Polh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polh
Entrez Gene ID	80905
Full Name	polymerase (DNA directed), eta (RAD 30 related)
Synonyms	RAD30A,XPV
General protein information	Preferred Names polymerase (DNA directed), eta (RAD 30 related) Names DNA polymerase eta DNA-directed DNA polymerase eta RAD30 homolog A xeroderma pigmentosum variant type protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_030715.3	NP_109640.1	DNA polymerase eta
NM_030715.4	NP_109640.1	DNA polymerase eta isoform 1
XM_006525176.2	XP_006525239.1	DNA polymerase eta isoform X1
NM_001364947.1	NP_001351876.1	DNA polymerase eta isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLH	NP_006493.1
	POLH	XP_002803805.1
Canis lupus familiaris (dog)	POLH	XP_532150.2
Rattus norvegicus (Norway rat)	Polh	NP_001101674.1
Danio rerio (zebrafish)	polh	NP_001035337.1
Xenopus tropicalis (tropical clawed frog)	polh	XP_002942198.2
Mus musculus (house mouse)	Polh	NP_109640.1
Bos taurus (cattle)	POLH	NP_001029622.1
Gallus gallus (chicken)	POLH	NP_001001304.1
Pan troglodytes (chimpanzee)	POLH	XP_518497.1
Caenorhabditis elegans (roundworm)	polh-1	NP_497480.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
Zhou L, Dai H, Wu J, Zhou M, Yuan H, Du J, Yang L, Wu X, Xu H, Hua Y, Xu J, Zheng L, Shen B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)

A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro.
Fritzen R, Delbos F, De Smet A, Palancade B, Canman CE, Aoufouchi S, Weill JC, Reynaud CA, Storck S
DNA repair4637-46(2016 10)

The Polymerase Activity of Mammalian DNA Pol ? Is Specifically Required for Cell and Embryonic Viability.
Lange SS, Tomida J, Boulware KS, Bhetawal S, Wood RD
PLoS genetics12(1)e1005759(2016 Jan)

Ablation of XP-V gene causes adipose tissue senescence and metabolic abnormalities.
Chen YW, Harris RA, Hatahet Z, Chou KM
Proceedings of the National Academy of Sciences of the United States of America112(33)E4556-64(2015 Aug)

UV-induced mutations in epidermal cells of mice defective in DNA polymerase ? and/or ?.
Kanao R, Yokoi M, Ohkumo T, Sakurai Y, Dotsu K, Kura S, Nakatsu Y, Tsuzuki T, Masutani C, Hanaoka F
DNA repair29139-46(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the functions of REV3L in maintaining cell viability, embryonic viability and genomic stability are directly dependent on its polymerase activity, and cannot be ameliorated by an additional deletion of pol eta.
Title: The Polymerase Activity of Mammalian DNA Pol ? Is Specifically Required for Cell and Embryonic Viability.

XP-V has a role in adipose tissue senescence and metabolic syndrome
Title: Ablation of XP-V gene causes adipose tissue senescence and metabolic abnormalities.

Increase in UV-induced mutations at both G:C and A:T pairs associated with PolH deficiency suggests that PolH contributes to accurate translesion synthesis (TLS) past both T- & C-containing dimers.
Title: UV-induced mutations in epidermal cells of mice defective in DNA polymerase ? and/or ?.

POLH deficiency did not affect the frequency and patterns of C:G mutations and UNG POLH double deficiency showed an additive effect of single deficiency.
Title: Analysis of Ig gene hypermutation in Ung(-/-)Polh(-/-) mice suggests that UNG and A:T mutagenesis pathway target different U:G lesions.

REV1 and Polkappa are involved in DNA damage tolerance via Poleta-REV1 interaction when Poleta fails to bypass its cognate substrates.
Title: Stalled Pol? at its cognate substrate initiates an alternative translesion synthesis pathway via interaction with REV1.

The following Polh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54208	NM_001364947.1 Latest version!	Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu54208	XM_006525176.2 Latest version!	Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu08412	NM_030715.4 Latest version!	Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OMu08412	NM_030715.3	Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polh ( NM_001364947.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351876.1 Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu54208
Clone ID Related Accession (Same CDS sequence)	XM_006525176.2 , NM_001364947.1
Accession Version	NM_001364947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	DNA polymerase eta isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ048830.1, BC128366.1 and AC116739.13. On Jul 17, 2018 this sequence version replaced XM_006525176.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC128366.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCCG AGCATTTGGT GTCACTAGAA ACATGTGGGC GGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTCCTGGCA CAAGTTCGTG AGTCCCGTGG AAAAGCTAAC CTCACCAAAG 
GCTATTCGGA AACAAGGCTT GCTTCAGTGG CTTGATTCTC TTCAGAGTGA TGATCCCACC 
TCTCCAGACC TCAGGCTGAC TGTGGGAGCC ATGATTGTGG AAGAAATGAG AGCAGCCATA 
GAGAGTAAGA CTGGCTTCCA GTGTTCAGCT GGAATCTCAC ACAATAAGGT CCTGGCAAAA 
CTTGCTTGTG GACTAAATAA GCCCAACCGC CAAACCCTGG TCTCACATGG GTCAGTGCCA 
CAGCTCTTCA GCCAGATGCC AATCCGTAAA ATCCGAAGTC TTGGAGGAAA GCTAGGGGCT 
TCTGTCATTG AAGTCCTTGG GATAGAATAC ATGGGTGACC TGACCCAGTT CACTGAATCC 
CAGCTCCAGA GTCATTTTGG AGAGAAGAAT GGGTCTTGGC TGTATGCCAT GTGTCGAGGT 
ATTGAACACG ATCCAGTTAA ACCCAGGCAG TTACCTAAAA CTATTGGCTG CAGCAAGAAC 
TTCCCAGGGA AAACAGCTCT GGCTACTCGG GAGCAGGTAC AGTGGTGGCT TTTGCAGTTA 
GCTCTGGAAC TTGAGGAGAG GCTAACCAAA GATCGAAATG ATAATGACCG CGTGGCCACT 
CAGCTGGTCG TCAGTATTCG TTTTCAGGGA GACAGGCGTC TCAGCAGCCT GCGGCGGTGC 
TGTGCCCTCC CTCGCTATGA CGCTCACAAG ATGAGCCAGG ACGCATTTGC TGCCATCAGG 
AACTGTAATA CATCTGGAAT CCAAACTGAG TGGTCCCCTC CTCTCACAAT GCTCTTCCTC 
TGCGCTACCA AGTTCTCTGC GGCTGCCCCT CCAGCTTGCA CGGATATCAC TGCGTTCCTG 
AGTAGCGACT CAAGTTGTCA GCCAAAGGTG CCAATTGCCA GCTCTGAGAC TAGGACCCAG 
GGGTCTGGCC CAGCTGTACC AACCTCTAAA GAGGCAGCCA CATCTCTAGC GTCTTTTTTT 
CAAAAAGCTG CCAAAAAGCA GAGAATGAAA GAGACTTCCT TTGTACCTCT TAACACAGCC 
ACAGAGAAGT TATCATCCAA GCCTTCACTT GTGTTCCAAA GCAGCCAAAC CACAGGAAGT 
CAGTCTTTCT TTAAGCAGAA GAGCCTGCTG CTCCAGCACA CACAGCTTAG TAACTCTGCG 
GCTCCTGACC CTCCACAGGC CAGCCCTGCA GCGCAACCAA GCTGTCTGCC AGCAGAGTGT 
GTAGACAGTG GTCCTGATGA TGGAGCGGTG AAGCCAGTAT CCTCTAAAGC AGTGTCTACA 
GAAATGAATG TGGCTGGGGA CAGTCCAAAC GTGCTCGATT CTCCAGCTTA CAACTCTCAG 
GAGGTGACCC AAAGGGCCAC TGAGGACCAA GTGCTCTGTG AGAAGTGTGA CTCCCTGGTT 
CCAGTGTGGG ACATGCCAGA ACACACGGAC TACCATTTTG CATTGGAGTT GCAGAAGTCT 
TTTTTGCAGC CTTGTACTTC AAAACCCCAG GCTATTCCTG CCGTGTCTCC TCAGGGCAAA 
AGAAATCCCA AGAGTCCCTC GGCTTCCAGT AGTAAGCGCC TTAGGCCCCA TGGCATGCAG 
ACGCTGGAAT CGTTTTTTAA GCCACTGACA CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351876.1
CDS	406..2121
Translation	MKPEHLVSLETCGRMMLRSYVQIFSWHKFVSPVEKLTSPKAIRKQGLLQWLDSLQSDDPTSPDLRLTVGAMIVEEMRAAIESKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEVLGIEYMGDLTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLALELEERLTKDRNDNDRVATQLVVSIRFQGDRRLSSLRRCCALPRYDAHKMSQDAFAAIRNCNTSGIQTEWSPPLTMLFLCATKFSAAAPPACTDITAFLSSDSSCQPKVPIASSETRTQGSGPAVPTSKEAATSLASFFQKAAKKQRMKETSFVPLNTATEKLSSKPSLVFQSSQTTGSQSFFKQKSLLLQHTQLSNSAAPDPPQASPAAQPSCLPAECVDSGPDDGAVKPVSSKAVSTEMNVAGDSPNVLDSPAYNSQEVTQRATEDQVLCEKCDSLVPVWDMPEHTDYHFALELQKSFLQPCTSKPQAIPAVSPQGKRNPKSPSASSSKRLRPHGMQTLESFFKPLTH

Target ORF information:

RefSeq Version	NM_001364947.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364947.1

ORF Insert Sequence:

ATGAAGCCCG AGCATTTGGT GTCACTAGAA ACATGTGGGC GGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTCCTGGCA CAAGTTCGTG AGTCCCGTGG AAAAGCTAAC CTCACCAAAG 
GCTATTCGGA AACAAGGCTT GCTTCAGTGG CTTGATTCTC TTCAGAGTGA TGATCCCACC 
TCTCCAGACC TCAGGCTGAC TGTGGGAGCC ATGATTGTGG AAGAAATGAG AGCAGCCATA 
GAGAGTAAGA CTGGCTTCCA GTGTTCAGCT GGAATCTCAC ACAATAAGGT CCTGGCAAAA 
CTTGCTTGTG GACTAAATAA GCCCAACCGC CAAACCCTGG TCTCACATGG GTCAGTGCCA 
CAGCTCTTCA GCCAGATGCC AATCCGTAAA ATCCGAAGTC TTGGAGGAAA GCTAGGGGCT 
TCTGTCATTG AAGTCCTTGG GATAGAATAC ATGGGTGACC TGACCCAGTT CACTGAATCC 
CAGCTCCAGA GTCATTTTGG AGAGAAGAAT GGGTCTTGGC TGTATGCCAT GTGTCGAGGT 
ATTGAACACG ATCCAGTTAA ACCCAGGCAG TTACCTAAAA CTATTGGCTG CAGCAAGAAC 
TTCCCAGGGA AAACAGCTCT GGCTACTCGG GAGCAGGTAC AGTGGTGGCT TTTGCAGTTA 
GCTCTGGAAC TTGAGGAGAG GCTAACCAAA GATCGAAATG ATAATGACCG CGTGGCCACT 
CAGCTGGTCG TCAGTATTCG TTTTCAGGGA GACAGGCGTC TCAGCAGCCT GCGGCGGTGC 
TGTGCCCTCC CTCGCTATGA CGCTCACAAG ATGAGCCAGG ACGCATTTGC TGCCATCAGG 
AACTGTAATA CATCTGGAAT CCAAACTGAG TGGTCCCCTC CTCTCACAAT GCTCTTCCTC 
TGCGCTACCA AGTTCTCTGC GGCTGCCCCT CCAGCTTGCA CGGATATCAC TGCGTTCCTG 
AGTAGCGACT CAAGTTGTCA GCCAAAGGTG CCAATTGCCA GCTCTGAGAC TAGGACCCAG 
GGGTCTGGCC CAGCTGTACC AACCTCTAAA GAGGCAGCCA CATCTCTAGC GTCTTTTTTT 
CAAAAAGCTG CCAAAAAGCA GAGAATGAAA GAGACTTCCT TTGTACCTCT TAACACAGCC 
ACAGAGAAGT TATCATCCAA GCCTTCACTT GTGTTCCAAA GCAGCCAAAC CACAGGAAGT 
CAGTCTTTCT TTAAGCAGAA GAGCCTGCTG CTCCAGCACA CACAGCTTAG TAACTCTGCG 
GCTCCTGACC CTCCACAGGC CAGCCCTGCA GCGCAACCAA GCTGTCTGCC AGCAGAGTGT 
GTAGACAGTG GTCCTGATGA TGGAGCGGTG AAGCCAGTAT CCTCTAAAGC AGTGTCTACA 
GAAATGAATG TGGCTGGGGA CAGTCCAAAC GTGCTCGATT CTCCAGCTTA CAACTCTCAG 
GAGGTGACCC AAAGGGCCAC TGAGGACCAA GTGCTCTGTG AGAAGTGTGA CTCCCTGGTT 
CCAGTGTGGG ACATGCCAGA ACACACGGAC TACCATTTTG CATTGGAGTT GCAGAAGTCT 
TTTTTGCAGC CTTGTACTTC AAAACCCCAG GCTATTCCTG CCGTGTCTCC TCAGGGCAAA 
AGAAATCCCA AGAGTCCCTC GGCTTCCAGT AGTAAGCGCC TTAGGCCCCA TGGCATGCAG 
ACGCTGGAAT CGTTTTTTAA GCCACTGACA CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polh ( XM_006525176.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525239.1 Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54208
Clone ID Related Accession (Same CDS sequence)	XM_006525176.2 , NM_001364947.1
Accession Version	XM_006525176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA polymerase eta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCG AGCATTTGGT GTCACTAGAA ACATGTGGGC GGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTCCTGGCA CAAGTTCGTG AGTCCCGTGG AAAAGCTAAC CTCACCAAAG 
GCTATTCGGA AACAAGGCTT GCTTCAGTGG CTTGATTCTC TTCAGAGTGA TGATCCCACC 
TCTCCAGACC TCAGGCTGAC TGTGGGAGCC ATGATTGTGG AAGAAATGAG AGCAGCCATA 
GAGAGTAAGA CTGGCTTCCA GTGTTCAGCT GGAATCTCAC ACAATAAGGT CCTGGCAAAA 
CTTGCTTGTG GACTAAATAA GCCCAACCGC CAAACCCTGG TCTCACATGG GTCAGTGCCA 
CAGCTCTTCA GCCAGATGCC AATCCGTAAA ATCCGAAGTC TTGGAGGAAA GCTAGGGGCT 
TCTGTCATTG AAGTCCTTGG GATAGAATAC ATGGGTGACC TGACCCAGTT CACTGAATCC 
CAGCTCCAGA GTCATTTTGG AGAGAAGAAT GGGTCTTGGC TGTATGCCAT GTGTCGAGGT 
ATTGAACACG ATCCAGTTAA ACCCAGGCAG TTACCTAAAA CTATTGGCTG CAGCAAGAAC 
TTCCCAGGGA AAACAGCTCT GGCTACTCGG GAGCAGGTAC AGTGGTGGCT TTTGCAGTTA 
GCTCTGGAAC TTGAGGAGAG GCTAACCAAA GATCGAAATG ATAATGACCG CGTGGCCACT 
CAGCTGGTCG TCAGTATTCG TTTTCAGGGA GACAGGCGTC TCAGCAGCCT GCGGCGGTGC 
TGTGCCCTCC CTCGCTATGA CGCTCACAAG ATGAGCCAGG ACGCATTTGC TGCCATCAGG 
AACTGTAATA CATCTGGAAT CCAAACTGAG TGGTCCCCTC CTCTCACAAT GCTCTTCCTC 
TGCGCTACCA AGTTCTCTGC GGCTGCCCCT CCAGCTTGCA CGGATATCAC TGCGTTCCTG 
AGTAGCGACT CAAGTTGTCA GCCAAAGGTG CCAATTGCCA GCTCTGAGAC TAGGACCCAG 
GGGTCTGGCC CAGCTGTACC AACCTCTAAA GAGGCAGCCA CATCTCTAGC GTCTTTTTTT 
CAAAAAGCTG CCAAAAAGCA GAGAATGAAA GAGACTTCCT TTGTACCTCT TAACACAGCC 
ACAGAGAAGT TATCATCCAA GCCTTCACTT GTGTTCCAAA GCAGCCAAAC CACAGGAAGT 
CAGTCTTTCT TTAAGCAGAA GAGCCTGCTG CTCCAGCACA CACAGCTTAG TAACTCTGCG 
GCTCCTGACC CTCCACAGGC CAGCCCTGCA GCGCAACCAA GCTGTCTGCC AGCAGAGTGT 
GTAGACAGTG GTCCTGATGA TGGAGCGGTG AAGCCAGTAT CCTCTAAAGC AGTGTCTACA 
GAAATGAATG TGGCTGGGGA CAGTCCAAAC GTGCTCGATT CTCCAGCTTA CAACTCTCAG 
GAGGTGACCC AAAGGGCCAC TGAGGACCAA GTGCTCTGTG AGAAGTGTGA CTCCCTGGTT 
CCAGTGTGGG ACATGCCAGA ACACACGGAC TACCATTTTG CATTGGAGTT GCAGAAGTCT 
TTTTTGCAGC CTTGTACTTC AAAACCCCAG GCTATTCCTG CCGTGTCTCC TCAGGGCAAA 
AGAAATCCCA AGAGTCCCTC GGCTTCCAGT AGTAAGCGCC TTAGGCCCCA TGGCATGCAG 
ACGCTGGAAT CGTTTTTTAA GCCACTGACA CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525239.1
CDS	447..2162
Translation	MKPEHLVSLETCGRMMLRSYVQIFSWHKFVSPVEKLTSPKAIRKQGLLQWLDSLQSDDPTSPDLRLTVGAMIVEEMRAAIESKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEVLGIEYMGDLTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLALELEERLTKDRNDNDRVATQLVVSIRFQGDRRLSSLRRCCALPRYDAHKMSQDAFAAIRNCNTSGIQTEWSPPLTMLFLCATKFSAAAPPACTDITAFLSSDSSCQPKVPIASSETRTQGSGPAVPTSKEAATSLASFFQKAAKKQRMKETSFVPLNTATEKLSSKPSLVFQSSQTTGSQSFFKQKSLLLQHTQLSNSAAPDPPQASPAAQPSCLPAECVDSGPDDGAVKPVSSKAVSTEMNVAGDSPNVLDSPAYNSQEVTQRATEDQVLCEKCDSLVPVWDMPEHTDYHFALELQKSFLQPCTSKPQAIPAVSPQGKRNPKSPSASSSKRLRPHGMQTLESFFKPLTH

Target ORF information:

RefSeq Version	XM_006525176.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525176.2

ORF Insert Sequence:

ATGAAGCCCG AGCATTTGGT GTCACTAGAA ACATGTGGGC GGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTCCTGGCA CAAGTTCGTG AGTCCCGTGG AAAAGCTAAC CTCACCAAAG 
GCTATTCGGA AACAAGGCTT GCTTCAGTGG CTTGATTCTC TTCAGAGTGA TGATCCCACC 
TCTCCAGACC TCAGGCTGAC TGTGGGAGCC ATGATTGTGG AAGAAATGAG AGCAGCCATA 
GAGAGTAAGA CTGGCTTCCA GTGTTCAGCT GGAATCTCAC ACAATAAGGT CCTGGCAAAA 
CTTGCTTGTG GACTAAATAA GCCCAACCGC CAAACCCTGG TCTCACATGG GTCAGTGCCA 
CAGCTCTTCA GCCAGATGCC AATCCGTAAA ATCCGAAGTC TTGGAGGAAA GCTAGGGGCT 
TCTGTCATTG AAGTCCTTGG GATAGAATAC ATGGGTGACC TGACCCAGTT CACTGAATCC 
CAGCTCCAGA GTCATTTTGG AGAGAAGAAT GGGTCTTGGC TGTATGCCAT GTGTCGAGGT 
ATTGAACACG ATCCAGTTAA ACCCAGGCAG TTACCTAAAA CTATTGGCTG CAGCAAGAAC 
TTCCCAGGGA AAACAGCTCT GGCTACTCGG GAGCAGGTAC AGTGGTGGCT TTTGCAGTTA 
GCTCTGGAAC TTGAGGAGAG GCTAACCAAA GATCGAAATG ATAATGACCG CGTGGCCACT 
CAGCTGGTCG TCAGTATTCG TTTTCAGGGA GACAGGCGTC TCAGCAGCCT GCGGCGGTGC 
TGTGCCCTCC CTCGCTATGA CGCTCACAAG ATGAGCCAGG ACGCATTTGC TGCCATCAGG 
AACTGTAATA CATCTGGAAT CCAAACTGAG TGGTCCCCTC CTCTCACAAT GCTCTTCCTC 
TGCGCTACCA AGTTCTCTGC GGCTGCCCCT CCAGCTTGCA CGGATATCAC TGCGTTCCTG 
AGTAGCGACT CAAGTTGTCA GCCAAAGGTG CCAATTGCCA GCTCTGAGAC TAGGACCCAG 
GGGTCTGGCC CAGCTGTACC AACCTCTAAA GAGGCAGCCA CATCTCTAGC GTCTTTTTTT 
CAAAAAGCTG CCAAAAAGCA GAGAATGAAA GAGACTTCCT TTGTACCTCT TAACACAGCC 
ACAGAGAAGT TATCATCCAA GCCTTCACTT GTGTTCCAAA GCAGCCAAAC CACAGGAAGT 
CAGTCTTTCT TTAAGCAGAA GAGCCTGCTG CTCCAGCACA CACAGCTTAG TAACTCTGCG 
GCTCCTGACC CTCCACAGGC CAGCCCTGCA GCGCAACCAA GCTGTCTGCC AGCAGAGTGT 
GTAGACAGTG GTCCTGATGA TGGAGCGGTG AAGCCAGTAT CCTCTAAAGC AGTGTCTACA 
GAAATGAATG TGGCTGGGGA CAGTCCAAAC GTGCTCGATT CTCCAGCTTA CAACTCTCAG 
GAGGTGACCC AAAGGGCCAC TGAGGACCAA GTGCTCTGTG AGAAGTGTGA CTCCCTGGTT 
CCAGTGTGGG ACATGCCAGA ACACACGGAC TACCATTTTG CATTGGAGTT GCAGAAGTCT 
TTTTTGCAGC CTTGTACTTC AAAACCCCAG GCTATTCCTG CCGTGTCTCC TCAGGGCAAA 
AGAAATCCCA AGAGTCCCTC GGCTTCCAGT AGTAAGCGCC TTAGGCCCCA TGGCATGCAG 
ACGCTGGAAT CGTTTTTTAA GCCACTGACA CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polh ( NM_030715.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109640.1 Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), mRNA.

Price & Availability↑

CloneID	OMu08412
Clone ID Related Accession (Same CDS sequence)	NM_030715.3 , NM_030715.4
Accession Version	NM_030715.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Mus musculus(house mouse)
Product	DNA polymerase eta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK036296.1. On Apr 6, 2007 this sequence version replaced NM_030715.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036296.1, BC049159.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCCTG GGCAGAATCG AGTGGTTGCT CTTGTAGACA TGGACTGCTT TTTTGTACAA 
GTGGAACAGC GGCAAAATCC TCATTTGAGG AATAAACCTT GTGCAGTTGT ACAGTACAAA 
TCATGGAAAG GTGGTGGAAT TATTGCAGTT AGCTATGAAG CCCGAGCATT TGGTGTCACT 
AGAAACATGT GGGCGGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTCCT GGCACAAGTT 
CGTGAGTCCC GTGGAAAAGC TAACCTCACC AAGTACCGGG AAGCTAGTGT TGAAGTGATG 
GAGATAATGT CTTATTTTGC TGTGATTGAA CGTGCCAGCA TCGATGAGGC TTATATAGAT 
TTGACCAGTG CTGTACAAGA AAGACTTCAA AAGTTACAAG GTCAGCCTAT CTCAGCAGAT 
TTGCTGCCAA GCACTTACAT TGAAGGGTTG CCCCGAGGCC CTACAGTAGA AGAGACTGTT 
CAGAAAGAGG CTATTCGGAA ACAAGGCTTG CTTCAGTGGC TTGATTCTCT TCAGAGTGAT 
GATCCCACCT CTCCAGACCT CAGGCTGACT GTGGGAGCCA TGATTGTGGA AGAAATGAGA 
GCAGCCATAG AGAGTAAGAC TGGCTTCCAG TGTTCAGCTG GAATCTCACA CAATAAGGTC 
CTGGCAAAAC TTGCTTGTGG ACTAAATAAG CCCAACCGCC AAACCCTGGT CTCACATGGG 
TCAGTGCCAC AGCTCTTCAG CCAGATGCCA ATCCGTAAAA TCCGAAGTCT TGGAGGAAAG 
CTAGGGGCTT CTGTCATTGA AGTCCTTGGG ATAGAATACA TGGGTGACCT GACCCAGTTC 
ACTGAATCCC AGCTCCAGAG TCATTTTGGA GAGAAGAATG GGTCTTGGCT GTATGCCATG 
TGTCGAGGTA TTGAACACGA TCCAGTTAAA CCCAGGCAGT TACCTAAAAC TATTGGCTGC 
AGCAAGAACT TCCCAGGGAA AACAGCTCTG GCTACTCGGG AGCAGGTACA GTGGTGGCTT 
TTGCAGTTAG CTCTGGAACT TGAGGAGAGG CTAACCAAAG ATCGAAATGA TAATGACCGC 
GTGGCCACTC AGCTGGTCGT CAGTATTCGT TTTCAGGGAG ACAGGCGTCT CAGCAGCCTG 
CGGCGGTGCT GTGCCCTCCC TCGCTATGAC GCTCACAAGA TGAGCCAGGA CGCATTTGCT 
GCCATCAGGA ACTGTAATAC ATCTGGAATC CAAACTGAGT GGTCCCCTCC TCTCACAATG 
CTCTTCCTCT GCGCTACCAA GTTCTCTGCG GCTGCCCCTC CAGCTTGCAC GGATATCACT 
GCGTTCCTGA GTAGCGACTC AAGTTGTCAG CCAAAGGTGC CAATTGCCAG CTCTGAGACT 
AGGACCCAGG GGTCTGGCCC AGCTGTACCA ACCTCTAAAG AGGCAGCCAC ATCTCTAGCG 
TCTTTTTTTC AAAAAGCTGC CAAAAAGCAG AGAATGAAAG AGACTTCCTT TGTACCTCTT 
AACACAGCCA CAGAGAAGTT ATCATCCAAG CCTTCACTTG TGTTCCAAAG CAGCCAAACC 
ACAGGAAGTC AGTCTTTCTT TAAGCAGAAG AGCCTGCTGC TCCAGCACAC ACAGCTTAGT 
AACTCTGCGG CTCCTGACCC TCCACAGGCC AGCCCTGCAG CGCAACCAAG CTGTCTGCCA 
GCAGAGTGTG TAGACAGTGG TCCTGATGAT GGAGCGGTGA AGCCAGTATC CTCTAAAGCA 
GTGTCTACAG AAATGAATGT GGCTGGGGAC AGTCCAAACG TGCTCGATTC TCCAGCTTAC 
AACTCTCAGG AGGTGACCCA AAGGGCCACT GAGGACCAAG TGCTCTGTGA GAAGTGTGAC 
TCCCTGGTTC CAGTGTGGGA CATGCCAGAA CACACGGACT ACCATTTTGC ATTGGAGTTG 
CAGAAGTCTT TTTTGCAGCC TTGTACTTCA AAACCCCAGG CTATTCCTGC CGTGTCTCCT 
CAGGGCAAAA GAAATCCCAA GAGTCCCTCG GCTTCCAGTA GTAAGCGCCT TAGGCCCCAT 
GGCATGCAGA CGCTGGAATC GTTTTTTAAG CCACTGACAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109640.1
CDS	236..2320
Translation	MAPGQNRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSYFAVIERASIDEAYIDLTSAVQERLQKLQGQPISADLLPSTYIEGLPRGPTVEETVQKEAIRKQGLLQWLDSLQSDDPTSPDLRLTVGAMIVEEMRAAIESKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEVLGIEYMGDLTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLALELEERLTKDRNDNDRVATQLVVSIRFQGDRRLSSLRRCCALPRYDAHKMSQDAFAAIRNCNTSGIQTEWSPPLTMLFLCATKFSAAAPPACTDITAFLSSDSSCQPKVPIASSETRTQGSGPAVPTSKEAATSLASFFQKAAKKQRMKETSFVPLNTATEKLSSKPSLVFQSSQTTGSQSFFKQKSLLLQHTQLSNSAAPDPPQASPAAQPSCLPAECVDSGPDDGAVKPVSSKAVSTEMNVAGDSPNVLDSPAYNSQEVTQRATEDQVLCEKCDSLVPVWDMPEHTDYHFALELQKSFLQPCTSKPQAIPAVSPQGKRNPKSPSASSSKRLRPHGMQTLESFFKPLTH

Target ORF information:

RefSeq Version	NM_030715.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030715.3

ORF Insert Sequence:

ATGGCTCCTG GGCAGAATCG AGTGGTTGCT CTTGTAGACA TGGACTGCTT TTTTGTACAA 
GTGGAACAGC GGCAAAATCC TCATTTGAGG AATAAACCTT GTGCAGTTGT ACAGTACAAA 
TCATGGAAAG GTGGTGGAAT TATTGCAGTT AGCTATGAAG CCCGAGCATT TGGTGTCACT 
AGAAACATGT GGGCGGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTCCT GGCACAAGTT 
CGTGAGTCCC GTGGAAAAGC TAACCTCACC AAGTACCGGG AAGCTAGTGT TGAAGTGATG 
GAGATAATGT CTTATTTTGC TGTGATTGAA CGTGCCAGCA TCGATGAGGC TTATATAGAT 
TTGACCAGTG CTGTACAAGA AAGACTTCAA AAGTTACAAG GTCAGCCTAT CTCAGCAGAT 
TTGCTGCCAA GCACTTACAT TGAAGGGTTG CCCCGAGGCC CTACAGTAGA AGAGACTGTT 
CAGAAAGAGG CTATTCGGAA ACAAGGCTTG CTTCAGTGGC TTGATTCTCT TCAGAGTGAT 
GATCCCACCT CTCCAGACCT CAGGCTGACT GTGGGAGCCA TGATTGTGGA AGAAATGAGA 
GCAGCCATAG AGAGTAAGAC TGGCTTCCAG TGTTCAGCTG GAATCTCACA CAATAAGGTC 
CTGGCAAAAC TTGCTTGTGG ACTAAATAAG CCCAACCGCC AAACCCTGGT CTCACATGGG 
TCAGTGCCAC AGCTCTTCAG CCAGATGCCA ATCCGTAAAA TCCGAAGTCT TGGAGGAAAG 
CTAGGGGCTT CTGTCATTGA AGTCCTTGGG ATAGAATACA TGGGTGACCT GACCCAGTTC 
ACTGAATCCC AGCTCCAGAG TCATTTTGGA GAGAAGAATG GGTCTTGGCT GTATGCCATG 
TGTCGAGGTA TTGAACACGA TCCAGTTAAA CCCAGGCAGT TACCTAAAAC TATTGGCTGC 
AGCAAGAACT TCCCAGGGAA AACAGCTCTG GCTACTCGGG AGCAGGTACA GTGGTGGCTT 
TTGCAGTTAG CTCTGGAACT TGAGGAGAGG CTAACCAAAG ATCGAAATGA TAATGACCGC 
GTGGCCACTC AGCTGGTCGT CAGTATTCGT TTTCAGGGAG ACAGGCGTCT CAGCAGCCTG 
CGGCGGTGCT GTGCCCTCCC TCGCTATGAC GCTCACAAGA TGAGCCAGGA CGCATTTGCT 
GCCATCAGGA ACTGTAATAC ATCTGGAATC CAAACTGAGT GGTCCCCTCC TCTCACAATG 
CTCTTCCTCT GCGCTACCAA GTTCTCTGCG GCTGCCCCTC CAGCTTGCAC GGATATCACT 
GCGTTCCTGA GTAGCGACTC AAGTTGTCAG CCAAAGGTGC CAATTGCCAG CTCTGAGACT 
AGGACCCAGG GGTCTGGCCC AGCTGTACCA ACCTCTAAAG AGGCAGCCAC ATCTCTAGCG 
TCTTTTTTTC AAAAAGCTGC CAAAAAGCAG AGAATGAAAG AGACTTCCTT TGTACCTCTT 
AACACAGCCA CAGAGAAGTT ATCATCCAAG CCTTCACTTG TGTTCCAAAG CAGCCAAACC 
ACAGGAAGTC AGTCTTTCTT TAAGCAGAAG AGCCTGCTGC TCCAGCACAC ACAGCTTAGT 
AACTCTGCGG CTCCTGACCC TCCACAGGCC AGCCCTGCAG CGCAACCAAG CTGTCTGCCA 
GCAGAGTGTG TAGACAGTGG TCCTGATGAT GGAGCGGTGA AGCCAGTATC CTCTAAAGCA 
GTGTCTACAG AAATGAATGT GGCTGGGGAC AGTCCAAACG TGCTCGATTC TCCAGCTTAC 
AACTCTCAGG AGGTGACCCA AAGGGCCACT GAGGACCAAG TGCTCTGTGA GAAGTGTGAC 
TCCCTGGTTC CAGTGTGGGA CATGCCAGAA CACACGGACT ACCATTTTGC ATTGGAGTTG 
CAGAAGTCTT TTTTGCAGCC TTGTACTTCA AAACCCCAGG CTATTCCTGC CGTGTCTCCT 
CAGGGCAAAA GAAATCCCAA GAGTCCCTCG GCTTCCAGTA GTAAGCGCCT TAGGCCCCAT 
GGCATGCAGA CGCTGGAATC GTTTTTTAAG CCACTGACAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polh ( NM_030715.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109640.1 Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant 1, mRNA.

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CloneID	OMu08412
Clone ID Related Accession (Same CDS sequence)	NM_030715.3 , NM_030715.4
Accession Version	NM_030715.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	DNA polymerase eta isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ048830.1, AK036296.1 and AC116739.13. On Jul 17, 2018 this sequence version replaced NM_030715.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049159.1, AK036296.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTG GGCAGAATCG AGTGGTTGCT CTTGTAGACA TGGACTGCTT TTTTGTACAA 
GTGGAACAGC GGCAAAATCC TCATTTGAGG AATAAACCTT GTGCAGTTGT ACAGTACAAA 
TCATGGAAAG GTGGTGGAAT TATTGCAGTT AGCTATGAAG CCCGAGCATT TGGTGTCACT 
AGAAACATGT GGGCGGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTCCT GGCACAAGTT 
CGTGAGTCCC GTGGAAAAGC TAACCTCACC AAGTACCGGG AAGCTAGTGT TGAAGTGATG 
GAGATAATGT CTTATTTTGC TGTGATTGAA CGTGCCAGCA TCGATGAGGC TTATATAGAT 
TTGACCAGTG CTGTACAAGA AAGACTTCAA AAGTTACAAG GTCAGCCTAT CTCAGCAGAT 
TTGCTGCCAA GCACTTACAT TGAAGGGTTG CCCCGAGGCC CTACAGTAGA AGAGACTGTT 
CAGAAAGAGG CTATTCGGAA ACAAGGCTTG CTTCAGTGGC TTGATTCTCT TCAGAGTGAT 
GATCCCACCT CTCCAGACCT CAGGCTGACT GTGGGAGCCA TGATTGTGGA AGAAATGAGA 
GCAGCCATAG AGAGTAAGAC TGGCTTCCAG TGTTCAGCTG GAATCTCACA CAATAAGGTC 
CTGGCAAAAC TTGCTTGTGG ACTAAATAAG CCCAACCGCC AAACCCTGGT CTCACATGGG 
TCAGTGCCAC AGCTCTTCAG CCAGATGCCA ATCCGTAAAA TCCGAAGTCT TGGAGGAAAG 
CTAGGGGCTT CTGTCATTGA AGTCCTTGGG ATAGAATACA TGGGTGACCT GACCCAGTTC 
ACTGAATCCC AGCTCCAGAG TCATTTTGGA GAGAAGAATG GGTCTTGGCT GTATGCCATG 
TGTCGAGGTA TTGAACACGA TCCAGTTAAA CCCAGGCAGT TACCTAAAAC TATTGGCTGC 
AGCAAGAACT TCCCAGGGAA AACAGCTCTG GCTACTCGGG AGCAGGTACA GTGGTGGCTT 
TTGCAGTTAG CTCTGGAACT TGAGGAGAGG CTAACCAAAG ATCGAAATGA TAATGACCGC 
GTGGCCACTC AGCTGGTCGT CAGTATTCGT TTTCAGGGAG ACAGGCGTCT CAGCAGCCTG 
CGGCGGTGCT GTGCCCTCCC TCGCTATGAC GCTCACAAGA TGAGCCAGGA CGCATTTGCT 
GCCATCAGGA ACTGTAATAC ATCTGGAATC CAAACTGAGT GGTCCCCTCC TCTCACAATG 
CTCTTCCTCT GCGCTACCAA GTTCTCTGCG GCTGCCCCTC CAGCTTGCAC GGATATCACT 
GCGTTCCTGA GTAGCGACTC AAGTTGTCAG CCAAAGGTGC CAATTGCCAG CTCTGAGACT 
AGGACCCAGG GGTCTGGCCC AGCTGTACCA ACCTCTAAAG AGGCAGCCAC ATCTCTAGCG 
TCTTTTTTTC AAAAAGCTGC CAAAAAGCAG AGAATGAAAG AGACTTCCTT TGTACCTCTT 
AACACAGCCA CAGAGAAGTT ATCATCCAAG CCTTCACTTG TGTTCCAAAG CAGCCAAACC 
ACAGGAAGTC AGTCTTTCTT TAAGCAGAAG AGCCTGCTGC TCCAGCACAC ACAGCTTAGT 
AACTCTGCGG CTCCTGACCC TCCACAGGCC AGCCCTGCAG CGCAACCAAG CTGTCTGCCA 
GCAGAGTGTG TAGACAGTGG TCCTGATGAT GGAGCGGTGA AGCCAGTATC CTCTAAAGCA 
GTGTCTACAG AAATGAATGT GGCTGGGGAC AGTCCAAACG TGCTCGATTC TCCAGCTTAC 
AACTCTCAGG AGGTGACCCA AAGGGCCACT GAGGACCAAG TGCTCTGTGA GAAGTGTGAC 
TCCCTGGTTC CAGTGTGGGA CATGCCAGAA CACACGGACT ACCATTTTGC ATTGGAGTTG 
CAGAAGTCTT TTTTGCAGCC TTGTACTTCA AAACCCCAGG CTATTCCTGC CGTGTCTCCT 
CAGGGCAAAA GAAATCCCAA GAGTCCCTCG GCTTCCAGTA GTAAGCGCCT TAGGCCCCAT 
GGCATGCAGA CGCTGGAATC GTTTTTTAAG CCACTGACAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109640.1
CDS	252..2336
Translation	MAPGQNRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSYFAVIERASIDEAYIDLTSAVQERLQKLQGQPISADLLPSTYIEGLPRGPTVEETVQKEAIRKQGLLQWLDSLQSDDPTSPDLRLTVGAMIVEEMRAAIESKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEVLGIEYMGDLTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLALELEERLTKDRNDNDRVATQLVVSIRFQGDRRLSSLRRCCALPRYDAHKMSQDAFAAIRNCNTSGIQTEWSPPLTMLFLCATKFSAAAPPACTDITAFLSSDSSCQPKVPIASSETRTQGSGPAVPTSKEAATSLASFFQKAAKKQRMKETSFVPLNTATEKLSSKPSLVFQSSQTTGSQSFFKQKSLLLQHTQLSNSAAPDPPQASPAAQPSCLPAECVDSGPDDGAVKPVSSKAVSTEMNVAGDSPNVLDSPAYNSQEVTQRATEDQVLCEKCDSLVPVWDMPEHTDYHFALELQKSFLQPCTSKPQAIPAVSPQGKRNPKSPSASSSKRLRPHGMQTLESFFKPLTH

Target ORF information:

RefSeq Version	NM_030715.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), eta (RAD 30 related) (Polh), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030715.4

ORF Insert Sequence:

ATGGCTCCTG GGCAGAATCG AGTGGTTGCT CTTGTAGACA TGGACTGCTT TTTTGTACAA 
GTGGAACAGC GGCAAAATCC TCATTTGAGG AATAAACCTT GTGCAGTTGT ACAGTACAAA 
TCATGGAAAG GTGGTGGAAT TATTGCAGTT AGCTATGAAG CCCGAGCATT TGGTGTCACT 
AGAAACATGT GGGCGGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTCCT GGCACAAGTT 
CGTGAGTCCC GTGGAAAAGC TAACCTCACC AAGTACCGGG AAGCTAGTGT TGAAGTGATG 
GAGATAATGT CTTATTTTGC TGTGATTGAA CGTGCCAGCA TCGATGAGGC TTATATAGAT 
TTGACCAGTG CTGTACAAGA AAGACTTCAA AAGTTACAAG GTCAGCCTAT CTCAGCAGAT 
TTGCTGCCAA GCACTTACAT TGAAGGGTTG CCCCGAGGCC CTACAGTAGA AGAGACTGTT 
CAGAAAGAGG CTATTCGGAA ACAAGGCTTG CTTCAGTGGC TTGATTCTCT TCAGAGTGAT 
GATCCCACCT CTCCAGACCT CAGGCTGACT GTGGGAGCCA TGATTGTGGA AGAAATGAGA 
GCAGCCATAG AGAGTAAGAC TGGCTTCCAG TGTTCAGCTG GAATCTCACA CAATAAGGTC 
CTGGCAAAAC TTGCTTGTGG ACTAAATAAG CCCAACCGCC AAACCCTGGT CTCACATGGG 
TCAGTGCCAC AGCTCTTCAG CCAGATGCCA ATCCGTAAAA TCCGAAGTCT TGGAGGAAAG 
CTAGGGGCTT CTGTCATTGA AGTCCTTGGG ATAGAATACA TGGGTGACCT GACCCAGTTC 
ACTGAATCCC AGCTCCAGAG TCATTTTGGA GAGAAGAATG GGTCTTGGCT GTATGCCATG 
TGTCGAGGTA TTGAACACGA TCCAGTTAAA CCCAGGCAGT TACCTAAAAC TATTGGCTGC 
AGCAAGAACT TCCCAGGGAA AACAGCTCTG GCTACTCGGG AGCAGGTACA GTGGTGGCTT 
TTGCAGTTAG CTCTGGAACT TGAGGAGAGG CTAACCAAAG ATCGAAATGA TAATGACCGC 
GTGGCCACTC AGCTGGTCGT CAGTATTCGT TTTCAGGGAG ACAGGCGTCT CAGCAGCCTG 
CGGCGGTGCT GTGCCCTCCC TCGCTATGAC GCTCACAAGA TGAGCCAGGA CGCATTTGCT 
GCCATCAGGA ACTGTAATAC ATCTGGAATC CAAACTGAGT GGTCCCCTCC TCTCACAATG 
CTCTTCCTCT GCGCTACCAA GTTCTCTGCG GCTGCCCCTC CAGCTTGCAC GGATATCACT 
GCGTTCCTGA GTAGCGACTC AAGTTGTCAG CCAAAGGTGC CAATTGCCAG CTCTGAGACT 
AGGACCCAGG GGTCTGGCCC AGCTGTACCA ACCTCTAAAG AGGCAGCCAC ATCTCTAGCG 
TCTTTTTTTC AAAAAGCTGC CAAAAAGCAG AGAATGAAAG AGACTTCCTT TGTACCTCTT 
AACACAGCCA CAGAGAAGTT ATCATCCAAG CCTTCACTTG TGTTCCAAAG CAGCCAAACC 
ACAGGAAGTC AGTCTTTCTT TAAGCAGAAG AGCCTGCTGC TCCAGCACAC ACAGCTTAGT 
AACTCTGCGG CTCCTGACCC TCCACAGGCC AGCCCTGCAG CGCAACCAAG CTGTCTGCCA 
GCAGAGTGTG TAGACAGTGG TCCTGATGAT GGAGCGGTGA AGCCAGTATC CTCTAAAGCA 
GTGTCTACAG AAATGAATGT GGCTGGGGAC AGTCCAAACG TGCTCGATTC TCCAGCTTAC 
AACTCTCAGG AGGTGACCCA AAGGGCCACT GAGGACCAAG TGCTCTGTGA GAAGTGTGAC 
TCCCTGGTTC CAGTGTGGGA CATGCCAGAA CACACGGACT ACCATTTTGC ATTGGAGTTG 
CAGAAGTCTT TTTTGCAGCC TTGTACTTCA AAACCCCAGG CTATTCCTGC CGTGTCTCCT 
CAGGGCAAAA GAAATCCCAA GAGTCCCTCG GCTTCCAGTA GTAAGCGCCT TAGGCCCCAT 
GGCATGCAGA CGCTGGAATC GTTTTTTAAG CCACTGACAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)
Zhou L,Dai H,Wu J,Zhou M,Yuan H,Du J,Yang L,Wu X,Xu H,Hua Y,Xu J,Zheng L,Shen B

A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro.
DNA repair4637-46(2016 10)
Fritzen R,Delbos F,De Smet A,Palancade B,Canman CE,Aoufouchi S,Weill JC,Reynaud CA,Storck S

The Polymerase Activity of Mammalian DNA Pol ? Is Specifically Required for Cell and Embryonic Viability.
PLoS genetics12(1)e1005759(2016 Jan)
Lange SS,Tomida J,Boulware KS,Bhetawal S,Wood RD

Ablation of XP-V gene causes adipose tissue senescence and metabolic abnormalities.
Proceedings of the National Academy of Sciences of the United States of America112(33)E4556-64(2015 Aug)
Chen YW,Harris RA,Hatahet Z,Chou KM

UV-induced mutations in epidermal cells of mice defective in DNA polymerase ? and/or ?.
DNA repair29139-46(2015 May)
Kanao R,Yokoi M,Ohkumo T,Sakurai Y,Dotsu K,Kura S,Nakatsu Y,Tsuzuki T,Masutani C,Hanaoka F

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Polh cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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