NCU04350 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU04350
Entrez Gene ID	3872478
Full Name	chitin synthase 6
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_951238.3	XP_956331.3	chitin synthase 6

Pathways from BioSystems

Homologies

Neurospora crassa	NCU04350	XP_956331.2
Magnaporthe oryzae (rice blast fungus)	MGG_13014	XP_003712158.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU04350 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU04350 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10011	XM_951238.3 Latest version!	Neurospora crassa OR74A chitin synthase 6 (NCU04350), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU04350 ( XM_951238.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956331.3 Neurospora crassa OR74A chitin synthase 6 (NCU04350), mRNA.

Price & Availability↑

CloneID	ONf10011
Clone ID Related Accession (Same CDS sequence)	XM_951238.3
Accession Version	XM_951238.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chitin synthase 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_951238.2.

ATGGCGAACC GCATGTCAAT GTATTCCATG GCCTCGGACG CCGGTATGGG CGGACGGGGT 
CCTGGTCAGC AACAGCCATC CCAGGTCTCT ACCACGACTC TTCTCAACTC CGTACACAAC 
ATCTACCTCT CTTCACAACC ATACCGTCTG GACGCCGGCA CAAGTCTCGT CGTCAACACA 
TGGCTTACGG CCTCCCAAGC TGGTCCCGAT GGCCACGTTG GTGGAACCGT TGATGGCGCT 
CTGGCTTTGA GGGCATGGGA GCACGCTCGT CGCCGTGCTG AGGATGGATG TATCATTCTT 
GGATCACTGC ACAACTCGAC TCCTTCGCTC TTGCGACCCT TCCTGTCCAC CATTCCCCTT 
GAGATCCCTG CCTCGGTGTA CAAAGCACTC GATGCTATTG AACCGTTCCT TCGCTGTGTA 
ACCCCTTACA ACCCCTCCAC TCCGAGACAG ACCGGCTTGG CCGTCACCTT GACCCTCAAC 
CTTACAGGCA ACCTTACTGC CGCCTCACTC TCGCTCACAC AAGGTGGTAT CGACACCGAC 
AAAGGCTTGT TGGGTGTCCC CTCTGAGCCT GGATACAGGG CCTTTGATGT CTTTTACTAC 
CTGCTCACCA CGGCCTCTAC CCCCGCCGAA AGAGAGTTCC TTGGGCTCAA GGCCGCGTCT 
CAATACAGTC TTCTCGCCCG GTCCGGCACC TACGATCCCC CATCCTATCT TCCTACCGCC 
GATGATGGTG CCGCTGCCGA CGACTTCAGG TCTGCCCTCA AGGACATCGG CATCAAGGGC 
TCTTCGCACC GCAACCTCAT CTCGATCTTG GCCGGCCTTC TCAAGCTCGG CAACACACTC 
GATTACAATG TTGACCAAGA AGTTTTGGAG GAAATCTGCG AGGATGTCGG TGGACTTCTT 
GGTATCGAAC CCGAAATTCT TGCCAGGCAA CTCAGCACCG ACGACCGGGC GACATTGATC 
GGAGGACTCT ATGAGGCTTT GGTCGATTGG GTCATCAAGA AGGCCAACGA TGCTATTGCC 
GTGCAGATGG GTAAATCCAG AAGTGGCGAT GATTCTAGCG ACGGTGCCTC CCGGAGTGGT 
GCGCCCACTT CCAACGAGGA CAGTGGCGAT ACTGTCTGCC TTACTGTCCT CGAGATTCCC 
GACCAAAACT TGGGCAAGGC TATTGCCATG CGGGGCATCT TCGACGACAC TCAGGGTATC 
AACTCGGAAA TGAAGGCTGA CGGCGTCGAG GTGGTTCCTG TTTCCAGCTC GGTTGTGCGG 
GAAATGCAGA GTGCTGTTGC AGAGTGTGGT CCTGATCTGG GTGTTATGGT TGGCTCGGTT 
GGCCGGGAAA GGCAACGCCT GATTGAGAAG CGCGAGGAAA TCTTGGAAAA GACTGCCTTC 
GCATCAGAAG ATGAGGGTTT CTTGAGGAAG CTCTTGCTCC CGGTTCCCAC CGAGGGTATC 
AACCTCGGCC GCACTGGCCG TGTGGATGTT GCGGATCTTT TGAACCGCAG TCGTGCTTGG 
TATCACCTCT GCATCCACCC CACAGACGAC TCACCTTCTA GTCTCGCTGC CCTTCCTGCC 
GTCAACTCAG CCTGGTCAGC TGGAGCTGTT TCTCGCCAGC TCCGCACCTG GAGGCTGTCC 
GAGTGGGCCA ACAGACGCAA TAAGCACATC GACTACACCG CCGATTTTGA TCACCATGAA 
TTTACTCAGC GATATCGGCC TCTGGGCTGT ATGGATGGCC GTGACGGTAT CGAGAGCTGG 
ATCCTGGAGC GGGGCTGGAG CAACGGCGAG GTTGTCGTGG GCAACGAGAG GGTATGGATG 
AGGGAGAGCG CGTGGTGGGA AGCCGAAAGT ATGTTGGACC TGAAGCCCAT GGAAGCTGAG 
AGGCTCAACA GCATGCGCAA CCTGATGGCC CCAGGGGCCA TGGGCTCGGG CTATTCTCAC 
AACGGAAGCG GCTTCTTCCC GCCTCAGGTA CATGCTGACC ACATGCGTTC TCCCTTTGCC 
GATGGAACCT CCAACGGGAG CTACGATCAC CTCGCCCTCG GTGGTAATCT TAGCCAAAAC 
AACCTCAACC CGAGCCCCGC GCGTGCTCCT TCCGTTGCCC CTACGAACAT GACTGGCATG 
CGTAGCGGTG ATTACGGTCT TGGTCCCAAG GGCGATCAAT ACAAGGGACA GGTCTTCTAC 
AACGCTGAAG GCCAACTGGT TTCGGAATTA GAGCCCGACC TTGCTGAGGG CAAGAAGATC 
GAGGAGCGGC CTATCTCCTC CCAAAGGAAA ATTTGGGTCT TTACTGTTTG GGCTTTGACG 
TGGTGGATTC CATCTCCATT ACTCAAGTTC GTCGGTCGGA TGAAGCGGCC TGACGTGCGT 
ATGGCATGGC GTGAGAAGTT TGTTCTCTGC CTCATTATCG TTCTTCTCAA CGCCATCATC 
GTTTTCTGGA TTATCTTCTT CGGTCGTCTT CTTTGCCCCA ATTTCGACAA AGCCTGGACT 
CTGGAAGAAA TTCAGAATCA TGCGGCAGGG GATGACTTCT GGGTCAGCAT TGACGGCAAG 
GTTTACGACA TCACCAAATT CTGGAGATTG CAGCACAGCG ACAACAACAT TGACACCACG 
TCGGATGTAA TGATGCCGCT TGCTGGCCAA GACCTCTCCG CTTACTTTGT CGCGCCTCTC 
TACATTACCT GCCCCGGCCT CGGCATCGCG GATTCAACTA CGTTGCAATT CAACACTACC 
CCTGAATATG TTGTGGCCCA GCATATCTCC GGCCCCACGC AGCCTAATAT TCGGAGCAAG 
CTCTCTGAAA GATACTGGTA TACCGAGAGA TTCTTGCCTG CCATCAAGGA GTACTACCAT 
GGCGACGTGG TTTGGGATTC CAAGAAGATT AAGCAGGAAG GCCTGGATTG GAATCACCCG 
TGGTTCATCT ACGAGGATCT CGTTTACGAC ATGACCGATT ACATGTACAC CGCCAAGCGT 
ATGAATAACG ATGCTACATA CACGTTCATC GACGCCAAGA TCACCAACAT GGTCAAGAAC 
AACCCCGGCC AGGACTTGAC CGAGCAGATT AACGACCTGA TTGATCGCGC CAGTAGCAAC 
ACGACCCAGT ATAATAGTGT TATGAACAGT CTTAACTGTG TCAAGAACGC GTTCTACGTT 
GGCAAGCCCG ATTTCCGTTA TTCGGCCAGG TGTCAAGTCA ACAACTACAT CTTGCTTGCA 
TTTTCCGTTA TTATCTGCAG TATCATCGTG GTCAAGTTCG TGTCGGCTCT TCAATTTGGA 
TCCAAGAGAC GCCCTGCTCC CCAGGACAAG TTTGTCATCT GCCAGGTGCC CGCTTATACC 
GAGGGTGAAG ACTCGCTGAG GAAAGCCCTG GACTCTTTGA CAGCCCTCCA GTACGATAAC 
AAGCGGAAGC TGATCTTTGT TATTTGCGAC GGTGTCATCG TCGGTGCTGG CAACGACCGT 
CCTACTCCCA AGATCGTCCT CGATATTCTC GGTGTTGACC CCAAGGTTGA CCCACCTGCC 
TTGGCCTTCA AATCCGTCGG TACTGGAGCC GAACAGCTCA ACTACGGCAA GGTCTACTCT 
GGATTGTATG AATTTGAAGG GAACGTGGTA CCTTATATCG TGGTTGTCAA GGTCGGCAAG 
GAGTCTGAGC AGTCCAAGAC GAAGCCCGGT AACCGTGGTA AGCGTGACTC GCAGATTCTG 
CTCATGAGCT TCCTCAACCG CGTCCATCAC CGTGCGCCCA TGAATCCCTT GGAACTGGAA 
ATCTTCCACC AGATCAACAA CATCATCGGT GTGGATCCCG AACTTTACGA ATACCTCCTC 
ATGGTCGATG CCGATACCTG CGTTCGCGAA GATTCTCTCA ACCGCCTAGT GGCAGCCTGC 
GCCAATAACG CCAAGATTGC CGGTATTTGC GGTGAAACCA GTTTGCAGAA TGAGGATCTT 
TCGTGGTGGA CCATGATCCA GGTTTACGAG TACTATATTT CTCATCACTT AGCGAAGGCT 
TTCGAGTCTC TCTTTGGCAG CGTTACCTGT TTGCCCGGTT GTTTCACCAT GTACCGCTTG 
CGTACTGTTG ACAAGGGAAA GCCGCTCATT ATCTCGGATG ATATTATCCG CGAGTACAGC 
GATTGCTATG TTGACACCCT CCACAAGAAG AACCTGCTCT CGCTTGGTGA GGATCGTTTC 
CTCACCACGC TCATGACCAA GCACTTCCCA TACATGTCTT ACAAGTTCAT TCCCGATGCC 
TACTGCCAAA CGGCCGCCCC CGAGACTTGG AGCGTTCTGC TGTCTCAGCG TCGTCGTTGG 
ATCAACTCAA CCATCCACAA CTTGGCTGAA TTGATCTTCC TCGACGAAAT GTGCGGTTTC 
TGTTTCTTCT CAATGCGCAT TGTCGTCTTC ATCGATCTCT TCGGTACTAT TATTCTTCCC 
GCCACTTGCG TCTACATCGT TTATCTCATT TACACGGCCT CCTCCCACAC TGCACCGTTC 
CCTCTTCTTT CGATCATCAT GATTGCGGCC GTGTACGGTC TCCAGGCGCT CATCTTCATC 
CTCAAGCGCC AATGGCAGCA CGTGGGCTGG ATGATCATCT ACCTCCTCGC CTTCCCCATT 
TACTCCTTCA TCCTGCCCAT CTACTCGTTC TGGCATCAGG ATGACTTCGC CTGGGGTAAT 
ACGCGCGTTG TCATTGGTGA AAGCGGTAAA AAGCAAGTTG TCGCCGTCGA CGAGGAGGGC 
TTCAACCCCC GTGCCATTCC TCTCCAGCGC TGGGACGAAT ATGCCCTCGC CAACAACCTT 
CCCGGTCGTC GCGGTGGCCC TCTTGAGAAG GGTATGGACG AGCATCTGGG CGGTATGTAC 
GAGGAGACCT ACGAGGTCGA TGACATGAAG TCAGTCTACT CTTCGGTCCG CCAACCGTCC 
GTTCTTACTG GGTTCGGCGG TCGCGGTGCC GGAGCTTACA TGCCCCCTCA CAGCCCTGGT 
TTCCCGCCCA ACGGCGGCGG TAGCGGCGCC ATGACCCATG CCAGCACCTT TGTCGGCGCC 
TCCCCTTTCA CCGAGCAGAA CAACCTCATG AACCGTCAGA GCATGGCCTC GATGGGCACC 
ATGGTCGAGA TGCAGCGCCG CGCCAGCCCG TACCAGGACT TCCCTGGCGG TCATAGCCGC 
GGCCCCAGCC AGGGTGCTAT GCGCTCCATG ACCAATACGC CTCCCGCTCT CGGCGCCGGC 
GGCATGGTCA ACGCCCGCCT GTCCACGGCC ACGGGCATGA GCCTGGGTAT TCCTGGACAC 
CGTCCCGGCA TGAGCCACTC GGAAAGTTCG CGGTCGTTTG ACTTTCAGCG CAGCACTCCC 
GGCCCCGATG ACCAGATGAT CATCGAGGCC ATCCAGGGCG TGCTCCGCGA GGTTGATTTG 
GACACGGTGA CGAAGAAGCA GGTGCGGGCG CTGGTGGAGC AGCGGTTGCA GACGGAGTTG 
GTGGGTGAGA GGAGGACGTT TATGGACAGG CAGATTGATA ATGAGTTGGC GAACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956331.3
CDS	654..6113
Translation	MANRMSMYSMASDAGMGGRGPGQQQPSQVSTTTLLNSVHNIYLSSQPYRLDAGTSLVVNTWLTASQAGPDGHVGGTVDGALALRAWEHARRRAEDGCIILGSLHNSTPSLLRPFLSTIPLEIPASVYKALDAIEPFLRCVTPYNPSTPRQTGLAVTLTLNLTGNLTAASLSLTQGGIDTDKGLLGVPSEPGYRAFDVFYYLLTTASTPAEREFLGLKAASQYSLLARSGTYDPPSYLPTADDGAAADDFRSALKDIGIKGSSHRNLISILAGLLKLGNTLDYNVDQEVLEEICEDVGGLLGIEPEILARQLSTDDRATLIGGLYEALVDWVIKKANDAIAVQMGKSRSGDDSSDGASRSGAPTSNEDSGDTVCLTVLEIPDQNLGKAIAMRGIFDDTQGINSEMKADGVEVVPVSSSVVREMQSAVAECGPDLGVMVGSVGRERQRLIEKREEILEKTAFASEDEGFLRKLLLPVPTEGINLGRTGRVDVADLLNRSRAWYHLCIHPTDDSPSSLAALPAVNSAWSAGAVSRQLRTWRLSEWANRRNKHIDYTADFDHHEFTQRYRPLGCMDGRDGIESWILERGWSNGEVVVGNERVWMRESAWWEAESMLDLKPMEAERLNSMRNLMAPGAMGSGYSHNGSGFFPPQVHADHMRSPFADGTSNGSYDHLALGGNLSQNNLNPSPARAPSVAPTNMTGMRSGDYGLGPKGDQYKGQVFYNAEGQLVSELEPDLAEGKKIEERPISSQRKIWVFTVWALTWWIPSPLLKFVGRMKRPDVRMAWREKFVLCLIIVLLNAIIVFWIIFFGRLLCPNFDKAWTLEEIQNHAAGDDFWVSIDGKVYDITKFWRLQHSDNNIDTTSDVMMPLAGQDLSAYFVAPLYITCPGLGIADSTTLQFNTTPEYVVAQHISGPTQPNIRSKLSERYWYTERFLPAIKEYYHGDVVWDSKKIKQEGLDWNHPWFIYEDLVYDMTDYMYTAKRMNNDATYTFIDAKITNMVKNNPGQDLTEQINDLIDRASSNTTQYNSVMNSLNCVKNAFYVGKPDFRYSARCQVNNYILLAFSVIICSIIVVKFVSALQFGSKRRPAPQDKFVICQVPAYTEGEDSLRKALDSLTALQYDNKRKLIFVICDGVIVGAGNDRPTPKIVLDILGVDPKVDPPALAFKSVGTGAEQLNYGKVYSGLYEFEGNVVPYIVVVKVGKESEQSKTKPGNRGKRDSQILLMSFLNRVHHRAPMNPLELEIFHQINNIIGVDPELYEYLLMVDADTCVREDSLNRLVAACANNAKIAGICGETSLQNEDLSWWTMIQVYEYYISHHLAKAFESLFGSVTCLPGCFTMYRLRTVDKGKPLIISDDIIREYSDCYVDTLHKKNLLSLGEDRFLTTLMTKHFPYMSYKFIPDAYCQTAAPETWSVLLSQRRRWINSTIHNLAELIFLDEMCGFCFFSMRIVVFIDLFGTIILPATCVYIVYLIYTASSHTAPFPLLSIIMIAAVYGLQALIFILKRQWQHVGWMIIYLLAFPIYSFILPIYSFWHQDDFAWGNTRVVIGESGKKQVVAVDEEGFNPRAIPLQRWDEYALANNLPGRRGGPLEKGMDEHLGGMYEETYEVDDMKSVYSSVRQPSVLTGFGGRGAGAYMPPHSPGFPPNGGGSGAMTHASTFVGASPFTEQNNLMNRQSMASMGTMVEMQRRASPYQDFPGGHSRGPSQGAMRSMTNTPPALGAGGMVNARLSTATGMSLGIPGHRPGMSHSESSRSFDFQRSTPGPDDQMIIEAIQGVLREVDLDTVTKKQVRALVEQRLQTELVGERRTFMDRQIDNELANM

Target ORF information:

RefSeq Version	XM_951238.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chitin synthase 6 (NCU04350), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_951238.3

ORF Insert Sequence:

ATGGCGAACC GCATGTCAAT GTATTCCATG GCCTCGGACG CCGGTATGGG CGGACGGGGT 
CCTGGTCAGC AACAGCCATC CCAGGTCTCT ACCACGACTC TTCTCAACTC CGTACACAAC 
ATCTACCTCT CTTCACAACC ATACCGTCTG GACGCCGGCA CAAGTCTCGT CGTCAACACA 
TGGCTTACGG CCTCCCAAGC TGGTCCCGAT GGCCACGTTG GTGGAACCGT TGATGGCGCT 
CTGGCTTTGA GGGCATGGGA GCACGCTCGT CGCCGTGCTG AGGATGGATG TATCATTCTT 
GGATCACTGC ACAACTCGAC TCCTTCGCTC TTGCGACCCT TCCTGTCCAC CATTCCCCTT 
GAGATCCCTG CCTCGGTGTA CAAAGCACTC GATGCTATTG AACCGTTCCT TCGCTGTGTA 
ACCCCTTACA ACCCCTCCAC TCCGAGACAG ACCGGCTTGG CCGTCACCTT GACCCTCAAC 
CTTACAGGCA ACCTTACTGC CGCCTCACTC TCGCTCACAC AAGGTGGTAT CGACACCGAC 
AAAGGCTTGT TGGGTGTCCC CTCTGAGCCT GGATACAGGG CCTTTGATGT CTTTTACTAC 
CTGCTCACCA CGGCCTCTAC CCCCGCCGAA AGAGAGTTCC TTGGGCTCAA GGCCGCGTCT 
CAATACAGTC TTCTCGCCCG GTCCGGCACC TACGATCCCC CATCCTATCT TCCTACCGCC 
GATGATGGTG CCGCTGCCGA CGACTTCAGG TCTGCCCTCA AGGACATCGG CATCAAGGGC 
TCTTCGCACC GCAACCTCAT CTCGATCTTG GCCGGCCTTC TCAAGCTCGG CAACACACTC 
GATTACAATG TTGACCAAGA AGTTTTGGAG GAAATCTGCG AGGATGTCGG TGGACTTCTT 
GGTATCGAAC CCGAAATTCT TGCCAGGCAA CTCAGCACCG ACGACCGGGC GACATTGATC 
GGAGGACTCT ATGAGGCTTT GGTCGATTGG GTCATCAAGA AGGCCAACGA TGCTATTGCC 
GTGCAGATGG GTAAATCCAG AAGTGGCGAT GATTCTAGCG ACGGTGCCTC CCGGAGTGGT 
GCGCCCACTT CCAACGAGGA CAGTGGCGAT ACTGTCTGCC TTACTGTCCT CGAGATTCCC 
GACCAAAACT TGGGCAAGGC TATTGCCATG CGGGGCATCT TCGACGACAC TCAGGGTATC 
AACTCGGAAA TGAAGGCTGA CGGCGTCGAG GTGGTTCCTG TTTCCAGCTC GGTTGTGCGG 
GAAATGCAGA GTGCTGTTGC AGAGTGTGGT CCTGATCTGG GTGTTATGGT TGGCTCGGTT 
GGCCGGGAAA GGCAACGCCT GATTGAGAAG CGCGAGGAAA TCTTGGAAAA GACTGCCTTC 
GCATCAGAAG ATGAGGGTTT CTTGAGGAAG CTCTTGCTCC CGGTTCCCAC CGAGGGTATC 
AACCTCGGCC GCACTGGCCG TGTGGATGTT GCGGATCTTT TGAACCGCAG TCGTGCTTGG 
TATCACCTCT GCATCCACCC CACAGACGAC TCACCTTCTA GTCTCGCTGC CCTTCCTGCC 
GTCAACTCAG CCTGGTCAGC TGGAGCTGTT TCTCGCCAGC TCCGCACCTG GAGGCTGTCC 
GAGTGGGCCA ACAGACGCAA TAAGCACATC GACTACACCG CCGATTTTGA TCACCATGAA 
TTTACTCAGC GATATCGGCC TCTGGGCTGT ATGGATGGCC GTGACGGTAT CGAGAGCTGG 
ATCCTGGAGC GGGGCTGGAG CAACGGCGAG GTTGTCGTGG GCAACGAGAG GGTATGGATG 
AGGGAGAGCG CGTGGTGGGA AGCCGAAAGT ATGTTGGACC TGAAGCCCAT GGAAGCTGAG 
AGGCTCAACA GCATGCGCAA CCTGATGGCC CCAGGGGCCA TGGGCTCGGG CTATTCTCAC 
AACGGAAGCG GCTTCTTCCC GCCTCAGGTA CATGCTGACC ACATGCGTTC TCCCTTTGCC 
GATGGAACCT CCAACGGGAG CTACGATCAC CTCGCCCTCG GTGGTAATCT TAGCCAAAAC 
AACCTCAACC CGAGCCCCGC GCGTGCTCCT TCCGTTGCCC CTACGAACAT GACTGGCATG 
CGTAGCGGTG ATTACGGTCT TGGTCCCAAG GGCGATCAAT ACAAGGGACA GGTCTTCTAC 
AACGCTGAAG GCCAACTGGT TTCGGAATTA GAGCCCGACC TTGCTGAGGG CAAGAAGATC 
GAGGAGCGGC CTATCTCCTC CCAAAGGAAA ATTTGGGTCT TTACTGTTTG GGCTTTGACG 
TGGTGGATTC CATCTCCATT ACTCAAGTTC GTCGGTCGGA TGAAGCGGCC TGACGTGCGT 
ATGGCATGGC GTGAGAAGTT TGTTCTCTGC CTCATTATCG TTCTTCTCAA CGCCATCATC 
GTTTTCTGGA TTATCTTCTT CGGTCGTCTT CTTTGCCCCA ATTTCGACAA AGCCTGGACT 
CTGGAAGAAA TTCAGAATCA TGCGGCAGGG GATGACTTCT GGGTCAGCAT TGACGGCAAG 
GTTTACGACA TCACCAAATT CTGGAGATTG CAGCACAGCG ACAACAACAT TGACACCACG 
TCGGATGTAA TGATGCCGCT TGCTGGCCAA GACCTCTCCG CTTACTTTGT CGCGCCTCTC 
TACATTACCT GCCCCGGCCT CGGCATCGCG GATTCAACTA CGTTGCAATT CAACACTACC 
CCTGAATATG TTGTGGCCCA GCATATCTCC GGCCCCACGC AGCCTAATAT TCGGAGCAAG 
CTCTCTGAAA GATACTGGTA TACCGAGAGA TTCTTGCCTG CCATCAAGGA GTACTACCAT 
GGCGACGTGG TTTGGGATTC CAAGAAGATT AAGCAGGAAG GCCTGGATTG GAATCACCCG 
TGGTTCATCT ACGAGGATCT CGTTTACGAC ATGACCGATT ACATGTACAC CGCCAAGCGT 
ATGAATAACG ATGCTACATA CACGTTCATC GACGCCAAGA TCACCAACAT GGTCAAGAAC 
AACCCCGGCC AGGACTTGAC CGAGCAGATT AACGACCTGA TTGATCGCGC CAGTAGCAAC 
ACGACCCAGT ATAATAGTGT TATGAACAGT CTTAACTGTG TCAAGAACGC GTTCTACGTT 
GGCAAGCCCG ATTTCCGTTA TTCGGCCAGG TGTCAAGTCA ACAACTACAT CTTGCTTGCA 
TTTTCCGTTA TTATCTGCAG TATCATCGTG GTCAAGTTCG TGTCGGCTCT TCAATTTGGA 
TCCAAGAGAC GCCCTGCTCC CCAGGACAAG TTTGTCATCT GCCAGGTGCC CGCTTATACC 
GAGGGTGAAG ACTCGCTGAG GAAAGCCCTG GACTCTTTGA CAGCCCTCCA GTACGATAAC 
AAGCGGAAGC TGATCTTTGT TATTTGCGAC GGTGTCATCG TCGGTGCTGG CAACGACCGT 
CCTACTCCCA AGATCGTCCT CGATATTCTC GGTGTTGACC CCAAGGTTGA CCCACCTGCC 
TTGGCCTTCA AATCCGTCGG TACTGGAGCC GAACAGCTCA ACTACGGCAA GGTCTACTCT 
GGATTGTATG AATTTGAAGG GAACGTGGTA CCTTATATCG TGGTTGTCAA GGTCGGCAAG 
GAGTCTGAGC AGTCCAAGAC GAAGCCCGGT AACCGTGGTA AGCGTGACTC GCAGATTCTG 
CTCATGAGCT TCCTCAACCG CGTCCATCAC CGTGCGCCCA TGAATCCCTT GGAACTGGAA 
ATCTTCCACC AGATCAACAA CATCATCGGT GTGGATCCCG AACTTTACGA ATACCTCCTC 
ATGGTCGATG CCGATACCTG CGTTCGCGAA GATTCTCTCA ACCGCCTAGT GGCAGCCTGC 
GCCAATAACG CCAAGATTGC CGGTATTTGC GGTGAAACCA GTTTGCAGAA TGAGGATCTT 
TCGTGGTGGA CCATGATCCA GGTTTACGAG TACTATATTT CTCATCACTT AGCGAAGGCT 
TTCGAGTCTC TCTTTGGCAG CGTTACCTGT TTGCCCGGTT GTTTCACCAT GTACCGCTTG 
CGTACTGTTG ACAAGGGAAA GCCGCTCATT ATCTCGGATG ATATTATCCG CGAGTACAGC 
GATTGCTATG TTGACACCCT CCACAAGAAG AACCTGCTCT CGCTTGGTGA GGATCGTTTC 
CTCACCACGC TCATGACCAA GCACTTCCCA TACATGTCTT ACAAGTTCAT TCCCGATGCC 
TACTGCCAAA CGGCCGCCCC CGAGACTTGG AGCGTTCTGC TGTCTCAGCG TCGTCGTTGG 
ATCAACTCAA CCATCCACAA CTTGGCTGAA TTGATCTTCC TCGACGAAAT GTGCGGTTTC 
TGTTTCTTCT CAATGCGCAT TGTCGTCTTC ATCGATCTCT TCGGTACTAT TATTCTTCCC 
GCCACTTGCG TCTACATCGT TTATCTCATT TACACGGCCT CCTCCCACAC TGCACCGTTC 
CCTCTTCTTT CGATCATCAT GATTGCGGCC GTGTACGGTC TCCAGGCGCT CATCTTCATC 
CTCAAGCGCC AATGGCAGCA CGTGGGCTGG ATGATCATCT ACCTCCTCGC CTTCCCCATT 
TACTCCTTCA TCCTGCCCAT CTACTCGTTC TGGCATCAGG ATGACTTCGC CTGGGGTAAT 
ACGCGCGTTG TCATTGGTGA AAGCGGTAAA AAGCAAGTTG TCGCCGTCGA CGAGGAGGGC 
TTCAACCCCC GTGCCATTCC TCTCCAGCGC TGGGACGAAT ATGCCCTCGC CAACAACCTT 
CCCGGTCGTC GCGGTGGCCC TCTTGAGAAG GGTATGGACG AGCATCTGGG CGGTATGTAC 
GAGGAGACCT ACGAGGTCGA TGACATGAAG TCAGTCTACT CTTCGGTCCG CCAACCGTCC 
GTTCTTACTG GGTTCGGCGG TCGCGGTGCC GGAGCTTACA TGCCCCCTCA CAGCCCTGGT 
TTCCCGCCCA ACGGCGGCGG TAGCGGCGCC ATGACCCATG CCAGCACCTT TGTCGGCGCC 
TCCCCTTTCA CCGAGCAGAA CAACCTCATG AACCGTCAGA GCATGGCCTC GATGGGCACC 
ATGGTCGAGA TGCAGCGCCG CGCCAGCCCG TACCAGGACT TCCCTGGCGG TCATAGCCGC 
GGCCCCAGCC AGGGTGCTAT GCGCTCCATG ACCAATACGC CTCCCGCTCT CGGCGCCGGC 
GGCATGGTCA ACGCCCGCCT GTCCACGGCC ACGGGCATGA GCCTGGGTAT TCCTGGACAC 
CGTCCCGGCA TGAGCCACTC GGAAAGTTCG CGGTCGTTTG ACTTTCAGCG CAGCACTCCC 
GGCCCCGATG ACCAGATGAT CATCGAGGCC ATCCAGGGCG TGCTCCGCGA GGTTGATTTG 
GACACGGTGA CGAAGAAGCA GGTGCGGGCG CTGGTGGAGC AGCGGTTGCA GACGGAGTTG 
GTGGGTGAGA GGAGGACGTT TATGGACAGG CAGATTGATA ATGAGTTGGC GAACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU04350 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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