATPPDLTNDNDSSNTEVEPLLASLAGPAESESFQENRSQDGNRETAADARVHIGQPTEAEHWGTNLNPERQAEPQALTDVSWPNLDEITADSPAQAPQPRGFVPRVTDFMWQGVEADQAQQDAIDIIADALMRRDDEDNVDVRDNPVLDNDDDVDQDDDAAAAIGLEQDAIDDAEDLEGVLELLGMRGPLAGLFQNAIFCVLLVSATIFLGLFIPYNIGRYSLWFIAKPMRPVWILYSLCRFIQDMVVIAFGLVSHALSLVHPSLGLGGVARLATKLLFGSRHAAAILEPHIEMTNAARRVGHTVINELGRVSMSEIHTFSAVSHEALLSVKGQVFSGFAALGSALVFLFGGDYSTKAPIVLSAASNATTFALGALKTLPTALLKPNLVINVNFPDASHTIDPALAYWNGTDRFWAILAGYLTIYLAAVLYLRHGGPISSSPTGQEWEAALIDALNQASGVMKVILIIGIEMLVFPLYCGMLLDIALLPLFENATLMSRLQFTLNFPMTSIFVHWFVGTAYMFHFALFVSMCRKIMRRGVLYFIRDPDDPEFHPVRDVLERNVTTQLRKIAFSALVYGALVIICLGAVVWGLALSLPNVLPIHYSSNEPVLEFPLDLLFYNFLMPLAVKFFKPGDGLHAMYTWWFRTCGRCLRITWFLFGERKADEEGRLRMIERGPDAQVSWWQAMFLQLDESNGIVRPWKPKFLAESTKAETSESEPESPNIRGYVPSEAEAATRKLSPEEGFKDYLVNTKQLIPDGRFVRAPASDQVKIPKGKTVFLEVTERNERLDGKPDAPETDLYSTTQYRLVYVPPHFRIRIFFFILLIWLFAAATGVGITIIPLVFGRWMFKLLIPEGVRTNDIYAFGIGIHVLGVIVYALINIRSLSQTAKIRVAATIASVFNNGAVRRSLAIITRIAKIIYSYAFLLVVFPLMIASVAELYIMMPLHEFMYSVPIAQDIKQVTIQHAAALNPRHTIRVMQSWTIGLLYLKLSVRGITTWFSGSRLALATKAVLRRGWFNPNAGVLTRAFVIPAILLWTVAMAIPTLFAKFMISWSLAETLTLRHPQAVNVDGVVDQTLYDAYVVLIYRRSFPLTALLLLAAAAVWGLLGVFQSWKMRIRDEAYLIGERLQNYGGGMPQRGLGRGTRGGWRAGMAVGGR

Target ORF information:

RefSeq Version	XM_953271.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A RING finger membrane protein (NCU07516), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_953271.3

ORF Insert Sequence:

ATGGGCGACG CTAACCCCGC CGACCCGACC GCTCTTCCGG GCTTCGGCCA GGACCAGCCC 
GACCCTGTCC GATTTCCAAA CACCAGACTG CGAAACTCGC GATCAGCCTC CATCTCGACA 
TGGACCACCA CCTCGGGCGG CGGCCACTCC CACACTCATT CGCAACCCCA CAACAACCCG 
CGCGAATCCG AGCAATGCCG TATCTGCAGA GGCGACGCCT CTCCCGACGA CCCCTTGTAC 
CACCCGTGCA AGTGCAGCGG AAGTATCAAA TGGGTCCATC AGGAATGTCT GATGCAATGG 
CTCGCCCAGA CCCAGAGGAA ACACTGCGAG CTGTGCAAGA CGCCCTTCCG CTTCACCAAG 
CTCTACGATC CCGACATGCC CAGGACCGTC CCCGCGCATA TCTTTGTCGC CCACATGTTC 
AAGTACTCGG TCCGTAAGCT CCTCGCTTGG GCAAGGGCCG CCCTCGTCGT CAGTGTCTGG 
CTCGGGTGGC TCCCCTACTT TATGCGCTCG GTATGGGCCC TGCTGTTCTG GCTCTGTGAG 
GAAGGCTGGG GAGGTCCGGC TGTGTTTTCA AGGTCTCAAA ACACATCATC TTCAAACGGC 
TTCTCAGTCA GGGCCTACGG CACCACCGTA TGCCCAGCTA GTCCACTCTT TGTCGCCAAC 
ACGTCTCCAG CTAGCATGGG GGCCGTGTTG GGACGTCTGC CCCTGAACTC GACCCAGTTC 
GTAAACAGCC TTGCTGGTGC CAGTTCTGGC GCAGGCCATT CCTTCCTTTC CCTTTTGCTT 
CGCACCGTAT TTGGCTCAAG TGGTATCGTG GACAGGCCTT CCCCGCCCCT CAAAGAGATC 
CCCAGTCAGC TCGCTAATAA CGTTACTGCG ACTCTGTACA TCGACCAAGC AGCTTCACCT 
TACAGCAATG CATCATTGGT CCATTCACCT TCGCTTCTCA GCGAGGTCGG CTTTCTAAGG 
AATCTAACTC GGAACCCGAC CGTCAACAAC AACATAATTG CGGTTCTCGA GGGCCAGATC 
ATCACCGTCC TTGTTGTTGT CTGTTTCATC TTGATTATTC TTGTCCGAGA TTACGTTGTT 
CAACAGCAGC CGGAAATCAA CGAGCGCGCC GCTTTTGCCA ACCTTCAAAA CGTGCAGCCC 
CAGGTTGCTC CGCTCAATGT TCCCGGCCCT GCCTTGAACG GTGAACTTGA CGGCGGCCTC 
GGCGAAGAAG AAGGAGAAGA GGAGGATGCA GGTACTGACG ACGAACATGC CTGGGATGCT 
CAGCCTCCCC AGCCTAGACC CCCGCCTGAC AGGCAACCCG TCAACAATCC TTATGCTTCG 
GACTTGGAGA GAGAACTCGG AGAATCGGAC TCATCAACCA TAGATGAGTA CCTCAATCTT 
TACAGAGAGG CCGGCGGAAA CCCAGACAGA ATCGTGGAGC TGGCCCAGGA GCGTGGTTTG 
GAGGAAAGGT TGGCTTACTG GATAGCGCTT ACCAAGACCA TGAAGGAGCG CGCCGCCCGA 
GTTCAGGCGA ATTACAATGA TGGCCTCAAC TCTTCCGCGG CGGAATCCAG TACTAGCGCG 
GCAGCGAGAC CGTCAGGCCA CAACTTGGAG ATGGACAGCA CTCCTGCCGC TCCACGACCC 
GATCCGTTTG ATGACTGGCG CTATCCTTTG GAAGAAGATG ACCATCAGGG TGAAAAAGCC 
GGAGATGGTT CCAGGGACAA GGGCAAGGGC GTTGCGACTG ATGATTCTGA CACCGAGTCG 
GACTCCGAGA TTCTCGCTTC TTTGGCCAGG CCTCGCGCCA ATACAGATGG GCCTGAAATC 
AGAACGTACA CCAATCCTCT GGGAAGCAAC AGCTGGTCTT TCAGCGACTT GCCAGAAGAT 
GACCCAAACA GTTCATGCAC GGAAGAAAAG GCCTCTCAGG TTATTGCAAC GCCCCCAGAT 
CTCACTAATG ACAATGATTC TTCCAACACC GAGGTCGAAC CCTTGCTCGC TTCACTGGCC 
GGTCCAGCAG AATCCGAGTC ATTCCAGGAG AATCGTAGCC AAGATGGCAA TAGGGAGACA 
GCCGCGGATG CCCGTGTACA CATCGGACAG CCCACGGAAG CAGAACACTG GGGAACCAAC 
CTTAACCCAG AGCGTCAGGC GGAACCACAA GCATTGACCG ATGTATCCTG GCCCAATCTC 
GACGAGATCA CGGCAGATAG CCCCGCACAA GCTCCTCAAC CTCGTGGATT CGTCCCTAGA 
GTGACCGACT TCATGTGGCA AGGCGTTGAG GCCGATCAGG CGCAACAGGA TGCTATTGAT 
ATTATCGCTG ACGCTTTGAT GAGACGGGAC GACGAAGACA ACGTGGACGT TCGCGATAAC 
CCTGTACTTG ACAACGATGA TGACGTGGAC CAGGATGATG ACGCCGCAGC AGCAATTGGC 
CTCGAACAAG ACGCCATTGA TGATGCCGAA GACCTCGAAG GCGTCCTGGA GCTTCTTGGC 
ATGCGCGGGC CTCTGGCTGG CCTATTCCAA AACGCCATCT TCTGCGTCCT GCTCGTGTCA 
GCTACCATTT TTCTCGGACT GTTCATTCCA TACAACATCG GTCGCTATTC GCTGTGGTTC 
ATTGCGAAGC CTATGCGTCC AGTCTGGATT CTCTACAGTC TGTGCAGGTT CATTCAGGAC 
ATGGTTGTCA TTGCCTTCGG GCTCGTTTCC CACGCGCTAT CTCTGGTGCA TCCCAGTCTA 
GGGCTGGGTG GTGTGGCTCG CCTGGCCACG AAGCTCCTCT TCGGTAGCCG CCACGCTGCA 
GCGATTCTCG AACCACATAT TGAAATGACC AACGCCGCTA GGCGAGTTGG ACACACGGTC 
ATCAACGAGC TCGGCCGCGT ATCCATGTCA GAGATTCATA CCTTTTCTGC GGTTAGTCAT 
GAAGCCTTAT TGTCGGTCAA GGGGCAAGTC TTTTCCGGCT TTGCCGCCCT GGGCTCCGCG 
CTTGTCTTTC TTTTTGGAGG GGATTACTCT ACCAAAGCTC CTATTGTGCT CTCTGCTGCG 
AGCAACGCGA CTACATTCGC GTTGGGCGCA CTCAAGACTC TGCCGACTGC CTTATTGAAG 
CCTAACCTGG TCATCAATGT CAACTTCCCT GACGCGAGTC ATACCATCGA CCCGGCGTTG 
GCATACTGGA ATGGCACAGA CAGATTCTGG GCAATCCTGG CTGGCTACCT CACAATTTAC 
CTTGCGGCGG TCTTGTACCT GCGTCATGGC GGACCCATAT CCTCCAGCCC AACCGGACAG 
GAATGGGAGG CAGCTCTTAT TGACGCACTC AACCAGGCAA GCGGTGTGAT GAAGGTCATC 
CTTATTATTG GCATCGAGAT GCTCGTCTTC CCGCTTTACT GCGGCATGCT TTTGGACATT 
GCACTCTTGC CCTTGTTCGA AAATGCCACC CTCATGTCTC GCCTTCAATT CACCCTCAAC 
TTCCCCATGA CATCGATCTT CGTCCACTGG TTCGTGGGTA CGGCTTACAT GTTCCACTTT 
GCGCTGTTCG TCTCTATGTG CCGCAAGATC ATGCGCCGTG GAGTTCTCTA CTTCATCCGC 
GACCCAGACG ATCCCGAGTT CCACCCGGTA CGCGACGTAC TGGAGCGCAA CGTCACGACC 
CAGTTACGGA AGATTGCCTT CTCTGCTCTC GTCTACGGGG CGCTTGTCAT AATCTGTCTG 
GGTGCTGTGG TCTGGGGGTT GGCGCTCTCT TTGCCCAATG TCCTGCCGAT TCACTACTCG 
TCCAACGAGC CAGTGCTCGA GTTCCCGCTT GATCTTCTCT TTTACAACTT CCTGATGCCC 
TTGGCCGTCA AGTTCTTCAA GCCTGGTGAT GGACTTCATG CTATGTACAC TTGGTGGTTC 
CGCACTTGCG GCCGGTGCTT GCGAATTACT TGGTTTCTGT TCGGGGAGCG CAAGGCAGAT 
GAAGAAGGCC GTCTGCGCAT GATTGAGAGA GGCCCCGATG CCCAGGTATC ATGGTGGCAG 
GCTATGTTCC TTCAATTGGA TGAGTCTAAT GGGATTGTGA GACCGTGGAA GCCCAAGTTC 
CTGGCCGAAA GCACCAAGGC TGAGACTTCC GAGTCCGAAC CTGAATCACC GAATATCCGA 
GGGTATGTGC CGTCTGAGGC TGAGGCTGCT ACCAGGAAAC TGTCTCCTGA AGAAGGCTTC 
AAGGATTACT TGGTCAATAC CAAGCAACTG ATTCCTGATG GCCGCTTCGT TCGCGCTCCG 
GCATCTGACC AGGTCAAGAT TCCCAAGGGC AAGACGGTGT TTCTTGAGGT GACGGAGAGG 
AATGAGAGAC TTGACGGCAA GCCTGATGCA CCCGAGACCG ACCTTTACTC GACCACGCAG 
TACCGCCTGG TCTATGTCCC ACCGCACTTC CGAATCCGCA TCTTCTTCTT CATTCTCCTC 
ATCTGGTTGT TTGCCGCCGC TACGGGCGTC GGCATTACGA TTATCCCCTT GGTCTTTGGC 
CGCTGGATGT TCAAACTCTT GATTCCGGAG GGCGTAAGGA CAAATGACAT TTACGCCTTC 
GGCATCGGCA TCCACGTCCT GGGGGTGATT GTTTACGCGC TCATCAACAT CCGGTCCCTT 
TCTCAGACTG CCAAGATCAG GGTCGCCGCG ACTATCGCGT CAGTCTTTAA CAACGGCGCA 
GTCCGCCGCA GCCTGGCTAT CATCACCCGC ATCGCCAAGA TTATCTACTC CTACGCCTTC 
CTCCTGGTCG TCTTCCCCCT CATGATCGCC TCCGTCGCCG AGCTCTACAT CATGATGCCC 
CTGCACGAAT TCATGTACAG CGTCCCCATC GCCCAAGACA TCAAGCAGGT CACCATCCAA 
CACGCGGCCG CTCTCAACCC GCGCCACACC ATCCGCGTCA TGCAGTCCTG GACCATTGGT 
CTTTTGTACT TGAAGCTTTC GGTAAGGGGA ATCACCACCT GGTTCAGCGG CAGCCGTCTG 
GCCCTCGCTA CCAAGGCCGT GCTGCGCCGT GGCTGGTTCA ACCCCAACGC CGGGGTCTTG 
ACTCGCGCTT TCGTCATCCC TGCCATCCTC CTCTGGACCG TTGCTATGGC TATTCCTACC 
CTCTTCGCCA AGTTTATGAT CTCCTGGAGC CTGGCAGAGA CCTTGACTCT CCGCCATCCT 
CAAGCCGTCA ACGTCGACGG CGTGGTGGAC CAGACTCTCT ACGACGCCTA CGTCGTGCTC 
ATCTACCGGC GCAGCTTCCC GCTTACAGCA CTGTTGTTGT TGGCTGCGGC GGCGGTGTGG 
GGCCTGCTGG GTGTGTTCCA GAGTTGGAAG ATGAGGATCA GGGATGAGGC GTATCTCATT 
GGGGAGCGGC TGCAGAATTA CGGAGGTGGG ATGCCGCAGA GGGGACTTGG AAGGGGGACG 
AGGGGTGGAT GGAGAGCTGG GATGGCTGTT GGAGGGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU07516 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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