NCU02510 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU02510
Entrez Gene ID	3881791
Full Name	clathrin heavy chain
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_960548.2	XP_965641.1	clathrin heavy chain

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cltca	NP_001005391.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003021	XP_311856.3
Magnaporthe oryzae (rice blast fungus)	MGG_07768	XP_003712981.1
Homo sapiens (human)	CLTC	NP_001275582.1
Bos taurus (cattle)	CLTC	NP_776448.1
Mus musculus (house mouse)	Cltc	NP_001003908.1
Schizosaccharomyces pombe (fission yeast)	chc1	NP_594148.1
Arabidopsis thaliana (thale cress)	AT3G08530	NP_187466.4
Kluyveromyces lactis	KLLA0F09911g	XP_455531.1
Eremothecium gossypii	AGOS_AER359W	NP_985215.2
Xenopus tropicalis (tropical clawed frog)	cltc	NP_001011039.1
Pan troglodytes (chimpanzee)	CLTC	XP_001136053.1
	CLTC	XP_001108647.2
Gallus gallus (chicken)	CLTC	NP_001073586.1
Danio rerio (zebrafish)	cltcb	NP_001181907.1
Drosophila melanogaster (fruit fly)	Chc	NP_996451.1
Saccharomyces cerevisiae (baker's yeast)	CHC1	NP_011309.1
Arabidopsis thaliana (thale cress)	AT3G11130	NP_187724.2
Canis lupus familiaris (dog)	CLTC	XP_537700.2
Rattus norvegicus (Norway rat)	Cltc	NP_062172.1
Caenorhabditis elegans (roundworm)	chc-1	NP_499260.1
Neurospora crassa	NCU02510	XP_965641.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU02510 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02510 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf08883	XM_960548.2 Latest version!	Neurospora crassa OR74A clathrin heavy chain (NCU02510), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU02510 ( XM_960548.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_965641.1 Neurospora crassa OR74A clathrin heavy chain (NCU02510), mRNA.

Price & Availability↑

CloneID	ONf08883
Clone ID Related Accession (Same CDS sequence)	XM_960548.2
Accession Version	XM_960548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	clathrin heavy chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960548.1.

ATGGCTCAGG CCCTACCCAT CAAATTCCAG GAGCTGCTGC AACTCAGCGC CCTCGGCATC 
AACCAGACTG CCATCACTTT CAATACCTGT ACTCTAGAAT CGGACAACTG GGTGTGTATT 
CGCGACAAGA AGGACGAGGC AGCGTCCCCT GAAGTCATCA TCGTCGATCT CAAGAACGGC 
AATAATGTTA TCCGACGTCC CATCAAGGCC GACAGTGCCA TAATGCACTG GACGCGCCAA 
GTAATTGCCC TCAAGGCCCA GGCGCGCACA CTCCAGATTT TCGACCTCGA AAACAAGGCC 
AAGCTCAAGT CGACAAACAT GAGCGAGGAT GTCTTGTTCT GGAAGTGGGT TAGCGAGACC 
ACGCTTGGCT TGATCACAGA TGGCTCTGTT TACCATTGGG ATGTCTTCGA CCCGAACCAG 
CCCGCTCCGG TCAAGGTCTT CGATCGTAAT GCTAATCTCC AGGGCAACCA AATAATTAAT 
TATCGGACCA GCGCCGACGG AAAGTGGATG GTTGTTGTCG GTATCTCACA ACAGCAGGGC 
CGCGTTGTTG GCGCCATGCA GCTCTATTCC AAAGACCGCG GTATCAGCCA GGCTATTGAG 
GGTCATGCCG CTGCTTTTGG CACCCTCCGA CTAGAGGGCG CCCCGGAGGA CACCAAGGTG 
TTTACCTTTG CGGTCCGCAC CGCCGTCGGC GCGAAGCTCC ACATTGTCGA GGTCGACCAT 
CCCGAAACAA ACCCCGTCTT CCCCAAGAAG GCCGTGGACG TCTTCTTCCC TCCCGAGGCC 
AGCAATGACT TCCCCGTCGC CCTCCAGGTT TCGCAAAAGT ACGGCATCAT CTACCTTATC 
ACCAAGTACG GTTTCATCCA CCTCTACGAC CTCGAGACTG CCACATGTAT CTTCATGAAC 
CGCATCTCAA GCGATACTAT CTTCACTGCT TGCGGTGACA AGGATTCGAC CGGCGTCATT 
GGCATCAACC GCAAGGGACA GGTTCTCTTC GTCAGCGCCG ATGAGAACAC AATCGTTCCT 
TACGTCCTTG AGGCACACGG CAATGAGCTC GCTATCAAGT TGGCTTCTCG TGCAGGGCTC 
CCAGGCGCCG ATAACCTCTA CCAGCAACGC TTCGAGCAAC TCTTCAGCAA TGGCAACTAC 
CAAGAGGCCG CCAAGGTGGC TGCCAACTCT CCTCGAGGTT TCCTTCGCAC TCCTCAGACC 
ATCGAGCGCT TCAAGCGTCT TCCACAGCAG CCTGGACAGA TGTCGCATAT CTTGCAGTAC 
TTTGGTATGC TTCTAGACAA GGGTTCGCTG AACGAGCATG AGACCATTGA GCTTGCCCAA 
CCCGTTCTCG CTCAGAACCG CAAACAGCTA TTGCAGAAGT GGCTCAGCGA GAACAAGCTC 
GAGTGCTCTG AGAGACTTGG TGACATGGTC CGGCCCCACG ACATTAACAT GGCGCTTGCC 
ATCTATTTGA AGGCCAACGC TCCTCACAAG GTTGTTGCTG GGTTTGCCGA GACTGGCCAG 
TTTGAGAAGA TTCTGCCCTA CTGCGGTCAA GCCAACTACC AGCCTGACTT TATTCAGCTT 
CTCCACCACA TTGTCCGTGT CAATGCTGAG AAGGGTGCCG AGTTTGCCAC GACGCTTGCC 
AATCACGAAG GCGGCCCGCT GGTCGACTTC GAGAAGGTGG TTGACATCTT CCAGTCTCAA 
GGAATGATCC AGCAAGCAAC TGCTTTCCTT CTTGACGCTC TGAAGGATAA CAAGCCCGAG 
CATGCCCACC TTCAGACTCG TCTATTGGAG ATGAACCTCG TCAACGCCCC CCAGGTTGCT 
GACGCCATTC TTGGCAACGA CATGTTCTCC CACTTTGACA AGGCGCACAT TGCGAACCTC 
TGCGAGCAAG CAGGTCTTTT GCAGAAGGCC CTGGAGCTCT ATGAGGATCC TGCGTCCATC 
AAGCGGGTTA TTGTCAACAT CCCTGGAATG CCTAACTACA ACCCCGAGTG GTTGATTGAG 
TATTTCAAGG CGCTGTCTGT TGAGCAATCG CTTGACTGCC TGGACGCCAT GATGAAGCAC 
AATATTCGCC AGAATCTCCA GACTGTCGTT CAAGTCGCAA CCAAGTATGC GGAGCTCCTC 
GGCGCGCAGC AATTGATTGA TCTCTTTGAG AAGTACAAGA CTGCTGAAGG TCTTTACTAC 
TTCCTTGCCA GTGTCGTCAA TGTCACCGAG GACCCCGAAG TCGTATTCAA GTACATTGAG 
GCTGCCACCA AGATGGGACA AATTCGCGAG GTTGAGCGTA TCTGCCGCGA CAACAGCGTT 
TACAACCCCG AGAAGGTGAA GAACTTCCTC AAGGAGGCCA AGCTCAGCGA GATGCTTCCT 
TTGATGGTTG TCTGCGATCG CTTCAACTTT GTTCATGACC TGGTTCTCTA CCTGTACCAG 
CACCAGCAGT TCAAGTCCAT TGAGATTTAC GTGCAGCAGG TCAACCCGAG CAGGACTCCT 
GGTGTCATCG GAGGACTGCT CGATGTGGAC TGTGATGAAA GCATCATCAG GAATCTCCTA 
AGCACGGTCA ACCCCGCATC TATCCCTATC GATGAGCTGG TGCAGGAGGT TGAGACTCGT 
AACCGCCTTA AGCTACTCCT GCCCTTCCTG GAGGCTACCC TCGCTGCCGG TAACCAGCAG 
CAGGCTGTTT ACAACGCTCT CGCCAAGATA TACATTGACT CCAACAACAA CCCAGAGAAG 
TTCCTCAAGG AGAACGATCA GTATGATACC CTCACTGTTG GAAAGTACTG CGAGAAGCGT 
GATCCCAACT TGGCCTATAT TGCCTATCGC AAGGGCCAGA ATGATCTCGA ACTCGTGAAC 
ATCACCAACG AGAACCAGAT GTACAAGGCT CAGGCGAGGT ATCTTTTGGA GCGCGCTGAT 
AGGGAACTTT GGATGTTTGT GCTCAGCGAG AACAACGTTC ACCGCCGTTC TGTCGTAGAC 
CAAGTCATCT CGACGGCCGT TCCTGAGTCG ACTGATCCCG CCAAGGTTTC CGAGGCTGTT 
ACGTGCTTCC TGAACGCCGA CTTGCCTGGC GAGCTTATTG AGCTGCTGGA GAAGATTGTC 
CTGGAGCCGT CGCCTTTCAG CGACAACCAG AACCTGCAGA ACTTGCTTAT CTTCACTGCC 
GCCAAGGCTG ACAAGGCCCG TGTGATGGAC TACATTCACA AGCTCGACAA CTTCGCCGCC 
GACGAGATTG CTAATGTTTG TATTGAGGTT GGCCTGCACG AGGAAGCCTT TGAGGTCTAC 
AAGAAGATTG ACAACAAGGA GGCCGCTGTC AATGTTCTTG TTGAGCACGT CGTTAGCATT 
GACCGTGCTC AAGCATACGC CGAGGAGGTT GATATTCCTC AGGTTTGGAG CAAAGTTGCC 
AAGGCTCAGC TTGATGGTCT CCGCGTCAGC GATTCCATTG AATCTTACAT CAAGGCCGAG 
GATCCGAAGA ACTACGAGGA AGTGATCGAG GTTGCCGTTG CTGCTGGAAA GAACGAAGAG 
CTCATCAAGT ACCTCCGTAT GGCCCGCAAG ACCCTTCGGG AGCCTGTCAT TGACACTGCT 
CTTGCTTTCT GCTACGCCCG CCTCGATCAA CTTCCTGAGC TTGAAGAGTT CCTGCGTGCT 
ACCAATGTTG CCAATGTTGA GGAGTCTGGT GACAAGGCGT ACGCCGAGGG CTTCTTTGAG 
GCGGCCAAAA TCTTCTACAC CAGCATTTCC AACTGGGCTA AGCTGGCCAC TACTCTTGTC 
CACCTGTCCG ACTACCAGGC GGCTGTGGAC TGCGCCCGCA AGGCCAACAA CATCAAGGTG 
TGGAAGGAGG TCCACGAGGC CTGCGTCAAC AAGAAGGAGT TCCGTCTTGC TCAGATTTGC 
GGTCTCAACC TTATTGTTGA TGCTGAGCAA CTCCAGGCTC TTGTCAAGCA GTACGAGACA 
AACGGCTACT TTGATGAGCT CATCAGCTTG CTCGAGCAGG GTCTTGGATT GGAGCGCGCC 
CACATGGGAA TGTTCACCGA GTTGGGTATT GCGCTGTCTA AATACCACCC CGAGCGCCTT 
ATGGAGCACC TCAAACTCTT TTGGAGCAGA ATGAACCTCC CCAAGATGAT CCGTGCTTGC 
GAGGAAGCCA ACTTGTGGCC TGAGCTGGTC TTCTGCTACT ACCACTACGA TGAGTTTGAC 
AATGCTGCTC TCGCCGTTAT GGAGCGCCCT GAGAACTCTT GGGAGCACCA GCAGTTCAAG 
GAGATCACCG TCAAGGTGGC CAACCTTGAA ATCTACTACA AGGCCATCAA TTTCTACCTG 
GAGCAACATC CCTCGTTGCT CACCGATCTT CTCCAGGTTT TGACCCCGCG CATCGATGTC 
AACCGTGTTG TCCGCATGTT CCAGAAGTCC GATAACCTGC CCCTGATCAA GCCTTTCCTC 
CTTAGCGTCC AATCTCAGAA CAAGCGGACC GTTAACGACG CCATCAATGA TCTTCTCATT 
GAGGAGGAGG ACTACAAGAC TCTGCGCGAC TCTGTGAACA ACTACGACAA CTACGACGCC 
GTGGACCTTG CCGGTCGTCT GGAGAAGCAC GACCTTGTCT TCTTCCGCCA AATTGCTGCC 
AGCATCTACC GCAAGAACAA GCGCTGGGAG AAGTCCATCA ACCTTTCCAA GCAGGATAAG 
CTCTGGAAGG ATGCTATTGA GACGGCCGCC ATCTCCGGCA AGACGGAAGT TGTCGAGGAG 
CTTCTGCGCT ACTTCGTTGA CATTGGCAAC CGCGAATGCT ACGTTGGTAT GCTTTATGCC 
TGCTATGACC TCATCAGGCC CGACCTTGTC CTGGAGCTTT CTTGGCGCAA TGGTCTCAAT 
GATTTTACCA TGCCTTATAT GATCAACATG CTCTGCCAGC AGACCAAGGA GCTCGCTTCT 
CTGAAGGCCG ACAACGAGGC TCGCAAGGCT AAGGAGCAGG AGAAGGAGAA GGTCGAGGAC 
AACACGCCCA TTCTTGGCAA CCGGCTTATG ATCACTGCCG GCCCCGGCCA GGCATCCCCA 
GCTCCTTACG GACAAACCAA CGGTTTTGTG CCGCAGCCTA CCGGTTTTGG CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_965641.1
CDS	601..5637
Translation	MAQALPIKFQELLQLSALGINQTAITFNTCTLESDNWVCIRDKKDEAASPEVIIVDLKNGNNVIRRPIKADSAIMHWTRQVIALKAQARTLQIFDLENKAKLKSTNMSEDVLFWKWVSETTLGLITDGSVYHWDVFDPNQPAPVKVFDRNANLQGNQIINYRTSADGKWMVVVGISQQQGRVVGAMQLYSKDRGISQAIEGHAAAFGTLRLEGAPEDTKVFTFAVRTAVGAKLHIVEVDHPETNPVFPKKAVDVFFPPEASNDFPVALQVSQKYGIIYLITKYGFIHLYDLETATCIFMNRISSDTIFTACGDKDSTGVIGINRKGQVLFVSADENTIVPYVLEAHGNELAIKLASRAGLPGADNLYQQRFEQLFSNGNYQEAAKVAANSPRGFLRTPQTIERFKRLPQQPGQMSHILQYFGMLLDKGSLNEHETIELAQPVLAQNRKQLLQKWLSENKLECSERLGDMVRPHDINMALAIYLKANAPHKVVAGFAETGQFEKILPYCGQANYQPDFIQLLHHIVRVNAEKGAEFATTLANHEGGPLVDFEKVVDIFQSQGMIQQATAFLLDALKDNKPEHAHLQTRLLEMNLVNAPQVADAILGNDMFSHFDKAHIANLCEQAGLLQKALELYEDPASIKRVIVNIPGMPNYNPEWLIEYFKALSVEQSLDCLDAMMKHNIRQNLQTVVQVATKYAELLGAQQLIDLFEKYKTAEGLYYFLASVVNVTEDPEVVFKYIEAATKMGQIREVERICRDNSVYNPEKVKNFLKEAKLSEMLPLMVVCDRFNFVHDLVLYLYQHQQFKSIEIYVQQVNPSRTPGVIGGLLDVDCDESIIRNLLSTVNPASIPIDELVQEVETRNRLKLLLPFLEATLAAGNQQQAVYNALAKIYIDSNNNPEKFLKENDQYDTLTVGKYCEKRDPNLAYIAYRKGQNDLELVNITNENQMYKAQARYLLERADRELWMFVLSENNVHRRSVVDQVISTAVPESTDPAKVSEAVTCFLNADLPGELIELLEKIVLEPSPFSDNQNLQNLLIFTAAKADKARVMDYIHKLDNFAADEIANVCIEVGLHEEAFEVYKKIDNKEAAVNVLVEHVVSIDRAQAYAEEVDIPQVWSKVAKAQLDGLRVSDSIESYIKAEDPKNYEEVIEVAVAAGKNEELIKYLRMARKTLREPVIDTALAFCYARLDQLPELEEFLRATNVANVEESGDKAYAEGFFEAAKIFYTSISNWAKLATTLVHLSDYQAAVDCARKANNIKVWKEVHEACVNKKEFRLAQICGLNLIVDAEQLQALVKQYETNGYFDELISLLEQGLGLERAHMGMFTELGIALSKYHPERLMEHLKLFWSRMNLPKMIRACEEANLWPELVFCYYHYDEFDNAALAVMERPENSWEHQQFKEITVKVANLEIYYKAINFYLEQHPSLLTDLLQVLTPRIDVNRVVRMFQKSDNLPLIKPFLLSVQSQNKRTVNDAINDLLIEEEDYKTLRDSVNNYDNYDAVDLAGRLEKHDLVFFRQIAASIYRKNKRWEKSINLSKQDKLWKDAIETAAISGKTEVVEELLRYFVDIGNRECYVGMLYACYDLIRPDLVLELSWRNGLNDFTMPYMINMLCQQTKELASLKADNEARKAKEQEKEKVEDNTPILGNRLMITAGPGQASPAPYGQTNGFVPQPTGFGF

Target ORF information:

RefSeq Version	XM_960548.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A clathrin heavy chain (NCU02510), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_960548.2

ORF Insert Sequence:

ATGGCTCAGG CCCTACCCAT CAAATTCCAG GAGCTGCTGC AACTCAGCGC CCTCGGCATC 
AACCAGACTG CCATCACTTT CAATACCTGT ACTCTAGAAT CGGACAACTG GGTGTGTATT 
CGCGACAAGA AGGACGAGGC AGCGTCCCCT GAAGTCATCA TCGTCGATCT CAAGAACGGC 
AATAATGTTA TCCGACGTCC CATCAAGGCC GACAGTGCCA TAATGCACTG GACGCGCCAA 
GTAATTGCCC TCAAGGCCCA GGCGCGCACA CTCCAGATTT TCGACCTCGA AAACAAGGCC 
AAGCTCAAGT CGACAAACAT GAGCGAGGAT GTCTTGTTCT GGAAGTGGGT TAGCGAGACC 
ACGCTTGGCT TGATCACAGA TGGCTCTGTT TACCATTGGG ATGTCTTCGA CCCGAACCAG 
CCCGCTCCGG TCAAGGTCTT CGATCGTAAT GCTAATCTCC AGGGCAACCA AATAATTAAT 
TATCGGACCA GCGCCGACGG AAAGTGGATG GTTGTTGTCG GTATCTCACA ACAGCAGGGC 
CGCGTTGTTG GCGCCATGCA GCTCTATTCC AAAGACCGCG GTATCAGCCA GGCTATTGAG 
GGTCATGCCG CTGCTTTTGG CACCCTCCGA CTAGAGGGCG CCCCGGAGGA CACCAAGGTG 
TTTACCTTTG CGGTCCGCAC CGCCGTCGGC GCGAAGCTCC ACATTGTCGA GGTCGACCAT 
CCCGAAACAA ACCCCGTCTT CCCCAAGAAG GCCGTGGACG TCTTCTTCCC TCCCGAGGCC 
AGCAATGACT TCCCCGTCGC CCTCCAGGTT TCGCAAAAGT ACGGCATCAT CTACCTTATC 
ACCAAGTACG GTTTCATCCA CCTCTACGAC CTCGAGACTG CCACATGTAT CTTCATGAAC 
CGCATCTCAA GCGATACTAT CTTCACTGCT TGCGGTGACA AGGATTCGAC CGGCGTCATT 
GGCATCAACC GCAAGGGACA GGTTCTCTTC GTCAGCGCCG ATGAGAACAC AATCGTTCCT 
TACGTCCTTG AGGCACACGG CAATGAGCTC GCTATCAAGT TGGCTTCTCG TGCAGGGCTC 
CCAGGCGCCG ATAACCTCTA CCAGCAACGC TTCGAGCAAC TCTTCAGCAA TGGCAACTAC 
CAAGAGGCCG CCAAGGTGGC TGCCAACTCT CCTCGAGGTT TCCTTCGCAC TCCTCAGACC 
ATCGAGCGCT TCAAGCGTCT TCCACAGCAG CCTGGACAGA TGTCGCATAT CTTGCAGTAC 
TTTGGTATGC TTCTAGACAA GGGTTCGCTG AACGAGCATG AGACCATTGA GCTTGCCCAA 
CCCGTTCTCG CTCAGAACCG CAAACAGCTA TTGCAGAAGT GGCTCAGCGA GAACAAGCTC 
GAGTGCTCTG AGAGACTTGG TGACATGGTC CGGCCCCACG ACATTAACAT GGCGCTTGCC 
ATCTATTTGA AGGCCAACGC TCCTCACAAG GTTGTTGCTG GGTTTGCCGA GACTGGCCAG 
TTTGAGAAGA TTCTGCCCTA CTGCGGTCAA GCCAACTACC AGCCTGACTT TATTCAGCTT 
CTCCACCACA TTGTCCGTGT CAATGCTGAG AAGGGTGCCG AGTTTGCCAC GACGCTTGCC 
AATCACGAAG GCGGCCCGCT GGTCGACTTC GAGAAGGTGG TTGACATCTT CCAGTCTCAA 
GGAATGATCC AGCAAGCAAC TGCTTTCCTT CTTGACGCTC TGAAGGATAA CAAGCCCGAG 
CATGCCCACC TTCAGACTCG TCTATTGGAG ATGAACCTCG TCAACGCCCC CCAGGTTGCT 
GACGCCATTC TTGGCAACGA CATGTTCTCC CACTTTGACA AGGCGCACAT TGCGAACCTC 
TGCGAGCAAG CAGGTCTTTT GCAGAAGGCC CTGGAGCTCT ATGAGGATCC TGCGTCCATC 
AAGCGGGTTA TTGTCAACAT CCCTGGAATG CCTAACTACA ACCCCGAGTG GTTGATTGAG 
TATTTCAAGG CGCTGTCTGT TGAGCAATCG CTTGACTGCC TGGACGCCAT GATGAAGCAC 
AATATTCGCC AGAATCTCCA GACTGTCGTT CAAGTCGCAA CCAAGTATGC GGAGCTCCTC 
GGCGCGCAGC AATTGATTGA TCTCTTTGAG AAGTACAAGA CTGCTGAAGG TCTTTACTAC 
TTCCTTGCCA GTGTCGTCAA TGTCACCGAG GACCCCGAAG TCGTATTCAA GTACATTGAG 
GCTGCCACCA AGATGGGACA AATTCGCGAG GTTGAGCGTA TCTGCCGCGA CAACAGCGTT 
TACAACCCCG AGAAGGTGAA GAACTTCCTC AAGGAGGCCA AGCTCAGCGA GATGCTTCCT 
TTGATGGTTG TCTGCGATCG CTTCAACTTT GTTCATGACC TGGTTCTCTA CCTGTACCAG 
CACCAGCAGT TCAAGTCCAT TGAGATTTAC GTGCAGCAGG TCAACCCGAG CAGGACTCCT 
GGTGTCATCG GAGGACTGCT CGATGTGGAC TGTGATGAAA GCATCATCAG GAATCTCCTA 
AGCACGGTCA ACCCCGCATC TATCCCTATC GATGAGCTGG TGCAGGAGGT TGAGACTCGT 
AACCGCCTTA AGCTACTCCT GCCCTTCCTG GAGGCTACCC TCGCTGCCGG TAACCAGCAG 
CAGGCTGTTT ACAACGCTCT CGCCAAGATA TACATTGACT CCAACAACAA CCCAGAGAAG 
TTCCTCAAGG AGAACGATCA GTATGATACC CTCACTGTTG GAAAGTACTG CGAGAAGCGT 
GATCCCAACT TGGCCTATAT TGCCTATCGC AAGGGCCAGA ATGATCTCGA ACTCGTGAAC 
ATCACCAACG AGAACCAGAT GTACAAGGCT CAGGCGAGGT ATCTTTTGGA GCGCGCTGAT 
AGGGAACTTT GGATGTTTGT GCTCAGCGAG AACAACGTTC ACCGCCGTTC TGTCGTAGAC 
CAAGTCATCT CGACGGCCGT TCCTGAGTCG ACTGATCCCG CCAAGGTTTC CGAGGCTGTT 
ACGTGCTTCC TGAACGCCGA CTTGCCTGGC GAGCTTATTG AGCTGCTGGA GAAGATTGTC 
CTGGAGCCGT CGCCTTTCAG CGACAACCAG AACCTGCAGA ACTTGCTTAT CTTCACTGCC 
GCCAAGGCTG ACAAGGCCCG TGTGATGGAC TACATTCACA AGCTCGACAA CTTCGCCGCC 
GACGAGATTG CTAATGTTTG TATTGAGGTT GGCCTGCACG AGGAAGCCTT TGAGGTCTAC 
AAGAAGATTG ACAACAAGGA GGCCGCTGTC AATGTTCTTG TTGAGCACGT CGTTAGCATT 
GACCGTGCTC AAGCATACGC CGAGGAGGTT GATATTCCTC AGGTTTGGAG CAAAGTTGCC 
AAGGCTCAGC TTGATGGTCT CCGCGTCAGC GATTCCATTG AATCTTACAT CAAGGCCGAG 
GATCCGAAGA ACTACGAGGA AGTGATCGAG GTTGCCGTTG CTGCTGGAAA GAACGAAGAG 
CTCATCAAGT ACCTCCGTAT GGCCCGCAAG ACCCTTCGGG AGCCTGTCAT TGACACTGCT 
CTTGCTTTCT GCTACGCCCG CCTCGATCAA CTTCCTGAGC TTGAAGAGTT CCTGCGTGCT 
ACCAATGTTG CCAATGTTGA GGAGTCTGGT GACAAGGCGT ACGCCGAGGG CTTCTTTGAG 
GCGGCCAAAA TCTTCTACAC CAGCATTTCC AACTGGGCTA AGCTGGCCAC TACTCTTGTC 
CACCTGTCCG ACTACCAGGC GGCTGTGGAC TGCGCCCGCA AGGCCAACAA CATCAAGGTG 
TGGAAGGAGG TCCACGAGGC CTGCGTCAAC AAGAAGGAGT TCCGTCTTGC TCAGATTTGC 
GGTCTCAACC TTATTGTTGA TGCTGAGCAA CTCCAGGCTC TTGTCAAGCA GTACGAGACA 
AACGGCTACT TTGATGAGCT CATCAGCTTG CTCGAGCAGG GTCTTGGATT GGAGCGCGCC 
CACATGGGAA TGTTCACCGA GTTGGGTATT GCGCTGTCTA AATACCACCC CGAGCGCCTT 
ATGGAGCACC TCAAACTCTT TTGGAGCAGA ATGAACCTCC CCAAGATGAT CCGTGCTTGC 
GAGGAAGCCA ACTTGTGGCC TGAGCTGGTC TTCTGCTACT ACCACTACGA TGAGTTTGAC 
AATGCTGCTC TCGCCGTTAT GGAGCGCCCT GAGAACTCTT GGGAGCACCA GCAGTTCAAG 
GAGATCACCG TCAAGGTGGC CAACCTTGAA ATCTACTACA AGGCCATCAA TTTCTACCTG 
GAGCAACATC CCTCGTTGCT CACCGATCTT CTCCAGGTTT TGACCCCGCG CATCGATGTC 
AACCGTGTTG TCCGCATGTT CCAGAAGTCC GATAACCTGC CCCTGATCAA GCCTTTCCTC 
CTTAGCGTCC AATCTCAGAA CAAGCGGACC GTTAACGACG CCATCAATGA TCTTCTCATT 
GAGGAGGAGG ACTACAAGAC TCTGCGCGAC TCTGTGAACA ACTACGACAA CTACGACGCC 
GTGGACCTTG CCGGTCGTCT GGAGAAGCAC GACCTTGTCT TCTTCCGCCA AATTGCTGCC 
AGCATCTACC GCAAGAACAA GCGCTGGGAG AAGTCCATCA ACCTTTCCAA GCAGGATAAG 
CTCTGGAAGG ATGCTATTGA GACGGCCGCC ATCTCCGGCA AGACGGAAGT TGTCGAGGAG 
CTTCTGCGCT ACTTCGTTGA CATTGGCAAC CGCGAATGCT ACGTTGGTAT GCTTTATGCC 
TGCTATGACC TCATCAGGCC CGACCTTGTC CTGGAGCTTT CTTGGCGCAA TGGTCTCAAT 
GATTTTACCA TGCCTTATAT GATCAACATG CTCTGCCAGC AGACCAAGGA GCTCGCTTCT 
CTGAAGGCCG ACAACGAGGC TCGCAAGGCT AAGGAGCAGG AGAAGGAGAA GGTCGAGGAC 
AACACGCCCA TTCTTGGCAA CCGGCTTATG ATCACTGCCG GCCCCGGCCA GGCATCCCCA 
GCTCCTTACG GACAAACCAA CGGTTTTGTG CCGCAGCCTA CCGGTTTTGG CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU02510 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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