chit-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	chit-1
Entrez Gene ID	3880403
Full Name	chitinase-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011394649.1	XP_011392951.1	chitinase-1
XM_011394648.1	XP_011392950.1	chitinase-1, variant

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chit-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chit-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf02694	XM_011394649.1 Latest version!	Neurospora crassa OR74A chitinase-1 (chit-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ONf12748	XM_011394648.1 Latest version!	Neurospora crassa OR74A chitinase-1, variant (chit-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chit-1 ( XM_011394649.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011392951.1 Neurospora crassa OR74A chitinase-1 (chit-1), mRNA.

Price & Availability↑

CloneID	ONf02694
Clone ID Related Accession (Same CDS sequence)	XM_011394649.1
Accession Version	XM_011394649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chitinase-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGCACAAAA GTCTAGTGGC AGCGGCCGTC TTGGCCGCTA CCGCCCGGGC CCACACCCTC 
ACCGAGGCCA AGGTCAACGT TTACTGGGGA CAGAGGGGAG ACGCCAGGCT CAGGGATCAC 
TGCGACCAGG CCAACTTCGA CTATGTCACT ATTGGCGCTC ACTGCGATGC CACCTACTAC 
ACGAACGGCA CAACCAGCGG GCACATGAAT GGCAAGTGCA GCGTCGTTGC GTCGGACATC 
AAGCACTGCC AGGAGAAGGG AAAGAAGGTT CTGCTTTCCC TTGATGGTGT CGAGCACATG 
GGCTCGCGCT TTTCTCTGTC CAGCGAAGCC AAGGCCGAGG AGTTTGCCAG CTTCCTGTGG 
GGAGCCTTTG GACCCTACGA TGCCAAGTGG ACGGGACCCC GTCCTTTTGA CTTTGCTGGT 
CACCGCGTCT CGGTTGATGG TTTCAACTTG GATGGCGAGC TCAAGCTCAA CGGAGCCGGC 
GAAGGTGCTT ATGCCGCCAT GGCCAAGAAG CTTCGTGAGT TGTACAACGG CAACGCCGAG 
CTCCTTCTTA CTGCCGCCCC CGGATGCTCG CTTGATGATG TCAAGATGAA GGCCATCTTC 
GACAACGCCC AGTTCGATGC CCTGTTCATC CAGTTCTACA ACAACCCTAG CTGCGAGGCT 
GCCTCGGCCT CTGGCTTCAA CTACCTCCAG TGGGAGAAGG CCATCGCCGC CGGCATGAGC 
AAGGAGGCCA AGCTCTTCAT CGGTCTTGCT GGAGCTTCTG ATGCCGCTGG CTCTGGATAT 
ATCGAGCCCC TCGAGGCTGC TGCTCTGATC AACACCTACA AGACCCGCTC CTCTTTTGGC 
GGTGCCATGG TCTTGGATGC CTTCCGCGGC CAGACTTTGG CCAATGGAAT GACTTTCCTC 
GATGTGATCA ACACTGTTGT CTCCTCCGCC GAGGCTGTTG ACCTGAGCTC TGAGTTCTGC 
GAGGACGAGT CTGCCCTTCC CAAGGTCCCC TCCGTCACCG AGGGTTCTGC CGGTGGCTTT 
TTCACTGTTG CTGACCCTTC CGCCATCACC TCTGGCCCCG TCGTTCTCCC CTCCGGTGGC 
AGCTCTGTTC TCCCCACCGG TGGTAGCTCT GAAGACGAGG TCTGCGAGGA CGAGAATGTC 
ATTCCCAAGG TCCCCTCCGT CACCGATGGC GCTCACGAGG TTAACCCCAC CATTGTTGAT 
ACTTCCATCA TCACCTCTGT CCCCGTCGCT CTCCCCTCTG GCGACCGCTC CGAGATCATT 
GGCGGCAGCC CCGCTACCGA GGACGACGTC TGCGAAGGCG AAATTATGAC CGAGGATCCT 
CTTCGCTCTG GCGTTGTTCC TAGCGGTGTT ATGCCCACCG GTGCCCTTCC TTCCGGTGTC 
CTTCCCTCTG GCGACCGCTC CGACATCATT GGCGCCATTC CCTCTGGCTC TATTCCCCTT 
GGCTCTGCTC CCTCTGGCCT CGTTCCCTCT GGCTCTATTC CCCTCGGCTC TGCTCCCTCT 
GGCCTCGTTC CCTCTGGGTC TATTCCCCTT GGCTCTGCTC CCTCTGGCCT CGTCCCCTCT 
GGCGCCGTTC CCTCTGGCTC CATTCCCCTT GGTTCCGTCC CCTCTGGCGA CCGCTCCGAC 
ATCATTGGCG CTATTCCCTC TGGATCCATT CCCCTTGGCT CCGTCCCCTC TGGCGTTGTT 
CCTTCTGGTG ACCGCTCCGA GATTATTGGC AGCCCTGCTG ATGAGGACGA CGTTTGCGAA 
GGCGAGGACC CTGTTCGCTC TGGCCTTGTC CCCTCTGGTG CCGTTCCCAC TGGCGACCGC 
TCCGACATCA TTGGCGCTAT TCCCTCTGGC TCCATTCCCC TTGGCTCTGC TCCCTCTGGC 
CTCGTTCCCT CTGGCGCTGT TCCTTCTGGT GACCGCTCTG AGATCATTGG TGCTATTCCC 
TCTGGCTCCA TTCCCCTTGG CTCCGTTCCC TCTGGCGCCG TCCCCTCTGG TGACCGCTCC 
GAGATCATTG GCGCTATTCC CTCTGGCTCC ATTCCCCTTG GCTCTGCTCC CTCTGGCGCC 
GTTCCCTCTG GCCTCGTTCC TTCTGGCATT GTTCCCTCTG GTGCCGTTCC CTCTGGCGAC 
CGCTCCGAGA TCATTGGCAG CCCCGCTGAC GAGGACGATT ATTGCGAAGG TGAGATTACG 
CCCGAGGACC CTGTTCGCTC TGGCCTTGTT CCTAGCGGCA TTGTTCCGAC TGGCGCCGTT 
CCCACTGGCC TCGTCCCCTC CGGCGGTGTC ATCGGCAGCA CCGTCCCCGG CGGTGTCATC 
CCCAGCGGCG TCGCCCCTGG CGGTATTTTG CCCTCCGGTG TCGTTTCCAG CGTTGGCTCC 
AAGGTAACCG GCCCTATCGA TGGCGATAAC GTTGCCCTCC CCAGCGTTGG TCTTCCCTCC 
GGTGGTGTTA TTGCTTCCGG TGTTGTTCCT TCCGGCGTTG TTCCCTCCGG TGTCGTTTCC 
AGCATTGGCT CTAAGGTCAC TGGTCCTATC GATGGCGATA ACGTTGCCCT CCCCAGCGTC 
GGTCTTCCCT CCGGTGCTAT TGCTTCCGGT GTTATTCCTT CCGGTGTTAT TCCTTCCGGC 
ATTGTTCCCT CCGGTGTCGT CTCCAGCATT GGCTCCAAGG TCACTGGCCC TATCGACGGC 
GATAACGTTG CTCTCCCTAG CGTTGGTCTT CCTTCTGGCG ATCTTCCTTC TGGCGTTGTC 
CCCTCCGGTG TCGTTCCCTC TGGTGTTCTT TCCAGCGTTG GTTCCAAGAT CACTGGCCCT 
ATTAATGGCC ACAACGTTGC CGTTCCTAGT GCTGGTGTTC CTTCCGGTGT TGTTCCCTCC 
GGTATCCTTT CCAACGTTGG CTCCAAGGTT ACTGGCCCTA TCGATGGCAA TAACGTTGTC 
CCTACCGGTA TCCTTCCTTC CGGCAGCGTT CCCTCCGGTG TTGTTTCTAG CGTTGTCTCC 
ATGGTCACTG CTGATCCCAT TAACGGCGAC AAGATTGCTG ACCCCTCTGC CGTCACCGCC 
CCCGCTGAGT GGACCACTTC GACCATCTAT GCCACCACCA CCTCTACTAT CACCAGCTGC 
GCTCCTGAGG TGACCGACTG CCCTGCTAAG ATCGGCCAGG TCACCACTGT TACTGTCCCC 
ATCGGCGTCA CCGTCTGCCC CGTTACCGCC ACCGAGACTG CCGCCCGCGC TCCTACTGGT 
ATCTTTACCT CGGTCCCCGC CGAGTCGATT CCTGCTGGCT TCACTACCTC GACCGTTTAC 
AGTACCACTA CCTCCACCAT CGCTAGCTGC GCTCCTGAGA TGACCGACTG CGCTGGCAAG 
ATTGGCCAAG TTACTACCGT TATTGTCCCT GTCGGCGTCA CCGTTTGCCC CGTTACCGCC 
ACCGAGACTG CCGCTACCGA GACTGCCGCC CGCGCTCCTA CGGGCATCAT GACCTCGGTC 
CCCGTCGAGT CGATCCCTGC TGGCTACACC ACCTCGACCG TCTACAGCAC TGCCACCTCC 
ACCATCGCTA GCTGTGCTCC TGAGATGACC GACTGCGCCG GCAAGATTGG CCAGGTCACC 
ACCATCATCG TTCCCGTCGG CGTTACCGTC TGCCCCATCA CCGAGGCCTT CCCTCCCGCT 
ACCTCGGTCC CCGCCGCTCC CGCCGCTGTT CCTACTGGTG CTGCTAGCGT CCCTGCTGTC 
TCCGCTGTTC CCACCGGTGG TGCGGGTGTC CCTGCCGTCT CCGCTCCCGC TGTTCCCACC 
GGTGGTGCTG GTGTTCCCGC CGTCTCCACC CCCGTTGTTC CCTCCGGTGT TGCTGGTGTT 
CCCGCCGTTT CCGCCCCCGC TGTTCCCTCC GGTGGTGCTG ATGTCCCTGC GGTCTCCGCC 
CCTGCTGTTC CCTCCGGTGG TGCTGATGTC CCTGCCGTCC CCTCCAACGG TGCCCCCGGC 
GCTGGTGTTA ACAAGGTCGC CACCTCCTCC GTCGTCTCCG TCTCCTCCAT GCCATTCACC 
ACCATCACCG TCGCCAAGCC CGCCGCTTCC GCCCCCGGTG CTCCCGGTGC CCCTGGTGCT 
GGTGTCCCCG CCGAGTCCGC GGCTGTGCCC GCCGGTGGTG CCCCTGCCGT TCCCTCCGCC 
GTCAACGCTC CTGTTGTCCC CAGCGGCGCT GTCCCCACTG GCACCGGCGT CTCGGTTGCT 
CCCTCTAGCG CTGCCTCCAC CTACAGCATG CCTGCGCCGC CTGCTCAGAC CGAGCCTGTG 
ACCGGCTCTG AGCCCTCGGA GGTTCCTGTC ACTGCTGGCG CCGGACGCAA CGTTGTTGCG 
ATGGGTGTCC CTGCTTTGGT GGCTGCTTTG GTTCTTGCTT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011392951.1
CDS	911..5215
Translation	MHKSLVAAAVLAATARAHTLTEAKVNVYWGQRGDARLRDHCDQANFDYVTIGAHCDATYYTNGTTSGHMNGKCSVVASDIKHCQEKGKKVLLSLDGVEHMGSRFSLSSEAKAEEFASFLWGAFGPYDAKWTGPRPFDFAGHRVSVDGFNLDGELKLNGAGEGAYAAMAKKLRELYNGNAELLLTAAPGCSLDDVKMKAIFDNAQFDALFIQFYNNPSCEAASASGFNYLQWEKAIAAGMSKEAKLFIGLAGASDAAGSGYIEPLEAAALINTYKTRSSFGGAMVLDAFRGQTLANGMTFLDVINTVVSSAEAVDLSSEFCEDESALPKVPSVTEGSAGGFFTVADPSAITSGPVVLPSGGSSVLPTGGSSEDEVCEDENVIPKVPSVTDGAHEVNPTIVDTSIITSVPVALPSGDRSEIIGGSPATEDDVCEGEIMTEDPLRSGVVPSGVMPTGALPSGVLPSGDRSDIIGAIPSGSIPLGSAPSGLVPSGSIPLGSAPSGLVPSGSIPLGSAPSGLVPSGAVPSGSIPLGSVPSGDRSDIIGAIPSGSIPLGSVPSGVVPSGDRSEIIGSPADEDDVCEGEDPVRSGLVPSGAVPTGDRSDIIGAIPSGSIPLGSAPSGLVPSGAVPSGDRSEIIGAIPSGSIPLGSVPSGAVPSGDRSEIIGAIPSGSIPLGSAPSGAVPSGLVPSGIVPSGAVPSGDRSEIIGSPADEDDYCEGEITPEDPVRSGLVPSGIVPTGAVPTGLVPSGGVIGSTVPGGVIPSGVAPGGILPSGVVSSVGSKVTGPIDGDNVALPSVGLPSGGVIASGVVPSGVVPSGVVSSIGSKVTGPIDGDNVALPSVGLPSGAIASGVIPSGVIPSGIVPSGVVSSIGSKVTGPIDGDNVALPSVGLPSGDLPSGVVPSGVVPSGVLSSVGSKITGPINGHNVAVPSAGVPSGVVPSGILSNVGSKVTGPIDGNNVVPTGILPSGSVPSGVVSSVVSMVTADPINGDKIADPSAVTAPAEWTTSTIYATTTSTITSCAPEVTDCPAKIGQVTTVTVPIGVTVCPVTATETAARAPTGIFTSVPAESIPAGFTTSTVYSTTTSTIASCAPEMTDCAGKIGQVTTVIVPVGVTVCPVTATETAATETAARAPTGIMTSVPVESIPAGYTTSTVYSTATSTIASCAPEMTDCAGKIGQVTTIIVPVGVTVCPITEAFPPATSVPAAPAAVPTGAASVPAVSAVPTGGAGVPAVSAPAVPTGGAGVPAVSTPVVPSGVAGVPAVSAPAVPSGGADVPAVSAPAVPSGGADVPAVPSNGAPGAGVNKVATSSVVSVSSMPFTTITVAKPAASAPGAPGAPGAGVPAESAAVPAGGAPAVPSAVNAPVVPSGAVPTGTGVSVAPSSAASTYSMPAPPAQTEPVTGSEPSEVPVTAGAGRNVVAMGVPALVAALVLAL

Target ORF information:

RefSeq Version	XM_011394649.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chitinase-1 (chit-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394649.1

ORF Insert Sequence:

ATGCACAAAA GTCTAGTGGC AGCGGCCGTC TTGGCCGCTA CCGCCCGGGC CCACACCCTC 
ACCGAGGCCA AGGTCAACGT TTACTGGGGA CAGAGGGGAG ACGCCAGGCT CAGGGATCAC 
TGCGACCAGG CCAACTTCGA CTATGTCACT ATTGGCGCTC ACTGCGATGC CACCTACTAC 
ACGAACGGCA CAACCAGCGG GCACATGAAT GGCAAGTGCA GCGTCGTTGC GTCGGACATC 
AAGCACTGCC AGGAGAAGGG AAAGAAGGTT CTGCTTTCCC TTGATGGTGT CGAGCACATG 
GGCTCGCGCT TTTCTCTGTC CAGCGAAGCC AAGGCCGAGG AGTTTGCCAG CTTCCTGTGG 
GGAGCCTTTG GACCCTACGA TGCCAAGTGG ACGGGACCCC GTCCTTTTGA CTTTGCTGGT 
CACCGCGTCT CGGTTGATGG TTTCAACTTG GATGGCGAGC TCAAGCTCAA CGGAGCCGGC 
GAAGGTGCTT ATGCCGCCAT GGCCAAGAAG CTTCGTGAGT TGTACAACGG CAACGCCGAG 
CTCCTTCTTA CTGCCGCCCC CGGATGCTCG CTTGATGATG TCAAGATGAA GGCCATCTTC 
GACAACGCCC AGTTCGATGC CCTGTTCATC CAGTTCTACA ACAACCCTAG CTGCGAGGCT 
GCCTCGGCCT CTGGCTTCAA CTACCTCCAG TGGGAGAAGG CCATCGCCGC CGGCATGAGC 
AAGGAGGCCA AGCTCTTCAT CGGTCTTGCT GGAGCTTCTG ATGCCGCTGG CTCTGGATAT 
ATCGAGCCCC TCGAGGCTGC TGCTCTGATC AACACCTACA AGACCCGCTC CTCTTTTGGC 
GGTGCCATGG TCTTGGATGC CTTCCGCGGC CAGACTTTGG CCAATGGAAT GACTTTCCTC 
GATGTGATCA ACACTGTTGT CTCCTCCGCC GAGGCTGTTG ACCTGAGCTC TGAGTTCTGC 
GAGGACGAGT CTGCCCTTCC CAAGGTCCCC TCCGTCACCG AGGGTTCTGC CGGTGGCTTT 
TTCACTGTTG CTGACCCTTC CGCCATCACC TCTGGCCCCG TCGTTCTCCC CTCCGGTGGC 
AGCTCTGTTC TCCCCACCGG TGGTAGCTCT GAAGACGAGG TCTGCGAGGA CGAGAATGTC 
ATTCCCAAGG TCCCCTCCGT CACCGATGGC GCTCACGAGG TTAACCCCAC CATTGTTGAT 
ACTTCCATCA TCACCTCTGT CCCCGTCGCT CTCCCCTCTG GCGACCGCTC CGAGATCATT 
GGCGGCAGCC CCGCTACCGA GGACGACGTC TGCGAAGGCG AAATTATGAC CGAGGATCCT 
CTTCGCTCTG GCGTTGTTCC TAGCGGTGTT ATGCCCACCG GTGCCCTTCC TTCCGGTGTC 
CTTCCCTCTG GCGACCGCTC CGACATCATT GGCGCCATTC CCTCTGGCTC TATTCCCCTT 
GGCTCTGCTC CCTCTGGCCT CGTTCCCTCT GGCTCTATTC CCCTCGGCTC TGCTCCCTCT 
GGCCTCGTTC CCTCTGGGTC TATTCCCCTT GGCTCTGCTC CCTCTGGCCT CGTCCCCTCT 
GGCGCCGTTC CCTCTGGCTC CATTCCCCTT GGTTCCGTCC CCTCTGGCGA CCGCTCCGAC 
ATCATTGGCG CTATTCCCTC TGGATCCATT CCCCTTGGCT CCGTCCCCTC TGGCGTTGTT 
CCTTCTGGTG ACCGCTCCGA GATTATTGGC AGCCCTGCTG ATGAGGACGA CGTTTGCGAA 
GGCGAGGACC CTGTTCGCTC TGGCCTTGTC CCCTCTGGTG CCGTTCCCAC TGGCGACCGC 
TCCGACATCA TTGGCGCTAT TCCCTCTGGC TCCATTCCCC TTGGCTCTGC TCCCTCTGGC 
CTCGTTCCCT CTGGCGCTGT TCCTTCTGGT GACCGCTCTG AGATCATTGG TGCTATTCCC 
TCTGGCTCCA TTCCCCTTGG CTCCGTTCCC TCTGGCGCCG TCCCCTCTGG TGACCGCTCC 
GAGATCATTG GCGCTATTCC CTCTGGCTCC ATTCCCCTTG GCTCTGCTCC CTCTGGCGCC 
GTTCCCTCTG GCCTCGTTCC TTCTGGCATT GTTCCCTCTG GTGCCGTTCC CTCTGGCGAC 
CGCTCCGAGA TCATTGGCAG CCCCGCTGAC GAGGACGATT ATTGCGAAGG TGAGATTACG 
CCCGAGGACC CTGTTCGCTC TGGCCTTGTT CCTAGCGGCA TTGTTCCGAC TGGCGCCGTT 
CCCACTGGCC TCGTCCCCTC CGGCGGTGTC ATCGGCAGCA CCGTCCCCGG CGGTGTCATC 
CCCAGCGGCG TCGCCCCTGG CGGTATTTTG CCCTCCGGTG TCGTTTCCAG CGTTGGCTCC 
AAGGTAACCG GCCCTATCGA TGGCGATAAC GTTGCCCTCC CCAGCGTTGG TCTTCCCTCC 
GGTGGTGTTA TTGCTTCCGG TGTTGTTCCT TCCGGCGTTG TTCCCTCCGG TGTCGTTTCC 
AGCATTGGCT CTAAGGTCAC TGGTCCTATC GATGGCGATA ACGTTGCCCT CCCCAGCGTC 
GGTCTTCCCT CCGGTGCTAT TGCTTCCGGT GTTATTCCTT CCGGTGTTAT TCCTTCCGGC 
ATTGTTCCCT CCGGTGTCGT CTCCAGCATT GGCTCCAAGG TCACTGGCCC TATCGACGGC 
GATAACGTTG CTCTCCCTAG CGTTGGTCTT CCTTCTGGCG ATCTTCCTTC TGGCGTTGTC 
CCCTCCGGTG TCGTTCCCTC TGGTGTTCTT TCCAGCGTTG GTTCCAAGAT CACTGGCCCT 
ATTAATGGCC ACAACGTTGC CGTTCCTAGT GCTGGTGTTC CTTCCGGTGT TGTTCCCTCC 
GGTATCCTTT CCAACGTTGG CTCCAAGGTT ACTGGCCCTA TCGATGGCAA TAACGTTGTC 
CCTACCGGTA TCCTTCCTTC CGGCAGCGTT CCCTCCGGTG TTGTTTCTAG CGTTGTCTCC 
ATGGTCACTG CTGATCCCAT TAACGGCGAC AAGATTGCTG ACCCCTCTGC CGTCACCGCC 
CCCGCTGAGT GGACCACTTC GACCATCTAT GCCACCACCA CCTCTACTAT CACCAGCTGC 
GCTCCTGAGG TGACCGACTG CCCTGCTAAG ATCGGCCAGG TCACCACTGT TACTGTCCCC 
ATCGGCGTCA CCGTCTGCCC CGTTACCGCC ACCGAGACTG CCGCCCGCGC TCCTACTGGT 
ATCTTTACCT CGGTCCCCGC CGAGTCGATT CCTGCTGGCT TCACTACCTC GACCGTTTAC 
AGTACCACTA CCTCCACCAT CGCTAGCTGC GCTCCTGAGA TGACCGACTG CGCTGGCAAG 
ATTGGCCAAG TTACTACCGT TATTGTCCCT GTCGGCGTCA CCGTTTGCCC CGTTACCGCC 
ACCGAGACTG CCGCTACCGA GACTGCCGCC CGCGCTCCTA CGGGCATCAT GACCTCGGTC 
CCCGTCGAGT CGATCCCTGC TGGCTACACC ACCTCGACCG TCTACAGCAC TGCCACCTCC 
ACCATCGCTA GCTGTGCTCC TGAGATGACC GACTGCGCCG GCAAGATTGG CCAGGTCACC 
ACCATCATCG TTCCCGTCGG CGTTACCGTC TGCCCCATCA CCGAGGCCTT CCCTCCCGCT 
ACCTCGGTCC CCGCCGCTCC CGCCGCTGTT CCTACTGGTG CTGCTAGCGT CCCTGCTGTC 
TCCGCTGTTC CCACCGGTGG TGCGGGTGTC CCTGCCGTCT CCGCTCCCGC TGTTCCCACC 
GGTGGTGCTG GTGTTCCCGC CGTCTCCACC CCCGTTGTTC CCTCCGGTGT TGCTGGTGTT 
CCCGCCGTTT CCGCCCCCGC TGTTCCCTCC GGTGGTGCTG ATGTCCCTGC GGTCTCCGCC 
CCTGCTGTTC CCTCCGGTGG TGCTGATGTC CCTGCCGTCC CCTCCAACGG TGCCCCCGGC 
GCTGGTGTTA ACAAGGTCGC CACCTCCTCC GTCGTCTCCG TCTCCTCCAT GCCATTCACC 
ACCATCACCG TCGCCAAGCC CGCCGCTTCC GCCCCCGGTG CTCCCGGTGC CCCTGGTGCT 
GGTGTCCCCG CCGAGTCCGC GGCTGTGCCC GCCGGTGGTG CCCCTGCCGT TCCCTCCGCC 
GTCAACGCTC CTGTTGTCCC CAGCGGCGCT GTCCCCACTG GCACCGGCGT CTCGGTTGCT 
CCCTCTAGCG CTGCCTCCAC CTACAGCATG CCTGCGCCGC CTGCTCAGAC CGAGCCTGTG 
ACCGGCTCTG AGCCCTCGGA GGTTCCTGTC ACTGCTGGCG CCGGACGCAA CGTTGTTGCG 
ATGGGTGTCC CTGCTTTGGT GGCTGCTTTG GTTCTTGCTT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chit-1 ( XM_011394648.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011392950.1 Neurospora crassa OR74A chitinase-1, variant (chit-1), mRNA.

Price & Availability↑

CloneID	ONf12748
Clone ID Related Accession (Same CDS sequence)	XM_011394648.1
Accession Version	XM_011394648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chitinase-1, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGAATGGCA AGTGCAGCGT CGTTGCGTCG GACATCAAGC ACTGCCAGGA GAAGGGAAAG 
AAGGTTCTGC TTTCCCTTGA TGGTGTCGAG CACATGGGCT CGCGCTTTTC TCTGTCCAGC 
GAAGCCAAGG CCGAGGAGTT TGCCAGCTTC CTGTGGGGAG CCTTTGGACC CTACGATGCC 
AAGTGGACGG GACCCCGTCC TTTTGACTTT GCTGGTCACC GCGTCTCGGT TGATGGTTTC 
AACTTGGATG GCGAGCTCAA GCTCAACGGA GCCGGCGAAG GTGCTTATGC CGCCATGGCC 
AAGAAGCTTC GTGAGTTGTA CAACGGCAAC GCCGAGCTCC TTCTTACTGC CGCCCCCGGA 
TGCTCGCTTG ATGATGTCAA GATGAAGGCC ATCTTCGACA ACGCCCAGTT CGATGCCCTG 
TTCATCCAGT TCTACAACAA CCCTAGCTGC GAGGCTGCCT CGGCCTCTGG CTTCAACTAC 
CTCCAGTGGG AGAAGGCCAT CGCCGCCGGC ATGAGCAAGG AGGCCAAGCT CTTCATCGGT 
CTTGCTGGAG CTTCTGATGC CGCTGGCTCT GGATATATCG AGCCCCTCGA GGCTGCTGCT 
CTGATCAACA CCTACAAGAC CCGCTCCTCT TTTGGCGGTG CCATGGTCTT GGATGCCTTC 
CGCGGCCAGA CTTTGGCCAA TGGAATGACT TTCCTCGATG TGATCAACAC TGTTGTCTCC 
TCCGCCGAGG CTGTTGACCT GAGCTCTGAG TTCTGCGAGG ACGAGTCTGC CCTTCCCAAG 
GTCCCCTCCG TCACCGAGGG TTCTGCCGGT GGCTTTTTCA CTGTTGCTGA CCCTTCCGCC 
ATCACCTCTG GCCCCGTCGT TCTCCCCTCC GGTGGCAGCT CTGTTCTCCC CACCGGTGGT 
AGCTCTGAAG ACGAGGTCTG CGAGGACGAG AATGTCATTC CCAAGGTCCC CTCCGTCACC 
GATGGCGCTC ACGAGGTTAA CCCCACCATT GTTGATACTT CCATCATCAC CTCTGTCCCC 
GTCGCTCTCC CCTCTGGCGA CCGCTCCGAG ATCATTGGCG GCAGCCCCGC TACCGAGGAC 
GACGTCTGCG AAGGCGAAAT TATGACCGAG GATCCTCTTC GCTCTGGCGT TGTTCCTAGC 
GGTGTTATGC CCACCGGTGC CCTTCCTTCC GGTGTCCTTC CCTCTGGCGA CCGCTCCGAC 
ATCATTGGCG CCATTCCCTC TGGCTCTATT CCCCTTGGCT CTGCTCCCTC TGGCCTCGTT 
CCCTCTGGCT CTATTCCCCT CGGCTCTGCT CCCTCTGGCC TCGTTCCCTC TGGGTCTATT 
CCCCTTGGCT CTGCTCCCTC TGGCCTCGTC CCCTCTGGCG CCGTTCCCTC TGGCTCCATT 
CCCCTTGGTT CCGTCCCCTC TGGCGACCGC TCCGACATCA TTGGCGCTAT TCCCTCTGGA 
TCCATTCCCC TTGGCTCCGT CCCCTCTGGC GTTGTTCCTT CTGGTGACCG CTCCGAGATT 
ATTGGCAGCC CTGCTGATGA GGACGACGTT TGCGAAGGCG AGGACCCTGT TCGCTCTGGC 
CTTGTCCCCT CTGGTGCCGT TCCCACTGGC GACCGCTCCG ACATCATTGG CGCTATTCCC 
TCTGGCTCCA TTCCCCTTGG CTCTGCTCCC TCTGGCCTCG TTCCCTCTGG CGCTGTTCCT 
TCTGGTGACC GCTCTGAGAT CATTGGTGCT ATTCCCTCTG GCTCCATTCC CCTTGGCTCC 
GTTCCCTCTG GCGCCGTCCC CTCTGGTGAC CGCTCCGAGA TCATTGGCGC TATTCCCTCT 
GGCTCCATTC CCCTTGGCTC TGCTCCCTCT GGCGCCGTTC CCTCTGGCCT CGTTCCTTCT 
GGCATTGTTC CCTCTGGTGC CGTTCCCTCT GGCGACCGCT CCGAGATCAT TGGCAGCCCC 
GCTGACGAGG ACGATTATTG CGAAGGTGAG ATTACGCCCG AGGACCCTGT TCGCTCTGGC 
CTTGTTCCTA GCGGCATTGT TCCGACTGGC GCCGTTCCCA CTGGCCTCGT CCCCTCCGGC 
GGTGTCATCG GCAGCACCGT CCCCGGCGGT GTCATCCCCA GCGGCGTCGC CCCTGGCGGT 
ATTTTGCCCT CCGGTGTCGT TTCCAGCGTT GGCTCCAAGG TAACCGGCCC TATCGATGGC 
GATAACGTTG CCCTCCCCAG CGTTGGTCTT CCCTCCGGTG GTGTTATTGC TTCCGGTGTT 
GTTCCTTCCG GCGTTGTTCC CTCCGGTGTC GTTTCCAGCA TTGGCTCTAA GGTCACTGGT 
CCTATCGATG GCGATAACGT TGCCCTCCCC AGCGTCGGTC TTCCCTCCGG TGCTATTGCT 
TCCGGTGTTA TTCCTTCCGG TGTTATTCCT TCCGGCATTG TTCCCTCCGG TGTCGTCTCC 
AGCATTGGCT CCAAGGTCAC TGGCCCTATC GACGGCGATA ACGTTGCTCT CCCTAGCGTT 
GGTCTTCCTT CTGGCGATCT TCCTTCTGGC GTTGTCCCCT CCGGTGTCGT TCCCTCTGGT 
GTTCTTTCCA GCGTTGGTTC CAAGATCACT GGCCCTATTA ATGGCCACAA CGTTGCCGTT 
CCTAGTGCTG GTGTTCCTTC CGGTGTTGTT CCCTCCGGTA TCCTTTCCAA CGTTGGCTCC 
AAGGTTACTG GCCCTATCGA TGGCAATAAC GTTGTCCCTA CCGGTATCCT TCCTTCCGGC 
AGCGTTCCCT CCGGTGTTGT TTCTAGCGTT GTCTCCATGG TCACTGCTGA TCCCATTAAC 
GGCGACAAGA TTGCTGACCC CTCTGCCGTC ACCGCCCCCG CTGAGTGGAC CACTTCGACC 
ATCTATGCCA CCACCACCTC TACTATCACC AGCTGCGCTC CTGAGGTGAC CGACTGCCCT 
GCTAAGATCG GCCAGGTCAC CACTGTTACT GTCCCCATCG GCGTCACCGT CTGCCCCGTT 
ACCGCCACCG AGACTGCCGC CCGCGCTCCT ACTGGTATCT TTACCTCGGT CCCCGCCGAG 
TCGATTCCTG CTGGCTTCAC TACCTCGACC GTTTACAGTA CCACTACCTC CACCATCGCT 
AGCTGCGCTC CTGAGATGAC CGACTGCGCT GGCAAGATTG GCCAAGTTAC TACCGTTATT 
GTCCCTGTCG GCGTCACCGT TTGCCCCGTT ACCGCCACCG AGACTGCCGC TACCGAGACT 
GCCGCCCGCG CTCCTACGGG CATCATGACC TCGGTCCCCG TCGAGTCGAT CCCTGCTGGC 
TACACCACCT CGACCGTCTA CAGCACTGCC ACCTCCACCA TCGCTAGCTG TGCTCCTGAG 
ATGACCGACT GCGCCGGCAA GATTGGCCAG GTCACCACCA TCATCGTTCC CGTCGGCGTT 
ACCGTCTGCC CCATCACCGA GGCCTTCCCT CCCGCTACCT CGGTCCCCGC CGCTCCCGCC 
GCTGTTCCTA CTGGTGCTGC TAGCGTCCCT GCTGTCTCCG CTGTTCCCAC CGGTGGTGCG 
GGTGTCCCTG CCGTCTCCGC TCCCGCTGTT CCCACCGGTG GTGCTGGTGT TCCCGCCGTC 
TCCACCCCCG TTGTTCCCTC CGGTGTTGCT GGTGTTCCCG CCGTTTCCGC CCCCGCTGTT 
CCCTCCGGTG GTGCTGATGT CCCTGCGGTC TCCGCCCCTG CTGTTCCCTC CGGTGGTGCT 
GATGTCCCTG CCGTCCCCTC CAACGGTGCC CCCGGCGCTG GTGTTAACAA GGTCGCCACC 
TCCTCCGTCG TCTCCGTCTC CTCCATGCCA TTCACCACCA TCACCGTCGC CAAGCCCGCC 
GCTTCCGCCC CCGGTGCTCC CGGTGCCCCT GGTGCTGGTG TCCCCGCCGA GTCCGCGGCT 
GTGCCCGCCG GTGGTGCCCC TGCCGTTCCC TCCGCCGTCA ACGCTCCTGT TGTCCCCAGC 
GGCGCTGTCC CCACTGGCAC CGGCGTCTCG GTTGCTCCCT CTAGCGCTGC CTCCACCTAC 
AGCATGCCTG CGCCGCCTGC TCAGACCGAG CCTGTGACCG GCTCTGAGCC CTCGGAGGTT 
CCTGTCACTG CTGGCGCCGG ACGCAACGTT GTTGCGATGG GTGTCCCTGC TTTGGTGGCT 
GCTTTGGTTC TTGCTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011392950.1
CDS	887..4987
Translation	MNGKCSVVASDIKHCQEKGKKVLLSLDGVEHMGSRFSLSSEAKAEEFASFLWGAFGPYDAKWTGPRPFDFAGHRVSVDGFNLDGELKLNGAGEGAYAAMAKKLRELYNGNAELLLTAAPGCSLDDVKMKAIFDNAQFDALFIQFYNNPSCEAASASGFNYLQWEKAIAAGMSKEAKLFIGLAGASDAAGSGYIEPLEAAALINTYKTRSSFGGAMVLDAFRGQTLANGMTFLDVINTVVSSAEAVDLSSEFCEDESALPKVPSVTEGSAGGFFTVADPSAITSGPVVLPSGGSSVLPTGGSSEDEVCEDENVIPKVPSVTDGAHEVNPTIVDTSIITSVPVALPSGDRSEIIGGSPATEDDVCEGEIMTEDPLRSGVVPSGVMPTGALPSGVLPSGDRSDIIGAIPSGSIPLGSAPSGLVPSGSIPLGSAPSGLVPSGSIPLGSAPSGLVPSGAVPSGSIPLGSVPSGDRSDIIGAIPSGSIPLGSVPSGVVPSGDRSEIIGSPADEDDVCEGEDPVRSGLVPSGAVPTGDRSDIIGAIPSGSIPLGSAPSGLVPSGAVPSGDRSEIIGAIPSGSIPLGSVPSGAVPSGDRSEIIGAIPSGSIPLGSAPSGAVPSGLVPSGIVPSGAVPSGDRSEIIGSPADEDDYCEGEITPEDPVRSGLVPSGIVPTGAVPTGLVPSGGVIGSTVPGGVIPSGVAPGGILPSGVVSSVGSKVTGPIDGDNVALPSVGLPSGGVIASGVVPSGVVPSGVVSSIGSKVTGPIDGDNVALPSVGLPSGAIASGVIPSGVIPSGIVPSGVVSSIGSKVTGPIDGDNVALPSVGLPSGDLPSGVVPSGVVPSGVLSSVGSKITGPINGHNVAVPSAGVPSGVVPSGILSNVGSKVTGPIDGNNVVPTGILPSGSVPSGVVSSVVSMVTADPINGDKIADPSAVTAPAEWTTSTIYATTTSTITSCAPEVTDCPAKIGQVTTVTVPIGVTVCPVTATETAARAPTGIFTSVPAESIPAGFTTSTVYSTTTSTIASCAPEMTDCAGKIGQVTTVIVPVGVTVCPVTATETAATETAARAPTGIMTSVPVESIPAGYTTSTVYSTATSTIASCAPEMTDCAGKIGQVTTIIVPVGVTVCPITEAFPPATSVPAAPAAVPTGAASVPAVSAVPTGGAGVPAVSAPAVPTGGAGVPAVSTPVVPSGVAGVPAVSAPAVPSGGADVPAVSAPAVPSGGADVPAVPSNGAPGAGVNKVATSSVVSVSSMPFTTITVAKPAASAPGAPGAPGAGVPAESAAVPAGGAPAVPSAVNAPVVPSGAVPTGTGVSVAPSSAASTYSMPAPPAQTEPVTGSEPSEVPVTAGAGRNVVAMGVPALVAALVLAL

Target ORF information:

RefSeq Version	XM_011394648.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chitinase-1, variant (chit-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394648.1

ORF Insert Sequence:

ATGAATGGCA AGTGCAGCGT CGTTGCGTCG GACATCAAGC ACTGCCAGGA GAAGGGAAAG 
AAGGTTCTGC TTTCCCTTGA TGGTGTCGAG CACATGGGCT CGCGCTTTTC TCTGTCCAGC 
GAAGCCAAGG CCGAGGAGTT TGCCAGCTTC CTGTGGGGAG CCTTTGGACC CTACGATGCC 
AAGTGGACGG GACCCCGTCC TTTTGACTTT GCTGGTCACC GCGTCTCGGT TGATGGTTTC 
AACTTGGATG GCGAGCTCAA GCTCAACGGA GCCGGCGAAG GTGCTTATGC CGCCATGGCC 
AAGAAGCTTC GTGAGTTGTA CAACGGCAAC GCCGAGCTCC TTCTTACTGC CGCCCCCGGA 
TGCTCGCTTG ATGATGTCAA GATGAAGGCC ATCTTCGACA ACGCCCAGTT CGATGCCCTG 
TTCATCCAGT TCTACAACAA CCCTAGCTGC GAGGCTGCCT CGGCCTCTGG CTTCAACTAC 
CTCCAGTGGG AGAAGGCCAT CGCCGCCGGC ATGAGCAAGG AGGCCAAGCT CTTCATCGGT 
CTTGCTGGAG CTTCTGATGC CGCTGGCTCT GGATATATCG AGCCCCTCGA GGCTGCTGCT 
CTGATCAACA CCTACAAGAC CCGCTCCTCT TTTGGCGGTG CCATGGTCTT GGATGCCTTC 
CGCGGCCAGA CTTTGGCCAA TGGAATGACT TTCCTCGATG TGATCAACAC TGTTGTCTCC 
TCCGCCGAGG CTGTTGACCT GAGCTCTGAG TTCTGCGAGG ACGAGTCTGC CCTTCCCAAG 
GTCCCCTCCG TCACCGAGGG TTCTGCCGGT GGCTTTTTCA CTGTTGCTGA CCCTTCCGCC 
ATCACCTCTG GCCCCGTCGT TCTCCCCTCC GGTGGCAGCT CTGTTCTCCC CACCGGTGGT 
AGCTCTGAAG ACGAGGTCTG CGAGGACGAG AATGTCATTC CCAAGGTCCC CTCCGTCACC 
GATGGCGCTC ACGAGGTTAA CCCCACCATT GTTGATACTT CCATCATCAC CTCTGTCCCC 
GTCGCTCTCC CCTCTGGCGA CCGCTCCGAG ATCATTGGCG GCAGCCCCGC TACCGAGGAC 
GACGTCTGCG AAGGCGAAAT TATGACCGAG GATCCTCTTC GCTCTGGCGT TGTTCCTAGC 
GGTGTTATGC CCACCGGTGC CCTTCCTTCC GGTGTCCTTC CCTCTGGCGA CCGCTCCGAC 
ATCATTGGCG CCATTCCCTC TGGCTCTATT CCCCTTGGCT CTGCTCCCTC TGGCCTCGTT 
CCCTCTGGCT CTATTCCCCT CGGCTCTGCT CCCTCTGGCC TCGTTCCCTC TGGGTCTATT 
CCCCTTGGCT CTGCTCCCTC TGGCCTCGTC CCCTCTGGCG CCGTTCCCTC TGGCTCCATT 
CCCCTTGGTT CCGTCCCCTC TGGCGACCGC TCCGACATCA TTGGCGCTAT TCCCTCTGGA 
TCCATTCCCC TTGGCTCCGT CCCCTCTGGC GTTGTTCCTT CTGGTGACCG CTCCGAGATT 
ATTGGCAGCC CTGCTGATGA GGACGACGTT TGCGAAGGCG AGGACCCTGT TCGCTCTGGC 
CTTGTCCCCT CTGGTGCCGT TCCCACTGGC GACCGCTCCG ACATCATTGG CGCTATTCCC 
TCTGGCTCCA TTCCCCTTGG CTCTGCTCCC TCTGGCCTCG TTCCCTCTGG CGCTGTTCCT 
TCTGGTGACC GCTCTGAGAT CATTGGTGCT ATTCCCTCTG GCTCCATTCC CCTTGGCTCC 
GTTCCCTCTG GCGCCGTCCC CTCTGGTGAC CGCTCCGAGA TCATTGGCGC TATTCCCTCT 
GGCTCCATTC CCCTTGGCTC TGCTCCCTCT GGCGCCGTTC CCTCTGGCCT CGTTCCTTCT 
GGCATTGTTC CCTCTGGTGC CGTTCCCTCT GGCGACCGCT CCGAGATCAT TGGCAGCCCC 
GCTGACGAGG ACGATTATTG CGAAGGTGAG ATTACGCCCG AGGACCCTGT TCGCTCTGGC 
CTTGTTCCTA GCGGCATTGT TCCGACTGGC GCCGTTCCCA CTGGCCTCGT CCCCTCCGGC 
GGTGTCATCG GCAGCACCGT CCCCGGCGGT GTCATCCCCA GCGGCGTCGC CCCTGGCGGT 
ATTTTGCCCT CCGGTGTCGT TTCCAGCGTT GGCTCCAAGG TAACCGGCCC TATCGATGGC 
GATAACGTTG CCCTCCCCAG CGTTGGTCTT CCCTCCGGTG GTGTTATTGC TTCCGGTGTT 
GTTCCTTCCG GCGTTGTTCC CTCCGGTGTC GTTTCCAGCA TTGGCTCTAA GGTCACTGGT 
CCTATCGATG GCGATAACGT TGCCCTCCCC AGCGTCGGTC TTCCCTCCGG TGCTATTGCT 
TCCGGTGTTA TTCCTTCCGG TGTTATTCCT TCCGGCATTG TTCCCTCCGG TGTCGTCTCC 
AGCATTGGCT CCAAGGTCAC TGGCCCTATC GACGGCGATA ACGTTGCTCT CCCTAGCGTT 
GGTCTTCCTT CTGGCGATCT TCCTTCTGGC GTTGTCCCCT CCGGTGTCGT TCCCTCTGGT 
GTTCTTTCCA GCGTTGGTTC CAAGATCACT GGCCCTATTA ATGGCCACAA CGTTGCCGTT 
CCTAGTGCTG GTGTTCCTTC CGGTGTTGTT CCCTCCGGTA TCCTTTCCAA CGTTGGCTCC 
AAGGTTACTG GCCCTATCGA TGGCAATAAC GTTGTCCCTA CCGGTATCCT TCCTTCCGGC 
AGCGTTCCCT CCGGTGTTGT TTCTAGCGTT GTCTCCATGG TCACTGCTGA TCCCATTAAC 
GGCGACAAGA TTGCTGACCC CTCTGCCGTC ACCGCCCCCG CTGAGTGGAC CACTTCGACC 
ATCTATGCCA CCACCACCTC TACTATCACC AGCTGCGCTC CTGAGGTGAC CGACTGCCCT 
GCTAAGATCG GCCAGGTCAC CACTGTTACT GTCCCCATCG GCGTCACCGT CTGCCCCGTT 
ACCGCCACCG AGACTGCCGC CCGCGCTCCT ACTGGTATCT TTACCTCGGT CCCCGCCGAG 
TCGATTCCTG CTGGCTTCAC TACCTCGACC GTTTACAGTA CCACTACCTC CACCATCGCT 
AGCTGCGCTC CTGAGATGAC CGACTGCGCT GGCAAGATTG GCCAAGTTAC TACCGTTATT 
GTCCCTGTCG GCGTCACCGT TTGCCCCGTT ACCGCCACCG AGACTGCCGC TACCGAGACT 
GCCGCCCGCG CTCCTACGGG CATCATGACC TCGGTCCCCG TCGAGTCGAT CCCTGCTGGC 
TACACCACCT CGACCGTCTA CAGCACTGCC ACCTCCACCA TCGCTAGCTG TGCTCCTGAG 
ATGACCGACT GCGCCGGCAA GATTGGCCAG GTCACCACCA TCATCGTTCC CGTCGGCGTT 
ACCGTCTGCC CCATCACCGA GGCCTTCCCT CCCGCTACCT CGGTCCCCGC CGCTCCCGCC 
GCTGTTCCTA CTGGTGCTGC TAGCGTCCCT GCTGTCTCCG CTGTTCCCAC CGGTGGTGCG 
GGTGTCCCTG CCGTCTCCGC TCCCGCTGTT CCCACCGGTG GTGCTGGTGT TCCCGCCGTC 
TCCACCCCCG TTGTTCCCTC CGGTGTTGCT GGTGTTCCCG CCGTTTCCGC CCCCGCTGTT 
CCCTCCGGTG GTGCTGATGT CCCTGCGGTC TCCGCCCCTG CTGTTCCCTC CGGTGGTGCT 
GATGTCCCTG CCGTCCCCTC CAACGGTGCC CCCGGCGCTG GTGTTAACAA GGTCGCCACC 
TCCTCCGTCG TCTCCGTCTC CTCCATGCCA TTCACCACCA TCACCGTCGC CAAGCCCGCC 
GCTTCCGCCC CCGGTGCTCC CGGTGCCCCT GGTGCTGGTG TCCCCGCCGA GTCCGCGGCT 
GTGCCCGCCG GTGGTGCCCC TGCCGTTCCC TCCGCCGTCA ACGCTCCTGT TGTCCCCAGC 
GGCGCTGTCC CCACTGGCAC CGGCGTCTCG GTTGCTCCCT CTAGCGCTGC CTCCACCTAC 
AGCATGCCTG CGCCGCCTGC TCAGACCGAG CCTGTGACCG GCTCTGAGCC CTCGGAGGTT 
CCTGTCACTG CTGGCGCCGG ACGCAACGTT GTTGCGATGG GTGTCCCTGC TTTGGTGGCT 
GCTTTGGTTC TTGCTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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chit-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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