mak2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mak2
Entrez Gene ID	2542041
Full Name	histidine kinase Mak2
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019841.2	NP_594410.1	histidine kinase Mak2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mak3	NP_588379.1
Schizosaccharomyces pombe (fission yeast)	mak2	NP_594410.1
Magnaporthe oryzae (rice blast fungus)	MGG_06696	XP_003709405.1
Neurospora crassa	NCU01823	XP_955920.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate distinct functions for the three histidine kinases; Mak1 alone or Mak2 and Mak3 together were sufficient for the repression of the meiotic cycle when nutrients were available.
Title: Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans.

The following mak2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mak2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102503	NM_001019841.2 Latest version!	Schizosaccharomyces pombe histidine kinase Mak2 (mak2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mak2 ( NM_001019841.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594410.1 Schizosaccharomyces pombe histidine kinase Mak2 (mak2), mRNA.

Price & Availability↑

CloneID	OSc102503
Clone ID Related Accession (Same CDS sequence)	NM_001019841.2
Accession Version	NM_001019841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	histidine kinase Mak2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019841.1.

ATGAGCTTGT ACAAGTCATT GGACGTTGCA ATTGATTATG CAATTTCTCA GTTGGGAGAG 
TTCCAGTTCC AACCAATTCG TACGCAATCG AACCCTTCTT CTCTTCTTTC TGCGTGCTTG 
GTGCGTGCCG TCCATGTAGA AACAAGGCGA AAAGTAATTT TTAAGTTTTC CCAACAAACT 
TTCAAGCTAG AGAATGAATA CTTTTTGTTG CGTCAGTTGT CATCTCATCC AAATGGAAGA 
AATTATGCTA TTGCTCCCGC ATATATATTA CTGTTGAACG AAACCTTAGG TGCGCTTATT 
TACGATGATC CTGGGCCTAA CATTCTGGAT GAGTGGTTAG GGAATCCTAA CCCTTTGGAT 
CTAAAATTAT TTCTCAAGTT TGCCCTTGGC GTTTCTTACG TCTTATGTTT TTTACATGAA 
AAAAAAATCG TGCATGGCGA AATTCGTCTT GACACTTTCC ATTATGATTT GAATGCCCCT 
ATTCATGCAA AGTTGCTTAC CATCGGGAGT AGCGTATCCC CTATCAGATT TACCTTGTCT 
TCCTTAAACT GGAAGCGTCT TTATCAAGTC CAGAATATAT GTCACAAACT TCAGTTTTTT 
AGTCCTGAAC AAATTGGAAA TGTGGGGCGA CCGTTAGATT CCAGGTCCGA TATCTATTCT 
TTGGGTATTC TTTTTTATGT TATCTTGACC AAGCAATATC CCTGGGGTGG GCAATCTATG 
AGAATTGTTC AATCGATTCA TATGAGACAG TTTCCTTCTG TATTGCCTCG TCGTCCTGAT 
GCCTTTCCAG CGCTTGATCA ATTGATTCAA AAAATGACTG CCAAGTCTAT GAACTCAAGA 
ATTTCTTCTG CTACCGATTT GTGTTATACG ATTGTTGAGT TAATGCAAGA ATTTTCTACA 
ATCACCTCTT CTCCTTTGCT GGACCAAAAA TTGTTATCTA TAAATAAACC ACAGCAAGAA 
AAGCTTAAGT TTCCTAAATT ACTATTGACC AATTCTTCAG ATTACGTCCG GATCTTCCAC 
GAGCTTGTAG CTTTTTCTTC AAAACGCGAT CTTCTAACGA GTGCTAAGCG TGTTGATAAA 
CTTCCAAAGC AACACCTTTT CAAATATCGT CCAGTAGATA ATGAGGCTAC ATATTGCCAA 
GTTGTTACAG TTACCGGTGA GAAGGGCTCT GGAAAAAGTA ATTTGCTTAA TGCTGTCGCC 
GATGAAGCAA GAAAATTTGG ATATTTTGCA ATGAGCTCTT TTAAAGGTCA TCATTTTTCT 
CCATATTCTG CCATTTTTAA ATGTGTCTCT TTAATTATGC AACAGACTCT TCGTGAAGAA 
AAACAGCTAG TTACTGATTA CTTTACATCG CTGTGGGAAT TTCTTGGATT TCAATTGATT 
TACATGGGAG AACTATTTGA ATATGTTCCA GAATTAAACT CGCTACTATC TCCGAAATAT 
AATCTACATT GCAAAAGAGA AAACTATTTC AAGTTAAAAA AGAGAGATCC CCAACAATTC 
CGCAGTGCAA GCGGTCGTTT AGGATTTATG GTTTGTCTTC TAGAAATACT AAGCTTCACT 
TCCAGAGTTC GACCTGTCAT TATAATATTG GATGAATTAC ATTTGGCTGA TCATCCTTCG 
CTCTCTTTGA TAATTGGCAT GATTTCTCAT AGACTTCCTA TCTTACTAAT TTTGGCTTGG 
GATGAACCTG TGATGTTTAA AGATTTTTCG AAATGTCTTC ATGAGGCCCC ATATGCGATG 
GTCACTGATA TTAGAATGAA CCTTTTTGAT CGTAAAAATA TAACTGAATT TTTAGATAGC 
ACTTTAGAGT CTCCAACTCA AGCTTTGGGT CCGTTAGTGC TATTGATGCA AAAGCTTAGT 
AAGGGAAATC CGTTGGTGCT AAAAAGTCTT CTACTCATTG CCTTTGCTAA TAATGGCTTT 
GCCTTTCATC CAAAATCCTC TTCTTGGACT TATGATCTGC CTGTTATCAA CCGAAGCTTT 
GAAGCTCTTT CTTCTTATGA TATACCACCA TTACTGGCAT CATTGTTGGA TGCTTTACTC 
CCTGCCAGAT GTATTGAGTT TCTTTTATGG GCTGCATTGT TGGTCGAGCC GTTTCCGTTT 
GAATTGCTTC GATTAATTAC CACATCAATG CATTTGTTTA TCCCAAAAGA AGAGATATTG 
GATTTTCCTC TCAATGTTTT ACAATTTGAT AATGATAACG AAAGTTGTCA ATTTTCTGAA 
ACATTTTTTC GTGAGGGCAT ACTATCAAAA ATCAGTTTAA GAAGGGCCGA ATCAATGCAT 
GCCCAGATTG CTAAAGAATT AATCACTGGT ACTGCTAAGG AATTTTATGA TATCCGTACT 
GTGCATCACA TACTTAAAGG TTTAGGTGTT ATTAAAAAGT TCGATAATAC CAAGCCATAT 
ATATTGGCGC TAAAGGAATC AGCCGATGCT TTGATGCAAT TTGGTTCATA TGAGTATGCT 
ACGGAATTGT TGAAAAGTTG CCTATTCCTT TTACCTCGCA ACTTTTGGAA TAGCAAGTTG 
TACACAAGGA AAGATCTAAT TTCGATTCAC ATTAGCCTAG CCATGTGTTA TTGGTGGTCC 
AAAGATCATG AAAATGCTAT TAAAGTATTG AAAAATCCAA AGCTAAGCTC TTCAAATGTA 
TATGATTATT TGCCAGCATT CAGGTTATTA ACTAAGATCG AATACTACAA ATATCAATCC 
TTACGATCAA TTGATAAGGC GCAGGAACTT CTATCTAATT TAGGTCTGAA GTTGAAGGAA 
CCTACCGATG ATGTATTAAG GGAGTTTTAC GATAGACTTT CTACGAAATT TTTGGAATGC 
GATTTTCTAG TTAAGCAATC TGAGCCATTA GACCGAAAAA GAATTGATGC TATCAGCGTA 
ATTCTTTCGG AATGTGGATT TGTTCTTTTC AATTTCTCTC AACCTTACTA TTATTATTTC 
TCCTTTTTAC TTGCAGAAAT GTACTTAAGG TACGGAAATC CATCTTTAAG ATATAGTGTA 
ATGTTTTTGG CTTCTTATTG TTTTGTAACT AGAAGAAAAC CCGAATTTTT ACTTCGCATC 
TCACAAGTTG ATTCAGATTT GTTTGTAATT AAGGATCGCA GTGCGGTAGC CCATGCTGAA 
CTCATCTACT GGGGGCTTAA AAGAGAACTT TGTAGTACTG AAACTGGTTC AGCAGTTACA 
CTTGAAAGTA TACTATTACA ATGCGTTATG TTTGGTGATA AAATTTATGG AGCTTATTGT 
CTTGCCTGTC TAATGGCGCA ACGTGTCTTC CGTGGTGACC ATATTCACCA GTTATTACTT 
GATCAAGAAA ACTCAGAAAC ATTGCTTCTC CTATGGGATT GTGAGCCACC ATTCACATAT 
TATCTTATGC TCATACGAAA TTCACTTTTA GCTCTCTTTG GGTTAACAAA CAATGATGAT 
CCTAACAATA TCCTTACTAC AAAGCAAAGA ACTCAAAAAG ATCTTCACGA TAAACTTACG 
TCCAAGAAAG TTCCGTGTAC TTTTTGCTGT TGGTACTACG CTGGAATTAT TTTTCTTAAT 
ACTTTGTTTC ATCACTACGA GTATGTCATG TCAATTGCTC AAGAAGTTAG AAAATTGGTA 
GACGGCAAGC TGTATGAACG CTATTATTTG ATAACCCGTT CATTTATTGG CGTTGCGGCT 
TTACAACTTT TATTTTATAA AAAGAATATC TCGGAGTTTG AGCGTGAAAA AGTCGAGGAT 
GTGGCCCATT GGGCGCAATC TAGCTTGTCT GAAATGGCAA AATGTTTCCA TGCGGAGCTG 
TACAAGTTAT GGGTATGTCT TTTGGAGGGC TTGCGCCAAC GTAACCTTGG CAATTACATG 
GAGGCATTAA GACTTTTTGA GAAGGTCACA AGCATGGGTG CTTCGGTTTT TTCTCCCATT 
GAATTTCCAT TTGTGTTGGA ACTAATTGGG GAATTTTATT ATGGAAGGGG CCATAAGTTT 
CTCGCCAAGT CTTACATAAC TCGAGCGCTC AGTTGCCTTA AAAATATTGG TTGTTATGGG 
GTTGAAAATA AGTTGAGAAG TAGATATTCT GACTTAATTT CCGATGTTGA ATCTCGTGGA 
ACTACGGTTG TATCAATAGC AACTACCACT GGCGACTATG CTGAGAAGCT CAAACTTCTT 
AGGAATCAGG ACATTAACGA TTTTAGTCTA GGTCTTGCGT CTTATTCTGA TATTTTTGAT 
AAACCTCTGG TAACCTTGCC TGTGAAAAAA AGCAGTGCTG TTGATGAATC AGAAAATGAT 
TTTTACGACC GAAACGATGA GGAATCTTTT GACATTGTAT CTTTAGTTTC TGTTATAAAA 
TGTGGTCAAC TTTTATCGAG TAAATTAAGG TTAGGTCCTT TGCTTACAAC TGTCATAAAA 
CTAGTTATCG AATACTCTCA AGCCAAGCAT GCTGCTATAA TCTTGAAAGA CGCTTCAAAT 
TACACACTCG CTGCTCATGG CAATGTGGAG AAAGCCGAAT CATTTGAACC TCCTGTCATT 
TTGAGCCAAT CGGACGTCAA AATTCCAGAT TCTTTACTTT CCGAAGTATT TGACCATTGC 
CGAATCGTCT CACTGTACAC AGTTTCTGCT TCGCAAGATG CAGAGCTGTT AAGATGGTTG 
CAAGAAGAGC ATGATATGGA TTTTTTTGCC ATAATCCCCC TTCAATTTAA AGAATCGGTA 
ATAGGTGCTT TGTATCTATG TCTTTCGCGT AGAGCTATTC GTACAGGAAA TGTTACATTT 
TTGAAACTTT TGTCCCAGCA AATTGCAATT AGCGTTTCGA ATGCTTTACT TTTTCAGAGT 
TTGCGTCGCA CGATAACAGA TAATGTTACT CTTATCGAAC TTCAACGATT ATCATACCAA 
CGGTATAAGG CAATAGAGGA AAAATGCATA ACCCTTTTAG ACTCACTACC TTGTATAGTT 
TGGACGCTAG ATTCCGACAT TGGCGAAATA GAGTACACTA ATGCGTCGAA ACGGAATTAT 
TTTGGTGTTC CCGAAGATTG TCATGATTCA CTCAGTTGGA AAACATTCAT ACACCCGGAC 
CATCATCACC AATTTCAAGA AAAATTATTG AACCTTAAAA CTCTAGAGCT TGGCGACATT 
GAATTGCTTC TACGAATGGA AGATGGAAAT TACCATTGGC ATTTGTGTCG TGGATTGTCA 
TTTAAAGAAG ATGCTAATGC TAAAAAGTGG ATAGTTGTTT GTATAGATAT TAATGATGAA 
AAGGAAGCTC GTGAAGCTGC AATGCATGCT GTCAATCTAA AAACTAATTT TCTTGCCAAT 
ATGTCTCATG AACTGAGAAC TCCGTTTTCG AGTTTTTATG GGATGCTTTC TCTGCTTAGT 
GATACCAAAT TAAATGAAGA GCAGTATGAC ATAGTTAGCA CTGCTAAACA GAGTTGCACA 
TCGTTGGTCC AAATTATAGA TGATCTATTG AACTTCAGCG AATTGAAGTC AGGCAAAATG 
AAACTTGAGC CTGACAAAGT CTTTGATGTT GAAGAGAATA TTGCAGATTG CATTGAGTTA 
GTATACCCTT CTCTTTCTTC TAAACCTGTT CAAATTTCAT ACGACATATA TCCGAATGTT 
CCAGCTTTAT TGGCTGGTGA TTCTGCAAAG CTTCGACAAG TTATTACCAA TCTCCTTGGA 
AATTCCGTAA AGTTTACAAC GGAGGGTCAT ATTTTGTTAC GTTGTATGGC TATTGATGAG 
GAAATAAATG CAGAAGAAAA TCAATGCAAA TTGAGATTTG AGATTGAGGA CACTGGAATT 
GGACTTAAAG AAGAGCAACT TAAACTGCTT TTTAATCCTT TCACTCAAGT CGATGGTAGC 
ACTACTAGAA TCTATGGAGG TTCAGGCCTT GGGCTCTCTA TTTGCCTTCA AATATGCAAA 
ATAATGGATG GAGACATCGG TGTTCAGTCT GTTTATGGAG AAGGTTCTAC ATTCTGGTTC 
CATGTCCAAT TGCGTAACGT TACTTCTAAG TTATCTCAGA AACATTTCGA AGAAAGCCAT 
GAGAGATTTG CTAATATTCG ACAATCTCTT AAGAATGCTA AAATACTTGT AGTTAAATCA 
TTTACTACAT CACGATCTAT TTTCAGGTCT CTTTTCTCCT TAGCTGTAGT TGATACAACT 
ACTATTTACA GTGATATCGA ACAGCAGTTA ATTGATTCTT TAGATAAGCG ACAACCTTAT 
GACTTTCTTT GTATCGAAGC TGCCAGCGGC CAGACGGAAC AAATAATTAC TCAGATACTT 
AGTAATCAAA AATTGAACAA GGTATTACTT ATTGTTCTGT TACCGTCGAT TCAACGAACG 
AAAGTACGAT CTGACGGCGA TCCATTCATA ACCTCTTTAA ATAAAAACCA AAGCAGAATA 
TTTTGCTTCA GGGAGCCAAT ACGCATTTCA AAGTTACTAC AAAACTTTCC CGCATTACTA 
AGTAAATGGT CAACTCCTAC CAAACTTGTC GAGCCCTCTC AATTTCGAGC ATCACCTAGG 
AAGGTCGATC AAGCAGTTGT TCTTTCTAGC GAAGAGAAGG AGATTCTTCA GAAAAAGTAT 
GCGCTAATAG CTGAAGATAA TTTGATTGCT AGAAAGTTGC TCACGAAACA ATTAAGCAAT 
TTAGGATTCC AAGTTCATGC CGCGGTAGAT GGCGTTGAAT TGGTTAAAAT GTATGAGGCT 
AAACAATTTG GTTTTTATAG TGTAATATTT GCTGATTACC ATATGCCCAT CCGAGATGGT 
GCAGAAGCAG TTATGGATAT CCGTGCTTAC GAACGTGAAA ATAATTGCTC AACTCCAATC 
CCAGTCATTG CTTTAACAGC TGATATACAG AAATCTGCAA AACAGCGATG CTTAGAGGTT 
GGAATGAATT TTTATTTGAC CAAACCATTT ACTCAAAAAC AATTAGTCAA TGCCGTTCGC 
GAATTTGTGC TTTTGGAGAA GAGTGCTCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594410.1
CDS	113..7045
Translation	MSLYKSLDVAIDYAISQLGEFQFQPIRTQSNPSSLLSACLVRAVHVETRRKVIFKFSQQTFKLENEYFLLRQLSSHPNGRNYAIAPAYILLLNETLGALIYDDPGPNILDEWLGNPNPLDLKLFLKFALGVSYVLCFLHEKKIVHGEIRLDTFHYDLNAPIHAKLLTIGSSVSPIRFTLSSLNWKRLYQVQNICHKLQFFSPEQIGNVGRPLDSRSDIYSLGILFYVILTKQYPWGGQSMRIVQSIHMRQFPSVLPRRPDAFPALDQLIQKMTAKSMNSRISSATDLCYTIVELMQEFSTITSSPLLDQKLLSINKPQQEKLKFPKLLLTNSSDYVRIFHELVAFSSKRDLLTSAKRVDKLPKQHLFKYRPVDNEATYCQVVTVTGEKGSGKSNLLNAVADEARKFGYFAMSSFKGHHFSPYSAIFKCVSLIMQQTLREEKQLVTDYFTSLWEFLGFQLIYMGELFEYVPELNSLLSPKYNLHCKRENYFKLKKRDPQQFRSASGRLGFMVCLLEILSFTSRVRPVIIILDELHLADHPSLSLIIGMISHRLPILLILAWDEPVMFKDFSKCLHEAPYAMVTDIRMNLFDRKNITEFLDSTLESPTQALGPLVLLMQKLSKGNPLVLKSLLLIAFANNGFAFHPKSSSWTYDLPVINRSFEALSSYDIPPLLASLLDALLPARCIEFLLWAALLVEPFPFELLRLITTSMHLFIPKEEILDFPLNVLQFDNDNESCQFSETFFREGILSKISLRRAESMHAQIAKELITGTAKEFYDIRTVHHILKGLGVIKKFDNTKPYILALKESADALMQFGSYEYATELLKSCLFLLPRNFWNSKLYTRKDLISIHISLAMCYWWSKDHENAIKVLKNPKLSSSNVYDYLPAFRLLTKIEYYKYQSLRSIDKAQELLSNLGLKLKEPTDDVLREFYDRLSTKFLECDFLVKQSEPLDRKRIDAISVILSECGFVLFNFSQPYYYYFSFLLAEMYLRYGNPSLRYSVMFLASYCFVTRRKPEFLLRISQVDSDLFVIKDRSAVAHAELIYWGLKRELCSTETGSAVTLESILLQCVMFGDKIYGAYCLACLMAQRVFRGDHIHQLLLDQENSETLLLLWDCEPPFTYYLMLIRNSLLALFGLTNNDDPNNILTTKQRTQKDLHDKLTSKKVPCTFCCWYYAGIIFLNTLFHHYEYVMSIAQEVRKLVDGKLYERYYLITRSFIGVAALQLLFYKKNISEFEREKVEDVAHWAQSSLSEMAKCFHAELYKLWVCLLEGLRQRNLGNYMEALRLFEKVTSMGASVFSPIEFPFVLELIGEFYYGRGHKFLAKSYITRALSCLKNIGCYGVENKLRSRYSDLISDVESRGTTVVSIATTTGDYAEKLKLLRNQDINDFSLGLASYSDIFDKPLVTLPVKKSSAVDESENDFYDRNDEESFDIVSLVSVIKCGQLLSSKLRLGPLLTTVIKLVIEYSQAKHAAIILKDASNYTLAAHGNVEKAESFEPPVILSQSDVKIPDSLLSEVFDHCRIVSLYTVSASQDAELLRWLQEEHDMDFFAIIPLQFKESVIGALYLCLSRRAIRTGNVTFLKLLSQQIAISVSNALLFQSLRRTITDNVTLIELQRLSYQRYKAIEEKCITLLDSLPCIVWTLDSDIGEIEYTNASKRNYFGVPEDCHDSLSWKTFIHPDHHHQFQEKLLNLKTLELGDIELLLRMEDGNYHWHLCRGLSFKEDANAKKWIVVCIDINDEKEAREAAMHAVNLKTNFLANMSHELRTPFSSFYGMLSLLSDTKLNEEQYDIVSTAKQSCTSLVQIIDDLLNFSELKSGKMKLEPDKVFDVEENIADCIELVYPSLSSKPVQISYDIYPNVPALLAGDSAKLRQVITNLLGNSVKFTTEGHILLRCMAIDEEINAEENQCKLRFEIEDTGIGLKEEQLKLLFNPFTQVDGSTTRIYGGSGLGLSICLQICKIMDGDIGVQSVYGEGSTFWFHVQLRNVTSKLSQKHFEESHERFANIRQSLKNAKILVVKSFTTSRSIFRSLFSLAVVDTTTIYSDIEQQLIDSLDKRQPYDFLCIEAASGQTEQIITQILSNQKLNKVLLIVLLPSIQRTKVRSDGDPFITSLNKNQSRIFCFREPIRISKLLQNFPALLSKWSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIARKLLTKQLSNLGFQVHAAVDGVELVKMYEAKQFGFYSVIFADYHMPIRDGAEAVMDIRAYERENNCSTPIPVIALTADIQKSAKQRCLEVGMNFYLTKPFTQKQLVNAVREFVLLEKSAR

Target ORF information:

RefSeq Version	NM_001019841.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe histidine kinase Mak2 (mak2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019841.2

ORF Insert Sequence:

ATGAGCTTGT ACAAGTCATT GGACGTTGCA ATTGATTATG CAATTTCTCA GTTGGGAGAG 
TTCCAGTTCC AACCAATTCG TACGCAATCG AACCCTTCTT CTCTTCTTTC TGCGTGCTTG 
GTGCGTGCCG TCCATGTAGA AACAAGGCGA AAAGTAATTT TTAAGTTTTC CCAACAAACT 
TTCAAGCTAG AGAATGAATA CTTTTTGTTG CGTCAGTTGT CATCTCATCC AAATGGAAGA 
AATTATGCTA TTGCTCCCGC ATATATATTA CTGTTGAACG AAACCTTAGG TGCGCTTATT 
TACGATGATC CTGGGCCTAA CATTCTGGAT GAGTGGTTAG GGAATCCTAA CCCTTTGGAT 
CTAAAATTAT TTCTCAAGTT TGCCCTTGGC GTTTCTTACG TCTTATGTTT TTTACATGAA 
AAAAAAATCG TGCATGGCGA AATTCGTCTT GACACTTTCC ATTATGATTT GAATGCCCCT 
ATTCATGCAA AGTTGCTTAC CATCGGGAGT AGCGTATCCC CTATCAGATT TACCTTGTCT 
TCCTTAAACT GGAAGCGTCT TTATCAAGTC CAGAATATAT GTCACAAACT TCAGTTTTTT 
AGTCCTGAAC AAATTGGAAA TGTGGGGCGA CCGTTAGATT CCAGGTCCGA TATCTATTCT 
TTGGGTATTC TTTTTTATGT TATCTTGACC AAGCAATATC CCTGGGGTGG GCAATCTATG 
AGAATTGTTC AATCGATTCA TATGAGACAG TTTCCTTCTG TATTGCCTCG TCGTCCTGAT 
GCCTTTCCAG CGCTTGATCA ATTGATTCAA AAAATGACTG CCAAGTCTAT GAACTCAAGA 
ATTTCTTCTG CTACCGATTT GTGTTATACG ATTGTTGAGT TAATGCAAGA ATTTTCTACA 
ATCACCTCTT CTCCTTTGCT GGACCAAAAA TTGTTATCTA TAAATAAACC ACAGCAAGAA 
AAGCTTAAGT TTCCTAAATT ACTATTGACC AATTCTTCAG ATTACGTCCG GATCTTCCAC 
GAGCTTGTAG CTTTTTCTTC AAAACGCGAT CTTCTAACGA GTGCTAAGCG TGTTGATAAA 
CTTCCAAAGC AACACCTTTT CAAATATCGT CCAGTAGATA ATGAGGCTAC ATATTGCCAA 
GTTGTTACAG TTACCGGTGA GAAGGGCTCT GGAAAAAGTA ATTTGCTTAA TGCTGTCGCC 
GATGAAGCAA GAAAATTTGG ATATTTTGCA ATGAGCTCTT TTAAAGGTCA TCATTTTTCT 
CCATATTCTG CCATTTTTAA ATGTGTCTCT TTAATTATGC AACAGACTCT TCGTGAAGAA 
AAACAGCTAG TTACTGATTA CTTTACATCG CTGTGGGAAT TTCTTGGATT TCAATTGATT 
TACATGGGAG AACTATTTGA ATATGTTCCA GAATTAAACT CGCTACTATC TCCGAAATAT 
AATCTACATT GCAAAAGAGA AAACTATTTC AAGTTAAAAA AGAGAGATCC CCAACAATTC 
CGCAGTGCAA GCGGTCGTTT AGGATTTATG GTTTGTCTTC TAGAAATACT AAGCTTCACT 
TCCAGAGTTC GACCTGTCAT TATAATATTG GATGAATTAC ATTTGGCTGA TCATCCTTCG 
CTCTCTTTGA TAATTGGCAT GATTTCTCAT AGACTTCCTA TCTTACTAAT TTTGGCTTGG 
GATGAACCTG TGATGTTTAA AGATTTTTCG AAATGTCTTC ATGAGGCCCC ATATGCGATG 
GTCACTGATA TTAGAATGAA CCTTTTTGAT CGTAAAAATA TAACTGAATT TTTAGATAGC 
ACTTTAGAGT CTCCAACTCA AGCTTTGGGT CCGTTAGTGC TATTGATGCA AAAGCTTAGT 
AAGGGAAATC CGTTGGTGCT AAAAAGTCTT CTACTCATTG CCTTTGCTAA TAATGGCTTT 
GCCTTTCATC CAAAATCCTC TTCTTGGACT TATGATCTGC CTGTTATCAA CCGAAGCTTT 
GAAGCTCTTT CTTCTTATGA TATACCACCA TTACTGGCAT CATTGTTGGA TGCTTTACTC 
CCTGCCAGAT GTATTGAGTT TCTTTTATGG GCTGCATTGT TGGTCGAGCC GTTTCCGTTT 
GAATTGCTTC GATTAATTAC CACATCAATG CATTTGTTTA TCCCAAAAGA AGAGATATTG 
GATTTTCCTC TCAATGTTTT ACAATTTGAT AATGATAACG AAAGTTGTCA ATTTTCTGAA 
ACATTTTTTC GTGAGGGCAT ACTATCAAAA ATCAGTTTAA GAAGGGCCGA ATCAATGCAT 
GCCCAGATTG CTAAAGAATT AATCACTGGT ACTGCTAAGG AATTTTATGA TATCCGTACT 
GTGCATCACA TACTTAAAGG TTTAGGTGTT ATTAAAAAGT TCGATAATAC CAAGCCATAT 
ATATTGGCGC TAAAGGAATC AGCCGATGCT TTGATGCAAT TTGGTTCATA TGAGTATGCT 
ACGGAATTGT TGAAAAGTTG CCTATTCCTT TTACCTCGCA ACTTTTGGAA TAGCAAGTTG 
TACACAAGGA AAGATCTAAT TTCGATTCAC ATTAGCCTAG CCATGTGTTA TTGGTGGTCC 
AAAGATCATG AAAATGCTAT TAAAGTATTG AAAAATCCAA AGCTAAGCTC TTCAAATGTA 
TATGATTATT TGCCAGCATT CAGGTTATTA ACTAAGATCG AATACTACAA ATATCAATCC 
TTACGATCAA TTGATAAGGC GCAGGAACTT CTATCTAATT TAGGTCTGAA GTTGAAGGAA 
CCTACCGATG ATGTATTAAG GGAGTTTTAC GATAGACTTT CTACGAAATT TTTGGAATGC 
GATTTTCTAG TTAAGCAATC TGAGCCATTA GACCGAAAAA GAATTGATGC TATCAGCGTA 
ATTCTTTCGG AATGTGGATT TGTTCTTTTC AATTTCTCTC AACCTTACTA TTATTATTTC 
TCCTTTTTAC TTGCAGAAAT GTACTTAAGG TACGGAAATC CATCTTTAAG ATATAGTGTA 
ATGTTTTTGG CTTCTTATTG TTTTGTAACT AGAAGAAAAC CCGAATTTTT ACTTCGCATC 
TCACAAGTTG ATTCAGATTT GTTTGTAATT AAGGATCGCA GTGCGGTAGC CCATGCTGAA 
CTCATCTACT GGGGGCTTAA AAGAGAACTT TGTAGTACTG AAACTGGTTC AGCAGTTACA 
CTTGAAAGTA TACTATTACA ATGCGTTATG TTTGGTGATA AAATTTATGG AGCTTATTGT 
CTTGCCTGTC TAATGGCGCA ACGTGTCTTC CGTGGTGACC ATATTCACCA GTTATTACTT 
GATCAAGAAA ACTCAGAAAC ATTGCTTCTC CTATGGGATT GTGAGCCACC ATTCACATAT 
TATCTTATGC TCATACGAAA TTCACTTTTA GCTCTCTTTG GGTTAACAAA CAATGATGAT 
CCTAACAATA TCCTTACTAC AAAGCAAAGA ACTCAAAAAG ATCTTCACGA TAAACTTACG 
TCCAAGAAAG TTCCGTGTAC TTTTTGCTGT TGGTACTACG CTGGAATTAT TTTTCTTAAT 
ACTTTGTTTC ATCACTACGA GTATGTCATG TCAATTGCTC AAGAAGTTAG AAAATTGGTA 
GACGGCAAGC TGTATGAACG CTATTATTTG ATAACCCGTT CATTTATTGG CGTTGCGGCT 
TTACAACTTT TATTTTATAA AAAGAATATC TCGGAGTTTG AGCGTGAAAA AGTCGAGGAT 
GTGGCCCATT GGGCGCAATC TAGCTTGTCT GAAATGGCAA AATGTTTCCA TGCGGAGCTG 
TACAAGTTAT GGGTATGTCT TTTGGAGGGC TTGCGCCAAC GTAACCTTGG CAATTACATG 
GAGGCATTAA GACTTTTTGA GAAGGTCACA AGCATGGGTG CTTCGGTTTT TTCTCCCATT 
GAATTTCCAT TTGTGTTGGA ACTAATTGGG GAATTTTATT ATGGAAGGGG CCATAAGTTT 
CTCGCCAAGT CTTACATAAC TCGAGCGCTC AGTTGCCTTA AAAATATTGG TTGTTATGGG 
GTTGAAAATA AGTTGAGAAG TAGATATTCT GACTTAATTT CCGATGTTGA ATCTCGTGGA 
ACTACGGTTG TATCAATAGC AACTACCACT GGCGACTATG CTGAGAAGCT CAAACTTCTT 
AGGAATCAGG ACATTAACGA TTTTAGTCTA GGTCTTGCGT CTTATTCTGA TATTTTTGAT 
AAACCTCTGG TAACCTTGCC TGTGAAAAAA AGCAGTGCTG TTGATGAATC AGAAAATGAT 
TTTTACGACC GAAACGATGA GGAATCTTTT GACATTGTAT CTTTAGTTTC TGTTATAAAA 
TGTGGTCAAC TTTTATCGAG TAAATTAAGG TTAGGTCCTT TGCTTACAAC TGTCATAAAA 
CTAGTTATCG AATACTCTCA AGCCAAGCAT GCTGCTATAA TCTTGAAAGA CGCTTCAAAT 
TACACACTCG CTGCTCATGG CAATGTGGAG AAAGCCGAAT CATTTGAACC TCCTGTCATT 
TTGAGCCAAT CGGACGTCAA AATTCCAGAT TCTTTACTTT CCGAAGTATT TGACCATTGC 
CGAATCGTCT CACTGTACAC AGTTTCTGCT TCGCAAGATG CAGAGCTGTT AAGATGGTTG 
CAAGAAGAGC ATGATATGGA TTTTTTTGCC ATAATCCCCC TTCAATTTAA AGAATCGGTA 
ATAGGTGCTT TGTATCTATG TCTTTCGCGT AGAGCTATTC GTACAGGAAA TGTTACATTT 
TTGAAACTTT TGTCCCAGCA AATTGCAATT AGCGTTTCGA ATGCTTTACT TTTTCAGAGT 
TTGCGTCGCA CGATAACAGA TAATGTTACT CTTATCGAAC TTCAACGATT ATCATACCAA 
CGGTATAAGG CAATAGAGGA AAAATGCATA ACCCTTTTAG ACTCACTACC TTGTATAGTT 
TGGACGCTAG ATTCCGACAT TGGCGAAATA GAGTACACTA ATGCGTCGAA ACGGAATTAT 
TTTGGTGTTC CCGAAGATTG TCATGATTCA CTCAGTTGGA AAACATTCAT ACACCCGGAC 
CATCATCACC AATTTCAAGA AAAATTATTG AACCTTAAAA CTCTAGAGCT TGGCGACATT 
GAATTGCTTC TACGAATGGA AGATGGAAAT TACCATTGGC ATTTGTGTCG TGGATTGTCA 
TTTAAAGAAG ATGCTAATGC TAAAAAGTGG ATAGTTGTTT GTATAGATAT TAATGATGAA 
AAGGAAGCTC GTGAAGCTGC AATGCATGCT GTCAATCTAA AAACTAATTT TCTTGCCAAT 
ATGTCTCATG AACTGAGAAC TCCGTTTTCG AGTTTTTATG GGATGCTTTC TCTGCTTAGT 
GATACCAAAT TAAATGAAGA GCAGTATGAC ATAGTTAGCA CTGCTAAACA GAGTTGCACA 
TCGTTGGTCC AAATTATAGA TGATCTATTG AACTTCAGCG AATTGAAGTC AGGCAAAATG 
AAACTTGAGC CTGACAAAGT CTTTGATGTT GAAGAGAATA TTGCAGATTG CATTGAGTTA 
GTATACCCTT CTCTTTCTTC TAAACCTGTT CAAATTTCAT ACGACATATA TCCGAATGTT 
CCAGCTTTAT TGGCTGGTGA TTCTGCAAAG CTTCGACAAG TTATTACCAA TCTCCTTGGA 
AATTCCGTAA AGTTTACAAC GGAGGGTCAT ATTTTGTTAC GTTGTATGGC TATTGATGAG 
GAAATAAATG CAGAAGAAAA TCAATGCAAA TTGAGATTTG AGATTGAGGA CACTGGAATT 
GGACTTAAAG AAGAGCAACT TAAACTGCTT TTTAATCCTT TCACTCAAGT CGATGGTAGC 
ACTACTAGAA TCTATGGAGG TTCAGGCCTT GGGCTCTCTA TTTGCCTTCA AATATGCAAA 
ATAATGGATG GAGACATCGG TGTTCAGTCT GTTTATGGAG AAGGTTCTAC ATTCTGGTTC 
CATGTCCAAT TGCGTAACGT TACTTCTAAG TTATCTCAGA AACATTTCGA AGAAAGCCAT 
GAGAGATTTG CTAATATTCG ACAATCTCTT AAGAATGCTA AAATACTTGT AGTTAAATCA 
TTTACTACAT CACGATCTAT TTTCAGGTCT CTTTTCTCCT TAGCTGTAGT TGATACAACT 
ACTATTTACA GTGATATCGA ACAGCAGTTA ATTGATTCTT TAGATAAGCG ACAACCTTAT 
GACTTTCTTT GTATCGAAGC TGCCAGCGGC CAGACGGAAC AAATAATTAC TCAGATACTT 
AGTAATCAAA AATTGAACAA GGTATTACTT ATTGTTCTGT TACCGTCGAT TCAACGAACG 
AAAGTACGAT CTGACGGCGA TCCATTCATA ACCTCTTTAA ATAAAAACCA AAGCAGAATA 
TTTTGCTTCA GGGAGCCAAT ACGCATTTCA AAGTTACTAC AAAACTTTCC CGCATTACTA 
AGTAAATGGT CAACTCCTAC CAAACTTGTC GAGCCCTCTC AATTTCGAGC ATCACCTAGG 
AAGGTCGATC AAGCAGTTGT TCTTTCTAGC GAAGAGAAGG AGATTCTTCA GAAAAAGTAT 
GCGCTAATAG CTGAAGATAA TTTGATTGCT AGAAAGTTGC TCACGAAACA ATTAAGCAAT 
TTAGGATTCC AAGTTCATGC CGCGGTAGAT GGCGTTGAAT TGGTTAAAAT GTATGAGGCT 
AAACAATTTG GTTTTTATAG TGTAATATTT GCTGATTACC ATATGCCCAT CCGAGATGGT 
GCAGAAGCAG TTATGGATAT CCGTGCTTAC GAACGTGAAA ATAATTGCTC AACTCCAATC 
CCAGTCATTG CTTTAACAGC TGATATACAG AAATCTGCAA AACAGCGATG CTTAGAGGTT 
GGAATGAATT TTTATTTGAC CAAACCATTT ACTCAAAAAC AATTAGTCAA TGCCGTTCGC 
GAATTTGTGC TTTTGGAGAA GAGTGCTCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mak2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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