CHC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CHC1
Entrez Gene ID	852666
Full Name	clathrin heavy chain
Synonyms	SWA5
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181071.1	NP_011309.1	clathrin heavy chain

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cltca	NP_001005391.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003021	XP_311856.3
Magnaporthe oryzae (rice blast fungus)	MGG_07768	XP_003712981.1
Homo sapiens (human)	CLTC	NP_001275582.1
Bos taurus (cattle)	CLTC	NP_776448.1
Mus musculus (house mouse)	Cltc	NP_001003908.1
Schizosaccharomyces pombe (fission yeast)	chc1	NP_594148.1
Arabidopsis thaliana (thale cress)	AT3G08530	NP_187466.4
Kluyveromyces lactis	KLLA0F09911g	XP_455531.1
Eremothecium gossypii	AGOS_AER359W	NP_985215.2
Xenopus tropicalis (tropical clawed frog)	cltc	NP_001011039.1
Pan troglodytes (chimpanzee)	CLTC	XP_001136053.1
	CLTC	XP_001108647.2
Gallus gallus (chicken)	CLTC	NP_001073586.1
Danio rerio (zebrafish)	cltcb	NP_001181907.1
Drosophila melanogaster (fruit fly)	Chc	NP_996451.1
Saccharomyces cerevisiae (baker's yeast)	CHC1	NP_011309.1
Arabidopsis thaliana (thale cress)	AT3G11130	NP_187724.2
Canis lupus familiaris (dog)	CLTC	XP_537700.2
Rattus norvegicus (Norway rat)	Cltc	NP_062172.1
Caenorhabditis elegans (roundworm)	chc-1	NP_499260.1
Neurospora crassa	NCU02510	XP_965641.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that additional or alternative binding sites exist on the the clathrin heavy chain (HC) N-terminal domain propeller to help facilitate the recruitment of clathrin to sites of vesicle formation.
Title: Clathrin functions in the absence of the terminal domain binding site for adaptor-associated clathrin-box motifs.

The following CHC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02051	NM_001181071.1 Latest version!	Saccharomyces cerevisiae S288C clathrin heavy chain (CHC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHC1 ( NM_001181071.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011309.1 Saccharomyces cerevisiae S288C clathrin heavy chain (CHC1), partial mRNA.

Price & Availability↑

CloneID	OSi02051
Clone ID Related Accession (Same CDS sequence)	NM_001181071.1
Accession Version	NM_001181071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	clathrin heavy chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTGACC TACCCATTGA ATTTACCGAA TTGGTCGATC TGATGTCCTT AGGAATTTCC 
CCTCAATTCC TTGACTTCAG ATCAACTACT TTCGAGAGTG ACCACTTCGT CACTGTTAGA 
GAAACAAAGG ACGGTACAAA CTCTGTTGCA ATCGTGGATT TGGCCAAAGG CAATGAAGTG 
ACAAGGAAGA ATATGGGCGG TGATTCTGCT ATCATGCATC CATCTCAGAT GGTTATTTCC 
GTTAGAGCAA ATGGTACTAT CGTGCAGATA TTTAATTTGG AAACTAAGAG CAAGTTAAAG 
TCTTTTACTT TAGATGAACC TGTTATTTTT TGGAGGTGGT TGAGTGAAAC CACTTTGGGT 
TTTGTTACCG CAAGATCCAT ACTGACCTCG AACGTCTTTG ATGGTAACGT AAATGCTAAG 
CCTCAATTGC TTACCTTGAG ACACGCTAAC TTGAACAATA CCCAAATTAT CAATTTTGTG 
GCTAACAAAA ACCTTGACTG GTTCGCAGTT GTTGGTATCT TACAAGAAAA TGGCCGTATT 
GCCGGTAGAA TTCAATTATT CTCAAAACAA CGTAACATCT CCCAAGCTAT CGACGGTCAT 
GTTGCTATCT TTACGAATAT ATTACTAGAG GGTAATGGCT CGACCCCGGT ACAAGTTTTC 
GTTACTGGTA ACAGAAATGC CACCACCGGT GCTGGAGAAT TAAGAATTAT CGAGATTGAT 
CACGATGCTT CATTGCCTTC TCAATATCAA AAGGAAACTA CCGATATTTT CTTTCCACCA 
GATGCAACGA ATGATTTCCC AATCGCTGTT CAGGTGTCTG AGAAATACGG CATAATTTAC 
CTATTAACCA AGTATGGCTT CATCCATTTG TACGAATTGG AAACCGGTAC CAATTTATTC 
GTTAATAGAA TTACAGCAGA ATCTGTTTTT ACTGCAGCTC CGTACAATCA CGAGAACGGT 
ATTGCATGCA TCAATAAAAA AGGTCAAGTT TTAGCAGTAG AGATTTCTAC TTCTCAAATC 
GTTCCATATA TCTTGAATAA ATTATCTAAT GTGGCACTGG CCTTAATCGT TGCTACAAGA 
GGTGGCTTAC CAGGTGCAGA TGATTTATTT CAAAAACAGT TTGAATCTTT ATTGTTACAA 
AACGATTATC AAAATGCAGC TAAAGTTGCA GCCTCTTCCA CTTCTTTAAG GAACCAAAAC 
ACCATCAACA GGTTGAAAAA CATTCAAGCA CCACCAGGCG CCATTTCTCC TATCCTACTT 
TACTTTTCAA CACTATTGGA TAAAGGGAAA TTGAATAAAG AGGAAACAAT TGAATTGGCT 
AGACCAGTTC TACAACAAGA CAGAAAACAA TTGTTCGAAA AATGGTTAAA GGAAGATAAA 
CTAGAATGTT CAGAAGAACT AGGTGACATC GTTAAGCCTT TCGATACTAC TTTAGCGCTA 
GCTTGCTACT TAAGAGCAGG TGCCCATGCA AAAGTTATAT CTTGTCTTGC CGAATTACAA 
CAGTTTGAAA AAATTATTCC TTACTGCCAA AAGGTTGGAT ATCAACCTAA CTTTTTAGTG 
CTGATTTCTT CTTTGATTAG ATCATCACCA GACAGAGCTT CTGAATTTGC CGTATCTCTA 
TTACAAAATC CGGAAACTGC TTCTCAGATC GACATAGAAA AAATCGCAGA TCTATTCTTT 
TCCCAAAACC ATATCCAACA AGGAACCTCT CTACTTCTAG ACGCGTTAAA AGGAGATACC 
CCAGACCAAG GTCACTTGCA AACTCGTGTT CTTGAAGTCA ATCTACTACA CGCACCACAA 
GTTGCTGACG CCATATTGGG AAACAACATC TTTTCTCATT ATGACAAACC AACTATTGCT 
TCCTTATCCG AAAAGGCGGG ACTATACCAG AGAGCTTTAG AAAATTACAC AGACATCAAA 
GATATCAAGA GATGCGTTGT CCACACCAAT GCATTACCTA TCGATTGGTT AGTCGGATAC 
TTTGGTAAGT TGAACGTTGA ACAATCTTTG GCGTGCTTAA AAGCTTTAAT GGATAACAAT 
ATTCAGGCTA ACATCCAGAC CGTAGTACAG GTGGCAACTA AATTCTCTGA TTTGATCGGT 
CCATCTACTT TGATTAAATT ATTCGAAGAT TATAATGCTA CGGAAGGTTT ATACTATTAC 
TTAGCCTCTT TAGTCAACCT AACCGAAGAT AAAGATGTGG TTTACAAATA CATTGAAGCT 
GCTGCTAAAA TGAAACAATA CAGAGAAATT GAAAGAATTG TGAAGGATAA CAATGTCTAT 
GATCCAGAAA GAGTCAAGAA TTTCTTGAAG GATGCCAATC TAGAAGACCA GTTGCCTCTA 
GTCATAGTTT GTGACCGTTT CGACTTTGTT CATGAAATGA TTTTATATTT GTACAAATCA 
CAGAACTTAA AATTTATTGA AACTTACGTG CAGCAGGTTA ACCCTTCAAA GACTGCCCAA 
GTTGTTGGCG CATTACTTGA TATGGACTGT GATGAGGCAT TTATTCAAAG TCTGCTACAA 
TCAGTCTTAG GTCAAGTCCC TATTAATGAA TTAACTACTG AGGTTGAAAA GAGAAATAGA 
CTAAAAATAT TGCTACCTTT CCTAGAACAA AGCTTAAGTC AAGGTATCCA GGATCAAGCT 
GTTTACAATG CCTTAGCAAA AATTTATATT GATTCCAATA ATTCTCCTGA AAAGTTTTTG 
AAGGAAAACG ACCAATATGA CACCTTAGAC GTTGGTCATT ACTGCGAAAA AAGAGATCCC 
TACCTTGCAT ATATTGCGTA TGAAAAAGGT CAAAATGATG ATGACTTGAT TAGAATCACT 
AATGAAAATA GCATGTACAA GTACCAGGCA AGATATTTGT TGGAACGTTC TGATCTCGAT 
TTATGGAACA AAGTTTTGAA TCAAGAAAAT ATTCACAGAA GACAATTAAT TGATTCGGTC 
ATCTCCGTTG GTATCCCTGA ATTAACTGAT CCTGAACCTG TTTCCTTAAC TGTTCAAGCA 
TTCATGACCA ATGGATTAAA ACTGGAACTA ATTGAGTTGC TAGAAAAAAT CATATTGGAA 
CCTTCTCCTT TCAACGAAAA TGTGGCTTTA CAAGGGTTGC TATTATTGTC TGCTATTAAG 
TATGAACCAA CTAAGGTTTC AAGCTATATC GAAAAATTAG ATAACTACGA CGCCGATGAA 
ATTGCTCCAT TATGTATTGA ACATGATTTG AAGGAGGAAG CTTTTGAAAT CTATGACAAA 
CATGAAATGT ACGGTAAAGC TTTAAAGGTT TTAATCGAAG ACATCATGTC TTTAGATAGA 
GCTGCTTCTT ATGCTGATAA AATCAATACT CCCGAATTGT GGTCTCAAAT TGGTACGGCT 
CAGTTAGATG GATTAAGAAT CCCAGATGCT ATTGAGTCTT ATATTAAAGC AGAAGACCCA 
TCAAATTACG AAAATGTAAT TGATATAGCC GAACAAGCCG GTAAATACGA AGAATTAATT 
CCATTTTTAC TAATGGCTAG AAAGACTTTG AAAGAACCAA AGATCGACGG TGCTTTAATC 
TTGGCCTATG CTGAACTAAA CAAGATTCAT GAAATTGAGA ACTTGTTGGC AGGTTCCAAT 
GTTGCAAACT TAGATCATGT TGGTGATAAA TTATTCGAGA ATAAAGAGTA TAAAGCGGCC 
AGGCTATGTT ATTCTGCTGT TTCTAATTAC TCCAAGCTTG CTTCTACCTT GGTTTACCTG 
GGAGATTATC AGGCTGCTGT TGACACTGCT AGAAAGGCTT CAAACATTAA AGTTTGGAAA 
CTGGTTAACG ACGCTTGTAT TGAAAAGAAA GAATTCAAGC TGGCTCAAAT TTGTGGGTTG 
AACTTGATCG TTCACGCTGA AGAATTAGAT GAACTGGTGG AAAGGTATGA ATCAAACGGC 
TATTTCGAAG AACTGATTTC ATTGTTTGAA GCTGGTTTGG GACTTGAAAG AGCTCATATG 
GGCATGTTCA CTGAATTGGC CATTTTGTAT TCCAAATATG AACCAGACAA GACCTTCGAA 
CACTTGAAGC TATTCTGGTC CAGAATAAAT ATTCCAAAGG TTATTAGAGC TGTCGAACAA 
GCTCACCTCT GGTCGGAATT GGTCTTTTTA TATGCTCACT ATGATGAATG GGATAATGCT 
GCTTTAACAC TAATCGAAAA ATCAACCAAA GACTTGGATC ACGCCTATTT CAAAGAAGTT 
GTTGTCAAAG TTTCTAACTT AGAAATCTAT TACAAGGCCA TTAATTTCTA CGTCAAATTT 
CACCCATCAT TACTAGTTGA TCTTTTGACC TCATTGACAC CAAGATTAGA TATTCCAAGA 
ACTGTCAAAA TTTTCTCGAA ATCCGACAAC TTGCCTTTAA TCAAGCCTTT CTTGATCAAT 
GTCTTACCAA AAAATAACTC AGTTGTCAAC CAAGCCTACC ACGATCTGAT GATTGAAGAA 
GAAGATTATA AAGCTTTGCA AGACGCTGTT GATTCTTATG ACAAATTTGA CCAATTGGGA 
TTGGCATCTC GTTTGGAATC TCATAAGCTA ATTTTTTTCA AGAAAATAGG AGCCCTGTTA 
TACCGTAGAA ATAAGAAGTG GGCAAAGAGT CTTTCAATCT TGAAGGAAGA AAAGCTATGG 
AAGGACGCTA TTGAGACGGC AGCAATCTCT CAGGATCCAA AGGTAGTGGA GGCTCTACTC 
ACATATTTCG TTGAAACCGG TAACAGAGAA GGCTTTGTTG CTTTGCTATA TGCTGCTTAC 
AACTTAGTGA GAATTGAATT TGTTTTAGAA ATATCATGGA TGAATTCTTT GGAGGACTAC 
ATTAAGCCAT TTGAAATCTC CATAAAAAAA GAGCAGAATG ATTCCATTAA GAAAATCACC 
GAAGAACTCG CTAAAAAGTC CGGATCTAAT GAAGAGCACA AAGATGGCCA ACCTTTGATG 
CTGATGAACA GCGCGATGAA CGTTCAACCC ACAGGATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011309.1
CDS	1..4962
Translation	MSDLPIEFTELVDLMSLGISPQFLDFRSTTFESDHFVTVRETKDGTNSVAIVDLAKGNEVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSFTLDEPVIFWRWLSETTLGFVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFVANKNLDWFAVVGILQENGRIAGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQVFVTGNRNATTGAGELRIIEIDHDASLPSQYQKETTDIFFPPDATNDFPIAVQVSEKYGIIYLLTKYGFIHLYELETGTNLFVNRITAESVFTAAPYNHENGIACINKKGQVLAVEISTSQIVPYILNKLSNVALALIVATRGGLPGADDLFQKQFESLLLQNDYQNAAKVAASSTSLRNQNTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKEETIELARPVLQQDRKQLFEKWLKEDKLECSEELGDIVKPFDTTLALACYLRAGAHAKVISCLAELQQFEKIIPYCQKVGYQPNFLVLISSLIRSSPDRASEFAVSLLQNPETASQIDIEKIADLFFSQNHIQQGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAILGNNIFSHYDKPTIASLSEKAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGYFGKLNVEQSLACLKALMDNNIQANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYYLASLVNLTEDKDVVYKYIEAAAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPLVIVCDRFDFVHEMILYLYKSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQSVLGQVPINELTTEVEKRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFLKENDQYDTLDVGHYCEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLDLWNKVLNQENIHRRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILEPSPFNENVALQGLLLLSAIKYEPTKVSSYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDKHEMYGKALKVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDPSNYENVIDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAELNKIHEIENLLAGSNVANLDHVGDKLFENKEYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWKLVNDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHMGMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWDNAALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRLDIPRTVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYDKFDQLGLASRLESHKLIFFKKIGALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQDPKVVEALLTYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKKEQNDSIKKITEELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF

Target ORF information:

RefSeq Version	NM_001181071.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C clathrin heavy chain (CHC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181071.1

ORF Insert Sequence:

ATGAGTGACC TACCCATTGA ATTTACCGAA TTGGTCGATC TGATGTCCTT AGGAATTTCC 
CCTCAATTCC TTGACTTCAG ATCAACTACT TTCGAGAGTG ACCACTTCGT CACTGTTAGA 
GAAACAAAGG ACGGTACAAA CTCTGTTGCA ATCGTGGATT TGGCCAAAGG CAATGAAGTG 
ACAAGGAAGA ATATGGGCGG TGATTCTGCT ATCATGCATC CATCTCAGAT GGTTATTTCC 
GTTAGAGCAA ATGGTACTAT CGTGCAGATA TTTAATTTGG AAACTAAGAG CAAGTTAAAG 
TCTTTTACTT TAGATGAACC TGTTATTTTT TGGAGGTGGT TGAGTGAAAC CACTTTGGGT 
TTTGTTACCG CAAGATCCAT ACTGACCTCG AACGTCTTTG ATGGTAACGT AAATGCTAAG 
CCTCAATTGC TTACCTTGAG ACACGCTAAC TTGAACAATA CCCAAATTAT CAATTTTGTG 
GCTAACAAAA ACCTTGACTG GTTCGCAGTT GTTGGTATCT TACAAGAAAA TGGCCGTATT 
GCCGGTAGAA TTCAATTATT CTCAAAACAA CGTAACATCT CCCAAGCTAT CGACGGTCAT 
GTTGCTATCT TTACGAATAT ATTACTAGAG GGTAATGGCT CGACCCCGGT ACAAGTTTTC 
GTTACTGGTA ACAGAAATGC CACCACCGGT GCTGGAGAAT TAAGAATTAT CGAGATTGAT 
CACGATGCTT CATTGCCTTC TCAATATCAA AAGGAAACTA CCGATATTTT CTTTCCACCA 
GATGCAACGA ATGATTTCCC AATCGCTGTT CAGGTGTCTG AGAAATACGG CATAATTTAC 
CTATTAACCA AGTATGGCTT CATCCATTTG TACGAATTGG AAACCGGTAC CAATTTATTC 
GTTAATAGAA TTACAGCAGA ATCTGTTTTT ACTGCAGCTC CGTACAATCA CGAGAACGGT 
ATTGCATGCA TCAATAAAAA AGGTCAAGTT TTAGCAGTAG AGATTTCTAC TTCTCAAATC 
GTTCCATATA TCTTGAATAA ATTATCTAAT GTGGCACTGG CCTTAATCGT TGCTACAAGA 
GGTGGCTTAC CAGGTGCAGA TGATTTATTT CAAAAACAGT TTGAATCTTT ATTGTTACAA 
AACGATTATC AAAATGCAGC TAAAGTTGCA GCCTCTTCCA CTTCTTTAAG GAACCAAAAC 
ACCATCAACA GGTTGAAAAA CATTCAAGCA CCACCAGGCG CCATTTCTCC TATCCTACTT 
TACTTTTCAA CACTATTGGA TAAAGGGAAA TTGAATAAAG AGGAAACAAT TGAATTGGCT 
AGACCAGTTC TACAACAAGA CAGAAAACAA TTGTTCGAAA AATGGTTAAA GGAAGATAAA 
CTAGAATGTT CAGAAGAACT AGGTGACATC GTTAAGCCTT TCGATACTAC TTTAGCGCTA 
GCTTGCTACT TAAGAGCAGG TGCCCATGCA AAAGTTATAT CTTGTCTTGC CGAATTACAA 
CAGTTTGAAA AAATTATTCC TTACTGCCAA AAGGTTGGAT ATCAACCTAA CTTTTTAGTG 
CTGATTTCTT CTTTGATTAG ATCATCACCA GACAGAGCTT CTGAATTTGC CGTATCTCTA 
TTACAAAATC CGGAAACTGC TTCTCAGATC GACATAGAAA AAATCGCAGA TCTATTCTTT 
TCCCAAAACC ATATCCAACA AGGAACCTCT CTACTTCTAG ACGCGTTAAA AGGAGATACC 
CCAGACCAAG GTCACTTGCA AACTCGTGTT CTTGAAGTCA ATCTACTACA CGCACCACAA 
GTTGCTGACG CCATATTGGG AAACAACATC TTTTCTCATT ATGACAAACC AACTATTGCT 
TCCTTATCCG AAAAGGCGGG ACTATACCAG AGAGCTTTAG AAAATTACAC AGACATCAAA 
GATATCAAGA GATGCGTTGT CCACACCAAT GCATTACCTA TCGATTGGTT AGTCGGATAC 
TTTGGTAAGT TGAACGTTGA ACAATCTTTG GCGTGCTTAA AAGCTTTAAT GGATAACAAT 
ATTCAGGCTA ACATCCAGAC CGTAGTACAG GTGGCAACTA AATTCTCTGA TTTGATCGGT 
CCATCTACTT TGATTAAATT ATTCGAAGAT TATAATGCTA CGGAAGGTTT ATACTATTAC 
TTAGCCTCTT TAGTCAACCT AACCGAAGAT AAAGATGTGG TTTACAAATA CATTGAAGCT 
GCTGCTAAAA TGAAACAATA CAGAGAAATT GAAAGAATTG TGAAGGATAA CAATGTCTAT 
GATCCAGAAA GAGTCAAGAA TTTCTTGAAG GATGCCAATC TAGAAGACCA GTTGCCTCTA 
GTCATAGTTT GTGACCGTTT CGACTTTGTT CATGAAATGA TTTTATATTT GTACAAATCA 
CAGAACTTAA AATTTATTGA AACTTACGTG CAGCAGGTTA ACCCTTCAAA GACTGCCCAA 
GTTGTTGGCG CATTACTTGA TATGGACTGT GATGAGGCAT TTATTCAAAG TCTGCTACAA 
TCAGTCTTAG GTCAAGTCCC TATTAATGAA TTAACTACTG AGGTTGAAAA GAGAAATAGA 
CTAAAAATAT TGCTACCTTT CCTAGAACAA AGCTTAAGTC AAGGTATCCA GGATCAAGCT 
GTTTACAATG CCTTAGCAAA AATTTATATT GATTCCAATA ATTCTCCTGA AAAGTTTTTG 
AAGGAAAACG ACCAATATGA CACCTTAGAC GTTGGTCATT ACTGCGAAAA AAGAGATCCC 
TACCTTGCAT ATATTGCGTA TGAAAAAGGT CAAAATGATG ATGACTTGAT TAGAATCACT 
AATGAAAATA GCATGTACAA GTACCAGGCA AGATATTTGT TGGAACGTTC TGATCTCGAT 
TTATGGAACA AAGTTTTGAA TCAAGAAAAT ATTCACAGAA GACAATTAAT TGATTCGGTC 
ATCTCCGTTG GTATCCCTGA ATTAACTGAT CCTGAACCTG TTTCCTTAAC TGTTCAAGCA 
TTCATGACCA ATGGATTAAA ACTGGAACTA ATTGAGTTGC TAGAAAAAAT CATATTGGAA 
CCTTCTCCTT TCAACGAAAA TGTGGCTTTA CAAGGGTTGC TATTATTGTC TGCTATTAAG 
TATGAACCAA CTAAGGTTTC AAGCTATATC GAAAAATTAG ATAACTACGA CGCCGATGAA 
ATTGCTCCAT TATGTATTGA ACATGATTTG AAGGAGGAAG CTTTTGAAAT CTATGACAAA 
CATGAAATGT ACGGTAAAGC TTTAAAGGTT TTAATCGAAG ACATCATGTC TTTAGATAGA 
GCTGCTTCTT ATGCTGATAA AATCAATACT CCCGAATTGT GGTCTCAAAT TGGTACGGCT 
CAGTTAGATG GATTAAGAAT CCCAGATGCT ATTGAGTCTT ATATTAAAGC AGAAGACCCA 
TCAAATTACG AAAATGTAAT TGATATAGCC GAACAAGCCG GTAAATACGA AGAATTAATT 
CCATTTTTAC TAATGGCTAG AAAGACTTTG AAAGAACCAA AGATCGACGG TGCTTTAATC 
TTGGCCTATG CTGAACTAAA CAAGATTCAT GAAATTGAGA ACTTGTTGGC AGGTTCCAAT 
GTTGCAAACT TAGATCATGT TGGTGATAAA TTATTCGAGA ATAAAGAGTA TAAAGCGGCC 
AGGCTATGTT ATTCTGCTGT TTCTAATTAC TCCAAGCTTG CTTCTACCTT GGTTTACCTG 
GGAGATTATC AGGCTGCTGT TGACACTGCT AGAAAGGCTT CAAACATTAA AGTTTGGAAA 
CTGGTTAACG ACGCTTGTAT TGAAAAGAAA GAATTCAAGC TGGCTCAAAT TTGTGGGTTG 
AACTTGATCG TTCACGCTGA AGAATTAGAT GAACTGGTGG AAAGGTATGA ATCAAACGGC 
TATTTCGAAG AACTGATTTC ATTGTTTGAA GCTGGTTTGG GACTTGAAAG AGCTCATATG 
GGCATGTTCA CTGAATTGGC CATTTTGTAT TCCAAATATG AACCAGACAA GACCTTCGAA 
CACTTGAAGC TATTCTGGTC CAGAATAAAT ATTCCAAAGG TTATTAGAGC TGTCGAACAA 
GCTCACCTCT GGTCGGAATT GGTCTTTTTA TATGCTCACT ATGATGAATG GGATAATGCT 
GCTTTAACAC TAATCGAAAA ATCAACCAAA GACTTGGATC ACGCCTATTT CAAAGAAGTT 
GTTGTCAAAG TTTCTAACTT AGAAATCTAT TACAAGGCCA TTAATTTCTA CGTCAAATTT 
CACCCATCAT TACTAGTTGA TCTTTTGACC TCATTGACAC CAAGATTAGA TATTCCAAGA 
ACTGTCAAAA TTTTCTCGAA ATCCGACAAC TTGCCTTTAA TCAAGCCTTT CTTGATCAAT 
GTCTTACCAA AAAATAACTC AGTTGTCAAC CAAGCCTACC ACGATCTGAT GATTGAAGAA 
GAAGATTATA AAGCTTTGCA AGACGCTGTT GATTCTTATG ACAAATTTGA CCAATTGGGA 
TTGGCATCTC GTTTGGAATC TCATAAGCTA ATTTTTTTCA AGAAAATAGG AGCCCTGTTA 
TACCGTAGAA ATAAGAAGTG GGCAAAGAGT CTTTCAATCT TGAAGGAAGA AAAGCTATGG 
AAGGACGCTA TTGAGACGGC AGCAATCTCT CAGGATCCAA AGGTAGTGGA GGCTCTACTC 
ACATATTTCG TTGAAACCGG TAACAGAGAA GGCTTTGTTG CTTTGCTATA TGCTGCTTAC 
AACTTAGTGA GAATTGAATT TGTTTTAGAA ATATCATGGA TGAATTCTTT GGAGGACTAC 
ATTAAGCCAT TTGAAATCTC CATAAAAAAA GAGCAGAATG ATTCCATTAA GAAAATCACC 
GAAGAACTCG CTAAAAAGTC CGGATCTAAT GAAGAGCACA AAGATGGCCA ACCTTTGATG 
CTGATGAACA GCGCGATGAA CGTTCAACCC ACAGGATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
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Life with 6000 genes.
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CHC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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