Pus7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pus7
Entrez Gene ID	78697
Full Name	pseudouridylate synthase 7
Synonyms	C330017I15Rik
General protein information	Preferred Names pseudouridylate synthase 7 Names pseudouridylate synthase 7 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011249796.1	XP_011248098.1	pseudouridylate synthase 7 homolog isoform X1
NM_001289780.1	NP_001276709.1	pseudouridylate synthase 7 homolog isoform 1
XM_030254910.1	XP_030110770.1	pseudouridylate synthase 7 homolog isoform X2
NM_178403.5	NP_848490.2	pseudouridylate synthase 7 homolog isoform 2
NM_001289781.1	NP_001276710.1	pseudouridylate synthase 7 homolog isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PUS7	XP_003318781.1
Rattus norvegicus (Norway rat)	Pus7	NP_001164060.1
Kluyveromyces lactis	KLLA0F11253g	XP_455592.1
Schizosaccharomyces pombe (fission yeast)	SPBC1A4.09	NP_595812.1
	PUS7	XP_001089755.2
Homo sapiens (human)	PUS7	NP_061915.2
Canis lupus familiaris (dog)	PUS7	XP_005631019.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005743	XP_556395.3
Saccharomyces cerevisiae (baker's yeast)	PUS7	NP_014886.1
Eremothecium gossypii	AGOS_ACR136C	NP_983538.1
Oryza sativa (rice)	Os05g0509300	NP_001174483.1
Mus musculus (house mouse)	Pus7	NP_848490.2
Gallus gallus (chicken)	PUS7	NP_001186468.1
Danio rerio (zebrafish)	pus7	NP_001116170.1
Arabidopsis thaliana (thale cress)	AT3G04820	NP_187133.3
Bos taurus (cattle)	PUS7	NP_001070407.1
Drosophila melanogaster (fruit fly)	CG6745	NP_648231.1
Xenopus tropicalis (tropical clawed frog)	pus7	NP_001016884.1

Gene Ontology
Bibliography

Related articles in PubMed

Misregulation of an Activity-Dependent Splicing Network as a Common Mechanism Underlying Autism Spectrum Disorders.
Quesnel-Valli?res M, Dargaei Z, Irimia M, Gonatopoulos-Pournatzis T, Ip JY, Wu M, Sterne-Weiler T, Nakagawa S, Woodin MA, Blencowe BJ, Cordes SP
Molecular cell64(6)1023-1034(2016 Dec)

Essential roles for the splicing regulator nSR100/SRRM4 during nervous system development.
Quesnel-Valli?res M, Irimia M, Cordes SP, Blencowe BJ
Genes & development29(7)746-59(2015 Apr)

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, Hoshino K, Kitamura H, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)

A catalogue of genes in mouse embryonal carcinoma F9 cells identified with expressed sequence tags.
Nishiguchi S, Sakuma R, Nomura M, Zou Z, Jearanaisilavong J, Joh T, Yasunaga T, Shimada K
Journal of biochemistry119(4)749-67(1996 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pus7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pus7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu58320	NM_178403.5 Latest version!	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu58320	NM_001289781.1 Latest version!	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu71736	XM_011249796.1 Latest version!	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu89043	XM_030254910.1 Latest version!	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu58321	NM_001289780.1 Latest version!	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pus7 ( NM_178403.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848490.2 Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu58320
Clone ID Related Accession (Same CDS sequence)	NM_178403.5 , NM_001289781.1
Accession Version	NM_178403.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	pseudouridylate synthase 7 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY067917.1, BY065820.1, AK160849.1, AK145380.1, AC117614.14 and CD741583.1. On Jan 31, 2014 this sequence version replaced NM_178403.4. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which lacks an internal segment and is shorter, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK160849.1, BC008544.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAATC CAAGAAAACA TTCTTGGCCA AAATCCCGGG GAAGTTACTG CCATTTTGTT 
CTCTACAAAG AAAACAAAGA CACCATGGAT GCCATCAATG TTCTATCCAA GTACCTGCGG 
GTAAAGCCAA ACATATTCTC CTATATGGGA ACCAAAGACA AAAGGGCAAT CACAGTCCAG 
GAGATTGCTG TTCTCAAAAT AAGTGCACAA AGACTTGCCC ACCTCAATAA GTGCCTGATG 
AACTTGAAGC TCGGGAATTT CAGCTATCAG AAAACCCCTC TGAAGTTAGG AGCGCTCCAA 
GGAAACCACT TCACTGTTGT TCTCAGGAAT ATAACAGGAA CTGATGAGCA GGTGCAGCAA 
GCCATGCAGT CTCTGAGGGA GACCGGCTTC ATTAACTACT ATGGCATGCA AAGGTTCGGG 
ACCACAGCTG TTCCAACGTA CCAGGTTGGA AGAGCAATTC TACAGAATTC CTGGACAGAA 
GTCATGGATT TAATACTGAA ACCCCGTTCT GGAGCTGAGA AGGGTTACTT GGTGAAATGC 
AGAGAAGAAT GGGCCAAGAC CAAAGACCCA GCCTCTGCTC TCAAAAAGCT GCCCGTCAAA 
AGGTGTGTGG AAGGGCAGCT GCTCCGAGGA CTTTCCAGAT ATGGAATGAA GAATATAGTC 
TCTGCATTTG GCATAATTCC AAGAAACAAT CGCTTAATGT ACATCCACAG TTATCAGAGC 
TACGTGTGGA ACACCATGGT GAGCAGAAGA ATTGAGGAGT ACGGGCTGAG GCCTGTGCCC 
GGGGACCTTG TTCTCAAAGG AGCCACGCCC ACCTACATTG AGGAAGATGA CGTTGACAAC 
TACTCCATCC ACGATGTGGT GATGCCTTTG CCTGGGTTTG ATGTTATCTA CCCCAAGCAT 
AAAATCAGCG AGGCCTACAG GGAAATGCTT GCTGCAGACA ACCTTGATAT TGACAACATG 
AGACACACGA TTCGAGATTA CTCCTTATCG GGGGCCTACC GAAGGATCAT CATCAGGCCT 
CAGAGCGTCA GCTGGGAAGT TGTTGCATAT GATGATCCTA AAATTCCACT TTTCAACACG 
GATGTGGACA ACCTCGAGGG GAAACCACCA CCAGTGTTTG CTTCTGAAGG CAAATACAGG 
GCTCTGAAGA TGGACTTTTC TCTTCCTCCT TCTACTTACG CCACCATGGC CATTCGAGAA 
GTGCTCAAAA TGGACACCAG CATCAAGAAC CAGACCCAGC TAAATACGAG CTGGCTGCGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848490.2
CDS	233..2215
Translation	MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDENSSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHEIGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIEDTKEKRTVIHQAIKSLFPGLETKTEDREGRKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLRVKPNIFSYMGTKDKRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRNITGTDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVKCREEWAKTKDPASALKKLPVKRCVEGQLLRGLSRYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNTMVSRRIEEYGLRPVPGDLVLKGATPTYIEEDDVDNYSIHDVVMPLPGFDVIYPKHKISEAYREMLAADNLDIDNMRHTIRDYSLSGAYRRIIIRPQSVSWEVVAYDDPKIPLFNTDVDNLEGKPPPVFASEGKYRALKMDFSLPPSTYATMAIREVLKMDTSIKNQTQLNTSWLR

Target ORF information:

RefSeq Version	NM_178403.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178403.5

ORF Insert Sequence:

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAATC CAAGAAAACA TTCTTGGCCA AAATCCCGGG GAAGTTACTG CCATTTTGTT 
CTCTACAAAG AAAACAAAGA CACCATGGAT GCCATCAATG TTCTATCCAA GTACCTGCGG 
GTAAAGCCAA ACATATTCTC CTATATGGGA ACCAAAGACA AAAGGGCAAT CACAGTCCAG 
GAGATTGCTG TTCTCAAAAT AAGTGCACAA AGACTTGCCC ACCTCAATAA GTGCCTGATG 
AACTTGAAGC TCGGGAATTT CAGCTATCAG AAAACCCCTC TGAAGTTAGG AGCGCTCCAA 
GGAAACCACT TCACTGTTGT TCTCAGGAAT ATAACAGGAA CTGATGAGCA GGTGCAGCAA 
GCCATGCAGT CTCTGAGGGA GACCGGCTTC ATTAACTACT ATGGCATGCA AAGGTTCGGG 
ACCACAGCTG TTCCAACGTA CCAGGTTGGA AGAGCAATTC TACAGAATTC CTGGACAGAA 
GTCATGGATT TAATACTGAA ACCCCGTTCT GGAGCTGAGA AGGGTTACTT GGTGAAATGC 
AGAGAAGAAT GGGCCAAGAC CAAAGACCCA GCCTCTGCTC TCAAAAAGCT GCCCGTCAAA 
AGGTGTGTGG AAGGGCAGCT GCTCCGAGGA CTTTCCAGAT ATGGAATGAA GAATATAGTC 
TCTGCATTTG GCATAATTCC AAGAAACAAT CGCTTAATGT ACATCCACAG TTATCAGAGC 
TACGTGTGGA ACACCATGGT GAGCAGAAGA ATTGAGGAGT ACGGGCTGAG GCCTGTGCCC 
GGGGACCTTG TTCTCAAAGG AGCCACGCCC ACCTACATTG AGGAAGATGA CGTTGACAAC 
TACTCCATCC ACGATGTGGT GATGCCTTTG CCTGGGTTTG ATGTTATCTA CCCCAAGCAT 
AAAATCAGCG AGGCCTACAG GGAAATGCTT GCTGCAGACA ACCTTGATAT TGACAACATG 
AGACACACGA TTCGAGATTA CTCCTTATCG GGGGCCTACC GAAGGATCAT CATCAGGCCT 
CAGAGCGTCA GCTGGGAAGT TGTTGCATAT GATGATCCTA AAATTCCACT TTTCAACACG 
GATGTGGACA ACCTCGAGGG GAAACCACCA CCAGTGTTTG CTTCTGAAGG CAAATACAGG 
GCTCTGAAGA TGGACTTTTC TCTTCCTCCT TCTACTTACG CCACCATGGC CATTCGAGAA 
GTGCTCAAAA TGGACACCAG CATCAAGAAC CAGACCCAGC TAAATACGAG CTGGCTGCGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pus7 ( NM_001289781.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276710.1 Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu58320
Clone ID Related Accession (Same CDS sequence)	NM_178403.5 , NM_001289781.1
Accession Version	NM_001289781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	pseudouridylate synthase 7 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117614.14, AK160849.1, AK145380.1 and CD741583.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which lacks an internal segment and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK145380.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAATC CAAGAAAACA TTCTTGGCCA AAATCCCGGG GAAGTTACTG CCATTTTGTT 
CTCTACAAAG AAAACAAAGA CACCATGGAT GCCATCAATG TTCTATCCAA GTACCTGCGG 
GTAAAGCCAA ACATATTCTC CTATATGGGA ACCAAAGACA AAAGGGCAAT CACAGTCCAG 
GAGATTGCTG TTCTCAAAAT AAGTGCACAA AGACTTGCCC ACCTCAATAA GTGCCTGATG 
AACTTGAAGC TCGGGAATTT CAGCTATCAG AAAACCCCTC TGAAGTTAGG AGCGCTCCAA 
GGAAACCACT TCACTGTTGT TCTCAGGAAT ATAACAGGAA CTGATGAGCA GGTGCAGCAA 
GCCATGCAGT CTCTGAGGGA GACCGGCTTC ATTAACTACT ATGGCATGCA AAGGTTCGGG 
ACCACAGCTG TTCCAACGTA CCAGGTTGGA AGAGCAATTC TACAGAATTC CTGGACAGAA 
GTCATGGATT TAATACTGAA ACCCCGTTCT GGAGCTGAGA AGGGTTACTT GGTGAAATGC 
AGAGAAGAAT GGGCCAAGAC CAAAGACCCA GCCTCTGCTC TCAAAAAGCT GCCCGTCAAA 
AGGTGTGTGG AAGGGCAGCT GCTCCGAGGA CTTTCCAGAT ATGGAATGAA GAATATAGTC 
TCTGCATTTG GCATAATTCC AAGAAACAAT CGCTTAATGT ACATCCACAG TTATCAGAGC 
TACGTGTGGA ACACCATGGT GAGCAGAAGA ATTGAGGAGT ACGGGCTGAG GCCTGTGCCC 
GGGGACCTTG TTCTCAAAGG AGCCACGCCC ACCTACATTG AGGAAGATGA CGTTGACAAC 
TACTCCATCC ACGATGTGGT GATGCCTTTG CCTGGGTTTG ATGTTATCTA CCCCAAGCAT 
AAAATCAGCG AGGCCTACAG GGAAATGCTT GCTGCAGACA ACCTTGATAT TGACAACATG 
AGACACACGA TTCGAGATTA CTCCTTATCG GGGGCCTACC GAAGGATCAT CATCAGGCCT 
CAGAGCGTCA GCTGGGAAGT TGTTGCATAT GATGATCCTA AAATTCCACT TTTCAACACG 
GATGTGGACA ACCTCGAGGG GAAACCACCA CCAGTGTTTG CTTCTGAAGG CAAATACAGG 
GCTCTGAAGA TGGACTTTTC TCTTCCTCCT TCTACTTACG CCACCATGGC CATTCGAGAA 
GTGCTCAAAA TGGACACCAG CATCAAGAAC CAGACCCAGC TAAATACGAG CTGGCTGCGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276710.1
CDS	584..2566
Translation	MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDENSSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHEIGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIEDTKEKRTVIHQAIKSLFPGLETKTEDREGRKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLRVKPNIFSYMGTKDKRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRNITGTDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVKCREEWAKTKDPASALKKLPVKRCVEGQLLRGLSRYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNTMVSRRIEEYGLRPVPGDLVLKGATPTYIEEDDVDNYSIHDVVMPLPGFDVIYPKHKISEAYREMLAADNLDIDNMRHTIRDYSLSGAYRRIIIRPQSVSWEVVAYDDPKIPLFNTDVDNLEGKPPPVFASEGKYRALKMDFSLPPSTYATMAIREVLKMDTSIKNQTQLNTSWLR

Target ORF information:

RefSeq Version	NM_001289781.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289781.1

ORF Insert Sequence:

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAATC CAAGAAAACA TTCTTGGCCA AAATCCCGGG GAAGTTACTG CCATTTTGTT 
CTCTACAAAG AAAACAAAGA CACCATGGAT GCCATCAATG TTCTATCCAA GTACCTGCGG 
GTAAAGCCAA ACATATTCTC CTATATGGGA ACCAAAGACA AAAGGGCAAT CACAGTCCAG 
GAGATTGCTG TTCTCAAAAT AAGTGCACAA AGACTTGCCC ACCTCAATAA GTGCCTGATG 
AACTTGAAGC TCGGGAATTT CAGCTATCAG AAAACCCCTC TGAAGTTAGG AGCGCTCCAA 
GGAAACCACT TCACTGTTGT TCTCAGGAAT ATAACAGGAA CTGATGAGCA GGTGCAGCAA 
GCCATGCAGT CTCTGAGGGA GACCGGCTTC ATTAACTACT ATGGCATGCA AAGGTTCGGG 
ACCACAGCTG TTCCAACGTA CCAGGTTGGA AGAGCAATTC TACAGAATTC CTGGACAGAA 
GTCATGGATT TAATACTGAA ACCCCGTTCT GGAGCTGAGA AGGGTTACTT GGTGAAATGC 
AGAGAAGAAT GGGCCAAGAC CAAAGACCCA GCCTCTGCTC TCAAAAAGCT GCCCGTCAAA 
AGGTGTGTGG AAGGGCAGCT GCTCCGAGGA CTTTCCAGAT ATGGAATGAA GAATATAGTC 
TCTGCATTTG GCATAATTCC AAGAAACAAT CGCTTAATGT ACATCCACAG TTATCAGAGC 
TACGTGTGGA ACACCATGGT GAGCAGAAGA ATTGAGGAGT ACGGGCTGAG GCCTGTGCCC 
GGGGACCTTG TTCTCAAAGG AGCCACGCCC ACCTACATTG AGGAAGATGA CGTTGACAAC 
TACTCCATCC ACGATGTGGT GATGCCTTTG CCTGGGTTTG ATGTTATCTA CCCCAAGCAT 
AAAATCAGCG AGGCCTACAG GGAAATGCTT GCTGCAGACA ACCTTGATAT TGACAACATG 
AGACACACGA TTCGAGATTA CTCCTTATCG GGGGCCTACC GAAGGATCAT CATCAGGCCT 
CAGAGCGTCA GCTGGGAAGT TGTTGCATAT GATGATCCTA AAATTCCACT TTTCAACACG 
GATGTGGACA ACCTCGAGGG GAAACCACCA CCAGTGTTTG CTTCTGAAGG CAAATACAGG 
GCTCTGAAGA TGGACTTTTC TCTTCCTCCT TCTACTTACG CCACCATGGC CATTCGAGAA 
GTGCTCAAAA TGGACACCAG CATCAAGAAC CAGACCCAGC TAAATACGAG CTGGCTGCGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pus7 ( XM_011249796.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248098.1 Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu71736
Clone ID Related Accession (Same CDS sequence)	XM_011249796.1
Accession Version	XM_011249796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pseudouridylate synthase 7 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAAGG TCAGAGCTGC TGCAGATCCA AGAAAACATT CTTGGCCAAA ATCCCGGGGA 
AGTTACTGCC ATTTTGTTCT CTACAAAGAA AACAAAGACA CCATGGATGC CATCAATGTT 
CTATCCAAGT ACCTGCGGGT AAAGCCAAAC ATATTCTCCT ATATGGGAAC CAAAGACAAA 
AGGGCAATCA CAGTCCAGGA GATTGCTGTT CTCAAAATAA GTGCACAAAG ACTTGCCCAC 
CTCAATAAGT GCCTGATGAA CTTGAAGCTC GGGAATTTCA GCTATCAGAA AACCCCTCTG 
AAGTTAGGAG CGCTCCAAGG AAACCACTTC ACTGTTGTTC TCAGGAATAT AACAGGAACT 
GATGAGCAGG TGCAGCAAGC CATGCAGTCT CTGAGGGAGA CCGGCTTCAT TAACTACTAT 
GGCATGCAAA GGTTCGGGAC CACAGCTGTT CCAACGTACC AGGTTGGAAG GAGCTGGCAC 
GCTGGAGTGC ACACCGAGTG TGGTCTGAGA ACATGTCTTC CTGGTTCTGT CTCAGCACTG 
GCAGGCGGAG GCTGCGCTTC AGAAGGGCCG AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248098.1
CDS	227..1522
Translation	MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDENSSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHEIGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIEDTKEKRTVIHQAIKSLFPGLETKTEDREGRKYIVAYHAAGKKALAKVRAAADPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLRVKPNIFSYMGTKDKRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRNITGTDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRSWHAGVHTECGLRTCLPGSVSALAGGGCASEGPN

Target ORF information:

RefSeq Version	XM_011249796.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249796.1

ORF Insert Sequence:

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAAGG TCAGAGCTGC TGCAGATCCA AGAAAACATT CTTGGCCAAA ATCCCGGGGA 
AGTTACTGCC ATTTTGTTCT CTACAAAGAA AACAAAGACA CCATGGATGC CATCAATGTT 
CTATCCAAGT ACCTGCGGGT AAAGCCAAAC ATATTCTCCT ATATGGGAAC CAAAGACAAA 
AGGGCAATCA CAGTCCAGGA GATTGCTGTT CTCAAAATAA GTGCACAAAG ACTTGCCCAC 
CTCAATAAGT GCCTGATGAA CTTGAAGCTC GGGAATTTCA GCTATCAGAA AACCCCTCTG 
AAGTTAGGAG CGCTCCAAGG AAACCACTTC ACTGTTGTTC TCAGGAATAT AACAGGAACT 
GATGAGCAGG TGCAGCAAGC CATGCAGTCT CTGAGGGAGA CCGGCTTCAT TAACTACTAT 
GGCATGCAAA GGTTCGGGAC CACAGCTGTT CCAACGTACC AGGTTGGAAG GAGCTGGCAC 
GCTGGAGTGC ACACCGAGTG TGGTCTGAGA ACATGTCTTC CTGGTTCTGT CTCAGCACTG 
GCAGGCGGAG GCTGCGCTTC AGAAGGGCCG AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pus7 ( XM_030254910.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110770.1 Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant X3, mRNA.

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CloneID	OMu89043
Clone ID Related Accession (Same CDS sequence)	XM_030254910.1
Accession Version	XM_030254910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pseudouridylate synthase 7 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAATC CAAGAAAACA TTCTTGGCCA AAATCCCGGG GAAGTTACTG CCATTTTGTT 
CTCTACAAAG AAAACAAAGA CACCATGGAT GCCATCAATG TTCTATCCAA GTACCTGCGG 
GTAAAGCCAA ACATATTCTC CTATATGGGA ACCAAAGACA AAAGGGCAAT CACAGTCCAG 
GAGATTGCTG TTCTCAAAAT AAGTGCACAA AGACTTGCCC ACCTCAATAA GTGCCTGATG 
AACTTGAAGC TCGGGAATTT CAGCTATCAG AAAACCCCTC TGAAGTTAGG AGCGCTCCAA 
GGAAACCACT TCACTGTTGT TCTCAGGAAT ATAACAGGAA CTGATGAGCA GGTGCAGCAA 
GCCATGCAGT CTCTGAGGGA GACCGGCTTC ATTAACTACT ATGGCATGCA AAGGTTCGGG 
ACCACAGCTG TTCCAACGTA CCAGGTTGGA AGGAGCTGGC ACGCTGGAGT GCACACCGAG 
TGTGGTCTGA GAACATGTCT TCCTGGTTCT GTCTCAGCAC TGGCAGGCGG AGGCTGCGCT 
TCAGAAGGGC CGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110770.1
CDS	227..1504
Translation	MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDENSSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHEIGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIEDTKEKRTVIHQAIKSLFPGLETKTEDREGRKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLRVKPNIFSYMGTKDKRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRNITGTDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRSWHAGVHTECGLRTCLPGSVSALAGGGCASEGPN

Target ORF information:

RefSeq Version	XM_030254910.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254910.1

ORF Insert Sequence:

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAATC CAAGAAAACA TTCTTGGCCA AAATCCCGGG GAAGTTACTG CCATTTTGTT 
CTCTACAAAG AAAACAAAGA CACCATGGAT GCCATCAATG TTCTATCCAA GTACCTGCGG 
GTAAAGCCAA ACATATTCTC CTATATGGGA ACCAAAGACA AAAGGGCAAT CACAGTCCAG 
GAGATTGCTG TTCTCAAAAT AAGTGCACAA AGACTTGCCC ACCTCAATAA GTGCCTGATG 
AACTTGAAGC TCGGGAATTT CAGCTATCAG AAAACCCCTC TGAAGTTAGG AGCGCTCCAA 
GGAAACCACT TCACTGTTGT TCTCAGGAAT ATAACAGGAA CTGATGAGCA GGTGCAGCAA 
GCCATGCAGT CTCTGAGGGA GACCGGCTTC ATTAACTACT ATGGCATGCA AAGGTTCGGG 
ACCACAGCTG TTCCAACGTA CCAGGTTGGA AGGAGCTGGC ACGCTGGAGT GCACACCGAG 
TGTGGTCTGA GAACATGTCT TCCTGGTTCT GTCTCAGCAC TGGCAGGCGG AGGCTGCGCT 
TCAGAAGGGC CGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pus7 ( NM_001289780.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276709.1 Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu58321
Clone ID Related Accession (Same CDS sequence)	NM_001289780.1
Accession Version	NM_001289780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	pseudouridylate synthase 7 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY067917.1, BY065820.1, AK142271.1, AC117614.14, AK145380.1 and CD741583.1. On Jan 31, 2014 this sequence version replaced XM_006535815.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC145316.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAAGG TCAGAGCTGC TGCAGATCCA AGAAAACATT CTTGGCCAAA ATCCCGGGGA 
AGTTACTGCC ATTTTGTTCT CTACAAAGAA AACAAAGACA CCATGGATGC CATCAATGTT 
CTATCCAAGT ACCTGCGGGT AAAGCCAAAC ATATTCTCCT ATATGGGAAC CAAAGACAAA 
AGGGCAATCA CAGTCCAGGA GATTGCTGTT CTCAAAATAA GTGCACAAAG ACTTGCCCAC 
CTCAATAAGT GCCTGATGAA CTTGAAGCTC GGGAATTTCA GCTATCAGAA AACCCCTCTG 
AAGTTAGGAG CGCTCCAAGG AAACCACTTC ACTGTTGTTC TCAGGAATAT AACAGGAACT 
GATGAGCAGG TGCAGCAAGC CATGCAGTCT CTGAGGGAGA CCGGCTTCAT TAACTACTAT 
GGCATGCAAA GGTTCGGGAC CACAGCTGTT CCAACGTACC AGGTTGGAAG AGCAATTCTA 
CAGAATTCCT GGACAGAAGT CATGGATTTA ATACTGAAAC CCCGTTCTGG AGCTGAGAAG 
GGTTACTTGG TGAAATGCAG AGAAGAATGG GCCAAGACCA AAGACCCAGC CTCTGCTCTC 
AAAAAGCTGC CCGTCAAAAG GTGTGTGGAA GGGCAGCTGC TCCGAGGACT TTCCAGATAT 
GGAATGAAGA ATATAGTCTC TGCATTTGGC ATAATTCCAA GAAACAATCG CTTAATGTAC 
ATCCACAGTT ATCAGAGCTA CGTGTGGAAC ACCATGGTGA GCAGAAGAAT TGAGGAGTAC 
GGGCTGAGGC CTGTGCCCGG GGACCTTGTT CTCAAAGGAG CCACGCCCAC CTACATTGAG 
GAAGATGACG TTGACAACTA CTCCATCCAC GATGTGGTGA TGCCTTTGCC TGGGTTTGAT 
GTTATCTACC CCAAGCATAA AATCAGCGAG GCCTACAGGG AAATGCTTGC TGCAGACAAC 
CTTGATATTG ACAACATGAG ACACACGATT CGAGATTACT CCTTATCGGG GGCCTACCGA 
AGGATCATCA TCAGGCCTCA GAGCGTCAGC TGGGAAGTTG TTGCATATGA TGATCCTAAA 
ATTCCACTTT TCAACACGGA TGTGGACAAC CTCGAGGGGA AACCACCACC AGTGTTTGCT 
TCTGAAGGCA AATACAGGGC TCTGAAGATG GACTTTTCTC TTCCTCCTTC TACTTACGCC 
ACCATGGCCA TTCGAGAAGT GCTCAAAATG GACACCAGCA TCAAGAACCA GACCCAGCTA 
AATACGAGCT GGCTGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276709.1
CDS	233..2233
Translation	MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDENSSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHEIGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIEDTKEKRTVIHQAIKSLFPGLETKTEDREGRKYIVAYHAAGKKALAKVRAAADPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLRVKPNIFSYMGTKDKRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRNITGTDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVKCREEWAKTKDPASALKKLPVKRCVEGQLLRGLSRYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNTMVSRRIEEYGLRPVPGDLVLKGATPTYIEEDDVDNYSIHDVVMPLPGFDVIYPKHKISEAYREMLAADNLDIDNMRHTIRDYSLSGAYRRIIIRPQSVSWEVVAYDDPKIPLFNTDVDNLEGKPPPVFASEGKYRALKMDFSLPPSTYATMAIREVLKMDTSIKNQTQLNTSWLR

Target ORF information:

RefSeq Version	NM_001289780.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pseudouridylate synthase 7 (Pus7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289780.1

ORF Insert Sequence:

ATGGAAATGA CAAGCACGTC CCTGAAACGT GGGTGCCTTG TTGTGGAAGA TAATGACAGT 
GTGACCCCAC ACGACGAAAC AAAGAAACAG AAGGTGTCCG AGGGCTGTCT GACCAGCAGT 
CAGGATGGGG TAGAGAATGA CGGTCTACAC CGCAGTGAGA ACGAGCCTGG GCCTCCAGAA 
GCTGAAAGCA CCGTGAAAGA TGACGAGAAT TCCAGCGCTC AGGTGCAAGA GGAAGAGGAG 
GAGGAGGAGG AAGAAGACGG CCTTTCAGAA GCAGGGGAAG AGGAAGAAGC AGAGAGTTTT 
GCAGACATGA TGAAACATGG GCTCACAGAA CTTGATGTGG GCATCTGCAA GTTCGTGAGT 
TCCCATCACG GCTTCTCGGG GATCTTAAAA GAAAGATACT CTGACTTTGT CGTTCACGAA 
ATCGGGAAAG ATGGACGGAT CAGCCATTTG GATGACTTAT CTGTTCCGGT GGATGAAGAG 
GACCCTCCAG AAGACGCTCT CACTGTCTTG ACAGCTGAGG ACAGGCAGCA GTTGGAGGAG 
CTCCAGCTTT TCAAAAATAA AGAAACCAGT GTTGCCATCG AGGTCATTGA GGACACCAAA 
GAGAAGCGAA CAGTCATCCA TCAAGCCATT AAATCTCTCT TCCCAGGACT AGAGACAAAG 
ACAGAGGATA GAGAGGGGCG GAAATACATC GTAGCCTACC ATGCAGCTGG CAAAAAGGCT 
TTGGCAAAGG TCAGAGCTGC TGCAGATCCA AGAAAACATT CTTGGCCAAA ATCCCGGGGA 
AGTTACTGCC ATTTTGTTCT CTACAAAGAA AACAAAGACA CCATGGATGC CATCAATGTT 
CTATCCAAGT ACCTGCGGGT AAAGCCAAAC ATATTCTCCT ATATGGGAAC CAAAGACAAA 
AGGGCAATCA CAGTCCAGGA GATTGCTGTT CTCAAAATAA GTGCACAAAG ACTTGCCCAC 
CTCAATAAGT GCCTGATGAA CTTGAAGCTC GGGAATTTCA GCTATCAGAA AACCCCTCTG 
AAGTTAGGAG CGCTCCAAGG AAACCACTTC ACTGTTGTTC TCAGGAATAT AACAGGAACT 
GATGAGCAGG TGCAGCAAGC CATGCAGTCT CTGAGGGAGA CCGGCTTCAT TAACTACTAT 
GGCATGCAAA GGTTCGGGAC CACAGCTGTT CCAACGTACC AGGTTGGAAG AGCAATTCTA 
CAGAATTCCT GGACAGAAGT CATGGATTTA ATACTGAAAC CCCGTTCTGG AGCTGAGAAG 
GGTTACTTGG TGAAATGCAG AGAAGAATGG GCCAAGACCA AAGACCCAGC CTCTGCTCTC 
AAAAAGCTGC CCGTCAAAAG GTGTGTGGAA GGGCAGCTGC TCCGAGGACT TTCCAGATAT 
GGAATGAAGA ATATAGTCTC TGCATTTGGC ATAATTCCAA GAAACAATCG CTTAATGTAC 
ATCCACAGTT ATCAGAGCTA CGTGTGGAAC ACCATGGTGA GCAGAAGAAT TGAGGAGTAC 
GGGCTGAGGC CTGTGCCCGG GGACCTTGTT CTCAAAGGAG CCACGCCCAC CTACATTGAG 
GAAGATGACG TTGACAACTA CTCCATCCAC GATGTGGTGA TGCCTTTGCC TGGGTTTGAT 
GTTATCTACC CCAAGCATAA AATCAGCGAG GCCTACAGGG AAATGCTTGC TGCAGACAAC 
CTTGATATTG ACAACATGAG ACACACGATT CGAGATTACT CCTTATCGGG GGCCTACCGA 
AGGATCATCA TCAGGCCTCA GAGCGTCAGC TGGGAAGTTG TTGCATATGA TGATCCTAAA 
ATTCCACTTT TCAACACGGA TGTGGACAAC CTCGAGGGGA AACCACCACC AGTGTTTGCT 
TCTGAAGGCA AATACAGGGC TCTGAAGATG GACTTTTCTC TTCCTCCTTC TACTTACGCC 
ACCATGGCCA TTCGAGAAGT GCTCAAAATG GACACCAGCA TCAAGAACCA GACCCAGCTA 
AATACGAGCT GGCTGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Misregulation of an Activity-Dependent Splicing Network as a Common Mechanism Underlying Autism Spectrum Disorders.
Molecular cell64(6)1023-1034(2016 Dec)
Quesnel-Valli?res M,Dargaei Z,Irimia M,Gonatopoulos-Pournatzis T,Ip JY,Wu M,Sterne-Weiler T,Nakagawa S,Woodin MA,Blencowe BJ,Cordes SP

Essential roles for the splicing regulator nSR100/SRRM4 during nervous system development.
Genes & development29(7)746-59(2015 Apr)
Quesnel-Valli?res M,Irimia M,Cordes SP,Blencowe BJ

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)
Okazaki N,F-Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Seino S,Nishimura M,Kaisho T,Hoshino K,Kitamura H,Nagase T,Ohara O,Koga H

A catalogue of genes in mouse embryonal carcinoma F9 cells identified with expressed sequence tags.
Journal of biochemistry119(4)749-67(1996 Apr)
Nishiguchi S,Sakuma R,Nomura M,Zou Z,Jearanaisilavong J,Joh T,Yasunaga T,Shimada K

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Pus7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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