NCU01823 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU01823
Entrez Gene ID	3872067
Full Name	two-component sensor protein histidine protein kinase
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_950827.2	XP_955920.2	two-component sensor protein histidine protein kinase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mak3	NP_588379.1
Schizosaccharomyces pombe (fission yeast)	mak2	NP_594410.1
Magnaporthe oryzae (rice blast fungus)	MGG_06696	XP_003709405.1
Neurospora crassa	NCU01823	XP_955920.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU01823 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU01823 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf09866	XM_950827.2 Latest version!	Neurospora crassa OR74A two-component sensor protein histidine protein kinase (NCU01823), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU01823 ( XM_950827.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_955920.2 Neurospora crassa OR74A two-component sensor protein histidine protein kinase (NCU01823), mRNA.

Price & Availability↑

CloneID	ONf09866
Clone ID Related Accession (Same CDS sequence)	XM_950827.2
Accession Version	XM_950827.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	two-component sensor protein histidine protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). On Feb 23, 2015 this sequence version replaced XM_950827.1.

ATGGAGTCAG TCGCGATACC TCCACAAGAC GACCAGCAGC AGCTCGATCC CCCGGCCCGC 
ATCTTTGAAC GATTACGCCA AATCGCCGGC TTTGCATGGG ACGAGACTAA ACCGCCTTAC 
CATTCTACCT ACGACAACTG GCACGTCTTC GGCACTCGAT TCGTTTCGCC CTTTTCCACC 
ACCGGAACCC CGGCCCCTGC CTCCCCGGCT CCATATCCTA ACACGAGTCC GTCGTCTTTA 
GCAAAGCTAG CACCCGGCCA CCGCGCAGCC CCCCAGGACC GCCCCAATGT TTCGGCCTCT 
CACAGCGACG CCGGCAGTGA TCGTTCCACC ATTTCTCTTC CCACCGTTGC CCACCATGCT 
ACCGATGCGC CTGTTGTCGA GGAGCTCGTC GTTGCCAGAA TATCCTACCA TGTCCTCCGC 
GAAGAGAGGG CCTACCATAT CACCAAAAGT CTTGTTTCGA CTGTTGATCC CAATGCCGAG 
CACATCTCGA GACCGATAGA CCTCATTCGT CTGAACCCCT TGCCAGGCGA TCGGGGGACC 
ATCGTTGTGG CCATCTACGC GTATTCTGGG GAAAATTATT TGTTCAAGGT GTTGGATATG 
GGCCCCGCCT TCTTCTACGC CAAAAAACAC AATGACCGAT GGGAGGCGCA CCGCGAAGAA 
CATCCGGTCC TCGAGGAGCC CATATCACTT CAGCACTTTC TGGATTTTGC CATAGGCGCT 
GCCCAATGTC TGGAGATGCT TCACCATAGC CAAAGCATTG TTCACGGCGA AATACGTCCC 
GATGCCTTCC ATTACAACCT CGAACTAAAC AGAATAAAGA TAACGTCACT CGGCTCTGGT 
CTACGGTCCT TCGAGCACGG TTTAACAAGC ACAGGCTGGT CCACACTGTC GAAGGAGGTG 
GGCGCCCGCC ATAAACTACA GTACATCAGC CCTGAACAAA CCGGACGGAT GCCTGCCGAG 
CCCGACACTC GCACCGATAT CTATTCCCTG GGGGTTTTGT TTTGGAGTCT CTTGACACAG 
CAGCCCGTGT TCCAGGGCGA CACACCGCTT GATATCGTCC AAGGTGTCCT TGGGCGTAGG 
ATACCTAACG TGTCCCAGAT ACGAATGGAT GTTCCCGACG CGATTGGCCG CATCATTCAA 
AAGTGCATTT CCAAAAACGT GAGCGATCGG TACTTTTCGG CCAGTGGCCT CCGGCACGAT 
TTGGTCAAGG TGCAAGAGTT GCTTGGCTCC GGCGATTCCC AGAGTCTCAG GGACTTTCAA 
GTTGGTTCCC GAGACGTATC TTCCTCCTTC CGGCTTCCGA CCATTATGAT TGGCCGCGAC 
CGCGAGCGAT CCGAGCTTGT CAAAGTCATT GAGCGAGTAT CCAAGAGCCA TGGGATGAAC 
CACAAAGGTG GGGTAAATTG GGGTTCAGAT GGATCTGTCT TGTCAAACGA TGTTGTTGAC 
AATGCTGAAG TCTCCAGTGA AGGCGCTAGC TCAGCCGACG GCTTGAACAG GCAGTCCGGC 
TCATGGGCCC TTGCTTTAAC CCCGGATCCG AGAAACAGAG TCAGCTTGCC CCCTTCGGCC 
CTGGGAACCA GTTCGGTGAC CACCTCGGGC GAAACGACGT CTTCAGGCAC GTCAACGGGT 
ACGTCGACGA TCTCGGGAGG AAATCGACAG TCTCGCCAGT GGGATAAGCC AGCTTTTGAA 
ACGTCCAGCT TGAACGAGAA TGTCAGCTCC GATGCCTCGC GGGGCAGTGG ATTGCTTAGC 
GAACCTACCT CGACGATATC ACGCCAGCTC GGTTCCAAGT TCCGAAAGCA GAGACAAGGC 
AATTGCGAGG TGGTGATCAT CGAGGGTACC GGCGGTCTAG GAAAGAGCTA TCTGGTCCAG 
TCAATACTGG CAGATGCGCG CAGGAGAGGT TATTGTGCTA CAGCCAAATT TGAAACAGCA 
AGGAGAACTG CCTTTGGCCC CCTTTTGCAG CTGCTATCCT CTCTCTTCCG TCAGGTCTGG 
GGCGAGAGAA ACACCGAGAC CCCTTTTCAT CAAGCGCTCA AACACCATGT CCGGCCTGTG 
TGGCCTACGT TGCATAAGGT CCTTGGCCTC CCCGAATTCC TGCTAGGCCC GCCCGACAAC 
ACCCCTCAAC GATCCAACTC CACTAGCTCA CAGAGCGGCC AGCCCGCGTT GAAACGACGC 
GGATCGTCTC CCGAGCTTGG CGGCAACAAC ATGTACCGAC ATTCTTCGAA AGTATCGTCC 
GCGACCCAGA CTTCACAGGA GTTTCTGAGG GCTGGGGCTT CGACGCAGAC TTCCCGCCTA 
ACGAACACCT TCCTCGATGT ACTGCGGATG TTCACACACT ACAAATTCAT TTGCTTCTGT 
CTCGATGATC TGCACTTTGC GGACGACGAG TCACAGGAGC TCATTAGCCA GATCATCGGA 
GCGCGAATGA AAATGGTCAT CATCATCACG TACCGCCCGG AAGAGATCGG GCCCGAGAAA 
GTCCTGCAAA TCCTCAGTCC ACCAAAGTCC GAAGAGCTAC CACGTGGAGC AGGCGCACCA 
ATAATGACCA AAATAAAGCT AACGCCTCTG TCCGAGGACG ACATAATACA CTTCGTATCG 
TCGACACTCT GTCTACCGAA GGAAGATGTC ACGCCTTTGG CTCTTGTCAT CCAATCCAAG 
ACAGCTGGAA ACCCCTTTTA TATGCGTGAG ATGCTCAATG CTGGCTATAG GAAGAAGTGC 
ATCTGGTATG ACTACATGGC TGGTGAATGG AAGTACGACT TGGACAAGCT CTTCGACCAG 
TTTCAAGGGG AACAGAACTA CGATGTTCTC GACACCGCAT TTATCACCCG ACGCTTAAGT 
GAGCTGCCGC CCGCCTCACG GTCAATCCTT GCTTGGGCTG CGTTACTCGG GCAGACTTTT 
TCGTTTGACC TTATCTGCCG ACTCATGACT GGCGAGTTTC ATTTTGAAGA AGACAAGGAC 
AAGCGACAGC AAGAAGAAGA TTGCTCATAT ATCATACCCG CAGATGAGTC CGTTGCTGGA 
CTTGAAGCAG CCATACAAGC CTGCATCATT GTTCCAAGCG ATAGGGATGA TCATTTTCGT 
TTCGTCCACG ATCGCTACAT CCAGGCGGCT GCGGCATTAC AAGAATGCAA TGCTCGAAAA 
ATGCACTTTG CCATCGCCCA AACACTCCTC AAATACCACG CCAACGATCG TACTTACAGG 
GACAATGCGG CCTCCCACAT TTGCGAGTGC GTCGATATCA TCTGCCAGCA AGTGTCTCGT 
CGGCGTTCGT ATCGAGCATT GCTCTCCCGG TGTGCACAAG AAGCATCCGT GAGTGGCGCA 
AGACCTACTG CAGCAAAATT CTACGGCAAC GCTGTAGCGC TACTTCAACC TGACCCTTGG 
AACGACGATG CGGGCGATTC CTCGTATGAA GAGACTCTCC AACTCTATAT CCAAGCAGCC 
GAATGCTACC TTTACATGGG CCTCCACGCC GCCGCCAACG ATCTACTCCA GACAATTCTC 
GCCAAGGCAA GGACGGCTGT TGACAAGGCC CCGGCCTGGG TACTGCAGTC ACGAATTTTT 
GCTCAGCGCG GAAACTCCGA ACAGGCATTG GACTCACTCC GGTTGTGTTT GAAAGCTTTG 
GGTGTAGGGT TCGATGACAA CCCATCCTTT GAGAAGTGCG ACGCCCAATT CGAGCGCCTG 
TCCGTACGAA TACAGACAAT GGATCGCAGC GAGCTGGTCA ACCCGCCCAA GACGGAAGAT 
CTCACTCTTG CATCCCTTGG TGCCGTGCTC ACCGAGACCG TTAGTGCTGG ATGGTGGAGC 
AACTGTCTGT ACTTTTACCA ACTTGCGCTC TCTATGATGG AGTTACACTT GGAGAAAGGT 
TCATTCCCCC AATCTGGCAT GGCATTTCTA TTCTTGGGCA CCATCGCCCT CTCGAGGTTC 
AACATGGTTC AATTCACTGT CGACTTGGCC GGCATTTTCC TGGAGCTGCT GGAGAAGTAC 
CGCGATCCAT ATTCGATGGC CAGGGGCTAC ATGATCTATG CCAACTTTGT CGGTCATGTA 
CAGTTTCCGC TCGGCGCTAT TGCCAACCAG CTTGAGGCAT CGGTCGAGTT CGCCGCTGCT 
ATGGGAGACC GCATCTCTGC CATTCTTAGC TTTGGTCTAG CTGCACAGCT GAAATTTTTT 
GCCAGCGAAA ATATCTCCGA TCTCGAGGCC TTCTGCCAGT ATGGCTGTGA GGAGGTACCG 
AATTGGCATC TGGACACTCG TGGCGGCGTA ATTCTTATTG CCATTCGACA GGTGTGCCGT 
GCCTTGCAAG GGAAAACGCA AGTGACAGAT CCTTATGAGG TCATGAGTGA CGAACAACAC 
AAAGCCTCAA CTTACAAGTC TTGGCTCACA GCCAAGGCCA GTGGAGACAG GACGCTTATG 
TTCTACGAGT CAATCGAGAT TATCCCGCTC TTCCTGTATG GGCACTATGA GCGGGCCATT 
GAGATCGGGG AGGTTTGTTG CAAGAACAGC AAGATGATTT GGTCGGCCAG GAACACAAAG 
CTCGCACAGT GGTTTTACGC TTTGTCTTTG GCGGCTGTGA TCCTCCGCGA ACAGCAAGAC 
CCTCGGCGCA TGGCTGAAGA TGAGGAGAAG TTTCAGAGCC GCGTGGAGGA GACGATTGAG 
AAGATTGATG AACTGAACAA GGGGCTGAAG GACTGGCAGG CAGTTAATAA TGTTAACTAC 
CTGGCTTGGT CAAAGTTACT GGATGCCCAA ATAGCAGAGA TCCGTGGTCA ATATGGGGAT 
GCCATTCAGC AATATGAAGA GGCGTTGGAT CATGCTGCAG AATACAATTT CCTCTTCGAG 
GAGGCCTTGG GAAATTACCT GATGGCTAGC GTCTTCATTC GTCACAAGGG CAGGCGCTCT 
GCCCGGTCTA CCCTTCGCGA CTCTATTGCT CTCTACCGCC AGCTTGGTGC CACAGGAATC 
GCCGACCTCA TCGAGACCGG TCATAGTATT TTGCTCCATG GCCCTATGCG CAACCCTCGC 
ACGGCTGATG CTGGCGTTCA GACCGATTTT ACTGGCGACG CTGCGTCTGT TCAATATCGG 
ACCGTGGAAG AAGAAGGATT GGAGGACGGA GAGGAGACGG CGCAGGCTGC GGTGGCAGCG 
CTGAAAGGGG AACGCATGAC AGCGTGGAGG GGCTCAATGC AGCCGGAGGC TGGGGTTGGC 
TTGCCAGCAC TTGATATGAT CGATCTGCAC GCGATTTTGG TCTCATCTCA GGTCATCTCC 
TCGGTGTTGA GGGTTGATGA GCTTCTCAAG ACCATGTGCG ACGTAATCTT GCAGACGTGC 
AGCGGGTCCG CTACCCTGGC AGCTCTCGTG GTGTATGAGG ATGAGAAGCA GAAGGACAAT 
TCGCCCCTGT GTGTTGCTGC GAGTGGTGAC CCCGATAAAG GAGCTGAGGC GCACATTCCC 
GGCATTCCCC TCAGCGGAAC CGACCTCGTC GCCGAGAATG TCATCCTGTA CTGCTCCCGC 
TTCCGTGAGG TGGTTTTTCT TCGGGACCTT GTAAGCGATG AGCGGTTCGG CAACGTTAGC 
GAAAGTTGGC TTCGGAGGAA CCCAAACAGC AAAGCGGTCA TTGCCATCCC GATCTTGCAT 
GGTTCTGAAA ACCCGCTACT TGGCGTCTTG TACCTCGAAG GCGTTCCCGG ATCATTCACA 
GACCGAAACG TCACTGTTCT GCAGCTTCTC GTCAACCAGA TCGGAATCAG TTACTCGAAC 
GCTCTGGCCA TGAAAGCTGT TGAAAAGGTT TCGGCTGAGA ACGTCTCCAT GGTGGCGCTC 
CAGAAGCGTG CTTTGGCTAA AGCGGTTGAA GCAGAAACCA AAGCCAGAAA TGCCGAAGCT 
GAAGCCATCC GGAACGTGAA GCTTGCGGAA GAAGCCGCGA AGGCGAAGTC AATCTTCCTC 
GCTAATGTCT CTCACGAGCT GCGCACGCCC CTAAATGGAG TGATTGGAAA CTCGGAGCTG 
CTCAGGGATA GCAACTTGAA TAAGGAGCAA CTGGAAATGG CTGACTCGAT ACGTGTGTCG 
GCAGATCTGC TACTTACAGT CATCAACGAC ATCCTGGACT TTTCAAGGAT GGAGGCAGAC 
AAGATGAAGC TCTACATCAT CGCATTCAAC CCGGAGGAGA TGGTTCGCGA AGTCGTCCGC 
GCAGCATCGT ACAGCAACAA GGAAAAGACA TCCAAGAAGA ACGTCAAGAT CGTTCACGAC 
ATCAATCTTC CTCCCATGTT GATTTACGGC GATCCCATTC GTCTTCACCA GGTCCTGGGC 
AATCTCATTG GCAACAGCCT GAAGTTCACA GAGCATGGCT CCATCACCAT TGGGGCCAAG 
GTCGACTCCG AAACCACAGA GAAGGCGACG TTGACATTTT GGGTCAAGGA TACCGGCATC 
GGCATTTCAC CACAGCAACT GGACAAGCTC TTCCTTCCCT TTAGCCAAGC CGACGAGAGC 
ACAGCGCGGA AATATGGCGG CAGTGGTCTC GGTCTTAGTA TCTGCAAGTC ACTGATCGAA 
TCGACCATGA AGGGCAAGAT CCGCTTGGAC AGTGAGGAGA ACGTGGGGAC AACCGTCTGG 
TTCACTGTCA CGTTTGATAA GGCCAAGCCG GAGGTGATGG CAGGTGATAC CCAGAGCACA 
TCGCCCGTTC AGATGGAGCC TCCACGGGAC CGCCAACCGC GGCCACTTCC GGGCGCTGTG 
GAAAACGGCG ATATCAGCAG TGATCAGGTG CCGCGGCCCT ACATCGACCT TACCCATATC 
CCACGCAACC AGCTTCGAAT ATGCATTGCC GAAGACAATT CCATCAATGC TAAGATTGCC 
ATGCAGTATA TGCACAAGCT GGGTTATCCG AATGTTGATA CCTATGACAA CGGCCTGAAA 
GCAGTTGAGG GTCTGCGGGA GAAGGCGCGG CAGGGAAACC CGTATCACAT CATACTAATG 
GATGTACAAA TGCCAGTGCT GGATGGTTAT GAGGCGACGA AGATGTTGCG AAACGATCCG 
ATAGATTCTG TCCGCAATGT TCTCGTAATA GCCATGACAG CCTCGGCCAT TCAGGGCGAT 
CGAGAGAAGT GTCTCGCTGC GGGTATGAAC GATTATCTTG CCAAGCCGGT TCGAGGGGAG 
CTGCTGAAGA GAAAGCTGGA TACTTATCTG GGAGCGAGCG CGAGCATGAG TTTCAGCACT 
CAGCGTTTTC TCGAACGGCG CTTCGCCCAA AGGCCTGGAA GCCCCCAACC CATCAACCCC 
GGCAGCAGCA GTACCAAAAA AGACGTTGAC ACTCCACTTT CCTCGCCCGT GCCACCTCCT 
TTGGCTGATA ACACCGCTGC TTCTGCTTCC ACTGTCAAAA TCGGAGTGGG ATCCGCTGCG 
GCTTCTCTTC GAGAGGTGGC GGCCAGCAAC GTGAGCGGCG GTGAATCGAG ACCGTCTTCA 
CCCAAACTCC TCTCTCCCAG CGGCAAGAAC AGCAAACAAA GCCACAGAAG AGGCAGATCA 
TACGATATCC GCCTCCTTCG CCCCTCGGAG TCCGCAACAA GCTTCAGTAA CGTCACAAAT 
ACCAGCGGCG GAAGCAGCAG CAACAGCAAC AGCAGCAGCA AGAAGTTGGA CAGGCGTTTG 
TCCTCTCCAT CTTTGTCCTC CAGTGAGCGG CGAGGATCGG TGCCGGACCT GACAGCTGGT 
GGCGGCGGTG GTGATAAGCG GCAGCCTAAG AAGCTGGTGG AGGCTCGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_955920.2
CDS	855..8528
Translation	MESVAIPPQDDQQQLDPPARIFERLRQIAGFAWDETKPPYHSTYDNWHVFGTRFVSPFSTTGTPAPASPAPYPNTSPSSLAKLAPGHRAAPQDRPNVSASHSDAGSDRSTISLPTVAHHATDAPVVEELVVARISYHVLREERAYHITKSLVSTVDPNAEHISRPIDLIRLNPLPGDRGTIVVAIYAYSGENYLFKVLDMGPAFFYAKKHNDRWEAHREEHPVLEEPISLQHFLDFAIGAAQCLEMLHHSQSIVHGEIRPDAFHYNLELNRIKITSLGSGLRSFEHGLTSTGWSTLSKEVGARHKLQYISPEQTGRMPAEPDTRTDIYSLGVLFWSLLTQQPVFQGDTPLDIVQGVLGRRIPNVSQIRMDVPDAIGRIIQKCISKNVSDRYFSASGLRHDLVKVQELLGSGDSQSLRDFQVGSRDVSSSFRLPTIMIGRDRERSELVKVIERVSKSHGMNHKGGVNWGSDGSVLSNDVVDNAEVSSEGASSADGLNRQSGSWALALTPDPRNRVSLPPSALGTSSVTTSGETTSSGTSTGTSTISGGNRQSRQWDKPAFETSSLNENVSSDASRGSGLLSEPTSTISRQLGSKFRKQRQGNCEVVIIEGTGGLGKSYLVQSILADARRRGYCATAKFETARRTAFGPLLQLLSSLFRQVWGERNTETPFHQALKHHVRPVWPTLHKVLGLPEFLLGPPDNTPQRSNSTSSQSGQPALKRRGSSPELGGNNMYRHSSKVSSATQTSQEFLRAGASTQTSRLTNTFLDVLRMFTHYKFICFCLDDLHFADDESQELISQIIGARMKMVIIITYRPEEIGPEKVLQILSPPKSEELPRGAGAPIMTKIKLTPLSEDDIIHFVSSTLCLPKEDVTPLALVIQSKTAGNPFYMREMLNAGYRKKCIWYDYMAGEWKYDLDKLFDQFQGEQNYDVLDTAFITRRLSELPPASRSILAWAALLGQTFSFDLICRLMTGEFHFEEDKDKRQQEEDCSYIIPADESVAGLEAAIQACIIVPSDRDDHFRFVHDRYIQAAAALQECNARKMHFAIAQTLLKYHANDRTYRDNAASHICECVDIICQQVSRRRSYRALLSRCAQEASVSGARPTAAKFYGNAVALLQPDPWNDDAGDSSYEETLQLYIQAAECYLYMGLHAAANDLLQTILAKARTAVDKAPAWVLQSRIFAQRGNSEQALDSLRLCLKALGVGFDDNPSFEKCDAQFERLSVRIQTMDRSELVNPPKTEDLTLASLGAVLTETVSAGWWSNCLYFYQLALSMMELHLEKGSFPQSGMAFLFLGTIALSRFNMVQFTVDLAGIFLELLEKYRDPYSMARGYMIYANFVGHVQFPLGAIANQLEASVEFAAAMGDRISAILSFGLAAQLKFFASENISDLEAFCQYGCEEVPNWHLDTRGGVILIAIRQVCRALQGKTQVTDPYEVMSDEQHKASTYKSWLTAKASGDRTLMFYESIEIIPLFLYGHYERAIEIGEVCCKNSKMIWSARNTKLAQWFYALSLAAVILREQQDPRRMAEDEEKFQSRVEETIEKIDELNKGLKDWQAVNNVNYLAWSKLLDAQIAEIRGQYGDAIQQYEEALDHAAEYNFLFEEALGNYLMASVFIRHKGRRSARSTLRDSIALYRQLGATGIADLIETGHSILLHGPMRNPRTADAGVQTDFTGDAASVQYRTVEEEGLEDGEETAQAAVAALKGERMTAWRGSMQPEAGVGLPALDMIDLHAILVSSQVISSVLRVDELLKTMCDVILQTCSGSATLAALVVYEDEKQKDNSPLCVAASGDPDKGAEAHIPGIPLSGTDLVAENVILYCSRFREVVFLRDLVSDERFGNVSESWLRRNPNSKAVIAIPILHGSENPLLGVLYLEGVPGSFTDRNVTVLQLLVNQIGISYSNALAMKAVEKVSAENVSMVALQKRALAKAVEAETKARNAEAEAIRNVKLAEEAAKAKSIFLANVSHELRTPLNGVIGNSELLRDSNLNKEQLEMADSIRVSADLLLTVINDILDFSRMEADKMKLYIIAFNPEEMVREVVRAASYSNKEKTSKKNVKIVHDINLPPMLIYGDPIRLHQVLGNLIGNSLKFTEHGSITIGAKVDSETTEKATLTFWVKDTGIGISPQQLDKLFLPFSQADESTARKYGGSGLGLSICKSLIESTMKGKIRLDSEENVGTTVWFTVTFDKAKPEVMAGDTQSTSPVQMEPPRDRQPRPLPGAVENGDISSDQVPRPYIDLTHIPRNQLRICIAEDNSINAKIAMQYMHKLGYPNVDTYDNGLKAVEGLREKARQGNPYHIILMDVQMPVLDGYEATKMLRNDPIDSVRNVLVIAMTASAIQGDREKCLAAGMNDYLAKPVRGELLKRKLDTYLGASASMSFSTQRFLERRFAQRPGSPQPINPGSSSTKKDVDTPLSSPVPPPLADNTAASASTVKIGVGSAAASLREVAASNVSGGESRPSSPKLLSPSGKNSKQSHRRGRSYDIRLLRPSESATSFSNVTNTSGGSSSNSNSSSKKLDRRLSSPSLSSSERRGSVPDLTAGGGGGDKRQPKKLVEARE

Target ORF information:

RefSeq Version	XM_950827.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A two-component sensor protein histidine protein kinase (NCU01823), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_950827.2

ORF Insert Sequence:

ATGGAGTCAG TCGCGATACC TCCACAAGAC GACCAGCAGC AGCTCGATCC CCCGGCCCGC 
ATCTTTGAAC GATTACGCCA AATCGCCGGC TTTGCATGGG ACGAGACTAA ACCGCCTTAC 
CATTCTACCT ACGACAACTG GCACGTCTTC GGCACTCGAT TCGTTTCGCC CTTTTCCACC 
ACCGGAACCC CGGCCCCTGC CTCCCCGGCT CCATATCCTA ACACGAGTCC GTCGTCTTTA 
GCAAAGCTAG CACCCGGCCA CCGCGCAGCC CCCCAGGACC GCCCCAATGT TTCGGCCTCT 
CACAGCGACG CCGGCAGTGA TCGTTCCACC ATTTCTCTTC CCACCGTTGC CCACCATGCT 
ACCGATGCGC CTGTTGTCGA GGAGCTCGTC GTTGCCAGAA TATCCTACCA TGTCCTCCGC 
GAAGAGAGGG CCTACCATAT CACCAAAAGT CTTGTTTCGA CTGTTGATCC CAATGCCGAG 
CACATCTCGA GACCGATAGA CCTCATTCGT CTGAACCCCT TGCCAGGCGA TCGGGGGACC 
ATCGTTGTGG CCATCTACGC GTATTCTGGG GAAAATTATT TGTTCAAGGT GTTGGATATG 
GGCCCCGCCT TCTTCTACGC CAAAAAACAC AATGACCGAT GGGAGGCGCA CCGCGAAGAA 
CATCCGGTCC TCGAGGAGCC CATATCACTT CAGCACTTTC TGGATTTTGC CATAGGCGCT 
GCCCAATGTC TGGAGATGCT TCACCATAGC CAAAGCATTG TTCACGGCGA AATACGTCCC 
GATGCCTTCC ATTACAACCT CGAACTAAAC AGAATAAAGA TAACGTCACT CGGCTCTGGT 
CTACGGTCCT TCGAGCACGG TTTAACAAGC ACAGGCTGGT CCACACTGTC GAAGGAGGTG 
GGCGCCCGCC ATAAACTACA GTACATCAGC CCTGAACAAA CCGGACGGAT GCCTGCCGAG 
CCCGACACTC GCACCGATAT CTATTCCCTG GGGGTTTTGT TTTGGAGTCT CTTGACACAG 
CAGCCCGTGT TCCAGGGCGA CACACCGCTT GATATCGTCC AAGGTGTCCT TGGGCGTAGG 
ATACCTAACG TGTCCCAGAT ACGAATGGAT GTTCCCGACG CGATTGGCCG CATCATTCAA 
AAGTGCATTT CCAAAAACGT GAGCGATCGG TACTTTTCGG CCAGTGGCCT CCGGCACGAT 
TTGGTCAAGG TGCAAGAGTT GCTTGGCTCC GGCGATTCCC AGAGTCTCAG GGACTTTCAA 
GTTGGTTCCC GAGACGTATC TTCCTCCTTC CGGCTTCCGA CCATTATGAT TGGCCGCGAC 
CGCGAGCGAT CCGAGCTTGT CAAAGTCATT GAGCGAGTAT CCAAGAGCCA TGGGATGAAC 
CACAAAGGTG GGGTAAATTG GGGTTCAGAT GGATCTGTCT TGTCAAACGA TGTTGTTGAC 
AATGCTGAAG TCTCCAGTGA AGGCGCTAGC TCAGCCGACG GCTTGAACAG GCAGTCCGGC 
TCATGGGCCC TTGCTTTAAC CCCGGATCCG AGAAACAGAG TCAGCTTGCC CCCTTCGGCC 
CTGGGAACCA GTTCGGTGAC CACCTCGGGC GAAACGACGT CTTCAGGCAC GTCAACGGGT 
ACGTCGACGA TCTCGGGAGG AAATCGACAG TCTCGCCAGT GGGATAAGCC AGCTTTTGAA 
ACGTCCAGCT TGAACGAGAA TGTCAGCTCC GATGCCTCGC GGGGCAGTGG ATTGCTTAGC 
GAACCTACCT CGACGATATC ACGCCAGCTC GGTTCCAAGT TCCGAAAGCA GAGACAAGGC 
AATTGCGAGG TGGTGATCAT CGAGGGTACC GGCGGTCTAG GAAAGAGCTA TCTGGTCCAG 
TCAATACTGG CAGATGCGCG CAGGAGAGGT TATTGTGCTA CAGCCAAATT TGAAACAGCA 
AGGAGAACTG CCTTTGGCCC CCTTTTGCAG CTGCTATCCT CTCTCTTCCG TCAGGTCTGG 
GGCGAGAGAA ACACCGAGAC CCCTTTTCAT CAAGCGCTCA AACACCATGT CCGGCCTGTG 
TGGCCTACGT TGCATAAGGT CCTTGGCCTC CCCGAATTCC TGCTAGGCCC GCCCGACAAC 
ACCCCTCAAC GATCCAACTC CACTAGCTCA CAGAGCGGCC AGCCCGCGTT GAAACGACGC 
GGATCGTCTC CCGAGCTTGG CGGCAACAAC ATGTACCGAC ATTCTTCGAA AGTATCGTCC 
GCGACCCAGA CTTCACAGGA GTTTCTGAGG GCTGGGGCTT CGACGCAGAC TTCCCGCCTA 
ACGAACACCT TCCTCGATGT ACTGCGGATG TTCACACACT ACAAATTCAT TTGCTTCTGT 
CTCGATGATC TGCACTTTGC GGACGACGAG TCACAGGAGC TCATTAGCCA GATCATCGGA 
GCGCGAATGA AAATGGTCAT CATCATCACG TACCGCCCGG AAGAGATCGG GCCCGAGAAA 
GTCCTGCAAA TCCTCAGTCC ACCAAAGTCC GAAGAGCTAC CACGTGGAGC AGGCGCACCA 
ATAATGACCA AAATAAAGCT AACGCCTCTG TCCGAGGACG ACATAATACA CTTCGTATCG 
TCGACACTCT GTCTACCGAA GGAAGATGTC ACGCCTTTGG CTCTTGTCAT CCAATCCAAG 
ACAGCTGGAA ACCCCTTTTA TATGCGTGAG ATGCTCAATG CTGGCTATAG GAAGAAGTGC 
ATCTGGTATG ACTACATGGC TGGTGAATGG AAGTACGACT TGGACAAGCT CTTCGACCAG 
TTTCAAGGGG AACAGAACTA CGATGTTCTC GACACCGCAT TTATCACCCG ACGCTTAAGT 
GAGCTGCCGC CCGCCTCACG GTCAATCCTT GCTTGGGCTG CGTTACTCGG GCAGACTTTT 
TCGTTTGACC TTATCTGCCG ACTCATGACT GGCGAGTTTC ATTTTGAAGA AGACAAGGAC 
AAGCGACAGC AAGAAGAAGA TTGCTCATAT ATCATACCCG CAGATGAGTC CGTTGCTGGA 
CTTGAAGCAG CCATACAAGC CTGCATCATT GTTCCAAGCG ATAGGGATGA TCATTTTCGT 
TTCGTCCACG ATCGCTACAT CCAGGCGGCT GCGGCATTAC AAGAATGCAA TGCTCGAAAA 
ATGCACTTTG CCATCGCCCA AACACTCCTC AAATACCACG CCAACGATCG TACTTACAGG 
GACAATGCGG CCTCCCACAT TTGCGAGTGC GTCGATATCA TCTGCCAGCA AGTGTCTCGT 
CGGCGTTCGT ATCGAGCATT GCTCTCCCGG TGTGCACAAG AAGCATCCGT GAGTGGCGCA 
AGACCTACTG CAGCAAAATT CTACGGCAAC GCTGTAGCGC TACTTCAACC TGACCCTTGG 
AACGACGATG CGGGCGATTC CTCGTATGAA GAGACTCTCC AACTCTATAT CCAAGCAGCC 
GAATGCTACC TTTACATGGG CCTCCACGCC GCCGCCAACG ATCTACTCCA GACAATTCTC 
GCCAAGGCAA GGACGGCTGT TGACAAGGCC CCGGCCTGGG TACTGCAGTC ACGAATTTTT 
GCTCAGCGCG GAAACTCCGA ACAGGCATTG GACTCACTCC GGTTGTGTTT GAAAGCTTTG 
GGTGTAGGGT TCGATGACAA CCCATCCTTT GAGAAGTGCG ACGCCCAATT CGAGCGCCTG 
TCCGTACGAA TACAGACAAT GGATCGCAGC GAGCTGGTCA ACCCGCCCAA GACGGAAGAT 
CTCACTCTTG CATCCCTTGG TGCCGTGCTC ACCGAGACCG TTAGTGCTGG ATGGTGGAGC 
AACTGTCTGT ACTTTTACCA ACTTGCGCTC TCTATGATGG AGTTACACTT GGAGAAAGGT 
TCATTCCCCC AATCTGGCAT GGCATTTCTA TTCTTGGGCA CCATCGCCCT CTCGAGGTTC 
AACATGGTTC AATTCACTGT CGACTTGGCC GGCATTTTCC TGGAGCTGCT GGAGAAGTAC 
CGCGATCCAT ATTCGATGGC CAGGGGCTAC ATGATCTATG CCAACTTTGT CGGTCATGTA 
CAGTTTCCGC TCGGCGCTAT TGCCAACCAG CTTGAGGCAT CGGTCGAGTT CGCCGCTGCT 
ATGGGAGACC GCATCTCTGC CATTCTTAGC TTTGGTCTAG CTGCACAGCT GAAATTTTTT 
GCCAGCGAAA ATATCTCCGA TCTCGAGGCC TTCTGCCAGT ATGGCTGTGA GGAGGTACCG 
AATTGGCATC TGGACACTCG TGGCGGCGTA ATTCTTATTG CCATTCGACA GGTGTGCCGT 
GCCTTGCAAG GGAAAACGCA AGTGACAGAT CCTTATGAGG TCATGAGTGA CGAACAACAC 
AAAGCCTCAA CTTACAAGTC TTGGCTCACA GCCAAGGCCA GTGGAGACAG GACGCTTATG 
TTCTACGAGT CAATCGAGAT TATCCCGCTC TTCCTGTATG GGCACTATGA GCGGGCCATT 
GAGATCGGGG AGGTTTGTTG CAAGAACAGC AAGATGATTT GGTCGGCCAG GAACACAAAG 
CTCGCACAGT GGTTTTACGC TTTGTCTTTG GCGGCTGTGA TCCTCCGCGA ACAGCAAGAC 
CCTCGGCGCA TGGCTGAAGA TGAGGAGAAG TTTCAGAGCC GCGTGGAGGA GACGATTGAG 
AAGATTGATG AACTGAACAA GGGGCTGAAG GACTGGCAGG CAGTTAATAA TGTTAACTAC 
CTGGCTTGGT CAAAGTTACT GGATGCCCAA ATAGCAGAGA TCCGTGGTCA ATATGGGGAT 
GCCATTCAGC AATATGAAGA GGCGTTGGAT CATGCTGCAG AATACAATTT CCTCTTCGAG 
GAGGCCTTGG GAAATTACCT GATGGCTAGC GTCTTCATTC GTCACAAGGG CAGGCGCTCT 
GCCCGGTCTA CCCTTCGCGA CTCTATTGCT CTCTACCGCC AGCTTGGTGC CACAGGAATC 
GCCGACCTCA TCGAGACCGG TCATAGTATT TTGCTCCATG GCCCTATGCG CAACCCTCGC 
ACGGCTGATG CTGGCGTTCA GACCGATTTT ACTGGCGACG CTGCGTCTGT TCAATATCGG 
ACCGTGGAAG AAGAAGGATT GGAGGACGGA GAGGAGACGG CGCAGGCTGC GGTGGCAGCG 
CTGAAAGGGG AACGCATGAC AGCGTGGAGG GGCTCAATGC AGCCGGAGGC TGGGGTTGGC 
TTGCCAGCAC TTGATATGAT CGATCTGCAC GCGATTTTGG TCTCATCTCA GGTCATCTCC 
TCGGTGTTGA GGGTTGATGA GCTTCTCAAG ACCATGTGCG ACGTAATCTT GCAGACGTGC 
AGCGGGTCCG CTACCCTGGC AGCTCTCGTG GTGTATGAGG ATGAGAAGCA GAAGGACAAT 
TCGCCCCTGT GTGTTGCTGC GAGTGGTGAC CCCGATAAAG GAGCTGAGGC GCACATTCCC 
GGCATTCCCC TCAGCGGAAC CGACCTCGTC GCCGAGAATG TCATCCTGTA CTGCTCCCGC 
TTCCGTGAGG TGGTTTTTCT TCGGGACCTT GTAAGCGATG AGCGGTTCGG CAACGTTAGC 
GAAAGTTGGC TTCGGAGGAA CCCAAACAGC AAAGCGGTCA TTGCCATCCC GATCTTGCAT 
GGTTCTGAAA ACCCGCTACT TGGCGTCTTG TACCTCGAAG GCGTTCCCGG ATCATTCACA 
GACCGAAACG TCACTGTTCT GCAGCTTCTC GTCAACCAGA TCGGAATCAG TTACTCGAAC 
GCTCTGGCCA TGAAAGCTGT TGAAAAGGTT TCGGCTGAGA ACGTCTCCAT GGTGGCGCTC 
CAGAAGCGTG CTTTGGCTAA AGCGGTTGAA GCAGAAACCA AAGCCAGAAA TGCCGAAGCT 
GAAGCCATCC GGAACGTGAA GCTTGCGGAA GAAGCCGCGA AGGCGAAGTC AATCTTCCTC 
GCTAATGTCT CTCACGAGCT GCGCACGCCC CTAAATGGAG TGATTGGAAA CTCGGAGCTG 
CTCAGGGATA GCAACTTGAA TAAGGAGCAA CTGGAAATGG CTGACTCGAT ACGTGTGTCG 
GCAGATCTGC TACTTACAGT CATCAACGAC ATCCTGGACT TTTCAAGGAT GGAGGCAGAC 
AAGATGAAGC TCTACATCAT CGCATTCAAC CCGGAGGAGA TGGTTCGCGA AGTCGTCCGC 
GCAGCATCGT ACAGCAACAA GGAAAAGACA TCCAAGAAGA ACGTCAAGAT CGTTCACGAC 
ATCAATCTTC CTCCCATGTT GATTTACGGC GATCCCATTC GTCTTCACCA GGTCCTGGGC 
AATCTCATTG GCAACAGCCT GAAGTTCACA GAGCATGGCT CCATCACCAT TGGGGCCAAG 
GTCGACTCCG AAACCACAGA GAAGGCGACG TTGACATTTT GGGTCAAGGA TACCGGCATC 
GGCATTTCAC CACAGCAACT GGACAAGCTC TTCCTTCCCT TTAGCCAAGC CGACGAGAGC 
ACAGCGCGGA AATATGGCGG CAGTGGTCTC GGTCTTAGTA TCTGCAAGTC ACTGATCGAA 
TCGACCATGA AGGGCAAGAT CCGCTTGGAC AGTGAGGAGA ACGTGGGGAC AACCGTCTGG 
TTCACTGTCA CGTTTGATAA GGCCAAGCCG GAGGTGATGG CAGGTGATAC CCAGAGCACA 
TCGCCCGTTC AGATGGAGCC TCCACGGGAC CGCCAACCGC GGCCACTTCC GGGCGCTGTG 
GAAAACGGCG ATATCAGCAG TGATCAGGTG CCGCGGCCCT ACATCGACCT TACCCATATC 
CCACGCAACC AGCTTCGAAT ATGCATTGCC GAAGACAATT CCATCAATGC TAAGATTGCC 
ATGCAGTATA TGCACAAGCT GGGTTATCCG AATGTTGATA CCTATGACAA CGGCCTGAAA 
GCAGTTGAGG GTCTGCGGGA GAAGGCGCGG CAGGGAAACC CGTATCACAT CATACTAATG 
GATGTACAAA TGCCAGTGCT GGATGGTTAT GAGGCGACGA AGATGTTGCG AAACGATCCG 
ATAGATTCTG TCCGCAATGT TCTCGTAATA GCCATGACAG CCTCGGCCAT TCAGGGCGAT 
CGAGAGAAGT GTCTCGCTGC GGGTATGAAC GATTATCTTG CCAAGCCGGT TCGAGGGGAG 
CTGCTGAAGA GAAAGCTGGA TACTTATCTG GGAGCGAGCG CGAGCATGAG TTTCAGCACT 
CAGCGTTTTC TCGAACGGCG CTTCGCCCAA AGGCCTGGAA GCCCCCAACC CATCAACCCC 
GGCAGCAGCA GTACCAAAAA AGACGTTGAC ACTCCACTTT CCTCGCCCGT GCCACCTCCT 
TTGGCTGATA ACACCGCTGC TTCTGCTTCC ACTGTCAAAA TCGGAGTGGG ATCCGCTGCG 
GCTTCTCTTC GAGAGGTGGC GGCCAGCAAC GTGAGCGGCG GTGAATCGAG ACCGTCTTCA 
CCCAAACTCC TCTCTCCCAG CGGCAAGAAC AGCAAACAAA GCCACAGAAG AGGCAGATCA 
TACGATATCC GCCTCCTTCG CCCCTCGGAG TCCGCAACAA GCTTCAGTAA CGTCACAAAT 
ACCAGCGGCG GAAGCAGCAG CAACAGCAAC AGCAGCAGCA AGAAGTTGGA CAGGCGTTTG 
TCCTCTCCAT CTTTGTCCTC CAGTGAGCGG CGAGGATCGG TGCCGGACCT GACAGCTGGT 
GGCGGCGGTG GTGATAAGCG GCAGCCTAAG AAGCTGGTGG AGGCTCGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU01823 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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