POLH cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLH
Entrez Gene ID	395102
Full Name	DNA polymerase eta
General protein information	Preferred Names DNA polymerase eta Names DNA polymerase eta polymerase (DNA directed), eta polymerase (DNA) eta polymerase eta
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001304.2	NP_001001304.2	DNA polymerase eta

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLH	NP_006493.1
	POLH	XP_002803805.1
Canis lupus familiaris (dog)	POLH	XP_532150.2
Rattus norvegicus (Norway rat)	Polh	NP_001101674.1
Danio rerio (zebrafish)	polh	NP_001035337.1
Xenopus tropicalis (tropical clawed frog)	polh	XP_002942198.2
Mus musculus (house mouse)	Polh	NP_109640.1
Bos taurus (cattle)	POLH	NP_001029622.1
Gallus gallus (chicken)	POLH	NP_001001304.1
Pan troglodytes (chimpanzee)	POLH	XP_518497.1
Caenorhabditis elegans (roundworm)	polh-1	NP_497480.2

Gene Ontology
Bibliography

Related articles in PubMed

TopBP1-mediated DNA processing during mitosis.
Gallina I, Christiansen SK, Pedersen RT, Lisby M, Oestergaard VH
Cell cycle (Georgetown, Tex.)15(2)176-83(2016)

GCN5 protects vertebrate cells against UV-irradiation via controlling gene expression of DNA polymerase ?.
Kikuchi H, Kuribayashi F, Imajoh-Ohmi S, Nishitoh H, Takami Y, Nakayama T
The Journal of biological chemistry287(47)39842-9(2012 Nov)

Simultaneous disruption of two DNA polymerases, Pol? and Pol?, in Avian DT40 cells unmasks the role of Pol? in cellular response to various DNA lesions.
Hirota K, Sonoda E, Kawamoto T, Motegi A, Masutani C, Hanaoka F, Sz?ts D, Iwai S, Sale JE, Lehmann A, Takeda S
PLoS genetics6(10)(2010 Oct)

Dual roles for DNA polymerase eta in homologous DNA recombination and translesion DNA synthesis.
Kawamoto T, Araki K, Sonoda E, Yamashita YM, Harada K, Kikuchi K, Masutani C, Hanaoka F, Nozaki K, Hashimoto N, Takeda S
Molecular cell20(5)793-9(2005 Dec)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

To avoid genome instability, translesion DNA polymerase eta foci do not colocalize with TopBP1 or FANCD2 in mitosis.
Title: TopBP1-mediated DNA processing during mitosis.

GCN5 takes part in transcription regulation of POLH gene through alterations in the chromatin structure by direct interaction with its 5'-flanking region, and protects vertebrate cells against UV-induced DNA damage via controlling POLH gene expression.
Title: GCN5 protects vertebrate cells against UV-irradiation via controlling gene expression of DNA polymerase ?.

Data shew that the loss of Poleta significantly rescued all mutant phenotypes of POLzeta(-/-) cells and results in the restoration of the DNA damage tolerance by a backup pathway including HR.
Title: Simultaneous disruption of two DNA polymerases, Pol? and Pol?, in Avian DT40 cells unmasks the role of Pol? in cellular response to various DNA lesions.

The following POLH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28328	NM_001001304.2 Latest version!	Gallus gallus DNA polymerase eta (POLH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLH ( NM_001001304.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001001304.2 Gallus gallus DNA polymerase eta (POLH), mRNA.

Price & Availability↑

CloneID	OGa28328
Clone ID Related Accession (Same CDS sequence)	NM_001001304.2
Accession Version	NM_001001304.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-01
Organism	Gallus gallus(chicken)
Product	DNA polymerase eta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ399290.1, AJ720665.1 and BU255275.1. On Dec 31, 2014 this sequence version replaced NM_001001304.1.

ATGTCGCGGG GGCGGGAGCG GGTGGTGGCC TTGGTGGACA TGGACTGCTT CTTCATGCAG 
GTGGAGCAGC GCTTCGACCC GCGGCTGCGC GGCCGGCCCT GCGCCGTGGT GCAGTACAAC 
CAGTGGCAGG GCGGCGGGAT CATCGCGGTG AGCTACGAGG CGCGGGCCTT CGGCGTGTCC 
CGCGGGATGT GGGCGACGGA GGCGCGGGCG CTGTGCCCCG AGCTGCTGCT GGCGCGGGTG 
CCCGAGGCGC GGGGCAAAGC CGACCTCACC CGGTACCGGG AGGCCAGCGC CGAGGTGATG 
GAGGTGCTGT CGCGCTTCGC CGCCATCGAG CGGGCCAGCA TCGACGAGGC GTACCTGGAC 
CTGACGGGCA GCGCGCGGGA GCGGCTGCGG GAGCTGCGGG GGCGGCCCCT GGAGGCCGAG 
CTGCTGCCCA CCACCTTCGT GCAGGGGCTG CCCGCCGAGC CCGGCCTCCA GCCGGCCGAC 
AAGGAGGAGC TGCGGCGGCG TGGCTTGCAG GAGTGGCTGG CGTCGCTGTC TTTCGATAAC 
GTCAACTGCC CCGACCTGCA GCTGGCCATG GGAGCAGTCA TTGTGGAGGA AATAAGGGTG 
GCTGTGGAGA AGGCCACCGG ATTCAGGTGC TCGGCTGGGA TTTCGCACAA CAAAATGCTG 
GCAAAACTGG CCTGTGGGTT AAACAAGCCC AACCGCCAGA CACTGGTATC TTCACGATCT 
GTCCCGCAGC TCTTCAGCCA GATGCCTGTC AGCAGTATCC GTAACCTGGG GGGCAAGCTT 
GGTGTTGCCA TCACAGACAT CCTGGGAGTG GAGTACATCG GGGAGGTGAC GAAGTTCAGT 
GAGATGGAGC TCCAGACTCA CTTTGGAGAC AAGACTGGGT CCTGGCTCTA TGATCTGTGC 
AGAGGAATTG ATGATGAACC TGTCAAGAAC CGCCACTTGC CTCAGTCCAT TGGCTGCAGC 
AAGAACTTCC CTGGGAAAAC AGCCCTGGCT ACACAGAAGG AGGTGCAACA CTGGCTGCTG 
CAGCTGGCCT TGGAGCTGGA ATCCAGGCTG ATCAAGGACA GGAGTCAGAA CCACCGAGTG 
GCCAAGCAGC TGATGGTGGT CATTCGCATG CAAGGGGACA CCAGGCTGTC ACGCTTTTGT 
GCTGTGACCC GCTACGATGC CCAGAAGATC TTCAATGATG CCTTTGCCCT CATCCAAAAC 
TGCAACATGG CTGGGGCTCA TCAGGCAGCC TGGTCTCCGC CACTCATATC AGTGCACCTC 
GCAGCTAGCA AGTTCTCTGC TCCCACATTC CTCTCTGCAG GCATCGCCTC CTTTCTGACT 
AGTGACACTT CATCTGATGG CACTGATGCT GGCAGCACAG AGGCAACATC TTCCACGAGG 
CCTAGGGTCA AATTTTTCAG GAGCCCAAGC AAAGAGCATA GACAGAAGCC GGCTAATGCT 
ATTGAGTCGT TCTTCCAAAA GGTGGCAGAA AGGCAGCAGT CGCAGGCAGT ACCAAGCGGC 
CTGCCAGCTG CTACCCATGC TCACTCACCC GCAGCCAGCT CCATGGAGCA CCCAGAGGGA 
GACAGCGCTG GACTTGCCTC TGAGCAGCGT GAGCTGGAGG CCTCTGTGAA GCAGAGTCCC 
AGAGATGGGA GCCCTGCTTC TCCTTGCAAG AGGCCCCCTT GTGAGGAGCT GCTGTCTGAC 
GCTACACAAA CACCCTCAAC TCCACCCAGC TCCAGGATCC TTCCGAAGTT AAAGCCAGCT 
TTGGAGGGAA ATGAGCAGAA TTTGCCACCC CCTGAGCAAG CCCTGCTCCC TCATGTCTCA 
CCAGGGGACC AGCAGTGCTG TGAGAAGTGT GGCCAGTATG TGTTGGCCTG GGAGCTCCCA 
GAGCACATGG ACTACCACTT CGCTGTGGAG CTGCAACGCT CCTTCCAGGA GCCCAGCTCT 
CCACCAGCTC TAGCAGAGGT TCCCATCGCC AAGGCTGCAG CTTCTGGGTC TCCTGCCAAG 
GTGAGGAACA AGGCCAAGGC TCCAGCAGGA CCTAATGCAA AACGACCTCG GGAAAGCGTG 
GCAAGGACTT TAGATTTTTT CTTTAAGCGC TTACCTCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001304.2
CDS	23..2104
Translation	MSRGRERVVALVDMDCFFMQVEQRFDPRLRGRPCAVVQYNQWQGGGIIAVSYEARAFGVSRGMWATEARALCPELLLARVPEARGKADLTRYREASAEVMEVLSRFAAIERASIDEAYLDLTGSARERLRELRGRPLEAELLPTTFVQGLPAEPGLQPADKEELRRRGLQEWLASLSFDNVNCPDLQLAMGAVIVEEIRVAVEKATGFRCSAGISHNKMLAKLACGLNKPNRQTLVSSRSVPQLFSQMPVSSIRNLGGKLGVAITDILGVEYIGEVTKFSEMELQTHFGDKTGSWLYDLCRGIDDEPVKNRHLPQSIGCSKNFPGKTALATQKEVQHWLLQLALELESRLIKDRSQNHRVAKQLMVVIRMQGDTRLSRFCAVTRYDAQKIFNDAFALIQNCNMAGAHQAAWSPPLISVHLAASKFSAPTFLSAGIASFLTSDTSSDGTDAGSTEATSSTRPRVKFFRSPSKEHRQKPANAIESFFQKVAERQQSQAVPSGLPAATHAHSPAASSMEHPEGDSAGLASEQRELEASVKQSPRDGSPASPCKRPPCEELLSDATQTPSTPPSSRILPKLKPALEGNEQNLPPPEQALLPHVSPGDQQCCEKCGQYVLAWELPEHMDYHFAVELQRSFQEPSSPPALAEVPIAKAAASGSPAKVRNKAKAPAGPNAKRPRESVARTLDFFFKRLPP

Target ORF information:

RefSeq Version	NM_001001304.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA polymerase eta (POLH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001304.2

ORF Insert Sequence:

ATGTCGCGGG GGCGGGAGCG GGTGGTGGCC TTGGTGGACA TGGACTGCTT CTTCATGCAG 
GTGGAGCAGC GCTTCGACCC GCGGCTGCGC GGCCGGCCCT GCGCCGTGGT GCAGTACAAC 
CAGTGGCAGG GCGGCGGGAT CATCGCGGTG AGCTACGAGG CGCGGGCCTT CGGCGTGTCC 
CGCGGGATGT GGGCGACGGA GGCGCGGGCG CTGTGCCCCG AGCTGCTGCT GGCGCGGGTG 
CCCGAGGCGC GGGGCAAAGC CGACCTCACC CGGTACCGGG AGGCCAGCGC CGAGGTGATG 
GAGGTGCTGT CGCGCTTCGC CGCCATCGAG CGGGCCAGCA TCGACGAGGC GTACCTGGAC 
CTGACGGGCA GCGCGCGGGA GCGGCTGCGG GAGCTGCGGG GGCGGCCCCT GGAGGCCGAG 
CTGCTGCCCA CCACCTTCGT GCAGGGGCTG CCCGCCGAGC CCGGCCTCCA GCCGGCCGAC 
AAGGAGGAGC TGCGGCGGCG TGGCTTGCAG GAGTGGCTGG CGTCGCTGTC TTTCGATAAC 
GTCAACTGCC CCGACCTGCA GCTGGCCATG GGAGCAGTCA TTGTGGAGGA AATAAGGGTG 
GCTGTGGAGA AGGCCACCGG ATTCAGGTGC TCGGCTGGGA TTTCGCACAA CAAAATGCTG 
GCAAAACTGG CCTGTGGGTT AAACAAGCCC AACCGCCAGA CACTGGTATC TTCACGATCT 
GTCCCGCAGC TCTTCAGCCA GATGCCTGTC AGCAGTATCC GTAACCTGGG GGGCAAGCTT 
GGTGTTGCCA TCACAGACAT CCTGGGAGTG GAGTACATCG GGGAGGTGAC GAAGTTCAGT 
GAGATGGAGC TCCAGACTCA CTTTGGAGAC AAGACTGGGT CCTGGCTCTA TGATCTGTGC 
AGAGGAATTG ATGATGAACC TGTCAAGAAC CGCCACTTGC CTCAGTCCAT TGGCTGCAGC 
AAGAACTTCC CTGGGAAAAC AGCCCTGGCT ACACAGAAGG AGGTGCAACA CTGGCTGCTG 
CAGCTGGCCT TGGAGCTGGA ATCCAGGCTG ATCAAGGACA GGAGTCAGAA CCACCGAGTG 
GCCAAGCAGC TGATGGTGGT CATTCGCATG CAAGGGGACA CCAGGCTGTC ACGCTTTTGT 
GCTGTGACCC GCTACGATGC CCAGAAGATC TTCAATGATG CCTTTGCCCT CATCCAAAAC 
TGCAACATGG CTGGGGCTCA TCAGGCAGCC TGGTCTCCGC CACTCATATC AGTGCACCTC 
GCAGCTAGCA AGTTCTCTGC TCCCACATTC CTCTCTGCAG GCATCGCCTC CTTTCTGACT 
AGTGACACTT CATCTGATGG CACTGATGCT GGCAGCACAG AGGCAACATC TTCCACGAGG 
CCTAGGGTCA AATTTTTCAG GAGCCCAAGC AAAGAGCATA GACAGAAGCC GGCTAATGCT 
ATTGAGTCGT TCTTCCAAAA GGTGGCAGAA AGGCAGCAGT CGCAGGCAGT ACCAAGCGGC 
CTGCCAGCTG CTACCCATGC TCACTCACCC GCAGCCAGCT CCATGGAGCA CCCAGAGGGA 
GACAGCGCTG GACTTGCCTC TGAGCAGCGT GAGCTGGAGG CCTCTGTGAA GCAGAGTCCC 
AGAGATGGGA GCCCTGCTTC TCCTTGCAAG AGGCCCCCTT GTGAGGAGCT GCTGTCTGAC 
GCTACACAAA CACCCTCAAC TCCACCCAGC TCCAGGATCC TTCCGAAGTT AAAGCCAGCT 
TTGGAGGGAA ATGAGCAGAA TTTGCCACCC CCTGAGCAAG CCCTGCTCCC TCATGTCTCA 
CCAGGGGACC AGCAGTGCTG TGAGAAGTGT GGCCAGTATG TGTTGGCCTG GGAGCTCCCA 
GAGCACATGG ACTACCACTT CGCTGTGGAG CTGCAACGCT CCTTCCAGGA GCCCAGCTCT 
CCACCAGCTC TAGCAGAGGT TCCCATCGCC AAGGCTGCAG CTTCTGGGTC TCCTGCCAAG 
GTGAGGAACA AGGCCAAGGC TCCAGCAGGA CCTAATGCAA AACGACCTCG GGAAAGCGTG 
GCAAGGACTT TAGATTTTTT CTTTAAGCGC TTACCTCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TopBP1-mediated DNA processing during mitosis.
Cell cycle (Georgetown, Tex.)15(2)176-83(2016)
Gallina I,Christiansen SK,Pedersen RT,Lisby M,Oestergaard VH

GCN5 protects vertebrate cells against UV-irradiation via controlling gene expression of DNA polymerase ?.
The Journal of biological chemistry287(47)39842-9(2012 Nov)
Kikuchi H,Kuribayashi F,Imajoh-Ohmi S,Nishitoh H,Takami Y,Nakayama T

Simultaneous disruption of two DNA polymerases, Pol? and Pol?, in Avian DT40 cells unmasks the role of Pol? in cellular response to various DNA lesions.
PLoS genetics6(10)(2010 Oct)
Hirota K,Sonoda E,Kawamoto T,Motegi A,Masutani C,Hanaoka F,Sz?ts D,Iwai S,Sale JE,Lehmann A,Takeda S

Dual roles for DNA polymerase eta in homologous DNA recombination and translesion DNA synthesis.
Molecular cell20(5)793-9(2005 Dec)
Kawamoto T,Araki K,Sonoda E,Yamashita YM,Harada K,Kikuchi K,Masutani C,Hanaoka F,Nozaki K,Hashimoto N,Takeda S

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

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POLH cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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