NCU04749 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU04749
Entrez Gene ID	3876429
Full Name	DNA replication helicase Dna2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396603.1	XP_011394905.1	DNA replication helicase Dna2
XM_011396604.1	XP_011394906.1	DNA replication helicase Dna2, variant

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU04749 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU04749 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf11356	XM_011396604.1 Latest version!	Neurospora crassa OR74A DNA replication helicase Dna2, variant (NCU04749), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ONf11355	XM_011396603.1 Latest version!	Neurospora crassa OR74A DNA replication helicase Dna2 (NCU04749), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU04749 ( XM_011396604.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394906.1 Neurospora crassa OR74A DNA replication helicase Dna2, variant (NCU04749), mRNA.

Price & Availability↑

CloneID	ONf11356
Clone ID Related Accession (Same CDS sequence)	XM_011396604.1
Accession Version	XM_011396604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA replication helicase Dna2, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506).

ATGTTTATGA AACCTAGTCA AAAGCCTAGA GCCATACAGA GGTATCTGGT AACCAATGTT 
CTCGAGAGCT CCTACACGGA TGACCGTGGC CGTGAATCCC CTGAGAAGAT CCTCATCGTA 
CAGGCTGATA GAACCAACTC TGTGCGAACG ATCCATCTTC GAGGCGACTG GTTTGATACT 
CCCGCAAGCG TCAGCGCATA CGTCCACATT ATCGGCCAGT TCGAAGACCA GGGAAAATGT 
GTCCTCGACA ACAACCAGAA TATGCTGATC CTGCACCCAG ATCAGCTCAT CTCATCCACT 
GTCGTTGCCG ACTCATTCAG CTGCATGCGG CGTGCGGTCT TGCAGGACAG AGTCAAAGCC 
ACCAGTGACT TGACACCCGC GCTTGTCTAC GGTACTATTC TGCACGAGAT TTTCCAGGAA 
GCTTTGGTAG CCAACAAATG GGATCCGGCA TTCCTCACCA ACGTCATCAA CAAGACTCTT 
GAGAAGCATC TTGAGGACCT GTACATCCTC AAGGTCAGCA TGGACGATGC AAAGAGTCAT 
GTGCAATCCA AGATGCCTGA ACTCCGGTCT TGGGCTCAGC TGTTCGTAAC TTCCCAGCCA 
AAGCCCGATG CTATCGTCCA AGGTCGGAAC GGTGACAAGG CTACCATGTG TGTCAGCAAG 
CTTCTGGATG TCGAGGAGCA TGTCTGGTCA CCCATGTATG GTCTCAAGGG CAACATTGAT 
GCCACCGTCC AGGTCACTAT GCGCGATCCC CCAGCAAGGG GCTCAAGCTC AAAGGCGCCA 
ACGACCAAGA CCTTGACCGT CCCCTTCGAG GTCAAGACCG GCAAGAATGT CAACAGCAAC 
CATCAGGCGC AAACAGCACT CTACAATTTG TTGCTCTCGG ATCGCTACGA CATTGAGATT 
GTCTACGGTA TCCTCTACTA CATGGAGACA TCGCAGACGT TGCGCATTCC CGCCGTTAGG 
CATGAAATCC GCCACATGAT CATGCAGCGC AACCAGCTGG CTTGCTACAT TCGTGAGCGG 
AGTGTCCAAC TGCCCCCTAT GAAGAAGAAC AAGAACGCGT GCGGCAGATG CTATGCCCAG 
ACGACCTGTT TCATCTATCA CAAGCTTGCT GATGACGGAG ATGGGGAGAC GAGCGCAATG 
AACGAGAAGT TTGATGAAGT AGTCCACCAT CTCAGCCCTA CCCACAAGGC GTTCTTCTTG 
AAATGGGAAG AACTTCTAAC CAAGGAGGAA AAGGAAAGCC AAAAGCTTCG CCGAGAGCTA 
TGGACTATGA TCAGCACAGA GCGCGAGAAG GTTGGACGTT GCTTCGCCAA TGTCATCATC 
GAGGAGGGCT CAGCATACGA GAATCACCAA CAGATGGGCA AGACCAACCG TTTCGCCTAC 
ACCTTTATCA AGGAAAAGCC TGCTCCCGGC TTTTCGTTTC TGGACTCGCA ACTGACTGTG 
GGTGAGCCCA TTGTCATTTC TGACGAACAA GGTCACTTTG CACTTGCGCT GGGCTATGTC 
ACCTCAGTCA GAAAGACTCG CATCGCTGTG GCCGTGGACA GAAGACTGCA CAATGCGCGC 
GTCCGGCAGC CTGGTTTCGA CGAGGCCGAC AATCAGACCT TTGCCAGCAT CATGCAGGTT 
CCTGGCTCTG GTGGTAACGC CCCGGAAGAC CGTGAAGCTG GCCCGGTCCG CTATCGTCTA 
GACAAAGATG AGTTCAGCAA TGGCATGGCA ACAGTACGCA ACAATCTTGT TCAGATCATG 
GCCGATGGAG TGCTTGGATC TAGGCAGATC AGGAGTTTGG TTGTCGACCT TGAGGCCCCC 
CGCTTCAAGG CTGTGCCAAC GCAGTATATT CTCCCTTCTA CCGCGTCGGG TCAAGGTCTG 
AACGTCGATC AGAAGGCGGC TGTTGAGAAG GTCATGTCTG CTCAGGACTA TGCCCTTGTG 
CTTGGTATGC CTGGTACGGG CAAGACGACT ACCATTGCGC ACATCATCCG TGCCTTGGTT 
GCACAGAAGA AAACAGTCTT GCTAACTTCC TACACCCACA CCGCCGTGGA CAACATCTTG 
TTGAAACTCA AGGATGACGG GATTCCGATT CTTCGTCTCG GTCCTCCCGT CAAGGTTCAC 
CCTGAGGTTC CTTCGTTCGC CACCCTCGCC GGCGTGCCAA AGACTTCTTT TTCGGAGATC 
CACGAGGCCT GGAACGACAC CCCGATTGTG GCGACGACTT GCTTGGGTAT CAACCACCCT 
GTCTTCGCCG AGCGCATCTT CGATTACTGC ATAGTCGACG AGGCTTCCCA GATCACGCTT 
CCTATCTGTC TCGGTCCCAT CCGTATGGCA CGCAAGTTTG TTCTAGTCGG TGACCATAAC 
CAGCTCCCTC CTCTCGTACA AAACGAGGAA GCTCGCCTTG GCGGTCTCGA CGTGAGCTTA 
TTTAAGCTGC TCTCCGAGAA ACACCCCCAA AGCGTAGTCA ATCTCGAACA CCAATACCGC 
ATGTGCGAAG ACATCATGAC CCTCTCCAAC ACGCTCATCT ACAATGGCCG GCTAAAATGC 
GGCACCGAAG CGTTACGCAC TACATCCCTC GAGATTCCCG ACATGGACGC TCTAGCAACC 
AAGCACTTTG ACGCCGATTC TTTCCTCGCC CACTCCCAGC AACACCCGCT CAAAGAAGGA 
GATAAACTCT CCTTCTGCCC CTCTCCCACC CCCACTACCT GCTGGCTCCG TGACCTTCTC 
GACCCCTCTG CGCGCGTTCG CTTCATCAAC ACCGACTCCC TCCTTCCGCA CTCACGCGAA 
GAGAAATCCA CTCGCGGTCA GCGCATCGTT AACCCGCTTG AGTCCCGCAT CGTCTGCCAG 
CTAGTCGACT CGCTCCTGAC CGTGGGCGTG CCCGCCTCTG AAATTGGTGT GATGACGCAT 
TACCGCTCGC AACTAGCTTT GCTTAAGCAC AATATGAAAA ACACCCTAGG CGGTAGCGCG 
GGAGCCATAA GAGCGGGCGT CAAAGTGGCG GCCGAGGAAA TCGAGATGCA TACCGCTGAT 
CGCTTCCAAG GAAGAGATAA GAGCGTTGTG GTGTTATCCC TGGTGCGAAG TAACGAGGGG 
TGTAGTATCG GCGAACTACT GAAAGATTGG AGGAGGATCA ACGTGGCGTT TACAAGGGCG 
AAGACAAAGC TGCTTGTTGT TGGTAGCCGT GAAACTTTGC GAGGGGCGTC TTCGTCGGTG 
GCTGGGTCTC CAACAGCAAT CGCTAGTCCC GAGCTGGGAG AAACCGGTGA TGATCAGGGA 
GAAGGGCAAG GAGAACCAGA AGGAGGCGAA ATGCTCGCCC GCTTTGTGCG CATGATGGAA 
GATCGCAACT GGGTGTATGA GTTGCGTCCT GGTGCTTTGG AGGGACATAG GTTTGCGGAT 
GCGAATGCTG CTTCCACTGC TGCTGATGCT CTCAGCCCTA GGAAACCTAA CCCTATCTTC 
AAGCCAGTTT CGCCTCGGAA GGGGTCGGCG TTGTCACCAT CGAAAAGGGC GAATGCGAAG 
CTTGCTGGGA AAGGATATAA GGTGCAGAGC AAGCTGCAGT TTGGGACACC AAGTCAGGGG 
AAAGAAAATG TCGGGAGCCA AAGCTTGAAG AGCCCGAAGA AGGGCATTGT TGGTAGAGGG 
AAACATAACC CCAAGCGCAT TGGAATTAGT GAGCGCGCAA TCATGAAGGG GAGGCCGATT 
ATGAGGGATA TCATGAATGA GATTACTGAT GGGAGGTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394906.1
CDS	1820..5521
Translation	MFMKPSQKPRAIQRYLVTNVLESSYTDDRGRESPEKILIVQADRTNSVRTIHLRGDWFDTPASVSAYVHIIGQFEDQGKCVLDNNQNMLILHPDQLISSTVVADSFSCMRRAVLQDRVKATSDLTPALVYGTILHEIFQEALVANKWDPAFLTNVINKTLEKHLEDLYILKVSMDDAKSHVQSKMPELRSWAQLFVTSQPKPDAIVQGRNGDKATMCVSKLLDVEEHVWSPMYGLKGNIDATVQVTMRDPPARGSSSKAPTTKTLTVPFEVKTGKNVNSNHQAQTALYNLLLSDRYDIEIVYGILYYMETSQTLRIPAVRHEIRHMIMQRNQLACYIRERSVQLPPMKKNKNACGRCYAQTTCFIYHKLADDGDGETSAMNEKFDEVVHHLSPTHKAFFLKWEELLTKEEKESQKLRRELWTMISTEREKVGRCFANVIIEEGSAYENHQQMGKTNRFAYTFIKEKPAPGFSFLDSQLTVGEPIVISDEQGHFALALGYVTSVRKTRIAVAVDRRLHNARVRQPGFDEADNQTFASIMQVPGSGGNAPEDREAGPVRYRLDKDEFSNGMATVRNNLVQIMADGVLGSRQIRSLVVDLEAPRFKAVPTQYILPSTASGQGLNVDQKAAVEKVMSAQDYALVLGMPGTGKTTTIAHIIRALVAQKKTVLLTSYTHTAVDNILLKLKDDGIPILRLGPPVKVHPEVPSFATLAGVPKTSFSEIHEAWNDTPIVATTCLGINHPVFAERIFDYCIVDEASQITLPICLGPIRMARKFVLVGDHNQLPPLVQNEEARLGGLDVSLFKLLSEKHPQSVVNLEHQYRMCEDIMTLSNTLIYNGRLKCGTEALRTTSLEIPDMDALATKHFDADSFLAHSQQHPLKEGDKLSFCPSPTPTTCWLRDLLDPSARVRFINTDSLLPHSREEKSTRGQRIVNPLESRIVCQLVDSLLTVGVPASEIGVMTHYRSQLALLKHNMKNTLGGSAGAIRAGVKVAAEEIEMHTADRFQGRDKSVVVLSLVRSNEGCSIGELLKDWRRINVAFTRAKTKLLVVGSRETLRGASSSVAGSPTAIASPELGETGDDQGEGQGEPEGGEMLARFVRMMEDRNWVYELRPGALEGHRFADANAASTAADALSPRKPNPIFKPVSPRKGSALSPSKRANAKLAGKGYKVQSKLQFGTPSQGKENVGSQSLKSPKKGIVGRGKHNPKRIGISERAIMKGRPIMRDIMNEITDGRF

Target ORF information:

RefSeq Version	XM_011396604.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA replication helicase Dna2, variant (NCU04749), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396604.1

ORF Insert Sequence:

ATGTTTATGA AACCTAGTCA AAAGCCTAGA GCCATACAGA GGTATCTGGT AACCAATGTT 
CTCGAGAGCT CCTACACGGA TGACCGTGGC CGTGAATCCC CTGAGAAGAT CCTCATCGTA 
CAGGCTGATA GAACCAACTC TGTGCGAACG ATCCATCTTC GAGGCGACTG GTTTGATACT 
CCCGCAAGCG TCAGCGCATA CGTCCACATT ATCGGCCAGT TCGAAGACCA GGGAAAATGT 
GTCCTCGACA ACAACCAGAA TATGCTGATC CTGCACCCAG ATCAGCTCAT CTCATCCACT 
GTCGTTGCCG ACTCATTCAG CTGCATGCGG CGTGCGGTCT TGCAGGACAG AGTCAAAGCC 
ACCAGTGACT TGACACCCGC GCTTGTCTAC GGTACTATTC TGCACGAGAT TTTCCAGGAA 
GCTTTGGTAG CCAACAAATG GGATCCGGCA TTCCTCACCA ACGTCATCAA CAAGACTCTT 
GAGAAGCATC TTGAGGACCT GTACATCCTC AAGGTCAGCA TGGACGATGC AAAGAGTCAT 
GTGCAATCCA AGATGCCTGA ACTCCGGTCT TGGGCTCAGC TGTTCGTAAC TTCCCAGCCA 
AAGCCCGATG CTATCGTCCA AGGTCGGAAC GGTGACAAGG CTACCATGTG TGTCAGCAAG 
CTTCTGGATG TCGAGGAGCA TGTCTGGTCA CCCATGTATG GTCTCAAGGG CAACATTGAT 
GCCACCGTCC AGGTCACTAT GCGCGATCCC CCAGCAAGGG GCTCAAGCTC AAAGGCGCCA 
ACGACCAAGA CCTTGACCGT CCCCTTCGAG GTCAAGACCG GCAAGAATGT CAACAGCAAC 
CATCAGGCGC AAACAGCACT CTACAATTTG TTGCTCTCGG ATCGCTACGA CATTGAGATT 
GTCTACGGTA TCCTCTACTA CATGGAGACA TCGCAGACGT TGCGCATTCC CGCCGTTAGG 
CATGAAATCC GCCACATGAT CATGCAGCGC AACCAGCTGG CTTGCTACAT TCGTGAGCGG 
AGTGTCCAAC TGCCCCCTAT GAAGAAGAAC AAGAACGCGT GCGGCAGATG CTATGCCCAG 
ACGACCTGTT TCATCTATCA CAAGCTTGCT GATGACGGAG ATGGGGAGAC GAGCGCAATG 
AACGAGAAGT TTGATGAAGT AGTCCACCAT CTCAGCCCTA CCCACAAGGC GTTCTTCTTG 
AAATGGGAAG AACTTCTAAC CAAGGAGGAA AAGGAAAGCC AAAAGCTTCG CCGAGAGCTA 
TGGACTATGA TCAGCACAGA GCGCGAGAAG GTTGGACGTT GCTTCGCCAA TGTCATCATC 
GAGGAGGGCT CAGCATACGA GAATCACCAA CAGATGGGCA AGACCAACCG TTTCGCCTAC 
ACCTTTATCA AGGAAAAGCC TGCTCCCGGC TTTTCGTTTC TGGACTCGCA ACTGACTGTG 
GGTGAGCCCA TTGTCATTTC TGACGAACAA GGTCACTTTG CACTTGCGCT GGGCTATGTC 
ACCTCAGTCA GAAAGACTCG CATCGCTGTG GCCGTGGACA GAAGACTGCA CAATGCGCGC 
GTCCGGCAGC CTGGTTTCGA CGAGGCCGAC AATCAGACCT TTGCCAGCAT CATGCAGGTT 
CCTGGCTCTG GTGGTAACGC CCCGGAAGAC CGTGAAGCTG GCCCGGTCCG CTATCGTCTA 
GACAAAGATG AGTTCAGCAA TGGCATGGCA ACAGTACGCA ACAATCTTGT TCAGATCATG 
GCCGATGGAG TGCTTGGATC TAGGCAGATC AGGAGTTTGG TTGTCGACCT TGAGGCCCCC 
CGCTTCAAGG CTGTGCCAAC GCAGTATATT CTCCCTTCTA CCGCGTCGGG TCAAGGTCTG 
AACGTCGATC AGAAGGCGGC TGTTGAGAAG GTCATGTCTG CTCAGGACTA TGCCCTTGTG 
CTTGGTATGC CTGGTACGGG CAAGACGACT ACCATTGCGC ACATCATCCG TGCCTTGGTT 
GCACAGAAGA AAACAGTCTT GCTAACTTCC TACACCCACA CCGCCGTGGA CAACATCTTG 
TTGAAACTCA AGGATGACGG GATTCCGATT CTTCGTCTCG GTCCTCCCGT CAAGGTTCAC 
CCTGAGGTTC CTTCGTTCGC CACCCTCGCC GGCGTGCCAA AGACTTCTTT TTCGGAGATC 
CACGAGGCCT GGAACGACAC CCCGATTGTG GCGACGACTT GCTTGGGTAT CAACCACCCT 
GTCTTCGCCG AGCGCATCTT CGATTACTGC ATAGTCGACG AGGCTTCCCA GATCACGCTT 
CCTATCTGTC TCGGTCCCAT CCGTATGGCA CGCAAGTTTG TTCTAGTCGG TGACCATAAC 
CAGCTCCCTC CTCTCGTACA AAACGAGGAA GCTCGCCTTG GCGGTCTCGA CGTGAGCTTA 
TTTAAGCTGC TCTCCGAGAA ACACCCCCAA AGCGTAGTCA ATCTCGAACA CCAATACCGC 
ATGTGCGAAG ACATCATGAC CCTCTCCAAC ACGCTCATCT ACAATGGCCG GCTAAAATGC 
GGCACCGAAG CGTTACGCAC TACATCCCTC GAGATTCCCG ACATGGACGC TCTAGCAACC 
AAGCACTTTG ACGCCGATTC TTTCCTCGCC CACTCCCAGC AACACCCGCT CAAAGAAGGA 
GATAAACTCT CCTTCTGCCC CTCTCCCACC CCCACTACCT GCTGGCTCCG TGACCTTCTC 
GACCCCTCTG CGCGCGTTCG CTTCATCAAC ACCGACTCCC TCCTTCCGCA CTCACGCGAA 
GAGAAATCCA CTCGCGGTCA GCGCATCGTT AACCCGCTTG AGTCCCGCAT CGTCTGCCAG 
CTAGTCGACT CGCTCCTGAC CGTGGGCGTG CCCGCCTCTG AAATTGGTGT GATGACGCAT 
TACCGCTCGC AACTAGCTTT GCTTAAGCAC AATATGAAAA ACACCCTAGG CGGTAGCGCG 
GGAGCCATAA GAGCGGGCGT CAAAGTGGCG GCCGAGGAAA TCGAGATGCA TACCGCTGAT 
CGCTTCCAAG GAAGAGATAA GAGCGTTGTG GTGTTATCCC TGGTGCGAAG TAACGAGGGG 
TGTAGTATCG GCGAACTACT GAAAGATTGG AGGAGGATCA ACGTGGCGTT TACAAGGGCG 
AAGACAAAGC TGCTTGTTGT TGGTAGCCGT GAAACTTTGC GAGGGGCGTC TTCGTCGGTG 
GCTGGGTCTC CAACAGCAAT CGCTAGTCCC GAGCTGGGAG AAACCGGTGA TGATCAGGGA 
GAAGGGCAAG GAGAACCAGA AGGAGGCGAA ATGCTCGCCC GCTTTGTGCG CATGATGGAA 
GATCGCAACT GGGTGTATGA GTTGCGTCCT GGTGCTTTGG AGGGACATAG GTTTGCGGAT 
GCGAATGCTG CTTCCACTGC TGCTGATGCT CTCAGCCCTA GGAAACCTAA CCCTATCTTC 
AAGCCAGTTT CGCCTCGGAA GGGGTCGGCG TTGTCACCAT CGAAAAGGGC GAATGCGAAG 
CTTGCTGGGA AAGGATATAA GGTGCAGAGC AAGCTGCAGT TTGGGACACC AAGTCAGGGG 
AAAGAAAATG TCGGGAGCCA AAGCTTGAAG AGCCCGAAGA AGGGCATTGT TGGTAGAGGG 
AAACATAACC CCAAGCGCAT TGGAATTAGT GAGCGCGCAA TCATGAAGGG GAGGCCGATT 
ATGAGGGATA TCATGAATGA GATTACTGAT GGGAGGTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU04749 ( XM_011396603.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394905.1 Neurospora crassa OR74A DNA replication helicase Dna2 (NCU04749), partial mRNA.

Price & Availability↑

CloneID	ONf11355
Clone ID Related Accession (Same CDS sequence)	XM_011396603.1
Accession Version	XM_011396603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA replication helicase Dna2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). COMPLETENESS: incomplete on the 5' end.

ATGCCTCTCA AAAAGTCTTA CTCGGACGCC TATGCTCAGC CCCCCAAGCG CGGAGGATGG 
CAGAGGACAA GAAGCAATCC CCCCAACAAA GGGCCTCTAC AATCGCTAGC CCCCGTCTCG 
GAATCTTCCA AGAACAAGCT CCAGGCCTTC CGATCCGACG ACACATCTTC CAAACCACCA 
CCAAAGTCTG CAAGGCCCGT AAAGGCCGCC ATCGACGAAC TGCAAAAAGA AAACATCAAC 
ACCACCAAGT CGATCCCCAG GAAGAGGCCA TTACCAGACT CCAACGACGA CGAGCCATCC 
AGCGACGCCA AAACTCCCGC AGATCGCCAT TCATGGAAAG ATTTGTTTGG TGTACCTGAA 
GTCATCACCG AAGAGGAAGA TGGAGAGAAA TCGCCCGGTG ACAGGGTCGA CTGGAGGTCC 
GGAGTGCCCT TACAACTACC CGCTTCGCCA ATGATTCCCA AGAATGGCAG GAGACGCGCG 
AAGAGCTCCT CGCCTGCTTC ATCACCGGCT ACCAAGTTGA CTACTCCCCA CGTCGCCCTC 
AAGAGGTTTG CCGGCGCTTC CAAGACACCC CACGCAGATC CTGCCTCGGA CTTGTGGAGC 
CGTTTCTCTT CTAATGGAAA TGATACGAGT CCCTCAAACC ACAAGAACCC CCTCTTTGCC 
CAGCTTATGG CATCTTCCTC ACCACGCCCG CAGTCAAGCG ATCGATCGTT GAGGAAGTCC 
GTCAGTTGCG GTTCCGCTTG GCCCAAGCGG CGCCGTACCC TTTGTCCTGA GGAGCAAAAT 
AGAATCGTTT CTCAGGACAC TCCAAGGAAG AAGAAGTCGA GTCTTTTGTC AGAACTACTA 
CAATCCGTTG ATGGAGAGAT CGACAGCTCG AAAACGCCAA GCAACGCTCC AGCACAGCAG 
AAGAAGTCAC CCTCTCCCAT GAAGAAGTCG CCATCAAAGA GAAAGATAAC ACCCCAAACC 
GTGCCTGACC AAAACTCCTC TCCCTCACGG AAAAAGGCCG AGCGGCAAAC TACCGAAACT 
GACGCCTCAA AGCAACCCGA AAAGGCATCT TCGGACTACG GTGATGACGA CTTTGACTTT 
GACGAGAGCA CTCTCCTCGA ACTGGACGCT AGTATCCTTG GTCAAGGCGA CGGTAGCACT 
CTCATAGCAA GTGACGCTGT CTCGCCAGCT CCACGCGAAT CGTCAAAGAC CCTTGTCGAT 
AATGACGAAT TTGGTGATCT CGATGACGAT ATTTTTGATG GGGTAGAAGA CCTGGTGGCT 
CAAGTTGAGT CTGCGACACA GAAACCTGCC CAGCGGTTGT CACCTAGAAA GAGACCGTCC 
CCCAAGAAGA GACTATCGCC TAGGAAGAGG CAGTCACCCA AGAAATTGCC CCAACAGGTT 
GCGGGCGATG AAGATGACGA GTTTGGTGAT GATTTCGGTA ATGATTTTGA CTTTGACGCG 
GTCGAAATGG CTGCGACACA ACGTGCCAGC CATGCGCAAG ATGTTTCCTC ACATCCTAGT 
CAAAAGCCTA GAGCCATACA GAGGTATCTG GTAACCAATG TTCTCGAGAG CTCCTACACG 
GATGACCGTG GCCGTGAATC CCCTGAGAAG ATCCTCATCG TACAGGCTGA TAGAACCAAC 
TCTGTGCGAA CGATCCATCT TCGAGGCGAC TGGTTTGATA CTCCCGCAAG CGTCAGCGCA 
TACGTCCACA TTATCGGCCA GTTCGAAGAC CAGGGAAAAT GTGTCCTCGA CAACAACCAG 
AATATGCTGA TCCTGCACCC AGATCAGCTC ATCTCATCCA CTGTCGTTGC CGACTCATTC 
AGCTGCATGC GGCGTGCGGT CTTGCAGGAC AGAGTCAAAG CCACCAGTGA CTTGACACCC 
GCGCTTGTCT ACGGTACTAT TCTGCACGAG ATTTTCCAGG AAGCTTTGGT AGCCAACAAA 
TGGGATCCGG CATTCCTCAC CAACGTCATC AACAAGACTC TTGAGAAGCA TCTTGAGGAC 
CTGTACATCC TCAAGGTCAG CATGGACGAT GCAAAGAGTC ATGTGCAATC CAAGATGCCT 
GAACTCCGGT CTTGGGCTCA GCTGTTCGTA ACTTCCCAGC CAAAGCCCGA TGCTATCGTC 
CAAGGTCGGA ACGGTGACAA GGCTACCATG TGTGTCAGCA AGCTTCTGGA TGTCGAGGAG 
CATGTCTGGT CACCCATGTA TGGTCTCAAG GGCAACATTG ATGCCACCGT CCAGGTCACT 
ATGCGCGATC CCCCAGCAAG GGGCTCAAGC TCAAAGGCGC CAACGACCAA GACCTTGACC 
GTCCCCTTCG AGGTCAAGAC CGGCAAGAAT GTCAACAGCA ACCATCAGGC GCAAACAGCA 
CTCTACAATT TGTTGCTCTC GGATCGCTAC GACATTGAGA TTGTCTACGG TATCCTCTAC 
TACATGGAGA CATCGCAGAC GTTGCGCATT CCCGCCGTTA GGCATGAAAT CCGCCACATG 
ATCATGCAGC GCAACCAGCT GGCTTGCTAC ATTCGTGAGC GGAGTGTCCA ACTGCCCCCT 
ATGAAGAAGA ACAAGAACGC GTGCGGCAGA TGCTATGCCC AGACGACCTG TTTCATCTAT 
CACAAGCTTG CTGATGACGG AGATGGGGAG ACGAGCGCAA TGAACGAGAA GTTTGATGAA 
GTAGTCCACC ATCTCAGCCC TACCCACAAG GCGTTCTTCT TGAAATGGGA AGAACTTCTA 
ACCAAGGAGG AAAAGGAAAG CCAAAAGCTT CGCCGAGAGC TATGGACTAT GATCAGCACA 
GAGCGCGAGA AGGTTGGACG TTGCTTCGCC AATGTCATCA TCGAGGAGGG CTCAGCATAC 
GAGAATCACC AACAGATGGG CAAGACCAAC CGTTTCGCCT ACACCTTTAT CAAGGAAAAG 
CCTGCTCCCG GCTTTTCGTT TCTGGACTCG CAACTGACTG TGGGTGAGCC CATTGTCATT 
TCTGACGAAC AAGGTCACTT TGCACTTGCG CTGGGCTATG TCACCTCAGT CAGAAAGACT 
CGCATCGCTG TGGCCGTGGA CAGAAGACTG CACAATGCGC GCGTCCGGCA GCCTGGTTTC 
GACGAGGCCG ACAATCAGAC CTTTGCCAGC ATCATGCAGG TTCCTGGCTC TGGTGGTAAC 
GCCCCGGAAG ACCGTGAAGC TGGCCCGGTC CGCTATCGTC TAGACAAAGA TGAGTTCAGC 
AATGGCATGG CAACAGTACG CAACAATCTT GTTCAGATCA TGGCCGATGG AGTGCTTGGA 
TCTAGGCAGA TCAGGAGTTT GGTTGTCGAC CTTGAGGCCC CCCGCTTCAA GGCTGTGCCA 
ACGCAGTATA TTCTCCCTTC TACCGCGTCG GGTCAAGGTC TGAACGTCGA TCAGAAGGCG 
GCTGTTGAGA AGGTCATGTC TGCTCAGGAC TATGCCCTTG TGCTTGGTAT GCCTGGTACG 
GGCAAGACGA CTACCATTGC GCACATCATC CGTGCCTTGG TTGCACAGAA GAAAACAGTC 
TTGCTAACTT CCTACACCCA CACCGCCGTG GACAACATCT TGTTGAAACT CAAGGATGAC 
GGGATTCCGA TTCTTCGTCT CGGTCCTCCC GTCAAGGTTC ACCCTGAGGT TCCTTCGTTC 
GCCACCCTCG CCGGCGTGCC AAAGACTTCT TTTTCGGAGA TCCACGAGGC CTGGAACGAC 
ACCCCGATTG TGGCGACGAC TTGCTTGGGT ATCAACCACC CTGTCTTCGC CGAGCGCATC 
TTCGATTACT GCATAGTCGA CGAGGCTTCC CAGATCACGC TTCCTATCTG TCTCGGTCCC 
ATCCGTATGG CACGCAAGTT TGTTCTAGTC GGTGACCATA ACCAGCTCCC TCCTCTCGTA 
CAAAACGAGG AAGCTCGCCT TGGCGGTCTC GACGTGAGCT TATTTAAGCT GCTCTCCGAG 
AAACACCCCC AAAGCGTAGT CAATCTCGAA CACCAATACC GCATGTGCGA AGACATCATG 
ACCCTCTCCA ACACGCTCAT CTACAATGGC CGGCTAAAAT GCGGCACCGA AGCGTTACGC 
ACTACATCCC TCGAGATTCC CGACATGGAC GCTCTAGCAA CCAAGCACTT TGACGCCGAT 
TCTTTCCTCG CCCACTCCCA GCAACACCCG CTCAAAGAAG GAGATAAACT CTCCTTCTGC 
CCCTCTCCCA CCCCCACTAC CTGCTGGCTC CGTGACCTTC TCGACCCCTC TGCGCGCGTT 
CGCTTCATCA ACACCGACTC CCTCCTTCCG CACTCACGCG AAGAGAAATC CACTCGCGGT 
CAGCGCATCG TTAACCCGCT TGAGTCCCGC ATCGTCTGCC AGCTAGTCGA CTCGCTCCTG 
ACCGTGGGCG TGCCCGCCTC TGAAATTGGT GTGATGACGC ATTACCGCTC GCAACTAGCT 
TTGCTTAAGC ACAATATGAA AAACACCCTA GGCGGTAGCG CGGGAGCCAT AAGAGCGGGC 
GTCAAAGTGG CGGCCGAGGA AATCGAGATG CATACCGCTG ATCGCTTCCA AGGAAGAGAT 
AAGAGCGTTG TGGTGTTATC CCTGGTGCGA AGTAACGAGG GGTGTAGTAT CGGCGAACTA 
CTGAAAGATT GGAGGAGGAT CAACGTGGCG TTTACAAGGG CGAAGACAAA GCTGCTTGTT 
GTTGGTAGCC GTGAAACTTT GCGAGGGGCG TCTTCGTCGG TGGCTGGGTC TCCAACAGCA 
ATCGCTAGTC CCGAGCTGGG AGAAACCGGT GATGATCAGG GAGAAGGGCA AGGAGAACCA 
GAAGGAGGCG AAATGCTCGC CCGCTTTGTG CGCATGATGG AAGATCGCAA CTGGGTGTAT 
GAGTTGCGTC CTGGTGCTTT GGAGGGACAT AGGTTTGCGG ATGCGAATGC TGCTTCCACT 
GCTGCTGATG CTCTCAGCCC TAGGAAACCT AACCCTATCT TCAAGCCAGT TTCGCCTCGG 
AAGGGGTCGG CGTTGTCACC ATCGAAAAGG GCGAATGCGA AGCTTGCTGG GAAAGGATAT 
AAGGTGCAGA GCAAGCTGCA GTTTGGGACA CCAAGTCAGG GGAAAGAAAA TGTCGGGAGC 
CAAAGCTTGA AGAGCCCGAA GAAGGGCATT GTTGGTAGAG GGAAACATAA CCCCAAGCGC 
ATTGGAATTA GTGAGCGCGC AATCATGAAG GGGAGGCCGA TTATGAGGGA TATCATGAAT 
GAGATTACTG ATGGGAGGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394905.1
CDS	1..5184
Translation	MPLKKSYSDAYAQPPKRGGWQRTRSNPPNKGPLQSLAPVSESSKNKLQAFRSDDTSSKPPPKSARPVKAAIDELQKENINTTKSIPRKRPLPDSNDDEPSSDAKTPADRHSWKDLFGVPEVITEEEDGEKSPGDRVDWRSGVPLQLPASPMIPKNGRRRAKSSSPASSPATKLTTPHVALKRFAGASKTPHADPASDLWSRFSSNGNDTSPSNHKNPLFAQLMASSSPRPQSSDRSLRKSVSCGSAWPKRRRTLCPEEQNRIVSQDTPRKKKSSLLSELLQSVDGEIDSSKTPSNAPAQQKKSPSPMKKSPSKRKITPQTVPDQNSSPSRKKAERQTTETDASKQPEKASSDYGDDDFDFDESTLLELDASILGQGDGSTLIASDAVSPAPRESSKTLVDNDEFGDLDDDIFDGVEDLVAQVESATQKPAQRLSPRKRPSPKKRLSPRKRQSPKKLPQQVAGDEDDEFGDDFGNDFDFDAVEMAATQRASHAQDVSSHPSQKPRAIQRYLVTNVLESSYTDDRGRESPEKILIVQADRTNSVRTIHLRGDWFDTPASVSAYVHIIGQFEDQGKCVLDNNQNMLILHPDQLISSTVVADSFSCMRRAVLQDRVKATSDLTPALVYGTILHEIFQEALVANKWDPAFLTNVINKTLEKHLEDLYILKVSMDDAKSHVQSKMPELRSWAQLFVTSQPKPDAIVQGRNGDKATMCVSKLLDVEEHVWSPMYGLKGNIDATVQVTMRDPPARGSSSKAPTTKTLTVPFEVKTGKNVNSNHQAQTALYNLLLSDRYDIEIVYGILYYMETSQTLRIPAVRHEIRHMIMQRNQLACYIRERSVQLPPMKKNKNACGRCYAQTTCFIYHKLADDGDGETSAMNEKFDEVVHHLSPTHKAFFLKWEELLTKEEKESQKLRRELWTMISTEREKVGRCFANVIIEEGSAYENHQQMGKTNRFAYTFIKEKPAPGFSFLDSQLTVGEPIVISDEQGHFALALGYVTSVRKTRIAVAVDRRLHNARVRQPGFDEADNQTFASIMQVPGSGGNAPEDREAGPVRYRLDKDEFSNGMATVRNNLVQIMADGVLGSRQIRSLVVDLEAPRFKAVPTQYILPSTASGQGLNVDQKAAVEKVMSAQDYALVLGMPGTGKTTTIAHIIRALVAQKKTVLLTSYTHTAVDNILLKLKDDGIPILRLGPPVKVHPEVPSFATLAGVPKTSFSEIHEAWNDTPIVATTCLGINHPVFAERIFDYCIVDEASQITLPICLGPIRMARKFVLVGDHNQLPPLVQNEEARLGGLDVSLFKLLSEKHPQSVVNLEHQYRMCEDIMTLSNTLIYNGRLKCGTEALRTTSLEIPDMDALATKHFDADSFLAHSQQHPLKEGDKLSFCPSPTPTTCWLRDLLDPSARVRFINTDSLLPHSREEKSTRGQRIVNPLESRIVCQLVDSLLTVGVPASEIGVMTHYRSQLALLKHNMKNTLGGSAGAIRAGVKVAAEEIEMHTADRFQGRDKSVVVLSLVRSNEGCSIGELLKDWRRINVAFTRAKTKLLVVGSRETLRGASSSVAGSPTAIASPELGETGDDQGEGQGEPEGGEMLARFVRMMEDRNWVYELRPGALEGHRFADANAASTAADALSPRKPNPIFKPVSPRKGSALSPSKRANAKLAGKGYKVQSKLQFGTPSQGKENVGSQSLKSPKKGIVGRGKHNPKRIGISERAIMKGRPIMRDIMNEITDGRF

Target ORF information:

RefSeq Version	XM_011396603.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA replication helicase Dna2 (NCU04749), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396603.1

ORF Insert Sequence:

ATGCCTCTCA AAAAGTCTTA CTCGGACGCC TATGCTCAGC CCCCCAAGCG CGGAGGATGG 
CAGAGGACAA GAAGCAATCC CCCCAACAAA GGGCCTCTAC AATCGCTAGC CCCCGTCTCG 
GAATCTTCCA AGAACAAGCT CCAGGCCTTC CGATCCGACG ACACATCTTC CAAACCACCA 
CCAAAGTCTG CAAGGCCCGT AAAGGCCGCC ATCGACGAAC TGCAAAAAGA AAACATCAAC 
ACCACCAAGT CGATCCCCAG GAAGAGGCCA TTACCAGACT CCAACGACGA CGAGCCATCC 
AGCGACGCCA AAACTCCCGC AGATCGCCAT TCATGGAAAG ATTTGTTTGG TGTACCTGAA 
GTCATCACCG AAGAGGAAGA TGGAGAGAAA TCGCCCGGTG ACAGGGTCGA CTGGAGGTCC 
GGAGTGCCCT TACAACTACC CGCTTCGCCA ATGATTCCCA AGAATGGCAG GAGACGCGCG 
AAGAGCTCCT CGCCTGCTTC ATCACCGGCT ACCAAGTTGA CTACTCCCCA CGTCGCCCTC 
AAGAGGTTTG CCGGCGCTTC CAAGACACCC CACGCAGATC CTGCCTCGGA CTTGTGGAGC 
CGTTTCTCTT CTAATGGAAA TGATACGAGT CCCTCAAACC ACAAGAACCC CCTCTTTGCC 
CAGCTTATGG CATCTTCCTC ACCACGCCCG CAGTCAAGCG ATCGATCGTT GAGGAAGTCC 
GTCAGTTGCG GTTCCGCTTG GCCCAAGCGG CGCCGTACCC TTTGTCCTGA GGAGCAAAAT 
AGAATCGTTT CTCAGGACAC TCCAAGGAAG AAGAAGTCGA GTCTTTTGTC AGAACTACTA 
CAATCCGTTG ATGGAGAGAT CGACAGCTCG AAAACGCCAA GCAACGCTCC AGCACAGCAG 
AAGAAGTCAC CCTCTCCCAT GAAGAAGTCG CCATCAAAGA GAAAGATAAC ACCCCAAACC 
GTGCCTGACC AAAACTCCTC TCCCTCACGG AAAAAGGCCG AGCGGCAAAC TACCGAAACT 
GACGCCTCAA AGCAACCCGA AAAGGCATCT TCGGACTACG GTGATGACGA CTTTGACTTT 
GACGAGAGCA CTCTCCTCGA ACTGGACGCT AGTATCCTTG GTCAAGGCGA CGGTAGCACT 
CTCATAGCAA GTGACGCTGT CTCGCCAGCT CCACGCGAAT CGTCAAAGAC CCTTGTCGAT 
AATGACGAAT TTGGTGATCT CGATGACGAT ATTTTTGATG GGGTAGAAGA CCTGGTGGCT 
CAAGTTGAGT CTGCGACACA GAAACCTGCC CAGCGGTTGT CACCTAGAAA GAGACCGTCC 
CCCAAGAAGA GACTATCGCC TAGGAAGAGG CAGTCACCCA AGAAATTGCC CCAACAGGTT 
GCGGGCGATG AAGATGACGA GTTTGGTGAT GATTTCGGTA ATGATTTTGA CTTTGACGCG 
GTCGAAATGG CTGCGACACA ACGTGCCAGC CATGCGCAAG ATGTTTCCTC ACATCCTAGT 
CAAAAGCCTA GAGCCATACA GAGGTATCTG GTAACCAATG TTCTCGAGAG CTCCTACACG 
GATGACCGTG GCCGTGAATC CCCTGAGAAG ATCCTCATCG TACAGGCTGA TAGAACCAAC 
TCTGTGCGAA CGATCCATCT TCGAGGCGAC TGGTTTGATA CTCCCGCAAG CGTCAGCGCA 
TACGTCCACA TTATCGGCCA GTTCGAAGAC CAGGGAAAAT GTGTCCTCGA CAACAACCAG 
AATATGCTGA TCCTGCACCC AGATCAGCTC ATCTCATCCA CTGTCGTTGC CGACTCATTC 
AGCTGCATGC GGCGTGCGGT CTTGCAGGAC AGAGTCAAAG CCACCAGTGA CTTGACACCC 
GCGCTTGTCT ACGGTACTAT TCTGCACGAG ATTTTCCAGG AAGCTTTGGT AGCCAACAAA 
TGGGATCCGG CATTCCTCAC CAACGTCATC AACAAGACTC TTGAGAAGCA TCTTGAGGAC 
CTGTACATCC TCAAGGTCAG CATGGACGAT GCAAAGAGTC ATGTGCAATC CAAGATGCCT 
GAACTCCGGT CTTGGGCTCA GCTGTTCGTA ACTTCCCAGC CAAAGCCCGA TGCTATCGTC 
CAAGGTCGGA ACGGTGACAA GGCTACCATG TGTGTCAGCA AGCTTCTGGA TGTCGAGGAG 
CATGTCTGGT CACCCATGTA TGGTCTCAAG GGCAACATTG ATGCCACCGT CCAGGTCACT 
ATGCGCGATC CCCCAGCAAG GGGCTCAAGC TCAAAGGCGC CAACGACCAA GACCTTGACC 
GTCCCCTTCG AGGTCAAGAC CGGCAAGAAT GTCAACAGCA ACCATCAGGC GCAAACAGCA 
CTCTACAATT TGTTGCTCTC GGATCGCTAC GACATTGAGA TTGTCTACGG TATCCTCTAC 
TACATGGAGA CATCGCAGAC GTTGCGCATT CCCGCCGTTA GGCATGAAAT CCGCCACATG 
ATCATGCAGC GCAACCAGCT GGCTTGCTAC ATTCGTGAGC GGAGTGTCCA ACTGCCCCCT 
ATGAAGAAGA ACAAGAACGC GTGCGGCAGA TGCTATGCCC AGACGACCTG TTTCATCTAT 
CACAAGCTTG CTGATGACGG AGATGGGGAG ACGAGCGCAA TGAACGAGAA GTTTGATGAA 
GTAGTCCACC ATCTCAGCCC TACCCACAAG GCGTTCTTCT TGAAATGGGA AGAACTTCTA 
ACCAAGGAGG AAAAGGAAAG CCAAAAGCTT CGCCGAGAGC TATGGACTAT GATCAGCACA 
GAGCGCGAGA AGGTTGGACG TTGCTTCGCC AATGTCATCA TCGAGGAGGG CTCAGCATAC 
GAGAATCACC AACAGATGGG CAAGACCAAC CGTTTCGCCT ACACCTTTAT CAAGGAAAAG 
CCTGCTCCCG GCTTTTCGTT TCTGGACTCG CAACTGACTG TGGGTGAGCC CATTGTCATT 
TCTGACGAAC AAGGTCACTT TGCACTTGCG CTGGGCTATG TCACCTCAGT CAGAAAGACT 
CGCATCGCTG TGGCCGTGGA CAGAAGACTG CACAATGCGC GCGTCCGGCA GCCTGGTTTC 
GACGAGGCCG ACAATCAGAC CTTTGCCAGC ATCATGCAGG TTCCTGGCTC TGGTGGTAAC 
GCCCCGGAAG ACCGTGAAGC TGGCCCGGTC CGCTATCGTC TAGACAAAGA TGAGTTCAGC 
AATGGCATGG CAACAGTACG CAACAATCTT GTTCAGATCA TGGCCGATGG AGTGCTTGGA 
TCTAGGCAGA TCAGGAGTTT GGTTGTCGAC CTTGAGGCCC CCCGCTTCAA GGCTGTGCCA 
ACGCAGTATA TTCTCCCTTC TACCGCGTCG GGTCAAGGTC TGAACGTCGA TCAGAAGGCG 
GCTGTTGAGA AGGTCATGTC TGCTCAGGAC TATGCCCTTG TGCTTGGTAT GCCTGGTACG 
GGCAAGACGA CTACCATTGC GCACATCATC CGTGCCTTGG TTGCACAGAA GAAAACAGTC 
TTGCTAACTT CCTACACCCA CACCGCCGTG GACAACATCT TGTTGAAACT CAAGGATGAC 
GGGATTCCGA TTCTTCGTCT CGGTCCTCCC GTCAAGGTTC ACCCTGAGGT TCCTTCGTTC 
GCCACCCTCG CCGGCGTGCC AAAGACTTCT TTTTCGGAGA TCCACGAGGC CTGGAACGAC 
ACCCCGATTG TGGCGACGAC TTGCTTGGGT ATCAACCACC CTGTCTTCGC CGAGCGCATC 
TTCGATTACT GCATAGTCGA CGAGGCTTCC CAGATCACGC TTCCTATCTG TCTCGGTCCC 
ATCCGTATGG CACGCAAGTT TGTTCTAGTC GGTGACCATA ACCAGCTCCC TCCTCTCGTA 
CAAAACGAGG AAGCTCGCCT TGGCGGTCTC GACGTGAGCT TATTTAAGCT GCTCTCCGAG 
AAACACCCCC AAAGCGTAGT CAATCTCGAA CACCAATACC GCATGTGCGA AGACATCATG 
ACCCTCTCCA ACACGCTCAT CTACAATGGC CGGCTAAAAT GCGGCACCGA AGCGTTACGC 
ACTACATCCC TCGAGATTCC CGACATGGAC GCTCTAGCAA CCAAGCACTT TGACGCCGAT 
TCTTTCCTCG CCCACTCCCA GCAACACCCG CTCAAAGAAG GAGATAAACT CTCCTTCTGC 
CCCTCTCCCA CCCCCACTAC CTGCTGGCTC CGTGACCTTC TCGACCCCTC TGCGCGCGTT 
CGCTTCATCA ACACCGACTC CCTCCTTCCG CACTCACGCG AAGAGAAATC CACTCGCGGT 
CAGCGCATCG TTAACCCGCT TGAGTCCCGC ATCGTCTGCC AGCTAGTCGA CTCGCTCCTG 
ACCGTGGGCG TGCCCGCCTC TGAAATTGGT GTGATGACGC ATTACCGCTC GCAACTAGCT 
TTGCTTAAGC ACAATATGAA AAACACCCTA GGCGGTAGCG CGGGAGCCAT AAGAGCGGGC 
GTCAAAGTGG CGGCCGAGGA AATCGAGATG CATACCGCTG ATCGCTTCCA AGGAAGAGAT 
AAGAGCGTTG TGGTGTTATC CCTGGTGCGA AGTAACGAGG GGTGTAGTAT CGGCGAACTA 
CTGAAAGATT GGAGGAGGAT CAACGTGGCG TTTACAAGGG CGAAGACAAA GCTGCTTGTT 
GTTGGTAGCC GTGAAACTTT GCGAGGGGCG TCTTCGTCGG TGGCTGGGTC TCCAACAGCA 
ATCGCTAGTC CCGAGCTGGG AGAAACCGGT GATGATCAGG GAGAAGGGCA AGGAGAACCA 
GAAGGAGGCG AAATGCTCGC CCGCTTTGTG CGCATGATGG AAGATCGCAA CTGGGTGTAT 
GAGTTGCGTC CTGGTGCTTT GGAGGGACAT AGGTTTGCGG ATGCGAATGC TGCTTCCACT 
GCTGCTGATG CTCTCAGCCC TAGGAAACCT AACCCTATCT TCAAGCCAGT TTCGCCTCGG 
AAGGGGTCGG CGTTGTCACC ATCGAAAAGG GCGAATGCGA AGCTTGCTGG GAAAGGATAT 
AAGGTGCAGA GCAAGCTGCA GTTTGGGACA CCAAGTCAGG GGAAAGAAAA TGTCGGGAGC 
CAAAGCTTGA AGAGCCCGAA GAAGGGCATT GTTGGTAGAG GGAAACATAA CCCCAAGCGC 
ATTGGAATTA GTGAGCGCGC AATCATGAAG GGGAGGCCGA TTATGAGGGA TATCATGAAT 
GAGATTACTG ATGGGAGGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU04749 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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