AT3G08530 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT3G08530
Entrez Gene ID	820001
Full Name	Clathrin, heavy chain
Synonyms	AtCHC2,CHC2,clathrin heavy chain 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_111688.7	NP_187466.4	Clathrin, heavy chain

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cltca	NP_001005391.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003021	XP_311856.3
Magnaporthe oryzae (rice blast fungus)	MGG_07768	XP_003712981.1
Homo sapiens (human)	CLTC	NP_001275582.1
Bos taurus (cattle)	CLTC	NP_776448.1
Mus musculus (house mouse)	Cltc	NP_001003908.1
Schizosaccharomyces pombe (fission yeast)	chc1	NP_594148.1
Arabidopsis thaliana (thale cress)	AT3G08530	NP_187466.4
Kluyveromyces lactis	KLLA0F09911g	XP_455531.1
Eremothecium gossypii	AGOS_AER359W	NP_985215.2
Xenopus tropicalis (tropical clawed frog)	cltc	NP_001011039.1
Pan troglodytes (chimpanzee)	CLTC	XP_001136053.1
	CLTC	XP_001108647.2
Gallus gallus (chicken)	CLTC	NP_001073586.1
Danio rerio (zebrafish)	cltcb	NP_001181907.1
Drosophila melanogaster (fruit fly)	Chc	NP_996451.1
Saccharomyces cerevisiae (baker's yeast)	CHC1	NP_011309.1
Arabidopsis thaliana (thale cress)	AT3G11130	NP_187724.2
Canis lupus familiaris (dog)	CLTC	XP_537700.2
Rattus norvegicus (Norway rat)	Cltc	NP_062172.1
Caenorhabditis elegans (roundworm)	chc-1	NP_499260.1
Neurospora crassa	NCU02510	XP_965641.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EDR4 associates with CHC2 and modulates plant immunity by regulating the relocation of EDR1 in Arabidopsis.[CHC2]
Title: ENHANCED DISEASE RESISTANCE4 associates with CLATHRIN HEAVY CHAIN2 and modulates plant immunity by regulating relocation of EDR1 in Arabidopsis.

study presents a genetic characterization of the role of clathrin in endocytosis and auxin-mediated plant development; data demonstrate a fundamental role for clathrin function in cell polarity, growth, patterning, and organogenesis
Title: Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis.

The following AT3G08530 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT3G08530 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb10568	NM_111688.7 Latest version!	Arabidopsis thaliana Clathrin, heavy chain (AT3G08530), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT3G08530 ( NM_111688.7 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_187466.4 Arabidopsis thaliana Clathrin, heavy chain (AT3G08530), mRNA.

Price & Availability↑

CloneID	OAb10568
Clone ID Related Accession (Same CDS sequence)	NM_111688.7
Accession Version	NM_111688.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Clathrin, heavy chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_111688.6.

ATGGCGGCTG CCAACGCCCC CATCACGATG AAGGAGGTCC TGACGCTTCC GAGCATCGGG 
ATCAATCAGC AATTCATCAC GTTTACAAAT GTTACTATGG AGTCTGACAA GTATATCTGT 
GTACGAGAGA CGTCACCGCA AAACAGTGTT GTAATTATAG ATATGAATAT GCCGATGCAG 
CCTCTTAGGA GGCCTATTAC TGCAGACTCT GCTCTTATGA ACCCAAACTC GAAGATCCTT 
GCCTTGAAAG CTCAAGTTCC AGGAACTACT CAGGATCATC TACAGATATT TAACATTGAA 
GCCAAAGCAA AGTTGAAATC ACATCAGATG CCTGAGCAGG TTGTCTTTTG GAAGTGGATT 
ACACCAAAGA TGTTGGGGCT GGTCACACAG AATTCCGTCT ATCATTGGTC AATTGAAGGT 
GATTCTGAGC CAGTTAAGAT GTTTGATAGA ACAGCCAATT TGGCCAACAA TCAAATTATA 
AACTATAAGT GCTCTCCTAA TGAGAAGTGG TTGGTTTTAA TTGGAATTGC TCCTGGCTCT 
CCTGAGAGAC AACAATTGGT AAAAGGAAAT ATGCAGCTTT TCTCTGTGGA TCAACAGCGG 
AGTCAGGCCC TTGAGGCACA TGCTGCATCG TTTGCGCAGT TTAAGGTTCC TGGGAATGAG 
AATCCTTCTA TTCTTATATC ATTTGCAAGC AAGAGCTTTA ATGCTGGGCA GATAACATCA 
AAGTTGCATG TCATTGAGCT TGGTGCCCAA CCTGGAAAAC CATCATTTAC AAAAAAACAA 
GCAGATCTCT TTTTTCCCCC TGATTTTGCT GATGATTTTC CTGTTGCCAT GCAGGTCTCT 
CACAAATTTA ACTTGATATA TGTTATCACC AAACTTGGCC TGTTGTTTGT GTACGATCTA 
GAGACTGCTT CTGCTATCTA CAGAAATCGG ATTAGTCCAG ACCCAATTTT CTTGACTTCT 
GAAGCTTCCT CAGTTGGGGG TTTCTATGCC ATTAATAGGC GAGGACAAGT TCTTTTGGCT 
ACAGTAAACG AGGCTACCAT TATACCTTTT ATTAGTGGCC AATTGAACAA TTTGGAGCTT 
GCTGTCAATC TTGCTAAAAG AGGAAACCTC CCAGGTGCAG AAAATCTGGT TGTCCAGCGG 
TTCCAGGAGT TGTTCGCTCA AACAAAGTAC AAGGAGGCTG CTGAGCTTGC TGCTGAATCT 
CCTCAAGGAA TTCTAAGGAC ACCTGATACA GTGGCCAAAT TCCAGAGTGT TCCTGTACAA 
GCTGGACAAA CTCCTCCATT GTTACAGTAT TTTGGGACTC TTTTGACTAG AGGGAAACTC 
AATTCATATG AGTCACTGGA ACTATCTCGG CTGGTTGTGA ACCAAAACAA GAAGAACCTC 
CTGGAAAACT GGTTGGCAGA GGATAAACTA GAATGCAGTG AAGAACTTGG AGACCTTGTG 
AAGACTGTGG ATAATGATCT CGCTCTGAAA ATATACATCA AAGCTCGGGC GACTCCAAAA 
GTTGTAGCAG CTTTTGCAGA AAGAAGGGAG TTTGACAAAA TACTGATTTA CTCAAAGCAG 
GTTGGGTACA CACCTGATTA CCTGTTTCTT CTGCAAACCA TACTCCGTAC AGATCCTCAG 
GGAGCAGTAA ATTTTGCATT AATGATGTCT CAAATGGAAG GAGGTTCTCC AGTTGACTAC 
AACACCATCA CTGATCTTTT TCTTCAGAGA AATCTGATCC GTGAAGCGAC TTCTTTCCTT 
CTGGATGTGC TAAAGCCAAA TTTACCTGAG CATGCTTTTC TGCAAACTAA GGTTCTGGAG 
ATAAATTTGG TAACTTTTCC TAATGTGGCT GATGCGGTAT TAGCAAATGG GATGTTCACC 
CACTATGACC GGCCTCGCAT TGCTCAACTT TGTGAAAAGG CTGGACTTTA TATCCAATCC 
TTGAAGCATT ACTCAGAGTT ACCTGATATA AAACGTGTAA TTGTAAACAC ACATGCTATC 
GAGCCACAGG CTCTTGTTGA GTTTTTTGGC ACTCTTTCTA GCGAGTGGGC TATGGAGTGC 
ATGAAGGATC TGTTGCTGGT CAACCTGAGA GGCAATCTGC AGATAATTGT TCAGGCTTGC 
AAGGAGTACT GTGAGCAACT TGGTGTTGAT GCATGTATTA AGCTCTTTGA GCAGTTCAAG 
TCATATGAAG GGCTATACTT TTTCCTAGGT TCATATTTGA GTATGAGTGA GGATCCTGAG 
ATCCATTTCA AGTACATTGA AGCAGCTGCC AAGACTGGTC AAATAAAGGA GGTTGAGCGT 
GTAACTAGAG AGTCTAACTT CTATGATGCT GAAAAGACGA AGAACTTTTT GATGGAAGCT 
AAGCTGCCTG ATGCACGACC TTTGATAAAT GTCTGTGATC GTTTCAGCTT TGTGCCTGAT 
CTTACTCATT ACCTCTACAC AAACAACATG CTTCGTTACA TTGAAGGCTA CGTGCAGAAG 
GTGAACCCTG GGAATGCTCC CTTAGTTGTC GGGCAGTTGC TTGATGATGA ATGCCCTGAA 
GATTTTATAA AAGGCCTCAT TCTCTCTGTT CGTTCATTGC TTCCTGTTGA ACCACTTGTC 
GAGGAATGTG AGAAGAGAAA TCGACTCCGT CTCCTCACTC AGTTCTTGGA GCATTTAGTT 
AGTGAGGGAA GCCAAGATGT GCATGTCCAT AATGCCTTGG GTAAAATTAT CATAGACAGT 
AACAACAACC CTGAGCATTT CCTGACCACC AACCCTTACT ACGACTCTAA GGTTGTGGGT 
AAGTACTGCG AGAAACGTGA TCCTACCCTA GCAGTTGTGG CATACAGAAG AGGACAATGT 
GACGAGGAAC TCATCAATGT CACTAACAAA AACTCTTTGT TTAAGTTACA AGCCAGGTAT 
GTAGTGGAGA GGATGGATGG TGATCTGTGG GATAAGGTTC TCGATGAAAA CAATGATTAT 
AGAAGACAGC TTATTGACCA AGTTGTGTCT ACTGCTTTAC CTGAAAGCAA GAGCCCAGAG 
CAAGTTTCTG CAGCTGTTAA AGCATTCATG ACTGCTGATT TGCCGCACGA GCTTATTGAG 
CTTCTTGAAA AGATTGTTCT ACAAAATTCT GCCTTCAGTG GAAACTTCAA TCTGCAAAAT 
CTGCTTATAT TGACAGCTAT TAAGGCAGAT CCGTCTAGAG TTATGGATTA CATCAACAGG 
CTGGACAACT TTGATGGACC TGCAGTTGGA GAAGTCGCAG TTGAAGCCCA ATTATATGAG 
GAAGCATTTG CTATCTTCAA GAAGTTCAAC TTAAATGTTC AGGCTGTCAA TGTATTGCTG 
GATAATGTCA GGAGCATTGA GCGTGCAGTT GAGTTTGCAT TCCGGGTCGA AGAAGATTCT 
GTTTGGAGCC AAGTCGCTAA GGCGCAACTC AGGGAAGGAC TAGTCAGTGA TGCAATTGAG 
TCCTTTATCC GTGCTGATGA TGCCACTCAT TTCTTGGAGG TCATACGTGT CTCTGAAGAT 
ACTGATGTCT ATGATGACTT GGTCAAATAC CTTCTGATGG TTAGACAGAA GGTGAAGGAA 
CCCAAGGTTG ATAGTGAGCT CATCTATGCA TATGCAAAGA TTGATCGGTT AGGTGAGATT 
GAAGAATTCA TTCTGATGCC AAACGTTGCT AATCTACAAC ATGTTGGTGA TCGTCTGTAT 
GACGAAGCTC TCTATGAGGC TGCAAAAATA ATCTATGCGT TCATATCAAA CTGGGGCAAG 
TTAGCCGTCA CACTTGTGAA GTTGCAACAG TTCCAAGGTG CTGTTGATGC TGCAAGGAAA 
GCAAACAGTG CAAAGACATG GAAGGAAGTC TGCTTTGCTT GCGTTGATGC TGAGGAATTC 
CGGTTGGCTC AAATATGTGG TCTTAACATT ATCATTCAGG TGGATGACCT GGAAGAAGTC 
AGCGAGTACT ACCAGAACAG AGGGTGCTTC AATGAATTAA TCTCACTTAT GGAGAGTGGA 
CTTGGGCTTG AACGTGCTCA CATGGGTATC TTCACCGAGT TGGGTGTACT CTATGCTAGA 
TATCGTTATG AGAAACTTAT GGAACACATC AAGTTATTTT CAACTCGCCT CAATATTCCC 
AAGCTTATAC GGGCCTGTGA TGAACAACAG CACTGGCAGG AACTTACCTA TCTGTACATT 
CAATATGATG AGTTTGATAA TGCTGCGACC ACCGTCATGA ACCATTCTCC TGAAGCATGG 
GAGCACATGC AATTCAAAGA CATTGTTGCC AAGGTAGCGA ATGTTGAACT GTACTACAAG 
GCTGTGCACT TCTACTTGCA AGAGCATCCG GATATAATCA ACGATCTTCT CAACGTACTT 
GCGTTGCGGT TAGACCATAC TCGTGTTGTT GATATAATGC GGAAGGCTGG TCACTTGCGT 
CTTATCAAGC CATACATGAT TGCAGTTCAG AGCAACAATG TCTCTGCTGT TAATGAGGCT 
CTGAATGAGA TATACGTGGA AGAAGAAGAC TACGATAGGT TGCGAGAGTC TATTGATTTA 
CATGACAGCT TTGACCAGAT TGGCCTCGCT CAGAAGATCG AGAAACACGA ACTTGTTGAA 
ATGAGACGTG TGGCTGCTTA TATCTACAAG AAAGCGGGCA GATGGAAGCA ATCAATTGCA 
TTATCAAAGA AAGACAACAT GTACAAGGAC TGTATGGAAA CGGCTTCACA ATCAGGCGAA 
CATGAACTTG CAGAACAACT ACTGGTGTAT TTCATTGAAC AGGGCAAAAA GGAATGCTTT 
GCTACATGTC TCTTTGTATG TTATGACTTA ATCCGACCAG ACGTGGCTCT AGAACTTGCC 
TGGATCAACA ATATGATGGA CTTTGCCTTC CCGTATCTCC TCCAGTTTAT CCGAGAGTAC 
TCTGGCAAAG TGGATGAGCT AATAAAGGAC AAGCTTGAGG CACAAAAAGA GGTGAAGGCC 
AAAGAGCAGG AAGAGAAGGA TGTCATATCC CAACAGAACA TGTATGCCCA AATGTTGCCA 
CTTGCTTTAC CTGCACCACC GATGCCAGGA ATGGGAGGTG GTGGTGGGTA TGGTCCTCCA 
CCACAAATGG GCGGTATGCC AGGAATGCCT CCCATGCCAC CATATGGAAT GCCACCGATG 
GGCGGCTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_187466.4
CDS	244..5355
Translation	MAAANAPITMKEVLTLPSIGINQQFITFTNVTMESDKYICVRETSPQNSVVIIDMNMPMQPLRRPITADSALMNPNSKILALKAQVPGTTQDHLQIFNIEAKAKLKSHQMPEQVVFWKWITPKMLGLVTQNSVYHWSIEGDSEPVKMFDRTANLANNQIINYKCSPNEKWLVLIGIAPGSPERQQLVKGNMQLFSVDQQRSQALEAHAASFAQFKVPGNENPSILISFASKSFNAGQITSKLHVIELGAQPGKPSFTKKQADLFFPPDFADDFPVAMQVSHKFNLIYVITKLGLLFVYDLETASAIYRNRISPDPIFLTSEASSVGGFYAINRRGQVLLATVNEATIIPFISGQLNNLELAVNLAKRGNLPGAENLVVQRFQELFAQTKYKEAAELAAESPQGILRTPDTVAKFQSVPVQAGQTPPLLQYFGTLLTRGKLNSYESLELSRLVVNQNKKNLLENWLAEDKLECSEELGDLVKTVDNDLALKIYIKARATPKVVAAFAERREFDKILIYSKQVGYTPDYLFLLQTILRTDPQGAVNFALMMSQMEGGSPVDYNTITDLFLQRNLIREATSFLLDVLKPNLPEHAFLQTKVLEINLVTFPNVADAVLANGMFTHYDRPRIAQLCEKAGLYIQSLKHYSELPDIKRVIVNTHAIEPQALVEFFGTLSSEWAMECMKDLLLVNLRGNLQIIVQACKEYCEQLGVDACIKLFEQFKSYEGLYFFLGSYLSMSEDPEIHFKYIEAAAKTGQIKEVERVTRESNFYDAEKTKNFLMEAKLPDARPLINVCDRFSFVPDLTHYLYTNNMLRYIEGYVQKVNPGNAPLVVGQLLDDECPEDFIKGLILSVRSLLPVEPLVEECEKRNRLRLLTQFLEHLVSEGSQDVHVHNALGKIIIDSNNNPEHFLTTNPYYDSKVVGKYCEKRDPTLAVVAYRRGQCDEELINVTNKNSLFKLQARYVVERMDGDLWDKVLDENNDYRRQLIDQVVSTALPESKSPEQVSAAVKAFMTADLPHELIELLEKIVLQNSAFSGNFNLQNLLILTAIKADPSRVMDYINRLDNFDGPAVGEVAVEAQLYEEAFAIFKKFNLNVQAVNVLLDNVRSIERAVEFAFRVEEDSVWSQVAKAQLREGLVSDAIESFIRADDATHFLEVIRVSEDTDVYDDLVKYLLMVRQKVKEPKVDSELIYAYAKIDRLGEIEEFILMPNVANLQHVGDRLYDEALYEAAKIIYAFISNWGKLAVTLVKLQQFQGAVDAARKANSAKTWKEVCFACVDAEEFRLAQICGLNIIIQVDDLEEVSEYYQNRGCFNELISLMESGLGLERAHMGIFTELGVLYARYRYEKLMEHIKLFSTRLNIPKLIRACDEQQHWQELTYLYIQYDEFDNAATTVMNHSPEAWEHMQFKDIVAKVANVELYYKAVHFYLQEHPDIINDLLNVLALRLDHTRVVDIMRKAGHLRLIKPYMIAVQSNNVSAVNEALNEIYVEEEDYDRLRESIDLHDSFDQIGLAQKIEKHELVEMRRVAAYIYKKAGRWKQSIALSKKDNMYKDCMETASQSGEHELAEQLLVYFIEQGKKECFATCLFVCYDLIRPDVALELAWINNMMDFAFPYLLQFIREYSGKVDELIKDKLEAQKEVKAKEQEEKDVISQQNMYAQMLPLALPAPPMPGMGGGGGYGPPPQMGGMPGMPPMPPYGMPPMGGY

Target ORF information:

RefSeq Version	NM_111688.7
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Clathrin, heavy chain (AT3G08530), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_111688.7

ORF Insert Sequence:

ATGGCGGCTG CCAACGCCCC CATCACGATG AAGGAGGTCC TGACGCTTCC GAGCATCGGG 
ATCAATCAGC AATTCATCAC GTTTACAAAT GTTACTATGG AGTCTGACAA GTATATCTGT 
GTACGAGAGA CGTCACCGCA AAACAGTGTT GTAATTATAG ATATGAATAT GCCGATGCAG 
CCTCTTAGGA GGCCTATTAC TGCAGACTCT GCTCTTATGA ACCCAAACTC GAAGATCCTT 
GCCTTGAAAG CTCAAGTTCC AGGAACTACT CAGGATCATC TACAGATATT TAACATTGAA 
GCCAAAGCAA AGTTGAAATC ACATCAGATG CCTGAGCAGG TTGTCTTTTG GAAGTGGATT 
ACACCAAAGA TGTTGGGGCT GGTCACACAG AATTCCGTCT ATCATTGGTC AATTGAAGGT 
GATTCTGAGC CAGTTAAGAT GTTTGATAGA ACAGCCAATT TGGCCAACAA TCAAATTATA 
AACTATAAGT GCTCTCCTAA TGAGAAGTGG TTGGTTTTAA TTGGAATTGC TCCTGGCTCT 
CCTGAGAGAC AACAATTGGT AAAAGGAAAT ATGCAGCTTT TCTCTGTGGA TCAACAGCGG 
AGTCAGGCCC TTGAGGCACA TGCTGCATCG TTTGCGCAGT TTAAGGTTCC TGGGAATGAG 
AATCCTTCTA TTCTTATATC ATTTGCAAGC AAGAGCTTTA ATGCTGGGCA GATAACATCA 
AAGTTGCATG TCATTGAGCT TGGTGCCCAA CCTGGAAAAC CATCATTTAC AAAAAAACAA 
GCAGATCTCT TTTTTCCCCC TGATTTTGCT GATGATTTTC CTGTTGCCAT GCAGGTCTCT 
CACAAATTTA ACTTGATATA TGTTATCACC AAACTTGGCC TGTTGTTTGT GTACGATCTA 
GAGACTGCTT CTGCTATCTA CAGAAATCGG ATTAGTCCAG ACCCAATTTT CTTGACTTCT 
GAAGCTTCCT CAGTTGGGGG TTTCTATGCC ATTAATAGGC GAGGACAAGT TCTTTTGGCT 
ACAGTAAACG AGGCTACCAT TATACCTTTT ATTAGTGGCC AATTGAACAA TTTGGAGCTT 
GCTGTCAATC TTGCTAAAAG AGGAAACCTC CCAGGTGCAG AAAATCTGGT TGTCCAGCGG 
TTCCAGGAGT TGTTCGCTCA AACAAAGTAC AAGGAGGCTG CTGAGCTTGC TGCTGAATCT 
CCTCAAGGAA TTCTAAGGAC ACCTGATACA GTGGCCAAAT TCCAGAGTGT TCCTGTACAA 
GCTGGACAAA CTCCTCCATT GTTACAGTAT TTTGGGACTC TTTTGACTAG AGGGAAACTC 
AATTCATATG AGTCACTGGA ACTATCTCGG CTGGTTGTGA ACCAAAACAA GAAGAACCTC 
CTGGAAAACT GGTTGGCAGA GGATAAACTA GAATGCAGTG AAGAACTTGG AGACCTTGTG 
AAGACTGTGG ATAATGATCT CGCTCTGAAA ATATACATCA AAGCTCGGGC GACTCCAAAA 
GTTGTAGCAG CTTTTGCAGA AAGAAGGGAG TTTGACAAAA TACTGATTTA CTCAAAGCAG 
GTTGGGTACA CACCTGATTA CCTGTTTCTT CTGCAAACCA TACTCCGTAC AGATCCTCAG 
GGAGCAGTAA ATTTTGCATT AATGATGTCT CAAATGGAAG GAGGTTCTCC AGTTGACTAC 
AACACCATCA CTGATCTTTT TCTTCAGAGA AATCTGATCC GTGAAGCGAC TTCTTTCCTT 
CTGGATGTGC TAAAGCCAAA TTTACCTGAG CATGCTTTTC TGCAAACTAA GGTTCTGGAG 
ATAAATTTGG TAACTTTTCC TAATGTGGCT GATGCGGTAT TAGCAAATGG GATGTTCACC 
CACTATGACC GGCCTCGCAT TGCTCAACTT TGTGAAAAGG CTGGACTTTA TATCCAATCC 
TTGAAGCATT ACTCAGAGTT ACCTGATATA AAACGTGTAA TTGTAAACAC ACATGCTATC 
GAGCCACAGG CTCTTGTTGA GTTTTTTGGC ACTCTTTCTA GCGAGTGGGC TATGGAGTGC 
ATGAAGGATC TGTTGCTGGT CAACCTGAGA GGCAATCTGC AGATAATTGT TCAGGCTTGC 
AAGGAGTACT GTGAGCAACT TGGTGTTGAT GCATGTATTA AGCTCTTTGA GCAGTTCAAG 
TCATATGAAG GGCTATACTT TTTCCTAGGT TCATATTTGA GTATGAGTGA GGATCCTGAG 
ATCCATTTCA AGTACATTGA AGCAGCTGCC AAGACTGGTC AAATAAAGGA GGTTGAGCGT 
GTAACTAGAG AGTCTAACTT CTATGATGCT GAAAAGACGA AGAACTTTTT GATGGAAGCT 
AAGCTGCCTG ATGCACGACC TTTGATAAAT GTCTGTGATC GTTTCAGCTT TGTGCCTGAT 
CTTACTCATT ACCTCTACAC AAACAACATG CTTCGTTACA TTGAAGGCTA CGTGCAGAAG 
GTGAACCCTG GGAATGCTCC CTTAGTTGTC GGGCAGTTGC TTGATGATGA ATGCCCTGAA 
GATTTTATAA AAGGCCTCAT TCTCTCTGTT CGTTCATTGC TTCCTGTTGA ACCACTTGTC 
GAGGAATGTG AGAAGAGAAA TCGACTCCGT CTCCTCACTC AGTTCTTGGA GCATTTAGTT 
AGTGAGGGAA GCCAAGATGT GCATGTCCAT AATGCCTTGG GTAAAATTAT CATAGACAGT 
AACAACAACC CTGAGCATTT CCTGACCACC AACCCTTACT ACGACTCTAA GGTTGTGGGT 
AAGTACTGCG AGAAACGTGA TCCTACCCTA GCAGTTGTGG CATACAGAAG AGGACAATGT 
GACGAGGAAC TCATCAATGT CACTAACAAA AACTCTTTGT TTAAGTTACA AGCCAGGTAT 
GTAGTGGAGA GGATGGATGG TGATCTGTGG GATAAGGTTC TCGATGAAAA CAATGATTAT 
AGAAGACAGC TTATTGACCA AGTTGTGTCT ACTGCTTTAC CTGAAAGCAA GAGCCCAGAG 
CAAGTTTCTG CAGCTGTTAA AGCATTCATG ACTGCTGATT TGCCGCACGA GCTTATTGAG 
CTTCTTGAAA AGATTGTTCT ACAAAATTCT GCCTTCAGTG GAAACTTCAA TCTGCAAAAT 
CTGCTTATAT TGACAGCTAT TAAGGCAGAT CCGTCTAGAG TTATGGATTA CATCAACAGG 
CTGGACAACT TTGATGGACC TGCAGTTGGA GAAGTCGCAG TTGAAGCCCA ATTATATGAG 
GAAGCATTTG CTATCTTCAA GAAGTTCAAC TTAAATGTTC AGGCTGTCAA TGTATTGCTG 
GATAATGTCA GGAGCATTGA GCGTGCAGTT GAGTTTGCAT TCCGGGTCGA AGAAGATTCT 
GTTTGGAGCC AAGTCGCTAA GGCGCAACTC AGGGAAGGAC TAGTCAGTGA TGCAATTGAG 
TCCTTTATCC GTGCTGATGA TGCCACTCAT TTCTTGGAGG TCATACGTGT CTCTGAAGAT 
ACTGATGTCT ATGATGACTT GGTCAAATAC CTTCTGATGG TTAGACAGAA GGTGAAGGAA 
CCCAAGGTTG ATAGTGAGCT CATCTATGCA TATGCAAAGA TTGATCGGTT AGGTGAGATT 
GAAGAATTCA TTCTGATGCC AAACGTTGCT AATCTACAAC ATGTTGGTGA TCGTCTGTAT 
GACGAAGCTC TCTATGAGGC TGCAAAAATA ATCTATGCGT TCATATCAAA CTGGGGCAAG 
TTAGCCGTCA CACTTGTGAA GTTGCAACAG TTCCAAGGTG CTGTTGATGC TGCAAGGAAA 
GCAAACAGTG CAAAGACATG GAAGGAAGTC TGCTTTGCTT GCGTTGATGC TGAGGAATTC 
CGGTTGGCTC AAATATGTGG TCTTAACATT ATCATTCAGG TGGATGACCT GGAAGAAGTC 
AGCGAGTACT ACCAGAACAG AGGGTGCTTC AATGAATTAA TCTCACTTAT GGAGAGTGGA 
CTTGGGCTTG AACGTGCTCA CATGGGTATC TTCACCGAGT TGGGTGTACT CTATGCTAGA 
TATCGTTATG AGAAACTTAT GGAACACATC AAGTTATTTT CAACTCGCCT CAATATTCCC 
AAGCTTATAC GGGCCTGTGA TGAACAACAG CACTGGCAGG AACTTACCTA TCTGTACATT 
CAATATGATG AGTTTGATAA TGCTGCGACC ACCGTCATGA ACCATTCTCC TGAAGCATGG 
GAGCACATGC AATTCAAAGA CATTGTTGCC AAGGTAGCGA ATGTTGAACT GTACTACAAG 
GCTGTGCACT TCTACTTGCA AGAGCATCCG GATATAATCA ACGATCTTCT CAACGTACTT 
GCGTTGCGGT TAGACCATAC TCGTGTTGTT GATATAATGC GGAAGGCTGG TCACTTGCGT 
CTTATCAAGC CATACATGAT TGCAGTTCAG AGCAACAATG TCTCTGCTGT TAATGAGGCT 
CTGAATGAGA TATACGTGGA AGAAGAAGAC TACGATAGGT TGCGAGAGTC TATTGATTTA 
CATGACAGCT TTGACCAGAT TGGCCTCGCT CAGAAGATCG AGAAACACGA ACTTGTTGAA 
ATGAGACGTG TGGCTGCTTA TATCTACAAG AAAGCGGGCA GATGGAAGCA ATCAATTGCA 
TTATCAAAGA AAGACAACAT GTACAAGGAC TGTATGGAAA CGGCTTCACA ATCAGGCGAA 
CATGAACTTG CAGAACAACT ACTGGTGTAT TTCATTGAAC AGGGCAAAAA GGAATGCTTT 
GCTACATGTC TCTTTGTATG TTATGACTTA ATCCGACCAG ACGTGGCTCT AGAACTTGCC 
TGGATCAACA ATATGATGGA CTTTGCCTTC CCGTATCTCC TCCAGTTTAT CCGAGAGTAC 
TCTGGCAAAG TGGATGAGCT AATAAAGGAC AAGCTTGAGG CACAAAAAGA GGTGAAGGCC 
AAAGAGCAGG AAGAGAAGGA TGTCATATCC CAACAGAACA TGTATGCCCA AATGTTGCCA 
CTTGCTTTAC CTGCACCACC GATGCCAGGA ATGGGAGGTG GTGGTGGGTA TGGTCCTCCA 
CCACAAATGG GCGGTATGCC AGGAATGCCT CCCATGCCAC CATATGGAAT GCCACCGATG 
GGCGGCTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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AT3G08530 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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