sad-2 cDNA ORF clone, Neurospora crassa OR74A

The following sad-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sad-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf11657 XM_955991.2
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Neurospora crassa OR74A SAD-2 (sad-2), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf11657
    Clone ID Related Accession (Same CDS sequence) XM_955991.2
    Accession Version XM_955991.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product SAD-2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505). On Feb 23, 2015 this sequence version replaced XM_955991.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACGGACC GCGACAAGAA TGGCCAATCG GCCGCCGACG AAGAGGAGCA GAAGAGAAAA 
    GCCATTCTGG CAAAGGCCAT GATGGATGAC CTCGGCACTG CTCGCGTCGA AAACCTTCCG
    TTGGAGGATG TTGACCTCCC TCCTCGTCAT GGTCGTCATG ATGGCCGGGG ACACTCTGGT
    CTTACTATGC CTCGACCTGC TCCCGCTGTG TCTGATATCT GGGCAGAGGC CCAACGACAG
    GGCGTTTTCA ACGATGATGA CGCTATTGCT GTGCGAGAAC TTGATGATCT TGGAGGCGGA
    AGAATCTATG CCACCCGCCA GCAGGAAGTC AGCGCTTTGG CTAGCCGCCT CGTTCGTGGC
    GTCAGAGATC ATGCTCAAAA AGTATTGAAC CCCGAGCTGG ACAGCCGCCT TGCTAAACAA
    TACCATGGCC GCGGACGCGA GATACCCCCA AGCAGAAAAA TCAACATTAG CAAAGACATG
    AAAACATCCA CTCTCGGTGT CCTTTACCAT GCATCTCAGC CGGTCAGGCA ATTCCCCCCA
    AACTACCGCC CATCTCCTAC ATCACCCCAT CCATTGGCTA CCACAAAGCC CCTTCAACAA
    GTCCCTTCTA GTCAAGGACT TTCGTCTTCC CGGGTTCCAG TTGCGTCTTG TGCATCTTCT
    ACTCCGCCTA TCAAAGAAAC CTGTCGAGCT ACTTCGGTCC AGTCAACTCG CCACGCAACT
    ATCAGTCAAG GACCCCGTCA TCCACCTGTC AACCAGGCTC AAACACAGCC TGGCCTAGCT
    ACCGCGTCAA CTGATTTACG CCAAAGCTCC GCGAGGATCC GACAACCGTC TACGTCCACC
    ACTCAAGGCC CAGCAAGTCA GCAACTCCTG TTTTTGGGCA ATGAACATAT TGTTCCCCCT
    TCAGGGAATG AATCTTCTCC ACTTTCGCCG GAGCTGCCTA ACGTTCTCTT CAAATGCGAA
    GTTGACTTTC CTAATCCAAC ACGTCTGCCT GACAGAACGC TTGTACCCTC CATGGTTTAT
    CTTTCTGCGG CCGGAAAACC ACATATGGGG TTCTTTACAC TGACTTGCGA AGGCAAGCAA
    GTCTGCCGAT CGCCTATCTC TCAATATTAC CATCATACTA TCGACTCTGG ATCCCGACAA
    CTGATAATTA TTTTCAGAGG CCAAGAGGGT TATACTAGCA GTCACATAGT CAACTTCATG
    GCTTATATGG ACGCGGAAGA TTTTCACAGG ACACTCACCC GTCTTCGTGC TGGCAAATAT
    CTGGACCAGG TTAATGACAG CTCCAGGCAG ATGTTGGAGG CTACCATTCA AGAAGCAGCT
    CCAACTACCG TGGAGACATC TCCCACGCCG GCTCCTCGGA ACCCCGTGTC TCAAGCTCGA
    GCGGAACAAG CAATCACAAC AGATGTTGAC ACGGTCTCAA GACAACCATA TGCACCAGAT
    TCAACCACAA CCCAACGTGC AGAGAACCAG GAGATACTGG TTGGTACGCT TATTGACTTG
    GACACTTTGG ATGACGCTCC ATCGACGGTC CGGCCACAGT ATGCTCAATC TGAAGCTGTG
    GAGCTGCTTT CTACTCTTGA TCCGTTTGAG TATGAGCTTG AACATGTCGA CGATCAGCTT
    CAGGTCCAGC AAGCGCAATC TGGAATCACG GAACAGAACT ATGGGCTTGA TATTGTCAAG
    CAGGAGTTTG TTGATACATA CCGGTTACTG TTGCAGAACA TGATTAAGGT ATTGGGTGAA
    ATGCCGAGCA ACAGGACGAA TCGCGGTGCT TCTACGATGA TTGAAGGACT CCAGACATTT
    GTGACGCAAT CAGCAATGGA TGATGAATGC GGTCTCGATG AATCAACCAA AAAAGAGCTT
    CTGAAGGAGG TCTGGGGCGA CTCTCAACCT GTGGATACCG ACAATGTTAC CAACGACATT
    CAATCGGCTC CCAATTCTCT GCCAGATCGC CCGAAAACTC CCAGACCTCC CCCTCAGACC
    GCAAGAACAA CTTCTCATGA TACTAGCAGA GCACACTCCC CTGTAGCCAC TACGCATACT
    CGCCGCCGTG TGTACCAGCT CCCATTCCTT GTGTCCCTTT ACAACAATCG TTTGCAGCCT
    CCCCACTGGC TCAGTGAGCT GGGATTTATG CCCGCGTTCT CTAGAAGGAA CCAGCCTCCC
    TCGACGTGTC AGAGCTTGGA AGCTACGCTT ACAGCCACGA GGCCTGCCAC TCCGCTTCAA
    GCGGCTGCAA TTCTGGCTCC TGATTTTACC AGGAGCAGAG CCAATCACGC CTGGGTGATG
    GCAACAGAGG CCTCAGAGAC CGTAGAGCCT ACGCCCATCA TAACTTCCGA GTCAACGACT
    CAAGATGTGC AAGCAGAGAC CCAGGTATCG CAGATCAGAG AGCATACATC GGTAGTTCCC
    AATGAGACAG CTACGAGTCT ATCCCATGAA GAGCACATAA ATGCAAATAT CTGCAGTGAC
    CCCGAGCTCA TCTCCTTGGC CACTCCAAAG CCATCAAGGC CTCCACCAAC ACAGATGAAT
    GTTCGTGGTC TTCGAAACTC ATTGTGGGCT AGACCTGATG ATCGCCTTGA AACTGAAGGA
    GTTTTTACAG GTCCTCAATT CTTGACTAGT GCCACCCTTC GAGCGCGAGC GCAACTTGAT
    CCACAGGCCC CAGTCAGTGC TACTCCTACC GAGTTGGCGG AAATGTTCTC TGGTAGGCCC
    TCTACTCAGA GTATTGAGCA TGTCACTCCT TACATTGCAC CTGGTGTGCA AAATAATGGA
    CCCATTTCAG TGTCTAATCA CCGGCCCGCC ACCCCTGCCG ACAATACTTC AAGTGTTGGA
    GGACCGACTC CTCAGGATCA AACATCTCTT TCAACCCAGC GTGTTGTTAA CACGCCTATT
    CAGCAGCCAA GCTCCATCAT CGCACCGCCT GCTCAATCGC AGTATCCGGT CATCTCTAGC
    ATTCTTTCCA ACCGTAGCCT GGCTGGCTCA GGGTATGTGA CTAGTTTGGT GCCAGAGAGT
    TCGTCCATAC CAGCTCTCCC TCAACCCGAG AAATCTGCTT CTTCAAGCAG CTCCACCAAC
    CCTGGTTTAG CCGGCTCTAG GTTTGCTAGC AGTTCAGCAC CAGAAAACTC TAATGGTACC
    GACTTTCCCG TTGTTCAAGT CCAATCGCCG GTTCCTGTTT TCGAGGCCCA ACCGACGGCT
    CTCGCTCCTA GACCTATCAA CCGCGGTTTG GCCGGATCAA GATTCGCTTC TGGCAAGGTG
    CTATCCAGCT CTGGTATCTT TACGGGCCAC TACGCTCGAA CGCAGCCACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_961084.2
    CDS1..3294
    Translation

    Target ORF information:

    RefSeq Version XM_955991.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A SAD-2 (sad-2), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_955991.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACGGACC GCGACAAGAA TGGCCAATCG GCCGCCGACG AAGAGGAGCA GAAGAGAAAA 
    GCCATTCTGG CAAAGGCCAT GATGGATGAC CTCGGCACTG CTCGCGTCGA AAACCTTCCG
    TTGGAGGATG TTGACCTCCC TCCTCGTCAT GGTCGTCATG ATGGCCGGGG ACACTCTGGT
    CTTACTATGC CTCGACCTGC TCCCGCTGTG TCTGATATCT GGGCAGAGGC CCAACGACAG
    GGCGTTTTCA ACGATGATGA CGCTATTGCT GTGCGAGAAC TTGATGATCT TGGAGGCGGA
    AGAATCTATG CCACCCGCCA GCAGGAAGTC AGCGCTTTGG CTAGCCGCCT CGTTCGTGGC
    GTCAGAGATC ATGCTCAAAA AGTATTGAAC CCCGAGCTGG ACAGCCGCCT TGCTAAACAA
    TACCATGGCC GCGGACGCGA GATACCCCCA AGCAGAAAAA TCAACATTAG CAAAGACATG
    AAAACATCCA CTCTCGGTGT CCTTTACCAT GCATCTCAGC CGGTCAGGCA ATTCCCCCCA
    AACTACCGCC CATCTCCTAC ATCACCCCAT CCATTGGCTA CCACAAAGCC CCTTCAACAA
    GTCCCTTCTA GTCAAGGACT TTCGTCTTCC CGGGTTCCAG TTGCGTCTTG TGCATCTTCT
    ACTCCGCCTA TCAAAGAAAC CTGTCGAGCT ACTTCGGTCC AGTCAACTCG CCACGCAACT
    ATCAGTCAAG GACCCCGTCA TCCACCTGTC AACCAGGCTC AAACACAGCC TGGCCTAGCT
    ACCGCGTCAA CTGATTTACG CCAAAGCTCC GCGAGGATCC GACAACCGTC TACGTCCACC
    ACTCAAGGCC CAGCAAGTCA GCAACTCCTG TTTTTGGGCA ATGAACATAT TGTTCCCCCT
    TCAGGGAATG AATCTTCTCC ACTTTCGCCG GAGCTGCCTA ACGTTCTCTT CAAATGCGAA
    GTTGACTTTC CTAATCCAAC ACGTCTGCCT GACAGAACGC TTGTACCCTC CATGGTTTAT
    CTTTCTGCGG CCGGAAAACC ACATATGGGG TTCTTTACAC TGACTTGCGA AGGCAAGCAA
    GTCTGCCGAT CGCCTATCTC TCAATATTAC CATCATACTA TCGACTCTGG ATCCCGACAA
    CTGATAATTA TTTTCAGAGG CCAAGAGGGT TATACTAGCA GTCACATAGT CAACTTCATG
    GCTTATATGG ACGCGGAAGA TTTTCACAGG ACACTCACCC GTCTTCGTGC TGGCAAATAT
    CTGGACCAGG TTAATGACAG CTCCAGGCAG ATGTTGGAGG CTACCATTCA AGAAGCAGCT
    CCAACTACCG TGGAGACATC TCCCACGCCG GCTCCTCGGA ACCCCGTGTC TCAAGCTCGA
    GCGGAACAAG CAATCACAAC AGATGTTGAC ACGGTCTCAA GACAACCATA TGCACCAGAT
    TCAACCACAA CCCAACGTGC AGAGAACCAG GAGATACTGG TTGGTACGCT TATTGACTTG
    GACACTTTGG ATGACGCTCC ATCGACGGTC CGGCCACAGT ATGCTCAATC TGAAGCTGTG
    GAGCTGCTTT CTACTCTTGA TCCGTTTGAG TATGAGCTTG AACATGTCGA CGATCAGCTT
    CAGGTCCAGC AAGCGCAATC TGGAATCACG GAACAGAACT ATGGGCTTGA TATTGTCAAG
    CAGGAGTTTG TTGATACATA CCGGTTACTG TTGCAGAACA TGATTAAGGT ATTGGGTGAA
    ATGCCGAGCA ACAGGACGAA TCGCGGTGCT TCTACGATGA TTGAAGGACT CCAGACATTT
    GTGACGCAAT CAGCAATGGA TGATGAATGC GGTCTCGATG AATCAACCAA AAAAGAGCTT
    CTGAAGGAGG TCTGGGGCGA CTCTCAACCT GTGGATACCG ACAATGTTAC CAACGACATT
    CAATCGGCTC CCAATTCTCT GCCAGATCGC CCGAAAACTC CCAGACCTCC CCCTCAGACC
    GCAAGAACAA CTTCTCATGA TACTAGCAGA GCACACTCCC CTGTAGCCAC TACGCATACT
    CGCCGCCGTG TGTACCAGCT CCCATTCCTT GTGTCCCTTT ACAACAATCG TTTGCAGCCT
    CCCCACTGGC TCAGTGAGCT GGGATTTATG CCCGCGTTCT CTAGAAGGAA CCAGCCTCCC
    TCGACGTGTC AGAGCTTGGA AGCTACGCTT ACAGCCACGA GGCCTGCCAC TCCGCTTCAA
    GCGGCTGCAA TTCTGGCTCC TGATTTTACC AGGAGCAGAG CCAATCACGC CTGGGTGATG
    GCAACAGAGG CCTCAGAGAC CGTAGAGCCT ACGCCCATCA TAACTTCCGA GTCAACGACT
    CAAGATGTGC AAGCAGAGAC CCAGGTATCG CAGATCAGAG AGCATACATC GGTAGTTCCC
    AATGAGACAG CTACGAGTCT ATCCCATGAA GAGCACATAA ATGCAAATAT CTGCAGTGAC
    CCCGAGCTCA TCTCCTTGGC CACTCCAAAG CCATCAAGGC CTCCACCAAC ACAGATGAAT
    GTTCGTGGTC TTCGAAACTC ATTGTGGGCT AGACCTGATG ATCGCCTTGA AACTGAAGGA
    GTTTTTACAG GTCCTCAATT CTTGACTAGT GCCACCCTTC GAGCGCGAGC GCAACTTGAT
    CCACAGGCCC CAGTCAGTGC TACTCCTACC GAGTTGGCGG AAATGTTCTC TGGTAGGCCC
    TCTACTCAGA GTATTGAGCA TGTCACTCCT TACATTGCAC CTGGTGTGCA AAATAATGGA
    CCCATTTCAG TGTCTAATCA CCGGCCCGCC ACCCCTGCCG ACAATACTTC AAGTGTTGGA
    GGACCGACTC CTCAGGATCA AACATCTCTT TCAACCCAGC GTGTTGTTAA CACGCCTATT
    CAGCAGCCAA GCTCCATCAT CGCACCGCCT GCTCAATCGC AGTATCCGGT CATCTCTAGC
    ATTCTTTCCA ACCGTAGCCT GGCTGGCTCA GGGTATGTGA CTAGTTTGGT GCCAGAGAGT
    TCGTCCATAC CAGCTCTCCC TCAACCCGAG AAATCTGCTT CTTCAAGCAG CTCCACCAAC
    CCTGGTTTAG CCGGCTCTAG GTTTGCTAGC AGTTCAGCAC CAGAAAACTC TAATGGTACC
    GACTTTCCCG TTGTTCAAGT CCAATCGCCG GTTCCTGTTT TCGAGGCCCA ACCGACGGCT
    CTCGCTCCTA GACCTATCAA CCGCGGTTTG GCCGGATCAA GATTCGCTTC TGGCAAGGTG
    CTATCCAGCT CTGGTATCTT TACGGGCCAC TACGCTCGAA CGCAGCCACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B