NCU03986 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03986
Entrez Gene ID	3872718
Full Name	DNA-directed RNA polymerase III largest subunit
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_951478.3	XP_956571.3	DNA-directed RNA polymerase III largest subunit

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR3A	NP_008986.2
	POLR3A	XP_002805710.1
Mus musculus (house mouse)	Polr3a	NP_001074716.1
Gallus gallus (chicken)	POLR3A	NP_001006500.1
Danio rerio (zebrafish)	polr3a	NP_001263425.1
Bos taurus (cattle)	POLR3A	NP_001077219.1
Caenorhabditis elegans (roundworm)	rpc-1	NP_501127.2
Rattus norvegicus (Norway rat)	Polr3a	XP_341389.5
Drosophila melanogaster (fruit fly)	CG17209	NP_001096987.2
Magnaporthe oryzae (rice blast fungus)	MGG_04477	XP_003710983.1
Arabidopsis thaliana (thale cress)	NRPC1	NP_200812.2
Oryza sativa (rice)	Os04g0492300	NP_001053175.1
Saccharomyces cerevisiae (baker's yeast)	RPO31	NP_014759.1
Kluyveromyces lactis	KLLA0E21253g	XP_454912.1
Eremothecium gossypii	AGOS_AER252C	NP_985109.2
Schizosaccharomyces pombe (fission yeast)	rpc1	NP_595506.1
Xenopus tropicalis (tropical clawed frog)	polr3a	NP_001096178.1
Pan troglodytes (chimpanzee)	POLR3A	XP_507865.4
Canis lupus familiaris (dog)	POLR3A	XP_536399.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004703	XP_318130.4
Neurospora crassa	NCU03986	XP_956571.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03986 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03986 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10103	XM_951478.3 Latest version!	Neurospora crassa OR74A DNA-directed RNA polymerase III largest subunit (NCU03986), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03986 ( XM_951478.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956571.3 Neurospora crassa OR74A DNA-directed RNA polymerase III largest subunit (NCU03986), mRNA.

Price & Availability↑

CloneID	ONf10103
Clone ID Related Accession (Same CDS sequence)	XM_951478.3
Accession Version	XM_951478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA-directed RNA polymerase III largest subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_951478.2.

ATGGGTGCCG AGCAGAGAGC TCCTATCAAG GAGCAGCTGG CCGACCAGCT CCCTAAGCGC 
TTCAGAGCCC TGCAGTTCGG AGTCCAGTCA CACCAAGATA TAGTAAATCA GGGTGTCCTC 
GAAGTCTCCG ACAACCTCCT TTACGATGTC GAAAACGGCC GCCGCACTTT CCCCCACGGC 
CCCCTCGATC CCCGTCTCGG TACTTCGAGC AAGACGGGCA CCTGCGAGAC TTGCAACCTC 
CCTCTCCAGT CCTGCGCCGG TCACTTCGGT CACGTCCGCC TCCCACTTCC CGTCTTCCAC 
ATCGGCTATC TCAAGTACAT TCAGGCCACG CTCCAAAACA TTTGCAAGAC CTGCTCAAAG 
GTCCTGCTCG AGGAGGAACC CAAGGCCAAG TTCCTCAAGG CTCTCCGTGC CCCTGGTATC 
GATAACCTCA AGCGATCGGC CATCCTCAAG AAGATCAACG ATGAATGCCG CAAGCAAAAG 
ACGTGCCCCT ACTGCGGCGA CACCCAGGGT GTTATTCGCA AGTTGAGCGT CATGAAGCTT 
ACCCACGACA AGTTCTCGCA CTTCAACAAG TCGACTGCCC AGAAAAAGGT TCCGCCCGTT 
GCCAAGGCCG CCTTCGATTC CTCCTTCATC CAGGCAAAAA CATTCACTCC GGACCTCGAG 
AAGCACTCCA AGAAAGCTAT GGACGACATG GATCCTCTCA AGGTCCTAAA ATTGTTCAAG 
AAGATTACCG CCGAGGACTG CGAGTTGCTC GGATTGAACC CTTCCGAGGG CAGACCAGAG 
ATGTTCTTGT GGCAATATCT TCCCGCTCCC CCAATCTGTA TTCGTCCGTC GGTCCAGCAA 
GAAGCCGCCA GTAACGAGGA TGACATTACT AGTAAACTCT CCGAAATCAT TCTCTACGCC 
GGCCATTTGC GCGAATCCCT CCTCAAAGGT GCTGCCCTCA ACACCATCAT GGAACAGTGG 
GAGTTCATCC AGCTCCAGGT TGGTATGTAC GTCAACAGTG ATGTTCCCGG TCTCTACCAG 
CCCGGCTTTG GCAAGCCCAT CCGCGGCTTC TGCCAGCGTC TGAAGGGTAA GCAGGGTCGT 
TTCCGTGGTA ATCTGTCGGG TAAGCGTGTC GACTTCTCCG GCCGCACAGT CATTTCGCCT 
GATCCCAACT TGGGAATCGA CCAGGTTGCC GTGCCCGAGC GCGTGGCCAA GAACCTGACC 
TATCCCGAGC GGGTGAACCG CAACAACATT GAAAAGTTGC GAAAATGCGT CATGAATGGA 
CCCAAAGTAT GGCCCGGTGC TCAGGGCGTC ATCAAAAGGG ACGGCGGCAT GAAGTTCAAC 
CTTAACTTTG GAGATGAGTC CATCCGGGAG GAACGCGCGC GGGAACTCAA GTTTGGTGAC 
GTTGTTGAAA GACATCTGGA AGACGGCGAC ATTGTACTCT TCAACCGTCA GCCCTCGCTA 
CACAAGTTGT CTATCATGAG TCATCGTGCA AAGGTTAGAC CCTGGAGGAC GTTCCGTCTC 
AACGAATGCG TGTGCACTCC CTATAACGCC GATTTCGATG GAGACGAAAT GAACCTTCAT 
CTTCCGCAAA CAGAAGAGGC TCGCGCCGAA GCCATCAACC TCATGGGTGT CCAAAACAAC 
TTGATCACCC CCAAGAACGG TGAACCTATT ATCGCCGCCA CGCAGGATTT CATTACTGCG 
GCATACCTCC TGAGTAGCAA AGATTTGTTC CTCGACCGCA AGACCTTTAC CTACATCTGC 
ACGCAGATGC TGTTGGGCGA AACCCATCTC GACCTTCCCC CGCCTACCAT CATCAAACCC 
GTCGAGTTGT GGACGGGCAA GCAGATTTTC AACGTTTTGA TGCGTCCCAA CAAGCAATCA 
CCGGTCCTCG TCAACCTCGA GGCCAAGAAC AAGGTCTACA AGGCGAAGCC AGGCGAGCTT 
CCGGATATGG ACATTGATGA TTGCTACTTG GTCGTCCGCA ACTCCGAGGT CATGTGCGGA 
AGAATGGACA AGTCCACCGT TGGTGGTGGC AAGAAGAACT CGGTCTTTTA TGTTATCCTT 
CGTGATTGGG GTCCGGAGCA CGCTGCTCAG GCCATGAACC GTCTGGCCAA GTTGTGTGCT 
CGCACACTAA CCCTCCGCGG GTTTTCGATT GGTGTTGGCG ATGTCTATCC ATCCAAGGAT 
TTGACGGAGC ACAAGGCTAA GCTGGTTCAG GATGCGTATG CACTCTGCGA CGACCTCATC 
GCACAGTTCA AGTCTGGAAG GCTTGAAAAG GCTCCCGGTT GTAACATGGA GGAGACGCTT 
GAGAACAAGA TCTCTGGTAT TCTCAGTAAA GTCCGTTCGC AGGCCGGTGA CTACTGTGTC 
GGCCATTTGA GTCGCAACAA CTCTCCCTTG ATCATGGCCA AATCCGGTTC CAAGGGTTCC 
GACATCAACG TCGCTCAGAT GGTTGCCCTT GTCGGACAGC AGATTATTGG TGGCAAGCGT 
GTTGCGGATG GATTCCAGGA TCGTACCCTC TTCCACTTCC ACAAGAACGC TCGCCAGCCC 
CCCAGCAAGG GTTTCGTGCG CAACAGTTTC TACACTGGTC TGGTGCCCAC TGAGTTCCTT 
TTCCACGCCA TCTCTGGCAG AGAAGGTTTG GTCGATACCG CCGTCAAGAC AGCCGAGACA 
GGTTACATGT CTCGTCGTCT CATGAAGTCG TTGGAAGATC TCTCAACTCA GTACGACGAT 
ACCGTGCGGA CATCGGAAGG AAATATTGTG CAGTTCCAGT ACGGTGCCGA CAGACTGGAT 
CCAGCAGACA TGGAGGGTAA CGCCAAGCCT GTTGATTTCG AGAGATCATG GAATCATGCC 
GAAAGCTTGA CAATGAACCA CAATGACAGG TCGCTGCTTC CGGCAGAAAT CTCGGCCATG 
TGCAAGGAGA TTCTGGACGA AAGGCGGAAA TGGTACGTGC GCAAGCACCT TGTCACGGGC 
GAGTTGCTGC ACTACGAGGA CAAGACGATT GCGGAAAGTG ACGATCACGA TGTGTTCATT 
GCCATCGATG AGCATGAGGG TGCCAGAGTC TATCTCAACA CCATTCAGGC TGAGATGGAC 
AAGCGCGCAC TCAAGCTGGC AAAAGCACGC AAACTCGCTG GCTTGGATCC TTGTCTGCCC 
CAGGACAACG CGGACCTGCT GAAGGAATTT GCTGCATGGG AGAAGAAGCA AAACGGCGGC 
GATATCAAGA TGTTGGATCC GGAAGAAGAA GAAATCCAGC AAGCCCACGT TGATCGTGTC 
GCCAAGGTTT CGGAGGCTAC CCTGAGAAAG TTCATGAACC TCTGTCTTTC CAAGTTCGAT 
AAGGCAAAGG TGGAGCCTGG CCACGCCGTC GGCGCCGTTG GTGCGCAATC TATCGGTGAA 
CCCGGTACGC AGATGACACT CAAGACTTTC CATTTCGCCG GTGTCGCTGG TATGAGTATC 
ACTCAGGGTG TTCCCCGTAT CAAGGAAATC ATTGGCGCCT CGAAACTCAT CAGCACGCCA 
GTCATCAGCT GCGAGCTCGA GAACAACCGA GACATCAAGG CCGCGCGCAT CGTCAAGGGC 
AAGATTGAAA AGACTTATAT CTCGGATGTC ATCACTTATG TTGAGGATGT CTGGCTTCCG 
GATGTTGCCA AGATTGTCCT CAAGATCGAT ATGCAGGCTC TTTCGGACAT GCAGCTCGGT 
ATCGGTCTTC GCGAGATCAT GGTGGCCATC GTCAAAGCTA AGAAGCTCAA GCTAAAGGTG 
GAGATGGACG ATCTCAAGAT CGGTCCCGAT AATACTATTG AGGTCATCGT GAGCAACGAC 
TGGAAGGATC TGACCGCACA AAAGAAGGCG GCGCGAGTTC GCGCCGCAGC GATTGAGAAG 
GGCCAATTGC TTCTCACCAC CTCGGACGAG ACCGCCGCCG ACTTCCAACT GCGCGTCAAC 
TTCCTCAAGC GCATGCTCCC TGCTGTGCCC ATCTCCGGCT ACCCCGAGGC TTTCCGTGCC 
ATCATCCAAT CCAGCGAGTC CAATGAGCAC AAGGTCCTCG TTGAAGGTTA CGGCCTGCGC 
GCCTGCATGA CCACGGAGGG CGTCATCGGC ACCAAGTGTA TCTCCAACTC CATCATGGAG 
TGCCGCGACG TTTTGGGCAT CGAAGCCGCC CGCACCACCA TCGCCAAGGA AATCGGCAGC 
GTCATGGGCG ACATGGGCAT CGATCCCCGT CACATGGATT TGCTGGCCGA CGTCATGACT 
TACAAGGGCG AGATTCTCGG TATCACGCGC CATGGTCTCG CCAAGATGCG CGACAGCGTT 
CTGCAGCTTG CCTCATTCGA GCGCACGCCC GATCACTTGT TCGATGCGGC CGCGGGCATG 
AAGACCGACC GCATTCTCGG TGTGTCGGAG TGTATCATCA TGGGCCAGAC CATGAAGATC 
GGTACGGGAG CATTCCAGGT CGTCAGACGG TTGGGTATCC GGGATCATCA GGTTAGACCC 
AAAAAGTCCA TGTTCGAGGA GGTGTGGAAG AAGGATGAGG CAGAAAAGAG GAGGGCAAAG 
ATGGAGATGA GGAAGGCCAC TGCGGCTGCG GCTGCGTCTA CCGGGCGTGG GGGAAAGACT 
GATGGTATGA TGCCGGCAGC GCCCATGGTG GCTGTTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956571.3
CDS	591..5192
Translation	MGAEQRAPIKEQLADQLPKRFRALQFGVQSHQDIVNQGVLEVSDNLLYDVENGRRTFPHGPLDPRLGTSSKTGTCETCNLPLQSCAGHFGHVRLPLPVFHIGYLKYIQATLQNICKTCSKVLLEEEPKAKFLKALRAPGIDNLKRSAILKKINDECRKQKTCPYCGDTQGVIRKLSVMKLTHDKFSHFNKSTAQKKVPPVAKAAFDSSFIQAKTFTPDLEKHSKKAMDDMDPLKVLKLFKKITAEDCELLGLNPSEGRPEMFLWQYLPAPPICIRPSVQQEAASNEDDITSKLSEIILYAGHLRESLLKGAALNTIMEQWEFIQLQVGMYVNSDVPGLYQPGFGKPIRGFCQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLGIDQVAVPERVAKNLTYPERVNRNNIEKLRKCVMNGPKVWPGAQGVIKRDGGMKFNLNFGDESIREERARELKFGDVVERHLEDGDIVLFNRQPSLHKLSIMSHRAKVRPWRTFRLNECVCTPYNADFDGDEMNLHLPQTEEARAEAINLMGVQNNLITPKNGEPIIAATQDFITAAYLLSSKDLFLDRKTFTYICTQMLLGETHLDLPPPTIIKPVELWTGKQIFNVLMRPNKQSPVLVNLEAKNKVYKAKPGELPDMDIDDCYLVVRNSEVMCGRMDKSTVGGGKKNSVFYVILRDWGPEHAAQAMNRLAKLCARTLTLRGFSIGVGDVYPSKDLTEHKAKLVQDAYALCDDLIAQFKSGRLEKAPGCNMEETLENKISGILSKVRSQAGDYCVGHLSRNNSPLIMAKSGSKGSDINVAQMVALVGQQIIGGKRVADGFQDRTLFHFHKNARQPPSKGFVRNSFYTGLVPTEFLFHAISGREGLVDTAVKTAETGYMSRRLMKSLEDLSTQYDDTVRTSEGNIVQFQYGADRLDPADMEGNAKPVDFERSWNHAESLTMNHNDRSLLPAEISAMCKEILDERRKWYVRKHLVTGELLHYEDKTIAESDDHDVFIAIDEHEGARVYLNTIQAEMDKRALKLAKARKLAGLDPCLPQDNADLLKEFAAWEKKQNGGDIKMLDPEEEEIQQAHVDRVAKVSEATLRKFMNLCLSKFDKAKVEPGHAVGAVGAQSIGEPGTQMTLKTFHFAGVAGMSITQGVPRIKEIIGASKLISTPVISCELENNRDIKAARIVKGKIEKTYISDVITYVEDVWLPDVAKIVLKIDMQALSDMQLGIGLREIMVAIVKAKKLKLKVEMDDLKIGPDNTIEVIVSNDWKDLTAQKKAARVRAAAIEKGQLLLTTSDETAADFQLRVNFLKRMLPAVPISGYPEAFRAIIQSSESNEHKVLVEGYGLRACMTTEGVIGTKCISNSIMECRDVLGIEAARTTIAKEIGSVMGDMGIDPRHMDLLADVMTYKGEILGITRHGLAKMRDSVLQLASFERTPDHLFDAAAGMKTDRILGVSECIIMGQTMKIGTGAFQVVRRLGIRDHQVRPKKSMFEEVWKKDEAEKRRAKMEMRKATAAAAASTGRGGKTDGMMPAAPMVAVA

Target ORF information:

RefSeq Version	XM_951478.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA-directed RNA polymerase III largest subunit (NCU03986), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_951478.3

ORF Insert Sequence:

ATGGGTGCCG AGCAGAGAGC TCCTATCAAG GAGCAGCTGG CCGACCAGCT CCCTAAGCGC 
TTCAGAGCCC TGCAGTTCGG AGTCCAGTCA CACCAAGATA TAGTAAATCA GGGTGTCCTC 
GAAGTCTCCG ACAACCTCCT TTACGATGTC GAAAACGGCC GCCGCACTTT CCCCCACGGC 
CCCCTCGATC CCCGTCTCGG TACTTCGAGC AAGACGGGCA CCTGCGAGAC TTGCAACCTC 
CCTCTCCAGT CCTGCGCCGG TCACTTCGGT CACGTCCGCC TCCCACTTCC CGTCTTCCAC 
ATCGGCTATC TCAAGTACAT TCAGGCCACG CTCCAAAACA TTTGCAAGAC CTGCTCAAAG 
GTCCTGCTCG AGGAGGAACC CAAGGCCAAG TTCCTCAAGG CTCTCCGTGC CCCTGGTATC 
GATAACCTCA AGCGATCGGC CATCCTCAAG AAGATCAACG ATGAATGCCG CAAGCAAAAG 
ACGTGCCCCT ACTGCGGCGA CACCCAGGGT GTTATTCGCA AGTTGAGCGT CATGAAGCTT 
ACCCACGACA AGTTCTCGCA CTTCAACAAG TCGACTGCCC AGAAAAAGGT TCCGCCCGTT 
GCCAAGGCCG CCTTCGATTC CTCCTTCATC CAGGCAAAAA CATTCACTCC GGACCTCGAG 
AAGCACTCCA AGAAAGCTAT GGACGACATG GATCCTCTCA AGGTCCTAAA ATTGTTCAAG 
AAGATTACCG CCGAGGACTG CGAGTTGCTC GGATTGAACC CTTCCGAGGG CAGACCAGAG 
ATGTTCTTGT GGCAATATCT TCCCGCTCCC CCAATCTGTA TTCGTCCGTC GGTCCAGCAA 
GAAGCCGCCA GTAACGAGGA TGACATTACT AGTAAACTCT CCGAAATCAT TCTCTACGCC 
GGCCATTTGC GCGAATCCCT CCTCAAAGGT GCTGCCCTCA ACACCATCAT GGAACAGTGG 
GAGTTCATCC AGCTCCAGGT TGGTATGTAC GTCAACAGTG ATGTTCCCGG TCTCTACCAG 
CCCGGCTTTG GCAAGCCCAT CCGCGGCTTC TGCCAGCGTC TGAAGGGTAA GCAGGGTCGT 
TTCCGTGGTA ATCTGTCGGG TAAGCGTGTC GACTTCTCCG GCCGCACAGT CATTTCGCCT 
GATCCCAACT TGGGAATCGA CCAGGTTGCC GTGCCCGAGC GCGTGGCCAA GAACCTGACC 
TATCCCGAGC GGGTGAACCG CAACAACATT GAAAAGTTGC GAAAATGCGT CATGAATGGA 
CCCAAAGTAT GGCCCGGTGC TCAGGGCGTC ATCAAAAGGG ACGGCGGCAT GAAGTTCAAC 
CTTAACTTTG GAGATGAGTC CATCCGGGAG GAACGCGCGC GGGAACTCAA GTTTGGTGAC 
GTTGTTGAAA GACATCTGGA AGACGGCGAC ATTGTACTCT TCAACCGTCA GCCCTCGCTA 
CACAAGTTGT CTATCATGAG TCATCGTGCA AAGGTTAGAC CCTGGAGGAC GTTCCGTCTC 
AACGAATGCG TGTGCACTCC CTATAACGCC GATTTCGATG GAGACGAAAT GAACCTTCAT 
CTTCCGCAAA CAGAAGAGGC TCGCGCCGAA GCCATCAACC TCATGGGTGT CCAAAACAAC 
TTGATCACCC CCAAGAACGG TGAACCTATT ATCGCCGCCA CGCAGGATTT CATTACTGCG 
GCATACCTCC TGAGTAGCAA AGATTTGTTC CTCGACCGCA AGACCTTTAC CTACATCTGC 
ACGCAGATGC TGTTGGGCGA AACCCATCTC GACCTTCCCC CGCCTACCAT CATCAAACCC 
GTCGAGTTGT GGACGGGCAA GCAGATTTTC AACGTTTTGA TGCGTCCCAA CAAGCAATCA 
CCGGTCCTCG TCAACCTCGA GGCCAAGAAC AAGGTCTACA AGGCGAAGCC AGGCGAGCTT 
CCGGATATGG ACATTGATGA TTGCTACTTG GTCGTCCGCA ACTCCGAGGT CATGTGCGGA 
AGAATGGACA AGTCCACCGT TGGTGGTGGC AAGAAGAACT CGGTCTTTTA TGTTATCCTT 
CGTGATTGGG GTCCGGAGCA CGCTGCTCAG GCCATGAACC GTCTGGCCAA GTTGTGTGCT 
CGCACACTAA CCCTCCGCGG GTTTTCGATT GGTGTTGGCG ATGTCTATCC ATCCAAGGAT 
TTGACGGAGC ACAAGGCTAA GCTGGTTCAG GATGCGTATG CACTCTGCGA CGACCTCATC 
GCACAGTTCA AGTCTGGAAG GCTTGAAAAG GCTCCCGGTT GTAACATGGA GGAGACGCTT 
GAGAACAAGA TCTCTGGTAT TCTCAGTAAA GTCCGTTCGC AGGCCGGTGA CTACTGTGTC 
GGCCATTTGA GTCGCAACAA CTCTCCCTTG ATCATGGCCA AATCCGGTTC CAAGGGTTCC 
GACATCAACG TCGCTCAGAT GGTTGCCCTT GTCGGACAGC AGATTATTGG TGGCAAGCGT 
GTTGCGGATG GATTCCAGGA TCGTACCCTC TTCCACTTCC ACAAGAACGC TCGCCAGCCC 
CCCAGCAAGG GTTTCGTGCG CAACAGTTTC TACACTGGTC TGGTGCCCAC TGAGTTCCTT 
TTCCACGCCA TCTCTGGCAG AGAAGGTTTG GTCGATACCG CCGTCAAGAC AGCCGAGACA 
GGTTACATGT CTCGTCGTCT CATGAAGTCG TTGGAAGATC TCTCAACTCA GTACGACGAT 
ACCGTGCGGA CATCGGAAGG AAATATTGTG CAGTTCCAGT ACGGTGCCGA CAGACTGGAT 
CCAGCAGACA TGGAGGGTAA CGCCAAGCCT GTTGATTTCG AGAGATCATG GAATCATGCC 
GAAAGCTTGA CAATGAACCA CAATGACAGG TCGCTGCTTC CGGCAGAAAT CTCGGCCATG 
TGCAAGGAGA TTCTGGACGA AAGGCGGAAA TGGTACGTGC GCAAGCACCT TGTCACGGGC 
GAGTTGCTGC ACTACGAGGA CAAGACGATT GCGGAAAGTG ACGATCACGA TGTGTTCATT 
GCCATCGATG AGCATGAGGG TGCCAGAGTC TATCTCAACA CCATTCAGGC TGAGATGGAC 
AAGCGCGCAC TCAAGCTGGC AAAAGCACGC AAACTCGCTG GCTTGGATCC TTGTCTGCCC 
CAGGACAACG CGGACCTGCT GAAGGAATTT GCTGCATGGG AGAAGAAGCA AAACGGCGGC 
GATATCAAGA TGTTGGATCC GGAAGAAGAA GAAATCCAGC AAGCCCACGT TGATCGTGTC 
GCCAAGGTTT CGGAGGCTAC CCTGAGAAAG TTCATGAACC TCTGTCTTTC CAAGTTCGAT 
AAGGCAAAGG TGGAGCCTGG CCACGCCGTC GGCGCCGTTG GTGCGCAATC TATCGGTGAA 
CCCGGTACGC AGATGACACT CAAGACTTTC CATTTCGCCG GTGTCGCTGG TATGAGTATC 
ACTCAGGGTG TTCCCCGTAT CAAGGAAATC ATTGGCGCCT CGAAACTCAT CAGCACGCCA 
GTCATCAGCT GCGAGCTCGA GAACAACCGA GACATCAAGG CCGCGCGCAT CGTCAAGGGC 
AAGATTGAAA AGACTTATAT CTCGGATGTC ATCACTTATG TTGAGGATGT CTGGCTTCCG 
GATGTTGCCA AGATTGTCCT CAAGATCGAT ATGCAGGCTC TTTCGGACAT GCAGCTCGGT 
ATCGGTCTTC GCGAGATCAT GGTGGCCATC GTCAAAGCTA AGAAGCTCAA GCTAAAGGTG 
GAGATGGACG ATCTCAAGAT CGGTCCCGAT AATACTATTG AGGTCATCGT GAGCAACGAC 
TGGAAGGATC TGACCGCACA AAAGAAGGCG GCGCGAGTTC GCGCCGCAGC GATTGAGAAG 
GGCCAATTGC TTCTCACCAC CTCGGACGAG ACCGCCGCCG ACTTCCAACT GCGCGTCAAC 
TTCCTCAAGC GCATGCTCCC TGCTGTGCCC ATCTCCGGCT ACCCCGAGGC TTTCCGTGCC 
ATCATCCAAT CCAGCGAGTC CAATGAGCAC AAGGTCCTCG TTGAAGGTTA CGGCCTGCGC 
GCCTGCATGA CCACGGAGGG CGTCATCGGC ACCAAGTGTA TCTCCAACTC CATCATGGAG 
TGCCGCGACG TTTTGGGCAT CGAAGCCGCC CGCACCACCA TCGCCAAGGA AATCGGCAGC 
GTCATGGGCG ACATGGGCAT CGATCCCCGT CACATGGATT TGCTGGCCGA CGTCATGACT 
TACAAGGGCG AGATTCTCGG TATCACGCGC CATGGTCTCG CCAAGATGCG CGACAGCGTT 
CTGCAGCTTG CCTCATTCGA GCGCACGCCC GATCACTTGT TCGATGCGGC CGCGGGCATG 
AAGACCGACC GCATTCTCGG TGTGTCGGAG TGTATCATCA TGGGCCAGAC CATGAAGATC 
GGTACGGGAG CATTCCAGGT CGTCAGACGG TTGGGTATCC GGGATCATCA GGTTAGACCC 
AAAAAGTCCA TGTTCGAGGA GGTGTGGAAG AAGGATGAGG CAGAAAAGAG GAGGGCAAAG 
ATGGAGATGA GGAAGGCCAC TGCGGCTGCG GCTGCGTCTA CCGGGCGTGG GGGAAAGACT 
GATGGTATGA TGCCGGCAGC GCCCATGGTG GCTGTTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03986 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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