POLH cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLH
Entrez Gene ID	513606
Full Name	DNA polymerase eta
General protein information	Preferred Names DNA polymerase eta Names DNA polymerase eta DNA-directed DNA polymerase eta polymerase (DNA directed), eta
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024983568.1	XP_024839336.1	DNA polymerase eta isoform X1
NM_001034450.1	NP_001029622.1	DNA polymerase eta

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLH	NP_006493.1
	POLH	XP_002803805.1
Canis lupus familiaris (dog)	POLH	XP_532150.2
Rattus norvegicus (Norway rat)	Polh	NP_001101674.1
Danio rerio (zebrafish)	polh	NP_001035337.1
Xenopus tropicalis (tropical clawed frog)	polh	XP_002942198.2
Mus musculus (house mouse)	Polh	NP_109640.1
Bos taurus (cattle)	POLH	NP_001029622.1
Gallus gallus (chicken)	POLH	NP_001001304.1
Pan troglodytes (chimpanzee)	POLH	XP_518497.1
Caenorhabditis elegans (roundworm)	polh-1	NP_497480.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa02198	NM_001034450.1 Latest version!	Bos taurus DNA polymerase eta (POLH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa229474	XM_024983568.1 Latest version!	Bos taurus DNA polymerase eta (POLH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLH ( NM_001034450.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029622.1 Bos taurus DNA polymerase eta (POLH), mRNA.

Price & Availability↑

CloneID	OBa02198
Clone ID Related Accession (Same CDS sequence)	NM_001034450.1
Accession Version	NM_001034450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	DNA polymerase eta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103396.1. On Oct 17, 2005 this sequence version replaced XM_591312.2. ##Evidence-Data-START## CDS exon combination :: BC103396.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGACTGATG GAAGAGACCG GGTAGTTGCT CTAGTGGACA TGGACTGTTT TTTCGTTCAA 
GTGGAACAGC GGCAAAATCC TCATTTGAGG AATAAACCCT GTGCAGTTGT ACAGTACACA 
TCATGGAAGG GCGGTGGAAT AGTTGCAGTA AGTTATGAAG CTCGTGCATT TGGGGTCACT 
AGAAACATGT GGGCAGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTACT GGCACAAATT 
CGTGAGCTCC ATGGAAAAGC TAACCTCACC AAGTACCGGG ATGCCAGTAT GGAAGTGATG 
GAGGTAATGT CTCGTTTTGC TGTAATGGAA CGTGGCAGCA TTGATGAGGC TTATCTAGAC 
CTGACCAGTG CTGTGCAAGA GAGACTACAA AAGCTCCAAA ATCAGCCTAT CTCAGCAGAC 
TTGTTGCCAA CCACCTGCAT TGAAGGGTTT CCCCAAGGGC CTACAACAAC AGAAGGGACT 
GTTGAGAAAG AGGAAAGGCG AAAACAAGGC TTATTGCAAT GGCTTGATTC TCTTCAGACT 
GAAAGCACCA GCTCTCCAGA CCTACAGCTC ACTATGGGAG CAGTGATTGT GGAAGAAATG 
CGAGTAGCCA TCGAGAAGCA GACTGGTTTT CAGTGTTCTG CTGGGATTTC ACACAATAAG 
GTTCTGGCAA AACTGGCCTG TGGATTAAAC AAGCCCAACC GGCAGACCCT GGTCTCACAC 
GGGTCAGTCC CACAACTCTT CAACCAAGTG CCTATTAGCA AAATCCGTAA TCTTGGAGGA 
AAACTAGGGG CCTCTGTCAT TGAAATCCTG GGGGTAGAAT ACATGGGAGA GCTGACCCAG 
TTCTCTGAAT CCCAGCTCCA GAGTCATTTT GGGGAGAGGA ATGGATCTTG GCTATATGCC 
ATGTGCCGAG GGATTGAACA TGATCCAGTT AAACCCAGGC CAATAACCAA AACCCTTGGC 
TGCGGCAAGA ACTTCCCAGG AAAAACAGCT CTGTCTACTC GGGATCAGGT GCAATGGTGG 
CTTTTGCAAT TAGCCCAGGA ACTAGAGGAG AGACTCATCA AGGACCGAAA TGCTAATGAC 
AGGGTGGCCA CTCAGCTGGT AGTGAGCATG CGCATACAAG GAGACAGGCG TCTGAGCAGC 
TTGCGACGCT GTTGTGCCCT CACCCACTAT GATGCTCACA AGATGAGCCA TGATGCATTT 
GCTACCATCA GGAACTGTAA CACTTCAGGA ACCCAGACTG AATGGGCCCC TCCCCTTATA 
ATGCTTTTCC TCTGTGCTAC CAAATTCTGT TCCTCTACCC CTTCACCTGG CCCAGACATC 
ACCACCTTCT TGAGCAGTGA CCCAAGTTCT CTGCCAAAAG TGCCAATCAC CAGTTCAGAA 
GCCAAGACCC AGGGAAGTGG CCCAGCAGTG ACAACCCCTA AGAAAGCAAC GACTTCTCTG 
GAATCGTTCT TCCAGAAAGC TGCAGAAAAG CAGAAAGTCA AAGAAGCTTC ACTTTCTTCT 
CCTACTGCCA CTACACAGGC CGCCATGAGC CCATCATCCA AGCCCTCATT ACCTTTCCAA 
AGCAGTCATA CTATGGGAAC CGAGCCCTTC TTTAAGCAGA AAAGTCTGCT TCTAAAGCAG 
AAGCAGCTCA CTAATCCTTC AGTTTTCTTC CCTTCACAGA AGCCACAGTC CAGTCCTAAA 
GAGTTACCAA ACTGTTTTCC AACAGAGAAT CCAGATTGTG CCCCTGACTG TGAAAAGATG 
TTGAAGCTAG GATCCCCCAA AACAACTCCC ACAGAGATGG ATTTGGCCCA GAACAGCCTC 
AGCAGCCTTG CTTCTTTAAC TTCCACATCT GCTCTGGAGG TGGCTCAGAA GGCAACTATG 
ACCTCCAGTT TTCTGGCTGC TGAGGACCAA GTGCCCTGCG AGAAGTGTGG CTCTCTGGTA 
CCTGTGTGGG ACATGCCAGA ACACTCAGAC TATCACTTTG CATTGGAGTT ACAGAACTCC 
TTTTTGCAGC CCCACTCCTC AAACCCGCAG GTTGTTCCTG CCAGTTCTCC TCAAAGCAAA 
AGAAATCCCA AGAGCCCTCT GGCCTCAAAT AATAAACGGC CCAGGCCTGA GGGCATGCAG 
ACACTGGAAT CATTTTTTAA GCCATTAACA CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029622.1
CDS	211..2346
Translation	MTDGRDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYTSWKGGGIVAVSYEARAFGVTRNMWADDAKKLCPDLLLAQIRELHGKANLTKYRDASMEVMEVMSRFAVMERGSIDEAYLDLTSAVQERLQKLQNQPISADLLPTTCIEGFPQGPTTTEGTVEKEERRKQGLLQWLDSLQTESTSSPDLQLTMGAVIVEEMRVAIEKQTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFNQVPISKIRNLGGKLGASVIEILGVEYMGELTQFSESQLQSHFGERNGSWLYAMCRGIEHDPVKPRPITKTLGCGKNFPGKTALSTRDQVQWWLLQLAQELEERLIKDRNANDRVATQLVVSMRIQGDRRLSSLRRCCALTHYDAHKMSHDAFATIRNCNTSGTQTEWAPPLIMLFLCATKFCSSTPSPGPDITTFLSSDPSSLPKVPITSSEAKTQGSGPAVTTPKKATTSLESFFQKAAEKQKVKEASLSSPTATTQAAMSPSSKPSLPFQSSHTMGTEPFFKQKSLLLKQKQLTNPSVFFPSQKPQSSPKELPNCFPTENPDCAPDCEKMLKLGSPKTTPTEMDLAQNSLSSLASLTSTSALEVAQKATMTSSFLAAEDQVPCEKCGSLVPVWDMPEHSDYHFALELQNSFLQPHSSNPQVVPASSPQSKRNPKSPLASNNKRPRPEGMQTLESFFKPLTH

Target ORF information:

RefSeq Version	NM_001034450.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase eta (POLH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034450.1

ORF Insert Sequence:

ATGACTGATG GAAGAGACCG GGTAGTTGCT CTAGTGGACA TGGACTGTTT TTTCGTTCAA 
GTGGAACAGC GGCAAAATCC TCATTTGAGG AATAAACCCT GTGCAGTTGT ACAGTACACA 
TCATGGAAGG GCGGTGGAAT AGTTGCAGTA AGTTATGAAG CTCGTGCATT TGGGGTCACT 
AGAAACATGT GGGCAGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTACT GGCACAAATT 
CGTGAGCTCC ATGGAAAAGC TAACCTCACC AAGTACCGGG ATGCCAGTAT GGAAGTGATG 
GAGGTAATGT CTCGTTTTGC TGTAATGGAA CGTGGCAGCA TTGATGAGGC TTATCTAGAC 
CTGACCAGTG CTGTGCAAGA GAGACTACAA AAGCTCCAAA ATCAGCCTAT CTCAGCAGAC 
TTGTTGCCAA CCACCTGCAT TGAAGGGTTT CCCCAAGGGC CTACAACAAC AGAAGGGACT 
GTTGAGAAAG AGGAAAGGCG AAAACAAGGC TTATTGCAAT GGCTTGATTC TCTTCAGACT 
GAAAGCACCA GCTCTCCAGA CCTACAGCTC ACTATGGGAG CAGTGATTGT GGAAGAAATG 
CGAGTAGCCA TCGAGAAGCA GACTGGTTTT CAGTGTTCTG CTGGGATTTC ACACAATAAG 
GTTCTGGCAA AACTGGCCTG TGGATTAAAC AAGCCCAACC GGCAGACCCT GGTCTCACAC 
GGGTCAGTCC CACAACTCTT CAACCAAGTG CCTATTAGCA AAATCCGTAA TCTTGGAGGA 
AAACTAGGGG CCTCTGTCAT TGAAATCCTG GGGGTAGAAT ACATGGGAGA GCTGACCCAG 
TTCTCTGAAT CCCAGCTCCA GAGTCATTTT GGGGAGAGGA ATGGATCTTG GCTATATGCC 
ATGTGCCGAG GGATTGAACA TGATCCAGTT AAACCCAGGC CAATAACCAA AACCCTTGGC 
TGCGGCAAGA ACTTCCCAGG AAAAACAGCT CTGTCTACTC GGGATCAGGT GCAATGGTGG 
CTTTTGCAAT TAGCCCAGGA ACTAGAGGAG AGACTCATCA AGGACCGAAA TGCTAATGAC 
AGGGTGGCCA CTCAGCTGGT AGTGAGCATG CGCATACAAG GAGACAGGCG TCTGAGCAGC 
TTGCGACGCT GTTGTGCCCT CACCCACTAT GATGCTCACA AGATGAGCCA TGATGCATTT 
GCTACCATCA GGAACTGTAA CACTTCAGGA ACCCAGACTG AATGGGCCCC TCCCCTTATA 
ATGCTTTTCC TCTGTGCTAC CAAATTCTGT TCCTCTACCC CTTCACCTGG CCCAGACATC 
ACCACCTTCT TGAGCAGTGA CCCAAGTTCT CTGCCAAAAG TGCCAATCAC CAGTTCAGAA 
GCCAAGACCC AGGGAAGTGG CCCAGCAGTG ACAACCCCTA AGAAAGCAAC GACTTCTCTG 
GAATCGTTCT TCCAGAAAGC TGCAGAAAAG CAGAAAGTCA AAGAAGCTTC ACTTTCTTCT 
CCTACTGCCA CTACACAGGC CGCCATGAGC CCATCATCCA AGCCCTCATT ACCTTTCCAA 
AGCAGTCATA CTATGGGAAC CGAGCCCTTC TTTAAGCAGA AAAGTCTGCT TCTAAAGCAG 
AAGCAGCTCA CTAATCCTTC AGTTTTCTTC CCTTCACAGA AGCCACAGTC CAGTCCTAAA 
GAGTTACCAA ACTGTTTTCC AACAGAGAAT CCAGATTGTG CCCCTGACTG TGAAAAGATG 
TTGAAGCTAG GATCCCCCAA AACAACTCCC ACAGAGATGG ATTTGGCCCA GAACAGCCTC 
AGCAGCCTTG CTTCTTTAAC TTCCACATCT GCTCTGGAGG TGGCTCAGAA GGCAACTATG 
ACCTCCAGTT TTCTGGCTGC TGAGGACCAA GTGCCCTGCG AGAAGTGTGG CTCTCTGGTA 
CCTGTGTGGG ACATGCCAGA ACACTCAGAC TATCACTTTG CATTGGAGTT ACAGAACTCC 
TTTTTGCAGC CCCACTCCTC AAACCCGCAG GTTGTTCCTG CCAGTTCTCC TCAAAGCAAA 
AGAAATCCCA AGAGCCCTCT GGCCTCAAAT AATAAACGGC CCAGGCCTGA GGGCATGCAG 
ACACTGGAAT CATTTTTTAA GCCATTAACA CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLH ( XM_024983568.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839336.1 Bos taurus DNA polymerase eta (POLH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229474
Clone ID Related Accession (Same CDS sequence)	XM_024983568.1
Accession Version	XM_024983568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA polymerase eta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG TGCATTTGGG GTCACTAGAA ACATGTGGGC AGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTACTGGCA CAAATTCGTG AGCTCCATGG AAAAGCTAAC CTCACCAAAG 
GAAAGGCGAA AACAAGGCTT ATTGCAATGG CTTGATTCTC TTCAGACTGA AAGCACCAGC 
TCTCCAGACC TACAGCTCAC TATGGGAGCA GTGATTGTGG AAGAAATGCG AGCAGCCATC 
GAGAGGCAGA CCGGTTTTCA GTGTTCTGCT GGGATTTCAC ACAATAAGGT TCTGGCAAAA 
CTGGCCTGTG GATTAAACAA GCCCAACCGG CAGACCCTGG TCTCACACGG GTCAGTCCCG 
CAACTCTTCA ACCAAGTGCC TATTAGCAAA ATCCGTAATC TTGGAGGAAA ACTAGGGGCC 
TCTGTCATTG AAATCCTGGG GGTAGAATAC ATGGGAGAGC TGACCCAGTT CTCTGAACCC 
CAGCTCCAGA GTCATTTTGG GGAGAGGAAT GGATCTTGGC TATATGCCAT GTGCCGGGGG 
ATTGAACATG ATCCAGTTAA ACCCAGGCCA ATAACCAAAA CCCTTGGCTG CAGCAAGAAC 
TTCCCAGGAA AAACAGCTCT GTCTACTCGG GATCAGGTGC AATGGTGGCT TTTGCAATTA 
GCCCAGGAAC TAGAGGAGAG ACTCATCAAG GACCGAAATG CTAACGACAG GGTGGCCACT 
CAGCTGGTAG TGAGCATGCG CATACAAGGA GACAGGCGTC TGAGCAGCTT GCGACGCTGT 
TGTGCCCTCA CCCACTATGA TGCTCACAAG ATGAGCCATG ATGCATTTGC TACCATCAGG 
AACTGTAACA CTTCAGGAAC CCAGACTGAA TGGGCCCCTC CCCTTATAAT GCTTTTCCTC 
TGTGCTACCA AATTCTGTTC CTCTACCCCT TCACCTGGCC CAGACATCAC CACCTTCTTG 
AGCAGTGACC CAAGTTCTCT GCCGAAAGTG CCAATCACCA GTTCAGAAGC CAAGACCCAG 
GGAAGTGGCC CAGCAGTGAC AACCCCTAAG AAGGCAACGA CTTCTCTGGA ATCGTTCTTC 
CAGAAAGCTG CAGAAAAGCA CAAAGTCAAA GAAGCTTCAC TTTCTTCTCC TACTGCCACT 
ACACAGGCCC CCATGAGCCC ATCATCCAAG CCCTCATTAC CTTTCCAAAG CAGTCATACT 
ATGGGAACCG AGCCCTTCTT TAAGCAGAAA AGTCTGCTTC TAAAGCAGAA GCAGCTCACT 
AATCCTTCAG TTTTCTTCCC TTCACAGAAG CCACAGTCCA GTCCTAAAGA GTTACCAAAC 
TGTTTTCCAA CAGAGAATCC AGATTGTGCC CCTGACTGTG AAAAGATGTT GAAGCTAGGA 
TCCCCCAAAA CAACTCCCAC AGAGATGGAT TTGGCCCAGA ACAGCCTCAG CAGCCTTGCT 
TCTTTAACTT CCACATCTGC TCTGGAGGTG GCTCAGAAGG CAACTATGAC CTCCAGTTTT 
CTGGCTGCTG AGGACCAAGT GCCCTGCGAG AAGTGTGGCT CTCTGGTACC TGTGTGGGAC 
ATGCCAGAAC ACTCAGACTA TCACTTTGCA TTGGAGTTAC AGAACTCCTT TTTGCAGCCC 
CACTCCTCAA ACCCGCAGGT TGTTCCTGCC AGTTCTCCTC AAAGCAAAAG AAATCCCAAG 
AGCCCTCTGG CCTCAAATAA TAAACGGCCC AGGCCTGAGG GCATGCAGAC ACTGGAATCA 
TTTTTTAAGC CATTAACACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839336.1
CDS	158..1921
Translation	MKLVHLGSLETCGQMMLRSYVQIFYWHKFVSSMEKLTSPKERRKQGLLQWLDSLQTESTSSPDLQLTMGAVIVEEMRAAIERQTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFNQVPISKIRNLGGKLGASVIEILGVEYMGELTQFSEPQLQSHFGERNGSWLYAMCRGIEHDPVKPRPITKTLGCSKNFPGKTALSTRDQVQWWLLQLAQELEERLIKDRNANDRVATQLVVSMRIQGDRRLSSLRRCCALTHYDAHKMSHDAFATIRNCNTSGTQTEWAPPLIMLFLCATKFCSSTPSPGPDITTFLSSDPSSLPKVPITSSEAKTQGSGPAVTTPKKATTSLESFFQKAAEKHKVKEASLSSPTATTQAPMSPSSKPSLPFQSSHTMGTEPFFKQKSLLLKQKQLTNPSVFFPSQKPQSSPKELPNCFPTENPDCAPDCEKMLKLGSPKTTPTEMDLAQNSLSSLASLTSTSALEVAQKATMTSSFLAAEDQVPCEKCGSLVPVWDMPEHSDYHFALELQNSFLQPHSSNPQVVPASSPQSKRNPKSPLASNNKRPRPEGMQTLESFFKPLTH

Target ORF information:

RefSeq Version	XM_024983568.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase eta (POLH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983568.1

ORF Insert Sequence:

ATGAAGCTCG TGCATTTGGG GTCACTAGAA ACATGTGGGC AGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTACTGGCA CAAATTCGTG AGCTCCATGG AAAAGCTAAC CTCACCAAAG 
GAAAGGCGAA AACAAGGCTT ATTGCAATGG CTTGATTCTC TTCAGACTGA AAGCACCAGC 
TCTCCAGACC TACAGCTCAC TATGGGAGCA GTGATTGTGG AAGAAATGCG AGCAGCCATC 
GAGAGGCAGA CCGGTTTTCA GTGTTCTGCT GGGATTTCAC ACAATAAGGT TCTGGCAAAA 
CTGGCCTGTG GATTAAACAA GCCCAACCGG CAGACCCTGG TCTCACACGG GTCAGTCCCG 
CAACTCTTCA ACCAAGTGCC TATTAGCAAA ATCCGTAATC TTGGAGGAAA ACTAGGGGCC 
TCTGTCATTG AAATCCTGGG GGTAGAATAC ATGGGAGAGC TGACCCAGTT CTCTGAACCC 
CAGCTCCAGA GTCATTTTGG GGAGAGGAAT GGATCTTGGC TATATGCCAT GTGCCGGGGG 
ATTGAACATG ATCCAGTTAA ACCCAGGCCA ATAACCAAAA CCCTTGGCTG CAGCAAGAAC 
TTCCCAGGAA AAACAGCTCT GTCTACTCGG GATCAGGTGC AATGGTGGCT TTTGCAATTA 
GCCCAGGAAC TAGAGGAGAG ACTCATCAAG GACCGAAATG CTAACGACAG GGTGGCCACT 
CAGCTGGTAG TGAGCATGCG CATACAAGGA GACAGGCGTC TGAGCAGCTT GCGACGCTGT 
TGTGCCCTCA CCCACTATGA TGCTCACAAG ATGAGCCATG ATGCATTTGC TACCATCAGG 
AACTGTAACA CTTCAGGAAC CCAGACTGAA TGGGCCCCTC CCCTTATAAT GCTTTTCCTC 
TGTGCTACCA AATTCTGTTC CTCTACCCCT TCACCTGGCC CAGACATCAC CACCTTCTTG 
AGCAGTGACC CAAGTTCTCT GCCGAAAGTG CCAATCACCA GTTCAGAAGC CAAGACCCAG 
GGAAGTGGCC CAGCAGTGAC AACCCCTAAG AAGGCAACGA CTTCTCTGGA ATCGTTCTTC 
CAGAAAGCTG CAGAAAAGCA CAAAGTCAAA GAAGCTTCAC TTTCTTCTCC TACTGCCACT 
ACACAGGCCC CCATGAGCCC ATCATCCAAG CCCTCATTAC CTTTCCAAAG CAGTCATACT 
ATGGGAACCG AGCCCTTCTT TAAGCAGAAA AGTCTGCTTC TAAAGCAGAA GCAGCTCACT 
AATCCTTCAG TTTTCTTCCC TTCACAGAAG CCACAGTCCA GTCCTAAAGA GTTACCAAAC 
TGTTTTCCAA CAGAGAATCC AGATTGTGCC CCTGACTGTG AAAAGATGTT GAAGCTAGGA 
TCCCCCAAAA CAACTCCCAC AGAGATGGAT TTGGCCCAGA ACAGCCTCAG CAGCCTTGCT 
TCTTTAACTT CCACATCTGC TCTGGAGGTG GCTCAGAAGG CAACTATGAC CTCCAGTTTT 
CTGGCTGCTG AGGACCAAGT GCCCTGCGAG AAGTGTGGCT CTCTGGTACC TGTGTGGGAC 
ATGCCAGAAC ACTCAGACTA TCACTTTGCA TTGGAGTTAC AGAACTCCTT TTTGCAGCCC 
CACTCCTCAA ACCCGCAGGT TGTTCCTGCC AGTTCTCCTC AAAGCAAAAG AAATCCCAAG 
AGCCCTCTGG CCTCAAATAA TAAACGGCCC AGGCCTGAGG GCATGCAGAC ACTGGAATCA 
TTTTTTAAGC CATTAACACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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POLH cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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